Detection instrument with the use of polynucleotides mapped on barley chromosome

ABSTRACT

As the results of mass sequencing of cDNA clones originating in barley varieties “ H. spontaneum ”, “Haruna Nijo” and “Akashinriki”, a large number of sites showing single nucleotide polymorphisms (SNPs) among the varieties are found out. Because of involving nonsynonymous substitutions and likely relating to phenotypes inherent in respective varieties, these SNPs are usable for various purposes, for example, genetically distinguishing a variety, isolating a gene, producing/selecting a novel transformant and so on.

This application is US national phase of International application PCT/JP2005/006825, filed 31 Mar. 2005, which designated the U.S. and claims priority of JP 2004-101626 filed 31 Mar. 2004; JP 2004-101662 filed 31 Mar. 2004; JP 2004-101646 filed 31 Mar. 2004; JP 2004-101641 filed 31 Mar. 2004; JP 2004-101618 filed 31 Mar. 2004; JP 2004-101682 filed 31 Mar. 2004; JP 2004-101675 filed 31 Mar. 2004; JP 2004-342598 filed 26 Nov. 2004; JP 2004-342261 filed 26 Nov. 2004; JP 2004-342018 filed 26 Nov. 2004; JP 2004-341944 filed 26 Nov. 2004; JP 2004-342458 filed 26 Nov. 2004; JP 2004-342406 filed 26 Nov. 2004; and JP 2004-342097 filed 26 Nov. 2004, the entire contents of each of which are hereby incorporated by reference.

TECHNICAL FIELD

The present invention relates to a detection instrument that uses polynucleotides mapped on barley chromosomes. Particularly, the invention relates to a detection instrument for detecting, in Triticeae species, gene expression, gene polymorphism, proteins (polypeptides), and substances that interact with proteins (polypeptides).

BACKGROUND ART

Over the last years, the entire genomes of human and many other model organisms have been sequenced. In many of these organisms, sequencing of the entire genomes has been finished already. There has also been ongoing development in the analysis of transcripts and proteins based on the sequence information of genomes. Specifically, transcriptome analysis and proteome analysis have won the recognition. The transcriptome analysis is used for the analysis of transcripts, whereby the expression of all transcripts in an organism or cells are analyzed both systematically and comprehensively. The proteome analysis is a systematic and comprehensive method of analyzing proteins, in which the properties or expression of all proteins expressed at any given location and any given time in an organism or cells are analyzed.

For the systematic and comprehensive analyses, various array techniques are often used. The array technique refers to a technique using an array, in which biosubstances, such as DNA or various proteins obtained from the organism of interest being analyzed, or synthetic substances (for example, compounds with hydrophobic groups or ion exchange groups) that interact with such biosubstances are immobilized on a support in an orderly manner.

With the array technique, the systematic and comprehensive analysis can be performed efficiently. For example, for the analysis of gene transcription control mechanism, it is required to measure transcription level of genes, which varies according to the state of the cell. For this purpose, use of a DNA micro array, one form of the array technique, allows for systematic measurement of transcription level of several thousand to several ten thousand of genes. Among such DNA micro array techniques, one that has been widely used is the DNA micro array technique developed by Affymetrix. In this technique, oligonucleotides are directly synthesized on a silica substrate using a microfabrication technique employed in the fabrication of semiconductors (see Patent Document 1, for example). Meanwhile, arrays have been developed that are modified to detect single nucleotide polymorphism (SNP) (see Non-Patent Documents 1 and 2, for example).

In order to reduce breeding time, labor, and field area, breeding of Triticeae species nowadays employs a method whereby screening is made using genetic markers as an index. Genetic markers have been used since the advent of DNA markers in the late 1980s, and the study of linkage map has advanced greatly with the use of DNA markers. Today, linkage analysis is performed in many organisms based on their high-density linkage maps. Currently, genetic markers that are strongly linked to target traits are available. By using these genetic markers, breeding can be performed more efficiently. The inventors of the present invention have been actively developing genetic markers in Triticeae species. For example, the inventors have proposed (1) a technique concerning genetic markers that are linked to genes conferring aluminum resistance to barley, and use of such genetic markers (see Patent Publication 2), and (2) a technique concerning novel primer sets that are used to detect barley chromosome nucleic acid markers on a wheat background (see Patent Publication 3), and use of such primer sets.

[Patent Publication 1]

Japanese Laid-Open Patent Publication No. 2000-228999 (published on Aug. 22, 2000)

[Patent Publication 2]

Japanese Laid-Open Patent Publication No. 2002-291474 (published on Oct. 8, 2002)

[Patent Publication 3]

Japanese Laid-Open Patent Publication No. 2003-111593 (published on Apr. 15, 2003)

[Non-Patent Publication 1]

Jobs M, Howell W M, Stromqvist L, Mayr T, Brookes A J. Related Articles, Links. DASH-2: flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays. Genome Res. 2003 May; 13(5): 916-24.

[Non-Patent Publication 2]

Matsuzaki H, Loi H, Dong S, Tsai Y Y, Fang J, Law J, Di X, Liu W M, Yang G, Liu G, Huang J, Kennedy G C, Ryder T B, Marcus G A, Walsh P S, Shriver M D, Puck J M, Jones K W, Mei R. Links. Parallel Genotyping of Over 10,000 SNPs Using a One-Primer Assay on a High-Density Oligonucleotide Array. Genome Res. 2004 March; 14(3): 414-25.

As described above, genomes of many organisms have been sequenced and many type of arrays have been marketed. However, none of these arrays is usable for the breeding of Triticeae species. Meanwhile, while breeding using genetic markers can greatly improve efficiency as compared with the conventional screening conducted in a field, the genotype of each genetic marker needs to be confirmed individually. In breeding, large numbers of agriculturally desirable traits are screened for and undesirable traits are selected out. If genotypes of these multiple genetic markers were confirmed comprehensively, it would be possible to further improve efficiency of breeding.

The present invention was made in view of the foregoing problems, and an object of the invention is to provide a detection instrument for detecting, in Triticeae species, gene expression, gene polymorphism, proteins (polypeptides), and substances that interact with proteins (polypeptides).

DISCLOSURE OF INVENTION

In order to achieve the foregoing object, the inventors of the present invention designed primers based on the EST sequences independently developed by the inventors. By finding polymorphisms between different varieties in the amplified fragments that had been amplified using the genomic DNA as a template, genetic markers were developed. The genetic markers were mapped on barley chromosomes, and a detailed genetic map was made. Upon further study, the inventors have found that, if polynucleotides with the barley EST sequences or genetic marker sequences were immobilized on a support, it would be possible to realize a gene expression detection instrument or gene polymorphism detection instrument applicable to breeding of Triticeae species. Further, the inventors also found that a protein (polypeptide)-interacting substance detection instrument or protein (polypeptide) detection instrument could be realized when proteins encoded by the EST sequences, or antibodies against such proteins were immobilized on a support. The present invention was accomplished based on these findings.

Specifically, the present invention provides a gene detection instrument for detecting expression or polymorphism of genes existing in a genome of Triticeae species, the gene detection instrument comprising a support on which is immobilized at least one polynucleotide selected from: (a) polynucleotides with partial base sequences of chromosomal DNA of barley, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the partial base sequences of chromosomal DNA of barley; or (b) polynucleotides with combined partial base sequences of chromosomal DNA of barley, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the combined partial base sequences of chromosomal DNA of barley.

It is preferable that the polynucleotide immobilized on the support comprise at least one kind of polynucleotide selected from the group consisting of: (1) polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the base sequences of SEQ ID NO: 1 through 5780; (2) a polynucleotide that comprises a part of any one of the polynucleotides set forth in (1); (3) a polynucleotide whose partial sequence comprises all of or part of any one of the polynucleotides set forth in (1); and (4) a polynucleotide whose partial sequence comprises: all of or part of a base sequence of SEQ ID NO: n (where n is an odd number), or its variant base sequence, in the polynucleotides set forth in (1); and all of or part of a base sequence of SEQ ID NO: n+1, or its variant base sequence, in the polynucleotides set forth in (1). Since the polynucleotides include base sequence of barley cDNA, gene expression or gene polymorphism in Triticeae species can be detected with a gene detection instrument in which the polynucleotides are immobilized on a support.

It is preferable that two or more kinds of polynucleotides be immobilized on the support, and that regions on the support in which the polynucleotides are respectively immobilized be arranged in the same order as a chromosomal order of the polynucleotides immobilized on the support. The gene detection instrument may be adapted so that two or more kinds of polynucleotides are immobilized on the support, and that information indicative of a chromosomal order of the polynucleotides immobilized on the support is appended to regions on the support in which the polynucleotides are respectively immobilized. With the immobilizing regions arranged in the chromosomal order or with the information indicative of the chromosomal order, the locations of recombination that has occurred in crossbreeding of Triticeae species can be found with ease. As a result, efficiency of breeding can be improved.

It is preferable that the polynucleotide immobilized on the support comprise cDNA. When the polynucleotide is cDNA, gene expression can be evaluated efficiently through hybridization with polynucleotides in a sample.

According to the present invention, there is provided a gene polymorphism detection instrument for detecting polymorphism of genes existing in a genome of Triticeae species, the gene polymorphism detection instrument comprising a support on which is immobilized at least one polynucleotide selected from: polynucleotides with partial base sequences of chromosomal DNA of barley; or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the partial base sequences of chromosomal DNA of barley.

It is preferable that the polynucleotide immobilized on the support comprise a partial base sequence of at least one of DNA fragments amplified, using genomic DNA of Triticeae species as a template, with a primer set that comprises a combination of any two primers arbitrarily selected from: a plurality of primers designed based on a base sequence of SEQ ID NO: n (where n is an odd number) from among base sequences of SEQ ID NO: 1 through 5780; and a plurality of primers designed based on a base sequence of SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1 through 5780. Gene polymorphism in Triticeae species can be detected with the gene polymorphism detection instrument in which a polynucleotide with a polymorphism-containing base sequence of the amplified DNA fragments is immobilized on a support.

It is preferable that two or more kinds of polynucleotides be immobilized on the support, and that regions on the support in which the polynucleotides are respectively immobilized be arranged in the same order as a chromosomal order of the polynucleotides immobilized on the support. The gene polymorphism detection instrument may be adapted so that two or more kinds of polynucleotides are immobilized on the support, and that information indicative of a chromosomal order of the polynucleotides immobilized on the support is appended to regions on the support in which the polynucleotides are respectively immobilized. With the immobilizing regions arranged in the chromosomal order or with the information indicative of the chromosomal order, the locations of recombination that has occurred in crossbreeding of Triticeae species can be found with ease. As a result, efficiency of breeding can be improved.

It is preferable that the polynucleotide immobilized on the support comprise a synthetic oligonucleotide. With an oligonucleotide synthesized to have a sequence suitable for detection of polymorphism, the efficiency of detection can be improved.

According to the present invention, there is provided a polypeptide-interacting substance detection instrument for detecting a substance which interacts with a polypeptide that comprises a protein, or part of a protein, encoded by a gene present in the genome of Triticeae species, the polypeptide-interacting substance detection instrument comprising a support on which is immobilized at least one of polypeptides encoded by: (a) polynucleotides with partial base sequences of chromosomal DNA of barley, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the partial base sequences of chromosomal DNA of barley; or (b) polynucleotides with combined partial base sequences of chromosomal DNA of barley, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the combined partial base sequences of chromosomal DNA of barley.

It is preferable that the polypeptide immobilized on the support be encoded by a polynucleotide selected from the group consisting of: (1) polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the base sequences of SEQ ID NO: 1 through 5780; (2) a polynucleotide that comprises a part of any one of the polynucleotides set forth in (1); (3) a polynucleotide whose partial sequence comprises all of or part of any one of the polynucleotides set forth in (1); and (4) a polynucleotide whose partial sequence comprises: all of or part of a base sequence of SEQ ID NO: n (where n is an odd number), or its variant base sequence, in the polynucleotides set forth in (1); and all of or part of a base sequence of SEQ ID NO: n+1, or its variant base sequence, in the polynucleotides set forth in (1). Substances that interact with proteins of Triticeae species can be detected with the polypeptide-interacting substance detection instrument in which the polypeptides are immobilized on a support.

It is preferable that two or more kinds of polypeptides are immobilized on the support, and that regions on the support in which the polypeptides are respectively immobilized are arranged in the same order as a chromosomal order of the polynucleotides respectively encoding the polypeptides immobilized on the support. The polypeptide-interacting substance detection instrument may be adapted so that two or more kinds of polypeptides are immobilized on the support, and that information indicative of a chromosomal order of the polynucleotides respectively encoding the polypeptides immobilized on the support is appended to regions on the support in which the polypeptides are respectively immobilized. With the immobilizing regions arranged in the chromosomal order or with the information indicative of the chromosomal order, the locations of recombination that has occurred in crossbreeding of Triticeae species can be found with ease. As a result, efficiency of breeding can be improved.

According to the present invention, there is provided a polypeptide detection instrument for detecting a polypeptide that comprises a protein, or part of a protein, encoded by a gene present in a genome of Triticeae species, the polypeptide detection instrument comprising a support on which is immobilized at least one of antibodies against polypeptides encoded by: (a) polynucleotides with partial base sequences of chromosomal DNA of barley, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the partial base sequences of chromosomal DNA of barley; or (b) polynucleotides with combined partial base sequences of chromosomal DNA of barley, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the combined partial base sequences of chromosomal DNA of barley.

It is preferable that the polynucleotide encoding the polypeptide used for production of the antibody immobilized on the support comprise at least one kind of polynucleotide selected from the group consisting of: (1) polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, or polynucleotides mutated by substitution, deletion, insertion, and/or addition of one or more bases in the base sequences of SEQ ID NO: 1 through 5780; (2) a polynucleotide that comprises a part of any one of the polynucleotides set forth in (1); (3) a polynucleotide whose partial sequence comprises all of or part of any one of the polynucleotides set forth in (1); and (4) a polynucleotide whose partial sequence comprises: all of or part of a base sequence of SEQ ID NO: n (where n is an odd number), or its variant base sequence, in the polynucleotides set forth in (1); and all of or part of a base sequence of SEQ ID NO: n+1, or its variant base sequence, in the polynucleotides set forth in (1). Substances that interact with proteins of Triticeae species can be detected with the polypeptide-interacting substance detection instrument in which the antibodies are immobilized on a support.

It is preferable that two or more kinds of antibodies be immobilized on the support, and that regions on the support in which the antibodies are respectively immobilized be arranged in the same order as a chromosomal order of the polynucleotides respectively encoding polypeptides used for production of the antibodies immobilized on the support. The polypeptide detection instrument may be adapted so that two or more kinds of antibodies are immobilized on the support, and that information indicative of a chromosomal order of the polynucleotides respectively encoding polypeptides used for preparation of the antibodies immobilized on the support is appended to regions on the support in which the antibodies are respectively immobilized. With the immobilizing regions arranged in the chromosomal order or with the information indicative of the chromosomal order, the locations of recombination that has occurred in crossbreeding of Triticeae species can be found with ease. As a result, efficiency of breeding can be improved.

According to the present invention, there are provided polynucleotides usable for an instrument for detecting expression or polymorphism of genes present in the genome of Triticeae species, the polynucleotides comprising: base sequences of SEQ ID NO: 1 through 5780; or base sequences of SEQ ID NO: 1 through 5780, with substitution, deletion, insertion, and/or addition of one or more bases. Further, according to the present invention, there are provided polynucleotides whose partial sequence comprises polynucleotides usable for an instrument for detecting polymorphism of genes present in the genome of Triticeae species, the polynucleotides comprising DNA fragments amplified, using genomic DNA of Triticeae species as a template, with a primer set that comprises a combination of any two primers arbitrarily selected from: a plurality of primers designed based on a base sequence of SEQ ID NO: n (where n is an odd number) from among base sequences of SEQ ID NO: 1 through 5780; and a plurality of primers designed based on a base sequence of SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1 through 5780.

The foregoing polynucleotides are suitable as the polynucleotides immobilized on a support of a gene detection instrument and a gene polymorphism detection instrument according to the present invention.

Additional objects, features, and strengths of the present invention will be made clear by the description below. Further, the advantages of the present invention will be evident from the following explanation in reference to the drawings.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1( a) is a plan view of an array as one example of a detection instrument according to the present invention, schematically illustrating expression of genes conferring certain characteristics.

FIG. 1( b) is a plan view of an array as one example of a detection instrument according to the present invention, schematically illustrating expression of genes conferring characteristics different from the characteristics represented in FIG. 1( a).

FIG. 2 is a schematic view illustrating expression of genes conferring certain characteristics in a segregating population obtained from a cross between varieties that showed the gene expression represented in FIGS. 1( a) and 1(b), and in specific varieties selected from the segregating population.

FIG. 3 is a view showing a result of analysis on genotypes (recombination sites) of barley hybrids that were subjected to PCR in which polynucleotides immobilized on a support of a gene polymorphism detection instrument according to the present invention were used as primers, and genomic DNA of the barley hybrids was used as a template.

BEST MODE FOR CARRYING OUT THE INVENTION

The following will describe one embodiment of the present invention. It should be appreciated that the invention is not limited in any way by the following description.

(1) Gene Detection Instrument according to the Present Invention

A gene detection instrument according to the present invention is an instrument for detecting expression or polymorphism of genes in the genomes of Triticeae species. The organisms to which a gene detection instrument of the invention is applicable may be any Triticeae species, among which barley, wheat, and rye are preferable. As will be described later, a gene detection instrument according to the present invention includes a support on which polynucleotides constituting part of barley chromosomal (1H, 2H, 3H, 4H, 5H, 6H, and 7H) DNA are immobilized. The polynucleotides immobilized on the support may solely be polynucleotides that constitute part of the barley chromosomal DNA, or other polynucleotides may additionally be immobilized on the support. Such additional polynucleotides are not particularly limited as long as they can detect expression or polymorphism of genes in the genomes of Triticeae species. For example, the additional polynucleotides may be those with the base sequences originating in non-barley organisms, or those with arbitrary base sequences that have been artificially synthesized.

In the case where the polynucleotides are immobilized in more than one region of the support, the polynucleotides immobilized in these regions may have non-overlapping base sequences or partially overlapping base sequences. Alternatively, polynucleotides with the same base sequence may be immobilized in these different regions of the support. In the case where the polynucleotides have overlapping base sequences, the polynucleotides may have partially overlapping base sequences, or the base sequence of one of the polynucleotides may be a partial sequence of the other polynucleotide.

Further, the polynucleotide immobilized in each region is not necessarily required to be of the same kind. More than one kind of polynucleotide may be immobilized in each region.

The support is not particularly limited as long as it can immobilize polynucleotides, and it may have any shape and may be made of any material. Examples of a support material generally include: inorganic materials such as glass and silicon wafer; natural polymers such as paper; synthetic polymers such as nitrocellulose and nylon; and gels using synthetic polymers or natural polymers. The shape of the support is not particularly limited as long as it provides enough area to support the polynucleotides. Generally, those with a two-dimensional plane, for example, such as a substrate with little or no flexibility, a flexible membrane, or a flexible substrate with intermediate flexibility can be preferably used. The thickness of the substrate or membrane is not particularly limited either, and it can be suitably set according to the material or use of the substrate or membrane. Various types of beads may be used as supports.

[Polynucleotides Immobilized on a Support of the Gene Detection Instrument]

In a gene detection instrument according to the present invention, at least one polynucleotide from the following polynucleotides (a) or (b) is immobilized on a support.

(a) Polynucleotides with base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

(b) Polynucleotides with a combination of base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

As used herein, a polynucleotide with a base sequence constituting part of barley chromosomal DNA is not particularly limited as long as it is a polynucleotide with a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes. Further, a polynucleotide with a combination of base sequences constituting part of barley chromosomal DNA refers to a polynucleotide in which a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes is joined to non-continuous base sequences constituting other parts of the chromosomes. For example, base sequences from two different parts of the chromosomes may constitute the polynucleotide, or three or more base sequences may join together to form the polynucleotide. Specifically, for example, cDNA with a plurality of exons from a protein-coding gene on barley chromosomal DNA can be regarded as a polynucleotide with a combination of base sequences constituting part of barley chromosomal DNA. However, the polynucleotide is not just limited to this specific example.

A variant with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotide with a base sequence, or a combination of base sequences, constituting part of barley chromosomal DNA may be a polynucleotide that has been mutated on purpose, or a polynucleotide that exists in nature. For example, think of a base sequence of chromosomal DNA in a specific variety of barley. Comparing this base sequence with those of other varieties, no sequence is completely identical. Rather, these sequences are variants with the substitution, deletion, insertion, and/or addition of one or more bases.

Polynucleotides immobilized on a support of a gene detection instrument according to the present invention are preferably polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, or variants with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotides with the base sequences of SEQ ID NO: 1 through 5780. (Such polynucleotides and variants will be referred to as polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780.)

The base sequences of SEQ ID NO: 1 through 5780 are base sequences of the barley EST (expressed sequence tag) independently developed by the inventors. The inventors have previously confirmed that a polynucleotide with the base sequences of SEQ ID NO: 1 through 770, a polynucleotide with the base sequences of SEQ ID NO: 771 through 1754, a polynucleotide with the base sequences of SEQ ID NO: 1755 through 2642, a polynucleotide with the base sequences of SEQ ID NO: 2643 through 3324, a polynucleotide with the base sequences of SEQ ID NO: 3325 through 4320, a polynucleotide with the base sequences of SEQ ID NO: 4321 through 4962, and a polynucleotide with the base sequences of SEQ ID NO: 4963 through 5780 are mapped on 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes, respectively.

The following briefly describes the inventors' EST and mapping. The inventors prepared mRNA from leaves of several different varieties of barley, and constructed cDNA libraries according to a conventional method. Plasmids with the cDNA were used as templates, and the base sequences in portions of the plasmids on the both ends of the inserts were used as primers. The base sequence that was read by a single sequence analysis on either end was adjusted, and a vector sequence was removed therefrom to obtain the barley EST sequence. Currently, about 140,000 EST sequences are available from the database of the inventors.

These EST sequences were divided into groups of about 10,000 genes based on homology of sequences at the 3′ end. Using primer design software Primer 3, a primer set was designed for each EST, starting from the EST with the longest sequence. These primer sets were used to check for polymorphism in the hybrids of the mapped populations: malting barley Haruna Nijo and wild type barley H602. More specifically, first, the fragment lengths yielded by agarose gel electrophoresis were checked for polymorphism. If there were no difference in fragment length, the amplified fragments of the parents were aligned by direct sequencing. If the base sequences of the parents had differences to be recognized by restriction enzyme, the amplified fragments were digested with restriction enzyme and subjected to agarose gel electrophoresis to detect differences in fragment length. Then, using MAPMAKER/EXP, a linkage analysis was performed using the marker polymorphism information, together with the markers with known chromosomal locations. In this way, a genetic map based on the EST sequences was constructed.

It follows from this that the base sequences of SEQ ID NO: 1 through 5780 are partial sequences of barley cDNA. Thus, if polynucleotides with the base sequences of SEQ ID NO: 1 through 5780 were immobilized on the support, gene expression can be detected through hybridization with mRNA (probe based on mRNA) in a sample. Variants with the substitution, deletion, insertion, and/or addition of one or more bases in the base sequences of SEQ ID NO: 1 through 5780 may be polynucleotides that have been mutated on purpose, or polynucleotides that exist in nature.

A polynucleotide immobilized on a support of a gene detection instrument according to the present invention may be a part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780. Since the base sequence of such partial polynucleotide is a partial base sequence of barley cDNA, it can still be used to detect gene expression.

Further, a polynucleotide immobilized on a support of a gene detection instrument according to the present invention may be a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. The remaining base sequences of the polynucleotide are not limited. For example, since the base sequences of SEQ ID NO: 1 through 5780 are partial sequences of barley cDNA, these base sequences do not have the sequences on either end as originally found in the full length cDNA. Thus, a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780, and which additionally includes the cDNA sequences on the both ends or one end as originally found in the full length cDNA can be regarded as a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. Further, vectors such as plasmids and BACs (bacterial artificial chromosomes) that have incorporated all of or part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780, and polynucleotides in which the partial sequence is ligated to arbitrary base sequences can also be regarded as polynucleotides whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. Such polynucleotides at least include all of or part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780, i.e., part of barley cDNA, and are therefore capable of detecting gene expression.

Further, polynucleotides immobilized on a support of a gene detection instrument according to the present invention may be polynucleotides or the like whose partial sequences comprise all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n (where n is an odd number), and all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780. As described above, the base sequences of SEQ ID NO: 1 through 5780 are EST sequences of barley, and comprise sequences that can be read by sequencing the cloned cDNA from the both ends only once. In other words, the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 among the base sequences of SEQ ID NO: 1 through 5780 are base sequences that are read from the both ends of the cDNA of the same clone. As such, these base sequences can realize a full length cDNA base sequence, which corresponds to all of or part of the full length cDNA. Thus, polynucleotides or the like whose partial sequences comprise all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n (where n is an odd number), and all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780 can be regarded as polynucleotides with full length cDNA that comprises the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1, or polynucleotides that comprise part of the full length cDNA. Further, the foregoing polynucleotides may be polynucleotides in which vector sequences or arbitrary base sequences for example are ligated to the both ends or one end of the full length cDNA or polynucleotides that comprise part of the full length cDNA. Further, the foregoing polynucleotides may be variants that have a base substitution or other mutations in sequences other than the base sequences of SEQ ID NO: 1 through 5780, i.e., a middle section of the total cDNA unspecified by SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1. Such polynucleotides include the full length or part of barley cDNA, and are therefore capable of detecting gene expression.

The polynucleotide immobilized on a support is preferably cDNA. In this case, cDNA is not limited to full length cDNA. Rather, it may be a polynucleotide that comprises part of cDNA. Further, the polynucleotide may include other base sequences, for example, such as vector sequences, as long as it includes cDNA. Further, the polynucleotide may be a synthetic oligonucleotide that is produced by artificially synthesizing part of the cDNA base sequences.

In the case where a gene detection instrument according to the present invention is used for detection of gene expression, the substance to be detected in a sample is mRNA (probe based on mRNA). Therefore, for strong hybridization, it is preferable that the polynucleotide immobilized on a support be full length cDNA. The polynucleotide immobilized on a support may include any number of bases as long as it can detect gene expression. For example, in the case where only one polynucleotide is immobilized in each region, a polynucleotide with at least 50 bases is considered to be sufficient for detection of gene expression. When more than one polynucleotide (oligonucleotide) is immobilized in each region as in the Affymetrix system, a polynucleotide with about 25 bases is sufficient.

[Gene Detection Instrument with the Polynucleotides Immobilized in Regions that are Arranged in the Chromosomal Order]

As described above, the base sequences of SEQ ID NO: 1 through 5780 are base sequences of barley EST mapped on barley chromosomes. Further, the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 are base sequences that are read from the both ends of cDNA of the same clone, wherein n represents a base sequence on the 5′ end, and n+1 represents a base sequence on the 3′ end.

As used herein, the “chromosomal order” refers to the order from an arbitrary position of a chromosome. The distance by which the order is determined may be a genetic distance based on recombinations in hybrid populations, or a physical distance based on the number of bases or the length of chromosomes observed with a microscope.

As shown in Table 1-1 to Table 1-8, the chromosomal order in barley 1H chromosome (distance from the short arm end of 1H chromosome) has been specified for 385 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1 through SEQ ID NO: 770. The chromosomal order in barley 1H chromosome has also been specified for 3 known clones (HVM20, Bmag211, and WMCIE8).

TABLE 1-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 bah55e06 1 2 0 2 bags30b07 3 4 14 3 baak2103 5 6 17.4 4 BaAK17D13 7 8 18.5 4 basd26120 9 10 18.5 4 bah61p17 11 12 18.5 7 BaGS11O06 13 14 19.6 7 kr12H0216 15 16 19.6 7 BaGS32E23 17 18 19.6 7 basd13k20 19 20 19.6 11 BaAK24O11 21 22 20.7 11 kr26D0507 23 24 20.7 11 BaSD2D08 25 26 20.7 11 baak41n21 27 28 20.7 15 bah11b15 29 30 21.8 15 kr24B0903 31 32 21.8 17 bast50E0709 33 34 22.9 18 baal17o01 35 36 23.5 19 baak41a04 37 38 24 20 BaH28C07 39 40 24.2 21 bags16g18 41 42 24.4 22 BaSD3C22 43 44 25.1 23 BaGS17B21 45 46 26.7 23 basd27b10 47 48 26.7 25 bah47d23 49 50 27.2 26 BaAK21D02 51 52 27.7 27 BaH17D02 53 54 32.1 28 baal4f12 55 56 32.7 29 BaGS11I03 57 58 33.8 29 bah56a03 59 60 33.8 29 kr16A0501 61 62 33.8 29 bah19f01 63 64 33.8 33 BaAK27F07 65 66 37 34 bah47f18 67 68 41.1 34 bah45l19 69 70 41.1 36 BaAK12I12 71 72 44.1 37 BaAL6N04 73 74 45.2 37 baal16105 75 76 45.2 37 BaSD18O20 77 78 45.2 37 BaSD3J13 79 80 45.2 41 bah63j19 81 82 49.5 41 BaH36O18 83 84 49.5 43 BaAK20A06 85 86 50.6 43 BaGS8G13 87 88 50.6 45 bah25n06 89 90 53.8 45 BaAK1P06 91 92 53.8 47 BaAK16M07 93 94 55.1 47 BaH36M15 95 96 55.1 49 BaGS12K12 97 98 62.4 50 BaAL39C22 99 100 71.9

TABLE 1-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 50 kr68B0303 101 102 71.9 52 baak24d09 103 104 75.8 53 BaGS11J13 105 106 76.9 54 baal38l23 107 108 79.5 54 BaAL36B15 109 110 79.5 56 bast60D0610 111 112 80.6 56 kr22D0808 113 114 80.6 56 baal19i12 115 116 80.6 56 basd23o02 117 118 80.6 56 baak1i14 119 120 80.6 56 baak24k18 121 122 80.6 56 kr18G0814 123 124 80.6 63 BaH38H09 125 126 82.3 64 BaGS8B13 127 128 85.4 64 BaH25J08 129 130 85.4 66 baak3d11 131 132 88.6 67 baal12p08 133 134 89.7 67 bags39i20 135 136 89.7 67 bags32m16 137 138 89.7 70 kr15A0402 139 140 91.3 71 baal3c01 141 142 91.9 72 baak41i03 143 144 93.9 73 BaGS29M13 145 146 95.7 74 basdl7m22 147 148 98 74 bags15g01 149 150 98 74 BaH24I06 151 152 98 77 BaH57E12 153 154 99.1 78 BaSD25C22 155 156 99.7 79 baal19m17 157 158 101.9 80 bah46p14 159 160 102.3 80 bags18d19 161 162 102.3 80 BaAK34J19 163 164 102.3 83 bags6d01 165 166 105.6 84 bags3p11 167 168 108.9 84 BaAK14J21 169 170 108.9 84 baak2m05 171 172 108.9 87 baak37g19 173 174 109.4 88 BaGS31B11 175 176 109.9 88 BaAL35M08 177 178 109.9 88 BaSD16G03 179 180 109.9 88 BaH58J20 181 182 109.9 88 BaGS22B13 183 184 109.9 88 BaAK43M01 185 186 109.9 88 bah14i07 187 188 109.9 88 BaH13F14 189 190 109.9 88 baal9i16 191 192 109.9 88 basd27b20 193 194 109.9 88 BaAK2O24 195 196 109.9 88 basd18l16 197 198 109.9 88 BaAL19J14 199 200 109.9

TABLE 1-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 88 bags22i15 201 202 109.9 102 baak44a23 203 204 111.5 102 BaAK32N04 205 206 111.5 104 basd21e22 207 208 112 104 BaGS7K03 209 210 112 104 baak42e22 211 212 112 107 bast21D0808 213 214 113.1 107 bast75E0610 215 216 113.1 107 baet16A1002 217 218 113.1 107 basd17m16 219 220 113.1 107 bags17g08 221 222 113.1 107 baak21i01 223 224 113.1 107 baakl3n06 225 226 113.1 107 baak22o23 227 228 113.1 107 baet42E0410 229 230 113.1 116 BaGS27M04 231 232 114.2 116 BaH52H18 233 234 114.2 116 baak33k20 235 236 114.2 116 bags21f16 237 238 114.2 116 bags34e05 239 240 114.2 116 bags15d20 241 242 114.2 116 bags18g10 243 244 114.2 116 bags19a02 245 246 114.2 116 BaAK17J19 247 248 114.2 125 bags35k02 249 250 115.3 126 bah35a22 251 252 116.4 126 BaGS17H13 253 254 116.4 126 bah47n12 255 256 116.4 126 baak12p11 257 258 116.4 126 baet38B1004 259 260 116.4 126 BaGS13K12 261 262 116.4 126 BaAL25A05 263 264 116.4 133 bah27k23 265 266 117.2 134 BaAK24J12 267 268 118.3 135 BaSD14M22 269 270 119.8 135 bah17l24 271 272 119.8 137 baal29i09 273 274 120.5 137 bah16d09 275 276 120.5 137 bah60d03 277 278 120.5 137 bah45i13 279 280 120.5 137 bah11h03 281 282 120.5 137 bah47h17 283 284 120.5 143 bags35d02 285 286 122.7 143 bags37e17 287 288 122.7 143 baak14c12 289 290 122.7 143 baak34c01 291 292 122.7 143 BaH35A11 293 294 122.7 148 BaAK30M16 295 296 123.8 148 BaSD11P04 297 298 123.8 148 bah12i09 299 300 123.8

TABLE 1-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 148 BaAL2G20 301 302 123.8 152 bags14j09 303 304 126 152 kr26E0610 305 306 126 152 bah37f01 307 308 126 152 bags29m05 309 310 126 156 BaGS17A18 311 312 126.5 157 baet34A0501 313 314 127 157 BaSD22O13 315 316 127 157 bags15a22 317 318 127 157 baet21H1016 319 320 127 157 BaH30E13 321 322 127 157 bags4e23 323 324 127 157 baak45b03 325 326 127 157 bah12o12 327 328 127 165 baak34k14 329 330 127.9 166 baal3e14 331 332 131.5 167 bags22f12 333 334 136.3 168 BaH45P03 335 336 137.2 168 basd11d13 337 338 137.2 168 Bmag211 — — 137.2 168 HVM20 — — 137.2 168 basd1j14 339 340 137.2 173 bags35b18 341 342 138.3 173 baal41i11 343 344 138.3 175 bags13e23 345 346 139.4 176 bah20d03 347 348 140 177 baet19F0212 349 350 140.5 177 bags12j05 351 352 140.5 179 BaSD24D17 353 354 141.6 180 BaGS24K10 355 356 142.1 181 baal9e05 357 358 142.6 181 BaGS33M23 359 360 142.6 183 bah46g14 361 362 143.1 184 bags33h05 363 364 143.6 185 baak24h12 365 366 143.9 186 BaH50N19 367 368 144.2 187 BaAK31O05 369 370 144.4 188 BaGS39L14 371 372 144.7 189 BaGS27C22 373 374 146.8 189 bags20o24 375 376 146.8 189 bags34j05 377 378 146.8 189 bah56l03 379 380 146.8 189 basd12k03 381 382 146.8 189 BaAK39I18 383 384 146.8 189 bags21h06 385 386 146.8 189 bah56k04 387 388 146.8 189 bah60e11 389 390 146.8 198 baak22i05 391 392 147.9 198 baal5i02 393 394 147.9 200 BaAK2E05 395 396 149

TABLE 1-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 201 baak33g12 397 398 151.2 201 bags4e05 399 400 151.2 203 bast42C0806 401 402 155.4 204 baak26c05 403 404 156.6 205 bags19a16 405 406 158.6 206 kr11F1212 407 408 159.7 206 BaH28K13 409 410 159.7 206 baak20d06 411 412 159.7 206 BaSD1I24 413 414 159.7 206 baak35l20 415 416 159.7 211 bah57m07 417 418 161.8 211 bah60k11 419 420 161.8 213 basd20o09 421 422 162.9 213 basd24i22 423 424 162.9 215 bags30g20 425 426 164 216 bags1m11 427 428 164.6 217 bags23g20 429 430 165.1 218 BaGS15B05 431 432 166.2 219 BaGS24P05 433 434 167.3 220 bags10g06 435 436 168.4 220 bah32o04 437 438 168.4 220 BaGS32P08 439 440 168.4 223 bags4e02 441 442 169.5 223 bast04H0315 443 444 169.5 223 BaGS37L06 445 446 169.5 226 basd2b18 447 448 170.6 227 bags3h12 449 450 172.1 228 bags1e21 451 452 174.6 228 BaAK27M21 453 454 174.6 230 BaSD18F05 455 456 174.9 231 BaH35B05 457 458 175.3 232 basd21h11 459 460 176.4 232 BaH32E20 461 462 176.4 234 BaGS22A20 463 464 177.5 235 BaSD14B13 465 466 179 236 bags29l04 467 468 182 236 bags22g16 469 470 182 238 BaAL4B14 471 472 184.2 238 bah61h20 473 474 184.2 240 bah15p01 475 476 186.4 240 baak41p03 477 478 186.4 242 BaSD12L06 479 480 187.7 242 BaSD23P07 481 482 187.7 242 BaGS7J05 483 484 187.7 245 bah29b06 485 486 188.3 245 BaGS31N17 487 488 188.3 247 BaGS13F08 489 490 190.4 248 BaAK39G10 491 492 195.7 249 BaAK39G03 493 494 200 249 baak2a18 495 496 200

TABLE 1-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 251 bags39d12 497 498 201.1 251 basd17l04 499 500 201.1 251 BaAK20B19 501 502 201.1 251 bags15j15 503 504 201.1 251 BaAK30F02 505 506 201.1 256 BaGS22A21 507 508 202.2 256 baet45E0410 509 510 202.2 256 BaGS22C14 511 512 202.2 256 bah23k12 513 514 202.2 260 BaH15O13 515 516 203.2 260 kr24F0412 517 518 203.3 260 BaGS28O21 519 520 203.3 260 BaAL15N19 521 522 203.3 264 bags10j15 523 524 207.6 265 bags32j03 525 526 209.7 266 bags20e14 527 528 211.1 267 bags3c15 529 530 214 268 basd27n01 531 532 216 269 BaGS6M19 533 534 217.1 270 baak13e03 535 536 219.2 271 BaH15M10 537 538 221.5 271 bags14k12 539 540 221.5 271 bast61E0509 541 542 221.5 271 BaGS31N04 543 544 221.5 275 BaAK27D22 545 546 230 275 baal5o19 547 548 230 277 bah55b18 549 550 231.1 278 bah23i02 551 552 232.6 278 BaAK15H22 553 554 232.6 280 bags32b03 555 556 244.3 281 baak30c15 557 558 248.9 281 BaGS22A05 559 560 248.9 281 bah16m01 561 562 248.9 284 bags15e08 563 564 250 284 BaGS37D12 565 566 250 284 BaAL37N24 567 568 250 287 BaH28B09 569 570 251.1 287 bah47h08 571 572 251.1 289 BaH57N07 573 574 252.2 290 baak38o02 575 576 253.5 290 baak20f16 577 578 253.5 292 baak28n19 579 580 257.4 292 bah59j07 581 582 257.4 292 bah44j20 583 584 257.4 292 BaH47J05 585 586 257.4 296 baet39B0303 587 588 260.7 297 BaAK27D19 589 590 261.8 297 basd25g01 591 592 261.8 299 BaAL34K17 593 594 262.9 299 basd21i17 595 596 262.9

TABLE 1-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 301 bags15o12 597 598 264 301 baal2j10 599 600 264 303 baak44n10 601 602 264.4 304 baak21e08 603 604 264.8 305 BaGS37F14 605 606 265.2 306 BaAK41I21 607 608 266.3 306 BaGS19C07 609 610 266.3 308 BaGS15O08 611 612 268.4 308 baak40p22 613 614 268.4 310 BaH21K05 615 616 269.5 310 baal40n03 617 618 269.5 312 bags23b08 619 620 269.9 313 BaGS39L18 621 622 270.6 313 BaGS25K24 623 624 270.6 313 bags1a18 625 626 270.6 313 baal15k07 627 628 270.6 313 bags14o13 629 630 270.6 318 BaH18D15 631 632 271.7 319 bastl04F0911 633 634 272.8 319 BaGS31E03 635 636 272.8 321 BaGS19J21 637 638 273.9 322 BaGS39P08 639 640 275 323 BaH56B06 641 642 276.1 324 baal13m24 643 644 277.2 324 bags15h14 645 646 277.2 324 bags35j22 647 648 277.2 324 baak20h22 649 650 277.2 328 baal34b14 651 652 278.3 328 bah16j04 653 654 278.3 328 BaH54J03 655 656 278.3 331 bast58C1206 657 658 280.4 332 BaH26M05 659 660 281.5 332 bastl20B0404 661 662 281.5 334 baak14e23 663 664 288.1 334 BaH15P22 665 666 288.1 334 BaGS17I22 667 668 288.1 337 bags15h01 669 670 292.4 338 bags7p13 671 672 294.5 339 BaGS29H13 673 674 296.7 339 bah47b01 675 676 296.7 341 bastl45E1109 677 678 299.9 341 bags32m15 679 680 299.9 341 basd12c09 681 682 299.9 344 baal27m11 683 684 301 344 basd12k01 685 686 301 344 baak41n15 687 688 301 344 bags1b01 689 690 301 348 BaH56O11 691 692 302.1 349 bah15k16 693 694 303.2 349 bags31a22 695 696 303.2

TABLE 1-8 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 349 bags21e21 697 698 303.2 349 baak32n05 699 700 303.2 353 baet02B0503 701 702 304.3 353 BaH29L05 703 704 304.3 355 baet43H1016 705 706 305.4 356 BaGS38N20 707 708 306.5 356 BaGS23O09 709 710 306.5 356 bastl26B0410 711 712 306.5 359 BaH15N14 713 714 309.6 359 baak20b06 715 716 309.6 361 BaH16I04 717 718 311.7 362 bah22p07 719 720 312.8 363 baak21j02 721 722 313.9 363 bah30o13 723 724 313.9 363 bags38f18 725 726 313.9 363 bah13o05 727 728 313.9 363 baak36b12 729 730 313.9 363 bags18o09 731 732 313.9 363 BaAK16L10 733 734 313.9 363 BaAK38E16 735 736 313.9 371 bah13e15 737 738 317.4 372 bags1f22 739 740 319.8 373 bags21o12 741 742 321.4 374 BaGS9B14 743 744 324.7 375 BaAL1N23 745 746 325.2 375 bbak1a17 747 748 325.2 377 BaH32B01 749 750 326.3 377 BaSD18L13 751 752 326.3 379 baak12p07 753 754 328.4 379 BaH58A04 755 756 328.4 381 bags1p04 757 758 329.1 381 BaAL17O03 759 760 329.1 383 baal8e17 761 762 332 384 BaH50I05 763 764 335.2 384 bastl28A0101 765 766 335.2 386 BaH39L18 767 768 336.3 387 bags18e18 769 770 342.1 388 WMCIE8 — — 362.7

As shown in Table 2-1 to Table 2-10, the chromosomal order in barley 2H chromosome (distance from the short arm end of 2H chromosome) has been specified for 492 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 771 through SEQ ID NO: 1754. The chromosomal order in barley 2H chromosome has also been specified for 8 known clones (Bmac134, cMWG682, HVM36, cMWG699, Bmag125, cMWG694, EBmac415, and MWG2076).

TABLE 2-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 BaGS20L10 771 772 0 2 BaSD15P22 773 774 15.4 2 bags7b16 775 776 15.4 4 BaAK34H02 777 778 20.7 5 BaAK22H13 779 780 21.7 6 BaGS37P19 781 782 23.2 7 BaAL19P17 783 784 24.3 8 BaH41L14 785 786 25.2 9 BaAK24H17 787 788 26.3 10 bast21A0602 789 790 26.4 10 BaAL27L20 791 792 26.4 12 Bmac134 — — 26.7 12 cMWG682 — — 26.7 12 bags34p10 793 794 26.7 12 basd18b14 795 796 26.7 16 bags38p20 797 798 27.8 17 BaAK41N22 799 800 28.9 17 BaAK21D17 801 802 28.9 19 BaAL29B07 803 804 30 19 baak20o16 805 806 30 21 bast42A0602 807 808 31.1 21 BaH36B07 809 810 31.1 21 BaAK26L07 811 812 31.1 21 baal12a06 813 814 31.1 25 BaSD3C20 815 816 33.3 26 bastl17G0113 817 818 34.6 27 baak11h14 819 820 39.2 28 baal12m14 821 822 40.2 29 baal33a18 823 824 41.3 30 bah28a18 825 826 43.5 30 bags39o04 827 828 43.5 30 BaH48H04 829 830 43.5 30 BaGS6B11 831 832 43.5 34 bags4p16 833 834 44.6 34 basd24j22 835 836 44.6 34 basd16p15 837 838 44.6 34 bags15k16 839 840 44.6 34 bast23D1208 841 842 44.6 39 BaAL32B22 843 844 45.3 39 BaGS39D07 845 846 45.3 41 BaH19L09 847 848 48 41 bags38a17 849 850 48 41 BaAK39I11 851 852 48 44 BaSD3I24 853 854 49.9 44 BaH35F01 855 856 49.9 46 BaAL30K02 857 858 53.9 47 bah13l23 859 860 54.9 48 BaAL26H21 861 862 55.7 49 BaGS22H22 863 864 56.8 49 bags13n11 865 866 56.8

TABLE 2-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 51 bags1h03 867 868 62 52 baak32l16 869 870 63.2 52 baal19a12 871 872 63.2 52 BaAK45G16 873 874 63.2 55 BaGS37N19 875 876 64.3 56 baak3f03 877 878 65.4 57 bags5c02 879 880 66.4 58 BaGS35P07 881 882 67.4 58 HVM36 — — 67.4 60 bah45e07 883 884 70.5 61 BaAK24B09 885 886 71.6 61 bags33a11 887 888 71.6 63 bah62i11 889 890 72.7 64 BaH56A24 891 892 74.9 65 BaSD21D14 893 894 77.1 66 basd1a17 895 896 78.2 66 bah17n24 897 898 78.2 68 kr70G0113 899 900 79.3 68 basd15f08 901 902 79.3 68 BaGS4J04 903 904 79.3 68 baak33f06 905 906 79.3 72 basd14f16 907 908 79.9 73 BaH59K20 909 910 80.4 73 baal35h05 911 912 80.4 73 BaGS20M01 913 914 80.4 76 BaH25N22 915 916 82.6 77 baak14a24 917 918 83.7 77 baak30d07 919 920 83.7 79 bah11n18 921 922 84.8 79 kr14C0305 923 924 84.8 79 baet18F0911 925 926 84.8 79 BaGS4J18 927 928 84.8 83 bast74C0705 929 930 85.9 83 BaAL4G17 931 932 85.9 85 bastl43H0515 933 934 86.2 85 BaH58M22 935 936 86.2 87 bags10i21 937 938 87 87 bah20h16 939 940 87 87 BaH17P13 941 942 87 87 baak41d10 943 944 87 91 BaGS14F01 945 946 88.1 91 BaAK16E24 947 948 88.1 93 BaSD18F09 949 950 92.4 93 baet42A0501 951 952 92.4 93 BaAK20L07 953 954 92.4 96 baak16e20 955 956 92.9 97 BaGS13N14 957 958 93.5 98 basd13m14 959 960 94.6 99 bags20g23 961 962 95.2 100 basd1l17 963 964 95.7

TABLE 2-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 100 basd26l01 965 966 95.7 100 BaAL37J18 967 968 95.7 103 bags7i05 969 970 96.8 103 bah60m17 971 972 96.8 105 bags21l01 973 974 99 106 baet31E0109 975 976 101.2 107 bags34m12 977 978 102.3 107 bah50n02 979 980 102.3 107 BaH50M11 981 982 102.3 107 bast27E1010 983 984 102.3 107 bags23c12 985 986 102.3 107 bah51m11 987 988 102.3 113 baak20d17 989 990 102.8 114 bags23l21 991 992 103.4 115 BaH52K04 993 994 104.5 115 BaAL37F24 995 996 104.5 115 bags23d01 997 998 104.5 118 BaSD11K22 999 1000 105 119 baal29m02 1001 1002 105.5 119 bah17e21 1003 1004 105.5 119 bah56j18 1005 1006 105.5 119 bags38n06 1007 1008 105.5 119 baet44D1208 1009 1010 105.5 119 BaAL34O13 1011 1012 105.5 125 BaGS4N05 1013 1014 108.7 125 BaSD15P20 1015 1016 108.7 125 baal13d11 1017 1018 108.7 125 bah27g02 1019 1020 108.7 125 basd27m10 1021 1022 108.7 125 basd23f16 1023 1024 108.7 125 bah16i19 1025 1026 108.7 125 BaH50I20 1027 1028 108.7 125 BaH34M23 1029 1030 108.7 134 bah28b24 1031 1032 109.3 135 BaH50P13 1033 1034 109.8 135 basd11m16 1035 1036 109.8 137 bags10p15 1037 1038 110.9 137 bags4g01 1039 1040 110.9 137 bags10k08 1041 1042 110.9 137 bags5e16 1043 1044 110.9 141 bags18l02 1045 1046 114.1 141 baak44k02 1047 1048 114.1 141 bags35a20 1049 1050 114.1 141 BaGS26M11 1051 1052 114.1 141 basd14h21 1053 1054 114.1 141 BaSD13D12 1055 1056 114.1 147 bags38k23 1057 1058 114.3 147 BaH42E05 1059 1060 114.3 147 bastl55A0701 1061 1062 114.3 147 bags13g18 1063 1064 114.3

TABLE 2-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 147 BaH15D23 1065 1066 114.3 147 kr28B0604 1067 1068 114.3 147 bags10f01 1069 1070 114.3 154 BaGS33I07 1071 1072 116.4 154 BaSD27B02 1073 1074 116.4 154 BaGS30N12 1075 1076 116.4 154 basd3h13 1077 1078 116.4 154 BaGS37L19 1079 1080 116.4 159 bah16g18 1081 1082 117.5 159 bags20i15 1083 1084 117.5 161 bags35c23 1085 1086 118 162 bah56k07 1087 1088 118.5 162 bags37d02 1089 1090 118.5 164 BaAK19K05 1091 1092 121.6 164 bah11i16 1093 1094 121.6 166 bags22l23 1095 1095 123.6 166 kr33A0901 1097 1098 123.6 168 bastl38C0606 1099 1100 125.4 168 baak11i13 1101 1102 125.4 168 BaH58E19 1103 1104 125.4 168 BaGS31G22 1105 1106 125.4 168 bah37h01 1107 1108 125.4 168 BaAL29P13 1109 1110 125.4 174 bags39a22 1111 1112 126 175 bah19g10 1113 1114 126.4 175 BaAL31A14 1115 1116 126.4 175 BaH50G15 1117 1118 126.4 175 BaH51M12 1119 1120 126.4 175 bags18k22 1121 1122 126.4 175 BaGS20N21 1123 1124 126.4 175 BaSD17O21 1125 1126 126.4 175 BaH37G17 1127 1128 126.4 183 bags30l22 1129 1130 128.6 184 baak22b17 1131 1132 129.7 184 bags20f22 1133 1134 129.7 186 bast72G0113 1135 1136 131.8 186 BaH30B05 1137 1138 131.8 186 BaH60B14 1139 1140 131.8 186 BaH17B16 1141 1142 131.8 190 basd12n23 1143 1144 133.9 190 BaH61A21 1145 1146 133.9 190 BaAK19H17 1147 1148 133.9 190 bah47l12 1149 1150 133.9 190 baak18p11 1151 1152 133.9 190 baet29H0715 1153 1154 133.9 190 BaH13D11 1155 1156 133.9 190 baet39D1107 1157 1158 133.9 190 BaH53E15 1159 1160 133.9 190 bags32o15 1161 1162 133.9 190 bast48A0701 1163 1164 133.9

TABLE 2-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 190 bast62D0907 1165 1166 133.9 190 BaH45P22 1167 1168 133.9 190 BaGS32L16 1169 1170 133.9 190 BaAK30J05 1171 1172 133.9 190 bah52a14 1173 1174 133.9 190 bah58i22 1175 1176 133.9 190 BaH56L16 1177 1178 133.9 190 bah52a21 1179 1180 133.9 190 BaSD14G11 1181 1182 133.9 190 bags6a03 1183 1184 133.9 190 bast72E0109 1185 1186 133.9 190 bags21a21 1187 1188 133.9 190 BaH58P13 1189 1190 133.9 214 bah27n22 1191 1192 134.4 215 bags20c13 1193 1194 134.9 215 bags30i14 1195 1196 134.9 215 BaAK17E11 1197 1198 134.9 215 baal1d17 1199 1200 134.9 215 bags22f06 1201 1202 134.9 215 baal10l01 1203 1204 134.9 215 baak26e17 1205 1206 134.9 215 bah16a03 1207 1208 134.9 215 BaAL11F18 1209 1210 134.9 215 BaAK29E10 1211 1212 134.9 215 bags32d21 1213 1214 134.9 215 bah54j22 1215 1216 134.9 215 baak16f14 1217 1218 134.9 215 baak44c12 1219 1220 134.9 215 bah13f11 1221 1222 134.9 215 baal39m19 1223 1224 134.9 215 baak4e02 1225 1226 134.9 215 baak46f04 1227 1228 134.9 215 BaAK13N23 1229 1230 134.9 215 BaGS20E09 1231 1232 134.9 215 baal4f01 1233 1234 134.9 215 BaSD19I17 1235 1236 134.9 215 baal4l21 1237 1238 134.9 215 BaAK19P01 1239 1240 134.9 215 BaAK31O14 1241 1242 134.9 240 BaH26P22 1243 1244 135.3 241 baal13d17 1245 1246 136 242 bags22j12 1247 1248 138.3 242 bags33p05 1249 1250 138.3 242 bags38j07 1251 1252 138.3 242 kr59F0311 1253 1254 138.3 242 baal13e10 1255 1256 138.3 242 bags15d19 1257 1258 138.3 242 BaGS5K11 1259 1260 138.3 242 BaGS10J14 1261 1262 138.3 242 baak41m17 1263 1264 138.3

TABLE 2-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 242 bah57o03 1265 1266 138.3 242 baak41d22 1267 1268 138.3 242 bah61c16 1269 1270 138.3 242 BaAL7M13 1271 1272 138.3 242 BaAK31F11 1273 1274 138.3 242 basd22k05 1275 1276 138.3 242 basd3p19 1277 1278 138.3 242 baak17o09 1279 1280 138.3 242 baal33d23 1281 1282 138.3 242 BaAK12F04 1283 1284 138.3 242 bah53j16 1285 1286 138.3 262 BaSD2E24 1287 1288 138.5 263 bags39h08 1289 1290 138.7 264 BaSD1N02 1291 1292 139.5 264 bags20m21 1293 1294 139.5 264 baal10h19 1295 1296 139.5 264 basd12m11 1297 1298 139.5 264 bah29d24 1299 1300 139.5 264 BaGS39P09 1301 1302 139.5 264 BaGS31F17 1303 1304 139.5 264 BaGS23D08 1305 1306 139.5 264 bags39d15 1307 1308 139.5 264 BaGS34I17 1309 1310 139.5 274 BaH32N02 1311 1312 141.6 274 BaSD18H19 1313 1314 141.6 274 bast63A0101 1315 1316 141.6 274 BaH50N04 1317 1318 141.6 278 baal4a13 1319 1320 147.5 278 baal41l18 1321 1322 147.5 280 BaH31A03 1323 1324 152.2 280 bastl39A0901 1325 1326 152.2 280 baet46D0507 1327 1328 152.2 283 baal27e20 1329 1330 153.3 283 bags37l16 1331 1332 153.3 283 baak34o06 1333 1334 153.3 286 BaAL30I11 1335 1336 155.4 286 BaH62C15 1337 1338 155.4 286 basd12i15 1339 1340 155.4 286 BaAK40I03 1341 1342 155.4 286 BaAK23E14 1343 1344 155.4 286 BaAL21F11 1345 1346 155.4 286 BaAK16L19 1347 1348 155.4 293 baal27a24 1349 1350 156.5 294 kr41H0315 1351 1352 157.6 294 BaH62I23 1353 1354 157.6 296 bah52i24 1355 1356 158.7 296 bastl33A0301 1357 1358 158.7 296 BaH28J15 1359 1360 158.7 299 bastl56A0301 1361 1362 159.8 299 baal39a03 1363 1364 159.8

TABLE 2-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 242 bah57o03 1265 1266 138.3 242 baak41d22 1267 1268 138.3 242 bah61c16 1269 1270 138.3 242 BaAL7M13 1271 1272 138.3 242 BaAK31F11 1273 1274 138.3 242 basd22k05 1275 1276 138.3 242 basd3p19 1277 1278 138.3 242 baak17o09 1279 1280 138.3 242 baal33d23 1281 1282 138.3 242 BaAK12F04 1283 1284 138.3 242 bah53j16 1285 1286 138.3 262 BaSD2E24 1287 1288 138.5 263 bags39h08 1289 1290 138.7 264 BaSD1N02 1291 1292 139.5 264 bags20m21 1293 1294 139.5 264 baal10h19 1295 1296 139.5 264 basd12m11 1297 1298 139.5 264 bah29d24 1299 1300 139.5 264 BaGS39P09 1301 1302 139.5 264 BaGS31F17 1303 1304 139.5 264 BaGS23D08 1305 1306 139.5 264 bags39d15 1307 1308 139.5 264 BaGS34I17 1309 1310 139.5 274 BaH32N02 1311 1312 141.6 274 BaSD18H19 1313 1314 141.6 274 bast63A0101 1315 1316 141.6 274 BaH50N04 1317 1318 141.6 278 baal4a13 1319 1320 147.5 278 baal41l18 1321 1322 147.5 280 BaH31A03 1323 1324 152.2 280 bastl39A0901 1325 1326 152.2 280 baet46D0507 1327 1328 152.2 283 baal27e20 1329 1330 153.3 283 bagsS7l16 1331 1332 153.3 283 baak34o06 1333 1334 153.3 286 BaAL30I11 1335 1336 155.4 286 BaH62C15 1337 1338 155.4 286 basd12i15 1339 1340 155.4 286 BaAK40I03 1341 1342 155.4 286 BaAK23E14 1343 1344 155.4 286 BaAL21F11 1345 1346 155.4 286 BaAK16L19 1347 1348 155.4 293 baal27a24 1349 1350 156.5 294 kr41H0315 1351 1352 157.6 294 BaH62I23 1353 1354 157.6 296 bah52i24 1355 1356 158.7 296 bastl33A0301 1357 1358 158.7 296 BaH28J15 1359 1360 158.7 299 bastl56A0301 1361 1362 159.8 299 baal39a03 1363 1364 159.8

TABLE 2-8 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 345 kr14F0911 1459 1460 190.8 352 BaGS36A04 1461 1462 192.8 352 bags13a16 1463 1464 192.8 354 bags26e20 1465 1466 196.1 355 baet45G1214 1467 1468 197.6 356 BaAK35F14 1469 1470 202 357 BaAK23N21 1471 1472 203.2 357 basd13j22 1473 1474 203.2 359 BaAK46E10 1475 1476 208.4 359 bastl55F0812 1477 1478 208.4 361 baak46l06 1479 1480 209.4 361 BaAL2D11 1481 1482 209.4 363 baak15p17 1483 1484 210.5 364 BaGS33J16 1485 1486 211.6 364 bags7p21 1487 1488 211.6 366 BaAK22E05 1489 1490 212.7 366 bah42m05 1491 1492 212.7 368 BaAK25L01 1493 1494 213.8 369 bags39e24 1495 1496 214.9 369 BaH56N24 1497 1498 214.9 371 baak44i12 1499 1500 215.4 372 bah21j03 1501 1502 215.9 373 kr71B0103 1503 1504 217.4 373 BaGS16D15 1505 1506 217.4 375 baak21p23 1507 1508 217.9 376 BaGS6G09 1509 1510 219 376 BaSD15M02 1511 1512 219 376 basd13f02 1513 1514 219 376 BaH19F21 1515 1516 219 380 bags20b10 1517 1518 224.5 380 bah26j10 1519 1520 224.5 382 bast65G0113 1521 1522 225.5 383 baak4k13 1523 1524 226.5 383 baal19j23 1525 1526 226.5 383 bags34h11 1527 1528 226.5 386 bags37j03 1529 1530 227.6 387 baal15e13 1531 1532 230.5 387 BaAL5O10 1533 1534 230.5 389 BaGS29J10 1535 1536 234.4 390 bah22o08 1537 1538 235.7 390 bags6k13 1539 1540 235.7 392 bastl43C0705 1541 1542 236.8 393 BaAL4D10 1543 1544 237.4 393 basd12n12 1545 1546 237.4 395 bags23h03 1547 1548 240.3 395 bags6l02 1549 1550 240.3 397 bast63B0703 1551 1552 243.4 397 BaSD13E02 1553 1554 243.4 399 BaSD14P15 1555 1556 248.7 399 bah13a17 1557 1558 248.7

TABLE 2-9 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 401 BaH50L23 1559 1560 250.9 401 bags19g04 1561 1562 250.9 403 bast73E0210 1563 1564 252 403 BaH50O21 1565 1566 252 403 basd21g05 1567 1568 252 406 bah33p11 1569 1570 253.1 406 baal5i19 1571 1572 253.1 406 bah16e04 1573 1574 253.1 406 baak32p24 1575 1576 253.1 406 EBmac415 — — 253.1 411 bags38f12 1577 1578 254.2 411 basd26p18 1579 1580 254.2 413 baak45h16 1581 1582 255.3 414 bah28p12 1583 1584 257.5 415 bah19a10 1585 1586 258.6 416 baak13d11 1587 1588 258.8 417 bah49p10 1589 1590 259.1 417 baal32n15 1591 1592 259.1 419 bast09C0305 1593 1594 260.6 420 basd16l09 1595 1596 263.9 420 BaAK22H04 1597 1598 263.9 422 BaGS15J13 1599 1600 265 422 kr66G0414 1601 1602 265 424 BaSD22C07 1603 1604 266.1 424 kr71C1105 1605 1606 266.1 424 bags34i11 1607 1608 266.1 427 BaH23K17 1609 1610 267.3 428 bast39D0107 1611 1612 270.5 428 BaH44K24 1613 1614 270.5 430 BaGS18N21 1615 1616 273.5 430 baak32k15 1617 1618 273.5 432 bah61a13 1619 1620 275.7 432 bags35n11 1621 1622 275.7 432 BaAK24I03 1623 1624 275.7 435 baal4h20 1625 1626 277.5 435 BaH28N23 1627 1628 277.5 435 bags10e13 1629 1630 277.5 438 baak27i10 1631 1632 278.5 438 bags19d13 1633 1634 278.5 438 baak19d04 1635 1636 278.5 438 BaAL34O19 1637 1638 278.5 438 baal12l02 1639 1640 278.5 438 BaAK1P04 1641 1642 278.5 438 baak35m13 1643 1644 278.5 438 bags35a12 1645 1646 278.5 446 bah41n09 1647 1648 279.6 446 BaGS23I12 1649 1650 279.6 448 BaH16P20 1651 1652 280.4 448 BaAK42L17 1653 1654 280.4 450 baak36d23 1655 1656 284.2

TABLE 2-10 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 450 bast78C1006 1657 1658 284.2 452 BaH45O16 1659 1660 285 453 bah21h09 1661 1662 286.1 454 bah58p22 1663 1664 287.2 455 bags20l19 1665 1666 288.8 456 bah13i10 1667 1668 290.4 457 BaAK36B07 1669 1670 291.5 458 baak26b05 1671 1672 295.5 459 baal7c15 1673 1674 296.6 459 bah63f05 1675 1676 296.6 461 bags15j16 1677 1678 297.9 462 BaGS6N10 1679 1680 299.2 462 bah41e10 1681 1682 299.2 464 BaH54D08 1683 1684 299.7 465 baak18c01 1685 1686 300.2 466 basd21o07 1687 1688 301.3 467 bah41b23 1689 1690 301.9 468 basd18g15 1691 1692 302.4 468 baak43c03 1693 1694 302.4 468 bastl30D0408 1695 1696 302.4 468 bah17p16 1697 1698 302.4 472 baal13f18 1699 1700 303.5 472 bags18i22 1701 1702 303.5 472 bags9b02 1703 1704 303.5 475 bah11e22 1705 1706 323.4 476 bah58h09 1707 1708 332.9 477 basd16e16 1709 1710 334.9 478 BaAK4C12 1711 1712 340 479 bags37a05 1713 1714 343.4 480 bags9p10 1715 1716 351.7 481 baak24m01 1717 1718 352.7 482 basd27d09 1719 1720 353.2 483 baak34a14 1721 1722 353.7 483 baak36a20 1723 1724 353.7 483 BaSD17P09 1725 1726 353.7 486 bags5m04 1727 1728 354.8 487 BaGS5E06 1729 1730 355.9 487 MWG2076 — — 355.9 487 bags15f03 1731 1732 355.9 487 bags18j23 1733 1734 355.9 487 baal13m04 1735 1736 355.9 487 bags9o24 1737 1738 355.9 493 kr49E0610 1739 1740 356.4 494 bah12h16 1741 1742 356.9 494 baak33n16 1743 1744 356.9 494 BaGS22E05 1745 1746 356.9 497 bah26n01 1747 1748 358 497 BaGS39E07 1749 1750 358 497 BaH45O03 1751 1752 358 500 BaH38A09 1753 1754 360.2

As shown in Table 3-1 to Table 3-10, the chromosomal order in barley 3H chromosome (distance from the short arm end of 3H chromosome) has been specified for 444 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1755 through SEQ ID NO: 2642. The chromosomal order in barley 3H chromosome has also been specified for 13 known clones (MWG848, HvLTPPB, HVM9, Bmac67, Bmag136, Bmac209, HVM27, HvBRI1, HVM33, HVM60, Bmag225, Bmag13, and HVM62).

TABLE 3-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 BaAL15P01 1755 1756 0 2 BaAK42D06 1757 1758 9.8 2 bags12k16 1759 1760 9.8 2 BaAL36H19 1761 1762 9.8 2 bags1d06 1763 1764 9.8 6 bast18A0602 1765 1766 10.9 6 basd22i04 1767 1768 10.9 8 BaGS31M01 1769 1770 12 9 BaH63H24 1771 1772 12.4 10 basd15n13 1773 1774 12.8 11 MWG848 — — 13.1 11 BaAK13C16 1775 1776 13.1 13 BaGS32C19 1777 1778 15.3 13 baak36f08 1779 1780 15.3 15 bastl04C0406 1781 1782 16.4 15 bastl42D1107 1783 1784 16.4 17 BaGS31B20 1785 1786 17.5 17 baak39l17 1787 1788 17.5 19 BaAL17J24 1789 1790 20.7 19 bah15p03 1791 1792 20.7 21 baak20g06 1793 1794 21.8 21 baal22c16 1795 1796 21.8 21 baak46o05 1797 1798 21.8 24 basd15h22 1799 1800 22.9 25 BaSD19C07 1801 1802 24 26 bags22i13 1803 1804 25.7 27 baak39a14 1805 1806 26.8 28 bah31e12 1807 1808 27.9 28 BaSD15L22 1809 1810 27.9 30 BaGS20D21 1811 1812 30.1 31 bags39o21 1813 1814 30.7 31 basd21j11 1815 1816 30.7 33 BaH48C10 1817 1818 32.3 34 BaH54J07 1819 1820 33.9 35 BaSD19H23 1821 1822 36.1 35 baak35n06 1823 1824 36.1 37 bags35b22 1825 1826 39.3 37 BaAK30H06 1827 1828 39.3 37 BaAL15M07 1829 1830 39.3 40 BaGS20N02 1831 1832 40.4 40 BaAL19L12 1833 1834 40.4 42 bags25b05 1835 1836 41.5 42 HvLTPPB — — 41.5 42 baal1h04 1837 1838 41.5 45 baet46B0903 1839 1840 42.6 45 BaGS19F16 1841 1842 42.6 45 baak13g18 1843 1844 42.6 48 BaH45N12 1845 1846 47 49 bast74H0216 1847 1848 48.1 50 bah24l06 1849 1850 50.5

TABLE 3-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 51 BaAK45C14 1851 1852 53.4 51 baal5k12 1853 1854 53.4 53 kr63F0111 1855 1856 54.1 53 baak13h18 1857 1858 54.1 53 baak12j16 1859 1860 54.1 56 BaAK28J20 1861 1862 55.2 56 BaH27G14 1863 1864 55.2 56 BaH49B13 1865 1866 55.2 59 BaGS35A09 1867 1868 57.4 60 BaGS38L24 1869 1870 58.6 61 BaAK43H20 1871 1872 66.5 61 bast16A0802 1873 1874 66.5 61 basd18k01 1875 1876 66.5 64 BaH58D17 1877 1878 67.6 65 BaAK30M07 1879 1880 69.2 65 bags6a04 1881 1882 69.2 67 kr15H0915 1883 1884 69.7 67 bags26d01 1885 1886 69.7 69 basd14k04 1887 1888 70.8 69 BaSD24D11 1889 1890 70.8 69 BaH53L10 1891 1892 70.8 72 BaH60D22 1893 1894 73 72 BaSD1G06 1895 1896 73 72 BaAK21L13 1897 1898 73 72 bah57m03 1899 1900 73 76 baal30b10 1901 1902 74.1 77 bah62n16 1903 1904 74.7 78 BaGS22F15 1905 1906 75.2 78 BaH50J14 1907 1908 75.2 80 bah19d23 1909 1910 75.7 81 bags38c06 1911 1912 76.2 81 BaGS25N05 1913 1914 76.2 81 bastl29B0503 1915 1916 76.2 84 baak27d01 1917 1918 77.3 84 baak43n21 1919 1920 77.3 86 BaAL4F05 1921 1922 78.4 86 baal4a06 1923 1924 78.4 88 bast17D1008 1925 1926 80 89 BaGS16B17 1927 1928 81.1 90 BaGS27P18 1929 1930 81.7 90 BaGS4J14 1931 1932 81.7 90 BaAK16B19 1933 1934 81.7 90 baal40p07 1935 1936 81.7 94 bah49c19 1937 1938 84 95 bast58C0406 1939 1940 87.4 95 BaAK35M24 1941 1942 87.4 95 bags19h13 1943 1944 87.4 95 bah57c21 1945 1946 87.4 99 BaH53P15 1947 1948 88.5 99 BaGS20A10 1949 1950 88.5

TABLE 3-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 99 bast02D0808 1951 1952 88.5 99 bags31c04 1953 1954 88.5 99 BaH32J06 1955 1956 88.5 99 bah60o22 1957 1958 88.5 99 BaH50C16 1959 1960 88.5 99 BaH57K23 1961 1962 88.5 99 bags21g23 1963 1964 88.5 99 HVM9 — — 88.5 109 Bmac67 — — 89.6 109 baak43e04 1965 1966 89.6 111 kr17G1113 1967 1968 92.8 111 bags19k19 1969 1970 92.8 111 baak38b13 1971 1972 92.8 111 baak1d12 1973 1974 92.8 111 BaH28M14 1975 1976 92.8 116 BaH48I15 1977 1978 93.9 116 basd27h23 1979 1980 93.9 116 BaAK21A11 1981 1982 93.9 119 bast04B0804 1983 1984 94.4 120 bah45f13 1985 1986 94.9 120 BaAL8J18 1987 1988 94.9 120 BaGS9D01 1989 1990 94.9 120 baal12d12 1991 1992 94.9 120 baal4i06 1993 1994 94.9 125 bah26i01 1995 1996 96.5 125 BaGS15C17 1997 1998 96.5 127 baak1k08 1999 2000 97 127 bags9b03 2001 2002 97 127 baet42G1214 2003 2004 97 127 bah18d12 2005 2006 97 127 BaSD14G02 2007 2008 97 127 bags22b22 2009 2010 97 127 bah13f10 2011 2012 97 134 baal36g05 2013 2014 97.5 135 bags33j15 2015 2016 98 135 BaAL12H04 2017 2018 98 135 BaGS16I18 2019 2020 98 138 bast61D0707 2021 2022 98.5 139 bah11k22 2023 2024 99 139 baak32p21 2025 2026 99 139 bah63l21 2027 2028 99 139 BaGS38D03 2029 2030 99 143 BaSD23A04 2031 2032 100.1 144 BaSD14C15 2033 2034 101.2 144 bast63C0105 2035 2036 101.2 144 bast23C1105 2037 2038 101.2 147 bags23b01 2039 2040 102.3 147 bags29c09 2041 2042 102.3 147 bags6b06 2043 2044 102.3 147 BaAK27G06 2045 2046 102.3

TABLE 3-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 147 BaAK39I07 2047 2048 102.3 147 BaGS20G21 2049 2050 102.3 147 bastl22E0810 2051 2052 102.3 147 bah11m03 2053 2054 102.3 147 BaAL3C04 2055 2056 102.3 156 BaAL19H10 2057 2058 103.4 156 bags24n16 2059 2060 103.4 156 BaSD24B15 2061 2062 103.4 156 bah48n17 2063 2064 103.4 156 bags9i05 2065 2066 103.4 156 bags1l22 2067 2068 103.4 156 bah62p18 2069 2070 103.4 156 bah55n21 2071 2072 103.4 156 bah44a05 2073 2074 103.4 156 bah19c13 2075 2076 103.4 156 baal12b04 2077 2078 103.4 156 Bmag136 — — 103.4 156 Bmac209 — — 103.4 156 BaAK28A10 2079 2080 103.4 156 kr28B0703 2081 2082 103.4 156 bastl50C0606 2083 2084 103.4 156 BaGS36B01 2085 2086 103.4 156 BaH19A05 2087 2088 103.4 156 baal10c06 2089 2090 103.4 156 bags19p04 2091 2092 103.4 176 BaAK21A17 2093 2094 104.5 176 bah20j14 2095 2096 104.5 176 BaAK19A03 2097 2098 104.5 176 BaGS30E19 2099 2100 104.5 176 BaAK28C21 2101 2102 104.5 176 bags11o14 2103 2104 104.5 182 BaGS13P22 2105 2106 105.6 182 kr27A1101 2107 2108 105.6 182 HVM27 — — 105.6 185 baal12i18 2109 2110 106.7 186 BaAL8G07 2111 2112 107.8 187 bast58F0412 2113 2114 108.9 187 bags3f23 2115 2116 108.9 189 baal4m06 2117 2118 109.3 190 baal32p23 2119 2120 109.7 191 basd23m17 2121 2122 110 191 bastl30E0509 2123 2124 110 191 bah44b08 2125 2126 110 191 BaGS14N10 2127 2128 110 191 BaGS32B13 2129 2130 110 191 BaH30B03 2131 2132 110 191 basd1l23 2133 2134 110 191 basd11o06 2135 2136 110 191 bah17f24 2137 2138 110 191 BaAK26L17 2139 2140 110

TABLE 3-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 191 bags23f03 2141 2142 110 191 bags20o06 2143 2144 110 203 bags19n12 2145 2146 111.1 203 baal12a09 2147 2148 111.1 203 bast14F0612 2149 2150 111.1 203 baak13p20 2151 2152 111.1 207 BaGS16K13 2153 2154 111.9 207 baak22c16 2155 2156 111.9 209 baal40m06 2157 2158 115.4 210 BaH29I03 2159 2160 116.8 211 bah16h01 2161 2162 117.6 211 bah57a11 2163 2164 117.6 211 BaH41E23 2165 2166 117.6 211 baal11c11 2167 2168 117.6 215 BaSD14L18 2169 2170 118.1 216 bah14d17 2171 2172 118.7 216 BaGS1N17 2173 2174 118.7 216 bags27h17 2175 2176 118.7 219 bah47p22 2177 2178 119.8 219 BaH50A16 2179 2180 119.8 219 bags7b20 2181 2182 119.8 219 bags22a02 2183 2184 119.8 223 HvBRI1 — — 120.4 224 basd12g02 2185 2186 122.6 225 basd15o18 2187 2188 123.1 226 baal25d19 2189 2190 125.3 226 bast52G0414 2191 2192 125.3 228 baal11c20 2193 2194 126.4 229 bags37k06 2195 2196 127 230 bags6e22 2197 2198 127.5 230 basd15a02 2199 2200 127.5 230 bags7b06 2201 2202 127.5 230 bah15k11 2203 2204 127.5 230 bast46C0406 2205 2206 127.5 230 bastl45E0509 2207 2208 127.5 236 baal35p14 2209 2210 128.6 237 BaH41G07 2211 2212 129.3 237 bast46H1016 2213 2214 129.3 239 bah60d12 2215 2216 131.8 239 baal39a19 2217 2218 131.8 241 baak11n24 2219 2220 132.9 241 bast14E0909 2221 2222 132.9 241 baet13G0713 2223 2224 132.9 244 HVM33 — — 135.8 244 BaSD14L04 2225 2226 135.8 246 baak13c05 2227 2228 136 246 bast141G0513 2229 2230 136 248 BaGS39M09 2231 2232 137.6 249 baal19b12 2233 2234 138.6 250 BaAL39B05 2235 2236 144

TABLE 3-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 250 BaAL1J03 2237 2238 144 250 basd11k09 2239 2240 144 253 BaGS33N15 2241 2242 146.1 253 baet43H0416 2243 2244 146.1 253 bah11m06 2245 2246 146.1 253 baak31a24 2247 2248 146.1 253 bags27h20 2249 2250 146.1 253 basd27c09 2251 2252 146.1 259 BaAL25O01 2253 2254 146.6 260 bags7f08 2255 2256 147.1 261 BaGS13O12 2257 2258 148.2 261 bags38b06 2259 2260 148.2 263 BaAK19J09 2261 2262 149.3 263 bags9e16 2263 2264 149.3 265 bags20n14 2265 2266 150.4 265 bah42o12 2267 2268 150.4 265 baak31k16 2269 2270 150.4 265 bags4p14 2271 2272 150.4 265 bah27a22 2273 2274 150.4 270 bags21n02 2275 2276 151.5 271 BaH62B09 2277 2278 153.7 271 BaGS19J10 2279 2280 153.7 273 bags15k18 2281 2282 154.2 274 BaAK29G03 2283 2284 154.7 274 baak23e11 2285 2286 154.7 274 bags31k04 2287 2288 154.7 274 bast56C0305 2289 2290 154.7 278 basd26c09 2291 2292 159 278 BaSD20B11 2293 2294 159 278 HVM60 — — 159 281 BaH46F11 2295 2296 160.1 281 BaH30F03 2297 2298 160.1 281 BaSD27G02 2299 2300 160.1 284 Bmag225 — — 161.2 284 BaAK33I12 2301 2302 161.2 286 baak32m10 2303 2304 162.3 286 BaAL4L02 2305 2306 162.3 286 kr44F0911 2307 2308 162.3 289 BaGS37A16 2309 2310 163.5 290 bags39p06 2311 2312 163.9 290 BaAL12N06 2313 2314 163.9 292 bags22p05 2315 2316 165.4 293 bags9a03 2317 2318 166.5 293 baet24F1212 2319 2320 166.5 295 baak29d10 2321 2322 167.6 295 bags13i12 2323 2324 167.6 295 BaAL16A23 2325 2326 167.6 298 bastl16B1204 2327 2328 171.6 298 bastl29F0711 2329 2330 171.6 300 BaGS32M17 2331 2332 172.1

TABLE 3-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 301 baal15e10 2333 2334 172.5 301 basd3g08 2335 2336 172.5 303 baal33m18 2337 2338 173.6 303 baak24e23 2339 2340 173.6 303 baak46j13 2341 2342 173.6 306 bah49o05 2343 2344 174.7 306 baal15e05 2345 2346 174.7 306 BaAK1O08 2347 2348 174.7 306 BaGS30P02 2349 2350 174.7 310 BaSD15D05 2351 2352 178.9 311 BaAL7B16 2353 2354 179.2 312 bags38n23 2355 2356 180.3 313 baak36a14 2357 2358 181.6 313 baak34g01 2359 2360 181.6 313 baak12f01 2361 2362 181.6 316 BaGS30I18 2363 2364 183.7 316 bags35n24 2365 2366 183.7 316 bah37j21 2367 2368 183.7 316 bah31e13 2369 2370 183.7 320 bags22g10 2371 2372 190 320 bags22f17 2373 2374 190 322 baal30l05 2375 2376 190.9 322 bags38o10 2377 2378 190.9 324 baak40o04 2379 2380 193.1 325 BaH49L21 2381 2382 193.6 325 bastl30A0701 2383 2384 193.6 327 bags19l03 2385 2386 195.1 328 bah18m13 2387 2388 196.6 329 baak44p03 2389 2390 198.2 329 kr10H0216 2391 2392 198.2 331 bags11i04 2393 2394 199.4 332 bah52o06 2395 2396 200.5 333 bah35c14 2397 2398 200.8 333 BaAL30C02 2399 2400 200.8 335 bah11i21 2401 2402 201.9 336 BaAK36B11 2403 2404 204.1 337 BaAK20K23 2405 2406 206 337 basd14n22 2407 2408 206 339 BaAL25P17 2409 2410 207.6 339 BaH49A01 2411 2412 207.6 341 basd13p12 2413 2414 209.5 341 bags39m17 2415 2416 209.5 343 baet44D0707 2417 2418 212.8 344 BaH16P10 2419 2420 213.9 344 BaAK42J01 2421 2422 213.9 346 kr24E0709 2423 2424 215.8 346 BaAK38O08 2425 2426 215.8 348 BaAL13N01 2427 2428 217.7 349 baak12c12 2429 2430 219.8 350 BaGS31N06 2431 2432 220.9

TABLE 3-8 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 351 baal15f24 2433 2434 221.5 352 BaSD18B21 2435 2436 222 352 BaSD17G23 2437 2438 222 354 baak41k22 2439 2440 223.7 355 baak21o03 2441 2442 225.4 356 BaSD16B18 2443 2444 227 356 bast79C1105 2445 2446 227 358 BaAK4J17 2447 2448 228.1 359 BaAL37H08 2449 2450 228.6 359 BaH36L17 2451 2452 228.6 361 BaH46A12 2453 2454 229.7 361 bags11i15 2455 2456 229.7 361 baak20g24 2457 2458 229.7 364 baak20h23 2459 2460 230.8 365 baak35b18 2461 2462 231.9 365 Bmag13 — — 231.9 365 baak41o03 2463 2464 231.9 365 baet46C0206 2465 2466 231.9 369 bags10i20 2467 2468 233 369 baal1b16 2469 2470 233 369 baal5i05 2471 2472 233 369 BaH27B05 2473 2474 233 369 bah14a11 2475 2476 233 369 basd19k10 2477 2478 233 369 bags30n17 2479 2480 233 369 bags31e24 2481 2482 233 369 BaGS5B16 2483 2484 233 378 BaAL5F06 2485 2486 234.1 378 bags34a11 2487 2488 234.1 378 bags6c16 2489 2490 234.1 378 bah42g19 2491 2492 234.1 378 BaH47O14 2493 2494 234.1 383 BaH12L06 2495 2496 235.2 383 basd11a10 2497 2498 235.2 385 BaH50I12 2499 2500 237.3 385 BaH51A21 2501 2502 237.3 385 baak45p02 2503 2504 237.3 388 BaSD12P12 2505 2506 241.6 389 bags9l16 2507 2508 242.7 389 BaSD26O20 2509 2510 242.7 389 bags5d10 2511 2512 242.7 389 baal0e07 2513 2514 242.7 393 baak14e02 2515 2516 243.8 394 BaH63F14 2517 2518 244.9 394 bah33f19 2519 2520 244.9 394 bags19l10 2521 2522 244.9 397 BaH56J21 2523 2524 246 397 baal40i22 2525 2526 246 397 kr42C0105 2527 2528 246 400 bags16i19 2529 2530 246.5

TABLE 3-9 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 400 BaH54H01 2531 2532 246.5 402 kr69E0810 2533 2534 247.8 403 bags17j14 2535 2536 250 404 bags20e19 2537 2538 253 405 bast74C0206 2539 2540 255.1 405 BaAK13L10 2541 2542 255.1 407 bags20i01 2543 2544 256.2 407 basd19n14 2545 2546 256.2 407 baak42g21 2547 2548 256.2 407 BaAK39A15 2549 2550 256.2 411 basd18o21 2551 2552 257.3 411 BaAK22K17 2553 2554 257.3 413 baal6o24 2555 2556 260.9 414 BaH22C09 2557 2558 262.7 414 HVM62 — — 262.7 416 bags23k14 2559 2560 263.8 416 baal24n12 2561 2562 263.8 418 BaGS21H17 2563 2564 268.1 418 BaSD16I09 2565 2566 268.1 418 BaAL13B22 2567 2568 268.1 421 bah41l03 2569 2570 270.2 422 bastl30F0111 2571 2572 272.5 423 BaH15L04 2573 2574 275.9 423 baak40c12 2575 2576 275.9 425 kr23D0408 2577 2578 277 425 bah57d15 2579 2580 277 427 baak31e03 2581 2582 278.1 428 BaAL13O01 2583 2584 281.8 429 BaSD26P04 2585 2586 286.3 430 bags20f18 2587 2588 287.4 431 BaH42J22 2589 2590 288.5 431 BaAL3K03 2591 2592 288.5 431 kr30B1103 2593 2594 288.5 434 baak14i02 2595 2596 289.6 434 bags28c17 2597 2598 289.6 434 baal4e21 2599 2600 289.6 434 BaGS29D05 2601 2602 289.6 438 bah54d24 2603 2604 290.7 439 basd22g20 2605 2606 291.8 439 BaH62H20 2607 2608 291.8 441 bags38h17 2609 2610 292.9 441 BaAK30B23 2611 2612 292.9 441 BaAK24P09 2613 2614 292.9 441 baak23i12 2615 2616 292.9 441 BaGS22D06 2617 2618 292.9 441 BaAL34P18 2619 2620 292.9 441 BaAL39F24 2621 2622 292.9 441 BaGS4L04 2623 2624 292.9 441 bah12e02 2625 2626 292.9 441 BaAL15F23 2627 2628 292.9

TABLE 3-10 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 441 BaGS25O15 2629 2630 292.9 441 BaGS7G14 2631 2632 292.9 441 basd24i11 2633 2634 292.9 454 baak20l21 2635 2636 294 455 bags33m02 2637 2638 295 455 BaH48G21 2639 2640 295 457 bags27p13 2641 2642 300.4

As shown in Table 4-1 to Table 4-7, the chromosomal order in barley 4H chromosome (distance from the short arm end of 4H chromosome) has been specified for 341 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 2643 through SEQ ID NO: 3324. The chromosomal order in barley 4H chromosome has also been specified for 9 known clones (HVM40, MWG2033, HVM3, MWG058, Bmag353, HVM68, HVM67, sh, and VRN2).

TABLE 4-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 BaGS10N17 2643 2644 0 2 BaAK38K23 2645 2646 1.1 2 bah14a22 2647 2648 1.1 4 BaAL19O06 2649 2650 2.2 4 BaGS32P17 2651 2652 2.2 6 bah11j04 2653 2654 3.3 7 baak35b06 2655 2656 4.1 7 basd21f17 2657 2658 4.1 9 BaAK44O11 2659 2660 7 10 bastl14D0408 2661 2662 8.2 11 BaAK42C15 2663 2664 9 12 baak40n12 2665 2666 10 12 basd2j05 2667 2668 10 12 baak46n05 2669 2670 10 12 baak24g04 2671 2672 10 16 BaAL22H02 2673 2674 11 17 bah56g24 2675 2676 16.4 17 BaAL2I22 2677 2678 16.4 19 bags20c22 2679 2680 17.5 20 BaH50O22 2681 2682 18.6 20 baak32f06 2683 2684 18.6 20 bags35i24 2685 2686 18.6 20 BaSD21D13 2687 2688 18.6 20 bast46D0408 2689 2690 18.6 25 bast55B1204 2691 2692 22.1 26 BaGS39M07 2693 2694 24.5 27 bags39b15 2695 2696 27.6 27 baak37p11 2697 2698 27.6 27 bah29o22 2699 2700 27.6 27 baak12k14 2701 2702 27.6 31 baet31E1010 2703 2704 28.7 32 HVM40 — — 29.8 32 baal39h14 2705 2706 29.8 34 bags14g22 2707 2708 30.9 35 baal18m18 2709 2710 32.1 36 BaSD24E02 2711 2712 33.2 36 BaH50N14 2713 2714 33.2 36 kr70A0202 2715 2716 33.2 39 baak11d04 2717 2718 34.3 40 MWG2033 — — 35.4 40 bags20h01 2719 2720 35.4 42 BaH39P15 2721 2722 35.9 43 BaAK46O20 2723 2724 36.4 43 baal33m06 2725 2726 36.4 43 bastl50E0309 2727 2728 36.4 43 bags20i17 2729 2730 36.4 47 BaGS22A07 2731 2732 37.5 48 bah41b09 2733 2734 37.7 49 kr18G0913 2735 2736 39 49 BaGS19O23 2737 2738 39

TABLE 4-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 51 bags15e12 2739 2740 39.6 52 bags29i09 2741 2742 40.1 52 baak19m23 2743 2744 40.1 54 bags14h12 2745 2746 40.6 55 bah32e15 2747 2748 41.2 55 bah11l02 2749 2750 41.2 55 bags39e16 2751 2752 41.2 55 bags10l12 2753 2754 41.2 59 BaGS31M13 2755 2756 42.3 59 baal32m10 2757 2758 42.3 59 basd12b23 2759 2760 42.3 62 baal39e15 2761 2762 43.5 63 baal16l11 2763 2764 45.8 64 BaGS31B01 2765 2766 46.9 64 BaSD17F09 2767 2768 46.9 64 BaH48L11 2769 2770 46.9 67 BaSD14M08 2771 2772 48 67 bah56c09 2773 2774 48 67 baak17g07 2775 2776 48 67 bags20k09 2777 2778 48 67 BaSD25B08 2779 2780 48 67 bast27E0309 2781 2782 48 73 baet20D0107 2783 2784 52.3 74 BaAL17L08 2785 2786 56.3 75 bah61p18 2787 2788 65.2 75 bah63d12 2789 2790 65.2 75 BaAK26A03 2791 2792 65.2 78 baak26n10 2793 2794 67.4 78 bast22B0204 2795 2796 67.4 80 BaAL3C05 2797 2798 68.5 80 BaGS38H14 2799 2800 68.5 80 BaAL4D19 2801 2802 68.5 83 BaAK20B09 2803 2804 69.6 83 BaAK37H01 2805 2806 69.6 83 BaGS33P13 2807 2808 69.6 86 basd13l12 2809 2810 70.7 86 bags11m01 2811 2812 70.7 88 BaGS18H09 2813 2814 77.5 89 basd3d13 2815 2816 86.5 89 baal9m23 2817 2818 86.5 89 bags23f08 2819 2820 86.5 92 BaGS15L23 2821 2822 87.6 92 baak2k13 2823 2824 87.6 92 baak17d18 2825 2826 87.6 92 bags13a12 2827 2828 87.6 96 baak44g22 2829 2830 88.7 97 kr34F0212 2831 2832 89.8 98 baal16d11 2833 2834 92 99 bah22d04 2835 2836 93.1 99 bah48m23 2837 2838 93.1

TABLE 4-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 99 basd15p13 2839 2840 93.1 99 BaGS6O07 2841 2842 93.1 99 BaH37O10 2843 2844 93.1 99 bags21k10 2845 2846 93.1 99 bah56n18 2847 2848 93.1 99 baal9i11 2849 2850 93.1 99 BaAL39N06 2851 2852 93.1 99 bags9d05 2853 2854 93.1 99 BaAL19I19 2855 2856 93.1 99 basd12I11 2857 2858 93.1 99 bastl46D1008 2859 2860 93.1 99 BaH50H16 2861 2862 93.1 99 bags9n05 2863 2864 93.1 99 kr61B1103 2865 2866 93.1 115 kr27B0103 2867 2868 95.2 115 kr33H1115 2869 2870 95.2 115 baak30l02 2871 2872 95.2 115 bah55a12 2873 2874 95.2 115 BaH26K14 2875 2876 95.2 120 HVM3 — — 96.3 120 BaAK33K19 2877 2878 96.3 120 basd18m17 2879 2880 96.3 120 BaH50G09 2881 2882 96.3 120 BaH53B03 2883 2884 96.3 120 BaH54L11 2885 2886 96.3 120 basd13i14 2887 2888 96.3 120 bah56g09 2889 2890 96.3 120 bah41b06 2891 2892 96.3 120 bags14d19 2893 2894 96.3 120 baal20f05 2895 2896 96.3 120 BaH18H12 2897 2898 96.3 120 baal9o21 2899 2900 96.3 120 bags21a02 2901 2902 96.3 120 BaAL3G19 2903 2904 96.3 120 baal7a09 2905 2906 96.3 120 BaAL29I16 2907 2906 96.3 137 bast70B0804 2909 2910 98.4 137 bags14n08 2911 2912 98.4 139 bastl14F0412 2913 2914 99.5 139 BaAK36I12 2915 2916 99.5 139 baal3f19 2917 2916 99.5 142 baal1e10 2919 2920 101.6 142 bags6k02 2921 2922 101.6 142 kr42H0315 2923 2924 101.6 145 bah39p02 2925 2926 102.7 146 BaGS9L14 2927 2928 106 146 baal35l03 2929 2930 106 146 basd12m15 2931 2932 106 146 BaAK35P01 2933 2934 106 146 basd11d18 2935 2936 106

TABLE 4-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 146 BaH38E07 2937 2938 106 146 BaGS18H13 2939 2940 106 146 bags18g16 2941 2942 106 146 bags27f21 2943 2944 106 146 BaGS7M13 2945 2946 106 146 baal39c19 2947 2948 106 146 baak46j05 2949 2950 106 146 BaAK32D20 2951 2952 106 146 MWG058 — — 106 146 BaGS18M02 2953 2954 106 146 bags9c05 2955 2956 106 146 baak46m13 2957 2958 106 146 bags35n16 2959 2960 106 146 bags39g08 2961 2962 106 146 baal33a06 2963 2964 106 146 BaSD17F20 2965 2966 106 146 bags22i21 2967 2968 106 168 bah32g18 2969 2970 107.1 169 BaAL37O23 2971 2972 108.2 169 bags22p03 2973 2974 108.2 171 bags38l18 2975 2976 109.3 172 BaGS35C13 2977 2978 112.6 172 bah47h04 2979 2980 112.6 174 bags30m11 2981 2982 114.7 174 BaH22L15 2983 2984 114.7 176 bags39e15 2985 2986 115.8 177 kr65H0816 2987 2988 116.9 177 basd11h11 2989 2990 116.9 177 baak2b06 2991 2992 116.9 177 Bmag353 — — 116.9 177 bags37j11 2993 2994 116.9 177 BaAK46L15 2995 2996 116.9 183 BaSD14A23 2997 2998 122.3 183 bast25C0705 2999 3000 122.3 185 bast21B1204 3001 3002 123.3 186 bags1l16 3003 3004 129.7 186 BaAK21G02 3005 3006 129.7 188 bastl26E1109 3007 3008 130.6 188 basd13k24 3009 3010 130.6 188 BaAL13F02 3011 3012 130.6 191 bags13c10 3013 3014 134.7 191 bags11o11 3015 3016 134.7 191 BaAK36P01 3017 3018 134.7 194 bah52d09 3019 3020 135.9 194 BaGS26D18 3021 3022 135.9 196 baak33c22 3023 3024 137 196 basd1d10 3025 3026 137 196 baak11c22 3027 3028 137 196 bags34l06 3029 3030 137 196 baak33j06 3031 3032 137

TABLE 4-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 196 bags23a11 3033 3034 137 196 bags34f02 3035 3036 137 196 bags20p21 3037 3038 137 196 bah52m01 3039 3040 137 196 basd15d07 3041 3042 137 196 BaAL20A14 3043 3044 137 207 BaGS21B04 3045 3046 137.7 208 HVM68 — — 138.1 209 BaAL36B07 3047 3048 139.2 209 basd11p10 3049 3050 139.2 209 BaGS33E17 3051 3052 139.2 212 kr67C0206 3053 3054 140.3 213 bags15i20 3055 3056 141.4 214 BaH15E05 3057 3058 142.5 215 BaH58K02 3059 3060 143.6 216 basd13d17 3061 3062 146.9 216 BaGS17A15 3063 3064 146.9 216 BaAK30F13 3065 3066 146.9 216 baal17m22 3067 3068 146.9 216 baak42f04 3069 3070 146.9 216 baal2n22 3071 3072 146.9 222 kr30C0705 3073 3074 148 222 BaSD19J21 3075 3076 148 222 BaH34N22 3077 3078 148 225 BaAK2I20 3079 3080 149.1 225 BaGS1E22 3081 3082 149.1 225 baak34b17 3083 3084 149.1 228 BaSD11L18 3085 3086 151.3 228 kr32A0202 3087 3088 151.3 230 bags27h21 3089 3090 151.9 231 bah62d17 3091 3092 152.4 231 bah43e22 3093 3094 152.4 231 BaAL30I23 3095 3096 152.4 231 BaSD13H20 3097 3098 152.4 231 BaSD14O04 3099 3100 152.4 236 baak34p06 3101 3102 153.5 237 bah63b08 3103 3104 156.8 238 bastl03F0812 3105 3106 157.9 239 basd14m17 3107 3108 159.2 240 BaSD2J03 3109 3110 162.2 241 bah13b17 3111 3112 171.1 242 baal32b23 3113 3114 176.5 242 bastl50D1208 3115 3116 176.5 242 BaGS9H13 3117 3118 176.5 242 baal33e04 3119 3120 176.5 246 BaAL40L16 3121 3122 177.6 247 bah44n03 3123 3124 178.7 247 bags20h05 3125 3126 178.7 247 bags20fM5 3127 3128 178.7 250 BaGS7E03 3129 3130 179.8

TABLE 4-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 250 bags9k13 3131 3132 179.8 252 bast55E0709 3133 3134 181.1 253 bags8a14 3135 3136 184.6 254 baak38k04 3137 3138 185.1 255 BaH33B15 3139 3140 185.6 256 bags29m17 3141 3142 186.7 257 BaSD23P08 3143 3144 187.8 257 BaH42L12 3145 3146 187.8 259 bast79G0313 3147 3148 191 259 baet46C0905 3149 3150 191 259 baal4o09 3151 3152 191 259 bah26e10 3153 3154 191 263 BaH32J04 3155 3156 193.1 264 bags20l07 3157 3158 194.2 265 kr18C0505 3159 3160 195.3 266 basd11m24 3161 3162 199.7 266 basd1i15 3163 3164 199.7 266 bah45b02 3165 3166 199.7 269 bast40C0206 3167 3168 201.8 269 baal40g05 3169 3170 201.8 269 bah13i21 3171 3172 201.8 269 basd27o20 3173 3174 201.8 269 BaAK12L24 3175 3176 201.8 274 BaAL12F24 3177 3178 204 274 baak45l08 3179 3180 204 274 BaAL36N04 3181 3182 204 274 baak41d17 3183 3184 204 274 kr39E0810 3185 3186 204 274 BaGS32G16 3187 3188 204 274 BaGS25M06 3189 3190 204 274 BaH36F21 3191 3192 204 274 BaAK13B12 3193 3194 204 283 baal29j18 3195 3196 205.1 284 bast63B0604 3197 3198 206.2 284 baak11n06 3199 3200 206.2 286 kr13F1012 3201 3202 208.4 287 bags20k06 3203 3204 209.5 288 baak15p20 3205 3206 210.6 288 bah18n11 3207 3208 210.6 290 BaH23J08 3209 3210 213.9 291 baet30B1004 3211 3212 215 291 bags34p06 3213 3214 215 291 bastl33H0816 3215 3216 215 294 BaSD17I17 3217 3218 216.1 294 basd19p22 3219 3220 216.1 294 bags4e03 3221 3222 216.1 294 BaAK36A13 3223 3224 216.1 294 bags33i03 3225 3226 216.1 299 BaAK14F03 3227 3228 217.2 300 BaAK42K19 3229 3230 219.3

TABLE 4-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 300 bast60A1101 3231 3232 219.3 302 bah52l06 3233 3234 220.4 302 bah23h10 3235 3236 220.4 302 BaGS15O23 3237 3238 220.4 302 BaAK20E08 3239 3240 220.4 306 kr61A1202 3241 3242 222.6 307 bast03F0212 3243 3244 223.7 307 bags39m22 3245 3246 223.7 309 BaAK30O17 3247 3248 227.3 310 baak29a01 3249 3250 232.1 310 basd1e04 3251 3252 232.1 312 BaGS23K09 3253 3254 233 313 BaAL2N04 3255 3256 234.3 314 BaH42C12 3257 3258 235.5 315 bah39o14 3259 3260 236.1 316 bast26A1202 3261 3262 236.6 317 BaGS30N15 3263 3264 237.4 317 BaAL34D18 3265 3266 237.4 319 baak15k23 3267 3268 240.3 319 bags37i06 3269 3270 240.3 319 BaGS19N09 3271 3272 240.3 322 BaSD13H09 3273 3274 241.4 323 bags3h19 3275 3276 242.5 323 baak28o08 3277 3278 242.5 325 BaH15K08 3279 3280 243.6 325 HVM67 — — 243.6 325 bah17h20 3281 3282 243.6 325 BaAL5L13 3283 3284 243.6 325 BaGS31P13 3285 3286 243.6 330 BaH28A11 3287 3288 247.9 330 BaSD25E01 3289 3290 247.9 330 sh — — 247.9 333 baet23F0111 3291 3292 249 333 VRN2 — — 249 333 bastl41C0806 3293 3294 249 333 basd11c04 3295 3296 249 333 bags22k17 3297 3298 249 338 bastl27A1101 3299 3300 250.2 338 bags22a16 3301 3302 250.2 340 BaAL5E04 3303 3304 250.3 341 baal39f20 3305 3306 250.5 342 baak43d13 3307 3308 250.7 342 baak41l23 3309 3310 250.7 344 baet23B0604 3311 3312 252.3 345 BaAK28I08 3313 3314 255.6 346 bastl26C1206 3315 3316 258.8 346 bah27p20 3317 3318 258.8 346 baal12d24 3319 3320 258.8 346 bastl41F0511 3321 3322 258.8 346 BaGS17E03 3323 3324 258.8

As shown in Table 5-1 to Table 5-11, the chromosomal order in barley 5H chromosome (distance from the short arm end of 5H chromosome) has been specified for 498 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 3325 through SEQ ID NO: 4320. The chromosomal order in barley 5H chromosome has also been specified for 8 known clones (MWG502, Bmac113, HVM30, Bmag223, HvLOX, MWG2077, MWG2249, and HVM6).

TABLE 5-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 baal10j04 3325 3326 0 1 BaAK33J17 3327 3328 0 1 BaAL21J19 3329 3330 0 4 bast17H0515 3331 3332 0.5 5 bags22c02 3333 3334 1 6 BaAK35A06 3335 3336 2.2 7 BaGS11K08 3337 3338 5.6 8 bastl30A0602 3339 3340 6.7 9 BaGS39H16 3341 3342 8.8 9 MWG502 — — 8.8 11 BaAK18M22 3343 3344 9.9 12 bast26G0614 3345 3346 11.5 13 BaSD22F13 3347 3348 15.9 14 BaAK28L16 3349 3350 20.5 15 bags35o06 3351 3352 24.9 16 bags13d07 3353 3354 25.9 16 BaAL24F18 3355 3356 25.9 16 bah63l18 3357 3358 25.9 19 BaGS9H22 3359 3360 26.4 20 BaAK18A05 3361 3362 26.9 20 BaH38D03 3363 3364 26.9 20 BaH17N17 3365 3366 26.9 23 bah18d08 3367 3368 30.1 24 bags34a05 3369 3370 31.9 24 bags1m23 3371 3372 31.9 24 BaH50B05 3373 3374 31.9 24 bags1h11 3375 3376 31.9 28 BaH47G19 3377 3378 32.4 29 BaAK38H10 3379 3380 32.9 29 bah47e01 3381 3382 32.9 29 bast52E0109 3383 3384 32.9 32 bags1o08 3385 3386 34 32 BaH31H16 3387 3388 34 34 baak32l14 3389 3390 36.1 34 BaH50O06 3391 3392 36.1 34 BaSD26I01 3393 3394 36.1 37 bags1h24 3395 3396 38.3 38 basd27g16 3397 3398 40.5 38 BaGS16G24 3399 3400 40.5 40 BaGS34C19 3401 3402 43.7 40 bah20k17 3403 3404 43.7 40 bah54e13 3405 3406 43.7 40 bags35i06 3407 3408 43.7 40 bah29g09 3409 3410 43.7 45 BaAK29C12 3411 3412 44.8 46 bah56c06 3413 3414 48.2 47 baal6a09 3415 3416 51.8 48 bags6j06 3417 3418 53.1 49 BaH30P15 3419 3420 54.2 50 baak30k04 3421 3422 55.3

TABLE 5-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 51 basd25d22 3423 3424 56 51 BaGS39M02 3425 3426 56 53 baak13n10 3427 3428 58.9 53 BaGS18J21 3429 3430 58.9 53 BaSD22G22 3431 3432 58.9 53 bags18b17 3433 3434 58.9 53 bags33j02 3435 3436 58.9 53 baak36b16 3437 3438 58.9 59 bags37f05 3439 3440 60 59 BaAL17L15 3441 3442 60 61 BaGS20H12 3443 3444 62.4 62 kr65A0802 3445 3446 64 62 BaGS13E12 3447 3448 64 64 basd3h06 3449 3450 65.2 65 basd22j16 3451 3452 66.3 65 bah39n18 3453 3454 66.3 67 bags20d19 3455 3456 67.4 67 BaGS34H19 3457 3458 67.4 69 baak42n10 3459 3460 68.5 69 BaSD24F03 3461 3462 68.5 69 BaGS23N21 3463 3464 68.5 69 BaAK45E04 3465 3466 68.5 69 bags5n23 3467 3468 68.5 74 bast52H1216 3469 3470 69.4 75 bags22f23 3471 3472 69.7 76 BaH38E02 3473 3474 70.5 76 BaH50O07 3475 3476 70.5 78 kr07C1006 3477 3478 73.3 78 baak26a02 3479 3480 73.3 78 BaAK2J22 3481 3482 73.3 78 bah45h23 3483 3484 73.3 82 bah11h09 3485 3486 74.8 82 BaAK46M16 3487 3488 74.8 84 bah55m23 3489 3490 75.2 84 bah59c05 3491 3492 75.2 84 basd11l17 3493 3494 75.2 84 bah19e08 3495 3496 75.2 84 BaAK21N24 3497 3498 75.2 84 BaH50E09 3499 3500 75.2 84 BaAK17P18 3501 3502 75.2 84 BaAL7A04 3503 3504 75.2 84 bast47B0303 3505 3506 75.2 84 bah28o17 3507 3508 75.2 84 bah37k03 3509 3510 75.2 84 bastl38A0501 3511 3512 75.2 84 bags23c03 3513 3514 75.2 84 basd13j01 3515 3516 75.2 84 bags21c02 3517 3518 75.2 84 bah28l03 3519 3520 75.2 84 BaAK36B17 3521 3522 75.2

TABLE 5-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 84 bags6k10 3523 3524 75.2 84 BaAK32J23 3525 3526 75.2 84 bags9k18 3527 3528 75.2 84 baal18c19 3529 3530 75.2 84 baak27p14 3531 3532 75.2 84 BaGS30N21 3533 3534 75.2 84 bah55p23 3535 3536 75.2 84 bah54p22 3537 3538 75.2 84 bags34f05 3539 3540 75.2 84 Bmac113 — — 75.2 84 bags15i11 3541 3542 75.2 84 BaH26I21 3543 3544 75.2 84 BaSD20M22 3545 3546 75.2 114 bah14a24 3547 3548 75.6 115 BaH50J11 3549 3550 76 116 bags7a20 3551 3552 76.4 116 bah25l12 3553 3554 76.4 116 bags6k09 3555 3556 76.4 116 BaGS29P21 3557 3558 76.4 116 bah58f18 3559 3560 76.4 116 bags39i18 3561 3562 76.4 122 basd22l21 3563 3564 77.1 123 bags4o22 3565 3566 77.5 124 kr27E0909 3567 3568 78 125 bastl41H0216 3569 3570 78.5 125 baak36o17 3571 3572 78.5 125 BaH31P15 3573 3574 78.5 125 kr07D0208 3575 3576 78.5 125 bags9h03 3577 3578 78.5 130 BaGS38M11 3579 3580 79.6 130 kr26C0705 3581 3582 79.6 130 bags3k24 3583 3584 79.6 130 bah53e16 3585 3586 79.6 130 bags21n10 3587 3588 79.6 130 BaGS25E06 3589 3590 79.6 130 bah38n03 3591 3592 79.6 130 BaAK38G14 3593 3594 79.6 130 baal5a06 3595 3596 79.6 130 basd26d19 3597 3598 79.6 140 BaSD26D07 3599 3600 80.7 140 BaH50G14 3601 3602 80.7 140 bah21h17 3603 3604 80.7 140 bags5d21 3605 3606 80.7 140 BaAK34D14 3607 3608 80.7 140 baak18a16 3609 3610 80.7 140 bags4b01 3611 3612 80.7 140 bags38c19 3613 3614 80.7 140 BaGS38J23 3615 3616 80.7 140 kr42D0208 3617 3618 80.7 140 BaH33A16 3619 3620 80.7

TABLE 5-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 140 bags10e03 3621 3622 80.7 140 bags14f17 3623 3624 80.7 140 BaAK35N11 3625 3626 80.7 140 bags26n10 3627 3628 80.7 140 bags37n02 3629 3630 80.7 140 baal39l05 3631 3632 80.7 157 BaAK26L11 3633 3634 81.3 158 BaH57C19 3635 3636 81.7 158 bags7a01 3637 3638 81.7 158 bah19l15 3639 3640 81.7 158 baal4o02 3641 3642 81.7 158 bah58a12 3643 3644 81.7 158 HVM30 — — 81.7 158 bah11b08 3645 3646 81.7 158 bags14m15 3647 3648 81.7 158 baak1e17 3649 3650 81.7 167 bags14i11 3651 3652 83.2 168 bastl04B1103 3653 3654 87 169 bags13g08 3655 3656 94.7 170 basd27p03 3657 3658 102.2 170 bast38D0707 3659 3660 102.2 172 baak35j18 3661 3662 103.7 172 baal19k05 3663 3664 103.7 174 BaH19C21 3665 3666 104.2 174 bah49g10 3667 3668 104.2 174 bags14h08 3669 3670 104.2 174 bags23g18 3671 3672 104.2 174 bags22o22 3673 3674 104.2 174 BaAL20A03 3675 3676 104.2 174 bags5e24 3677 3678 104.2 174 bags5f11 3679 3680 104.2 174 BaAK33B10 3681 3682 104.2 174 BaSD15J20 3683 3684 104.2 174 bags38b22 3685 3686 104.2 185 bah39b06 3687 3688 105.3 185 bags19j08 3689 3690 105.3 187 BaGS1G09 3691 3692 106.4 188 bah63j06 3693 3694 107.5 188 BaAL39J02 3695 3696 107.5 188 BaSD27H14 3697 3698 107.5 191 BaH50M01 3699 3700 112 192 BaSD24E13 3701 3702 113.3 193 baal5j24 3703 3704 116.7 194 bah15h18 3705 3706 117.8 194 bast62E0610 3707 3708 117.8 196 bah28k24 3709 3710 118.9 196 bah47c11 3711 3712 118.9 196 BaSD26M15 3713 3714 118.9 196 bah60o02 3715 3716 118.9 196 BaH50G06 3717 3718 118.9

TABLE 5-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 201 bast15G0913 3719 3720 120 201 bah62n12 3721 3722 120 201 baal10n23 3723 3724 120 204 bags20c12 3725 3726 121.1 205 basd16f13 3727 3728 122.2 206 baak21h06 3729 3730 124.2 207 bags3b05 3731 3732 125.2 208 bags22p08 3733 3734 128.3 208 BaH23N06 3735 3736 128.3 208 bags29c08 3737 3738 128.3 211 BaGS11O23 3739 3740 129.4 211 baak16a11 3741 3742 129.4 213 baak15n22 3743 3744 131.6 213 BaAL18J13 3745 3746 131.6 213 bah15m02 3747 3748 131.6 213 baak38d20 3749 3750 131.6 213 BaSD12L21 3751 3752 131.6 213 kr68B1103 3753 3754 131.6 219 BaAK28L22 3755 3756 133.7 219 bags15b10 3757 3758 133.7 219 bags20h21 3759 3760 133.7 219 BaAK29K06 3761 3762 133.7 219 bags21l05 3763 3764 133.7 219 bah56j14 3765 3766 133.7 219 basd15e02 3767 3768 133.7 226 bags3j24 3769 3770 134.8 226 bags38m08 3771 3772 134.8 228 bah11m18 3773 3774 136.9 228 bags35g06 3775 3776 136.9 228 bags37b01 3777 3778 136.9 228 BaAL19F02 3779 3780 136.9 232 BaAL4D09 3781 3782 138 232 BaAK30H10 3783 3784 138 234 bags38b16 3785 3786 142.3 235 bags27f15 3787 3788 143.7 236 bags10k14 3789 3790 146.6 236 bags22f10 3791 3792 146.6 236 baak38n21 3793 3794 146.6 236 baak30e05 3795 3796 146.6 236 bastl04A0101 3797 3798 146.6 236 BaAK28P18 3799 3800 146.6 236 BaGS34E01 3801 3802 146.6 236 BaH54H04 3803 3804 146.6 236 bah13o19 3805 3806 146.6 245 BaAK29B22 3807 3808 147.7 245 BaAK27C16 3809 3810 147.7 245 BaGS22H13 3811 3812 147.7 245 BaSD27A15 3813 3814 147.7 249 BaAK27E07 3815 3816 151 250 BaGS9N02 3817 3818 152.1

TABLE 5-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 251 Bmag223 — — 153.2 252 bags28o18 3819 3820 154.3 253 baal25b05 3821 3822 155.4 253 bastl30A0202 3823 3824 155.4 253 bags37d20 3825 3826 155.4 256 BaGS22K12 3827 3828 155.8 257 BaAK13G12 3829 3830 156.1 258 basd2a02 3831 3832 156.5 258 BaGS31L06 3833 3834 156.5 258 bags17j17 3835 3836 156.5 258 baal12j15 3837 3838 156.5 258 BaAK19H14 3839 3840 156.5 263 BaH34J11 3841 3842 159.8 263 BaGS30I11 3843 3844 159.8 265 BaH49L06 3845 3846 161 265 baal18m24 3847 3848 161 267 baak15l06 3849 3850 163.2 267 basd18o14 3851 3852 163.2 269 bags24p22 3853 3854 164.3 269 BaAL37A09 3855 3856 164.3 269 bags5l01 3857 3858 164.3 269 BaAK12P03 3859 3860 164.3 273 baak21f10 3861 3862 164.5 274 bastl30E0410 3863 3864 164.7 274 kr25C0206 3865 3866 164.7 276 baak4j01 3867 3868 166.2 276 BaAK27E01 3869 3870 166.2 278 bah54n06 3871 3872 167.3 278 bags19p05 3873 3874 167.3 278 bast22F0311 3875 3876 167.3 281 basd1m04 3877 3878 170.6 282 bags35i04 3879 3880 171.7 282 baak41d01 3881 3882 171.7 282 baal19m08 3883 3884 171.7 282 baak13j10 3885 3886 171.7 282 baak44h11 3887 3888 171.7 287 baal19j09 3889 3890 172.2 288 baet37C1105 3891 3892 172.7 288 baak12d06 3893 3894 172.7 288 basd23f10 3895 3896 172.7 288 BaH41P07 3897 3898 172.7 292 baak1g13 3899 3900 173.8 293 basd14b04 3901 3902 174.9 294 bags22l14 3903 3904 177.1 294 bast26E1210 3905 3906 177.1 296 baet25F0911 3907 3908 180 296 BaAK32N13 3909 3910 180 298 BaSD2C09 3911 3912 180.3 299 BaGS31P01 3913 3914 180.7 299 baak30m11 3915 3916 180.7

TABLE 5-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 301 BaAK4N16 3917 3918 181.2 302 bastl46F1012 3919 3920 181.7 302 bags3e06 3921 3922 181.7 302 bags31o10 3923 3924 181.7 305 bags33j12 3925 3926 182.8 306 BaGS30N07 3927 3928 183.8 307 BaH37I18 3929 3930 188.1 307 baak46p24 3931 3932 188.1 309 baak46o19 3933 3934 188.7 310 basd3c19 3935 3936 189.2 310 bags30f01 3937 3938 189.2 310 BaGS20F10 3939 3940 189.2 313 BaGS32D08 3941 3942 191.4 314 BaAK23M23 3943 3944 193.6 314 bah21a16 3945 3946 193.6 314 BaAL3M08 3947 3948 193.6 314 BaAK21I09 3949 3950 193.6 314 baet33A0301 3951 3952 193.6 319 baak24k02 3953 3954 195.7 319 bah33p03 3955 3956 195.7 319 bast75D1208 3957 3958 195.7 322 BaSD12K20 3959 3960 196 323 bags34f06 3961 3962 196.4 324 bags6f09 3963 3964 196.8 324 bags7b10 3965 3966 196.8 326 BaGS25H01 3967 3968 199 326 baak33m08 3969 3970 199 326 bags37e01 3971 3972 199 326 bags32n20 3973 3974 199 326 BaH47A11 3975 3976 199 326 BaGS24M06 3977 3978 199 332 bags22m23 3979 3980 200.1 332 basd18d18 3981 3982 200.1 332 bastl06F0212 3983 3984 200.1 332 BaH49O16 3985 3986 200.1 332 bags39e22 3987 3988 200.1 332 BaH38N06 3989 3990 200.1 332 BaH56P16 3991 3992 200.1 339 BaSD13O13 3993 3994 200.3 340 bags19i06 3995 3996 201.4 340 bah34f11 3997 3998 201.4 340 bags37g04 3999 4000 201.4 343 basd11k21 4001 4002 201.7 344 HvLOX — — 202.8 345 baak43o03 4003 4004 203.6 345 BaAL4J21 4005 4006 203.6 345 BaH51J22 4007 4008 203.6 345 bah58l03 4009 4010 203.6 345 BaGS21M18 4011 4012 203.6 345 BaGS31K06 4013 4014 203.6

TABLE 5-8 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 351 baal4o01*VRN2(sh2) 4015 4016 204 352 BaAK24H19 4017 4018 204.6 352 BaGS17D21 4019 4020 204.6 352 bah39o04 4021 4022 204.6 352 baak46e06 4023 4024 204.6 352 basd13j03 4025 4026 204.6 357 bast14H0416 4027 4028 206.8 357 BaSD18I03 4029 4030 206.8 357 bastl44H1115 4031 4032 206.8 357 bast34D0608 4033 4034 206.8 357 BaGS30F06 4035 4036 206.8 357 bags21k16 4037 4038 206.8 363 BaH48G01 4039 4040 207.9 363 bags39a24 4041 4042 207.9 363 bags15i03 4043 4044 207.9 363 bags23f02 4045 4046 207.9 363 bastl40E1010 4047 4048 207.9 368 bags17p10 4049 4050 211 369 BaH57L13 4051 4052 212.6 369 bags10e22 4053 4054 212.6 371 bags4p07 4055 4056 213.6 371 bah63a08 4057 4058 213.6 373 baak12f13 4059 4060 214.7 373 bah56j15 4061 4062 214.7 373 bah15e16 4063 4064 214.7 373 BaH50F21 4065 4066 214.7 373 basd27o16 4067 4068 214.7 378 kr66G0713 4069 4070 216.2 378 baak46c17 4071 4072 216.2 380 baet19C1206 4073 4074 216.7 380 bags34e15 4075 4076 216.7 380 baal4d18 4077 4078 216.7 380 bah13b13 4079 4080 216.7 384 BaGS26G20 4081 4082 217.2 385 BaGS28C14 4083 4084 217.7 385 kr61G1214 4085 4086 217.7 385 bags9g08 4087 4088 217.7 385 bags5p01 4089 4090 217.7 385 BaAL4D04 4091 4092 217.7 385 bah26p09 4093 4094 217.7 385 bah11e02 4095 4096 217.7 385 MWG2077 — — 217.7 385 bast41F0311 4097 4098 217.7 385 bastl54D0307 4099 4100 217.7 395 BaSD3F21 4101 4102 219.9 395 bah56f18 4103 4104 219.9 397 BaH41C21 4105 4106 221 397 basd19g21 4107 4108 221 399 kr33H0816 4109 4110 223.2 399 BaH38F16 4111 4112 223.2

TABLE 5-9 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 399 BaGS21P09 4113 4114 223.2 402 BaGS36M20 4115 4116 224.2 403 bags21m10 4117 4118 228.5 403 BaAK14G11 4119 4120 228.5 405 BaGS30L20 4121 4122 230.7 406 basd21g02 4123 4124 231.8 406 bah22m17 4125 4126 231.8 406 BaGS9H19 4127 4128 231.8 409 bah26m24 4129 4130 232.9 410 BaH56D01 4131 4132 234 411 bah53i08 4133 4134 237.1 411 bags21d10 4135 4136 237.1 411 bags9a01 4137 4138 237.1 414 baal9m13 4139 4140 238.3 414 baak20m05 4141 4142 238.3 416 baak21k16 4143 4144 238.6 417 bags34d17 4145 4146 239.4 418 baal40k24 4147 4148 240.5 419 BaGS19O14 4149 4150 243.4 419 baak35m03 4151 4152 243.4 419 baak33o23 4153 4154 243.4 419 kr57F1012 4155 4156 243.4 419 bah53i05 4157 4158 243.4 419 bags22l16 4159 4160 243.4 419 baet43C0505 4161 4162 243.4 419 BaH33H02 4163 4164 243.4 419 bastl22E1010 4165 4166 243.4 428 BaH53N24 4167 4168 244.6 429 baal8e24 4169 4170 245.9 429 BaH62H21 4171 4172 245.9 431 BaH42K01 4173 4174 250.7 432 basd26p09 4175 4176 252.1 433 bags4p18 4177 4178 253.1 433 BaH60H14 4179 4180 253.1 433 bast70D1107 4181 4182 253.1 433 bah26i23 4183 4184 253.1 437 baal39g02 4185 4186 253.6 437 bags10e02 4187 4188 253.6 439 bags22c13 4189 4190 255.1 439 bags35n03 4191 4192 255.1 439 BaAL16H03 4193 4194 255.1 442 BaAL15N07 4195 4196 255.8 443 bags18o20 4197 4198 258 444 bags37g12 4199 4200 258.4 445 bags22l12 4201 4202 259.5 446 BaAK44K01 4203 4204 260.6 446 BaAK31G07 4205 4206 260.6 446 bah53n21 4207 4208 260.6 449 BaGS19C02 4209 4210 263.8 450 BaH52E20 4211 4212 265.9

TABLE 5-10 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 450 BaAL4N05 4213 4214 265.9 450 bags7p06 4215 4216 265.9 453 bast70C0905 4217 4218 270.9 453 BaSD15J01 4219 4220 270.9 455 BaH56H18 4221 4222 272.9 455 BaGS4N04 4223 4224 272.9 455 bags31o13 4225 4226 272.9 455 kr29D0107 4227 4228 272.9 459 BaAK21A15 4229 4230 274 459 bags7h14 4231 4232 274 459 basd26b20 4233 4234 274 459 baal1g06 4235 4236 274 459 bags5n14 4237 4238 274 464 BaAK34H17 4239 4240 274.5 465 bastl32H0816 4241 4242 275 465 basd18m12 4243 4244 275 465 baak31p06 4245 4246 275 465 bah52i03 4247 4248 275 469 BaAK33K05 4249 4250 276.1 469 BaAK44J08 4251 4252 276.1 469 baak18g16 4253 4254 276.1 469 baak11a11 4255 4256 276.1 473 BaH25B20 4257 4258 279.3 473 bast12G0113 4259 4260 279.3 473 baal9n02 4261 4262 279.3 473 bags20f08 4263 4264 279.3 477 BaGS9C24 4265 4266 281.5 477 BaGS22O02 4267 4268 281.5 479 baak13k16 4269 4270 282.6 479 MWG2249 — — 282.6 479 bah41b17 4271 4272 282.6 482 bah63m11 4273 4274 283.7 482 BaGS28P07 4275 4276 283.7 482 bags1h15 4277 4278 283.7 482 kr26H0515 4279 4280 283.7 482 bags6n05 4281 4282 283.7 487 BaH24P17 4283 4284 284.1 488 bast03D0408 4285 4286 284.8 488 BaAL17G06 4287 4288 284.8 488 BaH32G11 4289 4290 284.8 488 BaGS4P17 4291 4292 284.8 488 baal31o21 4293 4294 284.8 488 BaGS36A16 4295 4296 284.8 488 bags4d11 4297 4298 284.8 488 basd13n18 4299 4300 284.8 496 basd0a08 4301 4302 285.2 497 BaGS32P24 4303 4304 285.9 498 baak12i02 4305 4306 288.2 498 kr06H0315 4307 4308 288.2 498 BaAL26B09 4309 4310 288.2

TABLE 5-11 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 498 kr32C11O5 4311 4312 288.2 498 BaH53B15 4313 4314 288.2 503 bags13hO4 4315 4316 291.4 504 baal15i11 4317 4318 295.7 505 bags1m19 4319 4320 307.4 506 HVM6 — — 331.7

As shown in Table 6-1 to Table 6-7, the chromosomal order in barley 6H chromosome (distance from the short arm end of 6H chromosome) has been specified for 321 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 4321 through SEQ ID NO: 4962. The chromosomal order in barley 6H chromosome has also been specified for 6 known clones (MWG620, Bmac316, MWG2218, HVM31, Bmac40, and MWG897).

TABLE 6-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 MWG620 — — 0 2 basd14f03 4321 4322 12.1 2 BaH30E11 4323 4324 12.1 4 BaH59J05 4325 4326 12.6 5 BaAL39O22 4327 4328 13.1 6 baal16j24 4329 4330 14.2 7 BaAL30P12 4331 4332 15.3 7 BaGS21H12 4333 4334 15.3 9 BaAK15J05 4335 4336 16.4 9 BaGS9G15 4337 4338 16.4 9 BaAK41J04 4339 4340 16.4 12 baak43j24 4341 4342 16.9 13 bah26n19 4343 4344 17.4 13 Bmac316 — — 17.4 13 bags38a05 4345 4346 17.4 13 BaH43D06 4347 4348 17.4 17 bags9j07 4349 4350 19.6 17 baak31p11 4351 4352 19.6 19 BaGS17P19 4353 4354 20.7 20 baal32j05 4355 4356 23.6 20 baal16l16 4357 4358 23.6 22 baal6a11 4359 4360 24.4 22 bah55p06 4361 4362 24.4 22 bags8f03 4363 4364 24.4 22 bags3m02 4365 4366 24.4 22 BaAL36L08 4367 4368 24.4 22 baet19E0309 4369 4370 24.4 22 BaSD18J06 4371 4372 24.4 29 bast48D0307 4373 4374 28.5 30 kr55D0707 4375 4376 29.8 30 BaAL4B09 4377 4378 29.8 30 bags32f21 4379 4380 29.8 33 BaGS24F04 4381 4382 31.4 33 BaGS10G07 4383 4384 31.4 35 BaGS26P07 4385 4386 31.9 35 bags12f11 4387 4388 31.9 35 MWG2218 — — 31.9 35 BaH63O04 4389 4390 31.9 39 BaGS26D21 4391 4392 32.3 40 bags20o14 4393 4394 33 41 baak36d08 4395 4396 34.1 41 bast75H0616 4397 4398 34.1 41 basd16g17 4399 4400 34.1 41 baal4k16 4401 4402 34.1 41 bags32b14 4403 4404 34.1 46 baal4d01 4405 4406 35.2 46 baal7d23 4407 4408 35.2 46 BaH62C20 4409 4410 35.2 49 BaH21K13 4411 4412 36.3 49 BaAK14J02 4413 4414 36.3

TABLE 6-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 51 bah22c08 4415 4416 38.1 51 baak25d07 4417 4418 38.1 53 BaAK29M05 4419 4420 40.3 54 BaGS31I08 4421 4422 44.7 54 bah12n12 4423 4424 44.7 54 baal30o06 4425 4426 44.7 54 baak13n14 4427 4428 44.7 54 bags7a09 4429 4430 44.7 54 baak32l04 4431 4432 44.7 60 bah31o16 4433 4434 45.8 60 BaAL7C19 4435 4436 45.8 62 BaAK23F04 4437 4438 46.1 63 kr40D0707 4439 4440 46.8 64 basd0g08 4441 4442 47.9 64 BaAL30H03 4443 4444 47.9 66 BaAL21B21 4445 4446 50.9 66 BaGS30E05 4447 4448 50.9 68 BaAK13G04 4449 4450 52.9 69 BaGS27N11 4451 4452 55.8 70 BaGS9A04 4453 4454 62.5 71 BaAK23M11 4455 4456 68.4 72 basd3f22 4457 4458 70.5 73 baak41p21 4459 4460 71.6 73 BaAK41K07 4461 4462 71.6 73 BaH53I11 4463 4464 71.6 73 baak26i14 4465 4466 71.6 73 BaAL18N03 4467 4468 71.6 73 baak40c08 4469 4470 71.6 73 bags17l19 4471 4472 71.6 73 BaSD25N02 4473 4474 71.6 81 BaAK37E04 4475 4476 73.8 82 baal33h17 4477 4478 75.4 83 bags20d08 4479 4480 77 84 BaGS4J01 4481 4482 78.1 84 bah48o03 4483 4484 78.1 84 bastl31A0901 4485 4486 78.1 84 bah20k22 4487 4488 78.1 84 BaGS15J17 4489 4490 78.1 84 BaAK17I01 4491 4492 78.1 84 BaAK30F08 4493 4494 78.1 84 bah47a17 4495 4496 78.1 84 basd15e10 4497 4498 78.1 93 BaH56C11 4499 4500 79.2 93 BaH18C17 4501 4502 79.2 93 BaGS20G24 4503 4504 79.2 96 bags19g17 4505 4506 81.5 97 BaAK21L02 4507 4508 84.9 98 BaH52H21 4509 4510 89.2 99 bags9l13 4511 4512 90.2 99 BaSD18C12 4513 4514 90.2

TABLE 6-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 101 baak23k07 4515 4516 91.1 102 bags16j16 4517 4518 91.9 103 BaSD15O17 4519 4520 93.1 104 baak12i20 4521 4522 93.6 104 BaAK3M04 4523 4524 93.6 104 BaGS4N23 4525 4526 93.6 104 baak34k24 4527 4528 93.6 104 bah15n23 4529 4530 93.6 104 baak45i02 4531 4532 93.6 104 basd13i08 4533 4534 93.6 111 BaSD17P01 4535 4536 94.7 111 HVM31 — — 94.7 111 baak13d19 4537 4538 94.7 111 baal3h14 4539 4540 94.7 111 bags33j18 4541 4542 94.7 111 BaH45B01 4543 4544 94.7 111 baak21n07 4545 4546 94.7 111 BaH36H18 4547 4548 94.7 119 BaAL23O14 4549 4550 95.8 120 bah53f05 4551 4552 99.1 121 baak12d02 4553 4554 100.2 122 bags11h12 4555 4556 101.8 122 baet01F1212 4557 4558 101.8 124 baak17l11 4559 4560 102.3 124 basd20e17 4561 4562 102.3 124 bah29p24 4563 4564 102.3 124 bags30o05 4565 4566 102.3 124 kr68D0208 4567 4568 102.3 124 bags37l11 4569 4570 102.3 124 baal4d14 4571 4572 102.3 124 BaH58I23 4573 4574 102.3 124 bags39l04 4575 4576 102.3 124 baak29i13 4577 4578 102.3 124 bags20p18 4579 4580 102.3 124 BaAK12J13 4581 4582 102.3 124 BaH13K17 4583 4584 102.3 124 bah60p09 4585 4586 102.3 124 BaH27N11 4587 4588 102.3 124 BaGS34D11 4589 4590 102.3 124 BaGS39G07 4591 4592 102.3 124 bah22o14 4593 4594 102.3 124 bah14l20 4595 4596 102.3 124 bah42p22 4597 4598 102.3 124 BaAK21G03 4599 4600 102.3 124 BaAL35D24 4601 4602 102.3 124 baak45h14 4603 4604 102.3 124 bags28o05 4605 4606 102.3 124 BaAK31P07 4607 4608 102.3 124 bah36c06 4609 4610 102.3 124 BaGS37H24 4611 4612 102.3

TABLE 6-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 124 bast65B0303 4613 4614 102.3 124 BaH51A22 4615 4616 102.3 153 BaGS39K23 4617 4618 103.4 153 BaSD14O12 4619 4620 103.4 153 BaH57F12 4621 4622 103.4 153 BaGS32G02 4623 4624 103.4 153 BaAL40N06 4625 4626 103.4 153 bah11b14 4627 4628 103.4 153 bah15j14 4629 4630 103.4 153 bast21C1105 4631 4632 103.4 153 bags29f03 4633 4634 103.4 153 BaAK46B16 4635 4636 103.4 153 basd11p22 4637 4638 103.4 153 baal16h16 4639 4640 103.4 165 BaSD21L07 4641 4642 104.9 165 BaH42D07 4643 4644 104.9 167 BaGS23K24 4645 4646 106.4 167 BaAK24L01 4647 4648 106.4 167 BaAL11H22 4649 4650 106.4 167 BaAL13O24 4651 4652 106.4 167 BaAL6M22 4653 4654 106.4 167 BaH56I06 4655 4656 106.4 167 BaSD19A18 4657 4658 106.4 174 BaSD20P03 4659 4660 108.2 174 BaH19B13 4661 4662 108.2 176 kr59H0416 4663 4664 110.7 177 BaGS14A02 4665 4666 112.2 177 BaH34P05 4667 4668 112.2 177 bah61o16 4669 4670 112.2 177 BaH24N07 4671 4672 112.2 177 BaAL20M22 4673 4674 112.2 177 BaAL11H20 4675 4676 112.2 177 bah54b04 4677 4678 112.2 177 BaAK14H23 4679 4680 112.2 177 BaSD20M23 4681 4682 112.2 177 BaAL15B12 4683 4684 112.2 177 baal15d09 4685 4686 112.2 177 baak39n20 4687 4688 112.2 177 BaH43N16 4689 4690 112.2 177 bags39h18 4691 4692 112.2 177 BaAL3L23 4693 4694 112.2 192 basd22c03 4695 4696 115.6 193 bast55G0913 4697 4698 117.9 193 kr47F0511 4699 4700 117.9 195 bah15l24 4701 4702 118.4 196 bast77A0402 4703 4704 118.9 196 BaH58F04 4705 4706 118.9 196 BaAK30L09 4707 4708 118.9 196 kr39G1113 4709 4710 118.9 196 baak1c16 4711 4712 118.9

TABLE 6-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 196 basd20e05 4713 4714 118.9 196 BaAK12F18 4715 4716 118.9 196 BaH50L11 4717 4718 118.9 196 bast50G0814 4719 4720 118.9 196 bast70D0107 4721 4722 118.9 196 baal17d05 4723 4724 118.9 196 baak46g03 4725 4726 118.9 196 bah63m17 4727 4728 118.9 209 BaH15L15 4729 4730 120 209 BaGS19N01 4731 4732 120 209 BaAK32P07 4733 4734 120 212 bags14l13 4735 4736 121.1 213 bags37o24 4737 4738 121.7 214 baal5e24 4739 4740 122.8 215 BaH46B06 4741 4742 124.5 215 bags11a16 4743 4744 124.5 215 baal27m03 4745 4746 124.5 215 BaAK1C18 4747 4748 124.5 215 BaAK38H16 4749 4750 124.5 215 BaGS37K02 4751 4752 124.5 221 bah28a10 4753 4754 125.6 221 bah27c03 4755 4756 125.6 221 BaH52B11 4757 4758 125.6 224 bah27f05 4759 4760 127.8 224 bags37g15 4761 4762 127.8 224 bah59c10 4763 4764 127.8 224 bags38j06 4765 4766 127.8 224 basd14e01 4767 4768 127.8 224 BaGS24L06 4769 4770 127.8 224 bags17b02 4771 4772 127.8 224 BaH54L03 4773 4774 127.8 224 BaAK21J17 4775 4776 127.8 233 BaGS23G08 4777 4778 128.9 233 basd18e06 4779 4780 128.9 233 baak19o02 4781 4782 128.9 233 basd2o16 4783 4784 128.9 237 baak16e08 4785 4786 130 237 bags18h01 4787 4788 130 239 BaGS4L20 4789 4790 133.4 240 BaAL2M19 4791 4792 135.8 241 BaH36F15 4793 4794 139.1 242 bah58l07 4795 4796 140.2 243 baet29C0406 4797 4798 141.3 243 BaAK35B04 4799 4800 141.3 243 bags34k13 4801 4802 141.3 246 baal29i08 4803 4804 142.1 246 baak11p10 4805 4806 142.1 248 BaH30J08 4807 4808 145.1 249 bags4e12 4809 4810 147.3 250 bastl05C1206 4811 4812 148.4

TABLE 6-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 250 bast22G0313 4813 4814 148.4 252 basd24b08 4815 4816 149.5 252 bastl16C0206 4817 4818 149.5 252 bags33n21 4819 4820 149.5 255 baak33c11 4821 4822 151.7 255 baal13o10 4823 4824 151.7 257 BaH36P06 4825 4826 154.9 258 basd12l01 4827 4828 157.1 259 baet25H0115 4829 4830 158.2 259 bags15d14 4831 4832 158.2 259 BaGS37E09 4833 4834 158.2 259 BaAK29I15 4835 4836 158.2 259 BaAK18I01 4837 4838 158.2 264 bast62B0404 4839 4840 160.4 265 bah25l03 4841 4842 163.9 266 BaGS19I11 4843 4844 167.2 266 baal1n11 4845 4846 167.2 268 baak16l07 4847 4848 167.6 268 BaGS18P04 4849 4850 167.6 270 bags19e06 4851 4852 168.9 270 baak35n07 4853 4854 168.9 272 BaAK29K23 4855 4856 173 272 baal13c18 4857 4858 173 272 bags7d17 4859 4860 173 275 bags20j08 4861 4862 174.1 275 baal9c20 4863 4864 174.1 277 bags20l05 4865 4866 175.2 278 bah63k05 4867 4868 176.3 279 BaAK14C17 4869 4870 178.5 280 Bmac40 — — 179.6 280 baal15i23 4871 4872 179.6 282 BaAK1N06 4873 4874 181.7 282 bags38d10 4875 4876 181.7 282 BaAK19J15 4877 4878 181.7 285 BaH50L14 4879 4880 182.8 285 BaGS37E11 4881 4882 182.8 287 BaSD27E20 4883 4884 183 288 basd17f18 4885 4886 184.1 289 baal32m04 4887 4888 188.5 290 BaSD12L12 4889 4890 191.7 291 BaGS37D24 4891 4892 194 291 BaGS9K15 4893 4894 194 293 BaGS33L03 4895 4896 196 293 BaAK24G10 4897 4898 196 295 kr58F0511 4899 4900 196.5 296 BaH50F16 4901 4902 197 296 basd17d11 4903 4904 197 298 BaH37P24 4905 4906 198.1 298 bah47l21 4907 4908 198.1 298 baet45C1105 4909 4910 198.1

TABLE 6-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 301 baak39o18 4911 4912 199.2 301 bags20p12 4913 4914 199.2 301 bags38f23 4915 4916 199.2 304 baak14b08 4917 4918 201.4 304 bah54n10 4919 4920 201.4 306 bags3k09 4921 4922 202.5 307 bags34p08 4923 4924 203.6 308 baal1e06 4925 4926 204.7 308 baak34p14 4927 4928 204.7 308 baak14k12 4929 4930 204.7 308 BaSD13G17 4931 4932 204.7 308 MWG897 — — 204.7 308 bags27h05 4933 4934 204.7 308 basd15m11 4935 4936 204.7 308 BaH28G09 4937 4938 204.7 308 bags21b06 4939 4940 204.7 308 basd12g17 4941 4942 204.7 318 BaH18F07 4943 4944 205 319 BaH44A23 4945 4946 205.3 320 bags3d07 4947 4948 205.6 321 BaAL6A21 4949 4950 205.9 321 BaAK24E07 4951 4952 205.9 321 BaAL29L09 4953 4954 205.9 321 kr66D1107 4955 4956 205.9 321 bastl47C0606 4957 4958 205.9 321 BaH50M03 4959 4960 205.9 327 basd27k17 4961 4962 207.3

As shown in Table 7-1 to Table 7-9, the chromosomal order in barley 7H chromosome (distance from the short arm end of 7H chromosome) has been specified for 409 clones including the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 4963 through SEQ ID NO: 5780. The chromosomal order in barley 7H chromosome has also been specified for 16 known clones (HVM4, HVCMA, cMWG704, MWG511, Bmag359, MWG2031, KT3, KT9, MWG975, EBmac764, Bmac0064, Bmag120, Bmac156, HVM49, MWG2062, and HVM5).

TABLE 7-1 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 1 basd2p19 4963 4964 0 2 BaAK38B18 4965 4966 4.7 3 bah42i06 4967 4968 6.6 3 bags6f02 4969 4970 6.6 5 BaAK39J07 4971 4972 7.2 5 BaAK1L23 4973 4974 7.2 5 baak17e10 4975 4976 7.2 5 BaH50H08 4977 4978 7.2 5 baal17e10 4979 4980 7.2 5 BaSD17C05 4981 4982 7.2 11 BaGS14P02 4983 4984 8.3 11 bastl42H0115 4985 4986 8.3 11 BaAK19C08 4987 4988 8.3 11 bastl40H1115 4989 4990 8.3 15 bags12l21 4991 4992 9.4 15 baak12j24 4993 4994 9.4 15 bags14d22 4995 4996 9.4 15 BaAK21P12 4997 4998 9.4 15 baal6b02 4999 5000 9.4 15 baak29f13 5001 5002 9.4 21 bast79C0406 5003 5004 11.3 22 BaH45M08 5005 5006 12.4 23 kr28A0301 5007 5008 16.4 23 bags19e04 5009 5010 16.4 25 BaH28L07 5011 5012 18.6 26 baak21m18 5013 5014 20.9 26 baak21l22 5015 5016 20.9 28 basd15h01 5017 5018 25.2 28 BaH19O11 5019 5020 25.2 28 BaH59A20 5021 5022 25.2 31 bags39l05 5023 5024 27.3 31 BaAK31N06 5025 5026 27.3 33 BaH58P03 5027 5028 28.4 34 bags29c18 5029 5030 29.5 35 BaAL4O04 5031 5032 30.6 35 bags37n23 5033 5034 30.6 35 BaAL39N03 5035 5036 30.6 38 BaAK14C23 5037 5038 31.7 38 kr61A1101 5039 5040 31.7 38 BaH42J17 5041 5042 31.7 41 BaH58H08 5043 5044 39.2 42 bast78A0202 5045 5046 40.4 43 BaGS21E20 5047 5048 42.5 43 bags22k18 5049 5050 42.5 45 BaGS33H15 5051 5052 45.7 45 HVM4 — — 45.7 47 BaSD18O16 5053 5054 47.9 47 baak22j11 5055 5056 47.9 49 BaAK20K04 5057 5058 50.4 50 bags29l11 5059 5060 55.2

TABLE 7-2 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 51 BaAK17G18 5061 5062 60.3 52 baak14o13 5063 5064 61.7 53 baal8d17 5065 5066 63.2 54 BaAK37P20 5067 5068 64.2 55 bags28k04 5069 5070 71.5 56 bah34b22 5071 5072 73.8 57 BaGS37M03 5073 5074 78.3 58 BaGS18C14 5075 5076 79.5 59 bast23G0214 5077 5078 80.6 59 bah47p02 5079 5080 80.6 61 baak42a24 5081 5082 81.7 61 baak16f06 5083 5084 81.7 61 bah11m12 5085 5086 81.7 64 kr66H0216 5087 5088 84.9 64 BaGS23D15 5089 5090 84.9 64 baal22c17 5091 5092 84.9 67 baak33d06 5093 5094 86 67 bags34l17 5095 5096 86 67 BaGS25G03 5097 5098 86 67 bah14j16 5099 5100 86 67 bah34a23 5101 5102 86 67 BaAK21B17 5103 5104 86 67 bast42C0406 5105 5106 86 74 baak21m13 5107 5108 87.3 75 baet45H0115 5109 5110 87.7 76 BaH38E01 5111 5112 88.1 76 bast03G0313 5113 5114 88.1 78 baak38p18 5115 5116 89.5 79 basd18g06 5117 5118 90.6 80 baak40i22 5119 5120 92 80 baal2n12 5121 5122 92 82 BaSD3C24 5123 5124 94.1 83 BaAK46M07 5125 5126 96.3 84 BaGS17N04 5127 5128 99.6 85 bags12d09 5129 5130 100.7 85 basd20c08 5131 5132 100.7 87 bags17i04 5133 5134 101.7 87 baak15p03 5135 5136 101.7 89 baak24b20 5137 5138 104.9 89 baal18g11 5139 5140 104.9 89 baal40b06 5141 5142 104.9 89 baak40g02 5143 5144 104.9 93 baal1m11 5145 5146 106.1 94 BaH26B16 5147 5148 107.9 94 baal5n08 5149 5150 107.9 96 BaSD27F05 5151 5152 109.5 96 bags9c08 5153 5154 109.5 96 baak3c01 5155 5156 109.5 99 basd1j22 5157 5158 110.6 99 BaH23N03 5159 5160 110.6

TABLE 7-3 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 99 bast46D0808 5161 5162 110.6 99 bast22A0802 5163 5164 110.6 99 bast34D0808 5165 5166 110.6 104 bast48E0509 5167 5168 114.9 104 bast26F1012 5169 5170 114.9 106 baal30e07 5171 5172 115.5 107 bags23n06 5173 5174 116.1 108 BaGS18I05 5175 5176 116.6 109 BaH52L11 5177 5178 116.8 110 baal18b16 5179 5180 117 111 bags1a17 5181 5182 118.1 111 HVCMA — — 118.1 111 bags8o06 5183 5184 118.1 111 baak46e14 5185 5186 118.1 115 BaGS38N08 5187 5188 118.6 115 BaH49P17 5189 5190 118.6 117 bah60l22 5191 5192 120.2 117 bags21m22 5193 5194 120.2 117 BaH54E07 5195 5196 120.2 120 BaAK39A20 5197 5198 121.3 121 bags21d11 5199 5200 123.5 122 kr39H0816 5201 5202 125.6 123 baet32B1103 5203 5204 131.1 124 BaAK31O16 5205 5206 132.1 124 bags39l20 5207 5208 132.1 124 BaGS15L12 5209 5210 132.1 127 kr67D0208 5211 5212 134.3 128 BaAK1H16 5213 5214 135.4 128 BaH26F10 5215 5216 135.4 130 bags14n02 5217 5218 135.9 131 bah36f01 5219 5220 136.4 132 bast79F0711 5221 5222 137.5 132 BaGS29G21 5223 5224 137.5 134 bast79D1107 5225 5226 139.6 134 BaGS26O20 5227 5228 139.6 134 bags11h08 5229 5230 139.6 134 bags22h11 5231 5232 139.6 134 baet19D0608 5233 5234 139.6 139 bags23a14 5235 5236 140.7 139 cMWG704 — — 140.7 139 bah62d14 5237 5238 140.7 142 BaGS37C09 5239 5240 142.6 142 bags20a01 5241 5242 142.6 144 bah53m11 5243 5244 144.5 144 BaGS26L01 5245 5246 144.5 144 bags21p23 5247 5248 144.5 144 BaAK24B01 5249 5250 144.5 144 BaAK13L03 5251 5252 144.5 149 MWG511 — — 145.6 149 bastl56C0105 5253 5254 145.6

TABLE 7-4 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 149 basd20j22 5255 5256 145.6 149 bags28d11 5257 5258 145.6 153 BaGS4I11 5259 5260 146.7 153 BaH52G15 5261 5262 146.7 153 baak11d12 5263 5264 146.7 153 BaH42A07 5265 5266 146.7 157 BaGS38F01 5267 5268 147.2 158 BaAK32I05 5269 5270 147.7 158 bah60f18 5271 5272 147.7 160 baal2n10 5273 5274 148.8 161 BaAK14F01 5275 5276 149.9 161 bah57o01 5277 5278 149.9 161 basd12f05 5279 5280 149.9 161 bah58e21 5281 5282 149.9 161 bags1d07 5283 5284 149.9 161 BaH23J15 5285 5286 149.9 161 baak1p09 5287 5288 149.9 168 BaAK32C23 5289 5290 151 168 baal9e06 5291 5292 151 168 baal18g23 5293 5294 151 168 bastl47E0309 5295 5296 151 168 baak30o08 5297 5298 151 168 baet44C0606 5299 5300 151 174 bags39g18 5301 5302 152.1 174 BaH50P14 5303 5304 152.1 176 bags38m06 5305 5306 152.6 177 baal33e12 5307 5308 153.2 177 bags3i04 5309 5310 153.2 179 BaAL1A11 5311 5312 153.7 179 BaSD2M23 5313 5314 153.7 181 Bmag359 — — 155.3 181 BaAK23L23 5315 5316 155.3 181 bags7a23 5317 5318 155.3 181 bah56l04 5319 5320 155.3 185 basd12k23 5321 5322 156.4 185 bast22C0105 5323 5324 156.4 185 BaGS38H20 5325 5326 156.4 185 bah61p21 5327 5328 156.4 185 basd11h13 5329 5330 156.4 185 BaAL41J12 5331 5332 156.4 185 baal0f04 5333 5334 156.4 185 kr18E0810 5335 5336 156.4 185 BaH15J07 5337 5338 156.4 185 BaAL6G08 5339 5340 156.4 185 BaGS24F02 5341 5342 156.4 185 baal19m12 5343 5344 156.4 185 bah34h16 5345 5346 156.4 185 baak2e06 5347 5348 156.4 185 bast10C0406 5349 5350 156.4 185 baak12l16 5351 5352 156.4

TABLE 7-5 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 185 baak29d01 5353 5354 156.4 185 BaAK19N06 5355 5356 156.4 185 BaAK4I02 5357 5358 156.4 185 BaH49K10 5359 5360 156.4 185 baal2o03 5361 5362 156.4 185 baak28g14 5363 5364 156.4 185 bah62n05 5365 5366 156.4 185 bags16e11 5367 5368 156.4 185 bags23g13 5369 5370 156.4 210 BaSD23C05 5371 5372 157 211 BaH19F17 5373 5374 157.4 211 BaH15J02 5375 5376 157.4 211 baak20d11 5377 5378 157.4 211 BaAL6D19 5379 5380 157.4 211 baak43e16 5381 5382 157.4 216 baak40b02 5383 5384 158.5 217 bah62m03 5385 5386 159.6 218 BaGS26G17 5387 5388 161.8 219 baal6j16 5389 5390 162.9 219 bags29b09 5391 5392 162.9 219 baal20e03 5393 5394 162.9 219 BaGS9L23 5395 5396 162.9 219 bah32e22 5397 5398 162.9 219 BaAK17O14 5399 5400 162.9 225 bah56g23 5401 5402 164 225 basd22f14 5403 5404 164 227 BaH42O04 5405 5406 164.7 227 bast74F0111 5407 5408 164.7 229 BaSD21F13 5409 5410 167.3 230 BaAK26G13 5411 5412 167.4 231 BaAK44D07 5413 5414 168.2 231 bast61C0206 5415 5416 168.2 233 BaAL29L08 5417 5418 169.4 233 BaH34B20 5419 5420 169.4 233 baak21a04 5421 5422 169.4 233 BaGS37F18 5423 5424 169.4 233 baal12h12 5425 5426 169.4 233 BaAK42B16 5427 5428 169.4 233 BaAK26F12 5429 5430 169.4 233 BaH27B21 5431 5432 169.4 233 BaGS12B05 5433 5434 169.4 233 baal12h24 5435 5436 169.4 243 baal35j16 5437 5438 173.5 243 BaGS37C07 5439 5440 173.5 243 BaAK45D23 5441 5442 173.5 243 BaH42G09 5443 5444 173.5 243 BaAL40P01 5445 5446 173.5 243 kr61A0901 5447 5448 173.5 249 baal4d05 5449 5450 174.6 249 basd20l03 5451 5452 174.6

TABLE 7-6 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 249 baal31b03 5453 5454 174.6 252 bags27k21 5455 5456 176.8 252 bah55n15 5457 5458 176.8 254 BaH44B17 5459 5460 177.9 254 BaSD2K11 5461 5462 177.9 256 BaAK20B23 5463 5464 180.1 256 BaGS23E20 5465 5466 180.1 256 bags33i10 5467 5468 180.1 256 bags35k06 5469 5470 180.1 260 bags22c18 5471 5472 181.2 260 MWG2031 — — 181.2 260 BaGS22M17 5473 5474 181.2 263 baak21j24 5475 5476 184.4 263 BaH29D20 5477 5478 184.4 263 bah44f20 5479 5480 184.4 263 BaAL16O08 5481 5482 184.4 263 BaAL27L17 5483 5484 184.4 263 sKT3 — — 184.4 263 sKT9 — — 184.4 263 baet31B1103 5485 5486 184.4 263 MWG975 — — 184.4 272 BaAL4A11 5487 5488 185.9 272 BaGS22I18 5489 5490 185.9 274 BaGS24K08 5491 5492 186.4 274 bah16c17 5493 5494 186.4 274 bah42m04 5495 5496 186.4 274 bags28l21 5497 5498 186.4 274 basd12f23 5499 5500 186.4 274 bah31m22 5501 5502 186.4 274 BaAL30C17 5503 5504 186.4 274 BaAK40P18 5505 5506 186.4 274 baak37n01 5507 5508 186.4 274 BaGS24O11 5509 5510 186.4 274 BaH27K19 5511 5512 186.4 274 BaGS15J07 5513 5514 186.4 286 bah19a07 5515 5516 188.9 287 BaH50I23 5517 5518 194 288 BaH56D06 5519 5520 195 288 BaAK39M05 5521 5522 195 288 bah11m16 5523 5524 195 291 bah49d03 5525 5526 196.1 291 bah62l23 5527 5528 196.1 291 BaAK38J13 5529 5530 196.1 291 BaH37N04 5531 5532 196.1 291 basd27c06 5533 5534 196.1 291 bah39g10 5535 5536 196.1 291 BaSD18P05 5537 5538 196.1 298 BaGS20A13 5539 5540 196.6 299 bah44n05 5541 5542 197.2 300 EBmac764 — — 198.2

TABLE 7-7 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 301 BaH27L15 5543 5544 199.3 301 BaAK21I02 5545 5546 199.3 303 Bmac0064 — — 200.3 304 BaH59F07 5547 5548 210.4 304 bags9l05 5549 5550 210.4 306 BaGS13H12 5551 5552 211.7 307 BaAL19B06 5553 5554 212.9 308 BaSD15P23 5555 5556 215.1 309 bah53j21 5557 5558 217.2 309 bah21f16 5559 5560 217.2 309 baet39E0309 5561 5562 217.2 312 bags5l04 5563 5564 217.7 313 bags7g10 5565 5566 218.3 314 BaGS9D22 5567 5568 219.5 315 kr27H1216 5569 5570 221.2 315 BaAK25O11 5571 5572 221.2 317 bah18j14 5573 5574 221.7 317 bags37k14 5575 5576 221.7 317 baak26n19 5577 5578 221.7 317 bah42k03 5579 5580 221.7 317 BaH36N15 5581 5582 221.7 317 Bmag120 — — 221.7 323 bags12i02 5583 5584 225 323 BaAK38E05 5585 5586 225 325 baet25B0604 5587 5588 226.1 325 BaAK46E21 5589 5590 226.1 327 BaGS37J12 5591 5592 229.9 328 bast60C0105 5593 5594 231 328 baak30b08 5595 5596 231 328 BaAL29N13 5597 5598 231 328 BaAL24O04 5599 5600 231 332 baak20o12 5601 5602 232.1 332 basd14k23 5603 5604 232.1 332 BaAK23L05 5605 5606 232.1 332 BaGS31G02 5607 5608 232.1 332 bags33o01 5609 5610 232.1 332 basd18g14 5611 5612 232.1 332 bah24d24 5613 5614 232.1 332 BaGS12F09 5615 5616 232.1 332 BaAK33H23 5617 5618 232.1 332 bah47p03 5619 5620 232.1 342 BaSD14F09 5621 5622 232.3 343 bah11k13 5623 5624 232.5 343 BaSD22F10 5625 5626 232.5 345 bags32a01 5627 5628 234 346 bags27o20 5629 5630 235.1 347 baak31o10 5631 5632 236.2 347 bags10f16 5633 5634 236.2 347 baal37j12 5635 5636 236.2 350 BaGS9H02 5637 5638 239.5

TABLE 7-8 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 351 BaAK40H10 5639 5640 240.3 352 bah56m09 5641 5542 240.6 353 kr66C0705 5643 5644 240.9 354 BaGS33M05 5645 5646 242 355 bast60B0204 5647 5648 243.2 356 bah56p08 5649 5650 245.4 357 baak12e19 5651 5652 246.9 357 BaGS1D05 5653 5654 246.9 359 BaH29M21 5655 5656 247.6 359 bags21c04 5657 5658 247.6 361 baak32p09 5659 5660 250.9 362 BaAL24B02 5661 5662 254.2 362 BaGS34F08 5663 5664 254.2 364 bast36D1208 5665 5666 255.3 364 kr25B1103 5667 5668 255.3 366 kr08D0303 5669 5670 256.4 367 baal19g05 5671 5672 257.5 367 BaH22B15 5673 5674 257.5 367 bags19c10 5675 5676 257.5 370 bags29k01 5677 5678 260.7 370 bastl54G0414 5679 5680 260.7 370 BaGS23J01 5681 5682 260.7 370 bags34b15 5683 5684 260.7 374 baak46i07 5685 5686 262.9 375 BaAL6J13 5687 5688 265 375 bah31l17 5689 5690 265 375 BaAK32A05 5691 5692 265 375 BaGS30N22 5693 5694 265 379 BaH54J20 5695 5696 266.4 380 BaGS7F20 5697 5698 269.4 381 bah63p18 5699 5700 272.8 382 bags11p11 5701 5702 274.9 383 BaH27L17 5703 5704 277.1 384 basd17e11 5705 5706 278.2 384 bags15i06 5707 5708 278.2 384 BaAK36F09 5709 5710 278.2 384 bast57A0101 5711 5712 278.2 388 baak26h17 5713 5714 280.3 388 basd13b15 5715 5716 280.3 388 bags9d11 5717 5718 280.3 391 BaH20K03 5719 5720 281.4 391 BaSD27N21 5721 5722 281.4 391 baal13a10 5723 5724 281.4 394 kr30F1212 5725 5726 282.5 394 bastl45B0903 5727 5728 282.5 394 Bmac156 — — 282.5 394 bah60k06 5729 5730 282.5 398 BaGS32I12 5731 5732 283.6 398 BaGS4H02 5733 5734 283.6 398 bah44k12 5735 5736 283.6

TABLE 7-9 Order from SEQ ID SEQ ID Distance from the short NO: NO: the short arm end Clones (5′ end) (3′ end) arm end (cM) 398 bags21m21 5739 5740 283.6 398 BaSD14J15 5741 5742 283.6 404 HVM49 — — 285.7 404 basd16e11 5743 5744 285.7 406 BaAK21N09 5745 5746 287.9 407 baal0e10 5747 5748 289 408 baak1g17 5749 5750 290.1 409 BaH14K12 5751 5752 291.2 409 BaAK31G05 5753 5754 291.2 411 MWG2062 — — 291.5 412 BaH50G17 5755 5756 292.3 413 HVM5 — — 293.4 413 bags38n21 5757 5758 293.4 413 bastl04B0303 5759 5760 293.4 413 baal35f12 5761 5762 293.4 417 BaH49M02 5763 5764 293.9 418 baak38f04 5765 5766 294.5 419 bah29b02 5767 5768 295 420 basd15d20 5769 5770 296.1 421 kr61C0305 5771 5772 297.1 421 BaH23B08 5773 5774 297.1 423 kr40H1115 5775 5776 298.6 424 bah51g17 5777 5778 301.4 425 BaSD22E19 5779 5780 315.4

The order on each chromosome can be specified based on the distance from the short arm end or long arm end. In the case where the order on each chromosome was specified by the distance from the short arm end, the polynucleotides mapped on 1H chromosome are first placed based on the distance from the short end, and the polynucleotides mapped on 2H and the subsequent chromosomes (3H to 7H) are placed based on the same reference (distances from the short arm end).

More specifically, in the case where the distance from the short arm end is used as a reference for positioning the polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, the polynucleotides are placed according to the order from the short arm end as shown in Table 1-1 to Table 7-9. For clones that have the same order from the short arm end, the order by which these clones are placed is not particularly limited. Once the precise order of these clones were specified by a future study, these clones will be able to be placed accordingly. Note that, the reference (origin) on the chromosome is not just limited to the short arm end of 1H chromosome. Any position on a chromosome can be used as a reference point.

When using more than one polynucleotide with base sequences from the base sequence of any one of SEQ ID NO: 1 through 5780 for example, the polynucleotides can be placed in order from the 5′ end. Further, in the case where a polynucleotide with the base sequence of any one of SEQ ID NO: 1 through 5780 is used together with more than one polynucleotide with base sequences from the base sequence of any one of SEQ ID NO: 1 through 5780 for example, a polynucleotide that is closest to the 5′ end is placed first. The same criteria can be used to place other polynucleotides as well.

With the polynucleotides immobilized on a support in the order they are arranged on the chromosomes, the value of a gene detection instrument according to the present invention can be greatly improved in crossbreeding of Triticeae species. More specifically, by using a gene detection instrument according to the present invention for comprehensive investigation of transcripts in hybrids of Triticeae species, the expression level or the presence or absence of polymorphism can be determined for target genes. Further, the location and extent of recombination on chromosomes can be checked to see if unnecessary recombination has occurred. This makes it easy to determine target individuals of screening and thereby improves the efficiency of breeding.

[Gene Detection Instrument with the Polynucleotides Immobilized in Regions Appended with Chromosomal Order Information]

The regions in which the polynucleotides are immobilized (hereinafter, may be referred to as “spots”) may be appended with information indicative of the order in which the polynucleotides are arranged on barley chromosomes. The spots may be arranged in any way as long as they are appended with the order information. With the order information added to the spots, the data obtained from the spots can be rearranged in the chromosomal order even when the spots are randomly placed on a support. In this way, a gene detection instrument according to the present invention can improve the efficiency of breeding. Note that, the order information added to the spots can follow the foregoing criteria used to place the polynucleotides.

Adding order information enables the spots to be arranged in an arbitrary order in a gene detection instrument of an array type, in which more than one polynucleotide is immobilized on a support such as a membrane or a glass slide. Further, the chromosomal order information of individual polynucleotides can also be added in a gene detection instrument that employs a collection of beads (bead array) in which the polynucleotide is immobilized on each bead serving as a support.

[Examples of a Gene Detection Instrument According to the Present Invention]

(i) For Detecting Gene Expression

A gene detection instrument according to the present invention can be fabricated according to a conventional gene expression array fabrication method. For example, a cDNA array can be fabricated by preparing a solution of polynucleotide that comprises cDNA (full length or partial), and by spotting it on a support with a spotteror the like. The polynucleotides immobilized on the support may be synthetic oligonucleotides with the base sequences of cDNA. Alternatively, a technique known as the Affymetrix DNA chip technique may be used, in which DNA is synthesized on a substrate.

Various conventional techniques that are designed for detection of gene expression can suitably be used in a gene detecting instrument according to the present invention. For example, cDNA or cRNA prepared from total RNA or mRNA of Triticeae species can be used for the present invention. More specifically, cDNA or cRNA is fluorescent-labeled and hybridized with the polynucleotides immobilized on the support. The expression level of genes can be evaluated by measuring the intensity of hybridization, using the fluorescence as an index. Further, cDNA or cRNA prepared from two kinds of samples may be labeled with fluorescent substances that emit different colors, and hybridized with polynucleotides immobilized on the same support. By measuring the color tone and fluorescent intensity, differences of gene expression can be evaluated.

In breeding of Triticeae species, target individuals can be screened for according to changes in the expression levels of genes that regulate target traits. Further, target individuals can also be screened for according to the expression levels of genes that are linked to target genes.

(ii) For Detecting Gene Polymorphism

Various conventional techniques that are designed for detection of gene polymorphism can suitably be used in a gene detection instrument according to the present invention.

For example, a gene detection instrument according to the present invention can be used to detect fragment length polymorphism such as RFLP (restriction fragment length polymorphism). More specifically, fragment length polymorphism can be detected by immobilizing more than one partial sequence of cDNA of the same clone on the same spot. Here, the hybridization intensity of the spot immobilizing three partial sequences is the strongest in fragments that hybridize with all of the three partial sequences, and is weaker in fragments that hybridize with two partial sequences or only one partial sequence. Thus, fragment length polymorphism can be detected by measuring fluorescence intensity or the like used as an index of hybridization intensity. In the case where fragments hybridize with distinctively different regions in the same spot, fragment length polymorphism can be detected by labeling the two fragments with different fluorescent dyes.

(2) Gene Polymorphism Detection Instrument according to the Present Invention

A gene polymorphism detection instrument according to the present invention is an instrument for detecting polymorphisms of genes in the genomes of Triticeae species. The organisms to which a gene detection instrument of the invention is applicable may be any Triticeae species, among which barley, wheat, and rye are preferable. A gene detection instrument according to the present invention includes a support on which polynucleotides constituting part of barley chromosomal (1H, 2H, 3H, 4H, 5H, 6H, and 7H) DNA are immobilized. The polynucleotides immobilized on the support may solely be polynucleotides that constitute part of barley chromosomal DNA, or other polynucleotides may additionally be immobilized on the support. Such additional polynucleotides are not particularly limited as long as they can detect expression or polymorphism of genes in the genomes of Triticeae species. For example, the additional polynucleotides may be those with the base sequences originating in non-barley organisms, or those with arbitrary base sequences that have been artificially synthesized.

In the case where the polynucleotides are immobilized in more than one region of the support, the polynucleotides immobilized in these regions may have non-overlapping base sequences or partially overlapping base sequences. Alternatively, polynucleotides of the same base sequence may be immobilized in these different regions of the support. In the case where the polynucleotides have overlapping base sequences, the polynucleotides may have partially overlapping base sequences, or the base sequence of one of the polynucleotides may be a partial sequence of the other polynucleotide.

Further, the polynucleotide immobilized in each region is not necessarily required to be of the same kind. More than one kind of polynucleotide may be immobilized in each region.

The support is not particularly limited as long as it can immobilize polynucleotides, and it may have any shape and may be made of any material. Examples of a support material generally include: inorganic materials such as glass and silicon wafer; natural polymers such as paper; synthetic polymers such as nitrocellulose and nylon; and gels using synthetic polymers or natural polymers. The shape of the support is not particularly limited as long as it provides enough area to support the polynucleotides. Generally, those with a two-dimensional plane, for example, such as a substrate with little or no flexibility, a flexible membrane, or a flexible substrate with intermediate flexibility can be preferably used. The thickness of the substrate or membrane is not particularly limited either, and it can be suitably set according to the material or use of the substrate or membrane. Various types of beads may be used as supports.

[Polynucleotides Immobilized on a Support of the Gene Polymorphism Detection Instrument]

In a gene polymorphism detection instrument according to the present invention, at least one polynucleotide from the following polynucleotides is immobilized on a support.

Polynucleotides with base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

As used herein, a polynucleotide with a base sequence constituting part of barley chromosomal DNA is not particularly limited as long as it is a polynucleotide with a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes.

A variant with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotide with a base sequence constituting part of barley chromosomal DNA may be a polynucleotide that has been mutated on purpose, or a polynucleotide that exists in nature. For example, think of a base sequence of chromosomal DNA in a specific variety of barley. Comparing this base sequence with those of other varieties, no sequence is completely identical. Rather, these sequences are variants with the substitution, deletion, insertion, and/or addition of one or more bases.

A polynucleotide immobilized on a support of a gene polymorphism detection instrument according to the present invention is preferably a polynucleotide that constitutes part of the base sequence of at least one DNA fragment that has been amplified with a primer set that comprises two primers arbitrarily selected from a plurality of primers that have been designed based on base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from the base sequences of SEQ ID NO: 1 through 5780, using the genomic DNA of Triticeae species as a template.

The base sequences of SEQ ID NO: 1 through 5780 are base sequences of the barley EST (expressed sequence tag) independently developed by the inventors. The inventors have previously confirmed that a polynucleotide with the base sequences of SEQ ID NO: 1 through 770, a polynucleotide with the base sequences of SEQ ID NO: 771 through 1754, a polynucleotide with the base sequences of SEQ ID NO: 1755 through 2642, a polynucleotide with the base sequences of SEQ ID NO: 2643 through 3324, a polynucleotide with the base sequences of SEQ ID NO: 3325 through 4320, a polynucleotide with the base sequences of SEQ ID NO: 4321 through 4962, and a polynucleotide with the base sequences of SEQ ID NO: 4963 through 5780 are mapped on 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes, respectively.

The primers are not particularly limited as long as they are designed based on the base sequences of SEQ ID NO: 1 through 5780. As such, primer profiles, such as the number of bases and GC content are not particularly limited. Further, as described above, since the base sequence of SEQ ID NO: n (where n is an odd number) and the base sequence of SEQ ID NO: n+1 are base sequences that are read from the both ends of cDNA of the same clone, the primer set with a combination of two primers arbitrarily selected from a plurality of primers that have been designed based on these base sequences flank a specific region of the barley chromosomal DNA. For example, in amplifying DNA with the primer set using the genomic DNA of barley as a template, the base sequences of the amplified fragments will be the same as the base sequences of the cDNA when the primer set is within the same exon. On the other hand, when the primers of the primer set are in different exons, the amplified fragments will include base sequences of the intron portions.

The primers that are designed based on the base sequences of SEQ ID NO: 1 through 5780 may be primers with the base sequences of SEQ ID NO: 5781 through 17340, or primers with the complementary sequences thereof, for example. More specifically, the primers that are designed based on SEQ ID NO: 1 are primers with the base sequences of SEQ ID NO: 5781 and 5782 or the complementary sequences thereof, and the primers that are designed based on SEQ ID NO: 2 are primers with the base sequences of SEQ ID NO: 5783 and 5784 or the complementary sequences thereof. In other words, the SEQ ID NOs for the base sequences of the primers designed based on the individual base sequences of SEQ ID NO: 1 through 5780 are represented by (2×m−1)+5780 and (2×m)+5780, where m is the SEQ ID NO of the base sequence used to designed the primer.

As a specific example, amplified DNA fragments can be obtained by PCR that uses the genomic—DNA of barley as a template and is performed with a primer set including a combination of two primers arbitrarily selected from (i) primers that have been designed based on the base sequence of SEQ ID NO: 1 and having the base sequences of SEQ ID NO: 5781 through 5782 or complementary sequences thereof, and (ii) primers that have been designed based on the base sequence of SEQ ID NO: 2 and having the base sequences of SEQ ID NO: 5783 through 5784 or complementary sequences thereof. A means by which amplification is performed is not particularly limited, and PCR and other conventional methods can be used.

The polynucleotides immobilized on a support of a gene polymorphism detection instrument according to the present invention are part of the amplified DNA fragments. When amplification is performed using genomic DNA of different Triticeae species as a template and when polymorphism exists in the amplified fragments, it is preferable that the polynucleotides immobilized on the support include polymorphism-containing portions of the base sequences. The polymorphism may be amplified fragment length polymorphism or single nucleotide polymorphism (SNP), for example.

The inventors of the present invention amplified DNA fragments by using genomic DNA of Triticeae species as a template and with the use of a primer set that included a combination of two primers arbitrarily selected from (i) primers that had been designed based on the base sequence of SEQ ID NO: n (where n is an odd number), and (ii) primers that had been designed based on the base sequence of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780. The inventors have found that these DNA fragments could be used as genetic markers for distinguishing different varieties of Triticeae species. That is, the inventors have specified at least 2890 genetic markers.

Specifically, the inventors have found fragment length polymorphism in the DNA fragments that were amplified by PCR that was performed with a primer set that had been designed based on SEQ ID NO: 2 and included a combination of primers with the base sequences of SEQ ID NO: 5783 and 5784, using the genomic DNA of malting barley “Haruna Nijo” (Hordeum vulgare ssp. vulgare variety Harunanijo) and the genomic DNA of wild type barley “H602” (Hordeum vulgare ssp. spontaneum H602) as templates. Further, the inventors have found SNP in the DNA fragments that were amplified by PCR that was performed with a primer set that had been designed based on SEQ ID NO: 4 and included a combination of primers with the base sequences of SEQ ID NO: 5787 and 5788, using the genomic DNA of the foregoing barley varieties as templates. It was found as a result that these DNA fragments with SNPs were CAPS (cleaved amplified polymorphic sequence) markers, which are excised by restriction enzyme in one of the varieties but not in the other varieties.

Table 8-1 through Table 14-9 show kinds of polymorphisms that were found by the inventors between Haruna Nijo and H602. Tables 8-1 through 8-8 show polymorphisms in 1H chromosome, Tables 9-1 through 9-10 in 2H chromosome, Tables 10-1 through 10-9 in 3H chromosome, Tables 11-1 through 11-7 in 4H chromosome, Tables 12-1 through 12-10 in 5H chromosome, Tables 13-1 through 13-7 in 6H chromosome, and Tables 14-1 through 14-9 in 7H chromosome. Corresponding EST sequences of the respective clones are shown in Table 1-1 through Table 7-9. The “Primers Used” are primers that were actually used from among the primers that had been designed based on the EST sequences (SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1) corresponding to the respective clones. The primers used for each clone had the base sequences with the SEQ ID NOs shown in the tables. The DNA fragments amplified with these primer sets using the genomic DNA of Haruna Nijo and the genomic DNA of H602 as templates had polymorphisms shown in Table 8-1 through Table 14-9.

The clones are known genetic markers, which have been identified as SSR (simple sequence repeat) marker, STS (sequence tagged site) marker, dCAPS (derived cleaved amplified polymorphic sequence) marker, trait marker, CAPS marker, or size_poly (fragment length polymorphism) marker. SSR is a fragment length polymorphism based on differences in the number of repeats of recurring units of two or three bases of DNA. STS is a sequence site that has been set only for a specific location of DNA. Starting from such a specific sequence site, specific DNA fragments are amplified by PCR or the like, and electrophoresis is run for the amplified products so that polymorphism can be detected based on the presence or absence of bands, or differences in molecular weight. In dCAPS, a mismatch is induced between PCR primers and template DNA, so that polymorphism (the presence or absence of restriction enzyme site) occurs in the PCR products. Trait marker sh is an isolated gene that controls vernalization requirement. Since Haruna Nijo and H602 have polymorphism in the sequence of this gene, it can be used as a marker. CAPS and size_poly will be described later.

TABLE 8-1 Name Primers used (SEQ ID NO:) marker type bah55e06 5783 5784 size_poly (codominant) bags30b07 5787 5788 SNP baak2l03 5791 5792 CAPS BaAK17D13 5795 5796 SNP basd26l20 5799 5800 SNP bah61p17 5803 5804 SNP BaGS11O06 5807 5808 size_poly (codominant) kr12H0216 5811 5812 SNP BaGS32E23 5815 5816 CAPS basd13k20 5819 5820 CAPS BaAK24O11 5823 5824 CAPS kr26D0507 5827 5828 SNP BaSD2D08 5831 5832 CAPS baak41n21 5835 5836 SNP bah11b15 5839 5840 CAPS kr24B0903 5843 5844 SNP bast50E0709 5847 5848 size_poly (codominant) baal17o01 5851 5852 SNP baak41a04 5855 5856 SNP BaH28C07 5859 5860 size_poly (codominant) bags16g18 5863 5864 SNP BaSD3C22 5867 5868 SNP BaGS17B21 5871 5872 CAPS basd27b10 5875 5876 SNP bah47d23 5879 5880 SNP BaAK21D02 5883 5884 CAPS BaH17D02 5887 5888 CAPS baal4f12 5891 5892 CAPS baGS11I03 5895 5896 CAPS bah56a03 5899 5900 size_poly (codominant) kr16A0501 5903 5904 size_poly (codominant) bah19f01 5907 5908 CAPS BaAK27F07 5911 5912 CAPS bah47f18 5915 5916 size_poly (codominant) bah45l19 5919 5920 size_poly (codominant) BaAK12I12 5923 5924 SNP BaAL6N04 5927 5928 SNP baal16l05 5931 5932 CAPS BaSD18O20 5935 5936 size_poly (codominant) BaSD3J13 5939 5940 SNP bah63j19 5943 5944 size_poly (codominant) BaH36O18 5947 5948 CAPS BaAK20A06 5951 5952 CAPS BaGS8G13 5955 5956 CAPS bah25n06 5959 5960 CAPS BaAK1P06 5963 5964 size_poly (dominant) BaAK16M07 5967 5968 SNP BaH36M15 5971 5972 SNP BaGS12K12 5975 5976 size_poly (dominant) BaAL39C22 5979 5980 SNP kr68B0303 5983 5984 SNP

TABLE 8-2 Name Primers used (SEQ ID NO:) marker type baak24d09 5987 5988 SNP BaGS11J13 5991 5992 CAPS baal38l23 5995 5996 CAPS BaAL36B15 5999 6000 CAPS bast60D0610 6003 6004 CAPS kr22D0808 6007 6008 CAPS baal19i12 6011 6012 SNP basd23o02 6015 6016 CAPS baak1j14 6019 6020 CAPS baak24k18 6023 6024 CAPS kr18G0814 6027 6028 SNP BaH38H09 6031 6032 CAPS BaGS8B13 6035 6036 SNP BaH25J08 6039 6040 SNP baak3d11 6043 6044 SNP baal12p08 6047 6048 SNP bags39i20 6051 6052 SNP bags32m16 6055 6056 size_poly (codominant) kr15A0402 6059 6060 SNP baal3c01 6063 6064 SNP baak41i03 6067 6068 SNP BaGS29M13 6071 6072 SNP basd17m22 6075 6076 CAPS bags15g01 6079 6080 CAPS BaH24I06 6083 6084 SNP BaH57E12 6087 6088 CAPS BaSD25C22 6091 6092 SNP baal19m17 6095 6096 CAPS bah46p14 6099 6100 SNP bags18d19 6103 6104 SNP BaAK34J19 6107 6108 SNP bags6d01 6111 6112 CAPS bags3P11 6115 6116 CAPS BaAK14J21 6119 6120 CAPS baak2m05 6123 6124 SNP baak37g19 6127 6128 CAPS BaGS31B11 6131 6132 SNP BaAL35M08 6135 6136 SNP BaSD16G03 6139 6140 CAPS BaH58J20 6143 6144 SNP BaGS22B13 6147 6148 SNP BaAK43M01 6151 6152 SNP bah14i07 6155 6156 SNP BaH13F14 6159 6160 CAPS baal9i16 6163 6164 CAPS basd27b20 6167 6168 CAPS BaAK2O24 6171 6172 SNP basd18l16 6175 6176 CAPS BaAL19J14 6179 6180 CAPS bags22j15 6183 6164 SNP baak44a23 6187 6188 SNP

TABLE 8-3 Name Primers used (SEQ ID NO:) marker type BaAK32N04 6191 6192 SNP basd21e22 6195 6196 SNP BaGS7K03 6199 6200 size_poly (codominant) baak42e22 6203 6204 SNP bast21D0808 6207 6208 size_poly (codominant) bast75E0610 6211 6212 SNP baet16A1002 6215 6216 SNP basd17m16 6219 6220 CAPS bags17g08 6223 6224 size_poly (codominant) baak21i01 6227 6228 SNP baak13n06 6231 6232 CAPS baak22o23 6235 6236 SNP baet42E0410 6239 6240 size_poly (codominant) BaGS27M04 6243 6244 CAPS BaH52H18 6247 6248 SNP baak33k20 6251 6252 SNP bags21f16 6255 6256 SNP bags34e05 6259 6260 SNP bags15d20 6263 6264 CAPS bags18g10 6267 6268 size_poly (codominant) bags19a02 6271 6272 SNP BaAK17J19 6275 6276 size_poly (codominant) bags35k02 6279 6280 SNP bah35a22 6283 6284 SNP BaGS17H13 6287 6288 SNP bah47n12 6291 6292 SNP baak12p11 6295 6296 SNP baet38B1004 6299 6300 SNP BaGS13K12 6303 6304 SNP BaAL25A05 6307 6308 SNP bah27k23 6311 6312 size_poly (codominant) BaAK24J12 6315 6316 SNP BaSD14M22 6319 6320 SNP bah17l24 6323 6324 SNP baal29j09 6327 6328 SNP bah16d09 6331 6332 SNP bah60d03 6335 6336 SNP bah45i13 6339 6340 SNP bah11h03 6343 6344 SNP bah47h17 6347 6348 SNP bags35d02 6351 6352 SNP bags37e17 6355 6356 CAPS baak14c12 6359 6360 SNP baak34c01 6363 6364 SNP BaH35A11 6367 6368 SNP BaAK30M16 6371 6372 SNP BaSD11P04 6375 6376 CAPS bah12j09 6379 6380 CAPS BaAL2G20 6383 6384 SNP bags14j09 6387 6388 CAPS kr26E0610 6391 6392 CAPS

TABLE 8-4 Name Primers used (SEQ ID NO:) marker type bah37f01 6395 6396 CAPS bags29m05 6399 6400 SNP BaGS17A18 6403 6404 CAPS baet34A0501 6407 6408 SNP BaSD22O13 6411 6412 CAPS bags15a22 6415 6416 CAPS baet21H1016 6419 6420 CAPS BaH30E13 6423 6424 SNP bags4e23 6427 6428 SNP baak45b03 6431 6432 SNP bah12o12 6435 6436 SNP baak34k14 6439 6440 CAPS baal3e14 6443 6444 SNP bags22f12 6447 6448 CAPS BaH45P03 6451 6452 CAPS basd11d13 6455 6456 CAPS Bmag211 — — SSR HVM20 — — SSR basd1j14 6459 6460 SNP bags35b18 6463 6464 SNP baal41i11 6467 6468 SNP bags13e23 6471 6472 SNP bah20d03 6475 6476 SNP baet19F0212 6479 6480 SNP bags12j05 6483 6484 SNP BaSD24D17 6487 6488 SNP BaGS24K10 6491 6492 SNP baal9e05 6495 6496 SNP BaGS33M23 6499 6500 SNP bah46g14 6503 6504 SNP bags33h05 6507 6508 SNP baak24h12 6511 6512 SNP BaH50N19 6515 6516 SNP BaAK31O05 6519 6520 SNP BaGS39L14 6523 6524 SNP BaGS27C22 6527 6528 SNP bags20o24 6531 6532 SNP bags34j05 6535 6536 CAPS bah56l03 6539 6540 CAPS basd12k03 6543 6544 CAPS BaAK39I18 6547 6548 CAPS bags21h06 6551 6552 CAPS bah56k04 6555 6556 CAPS bah60e11 6559 6560 CAPS baak22i05 6563 6564 CAPS baal5i02 6567 6568 CAPS BaAK2E05 6571 6572 CAPS baak33g12 6575 6576 CAPS bags4e05 6579 6580 CAPS bast42C0806 6583 6584 SNP baak26c05 6587 6588 SNP

TABLE 8-5 Name Primers used (SEQ ID NO:) marker type bags19a16 6591 6592 SNP kr11F1212 6595 6596 SNP BaH28K13 6599 6600 CAPS baak20d06 6603 6604 size_poly (codominant) BaSD1I24 6607 6608 CAPS baak35l20 6611 6612 SNP bah57m07 6615 6616 SNP bah60k11 6619 6620 SNP basd20o09 6623 6624 SNP basd24i22 6627 6628 SNP bags30g20 6631 6632 SNP bags1m11 6635 6636 SNP bags23g20 6639 6640 SNP BaGS15B05 6643 6644 SNP BaGS24P05 6647 6648 SNP bags10g06 6651 6652 SNP bah32o04 6655 6656 SNP BaGS32P08 6659 6660 SNP bags4e02 6663 6664 CAPS bast04H0315 6667 6668 CAPS BaGS37L06 6671 6672 CAPS basd2b18 6675 6676 CAPS bags3h12 6679 6680 SNP bags1e21 6683 6684 SNP BaAK27M21 6687 6688 SNP BaSD18F05 6691 6692 size_poly (codominant) BaH35B05 6695 6696 SNP basd21h11 6699 6700 SNP BaH32E20 6703 6704 SNP BaGS22A20 6707 6708 SNP BaSD14B13 6711 6712 CAPS bags29l04 6715 6716 SNP bags22g16 6719 6720 SNP BaAL4B14 6723 6724 size_poly (codominant) bah61h20 6727 6728 SNP bah15p01 6731 6732 SNP baak41p03 6735 6736 SNP BaSD12L06 6739 6740 SNP BaSD23P07 6743 6744 SNP BaGS7J05 6747 6748 SNP bah29b06 6751 6752 SNP BaGS31N17 6755 6756 SNP BaGS13F08 6759 6760 SNP BaAK39G10 6763 6764 CAPS BaAK39G03 6767 6768 CAPS baak2a18 6771 6772 CAPS bags39d12 6775 6776 SNP basd17l04 6779 6780 CAPS BaAK20B19 6783 6784 SNP bags15j15 6787 6788 SNP BaAK30F02 6791 6792 CAPS

TABLE 8-6 Name Primers used (SEQ ID NO:) marker type BaGS22A21 6795 6796 SNP baet45E0410 6799 6800 SNP BaGS22C14 6803 6804 SNP bah23k12 6807 6808 SNP BaH15O13 6811 6812 SNP kr24F0412 6815 6816 SNP BaGS28O21 6819 6820 SNP BaAL15N19 6823 6824 SNP bags10j15 6827 6828 CAPS bags32j03 6831 6832 SNP bags20e14 6835 6836 SNP bags3c15 6839 6840 CAPS basd27n01 6843 6844 SNP BaGS6M19 6847 6848 SNP baak13e03 6851 6852 SNP BaH15M10 6855 6856 CAPS bags14k12 6859 6860 size_poly (codominant) bast61E0509 6863 6864 CAPS BaGS31N04 6867 6868 CAPS BaAK27D22 6871 6872 CAPS baal5o19 6875 6876 CAPS bah55b18 6879 6880 CAPS bah23i02 6883 6884 SNP BaAK15H22 6887 6888 SNP bags32b03 6891 6892 SNP baak30c15 6895 6896 SNP BaGS22A05 6899 6900 SNP bah16m01 6903 6904 SNP bags15e08 6907 6908 CAPS BaGS37D12 6911 6912 SNP BaAL37N24 6915 6916 SNP BaH28B09 6919 6920 SNP bah47h08 6923 6924 SNP BaH57N07 6927 6928 SNP baak38o02 6931 6932 SNP baak20f16 6935 6936 SNP baak28n19 6939 6940 SNP bah59j07 6943 6944 SNP bah44j20 6947 6948 SNP BaH47J05 6951 6952 CAPS baet39B0303 6955 6956 SNP BaAK27D19 6959 6960 SNP basd25g01 6963 6964 SNP BaAL34K17 6967 6968 SNP basd21i17 6971 6972 SNP bags15o12 6975 6976 SNP baal2j10 6979 6980 CAPS baak44n10 6983 6984 SNP baak21e08 6987 6988 SNP BaGS37F14 6991 6992 CAPS BaAK41I21 6995 6996 SNP

TABLE 8-7 Name Primers used (SEQ ID NO:) marker type BaGS19C07 6999 7000 SNP BaGS15O08 7003 7004 SNP baak40p22 7007 7008 SNP BaH21K05 7011 7012 CAPS baal40n03 7015 7016 SNP bags23b08 7019 7020 SNP BaGS39L18 7023 7024 SNP BaGS25K24 7027 7028 CAPS bags1a18 7031 7032 CAPS baal15k07 7035 7036 size_poly (codominant) bags14o13 7039 7040 size_poly (dominant) BaH18D15 7043 7044 SNP bastl04F0911 7047 7048 SNP BaGS31E03 7051 7052 SNP BaGS19J21 7055 7056 SNP BaGS39P08 7059 7060 SNP BaH56B06 7063 7064 SNP baal13m24 7067 7068 SNP bags15h14 7071 7072 SNP bags35j22 7075 7076 CAPS baak20h22 7079 7080 CAPS baal34b14 7083 7084 SNP bah16j04 7087 7088 CAPS BaH54J03 7091 7092 CAPS bast58C1206 7095 7096 SNP BaH26M05 7099 7100 CAPS bastl20B0404 7103 7104 SNP baak14e23 7107 7108 CAPS BaH15P22 7111 7112 SNP BaGS17I22 7115 7116 SNP bags15h01 7119 7120 SNP bags7p13 7123 7124 SNP BaGS29H13 7127 7128 SNP bah47b01 7131 7132 SNP bastl45E1109 7135 7136 CAPS bags32m15 7139 7140 SNP basd12c09 7143 7144 size_poly (codominant) baal27m11 7147 7148 SNP basd12k01 7151 7152 CAPS baak41n15 7155 7156 size_poly (codominant) bags1b01 7159 7160 SNP BaH56O11 7163 7164 size_poly (codominant) bah15k16 7167 7168 CAPS bags31a22 7171 7172 CAPS bags21e21 7175 7176 SNP baak32n05 7179 7180 size_poly (codominant) baet02B0503 7183 7184 SNP BaH29L05 7187 7188 SNP baet43H1016 7191 7192 SNP BaGS38N20 7195 7196 CAPS BaGS23O09 7199 7200 CAPS

TABLE 8-8 Name Primers used (SEQ ID NO:) marker type bastl26E0410 7203 7204 CAPS BaH15N14 7207 7208 CAPS baak20b06 7211 7212 SNP BaH16I04 7215 7216 SNP bah22p07 7219 7220 SNP baak21j02 7223 7224 SNP bah30o13 7227 7228 CAPS bags38fl8 7231 7232 CAPS bah13o05 7235 7236 CAPS baak36b12 7239 7240 CAPS bags18o09 7243 7244 CAPS BaAK16L10 7247 7248 CAPS BaAK38E16 7251 7252 size_poly (dominant) bah13e15 7255 7256 CAPS bags1f22 7259 7260 CAPS bags21o12 7263 7264 SNP BaGS9B14 7267 7268 SNP BaAL1N23 7271 7272 CAPS baaK1a17 7275 7276 SNP BaH32B01 7279 7280 CAPS BaSD18L13 7283 7284 CAPS baak12p07 7287 7288 size_poly (codominant) BaH58A04 7291 7292 CAPS bags1p04 7295 7296 SNP BaAL17O03 7299 7300 SNP baal8e17 7303 7304 SNP BaH50I05 7307 7308 CAPS bastl28A0101 7311 7312 CAPS BaH39L18 7315 7316 SNP bags18e18 7319 7320 SNP WMCIE8 — — SSR

TABLE 9-1 Name Primers used (SEQ ID NO:) marker type BaGS20L10 7323 7324 SNP BaSD15P22 7327 7328 SNP bags7b16 7331 7332 SNP BaAK34H02 7335 7336 CAPS BaAK22H13 7339 7340 SNP BaGS37P19 7343 7344 CAPS BaAL19P17 7347 7348 CAPS BaH41L14 7351 7352 SNP BaAK24H17 7355 7356 SNP bast21A0602 7359 7360 SNP BaAL27L20 7363 7364 SNP Bmac134 — — SSR cMWG682 — — STS bags34p10 7367 7368 SNP basd18b14 7371 7372 size_poly (codominant) bags38p20 7375 7376 CAPS BaAK41N22 7379 7380 CAPS BaAK21D17 7383 7384 CAPS BaAL29B07 7387 7388 SNP baak20o16 7391 7392 SNP bast42A0602 7395 7396 CAPS BaH36B07 7399 7400 SNP BaAK26L07 7403 7404 CAPS baal12a06 7407 7408 CAPS BaSD3C20 7411 7412 SNP bastl17G0113 7415 7416 SNP baak11h14 7419 7420 SNP baal12m14 7423 7424 CAPS baal33a18 7427 7428 SNP bah28a18 7431 7432 SNP bags39o04 7435 7436 SNP BaH48H04 7439 7440 SNP BaGS6B11 7443 7444 size_poly (dominant) bags4p16 7447 7448 SNP basd24j22 7451 7452 SNP basd16p15 7455 7456 SNP bags15k16 7459 7460 SNP bast23D1208 7463 7464 SNP BaAL32B22 7467 7468 SNP BaGS39D07 7471 7472 size_poly (codominant) BaH19L09 7475 7476 SNP bags38a17 7479 7480 SNP BaAK39I11 7483 7484 SNP BaSD3I24 7487 7488 SNP BaH35F01 7491 7492 CAPS BaAL30K02 7495 7496 CAPS bah13l23 7499 7500 SNP BaAL26H21 7503 7504 SNP BaGS22H22 7507 7508 CAPS bags13n11 7511 7512 CAPS bags1h03 7515 7516 CAPS

TABLE 9-2 Name Primers used (SEQ ID NO:) marker type baak32l16 7519 7520 SNP baal19a12 7523 7524 CAPS BaAK45G16 7527 7528 CAPS BaGS37N19 7531 7532 CAPS baak3f03 7535 7536 CAPS bags5c02 7539 7540 SNP BaGS35P07 7543 7544 SNP HVM36 — — SSR bah45e07 7547 7548 size_poly (codominant) BaAK24B09 7551 7552 SNP bags33a11 7555 7556 SNP bah62i11 7559 7560 CAPS BaH56A24 7563 7564 SNP BaSD21D14 7567 7568 SNP basd1a17 7571 7572 SNP bah17n24 7575 7576 CAPS kr70G0113 7579 7580 SNP basd15f08 7583 7584 SNP BaGS4J04 7587 7588 CAPS baak33f06 7591 7592 CAPS basd14f16 7595 7596 CAPS BaH59K20 7599 7600 CAPS baal35h05 7603 7604 SNP BaGS20M01 7607 7608 CAPS BaH25N22 7611 7612 size_poly (codominant) baak14a24 7615 7616 SNP baak30d07 7619 7620 SNP bah11n18 7623 7624 CAPS kr14C0305 7627 7628 SNP baet18F0911 7631 7632 CAPS BaGS4J18 7635 7636 CAPS bast74C0705 7639 7640 SNP BaAL4G17 7643 7644 SNP bastl43H0515 7647 7648 SNP BaH58M22 7651 7652 SNP bags10i21 7655 7656 SNP bah20h16 7659 7660 SNP BaH17P13 7663 7664 SNP baak41d10 7667 7668 CAPS BaGS14F01 7671 7672 CAPS BaAK16E24 7675 7676 SNP BaSD18F09 7679 7680 SNP baet42A0501 7683 7684 SNP BaAK20L07 7687 7688 SNP baak16e20 7691 7692 SNP BaGS13N14 7695 7696 size_poly (codominant) basd13m14 7699 7700 SNP bags20g23 7703 7704 SNP basd1l17 1707 7708 SNP basd26l01 7711 7712 SNP BaAL37J18 7715 7716 SNP

TABLE 9-3 Name Primers used (SEQ ID NO:) marker type bags7i05 7719 7720 SNP bah60m17 7723 7724 SNP bags21l01 7727 7728 SNP baet31E0109 7731 7732 SNP bags34m12 7735 7736 SNP bah50n02 7739 7740 SNP BaH50M11 7743 7744 SNP bast27E1010 7747 7748 SNP bags23c12 7751 7752 SNP bah51m11 7755 7756 SNP baak20d17 7759 7760 SNP bags23l21 7763 7764 SNP BaH52K04 7767 7768 CAPS BaAL37F24 7771 7772 CAPS bags23d01 7775 7776 CAPS BaSD11K22 7779 7780 CAPS baal29m02 7783 7784 SNP bah17e21 7787 7788 SNP bah56j18 7791 7792 SNP bags38n06 7795 7796 CAPS baet44D1208 7799 7800 CAPS BaAL34O13 7803 7804 SNP BaGS4N05 7807 7808 SNP BaSD15P20 7811 7812 SNP baal13d11 7815 7816 CAPS bah27g02 7819 7820 SNP basd27m10 7823 7824 SNP basd23f16 7827 7828 SNP bah16i19 7831 7832 SNP BaH50I20 7835 7836 SNP BaH34M23 7839 7840 CAPS bah28b24 7843 7844 SNP BaH50P13 7847 7848 CAPS basd11m16 7851 7852 CAPS bags10p15 7855 7856 CAPS bags4g01 7859 7860 CAPS bags10k08 7863 7864 CAPS bags5e16 7867 7868 CAPS bags18l02 7871 7872 CAPS baak44k02 7875 7876 CAPS bags35a20 7879 7880 CAPS BaGS26M11 7883 7884 CAPS basd14h21 7887 7888 CAPS BaSD13D12 7891 7892 CAPS bags38k23 7895 7896 size_poly (codominant) BaH42E05 7899 7900 CAPS bastl55A0701 7903 7904 CAPS bags13g18 7907 7908 SNP BaH15D23 7911 7912 SNP kr28B0604 7915 7916 CAPS bags10f01 7919 7920 size_poly (codominant)

TABLE 9-4 Name Primers used (SEQ ID NO:) marker type BaGS33I07 7923 7924 SNP BaSD27B02 7927 7928 SNP BaGS30N12 7931 7932 SNP basd3h13 7935 7936 SNP BaGS37L19 7939 7940 SNP bah16g18 7943 7944 CAPS bags20i15 7947 7948 SNP bags35c23 7951 7952 CAPS bah56k07 7955 7956 SNP bags37d02 7959 7960 SNP BaAK19K05 7963 7964 CAPS bah11i16 7967 7968 CAPS bags22l23 7971 7972 SNP kr33A0901 7975 7976 SNP bastl38C0606 7979 7980 SNP baak11j13 7983 7984 SNP BaH58E19 7987 7988 SNP BaGS31G22 7991 7992 SNP bah37h01 7995 7996 SNP BaAL29P13 7999 8000 SNP bags39a22 8003 8004 SNP bah19g10 8007 8008 SNP BaAL31A14 8011 8012 SNP BaH50G15 8015 8016 SNP BaH51M12 8019 8020 SNP bags18k22 8023 8024 SNP BaGS20N21 8027 8028 SNP BaSD17O21 8031 8032 SNP BaH37G17 8035 8036 SNP bags30l22 8039 8040 SNP baak22b17 8043 8044 CAPS bags20f22 8047 8048 CAPS bast72G0113 8051 8052 SNP BaH30B05 8055 8056 SNP BaH60B14 8059 8060 SNP BaH17B16 8063 8064 SNP basd12n23 8067 8068 SNP BaH61A21 8071 8072 SNP BaAK19H17 8075 8076 SNP bah47l12 8079 8080 SNP baak18p11 8083 8084 SNP baet29H0715 8087 8088 SNP BaH13D11 8091 8092 SNP baet39D1107 8095 8096 SNP BaH53E15 8099 8100 SNP bags32o15 8103 8104 SNP bast48A0701 8107 8108 SNP bast62D0907 8111 8112 SNP BaH45P22 8115 8116 SNP BaGS32L16 8119 8120 SNP BaAK30J05 8123 8124 SNP

TABLE 9-5 Name Primers used (SEQ ID NO:) marker type bah52a14 8127 8128 SNP bah58i22 8131 8132 SNP BaH56L16 8135 8136 SNP bah52a21 8139 8140 SNP BaSD14G11 8143 8144 SNP bags6a03 8147 8148 SNP bast72E0109 8151 8152 SNP bags21a21 8155 8156 SNP BaH58P13 8159 8160 SNP bah27n22 8163 8164 SNP bags20c13 8167 8168 SNP bags30i14 8171 8172 SNP BaAK17E11 8175 8176 SNP baal1d17 8179 8180 CAPS bags22f06 8183 8184 CAPS baal10l01 8187 8188 SNP baak26e17 8191 8192 SNP bah16a03 8195 8196 CAPS BaAL11F18 8199 8200 CAPS BaAK29E10 8203 8204 CAPS bags32d21 8207 8208 SNP bah54j22 8211 8212 SNP baak16f14 8215 8216 CAPS baak44c12 8219 8220 CAPS bah13f11 8223 8224 SNP baal39m19 8227 8228 CAPS baak4e02 8231 8232 CAPS baak46f04 8235 8236 CAPS BaAK13N23 8239 8240 size_poly (codominant) BaGS20E09 8243 8244 size_poly (codominant) baal4f01 8247 8248 CAPS BaSD19I17 8251 8252 CAPS baal4l21 8255 8256 SNP BaAK19P01 8259 8260 CAPS BaAK31O14 8263 8264 SNP BaH26P22 8267 8268 CAPS baal13d17 8271 8272 SNP bags22j12 8275 8276 SNP bags33p05 8279 8280 SNP bags38j07 8283 8284 SNP kr59F0311 8287 8288 CAPS baal13e10 8291 8292 CAPS bags15d19 8295 8296 SNP BaGS5K11 8299 8300 CAPS BaGS10J14 8303 8304 CAPS baak41m17 8307 8308 CAPS bah57o03 8311 8312 SNP baak41d22 8315 8316 CAPS bah61c16 8319 8320 CAPS BaAL7M13 8323 8324 CAPS BaAK31F11 8327 8328 CAPS

TABLE 9-6 Name Primers used (SEQ ID NO:) marker type basd22k05 8331 8332 Size_poly (codominant) basd3p19 8335 8336 Size_poly (dominant) baak17o09 8339 8340 CAPS baal33d23 8343 8344 CAPS BaAK12F04 8347 8348 SNP bah53j16 8351 8352 SNP BaSD2E24 8355 8356 SNP bags39h08 8359 8360 SNP BaSD1N02 8363 8364 SNP bags20m21 8367 8368 SNP baal10h19 8371 8372 SNP basd12m11 8375 8376 SNP bah29d24 8379 8380 SNP BaGS39P09 8383 8384 SNP BaGS31F17 8387 8388 SNP BaGS23D08 8391 8392 SNP bags39d15 8395 8396 SNP BaGS34I17 8399 8400 SNP BaH32N02 8403 8404 SNP BaSD18H19 8407 8408 SNP bast63A0101 8411 8412 SNP BaH50N04 8415 8416 SNP baal4a13 8419 8420 SNP baal41l18 8423 8424 SNP BaH31A03 8427 8428 CAPS bastl39A0901 8431 8432 CAPS baet46D0507 8435 8436 CAPS baal27e20 8439 8440 SNP bags37l16 8443 8444 SNP baak34o06 8447 8448 SNP BaAL30I11 8451 8452 SNP BaH62C15 8455 8456 SNP basd12i15 8459 8460 CAPS BaAK40I03 8463 8464 CAPS BaAK23E14 8467 8468 CAPS BaAL21F11 8471 8472 size_poly (codominant) BaAK16L19 8475 8476 SNP baal27a24 8479 8480 CAPS kr41H0315 8483 8484 SNP BaH62I23 8487 8488 SNP bah52i24 8491 8492 SNP bastl33A0301 8495 8496 SNP BaH28J15 8499 8500 CAPS bastl56A0301 8503 8504 SNP baal39a03 8507 8508 SNP baal5b15 8511 8512 CAPS baak34l03 8515 8516 SNP BaSD20J15 8519 8520 SNP bastl30F0711 8523 8524 SNP baal11p07 8527 8528 size_poly (codominant) BaH28J10 8531 8532 CAPS

TABLE 9-7 Name Primers used (SEQ ID NO:) marker type baak34p05 8535 8536 CAPS bast56G0713 8539 8540 SNP bah27d18 8543 8544 CAPS bastl02H0715 8547 8548 SNP bah37e07 8551 8552 size_poly (codominant) bags4d18 8555 8556 size_poly (codominant) bags8i05 8559 8560 SNP BaH50A15 8563 8564 CAPS kr06D0907 8567 8568 size_poly (codominant) basd18m04 8571 8572 SNP basd2f22 8575 8576 CAPS BaH47K06 8579 8580 CAPS BaH28G01 8583 8584 SNP BaSD1M13 8587 8588 SNP bags37h19 8591 8592 SNP bags29b01 8595 8596 CAPS BaH21A10 8599 8600 SNP bags23d06 8603 8604 SNP basd2a21 8607 8608 CAPS BaGS19G10 8611 8612 SNP cMWG699 — — STS bah11b24 8615 8616 SNP bast39D0408 8619 8620 SNP baak33e08 8623 8624 SNP bags33m15 8627 8628 CAPS BaAL33B08 8631 8632 SNP bags39e12 8635 8636 SNP baak41e24 8639 8640 CAPS bah63c08 8643 8644 CAPS bags33n09 8647 8648 SNP Bmag125 — — SSR baal32h09 8651 8652 SNP BaAK20O17 8655 8656 SNP BaGS32G10 8659 8660 SNP baet33E1210 8663 8664 SNP BaH50O08 8667 8668 SNP bast46F0711 8671 8672 SNP BaH16F05 8675 8676 SNP baal17e18 8679 8680 CAPS cMWG694 — — STS bah13o11 8683 8684 SNP basd11g04 8687 8688 CAPS bah32c06 8691 8692 size_poly (codominant) basd27i13 8695 8696 SNP kr14F0911 8699 8700 size_poly (codominant) BaGS36A04 8703 8704 CAPS bags13a16 8707 8708 CAPS bags26e20 8711 8712 SNP baet45G1214 8715 8716 SNP BaAK35F14 8719 8720 CAPS BaAK23N21 8723 8724 size_poly (codominant)

TABLE 9-8 Name Primers used (SEQ ID NO:) marker type basd13j22 8727 8728 SNP BaAK46E10 8731 8732 SNP bastl55F0812 8735 8736 SNP baak46l06 8739 8740 CAPS BaAL2D11 8743 8744 size_poly (codominant) baak15p17 8747 8748 CAPS BaGS33J16 8751 8752 CAPS bags7p21 8755 8756 CAPS BaAK22E05 8759 8760 CAPS bah42m05 8763 8764 CAPS BaAK25L01 8767 8768 CAPS bags39e24 8771 8772 CAPS BaH56N24 8775 8776 size_poly (codominant) baak44i12 8779 8780 SNP bah21j03 8783 8784 SNP kr71B0103 8787 8788 SNP BaGS16D15 8791 8792 CAPS baak21p23 8795 8796 SNP BaGS6G09 8799 8800 SNP BaSD15M02 8803 8804 SNP basd13f02 8807 8808 SNP BaH19F21 8811 8812 CAPS bags20b10 8815 8816 CAPS bah26j10 8819 8820 SNP bast65G0113 8823 8824 SNP baak4k13 8827 8828 SNP baal19j23 8831 8832 size_poly (codominant) bags34h11 8835 8836 SNP bags37j03 8839 8840 SNP baal15e13 8843 8844 size_poly (codominant) BaAL5O10 8847 8848 size_poly (dominant) BaGS29J10 8851 8852 CAPS bah22o08 8855 8856 SNP bags6k13 8859 8860 SNP bastl43C0705 8863 8864 SNP BaAL4D10 8867 8868 size_poly (codominant) basd12n12 8871 8872 CAPS bags23h03 8875 8876 SNP bags6l02 8879 8880 CAPS bast63B0703 8883 8884 SNP BaSD13E02 8887 8888 SNP BaSD14P15 8891 8892 CAPS bah13a17 8895 8896 SNP BaH50L23 8899 8900 SNP bags19g04 8903 8904 CAPS bast73E0210 8907 8908 SNP BaH50O21 8911 8912 SNP basd21g05 8915 8916 SNP bah33p11 8919 8920 CAPS baal5i19 8923 8924 SNP bah16e04 8927 8928 SNP

TABLE 9-9 Name Primers used (SEQ ID NO:) marker type baak32p24 8931 8932 SNP EBmac415 — — SSR bags38f12 8935 8936 CAPS basd26p18 8939 8940 CAPS baak45h16 8943 8944 CAPS bah28p12 8947 8948 CAPS bah19a10 8951 8952 CAPS baak13d11 8955 8956 SNP bah49p10 8959 8960 SNP baal32n15 8963 8964 SNP bast09C0305 8967 8968 size_poly (codominant) basd16l09 8971 8972 CAPS BaAK22H04 8975 8976 SNP BaGS15J13 8979 8980 SNP kr66G0414 8983 8984 SNP BaSD22C07 8987 8988 SNP kr71C1105 8991 8992 SNP bags34i11 8995 8996 SNP BaH23K17 8999 9000 SNP bast39D0107 9003 9004 SNP BaH44K24 9007 9008 SNP BaGS18N21 9011 9012 CAPS baak32k15 9015 9016 SNP bah61a13 9019 9020 SNP bags35n11 9023 9024 SNP BaAK24I03 9027 9028 SNP baal4h20 9031 9032 size_poly (codominant) BaH28N23 9035 9036 SNP bags10e13 9039 9040 SNP baak27i10 9043 9044 size_poly (codominant) bags19d13 9047 9048 SNP baak19d04 9051 9052 SNP BaAL34O19 9055 9056 SNP baal12l02 9059 9060 CAPS BaAK1P04 9063 9064 SNP baak35m13 9067 9068 SNP bags35a12 9071 9072 CAPS bah41n09 9075 9076 SNP BaGS23I12 9079 9080 SNP BaH16P20 9083 9084 SNP BaAK42L17 9087 9088 CAPS baak36d23 9091 9092 CAPS bast78C1006 9095 9096 CAPS BaH45O16 9099 9100 SNP bah21h09 9103 9104 SNP bah58p22 9107 9108 SNP bags20l19 9111 9112 SNP bah13i10 9115 9116 SNP BaAK36B07 9119 9120 CAPS baak26b05 9123 9124 CAPS baal7c15 9127 9128 SNP

TABLE 9-10 Name Primers used (SEQ ID NO:) marker type bah63f05 9131 9132 SNP bags15l16 9135 9136 SNP BaGS6N10 9139 9140 CAPS bah41e10 9143 9144 SNP BaH54D08 9147 9148 SNP baak18c01 9151 9152 SNP basd21o07 9155 9156 SNP bah41b23 9159 9160 CAPS basd18g15 9163 9164 size_poly (codominant) baak43o03 9167 9168 SNP bastl30D0408 9171 9172 CAPS bah17p16 9175 9176 CAPS baal13f18 9179 9180 SNP bags18i22 9183 9184 SNP bags9b02 9187 9188 SNP bah11e22 9191 9192 SNP bah58h09 9195 9196 SNP basd16e16 9199 9200 CAPS BaAK4C12 9203 9204 SNP bags37a05 9207 9208 SNP bags9p10 9211 9212 CAPS baak24m01 9215 9216 size_poly (codominant) basd27d09 9219 9220 CAPS baak34a14 9223 9224 size_poly (dominant) baak36a20 9227 9228 SNP BaSD17P09 9231 9232 CAPS bags5m04 9235 9236 CAPS BaGS5E06 9239 9240 CAPS MWG2076 — — STS bags15f03 9243 9244 size_poly (dominant) bags18j23 9247 9248 size_poly (dominant) baal13m04 9251 9252 SNP bags9o24 9255 9256 SNP kr49E0610 9259 9260 CAPS bah12h16 9263 9264 SNP baak33n16 9267 9268 SNP BaGS22E05 9271 9272 CAPS bah26n01 9275 9276 SNP BaGS39E07 9279 9280 SNP BaH45O03 9283 9284 CAPS BaH38A09 9287 9288 SNP

TABLE 10-1 Name Primers used (SEQ ID NO:) marker type BaAL15P01 9291 9292 SNP BaAK42D06 9295 9296 size_poly (dominant) bags12k16 9299 9300 size_poly (codominant) BaAL36H19 9303 9304 size_poly (codominant) bags1d06 9307 9308 SNP bast18A0602 9311 9312 SNP basd22i04 9315 9316 CAPS BaGS31M01 9319 9320 CAPS BaH63H24 9323 9324 SNP basd15n13 9327 9328 CAPS MWG848 — — STS BaAK13C16 9331 9332 SNP BaGS32C19 9335 9336 SNP baak36f08 9339 9340 SNP bastl04C0406 9343 9344 CAPS bastl42D1107 9347 9348 SNP BaGS31B20 9351 9352 size_poly (codominant) baak39l17 9355 9356 CAPS BaAL17J24 9359 9360 CAPS bah15p03 9363 9364 CAPS baak20g06 9367 9368 SNP baal22c16 9371 9372 CAPS baak46o05 9375 9376 SNP basd15h22 9379 9380 SNP BaSD19C07 9383 9384 SNP bags22i13 9387 9388 CAPS baak39a14 9391 9392 SNP bah31e12 9395 9396 SNP BaSD15L22 9399 9400 SNP BaGS20D21 9403 9404 CAPS bags39o21 9407 9408 CAPS basd21j11 9411 9412 SNP BaH48C10 9415 9416 SNP BaH54J07 9419 9420 SNP BaSD19H23 9423 9424 CAPS baak35n06 9427 9428 CAPS bags35b22 9431 9432 CAPS BaAK30H06 9435 9436 CAPS BaAL15M07 9439 9440 CAPS BaGS20N02 9443 9444 CAPS BaAL19L12 9447 9448 CAPS bags25b05 9451 9452 CAPS HvLTPPB — — SSR baal1h04 9455 9456 CAPS baet46B0903 9459 9460 SNP BaGS19F16 9463 9464 SNP baak13g18 9467 9468 SNP BaH45N12 9471 9472 CAPS bast74H0216 9475 9476 SNP bah24l06 9479 9480 size_poly (dominant) BaAK45C14 9483 9484 SNP

TABLE 10-2 Name Primers used (SEQ ID NO:) marker type baal5k12 9487 9488 CAPS kr63F0111 9491 9492 SNP baak13h18 9495 9496 SNP baak12j16 9499 9500 CAPS BaAK28J20 9503 9504 CAPS BaH27G14 9507 9509 CAPS BaH49B13 9511 9512 SNP BaGS35A09 9515 9516 CAPS BaGS38L24 9519 9520 size_poly (dominant) BaAK43H20 9523 9524 CAPS bast16A0802 9527 9528 SNP basd18k01 9531 9532 SNP BaH58D17 9535 9536 SNP BaAK30M07 9539 9540 CAPS bags6a04 9543 9544 CAPS kr15H0915 9547 9548 CAPS bags26d01 9551 9552 SNP basd14k04 9555 9556 SNP BaSD24D11 9559 9560 SNP BaH53L10 9563 9564 SNP BaH60D22 9567 9568 size_poly (codominant) BaSD1G06 9571 9572 size_poly (codominant) BaAK21L13 9575 9576 size_poly (codominant) bah57m03 9579 9580 SNP baal30b10 9583 9584 SNP bah62n16 9587 9588 SNP BaGS22F15 9591 9592 SNP BaH50J14 9595 9596 SNP bah19d23 9599 9600 SNP bags38c06 9603 9604 SNP BaGS25N05 9607 9608 SNP bastl29B0503 9611 9612 SNP baak27d01 9615 9616 CAPS baak43n21 9619 9620 SNP BaAL4F05 9623 9624 size_poly (codominant) baal4a06 9627 9628 SNP bast17D1008 9631 9632 SNP BaGS16B17 9635 9636 SNP BaGS27P18 9639 9640 CAPS BaGS4J14 9643 9644 CAPS BaAK16B19 9647 9648 SNP baal40p07 9651 9652 SNP bah49c19 9655 9656 SNP bast58C0406 9659 9660 SNP BaAK35M24 9663 9664 SNP bags19h13 9667 9668 SNP bah57c21 9671 9672 SNP BaH53P15 9675 9676 SNP BaGS20A10 9679 9680 SNP bast02D0808 9683 9684 SNP bags31c04 9687 9688 SNP

TABLE 10-3 Name Primers used (SEQ ID NO:) marker type BaH32J06 9691 9692 CAPS bah60o22 9695 9696 SNP BaH50C16 9699 9700 SNP BaH57K23 9703 9704 CAPS bags21g23 9707 9708 size_poly (codominant) HVM9 — — SSR Bmac67 — — SSR baak43e04 9711 9712 SNP kr17G1113 9715 9716 SNP bags19k19 9719 9720 SNP baak38b13 9723 9724 SNP baak1d12 9727 9728 SNP BaH28M14 9731 9732 SNP BaH48I15 9735 9736 SNP basd27h23 9739 9740 SNP BaAK21A11 9743 9744 SNP bast04B0804 9747 9748 SNP bah45f13 9751 9752 SNP BaAL8J18 9755 9756 SNP BaGS9D01 9759 9760 SNP baal12d12 9763 9764 CAPS baal4i06 9767 9768 SNP bah26i01 9771 9772 SNP BaGS15C17 9775 9776 SNP baak1k08 9779 9780 SNP bags9b03 9783 9784 SNP baet42G1214 9787 9788 SNP bah18d12 9791 9792 SNP BaSD14G02 9795 9796 CAPS bags22b22 9799 9800 SNP bah13f10 9803 9804 size_poly (codominant) baal36g05 9807 9808 SNP bags33j15 9811 9812 SNP BaAL12H04 9815 9816 size_poly (codominant) BaGS16I18 9819 9820 CAPS bast61D0707 9823 9824 SNP bah11k22 9827 9828 CAPS baak32p21 9831 9832 CAPS bah63l21 9835 9836 CAPS BaGS38D03 9839 9840 CAPS BaSD23A04 9843 9844 SNP BaSD14C15 9847 9848 SNP bast63C0105 9851 9852 SNP bast23C1105 9855 9856 SNP bags23b01 9859 9860 CAPS bags29c09 9863 9864 CAPS bags6b06 9867 9868 CAPS BaAK27G06 9871 9872 CAPS BaAK39D07 9875 9876 CAPS BaGS20G21 9879 9880 CAPS bastl22E0810 9883 9884 size_poly (codominant)

TABLE 10-4 Name Primers used (SEQ ID NO:) marker type bah11m03 9887 9888 CAPS BaAL3C04 9891 9892 CAPS BaAL19H10 9895 9896 SNP bags24n16 9899 9900 CAPS BaSD24B15 9903 9904 SNP bah48n17 9907 9908 SNP bags9i05 9911 9912 SNP bags1l22 9915 9916 SNP bah62p18 9919 9920 SNP bah55n21 9923 9924 SNP bah44a05 9927 9928 SNP bah19c13 9931 9932 SNP baal12b04 9935 9936 CAPS Bmag136 — — SSR Bmac209 — — SSR BaAK28A10 9939 9940 size_poly (dominant) kr28B0703 9943 9944 SNP bastl50C0606 9947 9948 SNP BaGS36B01 9951 9952 CAPS BaH19A05 9955 9956 SNP baal10c06 9959 9960 SNP bags19p04 9963 9964 SNP BaAK21A17 9967 9968 SNP bah20j14 9971 9972 SNP BaAK19A03 9975 9976 SNP BaGS30E19 9979 9980 SNP BaAK28C21 9983 9984 size_poly (codominant) bags11o14 9987 9988 SNP BaGS13P22 9991 9992 SNP kr27A1101 9995 9996 SNP HVM27 — — SSR baal12i18 9999 10000 CAPS BaAL8G07 10003 10004 CAPS bast58F0412 10007 10008 SNP bags3f23 10011 10012 CAPS baal4m06 10015 10016 CAPS baal32p23 10019 10020 SNP basd23m17 10023 10024 SNP bastl30E0509 10027 10028 SNP bah44b0B 10031 10032 SNP BaGS14N10 10035 10036 SNP BaGS32B13 10039 10040 SNP BaH30B03 10043 10044 SNP basd1l23 10047 10048 CAPS basd11o06 10051 10052 SNP bah17f24 10055 10056 SNP BaAK26L17 10059 10060 SNP bags23f03 10063 10064 SNP bags20o06 10067 10068 SNP bags19n12 10071 10072 SNP baal12a09 10075 10076 size_poly (codominant)

TABLE 10-5 Name Primers used (SEQ ID NO:) marker type bast14F0612 10079 10080 SNP baak13p20 10083 10084 CAPS BaGS16K13 10087 10088 SNP baak22c16 10091 10092 SNP baal40m06 10095 10096 SNP BaH29I03 10099 10100 CAPS bah16h01 10103 10104 CAPS bah57a11 10107 10108 SNP BaH41E23 10111 10112 SNP baal11c11 10115 10116 size_poly (dominant) BaSD14L18 10119 10120 SNP bah14d17 10123 10124 SNP BaGS1N17 10127 10128 SNP bags27h17 10131 10132 CAPS bah47p22 10135 10136 SNP BaH50A16 10139 10140 SNP bags7b20 10143 10144 SNP bags22a02 10147 10148 SNP HvBRI1 — — dCAPS basd12g02 10151 10152 SNP basd15o18 10155 10156 SNP baal25d19 10159 10160 SNP bast52G0414 10163 10164 SNP baal11c20 10167 10168 CAPS bags37k06 10171 10172 SNP bags6e22 10175 10176 SNP basd15a02 10179 10180 CAPS bags7b06 10183 10184 CAPS bah15k11 10187 10188 CAPS bast46C0406 10191 10192 SNP bastl45E0509 10195 10196 SNP baal35p14 10199 10200 CAPS BaH41G07 10203 10204 SNP bast46H1016 10207 10208 SNP bah60d12 10211 10212 SNP baal39a19 10215 10216 SNP baak11n24 10219 10220 CAPS bast14E0909 10223 10224 SNP baet13G0713 10227 10228 SNP HVM33 — — SSR BaSD14L04 10231 10232 CAPS baak13c05 10235 10236 SNP bastl41G0513 10239 10240 SNP BaGS39M09 10243 10244 CAPS baal19b12 10247 10248 SNP BaAL39B05 10251 10252 SNP BaAL1J03 10255 10256 SNP basd11k09 10259 10260 SNP BaGS33N15 10263 10264 SNP baet43H0416 10267 10268 SNP bah11m06 10271 10272 SNP

TABLE 10-6 Name Primers used (SEQ ID NO:) marker type baak31a24 10275 10276 CAPS bags27h20 10279 10280 SNP basd27c09 10283 10284 SNP BaAL25O01 10287 10288 SNP bags7f08 10291 10292 CAPS BaGS13O12 10295 10296 size_poly (codominant) bags38b06 10299 10300 CAPS BaAK19J09 10303 10304 SNP bags9e16 10307 10308 size_poly (codominant) bags20n14 10311 10312 SNP bah42o12 10315 10316 SNP baak31k16 10319 10320 SNP bags4p14 10323 10324 SNP bah27a22 10327 10328 CAPS bags21n02 10331 10332 size_poly (codominant) BaH62B09 10335 10336 SNP BaGS19J10 10339 10340 SNP bags15k18 10343 10344 SNP BaAK29G03 10347 10348 CAPS baak23e11 10351 10352 CAPS bags31k04 10355 10356 CAPS bast56C0305 10359 10360 size_poly (dominant) basd26c09 10363 10364 SNP BaSD20B11 10367 10368 SNP HVM60 — — SSR BaH46F11 10371 10372 SNP BaH30F03 10375 10376 SNP BaSD27G02 10379 10380 CAPS Bmag225 — — SSR BaAK33I12 10383 10384 SNP baak32m10 10387 10388 CAPS BaAL4L02 10391 10392 CAPS kr44F0911 10395 10396 size_poly (codominant) BaGS37A16 10399 10400 SNP bags39p06 10403 10404 SNP BaAL12N06 10407 10408 SNP bags22p05 10411 10412 size_poly (codominant) bags9a03 10415 10416 SNP baet24F1212 10419 10420 SNP baak29d10 10423 10424 SNP bags13i12 10427 10428 SNP BaAL16A23 10431 10432 SNP bastl16B1204 10435 10436 SNP bastl29F0711 10439 10440 SNP BaGS32M17 10443 10444 CAPS baal15e10 10447 10448 SNP basd3g08 10451 10452 CAPS baal33m18 10455 10456 SNP baak24e23 10459 10460 SNP baak46j13 10463 10464 SNP bah49o05 10467 10468 SNP

TABLE 10-7 Name Primers used (SEQ ID NO:) marker type baal15e05 10471 10472 SNP BaAK1O08 10475 10476 SNP BaGS30P02 10479 10480 SNP BaSD15D05 10483 10484 SNP BaAL7B16 10487 10488 CAPS bags38n23 10491 10492 SNP baak36a14 10495 10496 SNP baak34g01 10499 10500 CAPS baak12f01 10503 10504 CAPS BaGS30I18 10507 10508 CAPS bags35n24 10511 10512 SNP bah37j21 10515 10516 SNP bah31e13 10519 10520 SNP bags22g10 10523 10524 size_poly (dominant) bags22f17 10527 10528 size_poly (codominant) baal30l05 10531 10532 SNP bags38o10 10535 10536 SNP baak40o04 10539 10540 CAPS BaH49L21 10543 10544 SNP bastl30A0701 10547 10548 SNP bags19l03 10551 10552 SNP bah18m13 10555 10556 SNP baak44p03 10559 10560 SNP kr10H0216 10563 10564 SNP bags11i04 10567 10568 CAPS bah52o06 10571 10572 CAPS bah35c14 10575 10576 size_poly (codominant) BaAL30C02 10579 10580 size_poly (dominant) bah11i21 10583 10584 SNP BaAK36B11 10587 10588 size_poly (codominant) BaAK20K23 10591 10592 SNP basd14n22 10595 10596 SNP BaAL25p17 10599 10600 CAPS BaH49A01 10603 10604 size_poly (codominant) basd13p12 10607 10608 size_poly (codominant) bags39m17 10611 10612 size_poly (codominant) baet44D0707 10615 10616 SNP BaH16P10 10619 10620 SNP BaAK42J01 10623 10624 SNP kr24E0709 10627 10628 CAPS BaAK38O08 10631 10632 SNP BaAL13N01 10635 10636 CAPS baak12c12 10639 10640 size_poly (codominant) BaGS31N06 10643 10644 CAPS baal15f24 10647 10648 CAPS BaSD18B21 10651 10652 SNP BaSD17G23 10655 10656 SNP baak41k22 10659 10660 SNP baak21o03 10663 10664 SNP BaSD16B18 10667 10668 SNP bast79C1105 10671 10672 SNP

TABLE 10-8 Name Primers used (SEQ ID NO:) marker type BaAK4J17 10675 10676 SNP BaAL37H08 10679 10680 SNP BaH36L17 10683 10684 CAPS BaH46A12 10687 10688 size_poly (codominant) bags11i15 10691 10692 SNP baak20g24 10695 10696 CAPS baak20h23 10699 10700 size_poly (codominant) baak35b18 10703 10704 SNP Bmag13 — — SSR baak41o03 10707 10708 SNP baet46C0206 10711 10712 CAPS bags10i20 10715 10716 size_poly (dominant) baal1b16 10719 10720 SNP baal5i05 10723 10724 CAPS BaH27B05 10727 10728 size_poly (codominant) bah14a11 10731 10732 CAPS basd19k10 10735 10736 size_poly (codominant) bags30n17 10739 10740 CAPS bags31e24 10743 10744 CAPS BaGS5B16 10747 10748 SNP BaAL5F06 10751 10752 SNP bags34a11 10755 10756 CAPS bags6c16 10759 10760 CAPS bah42g19 10763 10764 SNP BaH47O14 10767 10768 SNP BaH12L06 10771 10772 SNP basd11a10 10775 10776 CAPS BaH50I12 10779 10780 CAPS BaH51A21 10783 10784 SNP baak45p02 10787 10788 CAPS BaSD12P12 10791 10792 SNP bags9l16 10795 10796 CAPS BaSD26O20 10799 10800 CAPS bags5d10 10803 10804 CAPS baal0e07 10807 10808 CAPS baak14e02 10811 10812 CAPS BaH63F14 10815 10816 size_poly (codominant) bah33f19 10819 10820 CAPS bags19l10 10823 10824 SNP BaH56J21 10827 10828 SNP baal40i22 10831 10832 CAPS kr42C0105 10835 10836 size_poly (codominant) bags16i19 10839 10840 SNP BaH54H01 10843 10844 SNP kr69E0810 10847 10848 CAPS bags17i14 10851 10852 CAPS bags20e19 10855 10856 SNP bast74C0206 10859 10860 SNP BaAK13L10 10863 10864 SNP bags20i01 10867 10868 SNP basd19n14 10871 10872 SNP

TABLE 10-9 Name Primers used (SEQ ID NO:) marker type baak42g21 10875 10876 SNP BaAK39A15 10879 10880 CAPS basd18o21 10883 10884 CAPS BaAK22K17 10887 10888 SNP baal6o24 10891 10892 SNP BaH22C09 10895 10896 SNP HVM62 — — SSR bags23k14 10899 10900 CAPS baal24n12 10903 10904 SNP BaGS21H17 10907 10908 CAPS BaSD16D09 10911 10912 SNP BaAL13B22 10915 10916 SNP bah41l03 10919 10920 CAPS bastl30F0111 10923 10924 SNP BaH15L04 10927 10928 SNP baak40c12 10931 10932 SNP kr23D0408 10935 10936 CAPS bah57d15 10939 10940 SNP baak31e03 10943 10944 SNP BaAL13O01 10947 10948 SNP BaSD26P04 10951 10952 SNP bags20f18 10955 10956 SNP BaH42J22 10959 10960 SNP BaAL3K03 10963 10964 size_poly (codominant) kr30B1103 10967 10968 SNP baak14i02 10971 10972 CAPS bags28c17 10975 10976 CAPS baal4e21 10979 10980 SNP BaGS29D05 10983 10984 SNP bah54d24 10987 10988 SNP basd22g20 10991 10992 CAPS BaH62H20 10995 10996 CAPS bags38h17 10999 11000 SNP BaAK30B23 11003 11004 SNP BaAK24P09 11007 11008 SNP baak23i12 11011 11012 CAPS BaGS22D06 11015 11016 SNP BaAL34P18 11019 11020 SNP BaAL39F24 11023 11024 CAPS BaGS4L04 11027 11028 CAPS bah12e02 11031 11032 CAPS BaAL15F23 11035 11036 SNP BaGS25O15 11039 11040 SNP BaGS7G14 11043 11044 CAPS basd24i11 11047 11048 CAPS baak20l21 11051 11052 size_poly (dominant) bags33m02 11055 11056 SNP BaH48G21 11059 11060 SNP bags27p13 11063 11064 SNP

TABLE 11-1 Name Primers used (SEQ ID NO:) marker type BaGS10N17 11067 11068 size_poly (dominant) BaAK38K23 11071 11072 size_poly (codominant) bah14a22 11075 11076 SNP BaAL19O06 11079 11080 CAPS BaGS32P17 11083 11084 SNP bah11j04 11087 11088 CAPS baak35b06 11091 11092 SNP basd21f17 11095 11096 SNP BaAK44O11 11099 11100 size_poly (dominant) bastl14D0408 11103 11104 size_poly (codominant) BaAK42C15 11107 11108 CAPS baak40n12 11111 11112 CAPS basd2j05 11115 11116 CAPS baak46n05 11119 11120 size_poly (codominant) baak24g04 11123 11124 CAPS BaAL22H02 11127 11128 SNP bah56g24 11131 11132 SNP BaAL2I22 11135 11136 CAPS bags20c22 11139 11140 CAPS BaH50O22 11143 11144 CAPS baak32f06 11147 11148 SNP bags35i24 11151 11152 CAPS BaSD21D13 11155 11156 SNP bast46D0408 11159 11160 SNP bast55B1204 11163 11164 SNP BaGS39M07 11167 11168 size_poly (dominant) bags39b15 11171 11172 SNP baak37p11 11175 11176 CAPS bah29o22 11179 11180 SNP baak12k14 11183 11184 size_poly (codominant) baet31E1010 11187 11188 SNP HVM40 — — SSR baal39h14 11191 11192 SNP bags14g22 11195 11196 size_poly (codominant) baal18m18 11199 11200 SNP BaSD24E02 11203 11204 SNP BaH50N14 11207 11208 CAPS kr70A0202 11211 11212 CAPS baak11d04 11215 11216 SNP MWG2033 — — STS bags20h01 11219 11220 CAPS BaH39P15 11223 11224 CAPS BaAK46O20 11227 11228 CAPS baal33m06 11231 11232 SNP bastl50E0309 11235 11236 SNP bags20i17 11239 11240 SNP BaGS22A07 11243 11244 SNP bah41b09 11247 11248 SNP kr18G0913 11251 11252 CAPS BaGS19O23 11255 11256 SNP bags15e12 11259 11260 CAPS

TABLE 11-2 Name Primers used (SEQ ID NO:) marker type bags29i09 11263 11264 CAPS baak19m23 11267 11268 CAPS bags14h12 11271 11272 SNP bah32e15 11275 11276 SNP bah11l02 11279 11280 CAPS bags39e16 11283 11284 SNP bags10l12 11287 11288 CAPS BaGS31M13 11291 11292 CAPS baal32m10 11295 11296 SNP basd12b23 11299 11300 SNP baal39e15 11303 11304 SNP baal16l11 11307 11308 CAPS BaGS31B01 11311 11312 SNP BaSD17F09 11315 11316 CAPS BaH48L11 11319 11320 SNP BaSD14M08 11323 11324 CAPS bah56c09 11327 11328 SNP baak17g07 11331 11332 size_poly (codominant) bags20k09 11335 11336 SNP BaSD25B08 11339 11340 SNP bast27E0309 11343 11344 SNP baet20D0107 11347 11348 SNP BaAL17L08 11351 11352 CAPS bah61p18 11355 11356 CAPS bah63d12 11359 11360 CAPS BaAK26A03 11363 11364 CAPS baak26n10 11367 11368 SNP bast22B0204 11371 11372 SNP BaAL3C05 11375 11376 SNP BaGS38H14 11379 11380 size_poly (codominant) BaAL4D19 11383 11384 CAPS BaAK20B09 11387 11388 SNP BaAK37H01 11391 11392 SNP BaGS33P13 11395 11396 SNP basd13l12 11399 11400 CAPS bags11m01 11403 11404 CAPS BaGS18H09 11407 11408 SNP basd3d13 11411 11412 CAPS baal9m23 11415 11416 CAPS bags23f08 11419 11420 CAPS BaGS15L23 11423 11424 CAPS baak2k13 11427 11428 CAPS baak17d18 11431 11432 SNP bags13a12 11435 11436 SNP baak44g22 11439 11440 CAPS kr34F0212 11443 11444 SNP baal16d11 11447 11448 SNP bah22d04 11451 11452 SNP bah48m23 11455 11456 size_poly (codominant) basd15p13 11459 11460 CAPS BaGS6O07 11463 11464 SNP

TABLE 11-3 Name Primers used (SEQ ID NO:) marker type BaH37O10 11467 11468 SNP bags21k10 11471 11472 CAPS bah56n18 11475 11476 CAPS baal9i11 11479 11480 SNP BaAL39N06 11483 11484 SNP bags9d05 11487 11488 size_poly (codominant) BaAL19I19 11491 11492 SNP basd12l11 11495 11496 SNP bastl46D1008 11499 11500 SNP BaH50H16 11503 11504 SNP bags9n05 11507 11508 SNP kr61B1103 11511 11512 SNP kr27B0103 11515 11516 SNP kr33H1115 11519 11520 SNP baak30l02 11523 11524 SNP bah55a12 11527 11528 SNP BaH26K14 11531 11532 SNP HVM3 — — SSR BaAK33K19 11535 11536 CAPS basd18m17 11539 11540 SNP BaH50G09 11543 11544 CAPS BaH53B03 11547 11548 SNP BaH54L11 11551 11552 CAPS basd13i14 11555 11556 CAPS bah56g09 11559 11560 CAPS bah41b06 11563 11564 CAPS bags14d19 11567 11568 SNP baal20f05 11571 11572 SNP BaH18H12 11575 11576 SNP baal9o21 11579 11580 SNP bags21a02 11583 11584 SNP BaAL3G19 11587 11588 SNP baal7a09 11591 11592 SNP BaAL29I16 11595 11596 size_poly (codominant) bast70B0804 11599 11600 SNP bags14n08 11603 11604 CAPS bastl14F0412 11607 11608 SNP BaAK36I12 11611 11612 CAPS baal3f19 11615 11616 SNP baal1e10 11619 11620 SNP bags6k02 11623 11624 SNP kr42H0315 11627 11628 SNP bah39p02 11631 11632 SNP BaGS9L14 11635 11636 SNP baal35l03 11639 11640 SNP basd12m15 11643 11644 SNP BaAK35P01 11647 11648 SNP basd11d18 11651 11652 SNP BaH38E07 11655 11656 SNP BaGS18H13 11659 11660 SNP bags18g16 11663 11664 SNP

TABLE 11-4 Name Primers used (SEQ ID NO:) marker type bags27f21 11667 11668 SNP BaGS7M13 11671 11672 CAPS baal39c19 11675 11676 SNP baak46j05 11679 11680 SNP BaAK32D20 11683 11684 CAPS MWG058 — — STS BaGS18M02 11687 11688 SNP bags9c05 11691 11692 size_poly (dominant) baak46m13 11695 11696 CAPS bags35n16 11699 11700 CAPS bags39g08 11703 11704 SNP baal33a06 11707 11708 CAPS BaSD17F20 11711 11712 CAPS bags22i21 11715 11716 CAPS bah32g18 11719 11720 SNP BaAL37O23 11723 11724 CAPS bags22p03 11727 11728 CAPS bags38l18 11731 11732 SNP BaGS35C13 11735 11736 SNP bah47h04 11739 11740 SNP bags30m11 11743 11744 SNP BaH22L15 11747 11748 SNP bags39e15 11751 11752 SNP kr65H0816 11755 11756 CAPS basd11h11 11759 11760 SNP baak2b06 11763 11764 SNP Bmag353 — — SSR bags37j11 11767 11768 CAPS BaAK46L15 11771 11772 CAPS BaSD14A23 11775 11776 SNP bast25C0705 11779 11780 SNP bast21B1204 11783 11784 SNP bags1l16 11787 11788 CAPS BaAK21G02 11791 11792 SNP bastl26E1109 11795 11796 SNP basd13k24 11799 11800 SNP BaAL13F02 11803 11804 SNP bags13c10 11807 11808 SNP bags11o11 11811 11812 SNP BaAK36P01 11815 11816 SNP bah52d09 11819 11820 SNP BaGS26D18 11823 11824 SNP baak33c22 11827 11828 SNP basd1d10 11831 11832 CAPS baak11c22 11835 11836 CAPS bags34l06 11839 11840 SNP baak33j06 11843 11844 CAPS bags23a11 11847 11848 CAPS bags34f02 11851 11852 CAPS bags20p21 11855 11856 SNP bah52m01 11859 11860 SNP

TABLE 11-5 Primers used Name (SEQ ID NO:) marker type basd15d07 11863 11864 SNP BaAL20A14 11867 11868 CAPS BaGS21B04 11871 11872 CAPS HVM68 — — SSR BaAL30B07 11875 11876 SNP basd11p10 11879 11880 CAPS BaGS33E17 11883 11884 CAPS kr67C0206 11887 11888 SNP bags15j20 11891 11892 SNP BaH15E05 11895 11896 SNP BaH58K02 11899 11900 SNP basd13d17 11903 11904 SNP BaGS17A15 11907 11908 CAPS BaAK30F13 11911 11912 SNP baal17m22 11915 11916 CAPS baak42f04 11919 11920 CAPS baal2n22 11923 11924 SNP kr30C0705 11927 11928 SNP BaSD19J21 11931 11932 SNP BaH34N22 11935 11936 SNP BaAK2I20 11939 11940 CAPS BaGS1E22 11943 11944 SNP baak34b17 11947 11948 SNP BaSD11L18 11951 11952 CAPS kr32A0202 11955 11956 SNP bags27h21 11959 11960 SNP bah62d17 11963 11964 SNP bah43e22 11967 11968 SNP BaAL30I23 11971 11972 SNP BaSD13H20 11975 11976 CAPS BaSD14O04 11979 11980 CAPS baak34p06 11983 11984 SNP bah63b08 11987 11988 SNP bastl03F0812 11991 11992 SNP basd14m17 11995 11996 SNP BaSD2J03 11999 12000 SNP bah13b17 12003 12004 size_poly (codominant) baal32b23 12007 12008 SNP bastl50D1208 12011 12012 size_poly (codominant) BaGS9H13 12015 12016 CAPS baal33e04 12019 12020 CAPS BaAL40L16 12023 12024 CAPS bah44n03 12027 12028 SNP bags20h05 12031 12032 SNP bags20l15 12035 12036 SNP BaGS7E03 12039 12040 SNP bags9k13 12043 12044 CAPS bast55E0709 12047 12048 SNP bags8a14 12051 12052 CAPS baak38k04 12055 12056 SNP BaH33B15 12059 12060 CAPS

TABLE 11-6 Primers used Name (SEQ ID ND:) marker type bags29m17 12063 12064 size_poly (codominant) BaSD23P08 12067 12068 CAPS BaH42L12 12071 12072 SNP bast79G0313 12075 12076 CAPS baet46C0905 12079 12080 SNP baal4o09 12083 12084 CAPS bah26e10 12087 12088 SNP BaH32J04 12091 12092 SNP bags20l07 12095 12096 SNP kr18C0505 12099 12100 SNP basd11m24 12103 12104 SNP basd1i15 12107 12108 SNP bah45b02 12111 12112 size_poly (codominant) bast40C0206 12115 12116 CAPS baal40g05 12119 12120 CAPS bah13i21 12123 12124 SNP basd27o20 12127 12128 SNP BaAK12L24 12131 12132 CAPS BaAL12F24 12135 12136 CAPS baak45l08 12139 12140 SNP BaAL36N04 12143 12144 SNP baak41d17 12147 12148 SNP kr39E0810 12151 12152 CAPS BaGS32G16 12155 12156 CAPS BaGS25M06 12159 12160 SNP BaH36F21 12163 12164 SNP BaAK13B12 12167 12168 SNP baal29j18 12171 12172 size_poly (codominant) bast63B0604 12175 12176 SNP baak11n06 12179 12180 SNP kr13F1012 12183 12184 SNP bags20k06 12187 12188 SNP baak15p20 12191 12192 SNP bah18n11 12195 12196 size_poly (codominant) BaH23J08 12199 12200 SNP baet30B1004 12203 12204 SNP bags34p06 12207 12208 size_poly (codominant) bastl33H0816 12211 12212 SNP BaSD17I17 12215 12216 size_poly (codominant) basd19p22 12219 12220 CAPS bags4e03 12223 12224 CAPS BaAK36A13 12227 12228 CAPS bags33i03 12231 22232 CAPS BaAK14F03 12235 12236 CAPS BaAK42K19 12239 12240 SNP bast60A1101 12243 12244 SNP bah52l06 12247 12248 SNP bah23h10 12251 12252 SNP BaGS15O23 12255 12256 SNP BaAK20E08 12259 12260 SNP kr61A1202 12263 12264 SNP

TABLE 11-7 Primers used Name (SEQ ID NO:) marker type bast03F0212 12267 12268 SNP bags39m22 12271 12272 CAPS BaAK30O17 12275 12276 SNP baak29a01 12279 12280 CAPS basd1e04 12283 12284 CAPS BaGS23K09 12287 12288 CAPS BaAL2N04 12291 12292 SNP BaH42C12 12295 12296 CAPS bah39o14 12299 12300 size_poly (codominant) bast26A1202 12303 12304 size_poly (codominant) BaGS30N15 12307 12308 SNP BaAL34D18 12311 12312 SNP baak15k23 12315 12316 CAPS bags37i06 12319 12320 SNP BaGS19N09 12323 12324 SNP BaSD13H09 12327 12328 SNP bags3h19 12331 12332 CAPS baak28o08 12335 12336 SNP BaH15K08 12339 12340 SNP HVM67 — — SSR bah17h20 12343 12344 CAPS BaAL5L13 12347 12348 CAPS BaGS31P13 12351 12352 size_poly (dominant) BaH28A11 12355 12356 SNP BaSD25E01 12359 12360 SNP sh — — Trait baet23F0111 12363 12364 SNP VRN2 — — size_poly bastl41C0806 12367 12368 CAPS basd11o04 12371 12372 SNP bags22kl7 12375 12376 SNP bastl27A1101 12379 12380 size_poly (dominant) bags22a16 12383 12384 CAPS BaAL5E04 12387 12388 CAPS baal39f20 12391 12392 CAPS baak43d13 12395 12396 CAPS baak41l23 12399 12400 CAPS baet23B0604 12403 12404 CAPS BaAK28I08 12407 12408 CAPS bastl26C1206 12411 12412 SNP bah27p20 12415 12416 SNP baal12d24 12419 12420 SNP bastl41F0511 12423 12424 SNP BaGS17E03 12427 12428 CAPS

TABLE 12-1 Primers used Name (SEQ ID NO:) marker type baal10j04 12431 12432 SNP BaAK33J17 12435 12436 SNP BaAL21J19 12439 12440 CAPS bast17H0515 12443 12444 SNP bags22c02 12447 12448 size_poly (codominant) BaAK35A06 12451 12452 SNP BaGS11K08 12455 12456 CAPS bastl30A0602 12459 12460 size_poly (codominant) BaGS39H16 12463 12464 CAPS MWG502 — — STS BaAK18M22 12467 12468 SNP bast26G0614 12471 12472 SNP BaSD22F13 12475 12476 SNP BaAK28L16 12479 12480 CAPS bags35o06 12483 12484 CAPS bags13d07 12487 12488 CAPS BaAL24F18 12491 12492 size_poly (codominant) bah63l18 12495 12496 SNP BaGS9H22 12499 12500 SNP BaAK18A05 12503 12504 CAPS BaH38D03 12507 12508 CAPS BaH17N17 12511 12512 SNP bah18d08 12515 12516 SNP bags34a05 12519 12520 SNP bags1m23 12523 12524 SNP BaH50B05 12527 12528 SNP bags1h11 12531 12532 SNP BaH47G19 12535 12536 CAPS BaAK38H10 12539 12540 CAPS bah47e01 12543 12544 SNP bast52E0109 12547 12548 SNP bags1o08 12551 12552 SNP BaH31H16 12555 12556 CAPS baaK32l14 12559 12560 SNP BaH50O06 12563 12564 SNP BaSD26I01 12567 12568 SNP bags1h24 12571 12572 SNP basd27g16 12575 12576 SNP BaGS16G24 12579 12580 CAPS BaGS34C19 12583 12584 SNP bah20k17 12587 12588 SNP bah54e13 12591 12592 size_poly (codominant) bags35i06 12595 12596 CAPS bah29g09 12599 12600 size_poly (codominant) BaAK29C12 12603 12604 SNP bah56c06 12607 12608 CAPS baal6a09 12611 12612 SNP bags6j06 12615 12616 CAPS BaH30P15 12619 12620 SNP baak30k04 12623 12624 CAPS basd25d22 12627 12628 SNP

TABLE 12-2 Primers used Name (SEQ ID NO:) marker type BaGS39M02 12631 12632 CAPS baak13n10 12635 12636 SNP BaGS18J21 12639 12640 SNP BaSD22G22 12643 12644 size_poly (codominant) bags18b17 12647 12648 size_poly (codominant) bags33j02 12651 12652 SNP baak36b16 12655 12656 CAPS bags37f05 12659 12660 SNP BaAL17L15 12663 12664 SNP BaGS20H12 12667 12668 SNP kr65A0802 12671 12672 SNP BaGS13E12 12675 12676 size_poly (dominant) basd3h06 12679 12680 size_poly (codominant) basd22j16 12683 12684 SNP bah39n18 12687 12688 SNP bags20d19 12691 12692 SNP BaGS34H19 12695 12696 SNP baak42n10 12699 12700 SNP BaSD24F03 12703 12704 SNP BaGS23N21 12707 12708 SNP BaAK45E04 12711 12712 SNP bags5n23 12715 12716 SNP bast52H1216 12719 12720 SNP bags22f23 12723 12724 SNP BaH38E02 12727 12728 SNP BaH50O07 12731 12732 SNP kr07C1006 12735 12736 SNP baak26a02 12739 12740 SNP BaAK2J22 12743 12744 SNP bah45h23 12747 12748 SNP bah11h09 12751 12752 size_poly (dominant) BaAK46M16 12755 12756 SNP bah55m23 12759 12760 SNP bah59c05 12763 12764 SNP basd11l17 12767 12768 SNP bah19e08 12771 12772 SNP BaAK21N24 12775 12776 SNP BaH50E09 12779 12780 SNP BaAK17P18 12783 12784 SNP BaAL7A04 12787 12788 SNP bast47B0303 12791 12792 SNP bah28o17 12795 12796 SNP bah37k03 12799 12800 SNP bastl38A0501 12803 12804 SNP bags23c03 12807 12808 SNP basd13j01 12811 12812 SNP bags21c02 12815 12816 SNP bah28l03 12819 12820 CAPS BaAK36B17 12823 12824 CAPS bags6k10 12827 12828 SNP BaAK32J23 12831 12832 size_poly (codominant)

TABLE 12-3 Primers used Name (SEQ ID NO:) marker type bags9k18 12835 12836 SNP baal18c19 12839 12840 CAPS baak27p14 12843 12844 SNP BaGS30N21 12847 12848 SNP bah55p23 12851 12852 SNP bah54p22 12855 12856 SNP bags34f05 12859 12860 SNP Bmac113 — — SSR bags15i11 12863 12864 CAPS BaH26I21 12867 12868 size_poly (codominant) BaSD20M22 12871 12872 CAPS bah14a24 12875 12876 SNP BaH50J11 12879 12880 SNP bags7a20 12883 12884 CAPS bah25l12 12887 12888 CAPS bags6k09 12891 12892 CAPS BaGS29P21 12895 12896 CAPS bah58f18 12899 12900 CAPS bags39i18 12903 12904 CAPS basd22l21 12907 12908 SNP bags4o22 12911 12912 CAPS kr27E0909 12915 12916 SNP bastl41H0216 12919 12920 SNP baak36o17 12923 12924 SNP BaH31P15 12927 12928 SNP kr07D0208 12931 12932 CAPS bags9h03 12935 12936 SNP BaGS38M11 12939 12940 CAPS kr26C0705 12943 12944 size_poly (dominant) bags3k24 12947 12948 CAPS bah53e16 12951 12952 CAPS bags21n10 12955 12956 CAPS BaGS25E06 12959 12960 CAPS bah38n03 12963 12964 CAPS BaAK38G14 12967 12968 CAPS baal5a06 12971 12972 CAPS basd26d19 12975 12976 CAPS BaSD26D07 12979 12980 SNP BaH50G14 12983 12984 CAPS bah21h17 12987 12988 SNP bags5d21 12991 12992 SNP BaAK34D14 12995 12996 SNP baak18a16 12999 13000 SNP bags4b01 13003 13004 SNP bags38c19 13007 13008 SNP BaGS38J23 13011 13012 CAPS kr42D0208 13015 13016 SNP BaH33A16 13019 13020 CAPS bags10e03 13023 13024 CAPS bags14f17 13027 13028 CAPS BaAK35N11 13031 13032 SNP

TABLE 12-4 Primers used Name (SEQ ID NO:) marker type bags26n10 13035 13036 SNP bags37n02 13039 13040 SNP baal39l05 13043 13044 size_poly (dominant) BaAK26L11 13047 13048 SNP BaH57C19 13051 13052 SNP bags7a01 13055 13056 SNP bah19l15 13059 13060 SNP baal4o02 13063 13064 SNP bah58a12 13067 13068 SNP HVM30 — — SSR bah11b08 13071 13072 SNP bags14m15 13075 13076 SNP baak1e17 13079 13080 CAPS bags14i11 13083 13084 size_poly (codominant) bastl04B1103 13087 13088 SNP bags13g08 13091 13092 SNP basd27p03 13095 13096 SNP bast38D0707 13099 13100 SNP baak35j18 13103 13104 CAPS baal19k05 13107 13108 SNP BaH19C21 13111 13112 SNP bah49g10 13115 13116 SNP bags14h08 13119 13120 CAPS bags23g18 13123 13124 SNP bags22o22 13127 13128 SNP BaAL20A03 13131 13132 SNP bags5e24 13135 13136 SNP bags5f11 13139 13140 SNP BaAK33B10 13143 13144 SNP BaSD15J20 13147 13148 CAPS bags38b22 13151 13152 size_poly (codominant) bah39b06 13155 13156 CAPS bags19j08 13159 13160 CAPS BaGS1G09 13163 13164 CAPS bah63j06 13167 13168 CAPS BaAL39J02 13171 13172 SNP BaSD27H14 13175 13176 SNP BaH50M01 13179 13180 SNP BaSD24E13 13183 13184 SNP baal5j24 13187 13188 CAPS bah15h18 13191 13192 CAPS bast62E0610 13195 13196 CAPS bah28k24 13199 13200 SNP bah47c11 13203 13204 SNP BaSD26M15 13207 13208 SNP bah60o02 13211 13212 SNP BaH50G06 13215 13216 SNP bast15G0913 13219 13220 SNP bah62n12 13223 13224 size_poly (codominant) baal10n23 13227 13228 CAPS bags20c12 13231 13232 SNP

TABLE 12-5 Primers used Name (SEQ ID NO:) marker type basd16f13 13235 13236 SNP baak21h06 13239 13240 CAPS bags3b05 13243 13244 CAPS bags22p08 13247 13248 SNP BaH23N06 13251 13252 SNP bags29c08 13255 13256 SNP BaGS11O23 13259 13260 SNP baak16a11 13263 13264 CAPS baak15n22 13267 13268 CAPS BaAL18J13 13271 13272 SNP bah15m02 13275 13276 SNP baak38d20 13279 13280 CAPS BaSD12L21 13283 13284 CAPS kr68B1103 13287 13288 SNP BaAK28L22 13291 13292 CAPS bags15b10 13295 13296 SNP bags20h21 13299 13300 SNP BaAK29K06 13303 13304 SNP bags21l05 13307 13308 CAPS bah56j14 13311 13312 SNP basd15e02 13315 13316 CAPS bags3j24 13319 13320 SNP bags38m08 13323 13324 SNP bah11m18 13327 13328 CAPS bags35g06 13331 13332 SNP bags37b01 13335 13336 SNP BaAL19F02 13339 13340 SNP BaAL4D09 13343 13344 SNP BaAK30H10 13347 13348 CAPS bags38b16 13351 13352 CAPS bags27f15 13355 13356 SNP bags10k14 13359 13360 SNP bags22f10 13363 13364 CAPS baak38n21 13367 13368 SNP baak30e05 13371 13372 SNP bastl04A0101 13375 13376 SNP BaAK28P18 13379 13380 SNP BaGS34E01 13383 13384 SNP BaH54H04 13387 13388 SNP bah13o19 13391 13392 SNP BaAK29B22 13395 13396 CAPS BaAK27C16 13399 13400 CAPS BaGS22H13 13403 13404 CAPS BaSD27A15 13407 13408 SNP BaAK27E07 13411 13412 CAPS BaGS9N02 13415 13416 SNP Bmag223 — — SSR bags28o18 13419 13420 CAPS baal25b05 13423 13424 CAPS bastl30A0202 13427 13428 SNP bags37d20 13431 13432 CAPS

TABLE 12-6 Primers used Name (SEQ ID NO:) marker type BaGS22K12 13435 13436 SNP BaAK13G12 13439 13440 SNP basd2a02 13443 13444 CAPS BaGS31L06 13447 13448 CAPS bags17j17 13451 13452 CAPS baal12j15 13455 13456 SNP BaAK19H14 13459 13460 CAPS BaH34J11 13463 13464 SNP BaGS30I11 13467 13468 SNP BaH49L06 13471 13472 CAPS baal18m24 13475 13476 CAPS baak15l06 13479 13480 SNP basd18o14 13483 13484 SNP bags24p22 13487 13488 SNP BaAL37A09 13491 13492 SNP bags5l01 13495 13496 SNP BaAK12P03 13499 13500 SNP baak21f10 13503 13504 SNP bastl30E0410 13507 13508 SNP kr25C0206 13511 13512 SNP baak4j01 13515 13516 CAPS BaAK27E01 13519 13520 CAPS bah54n06 13523 13524 SNP bags19p05 13527 13528 CAPS bast22F0311 13531 13532 SNP basd1m04 13535 13536 SNP bags35i04 13539 13540 CAPS baak41d01 13543 13544 SNP baal19m08 13547 13548 CAPS baak13j10 13551 13552 SNP baak44h11 13555 13556 SNP baal19j09 13559 13560 SNP baet37C1105 13563 13564 SNP baak12d06 13567 13568 CAPS basd23f10 13571 13572 SNP BaH41P07 13575 13576 SNP baak1g13 13579 13580 SNP basd14b04 13583 13584 CAPS bags22l14 13587 13588 SNP bast26E1210 13591 13592 SNP baet25F0911 13595 13596 SNP BaAK32N13 13599 13600 SNP BaSD2C09 13603 13604 size_poly (codominant) BaGS31P01 13607 13608 SNP baak30m11 13611 13612 SNP BaAK4N16 13615 13616 size_poly (codominant) bastl46F1012 13619 13620 SNP bags3e06 13623 13624 SNP bags31o10 13627 13628 CAPS bags33j12 13631 13632 CAPS BaGS30N07 13635 13636 CAPS

TABLE 12-7 Primers used Name (SEQ ID NO:) marker type BaH37I18 13639 13640 CAPS baak46p24 13643 13644 CAPS baak46o19 13647 13648 SNP basd3c19 13651 13652 SNP bags30f01 13655 13656 SNP BaGS20F10 13659 13660 CAPS BaGS32D08 13663 13664 SNP BaAK23M23 13667 13668 SNP bah21a16 13671 13672 SNP BaAL3M08 13675 13676 SNP BaAK21I09 13679 13680 SNP baet33A0301 13683 13684 SNP baak24k02 13687 13688 SNP bah33p03 13691 13692 CAPS bast75D1208 13695 13696 SNP BaSD12K20 13699 13700 size_poly (codominant) bags34f06 13703 13704 SNP bags6f09 13707 13708 SNP bags7b10 13711 13712 SNP BaGS25H01 13715 13716 CAPS baak33m08 13719 13720 CAPS bags37e01 13723 13724 SNP bags32n20 13727 13728 CAPS BaH47A11 13731 13732 CAPS BaGS24M06 13735 13736 SNP bags22m23 13739 13740 SNP basd18d18 13743 13744 SNP bastl06F0212 13747 13748 SNP BaH49O16 13751 13752 SNP bags39e22 13755 13756 CAPS BaH38N06 13759 13760 SNP BaH56P16 13763 13764 SNP BaSD13O13 13767 13768 SNP bags19i06 13771 13772 CAPS bah34f11 13775 13776 SNP bags37g04 13779 13780 SNP basd11k21 13783 13784 CAPS HvLOX — — SSR baak43o03 13787 13788 SNP BaAL4J21 13791 13792 SNP BaH51J22 13795 13796 SNP bah58l03 13799 13800 SNP BaGS21M18 13803 13804 SNP BaGS31K06 13807 13808 SNP baal4o01* 13811 13812 SNP VRN2(sh2) BaAK24H19 13815 13816 size_poly (codominant) BaGS17D21 13819 13820 size_poly (codominant) bah39o04 13823 13824 SNP baak46e06 13827 13828 size_poly (codominant) basd13j03 13831 13832 CAPS

TABLE 12-8 Primers used Name (SEQ ID NO:) marker type bast14H0416 13835 13836 SNP BaSD18I03 13839 13840 CAPS bastl44H1115 13843 13844 SNP bast34D0608 13847 13848 CAPS BaGS30F06 13851 13852 CAPS bags21k16 13855 13856 CAPS BaH48G01 13859 13860 CAPS bags39a24 13863 13864 CAPS bags15i03 13867 13868 size_poly (dominant) bags23f02 13871 13872 SNP bastl40E1010 13875 13876 SNP bags17p10 13879 13880 CAPS BaH57L13 13883 13884 CAPS bags10e22 13887 13888 size_poly (codominant) bags4p07 13891 13892 CAPS bah63a08 13895 13896 CAPS baak12f13 13899 13900 size_poly (dominant) bah56j15 13903 13904 CAPS bah15e16 13907 13908 SNP BaH50F21 13911 13912 CAPS basd27o16 13915 13916 SNP kr66G0713 13919 13920 SNP baak46c17 13923 13924 SNP baet19C1206 13927 13928 SNP bags34e15 13931 13932 CAPS baal4d18 13935 13936 size_poly (dominant) bah13b13 13939 13940 CAPS BaGS26G20 13943 13944 SNP BaGS28C14 13947 13948 SNP kr61G1214 13951 13952 SNP bags9g08 13955 13956 SNP bags5p01 13959 13960 SNP BaAL4D04 13963 13964 SNP bah26p09 13967 13968 CAPS bah11e02 13971 13972 SNP MWG2077 — — STS bast41F0311 13975 13976 SNP bastl54D0307 13979 13980 SNP BaSD3F21 13983 13984 size_poly (codominant) bah56f18 13987 13988 size_poly (codominant) BaH41C21 13991 13992 CAPS basd19g21 13995 13996 CAPS kr33H0816 13999 14000 SNP BaH38F16 14003 14004 SNP BaGS21P09 14007 14008 SNP BaGS36M20 14011 14012 CAPS bags21m10 14015 14016 CAPS BaAK14G11 14019 14020 CAPS BaGS30L20 14023 14024 CAPS basd21g02 14027 14028 SNP bah22m17 14031 14032 SNP

TABLE 12-9 Primers used Name (SEQ ID NO:) marker type BaGS9H19 14035 14036 SNP bah26m24 14039 14040 SNP BaH56D01 14043 14044 SNP bah53j08 14047 14048 CAPS bags21d10 14051 14052 CAPS bags9a01 14055 14056 CAPS baal9m13 14059 14060 SNP baak20m05 14063 14064 size_poly (codominant) baak21k16 14067 14068 SNP bags34d17 14071 14072 CAPS baal40k24 14075 14076 CAPS BaGS19O14 14079 14080 SNP baak35m03 14083 14084 SNP baak33o23 14087 14088 SNP kr57F1012 14091 14092 SNP bah53i05 14095 14096 size_poly (codominant) bags22l16 14099 14100 SNP baet43C0505 14103 14104 SNP BaH33H02 14107 14108 CAPS bastl22E1010 14111 14112 SNP BaH53N24 14115 14116 SNP baal8e24 14119 14120 SNP BaH62H21 14123 14124 SNP BaH42K01 14127 14128 SNP basd26p09 14131 14132 SNP bags4p18 14135 14136 SNP BaH60H14 14139 14140 size_poly (codominant) bast70D1107 14143 14144 SNP bah26i23 14147 14148 SNP baal39g02 14151 14152 SNP bags10e02 14155 14156 CAPS bags22c13 14159 14160 SNP bags35n03 14163 14164 CAPS BaAL16H03 14167 14168 SNP BaAL15N07 14171 14172 CAPS bags18o20 14175 14176 SNP bags37g12 14179 14180 CAPS bags22l12 14183 14184 CAPS BaAK44K01 14187 14188 CAPS BaAK31G07 14191 14192 CAPS bah53n21 14195 14196 CAPS BaGS19C02 14199 14200 SNP BaH52E20 14203 14204 SNP BaAL4N05 14207 14208 SNP bags7p06 14211 14212 SNP bast70C0905 14215 14216 SNP BaSD15J01 14219 14220 SNP BaH56H18 14223 14224 SNP BaGS4N04 14227 14228 SNP bags31o13 14231 14232 SNP kr29D0107 14235 14236 CAPS

TABLE 12-10 Primers used Name (SEQ ID NO:) marker type BaAK21A15 14239 14240 size_poly (codominant) bags7h14 14243 14244 SNP basd26b20 14247 14248 size_poly (codominant) baal1g06 14251 14252 size_poly (codominant) bags5n14 14255 14256 SNP BaAK34H17 14259 14260 size_poly (dominant) bastl32H0816 14263 14264 SNP basd18m12 14267 14268 size_poly (codominant) baak31p06 14271 14272 SNP bah52i03 14275 14276 CAPS BaAK33K05 14279 14280 CAPS BaAK44J08 14283 14284 SNP baak18g16 14287 14288 SNP baak11a11 14291 14292 SNP BaH25B20 14295 14296 size_poly (codominant) bast12G0113 14299 14300 CAPS baal9n02 14303 14304 SNP bags20f08 14307 14308 SNP BaGS9C24 14311 14312 SNP BaGS22O02 14315 14316 SNP baak13k16 14319 14320 CAPS MWG2249 — — STS bah41b17 14323 14324 SNP bah63m11 14327 14328 SNP BaGS28P07 14331 14332 SNP bags1h15 14335 14336 CAPS kr26H0515 14339 14340 CAPS bags6n05 14343 14344 size_poly (codominant) BaH24P17 14347 14348 CAPS bast03D0408 14351 14352 SNP BaAL17G06 14355 14356 SNP BaH32G11 14359 14360 CAPS BaGS4P17 14363 14364 SNP baal31o21 14367 14368 SNP BaGS36A16 14371 14372 CAPS bags4d11 14375 14376 SNP basd13n18 14379 14380 SNP basd0a08 14383 14384 SNP BaGS32P24 14387 14388 SNP baak12i02 14391 14392 CAPS kr06H0315 14395 14396 SNP BaAL26B09 14399 14400 SNP kr32C1105 14403 14404 SNP BaH53B15 14407 14408 SNP bags13h04 14411 14412 SNP baal15i11 14415 14416 SNP bags1m19 14419 14420 CAPS HVM6 — — SSR

TABLE 13-1 Primers used Name (SEQ ID NO:) marker type MWG620 — — STS basd14f03 14423 14424 CAPS BaH30E11 14427 14428 SNP BaH59J05 14431 14432 SNP BaAL39O22 14435 14436 SNP baal16i24 14439 14440 CAPS BaAL30P12 14443 14444 CAPS BaGS21H12 14447 14448 CAPS BaAK15J05 14451 14452 size_poly (codominant) BaGS9G15 14455 14456 CAPS BaAK41J04 14459 14460 CAPS baak43j24 14463 14464 SNP bah26n19 14467 14468 size_poly (dominant) Bmac316 — — SSR bags38a05 14471 14472 CAPS BaH43I06 14475 14476 CAPS bags9j07 14479 14480 CAPS baak31p11 14483 14484 CAPS BaGS17P19 14487 14488 CAPS baal32j05 14491 14492 CAPS baal16l16 14495 14496 CAPS baal6a11 14499 14500 CAPS bah55p06 14503 14504 CAPS bags8f03 14507 14508 CAPS bags3m02 14511 14512 SNP BaAL36L08 14515 14516 SNP baet19E0309 14519 14520 SNP BaSD18J06 14523 14524 size_poly (codominant) bast48D0307 14527 14528 SNP kr55D0707 14531 14532 SNP BaAL4B09 14535 14536 CAPS bags32f21 14539 14540 CAPS BaGS24F04 14543 14544 SNP BaGS10G07 14547 14548 CAPS BaGS26P07 14551 14552 SNP bags12f11 14555 14556 SNP MWG2218 — — STS BaH63O04 14559 14560 CAPS BaGS26D21 14563 14564 CAPS bags20o14 14567 14568 SNP baak36d08 14571 14572 SNP bast75H0616 14575 14576 SNP basd16g17 14579 14580 SNP baal4k16 14583 14584 CAPS bags32b14 14587 14588 CAPS baal4d01 14591 14592 CAPS baal7d23 14595 14596 CAPS BaH62C20 14599 14600 size_poly (codominant) BaH21K13 14603 14604 SNP BaAK14J02 14607 14608 SNP bah22c08 14611 14612 SNP

TABLE 13-2 Primers used Name (SEQ ID NO:) marker type baak25d07 14615 14616 CAPS BaAK29M05 14619 14620 CAPS BaGS31I08 14623 14624 CAPS bah12n12 14627 14628 SNP baal30o06 14631 14632 CAPS baak13n14 14635 14636 CAPS bags7a09 14639 14640 CAPS baak32l04 14643 14644 SNP bah31o16 14647 14648 CAPS BaAL7C19 14651 14652 SNP BaAK23F04 14655 14656 SNP kr40D0707 14659 14660 CAPS basd0g08 14663 14664 SNP BaAL30H03 14667 14668 CAPS BaAL21B21 14671 14672 SNP BaGS30E05 14675 14676 SNP BaAK13G04 14679 14680 SNP BaGS27N11 14683 14684 size_poly (dominant) BaGS9A04 14687 14688 SNP BaAK23M11 14691 14692 SNP basd3f22 14695 14696 CAPS baak41p21 14699 14700 SNP BaAK41K07 14703 14704 CAPS BaH53I11 14707 14708 CAPS baak26j14 14711 14712 SNP BaAL18N03 14715 14716 CAPS baak40c08 14719 14720 CAPS bags17l19 14723 14724 CAPS BaSD25N02 14727 14728 size_poly (codominant) BaAK37E04 14731 14732 SNP baal33h17 14735 14736 CAPS bags20d08 14739 14740 CAPS BaGS4J01 14743 14744 CAPS bah48o03 14747 14748 SNP bastl31A0901 14751 14752 SNP bah20k22 14755 14756 SNP BaGS15J17 14759 14760 size_poly (dominant) BaAK17I01 14763 14764 CAPS BaAK30F08 14767 14768 CAPS bah47a17 14771 14772 SNP basd15e10 14775 14776 SNP BaH56C11 14779 14780 SNP BaH18C17 14783 14784 SNP BaGS20G24 14787 14788 SNP bags19g17 14791 14792 CAPS BaAK21L02 14795 14796 CAPS BaH52H21 14799 14800 SNP bags9l13 14803 14804 CAPS BaSD18C12 14807 14808 SNP baak23k07 14811 14812 SNP bags16j16 14815 14816 SNP

TABLE 13-3 Primers used Name (SEQ ID NO:) marker type BaSD15O17 14819 14820 SNP baak12i20 14823 14824 CAPS BaAK3M04 14827 14828 CAPS BaGS4N23 14831 14832 SNP baak34k24 14835 14836 CAPS bah15n23 14839 14840 CAPS baak45i02 14843 14844 CAPS basd13i08 14847 14848 SNP BaSD17P01 14851 14852 SNP HVM31 — — SSR baak13d19 14855 14856 SNP baal3h14 14859 14860 SNP bags33j18 14863 14864 SNP BaH45B01 14867 14868 CAPS baak21n07 14871 14872 SNP BaH36H18 14875 14876 size_poly (codominant) BaAL23O14 14879 14880 CAPS bah53f05 14883 14884 SNP baak12d02 14887 14888 SNP bags11h12 14891 14892 SNP baet01F1212 14895 14896 SNP baak17l11 14899 14900 SNP basd20e17 14903 14904 SNP bah29p24 14907 14908 SNP bags30o05 14911 14912 SNP kr68D0208 14915 14916 SNP bags37l11 14919 14920 SNP baal4d14 14923 14924 CAPS BaH58I23 14927 14928 SNP bags39l04 14931 14932 SNP baak29j13 14935 14936 CAPS bags20p18 14939 14940 CAPS BaAK12J13 14943 14944 CAPS BaH13K17 14947 14948 size_poly (codominant) bah60p09 14951 14952 SNP BaH27N11 14955 14956 CAPS BaGS34D11 14959 14960 CAPS BaGS39G07 14963 14964 CAPS bah22o14 14967 14968 SNP bah14l20 14971 14972 SNP bah42p22 14975 14976 SNP BaAK21G03 14979 14980 SNP BaAL35D24 14983 14984 SNP baak45h14 14987 14988 SNP bags28o05 14991 14992 SNP BaAK31P07 14995 14996 SNP bah36c06 14999 15000 SNP BaGS37H24 15003 15004 SNP bast65B0303 15007 15008 SNP BaH51A22 15011 15012 SNP BaGS39K23 15015 15016 SNP

TABLE 13-4 Primers used Name (SEQ ID NO:) marker type BaSD14O12 15019 15020 SNP BaH57F12 15023 15024 SNP BaGS32G02 15027 15028 CAPS BaAL40N06 15031 15032 SNP bah11b14 15035 15036 SNP bah15j14 15039 15040 SNP bast21C1105 15043 15044 CAPS bags29f03 15047 15048 CAPS BaAK46B16 15051 15052 CAPS basd11p22 15055 15056 CAPS baal16h16 15059 15060 CAPS BaSD21L07 15063 15064 SNP BaH42D07 15067 15068 SNP BaGS23K24 15071 15072 SNP BaAK24L01 15075 15076 SNP BaAL11H22 15079 15080 SNP BaAL13O24 15083 15084 CAPS BaAL6M22 15087 15088 SNP BaH56I06 15091 15092 size_poly (codominant) BaSD19A18 15095 15096 size_poly (dominant) BaSD20P03 15099 15100 SNP BaH19B13 15103 15104 SNP kr59H0416 15107 15108 SNP BaGS14A02 15111 15112 SNP BaH34P05 15115 15116 SNP bah61o16 15119 15120 SNP BaH24N07 15123 15124 SNP BaAL20M22 15127 15128 CAPS BaAL11H20 15131 15132 SNP bah54b04 15135 15136 SNP BaAK14H23 15139 15140 size_poly (dominant) BaSD20M23 15143 15144 SNP BaAL15B12 15147 15148 CAPS baal15d09 15151 15152 SNP baak39n20 15155 15156 SNP BaH43N16 15159 15160 SNP bags39h18 15163 15164 CAPS BaAL3L23 15167 15168 CAPS basd22c03 15171 15172 SNP bast55G0913 15175 15176 CAPS kr47F0511 15179 15180 SNP bah15l24 15183 15184 size_poly (codominant) bast77A0402 15187 15188 SNP BaH58F04 15191 15192 SNP BaAK30L09 15195 15196 SNP kr39G1113 15199 15200 SNP baak1c16 15203 15204 SNP basd20e05 15207 15208 SNP BaAK12F18 15211 15212 SNP BaH50L11 15215 15216 CAPS bast50G0814 15219 15220 CAPS

TABLE 13-5 Primers used Name (SEQ ID NO:) marker type bast70D0107 15223 15224 SNP baal17d05 15227 15228 SNP baak46g03 15231 15232 SNP bah63m17 15235 15236 SNP BaH15L15 15239 15240 SNP BaGS19N01 15243 15244 SNP BaAK32P07 15247 15248 SNP bags14l13 15251 15252 SNP bags37o24 15255 15256 SNP baal5e24 15259 15260 CAPS BaH46B06 15263 15264 CAPS bags11a16 15267 15268 CAPS baal27m03 15271 15272 SNP BaAK1C18 15275 15276 SNP BaAK38H16 15279 15280 size_poly (codominant) BaGS37K02 15283 15284 CAPS bah28a10 15287 15288 CAPS bah27c03 15291 15292 CAPS BaH52B11 15295 15296 SNP bah27f05 15299 15300 SNP bags37g15 15303 15304 CAPS bah59c10 15307 15308 SNP bags38j06 15311 15312 SNP basd14e01 15315 15316 SNP BaGS24L06 15319 15320 size_poly (codominant) bags17b02 15323 15324 CAPS BaH54L03 15327 15328 CAPS BaAK21J17 15331 15332 CAPS BaGS23G08 15335 15336 CAPS basd18e06 15339 15340 SNP baak19o02 15343 15344 size_poly (codominant) basd2o16 15347 15348 SNP baak16e08 15351 15352 SNP bags18h01 15355 15356 SNP BaGS4L20 15359 15360 SNP BaAL2M19 15363 15364 CAPS BaH36F15 15367 15368 CAPS bah58l07 15371 15372 CAPS baet29C0406 15375 15376 CAPS BaAK35B04 15379 15380 CAPS bags34k13 15383 15384 CAPS baal29i08 15387 15388 CAPS baak11p10 15391 15392 CAPS BaH30J08 15395 15396 SNP bags4e12 15399 15400 CAPS bastl05C1206 15403 15404 size_poly (codominant) bast22G0313 15407 15408 SNP basd24b08 15411 15412 CAPS bastl16C0206 15415 15416 size_poly (codominant) bags33n21 15419 15420 SNP baak33c11 15423 15424 SNP

TABLE 13-6 Primers used Name (SEQ ID NO:) marker type baal13o10 15427 15428 CAPS BaH36P06 15431 15432 SNP basd12l01 15435 15436 SNP baet25H0115 15439 15440 SNP bags15d14 15443 15444 CAPS BaGS37E09 15447 15448 CAPS BaAK29I15 15451 15452 SNP BaAK18I01 15455 15456 SNP bast62B0404 15459 15460 SNP bah25l03 15463 15464 SNP BaGS19I11 15467 15468 CAPS baal1n11 15471 15472 SNP baak16l07 15475 15476 SNP BaGS18P04 15479 15480 CAPS bags19e06 15483 15484 CAPS baak35n07 15487 15488 CAPS BaAK29K23 15491 15492 SNP baal13c18 15495 15496 SNP bags7d17 15499 15500 SNP bags20j08 15503 15504 SNP baal9c20 15507 15508 SNP bags20l05 15511 15512 SNP bah63k05 15515 15516 size_poly (codominant) BaAK14C17 15519 15520 CAPS Bmac40 — — SSR baal15j23 15523 15524 SNP BaAK1N06 15527 15528 CAPS bags38d10 15531 15532 CAPS BaAK19J15 15535 15536 CAPS BaH50L14 15539 15540 SNP BaGS37E11 15543 15544 SNP BaSD27E20 15547 15548 SNP basd17f18 15551 15552 SNP baal32m04 15555 15556 SNP BaSD12L12 15559 15560 CAPS BaGS37D24 15563 15564 CAPS BaGS9K15 15567 15568 CAPS BaGS33L03 15571 15572 CAPS BaAK24G10 15575 15576 SNP kr58F0511 15579 15580 CAPS BaH50F16 15583 15584 SNP basd17d11 15587 15588 SNP BaH37P24 15591 15592 SNP bah47l21 15595 15596 SNP baet45C1105 15599 15600 SNP baak39o18 15603 15604 CAPS bags20p12 15607 15608 CAPS bags38f23 15611 15612 CAPS baak14b08 15615 15616 SNP bah54n10 15619 15620 SNP bags3k09 15623 15624 CAPS

TABLE 13-7 Primers used Name (SEQ ID NO:) marker type bags34p08 15627 15628 SNP baal1e06 15631 15632 SNP baak34p14 15635 15636 CAPS baak14k12 15639 15640 CAPS BaSD13G17 15643 15644 size_poly (codominant) MWG897 — — STS bags27h05 15647 15648 size_poly (codominant) basd15m11 15651 15652 CAPS BaH28G09 15655 15656 CAPS bags21b06 15659 15660 CAPS basd12g17 15663 15664 size_poly (codominant) BaH18F07 15667 15668 CAPS BaH44A23 15671 15672 CAPS bags3d07 15675 15676 CAPS BaAL6A21 15679 15680 SNP BaAK24E07 15683 15684 CAPS BaAL29L09 15687 15688 SNP kr66D1107 15691 15692 SNP bastl47C0606 15695 15696 SNP BaH50M03 15699 15700 SNP basd27k17 15703 15704 SNP

TABLE 14-1 Primers used Name (SEQ ID NO:) marker type basd2p19 15707 15708 SNP BaAK38B18 15711 15712 size_poly (dominant) bah42i06 15715 15716 CAPS bags6f02 15719 15720 SNP BaAK39J07 15723 15724 SNP BaAK1L23 15727 15728 CAPS baak17e10 15731 15732 SNP BaH50H08 15735 15736 SNP baal17e10 15739 15740 SNP BaSD17C05 15743 15744 SNP BaGS14P02 15747 15748 SNP bastl42H0115 15751 15752 SNP BaAK19C08 15755 15756 SNP bastl40H1115 15759 15760 SNP bags12l21 15763 15764 CAPS baak12j24 15767 15768 CAPS bags14d22 15771 15772 CAPS BaAK21P12 15775 15776 SNP baal6b02 15779 15780 CAPS baak29f13 15783 15784 CAPS bast79C0406 15787 15788 SNP BaH45M08 15791 15792 SNP kr28A0301 15795 15796 SNP bags19e04 15799 15800 CAPS BaH28L07 15803 15804 CAPS baak21m18 15807 15808 CAPS baak21l22 15811 15812 SNP basd15h01 15815 15816 SNP BaH19O11 15819 15820 SNP BaH59A20 15823 15824 CAPS bags39l05 15827 15828 SNP BaAK31N06 15831 15832 CAPS BaH58P03 15835 15836 SNP bags29c18 15839 15840 CAPS BaAL4O04 15843 15844 SNP bags37n23 15847 15848 CAPS BaAL39N03 15851 15852 size_poly (codominant) BaAK14C23 15855 15856 SNP kr61A1101 15859 15860 SNP BaH42J17 15863 15864 SNP BaH58H08 15867 15868 SNP bast78A0202 15871 15872 SNP BaGS21E20 15875 15876 CAPS bags22k18 15879 15880 size_poly (codominant) BaGS33H15 15883 15884 size_poly (codominant) HVM4 — — SSR BaSD18O16 15887 15888 SNP baak22j11 15891 15892 SNP BaAK20K04 15895 15896 SNP bags29l11 15899 15900 SNP BaAK17G18 15903 15904 size_poly (dominant)

TABLE 14-2 Primers used Name (SEQ ID NO:) marker type baak14o13 15907 15908 SNP baal8d17 15911 15912 size_poly (codominant) BaAK37P20 15915 15916 CAPS bags28k04 15919 15920 SNP bah34b22 15923 15924 CAPS BaGS37M03 15927 15928 SNP BaGS18C14 15931 15932 SNP bast23G0214 15935 15936 SNP bah47p02 15939 15940 SNP baak42a24 15943 15944 CAPS baak16f06 15947 15948 CAPS bah11m12 15951 15952 CAPS kr66H0216 15955 15956 SNP BaGS23D15 15959 15960 SNP baal22c17 15963 15964 SNP baak33d06 15967 15968 CAPS bags34l17 15971 15972 SNP BaGS25G03 15975 15976 CAPS bah14j16 15979 15980 CAPS bah34a23 15983 15984 size_poly (codominant) BaAK21B17 15987 15988 SNP bast42C0406 15991 15992 CAPS baak21m13 15995 15996 SNP baet45H0115 15999 16000 size_poly (codominant) BaH38E01 16003 16004 SNP bast03G0313 16007 16008 SNP baak38p18 16011 16012 size_poly (codominant) basd18g06 16015 16016 SNP baak40i22 16019 16020 SNP baal2n12 16023 16024 SNP BaSD3C24 16027 16028 SNP BaAK46M07 16031 16032 SNP BaGS17N04 16035 16036 SNP bags12d09 16039 16040 SNP basd20c08 16043 16044 SNP bags17i04 16047 16048 CAPS baak15p03 16051 16052 CAPS baak24b20 16055 16056 size_poly (codominant) baal18g11 16059 16060 SNP baal40b06 16063 16064 CAPS baak40g02 16067 16068 SNP baal1m11 16071 16072 size_poly (codominant) BaH26B16 16075 16076 SNP baal5n08 16079 16080 SNP BaSD27F05 16083 16084 size_poly (codominant) bags9c08 16087 16088 SNP baak3c01 16091 16092 CAPS basd1j22 16095 16096 SNP BaH23N03 16099 16100 SNP bast46D0808 16103 16104 SNP bast22A0802 16107 16108 SNP

TABLE 14-3 Primers used Name (SEQ ID NO:) marker type bast34D0808 16111 16112 SNP bast48E0509 16115 16116 SNP bast26F1012 16119 16120 SNP baal30e07 16123 16124 SNP bags23n06 16127 16128 CAPS BaGS18I05 16131 16132 SNP BaH52L11 16135 16136 CAPS baal18b16 16139 16140 CAPS bags1a17 16143 16144 size_poly (dominant) HVCMA — — SSR bags8o06 16147 16148 SNP baak46e14 16151 16152 SNP BaGS38N08 16155 16156 SNP BaH49P17 16159 16160 SNP bah60l22 16163 16164 CAPS bags21m22 16167 16168 CAPS BaH54E07 16171 16172 CAPS BaAK39A20 16175 16176 CAPS bags21d11 16179 16180 SNP kr39H0816 16183 16184 SNP baet32B1103 16187 16188 CAPS BaAK31O16 16191 16192 CAPS bags39l20 16195 16196 CAPS BaGS15L12 16199 16200 CAPS kr67D0208 16203 16204 SNP BaAK1H16 16207 16208 SNP BaH26F10 16211 16212 SNP bags14n02 16215 16216 SNP bah36f01 16219 16220 CAPS bast79F0711 16223 16224 size_poly (codominant) BaGS29G21 16227 16228 size_poly (codominant) bast79D1107 16231 16232 SNP BaGS26O20 16235 16236 SNP bags11h08 16239 16240 CAPS bags22h11 16243 16244 SNP baet19D0608 16247 16248 SNP bags23a14 16251 16252 CAPS cMWG704 — — STS bah62d14 16255 16256 size_poly (codominant) BaGS37C09 16259 16260 SNP bags20a01 16263 16264 SNP bah53m11 16267 16268 SNP BaGS26L01 16271 16272 SNP bags21p23 16275 16276 SNP BaAK24B01 16279 16280 SNP BaAK13L03 16283 16284 CAPS MWG511 — — CAPS bastl56C0105 16287 16238 SNP basd20j22 16291 16292 CAPS bags28d11 16295 16296 SNP BaGS4I11 16299 16300 size_poly (codominant)

TABLE 14-4 Primers used Name (SEQ ID NO:) marker type BaH52G15 16303 16304 size_poly (codominant) baak11d12 16307 16308 SNP BaH42A07 16311 16312 SNP BaGS38F01 16315 16316 CAPS BaAK32I05 16319 16320 CAPS bah60f18 16323 16324 SNP baal2n10 16327 16328 CAPS BaAK14F01 16331 16332 CAPS bah57o01 16335 16336 CAPS basd12f05 16339 16340 CAPS bah58e21 16343 16344 CAPS bags1d07 16347 16348 CAPS BaH23J15 16351 16352 CAPS baak1p09 16355 16356 CAPS BaAK32C23 16359 16360 SNP baal9e06 16363 16364 SNP baal18g23 16367 16368 CAPS bastl47E0309 16371 16372 SNP baak30o08 16375 16376 SNP baet44C0606 16379 16380 SNP bags39g18 16383 16384 SNP BaH50P14 16387 16388 CAPS bags38m06 16391 16392 SNP baal33e12 16395 16396 size_poly (codominant) bags3i04 16399 16400 SNP BaAL1A11 16403 16404 SNP BaSD2M23 16407 16408 SNP Bmag359 — — SSR BaAK23L23 16411 16412 CAPS bags7a23 16415 16416 CAPS bah56l04 16419 16420 CAPS basd12k23 16423 16424 SNP bast22C0105 16427 16428 SNP BaGS38H20 16431 16432 SNP bah61p21 16435 16436 SNP basd11h13 16439 16440 SNP BaAL41J12 16443 16444 size_poly (codominant) baal0f04 16447 16448 SNP kr18E0810 16451 16452 SNP BaH15J07 16455 16456 size_poly (codominant) BaAL6G08 16459 16460 CAPS BaGS24F02 16463 16464 SNP baal19m12 16467 16468 CAPS bah34h16 16471 16472 size_poly (codominant) baak2e06 16475 16476 SNP bast10C0406 16479 16480 CAPS baak12l16 16483 16484 SNP baak29d01 16487 16488 SNP BaAK19N06 16491 16492 SNP BaAK4I02 16495 16496 SNP BaH49K10 16499 16500 SNP

TABLE 14-5 Primers used Name (SEQ ID NO:) marker type baal2o03 16503 16504 SNP baak28g14 16507 16508 SNP bah62n05 16511 16512 CAPS bags16e11 16515 16516 SNP bags23g13 16519 16520 SNP BaSD23C05 16523 16524 SNP BaH19F17 16527 16528 SNP BaH15J02 16531 16532 size_poly (codominant) baak20d11 16535 16536 CAPS BaAL6D19 16539 16540 CAPS baak43e16 16543 16544 CAPS baak40b02 16547 16548 CAPS bah62m03 16551 16552 CAPS BaGS26G17 16555 16556 SNP baal6j16 16559 16560 SNP bags29b09 16563 16564 SNP baal20e03 16567 16568 SNP BaGS9L23 16571 16572 SNP bah32e22 16575 16576 SNP BaAK17O14 16579 16580 SNP bah56g23 16583 16584 CAPS basd22f14 16587 16588 CAPS BaH42O04 16591 16592 SNP bast74F0111 16595 16596 SNP BaSD21F13 16599 16600 SNP BaAK26G13 16603 16604 CAPS BaAK44D07 16607 16608 CAPS bast61C0206 16611 16612 size_poly (codominant) BaAL29L08 16615 16616 SNP BaH34B20 16619 16620 CAPS baak21a04 16623 16624 SNP BaGS37F18 16627 16628 SNP baal12h12 16631 16632 SNP BaAK42B16 16635 16636 CAPS BaAK26F12 16639 16640 SNP BaH27B21 16643 16644 size_poly (codominant) BaGS12B05 16647 16648 SNP baal12h24 16651 16652 SNP baal35j16 16655 16656 SNP BaGS37C07 16659 16660 SNP BaAK45D23 16663 16664 SNP BaH42G09 16667 16668 SNP BaAL40P01 16671 16672 SNP kr61A0901 16675 16676 SNP baal4d05 16679 16680 CAPS basd20l03 16683 16684 CAPS baal31b03 16687 16688 CAPS bags27k21 16691 16692 CAPS bah55n15 16695 16696 CAPS BaH44B17 16699 16700 SNP BaSD2K11 16703 16704 SNP

TABLE 14-6 Primers used Name (SEQ ID NO:) marker type BaAK20B23 16707 16708 CAPS BaGS23E20 16711 16712 SNP bags33i10 16715 16716 SNP bags35k06 16719 16720 SNP bags22c18 16723 16724 CAPS MWG2031 — — STS BaGS22M17 16727 16728 SNP baak21j24 16731 16732 SNP BaH29D20 16735 16736 CAPS bah44f20 16739 16740 CAPS BaAL16O08 16743 16744 SNP BaAL27L17 16747 16748 SNP sKT3 — — size_poly sKT9 — — size_poly baet31B1103 16751 16752 SNP MWG975 — — STS BaAL4A11 16755 16756 CAPS BaGS22I18 16759 16760 CAPS BaGS24K08 16763 16764 SNP bah16c17 16767 16768 SNP bah42m04 16771 16772 size_poly (codominant) bags28l21 16775 16776 SNP basd12f23 16779 16780 size_poly (codominant) bah31m22 16783 16784 SNP BaAL30C17 16787 16788 SNP BaAK40P18 16791 16792 CAPS baak37n01 16795 16796 SNP BaGS24O11 16799 16800 SNP BaH27K19 16803 16804 CAPS BaGS15J07 16807 16808 CAPS bah19a07 16811 16812 CAPS BaH50I23 16815 16816 SNP BaH56D06 16819 16820 SNP BaAK39M05 16823 16824 SNP bah11m16 16827 16828 SNP bah49d03 16831 16832 SNP bah62l23 16835 16836 SNP BaAK38J13 16839 16840 SNP BaH37N04 16843 16844 SNP basd27c06 16847 16848 SNP bah39g10 16851 16852 SNP BaSD18P05 16855 16856 size_poly (codominant) BaGS20A13 16859 16860 SNP bah44n05 16863 16864 CAPS EBmac764 — — SSR BaH27L15 16867 16868 SNP BaAK21I02 16871 16872 size_poly (codominant) Bmac0064 — — SSR BaH59F07 16875 16876 SNP bags9l05 16879 16880 SNP BaGS13H12 16883 16884 CAPS

TABLE 14-7 Primers used Name (SEQ ID NO:) marker type BaAL19B06 16887 16888 CAPS BaSD15P23 16891 16892 CAPS bah53j21 16895 16896 SNP bah21f16 16899 16900 SNP baet39E0309 16903 16904 SNP bags5l04 16907 16908 SNP bags7g10 16911 16912 SNP BaGS9D22 16915 16916 SNP kr27H1216 16919 16920 CAPS BaAK25O11 16923 16924 CAPS bah18j14 16927 16928 SNP bags37k14 16931 16932 SNP baak26n19 16935 16936 size_poly (codominant) bah42k03 16939 16940 SNP BaH36N15 16943 16944 CAPS Bmag120 — — SSR bags12i02 16947 16948 SNP BaAK38E05 16951 16952 CAPS baet25B0604 16955 16956 SNP BaAK46E21 16959 16960 SNP BaGS37J12 16963 16964 SNP bast60C0105 16967 16968 CAPS baak30b08 16971 16972 SNP BaAL29N13 16975 16976 size_poly (codominant) BaAL24O04 16979 16980 CAPS baak20o12 16983 16984 SNP basd14k23 16987 16988 SNP BaAK23L05 16991 16992 SNP BaGS31G02 16995 16996 CAPS bags33o01 16999 17000 SNP basd18g14 17003 17004 CAPS bah24d24 17007 17008 SNP BaGS12F09 17011 17012 SNP BaAK33H23 17015 17016 SNP bah47p03 17019 17020 SNP BaSD14F09 17023 17024 SNP bah11k13 17027 17028 CAPS BaSD22F10 17031 17032 SNP bags32a01 17035 17036 CAPS bags27o20 17039 17040 SNP baak31o10 17043 17044 SNP bags10f16 17047 17048 SNP baal37j12 17051 17052 CAPS BaGS9H02 17055 17056 SNP BaAK40H10 17059 17060 CAPS bah56m09 17063 17064 size_poly (codominant) kr66C0705 17067 17068 SNP BaGS33M05 17071 17072 SNP bast60B0204 17075 17076 size_poly (codominant) bah56p08 17079 17080 CAPS baak12e19 17083 17084 CAPS

TABLE 14-8 Primers used Name (SEQ ID NO:) marker type BaGS1D05 17087 17088 CAPS BaH29M21 17091 17092 CAPS bags21c04 17095 17096 CAPS baak32p09 17099 17100 CAPS BaAL24B02 17103 17104 SNP BaGS34F08 17107 17108 SNP bast36D1208 17111 17112 SNP kr25B1103 17115 17116 SNP kr08D0303 17119 17120 size_poly (dominant) baal19g05 17123 17124 SNP BaH22B15 17127 17128 CAPS bags19c10 17131 17132 SNP bags29k01 17135 17136 SNP bastl54G0414 17139 17140 SNP BaGS23J01 17143 17144 CAPS bags34b15 17147 17148 SNP baak46i07 17151 17152 CAPS BaAL6J13 17155 17156 CAPS bah31l17 17159 17160 SNP BaAK32A05 17163 17164 SNP BaGS30N22 17167 17168 SNP BaH54J20 17171 17172 CAPS BaGS7F20 17175 17176 CAPS bah63p18 17179 17180 size_poly (codominant) bags11p11 17183 17184 CAPS BaH27L17 17187 17188 SNP basd17e11 17191 17192 SNP bags15i06 17195 17196 SNP BaAK36F09 17199 17200 SNP bast57A0101 17203 17204 SNP baak26h17 17207 17208 CAPS basd13b15 17211 17212 SNP bags9d11 17215 17216 SNP BaH20K03 17219 17220 size_poly (codominant) BaSD27N21 17223 17224 size_poly (codominant) baal13a10 17227 17228 size_poly (dominant) kr30F1212 17231 17232 SNP bastl45B0903 17235 17236 SNP Bmac156 — — SSR bah60k06 17239 17240 size_poly (codominant) BaGS32I12 17243 17244 SNP BaGS4H02 17247 17248 CAPS bah44k12 17251 17252 size_poly (codominant) baak27p06 17255 17256 SNP bags21m21 17259 17260 SNP BaSD14J15 17263 17264 CAPS HVM49 — — SSR basd16e11 17267 17268 SNP BaAK21N09 17271 17272 SNP baal0e10 17275 17276 size_poly (codominant) baak1g17 17279 17280 SNP

TABLE 14-9 Primers used Name (SEQ ID NO:) marker type BaH14K12 17283 17284 size_poly (codominant) BaAK31G05 17287 17288 size_poly (codominant) MWG2062 — — STS BaH50G17 17291 17292 size_poly (codominant) HVM5 — — SSR bags38n21 17295 17296 CAPS bastl04B0303 17299 17300 size_poly (codominant) baal35f12 17303 17304 SNP BaH49M02 17307 17308 CAPS baak38f04 17311 17312 CAPS bah29b02 17315 17316 SNP basd15d20 17319 17320 CAPS kr61C0305 17323 17324 CAPS BaH23B08 17327 17328 SNP kr40H1115 17331 17332 CAPS bah51g17 17335 17336 SNP BaSD22E19 17339 17340 SNP

Table 8-1 through Table 14-9 show 7 kinds of polymorphisms: CAPS, SNP, size_poly, SSR (simple sequence repeat), STS, dCAPS, and trait. SSR, STS, dCAPS, and trait are known genetic markers. The polymorphisms specified by the inventors of the present invention are classified into 3 categories: CAPS, SNP, and size_poly. The third category, size_poly, is a polymorphism based on differences in the length of amplified fragments, and it includes polymorphisms that are detected based on whether the primer sets have successfully amplified the fragments. In any case, the polymorphism can be detected by running electrophoresis for the amplified DNA fragments, and detecting differences in band positions, or the presence or absence of bands.

CAPS is a polymorphism based on the presence or absence of a restriction enzyme recognition sequence. Most CAPS are based on single nucleotide polymorphism in the restriction enzyme recognition sequence; however, CAPS also includes those based on insertion/deletion of one or more bases. In Table 8-1 through Table 14-9, the marker type designated as SNP is the marker that does not have single nucleotide polymorphism or insertion/deletion in the restriction enzyme recognition sequence. CAPS can be detected by running electrophoresis for the amplified DNA fragments after restriction enzyme treatment and then detecting the number of bands, or band positions. Non-CAPS SNPs can be detected by typing, i.e., by actually confirming the base at the site of SNP or insertion/deletion. CAPS can also be detected by SNP typing.

Table 15-1 through Table 21-6 show base sequences in the vicinity of SNP or insertion/deletion that occurs between Haruna Nijo and H602. Names of restriction enzymes and recognition sequences of the restriction enzymes are also shown. Tables 15-1 through 15-5 are for 1H chromosome, Tables 16-1 through 16-7 for 2H chromosomes, Tables 17-1 through 17-6 for 3H chromosomes, Tables 18-1 through 18-5 for 4H chromosome, Tables 19-1 through 19-6 for 5H chromosome, Tables 20-1 through 20-5 for 6H chromosome, and Tables 21-1 through 21-6 for 7H chromosome. Table 22-1 through Table 28-25 show base sequences in the vicinity of SNPs of the SNP markers (non-CAPS). Tables 22-1 through 22-20 show polymorphisms in 1H chromosome, Tables 23-1 through 23-30 in 2H chromosome, Tables 24-1 through 24-27 in 3H chromosome, Tables 25-1 through 25-18 in 4H chromosome, Tables 26-1 through 26-28 in 5H chromosome, Tables 27-1 through 27-16 in 6H chromosome, and Tables 28-1 through 28-25 in 7H chromosome. Some of these polymorphisms occur at more than one location in one fragment.

In Table 15-1 through Table 28-25, bases that represent SNP or insertion/deletion are indicated by underline. Note that, in the sequence listing, bases at SNP sites are indicated by “universal codes.” Where applicable, the universal codes are also used for bases in the restriction enzyme recognition sequence.

In Table 15-1 through Table 28-25, bases with SNPs are indicated by N in cases where the base is identified only for one of Haruna Nijo and H602 but not for the other. In this case, the unidentified base is one of the remaining three bases.

Universal codes are defined as follows.

m: A or C

r: G or A

w: A or T

s: G or C

y: T or C

k: G or T

v: A, G or C

h: A, C, or T

d: A, G, or T

b: G, C, or T

n: (A, C, G, or T) or (unidentified other bases)

[Table 15-1] Haruna Nijo SEQ ID Restriction  Recognition Clones H602 NO: enzyme  sequence baak2l03 AGCTTACATCCTGGAATCTTC 17342 MvaI CCWGG AGCTTACATCGTGGAATCTTC BaGS32E23 TGATTGCATCAAAATGGCATG 17353 TaqI TCGA TGATTGCATCGAAATGGCATG basd13k20 CCAGGTGCCGCGGITGTTGCA 17354 HapII CCGG CCAGGTGCCGGGGITGTTGCA baak24o11 GGAAGTTCTCGTCCTTGA-GC 17355 FokI NNNNNNNNNNNNNCATCC GGAANGTCTCATCCTTGAAGG 17356 BaSD2D08 GGGAGCTGCATCAGCAGCCCC 17359 PstI CTGCAG GGGAGCTGCAGCAGCAGCCCC bah11b15 CGGCGCACCCAGCCCTCTCGT 17361 BCnI CCSGG CGGCGCACCCGGCCCTCTCGT BaGS17B21 AGCAGCATGCACATGTGATGG 17375 HhaI GCGC AGCAGCATGCGCATGTGATGG BaAK21D02 CAATGAGGCCATACAGGGCAA 17382 AfaI GTAC CAATGAGGCCGTACAGGGCAA baal4f12 CGAGACCGGCGCTCGATTAAG 17385 HaeIII GGCC CGAGACCGGCCCTCGATTAAG BaGS11I03 GGGCGACCCGGTATGAACAAC 17386 HapII CCGG GGGCGACCCGATATGAACAAC bah19f01 CGAAGTTCCAGGCGTCATTGA 17387 MvaI GCMG CGAAGTTCCAAGCGTCATTGA BaAK27F07 GGAGCGATCGATCGACGGGAC 17388 ClaI ATCGAT GGAGCGATCGGTCGACGGGAC BaH36O18 AGACGAGGGCACTGCTGAGGA 17403 HhaI GCGC AGACGAGGGCGCTGCTGAGGA BaAK20A06 ACAGTAATATCGATTATGAAT ATCGAT ACAGTAATATTGATTATGAAT 17404 ClaI bah25n06 AATCCCTTGACCCAAACAAAG 17405 MboI GATC AATCCCTTGATCCAAACAAAG BaGS11J13 GCACGACACCGGCGTCCTTGC 17413 HapII CCGG GCACGACACCCGCGTCCTTGC baa138123 TGGGTGAGCCTGAGCCGCGAA 17414 DdeI CTNAG TGGGTGAGCCAGAGCCGCGAA BaAL36B15  AGCCTCCATGGCCGCCCATCA 17415 HaeIII GGCC AGCCTCCATGTCCGCCCATCA bast60D0610 CGTCCCAGCTCACAAACTTTG 17416 PvuII CAGCTG CGTCCCAGCTGACAAACTTTG kr22D0808 ATTTGGGGCTGGGTGTCTTTT 17417 XspI CTAG ATTTGGGGCTAGGTGTCTTTT basd23o02 GACCGGATCTGGACGCCAGGA 17422 XspI CTAG GACCGGATCTAGACGCCAGGA baak1j14 ATCTTCTGCAGGCACTTGTCG 17423 PstI CTGCAG ATCTTCTGCAACCACTTGICG baak24k18 CCCCCTGTGGCCACCTCTGGC 17424 HphI NNNNNNNTCACC CCCCCTGTGGTCACCTCTGGC BaH38H09 ATCTTCTGCAAGCACTTGTCG 17426 PstI CTGCAG ATCTTCTGCAGGCACTTGTCG basd17m22 AAGACCCTGTACACCTTGTCC 17450 AfaI GTAC AAGACCCTGTCCACCTTGTCC

[Table 15-2] Harunz Nijo SED ID Restriction   Recognition Clones H602 NO: enzyme sequence bags15g01 TGATCCGAGATATGCAAAGAG 17451 BsmAI NNNNNGAGAC TGATCCGAGACATGCAAAGAG BaH57E12 AATGCCTCGACTTAGTTATGA 17453 DdeI CTNAG AATGCCTCGAATTAGTTATGA baal19m17 ATCTCTCATC-TATCTCGA-A 17461 FokI NNNNNNNNNNNNNCATCC TTCTCTCATCCTATCTCGAAA 17462 bags3p11 ATATGGTCAGTGAAGCCAGGG 17472 TspRI CASTGNN ATATGGTCAGCGAAGCCAGGG bags3p11 TTGAAGGGTCGGTGTTATGGT 17473 TspRI CASTGNN TTGAAGGGTCAGTGTTATGGT BaAK14J21 GCCGCCGCCCGCTGGCAGGAT 17474 TspRI CASTGNN GCCGCCGCCCACTGGCAGGAT baak37g19 AGGAGCAGATGAGGGAGCCGA 17478 MboI GATC AGGAGCAGATGAGGGAGCCGA BaSD16G03 AAAATATTGCTCAGCTTTAAA 17482 HhaI GCGC AAAATATTGCGCAGCTTTAAA BaH13F14 TTTTGTCGTCAACAGATGAAA 17491 TaqI TCG A TTTTGTCGTCGACAGATGAAA basd18l16 CTGCTGAGCACATAGTGGGGC 17496 Bsp1286I GDGCHC CTGCTGAGCACATAGTGGGGC BaAL19J14 AGTCATAGTCAACTACACAAT 17497 TaqI TCGA AGTCATAGTCGACTACACAAT basd17m16 CGATGATGGATGGCTGGAAGA 17507 FokI GGATGNNNNNNNNN CGATGATGGAAGGCTGGAAGA baak13n06 ACCATGTCGCTGGGAAGAGCC 17509 HapII CCGG ACCATGTCGCTGGGAAGAGCC BaGS27M04 CTTGCGTACAGAATCTTGTTG 17511 HinfI GANTC CTTGCGTACAAAATCTTGTTG bags15d20 TATGGACAGTCTAGACAAGCA 17522 XbaI TCTAGA TATGGACAGTTTAGACAAGCA bags37e17 GCTTTGCTTACGAACGGATCT 17563 DdeI CTNAG GCTTTGCTTAGGAACGGATCT BaSD11P04 TAAGGATAATAGACCATTCCA 17569 AvaII GGWCC TAAGGATAATGGACCATTCCA bah12j09 TCTCATGCCGGTATCTTATAG 17570 EcoRV GATATC TCTCATGCCGATATCTTATAG bags14j09 ATGGTATAATTATTCTGTGAG 17573 HinfI GANTC ATGGTATAATGATTCTGTGAG bags14j09 TCGGCGACCGACTCCCAACCA 17574 HinfI GANTC TCGGCGACCGTCTCCCAACCA kr26E0610 GCAAGATGTACCCTGACTTGG 17575 AfaI GTAC GCAAGATGTATCCTGACTTGG BaGS17A18 GGGGGTTGTCACGGCTTTGGT 17579 BstUI CGCG GGGGGTTGTCGCGGCTTTGGT BaSD22O13 GGCAGAGGACCGCCAGAAACG 17581 AvaII GGWCC GGCAGAGGACTGCCAGAAACG bags15a22 TCCAGTGGAGACCCTCGTGGA 17582 HaeIII GGCC TCCAGTGGAGGCCCTCGTGGA baet21H1016 GCTGTTGAGCCACTCCTTTGC 17583 AluI AGCT GCTGTTGAGCTACTCCTTTGC

[Table 15-3] Haruna Nijo SEQ ID Restriction   Recognition Clones H602 NO: enzyme sequence baak34k14 CCGGCACGCCGTCGACCTTCA 17593 SalI GTCGAC CCGGCACGCCATCGACCTTCA bags22f12 CGCCGGATTCCATGCTGCTGC 17595 TaqI TCGA CGCCGGATTCGATGCTGCTGC BaH45P03 CGGCAACGGGATCTTCAGGCC 17596 MboI GATC CGGCAACGGGATCTTCAGGCC basd11d13 CTTGACGCTGAACTCCACGAA 17597 AfaI GTAC CTTGACGCTGTACTCCACGAA bags34j05 CTCAGCCAAGCTCTCCCTAAC 17639 AluI AGCT CTCAGCCAAGTTCTCCCTAAC bah56l03 CTGCTCCTCGAACTCATTGAC 17640 TaqI TCGA CTGCTCCTCGCACTCATTGAC basd12k03 TTCCTAGGACGTTCTTCAAAT 17641 Cfr13I GGNCC TTCCTAGGACCTTCTTCAAAT BaAK39I18 CGGCAACGGGATCTTCAGGCC 17642 MfII RGATCY CGGCAACGGGGTCTTCAGGCC bags21h06 GGAGCTTTTTAAAGCTCCGCG 17643 DraI TTTAAA GGAGCTTTTTGAAGCTCCGCG bah56k04 ACACTAACAAAATTCAGCAAG 17644 HinfI GANTG ACACTAACAAGATTCAGCAAG bah60e11 AGTACAGGTCAAGAAGTTAAA 17645 Cfr13I GGNCC AGTACAGGTCCAGAAGTTAAA baak22i05 TCGGGATAGGCCCAAAACCCA 17646 HaeIII GGCC TCGGGATAGGTCCAAAACCCA baal5i02 TTCATGACCATGGCCUCTGC 17647 NcoI CCATGG TTCATGACCACOGCCCTCTGC BaAK2E05 GCTGCCAACCTAGTACTCTTT 17648 XspI CTAG GCTGCCAACCAAGTACTCTTT baak33g12 CGACCTCGTAGGGCCCGACGA 17649 BanII GRGCYC CGACCTCGTAAGGCCCGACGA bags4e05 ACTAAGTTAGCACATATATCA 17650 AfaI GTAC ACTAAGTTAGTACATATATCA BaH28K13 GGCCGTGGCCGTGAGAGTCGT 17658 Cfr13I GGNCC GGCCGTGGCCCTGAGAGTCGT BaSD1I24 GGCCGTCGAGGGCCATGAACT 17659 Cfr13I GGNCC GGCCGTCGAGAGCCATGAACT bags4e02 TATGAGACTACACTGTGTCCT 17682 TspRI CASTGNN T-TGAGA-TAAACTGG---TT 17683 bast04H0315 CCGTCGACTCGGGCGGATTTA 17684 AvaI CYCGRG CCGTCGACTCCGGCGGATTTA basd2b18 CGAAATATGGCCGCACCTGTG 17688 Hae111 GGCC CGAAATATGGTCGCACCTGTG BaSD14B13 GCCCTGCCCGCCGGCTCCGCA 17700 HapII CCGG GCCCTGCCCGTCGGCTCCGCA BaAK39G10 CAGATGGAGCTGGCGCCTTCA 17724 HapII CCGG CAGATGGAGCCGGCGCCTTCA BaAK39G03 GTACTCCCTCTTGGTGTCGAA 17725 MvaI CCWGG GTACTCCCTCCTGGTGTCGAA baak2a18 TAGACATTACCGGGAGAATCC 17726 HapII CCGG TAGACATTACTGGGAGAATCC

[Table 15-4] Harunz Nijo SEQ ID Restriction   Recognition Clones H602 NO: enzyme sequence basd17l04 CTCCATGGTGCGCCGACGGTG 17730 HhaI GCGC CTCCATGGTGAGCCGACGGTG BaAK30F02 GACTAATAGAGGCCTATATGA 17735 HaeIII GGCC GACTAATAGAAGCCTATATGA bags10j15 GGGTAGTTTCCAAGCTGAAGT 17754 BspT104I TTCGAA GGGTAGTTTCGAAGCTGAAGT bags3c15 ACGGCGTCACATACAGCACGA 17759 AfaI GTAC ACGGCGTCACGTACAGCACGA BaH15M10 TCTCACATTCAAAGATTGTAG 17767 BspT104I TTCGAA TCTCACATTCGAAGATTGTAG bast61E0509 TTTGICTGAGCGCATGAGCTT 17768 HhaI GCGC TTTGICTGAGTGCATGAGCTT BaGS31N04 CAAGTGAGTACACTGACTGTT 17769 TspRI CASTGNN CAAGTGAGTAAACTGACTGTT BaAK27D22 CGATTGACGTTGCACCCTCTG 17770 BanI GGYRCC CGATTGACGTGGCACCCTCTG baal5o19 AGGAATTTTGCATGATAAACT 17771 FokI GGATGNNNNNNNNN AGGAATTTTGGATGATAAACT bah55b18 AT--CCGCAGTGG-ACGCATT 17772 TspRI CASTGNN GTTTCTGCAGGGGCACGCATC 17773 bags15e08 CATTTTGTCTCTTGTTTTCTC 17786 BsmAI GTCTCN CATTTTGTCTTTTGTTTTCTC BaH47J05 TAGTAAAACTCTAAAAAGACT 17814 DraI TTTAAA TAGTAAAACTTTAAAAAGACT baal2j10 TTACATGTCCACTGTGTCTGC 17827 TspRI CASTGNN TTACATGTCCTCTGTGTCTGC BaGS37F14 ACAAACAGCTGCAATTTCCAT 17831 PvuII CAGCTG ACAAACAGCTTCAATTTCCAT BaH21K05 GCCAACAACATTGCACGTACT 17842 Bsp1286I GDGCHC GCCAACAACAGTGCACGTACT BaGS25K24 TTGTTTTGTAGAGCTGCAAAG 17851 AfaI GTAC TTGTTTTGTACAGCTGCAAAG bags1a18 TGGAGTTGGATATCATGCACA 17852 EcoRV GATATC TGGAGTTGGAGATCATGCACA bags35j22 TATTGTTGGGAACCATCTAGT 17866 Cfr13I GGNCC TATTGTTGGGGACCATCTAGT baak20h22 AGTCAACGAGCTCGCCCCACT 17867 SacI GAGCTC AGTCAACGAGATCGCCCCACT bah16j04 CCCTCAGGAAGGCCAATGTGA 17872 HaeIII GGCC CCCTCAGGAAAGCCAATGTGA BaH54J03 ATCTCTCCTCAACTTGTTCAC 17873 TaqI TCGA ATCTCTCCTCGACTTGTTCAC BaH26M05 GTGCTGTCGCAAGCATGACCG 17876 BstUI CGCG GTGCTGTCGCGAGCATGACCG baak14e23 TTAGGGATGCCGGATCAAGTA 17880 HapII CCGG TTAGGGATGCTGGATCAAGTA bastl45e1109 ATCCGAGGATTCACCAGGCTG 17892 MboI GATC ATCCGAGGATCCAGC AGGCTG bah15k16 CCATTGCATTGAACGATTCCA 17902 MseI TTAA CCATTGCATTTAACGATTCCA

[Table 15-5] Haruna Nijo SEQ ID    Restriction Recognition Clones H602 NO: enzyme sequence bags31a22 AAATAACTTGTGICATGTTCA 17903 HinfI GANTC AAATAACTTGAGTCATGTTCA BaGS38N20 GACGGGGTCGGCGATGTGGTC 17917 TaqI TCGA GACGGGGTCGACGATGIGGTC BaGS23O09 AGCCAAACATCGAGAACATGG 17918 TaqI TCGA AGCCAAACATTGAGAACATGG bastl26E0410  AGAGTGCGGGGGGCTCAGTAG 17919 BanII GRGCYC AGAGTOCGGGCGGCTCAGTAG BaH15N14 CTGCGCAATGGCCAACGGTAA 17920 HaeIII GGCC CTGCGCAATGACCAACGGTAA bah30o13 CAAGCCCAGCTCCAAACTACC 17927 AluI AGCT CAAGCCCAGC-CCAAACTACC 17928 bags38f18 GGACAATGGGGATGTTCTCAC 17929 FokI GGATGNNNNNNNNN GGACAATGGGAATGTTCTCAC bah13o05 CTTGTGGACTTGAGTCATCAC 17930 XhoI CTCGAG CTTGTGGACTCGAGTCATCAC baak36b12 GGAGCTCCGGGCGCACGAAGT 17931 BcnI CCSGG GGAGCTCCGGACGCACGAAGT bags18o09 CGTGCTGGCGTTCCAGTAGCC 17932 HhaI GCGC CGTGCTGGCGCTCCAGTAGCC BaAK16L10 CAGCTTAAGCACATTCATAAC 17933 HhaI GCGC CAGCTTAAGCGCATTCATAAC bah13e15 CTTATGTGATCATAATACTGC 17934 MboI GATC CTTATGTGATAATAATACTGC bags1f22 AGCCTTCCACTGGTATCATTT 17935 HapII CCGG AGCCTTCCACCGGTATCATTT BaAL1N23 CGTAGACATGATCGCCATCGC 17945 MboI GATC CGTAGACATGGTCGCCATCGC BaH32B01 ATCACAACTGCGCTACTTTTT 17948 HhaI GCGC ATCACAACTGTGCTACTTTTT BaSD18L13 CCGCTCCTCCATACGATGATG 17949 Afal GTAC CCGCTCCTCCGTACGATGATG BaH58A04 GGAATGACTCGATCAGGTCGC 17950 MboI GATC GGAATGACTCAATCAGGTCGC bah50l05 ATTTTGACTTAGGTCGCACCT 17956 DdeI CTNAG ATTTTGACTTCGGTCGCACCT bastl28a0101  AAAACAGGATGTACACGGCAC  17957 FokI GGATGNNNNNNNNN AAAACAGGATATACACGGCAC

TABLE 16-1 Haruna Nijo SEQ ID    Restriction Recognition Clones H602 NO: enzyme sequence BaAK34H02 ACTTGTGAGACGGTACGTACC 17968 BsmAI NNNNNGAGAC ACTTGTGAGATGGTACGTACC BaGS37P19 CTTCCTCCGATCGATTGCCGC 17971 ClaI ATCGAT CTTCCTCCGACCGATTGCCGC BaAL19P17 TTCCGCTGTAACTCGTGGAGC 17972 AfaI GTAC TTCCGCTGTACCTCGTGGAGC bags38p20 ATTGCTTGAAGGCCTCGAGGT 17983 HaeIII GGCC ATTGCTTGAAAGCCTCGAGGT BaAK41N22 AGTATCCGCCATACGTCCACA 17984 Afal GTAC AGTATCCGCCGTACGTCCACA BaAK21D17 AGGCGTCCTCTAGGGCGCGCT 17985 XspI CTAG AGGCGTCCTCCAGGGCGCGCT bast42A0602 GCACCACCCGCCGGCCGTCGA 17989 HapII CCGG GCACCACCCGTCGGCCGTCGA bast42A0602 GGCAGCAGCCTGTGCGGCACC 17990 HapII CCGG GGCAGCAGCCGGTGCGGCACC BaAK26L07 ACCTTCTACAGGTGCCAAGGC 17995 BanI GGYRCC ACCTTCTACAAGTGCCAAGGC baal12a06 GCAGATATGTTCACATCCCGT 17996 AfaI GTAC GCAGATATGTACACATCCCGT baal12m14 TTCGTCCACTACCTCGATGAA 18003 TspRI CASTGNN TTCGTCCACTGCCTCGATGAA BaH35F01 CGGCGTAGGCGAGCTTGTCGT 18044 HaeIII GGCC CGGCGTAGGCCAGCTTGTCGT BaAL30K02 CAAGGACATCCCTTTGCATTT 18045 FokI NNNNNNNNNNNNNCATCC CAAGGACATCTCTTTGCATTT bags22h22 TACGCAGACAGTGAGATTTTA 18057 TspRI CASTGNN TACGCAGACAATGAGATTTTA bags13n11 CCTGCAGAATCGACAAACTAT 18058 HinfI GANTC CCTGCAGAATGGACAAACTAT bags1h03 GTGGCAAGGCTTGTAGATCTG 18059 HaellE GGCC GTGGCAAGGCCTGTAGATCTG baal19a12 GGTCGCCTACGGGTGAGCATT 18069 HapII CCGG GGTCGCCTACCGGTGAGCATT BaAK45G16 TGCTGAACTCCAGGCAGGGCA 18070 MvaI CCWGG TGCTGAACTCGAGGCAGGGCA BaGS37N19 ACAGCAGGATGCACAATGGAA 18071 MflI RGATCY ACAGCAGGATCCACAATGGAA baak3f03 GAAGGCCTGGAGCCTGGGGGT 18072 HhaI GCGC GAAGGCCTGGCGCCTGGGGGT bah62i11 CTGCGGAGGCTGAACGGCTTG 18081 HaelII GGCC CTGCGGAGGCCGAACGGCTTG bahl7n24 ACAAAGAAGGTCGTTCATGAT 18091 HaeIII GGCC ACAAAGAAGGCCGTTCATGAT BaGS4J04 GGAAACAGCAAATCAAGTACA 18096 MboI GATC GGAAACAGCAGATCAAGTACA baak33f06 ACGTCAAGGGATTCCATTCTT 18097 HinfI GANTC ACGTCAAGGGGTTCCATTCTT

TABLE 16-2 Haruna Nijo SEQ ID Restriction Recognition Clones H602 No: enzyme  sequence basd14f16 GGATGCCCTAGCATAAACTGA   18098 XspI CTAG GGATGCCCTACCATAAACTGA BaH59K20 CTAGCGCCGGCCCGCAGGCAG 18099  HaeIII GGCC CTAGCGCCGGACCGCAGGCAG BaH59K20 TTGTCGGTGGTCTCGTCGCCG 18100  HaeIII GGCC TTGTCGGTGGCCTCGTCGCCG BaGS20M01 CATCAGATATGGAATGATGCA 18107  EcoRV GATATC CATCAGATATCGAATGATGCA bah11n18 TCGGAGCACGGTTCGGCGCTC 18111  HinfI GANTC TCGGAGCACGATTCGGCGCTC baetl8F0911  GCGAGATTTGACCTCACGATA 18113 HaeIII GGCC GCGAGATTTGGCCTCACGATA BaGS4J18 AGTCATAGTCCGCGTCCATGT 18114 BstUI CGCG AGTCATAGTCAGCGTCCATGT baak41d10 GGCAAAATCATTGTAGCAAGG 18127  TspRI CASTGNN GGCAAAATCAGTGTAGCAAGG BaGS14F01 GCAGGTGGTCGACACTGTTAA 18128 TaqI TCGA GCAGGTGGTCAACACTGTTAA BaH52K04 GCAACCAAAATATATTAGCAA 18202 SspI AATATT GCAACCAAAAAATATTAGCAA BaAL37F24   GCAACCAAAATATATTAGCAA 18203 SspI AATATT GCAACCAAAAAATATTAGCAA BaSD11K22 GTTACTTCGGTTCGTCGCCAG 18206  MboI GATC GTTACTTCGGATCGTCGCCAG bags38n06 GATAGGCAACCAGACCAGATT 18213 XspI CTAG GATAGGCAACTAGACCAGATT baet44D1208  AATACAACGCGAATTAATTTA 18214 BstUI CGCG AATACAACGCCAATTAATTTA baal13d11 GCGACGATCCCTGCAGAGCCC 18220  PstI CTGCAG GCGACGATCCTTGCAGAGCCC bah34m23 AAACTTTGTCGGATGCGTCGA 18240  FokI GGATGNNNNNNNNN AAACTTTGTCAGATGCGTCGA BaH50P13 CGACTGCAGCAGCCATGAAGA 18242  AluI AGCT CGACTGCAGCTGCCATGAAGA basd11m16   CTTTCGTGTATAAAGCTGGAG 18243 AfaI GTAC CTTTCGTGTACAAAGCTGGAG bags10p15 AACGAGCATTGAAGATTATTT 18244 MseI TTAA AACGAGCATTAAAGATTATTT bags4g01 GTCGATCAGTGCACGTTTCGC  18245  ApaLI GTGCAC GTCGATCAGTACACGTTTCGC bags10k08 CACATACACTCAATGCCCCGT  18246 MseI TTAA CACATAGACTTAATGCCCCGT bags5e16 ACTTCTTCTCAAGCTCAATCT 18247 BanII GRGCYC ACTTCTTCTCGAGCTCAATCT bags18l02 TGTAGAATACTTCAGTGTCCA 18248  DdeI CTNAG TGTAGAATACCTCAGTGTCCA baak44k02 TGCTTGAACGCGCAGTGAGCC 18249 HhaI GCGC TGCTTGAACGTGCAGTGAGCC

TABLE 16-3 Haruna Nijo SEQ ID Restriction  Recognition  Clones H602 NO: enzyme sequence bags35a20 CTCATTGTTCCGGTTTCACAG 18250 HapII CCGG CTCATTGTTCTGGTTTCACAG BaGS26M11 TAAACAAGTGCGCCCTGAGCC 18251 HhaI GCGC TAAACAAGTGTGCCCTGAGCC BaSD13D12 CGTGCTGCTCCGCGAACCGGA 18259 BstUI CGCG CGTGCTGCTCTGCGAACCGGA BaH42E05 TTCGCATGATTGCATCTACAG 18260 MboI GATC TTCGCATGATCGCATCTACAG bastl55A0701 ACCCCGTATGATACAACAGCG 18261 Afal GTAC ACCCCGTATGGTACAACAGCG kr28B0604 CCGCGTTTCTTGGGGGGTGCT 18265 Aval CYCGRG CCGCGTTTCTCGGGGGGTGCT bah16g18 AAGTGCTACACCCTIGGCTGT 18278 FokI NNNNNNNNNNNNNCATCC AAGTGCTACATCCTTGGCTGT bags35c23 CAACCATCAGCTGGCTACCAA 18284 A luI AGCT CAACCATCAGTTGCCTACCAA BaAK19K05 AAAAGGAGTACTGACTTGATA 18293 Afal GTAC AAAAGGACTATTGACTTGATA bah11i16 CTTGTGCTGGGGACGGCTGAG 18294 HphI GGTGANNNNNNNN CTTGTGCTGGTGACGGCTGAG baak22b17 GAGCTCGACGGGTCCTTTAGC 18325 EcoO109I RGGNCCY GAGCTCGACGAGTCCTTTAGC bags20f22 AACTACTACCNGGTNNGCGAC 18326 BcnI CCSGG AACTACTACCGGGTNNGCGAC baal1d17 CAAACATCCGGAAATGCACAG 18397 HapII CCGG CAAACATCCGAAAATGCACAG bags22f06 ATTAAGAGATCGCCCGAACAA 18398 MboI GATC ATCAAGAGATTGCCCGAACAA bah16a03 TTCCCAGCGGTCGCTGCTGCC 18404 HaeIII GGCC TTCCCAGCGGCCGCTGCTGCC BaAL11F18 AACTTAAGAGATCTACAGCTT 18405 BglIII AGATCT AACTTAAGAGGTCTACAGCTT BaAK29E10  CCGCGTTGCGCTGGAACTCGA 18406 HhaI GCGC CCGCGTTGCGTTGGAACTCGA baak16f14 AAGAAGTAGATCAATCCTCAG 18415 MboI GATC AAGAAGTAGACCAATCCTCAG baak44c12 TATAAGAGCATTTGGGTCACT 18416 Bsp1286I GDGCHC TATAAGAGCACTTGGGTCACT baal39m19 AACTATGAATATTAGACCCTT 18418 SspI AATATT AACTATGAATCTTAGACCCTT baak4e02 TTACAAAATCAAGAAACAAGT 18419 TaqI TCGA TTACAAAATCGAGAAACAAGT baak46f04 TCGATATAGATCAAATAAGAA 18420 MboI GATC TCGATATAGACCAAATAAGAA BaSD19I17 GGTGCGAATCGATGGICCTIC 18425 ClaI ATCGAT GGTGCGAATCCATGGICCTIC BaAK19P01 CAAAAGAGTCTAGTAAATTTG 18427 XspI CTAG CAAAAGAGTCCAGTAAATTTG

TABLE 16-4 Haruna Nijo SEQ ID Restriction   Recognition Clones H602 NO: enzyme sequence BaH26P22 AGAAGCATTGGTCAGATGACC 18429 MboI GATC ACAAGCATTGATCAGATGACC kr59F0311 TTTAGTGAATCCGTATTTTGT 18439 E co RI GAATTC TTTAGTGAATTCGTATTTTGT baal13e10 TTGCTTACAGCTATGGTTCCC 18440 AluI AGCT TTGCTTACAGTTATGGTTCCC BaGS5K11 TCTAGGCCGCCAAGCTCACGA 18442 DdeI CTNAG TCTAGGCCGCTAAGCTCACGA BaGS10J14 TCCCAGCTGACCCAACCATTA 18443 MboI GATC TCCCAGCTGATCCAACCATTA BaGS10J14 ACTCTACAAGATCTTGAA-TT 18444 MboI GATC ACTCTACAAGTTCTTGAAATT 18445 baak41m17 TGATGTGCCGATGAAATCCCT 18446 HapII CCGG TGATGTGCCGGTGAAATCCCT baak41d22 CACGTCTCTAAGACAAGAAAC 18449 XspI CTAG CACGTCTCTAGGACAAGAAAC bah61c16 GGTCGCTCCCAGGTCCITCGC 18450 HapII CCGG GGTCGCTCCCGGGTCCTTCGC BaAL7M13 GGACCGTGTGCCCCTTTGGGT 18451 HaeIII GGCC GGACCGTGTGGCCCTTTGGGT BaAK31F11 GGTCGCTCCCAGGTCCTTCGC 18452 Aval CYCGRG GGTCGCTCCCGGGTCCTTCGC baal33d23 TGGTGGTGACGGCGGCCTGCA 18453 S ac If CCGCGG TGGTGGTGACCGCGGCCIGCA BaH31A03 GCTTGGCATGAATGGCGCACA 18495 FokI GGATGNNNNNNNNN GCTTGGCATGGATGGCGCACA bastl39A0901 CAAAATAAAAGGCTATCAACT 18496 AluI AGCT CAAAATAAAAAGCTATCAACT basd12i15 CTCTAGGCGATAATCACCATC 18509 HinfI GANTC CTCTAGGCGAGAATCACCATC BaAK40I03 CACAATGATTCTATTTTAAAA 18510  H hfI GANTC CACAATGATTTTATTTTAAAA BaAK23E14 GATTCTTCTCAATCTGACGGA 18511 TaqI TOGA GATTCTTCTCGATCTGACGGA baal27a24 TCATCTTGGCAGTTTCACCAC 18513  HaeIII GGCC TCATCTTGGCCGTTTCACCAC BaH28J15 CCTTGGTGTCGAAATGAGACG 18518  TaqI TOGA CCTTGGTGTCAAAATGAGACG baal5b15 CTGGAGACTCAAGCAGATGCA 18526 XhoI CTCGAG CTGGAGACTCGAGCAGATGCA BaH28J10 GCATCGTGCGCGGTGCTCACC 18537 HhaI GCGC GCATCGTGCGTGGTGCTCACC baak34p05 GACACTCAAGTAGTGCGTCGA 18538 TspRI CASTGNN GACACTCAAGCAGTGCGTCGA bah27d18 TCAGTCTGAGTACTAATCAAA 18541 AfaI GTAC TCAGTCTGAG-ACTAATCAAA 18542 BaH50A15 ATAAAAAAAATTCGATCTITA 18546 ClaI ATCGAT ATAAAAAAAAATCGATCTITA

TABLE 16-5 Restriction  Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd2f22 CATGCCTCCACGCCAATCTCC 18548 HaeIII GGCC CATGCCTCCAGGCCAATCTCC BaH47K06 TCCTTCTTTTAAAAAAAAAAA 18549 DraI TTTAAA TCCTTCTTTTCAAAAAAAAAA bags29b01 TTTTTTTAAAAATCCTGGGGG 18555 FokI NNNNNNNNNNNNNCATCC TTCTTTTCAACATCCTCGCGC basd2a21 ACCAAGCAACGCGCATCAACT 18559 HhaI GCGC ACCAAGCAACTCGCATCAACT bags33m15 TACACCTTGTGGATTAATATA 18574 DraIII CACNNNGTG TACACCTTGTCGATTAATATA baak41e24 GCCACTTGTCAATGCAGGCGC 18584 TaqI TCGA GCCACTTGTCGATGCAGGCGC bah63c08 AAGGTCTCATTAAAAAGGTGA 18585 MseI TTAA AAGGTCTCATCAAAAAGGTGA baal17e18 GCTAAGATCCAGAGAAAAGGA 18606 HapII CCGG GCTAAGATCCGGAGAAAAGGA basd11g04 TTTGGATGGTGCCCCAACACT 18608 BanI GGYRCC TTTGGATGGTCCCCCAACACT BaGS36A04 GTTCAGTCTTGAAATGCCCGA 18611 DraI TTTAAA GTTCAGTCTTTAAATGCCCGA bags13a16 GCATCCTTGTCAAGGTACCCT 18612 MseI TTAA GCATCCTTGTTAAGGTACCCT BaAK35F14 TGCACCCTCATGCATCCATAC 18618 DdeI CTNAG TGCACCCTCAGGCATCCATAC baak46l06 GGCCGAGAGGTGTCCGGTAAA 18624 AvaII GGWCC GGCCGAGAGGGGTCCGGTAAA baak15p17 GACTTTGGCCGGGGGATCGCT 18625 HapII CCGG GACTTTGGCCAGGGGATCGCT BaGS33J16 CTATGCTCTGTGCTATAACCA 18626 HhaI GCGC CTATGCTCTGCGCTATAACCA bags7p21 AGAGCAGTGCACACACTTGTG 18627 ApaLI GTGCAC AGAGCAGTGCGCACACTTGTG BaAK22E05 TGAGATAGGGGCCCCAGGAAT 18628 HaeIII GGCC TGAGATAGGGACCCCAGGAAT bah42m05 TGTACTGGTGCTAAGCATGCC 18629 HphI GGTGANNNNNNNN TGTACTGGTGATAAGCATGCC BaAK25L01 CGGCACCGGCGCCGTGTGCCG 18630 HaeII RGCGCY CGGCACCGGCACCGTGTGCCG bags39e24 AAGAAGATGCACGAGGATAGC 18631 BstUI CGCG AAGAAGATGCGCGAGGATAGC bags16d15 GAAGAAACAACTACCCCACTG 18638 AfaI GTAC GAAGAAACAAGTACCCCACTG bah19f21 TTTACAGCCGAGAGATGAGCA 18648 HapII CCGG TTTACAGCCGGGAGATGAGCA bags20bl0 TACCTGCATGTTAACACAAGG 18649 MseI TTAA TACCTGCATGATAACACAAGG BaGS29J10 CCATGGTGGTTCATTTTTGCT 18660 Cfr13I GGNCC CCATGGTGGTCCATTTTTGCT

TABLE 16-6 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd12n12 CCCTGTTCCTGGAAAATGGAA 18675 MvaI CCWGG CCCTGTTCCTAGAAAATGGAA bags6l02 ATTAGGGTATCAATCCTATAC 18677 AseI ATTAAT ATTAGGTCATTAATGCTATAC basd14p15 AAACACAAATATTCCTACAAT 18680 SspI AATATT AGACACAAATCTTCCTACAAT bags19g04 ATCATCCTTCGAACAACATCC 18685 BspT104I TTCGAA ATCATCCTTCAAACAACATCC bah33p11 AGGTAACCAGCTTTAGCCATT 18693 AluI AGCT AGGTAACCAGTTTTAGCCATT bags38f12 TTTACAGTCCGGTAGATTTGG 18704 HapII CCGG TTTACAGTCCAGTAGATTTGG basd26p18 CCTTTGAATTATTTGTAATTT 18705 EcoRI GAATTC CCTTTGAATTCTTTGTAATTT baak45h16 CAGATTGACCGTACCTGGTTA 18706 AfaI GTAC CAGATTGACCATACCTGGTTA bah28p12 AAGGCATGTACGTATGTCATC 18707 Afal GTAC AAGGCATGTAAGTATGTCATC bah19a10 CATGCGCAGCACAGAGAAGTG 18708 AluI AGCT CATGCGCAGCTGAGAGAAGTG basd16l09 CTTCTGAAAGATTACTTTTCG 18717 AluI AGCT CTTCTGAGAGCTTACTTTTCG BaGS18N21 CAATGGCTTCAACAATCCGGA 18742 TaqI TCGA CAATGGCTTCGACAATCCGGA baal12l02 ATTTTATCTAACTAGTCTGTA 18768 AluI AGCT ATTTTATCTAGCTAGTCTGTA bags35a12 GCATGGAACCAGTGTCGGCTT 18775 HapII CCGG GCATGGAACCGGTGTCGGCTT baak42l17 CCCTGTGGCGCGTCTCCTGCT 18785 HhaI GCGC CCCTGTGGCGGGTCTCCTGCT baak36d23 CTTGTCTTCGAGCTGCAAAGA 18786 AluI AGCT CTTGTCTTCGGGCTGCAAAGA bast78C1006 GCACCCAGGGTCGTCTGCCAC 18787 HaeIII GGCC GCACCCAGGGCCGTCTGCCAC BaAK36B07 GGCCATGCAGGATCAGTCCAG 18799 MboI GATC GGCCATGCAGTATCACTCCAG baak26b05 AAGGCTGGCGTCGGCTTGGAC 18800 HapII CCGG AAGGCTGGCGCCGGCTTGGAC bags6n10 ACCACAATTAATACCTGGAGC 18809 AseI ATTAAT ACCACAATTAGTACCTGGAGC bah41b23 TGGCATCCTCAATTGAGTCAA 18820 TagI TCGA TGGCATCCTCGATTGAGTCAA bastl30D0408 GCCTTTTTCGATAAACAAGAA 18822 TaqI TCGA GCCTTTTTCGGTAAACAAGAA bah17p16 CTGGATTTCCGGCTGCCCGAT 18823 HapII CCGG CTGGATTTCCTGCTGCCCGAT basd16e16 TCATTCAAGCTGGCAATAGCA 18835 HapII CCGG TCATTCAAGCCGGCAATAGCA

TABLE 16-7 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bags9p10 CTCACAGAGCGCGGCACGCAG 18838 Bsp1286I GDGCHC CTCACAGAGCACGGCACGCAG basd27d09 AGTTTTTAACTAAGTGCTTCC 18839 DdeI CTNAG AGTTTTTAACAAAGTGCTTCC basd17p09 CCTGTGAAGAGCCCGGATGCT 18844 BanII GRGCYC CCTGTGAAGATCCCGGATGCT bags5m04 AGCCTGCAATTGATATCACTC 18845 ClaI ATCGAT AGCCTGCAATCGATATCACTC BaGS5E06 GGTCATCCGCCGCGCCGTGGT 18846 SacII CCGCGG GGTCATCCGCGGCGCCGTGGT kr49E0610 AGGGATACCATGGAAACAAAA 18850 NcoI CCATGG AGGGATACCACGGAAACAAAA BaGS22E05 AATCATCTGCACTAGCATTTC 18859 HhaI GCGC AATCATCTGCGCTAGCATTTC BaH45O03 CTGCTCAAGCGCTGTGTGAAA 18862 HhaI GCGC CTGCTCAAGCACTGTGTGAAA

TABLE 17-1 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd22i04 TCCTGTACTCAGGTTTCACCA 18870 AvaI CYCGRG TCCTGTACTCGGGTTTCACCA BaGS31M01 ACACGTGCCATGGGTTGGCGG 18871 NcoI CCATGG ACACGTGCCACGGGTTGGCGG basd15n13 AGCTGCCCGGGGAAAAACCCA 18873 AvaI CYCGRG AGCTGCCCGGCGAAAAACCCA bastl04C0406 GACGTGACGGTTCGGGTTGAT 18883 AvaI CYCGRG GACGTGACGGCTCGGGTTGAT baak39l17 CGAGAGCTGGACCATAATGGA 18886 HaeIII GGCC CGAGAGCTGGGCCATAATGGA BaAL17J24 CGACGTACTGATCCTGGCAGA 18887 AvaII GGWCC CGACGTACTGGTCCTGGCAGA bah15p03 GTAAAACCGGATGACAGTTAT 18888 FokI GGATGNNNNNNNNN GTAAAACCGGCTGACAGTTAT baal22cl6 ATAGATAATTCGATATGTGAA 18890 TaqI TCGA ATAGATAATTAGATATGTGAA bags22i13 TGAAGACATCGAGTGATCCCA 18902 TaqI TCGA TGAAGACATCAAGTGATCCCA BaGS20D21 CCACCTTGGCATCCTCCGGCA 18906 FokI NNNNNNNNNNNNNCATCC CCACCTTGGCGTCCTCCGGCA bags39o21 ATCTGCTATAACTATATGAAA 18907 AluI AGCT ATCTGCTATAGCTATATGAAA BaSD19H23 TTCCAGATTCGAAATCATTTC 18914 BspT104I TTCGAA TTCCAGATTCCAAATCATTTC baak35n06 ACGGCACGGTCAGTGTCAGGT 18915 TspRI CASTGNN ACGGCACGGTTAGTGTCAGGT baak35n06 ATTTTAGACATTGTTTGGATC 18916 TspRI CASTGNN ATTTTAGACAGTGTATGGATC bags35b22 AACACTGGTACCTTGTTAGCT 18917 AfaI GTAC AACACTGGTAACTTGTTAGCT BaAK30H06 CTAAACTCGTGGTCCGTCAAC 18918 Cfr13I GGNCC CTAAACTCGTCGTCCGTCAAC BaAL15M07 AACACTGGTACCTTGTTAGCT 18919 KpnI GGTACC AACACTGGTAACTTGTTAGCT BaGS20N02 GATCGCTGGG-GCTATAGGCA 18920 HhaI GCGC GATCGCTGGGCGCTATAGGCA 18921 BaAL19L12 TCTGCACAGCGCCTGGTTGCT 18922 HhaI GCGC TCTGCACAGCACCTGGTTGCT baal1h04 CAGTCGACACTGGCGCTGATC 18923 HapII CCGG CAGTCGACACCGGCGCTGATC bah45n12 GTTGCCCGCGAATCTGCACCG 18933 SacII CCGCGG GTTGCCCGCGGATCTGCACCG baal5k12 CGACCACACGTGTCCCAGCAG 18936 BstUI CGCG CGACCACACGCGTCCCAGCAG baak12j16 AGGTGAACTCGAGGTTTGGGC 18939 XhoI CTCGAG AGGTGAACTCAAGGTTTGGGC BaAK28J20 TCATGGTAAGCTTGCAATGTG 18940 HindIII AAGCTT TCATGGTAAGTTTGCAATGTG

TABLE 17-2   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaH27G14 TCATGGTAAGCTTGCAATGTG 18941 HindIII AAGCTT TCATGGTAAGTTTGCAATGTG baak43h20 TCTGACCATCTTCAGCAGTCT 18947 FokI NNNNNNNNNNNNNCATCC TCTGACCATCCTCAGCAGTCT BaAK30M07 CTGTAGTGCTTCTTCGTCAGG 18951 Bsp1286I GDGCHC CTGTACTGCTCCTTCGTCAGG bags6a04 AATAATTCATCGATTAACAAA 18952 ClaI ATCGAT AATAATTCATTGATTAACAAA baak27d01 TCTAGACGTACTTAGTGCTAC 19001 AfaI GTAC TCTAGACGTATTTAGTGCTAC BaGS27P18 ATGGAGATTTTTAAACTTCCA 19016 Dral TTTAAA ATGGAGATTTATAAACTTCCA BaGS4J14 GGACTTCATGCGCCACTCCAG 19017 HhaI GCGC GGACTTCATGTGCCACTCCAG BaH32J06 ACCAAATGAGTTCATGGAATC 19034 BanII GRGCYC ACCAAATGAGCTCATGGAATC BaH57K23 GTATATGTTTGGCGCCAGGAG 19040 HaeII RGCGCY GTATATGTTTCGCGCCAGGAG baal12d12 TGGCCCAAGATCCCTAGCTGG 19057 MfII RGATCY TGGCCCAAGAACCCTAGCTGG BaSD14G02 ACATGCGAATATTTTTTTAAT 19070 SspI AATATT ACATGCGAATCTTTTTTTAAT BaGS16I18 CAAAAAGAGATGAGGCGAGGC 19081 BsmAI NNNNNGAGAC CAAAAAGAGACGAGGCGAGGC bah11k22 TTATCTTTCCTGGGGTAACCA 19083 BcnI CCSGG TTATCTTTCCGGGGGTAACCA baak32p21 TGTCCCACGCACTGTTGATCG 19084 HhaI GCGC TGTCCCACGCGCTGTTGATCG bah63l21 GAAAAAACTGTGCCTCTACAA 19085 HhaI GCGC GAAAAAACTGCGCCTCTACAA BaGS38D03 CGAGCTTCACTCGGTCACCAT 19086 HapII CCGG CGAGCTTCACCCGGTCACCAT bags23b01 AGTCAAACTCAAGTGAGGAAC 19093 TspRI CASTGNN AGTCAAACTCCAGTGAGGAAC bags29c09 GATGATTGGGGATCTGTTGAT 19094 MfII RGATCY GATGATTGGGCATCTGTTGAT bags6b06 ACAGATTTTGTGCAGCATGTT 19095 HhaI GCGC ACAGATTTTGCGCAGCATGTT BaAK27G06 AGGCCTTGGCAAGCACGTCGT 19096 Bsp1286I GDGCHC AGGCCTTGGCGAGCACGTCGT BaAK39I07 TTGTGCTACCAGGCTTGAATA 19097 BcnI CCSGG TTGTGCTACCGGGCTTGAATA BaGS20G21 AGCTGAGCGCAATTCCTTGTG 19098 HinfI GANTC AGCTGAGCGCGATTCCTTGTG bah11m03 CAGGTGTAGCTCCATATCTGG 19099 AluI AGCT CAGGTGTAGCCCCATATCTGG BaAL3C04 TTCCGGGGATCCTTCCAGCTA 19100 HinfI GANTC TTCCGGGGATTCTTCCAGCTA

TABLE 17-3 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bags24n16 CATGGTGAACCGGGGAGTCTT 19103 HapII CCGG CATGGTGAACAGGTGAGTCTT baal12b04 AATATTCATAGTTAATACACA 19124 AseI ATTAAT AATATTCATAATTAATACACA BaGS36B01 AAAACGATTCACGGTTCAGCT 19131 BstUI CGCG AAAACGATTCGCGGTTCAGCT baal12i18 AGAACTGTGGAGCATACTACT 19150 HhaI GCGC AGAACTGTGGCGCATACTACT BaAL8G07 ACCAGCTGCAGGTTATACCCT 19151 PstI CTGCAG ACCAGCTGCACCTTATACCCT bags3f23 CATTGTGTCTCGAGTCAATGG 19157 XhoI CTCGAG CATTGTGTCTTGAGTCAATGG baal4m06 GGAGAACAAGATCTGCCTCGA 19158 MfII RGATCY GGAGAACAAGGTCTGCCTCGA basd1l23 TTCATTTACTAGATACTCCAT 19177 XspI CTAG TTCATTTACTGGATACTCCAT baak13p20 TATCGTCGGCGTGAATCTGAT 19196 HaeIII GGCC TATCGTCGGCCTGAATCTGAT BaH29I03 TGCAAGGTACCATCCCAGGAT 19211 KpnI GGTACC TGCAAGGTACTATCCCAGGAT bah16h01 CAGCACCGCCAGGAGTTTGCT 19212 MvaI CCWGG CAGCACCGCCGGGAGTTTGCT baal11c20 TTGCTACGAATCCAAGAGAAA 19249 HinfI GANTC TTGCTACGAAACCAAGAGAAA basd15a02 CAGCGGAGCCGGTCATAGGTT 19254 HapII CCGG CAGCGGAGCCAGTCATAGGTT bah15k11 CACAAACGAAAGCGCGTCTTG 19259 HhaI GCGC CACAAACGAANGCGCGTCTTG bah15k11 GCCACGCATGCGCACAAGAAA 19260 HhaI GCGC GCCACGCATGTGCACAAGAAA baal35p14 AGACGACTTCAGCTCTTGATG 19266 AluI AGCT AGACGACTTCTGCTCTTGATG baak11n24 AGTCAAAGGGTTTAGAAACCA 19286 DdeI CTNAG AGTCAAAGGGCTTAGAAACCA BaGS39M09 TGGCAATAAGCATCCCGATGC 19291 HinfI GANTC TGGCAATAAGAATCCCGATGC baak31a24 GTCTTAATAAACTAATGATAG 19307 AluI AGCT GTCTTAATAAGCTAATGATAG baak31a24 TTAAATCTGAGCTTATGAAGA 19308 AluI AGCT TTAAATCTGACCTTATGAAGA baak31a24 TGGAATGCAGCTTCATATGCG 19309 AluI AGCT TGGAATGCAGTTTCATATGCG bags7f08 GGTAAGCGTCGAACAGAGATG 19314 TaqI TCGA GGTAAGCGTCAAACAGAGATG bags38b06 TTGCGACGTTTAAGATATTCG 19318 MseI TTAA TTGCGACGTTGAAGATATTCG bah27a22 AACACCAAGCTTCGTGAGAAT 19330 AluI AGCT AACACCAAGCCTCGTGAGAAT

TABLE 17-4 Restriction  Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAK29G03 CCATGGAGGGCCGATCAGCAG 19335 Cfr13I GGNCC CCATGGAGGGACGATCAGCAG baak23e11 CTGATGATACTAGAGCAGCCA 19340 XspI CTAG CTGATGATACCAGAGCAGCCA bags31k04 TTGTAGAGCTTGCACAATCTT 19341 BanII GRGCYC TTGTAGAGCTCGCACAATCTT BaSD27G02 GCTGCTGTGGACCGAAGGTCG 19350 HaeIII GGCC CCTGCTGTGGGCCGAAGGTCG baak32m10 CCTCGAGGCCAGACTTGAGGC 19352 HapII CCGG CCTCGAGGCCGGACTTGAGGC baak32m10 TGTTGGCCACCCGGATCATGT 19353 HapII CCGG TGTTGGCCACACGGATCATGT BaAL4L02 GCTCGTACTCCGGCGCGGTGT 19355 HapII CCGG GCTCGTACTCTGGCGCGGTGT BaGS32M17 ATACTCCATCCGTCCCGAATT 19375 AvaII GGWCC ATACTCCATCGGTCCCGAATT basd3g08 AGGACTTTGGAATATTATTAA 19377 SspI AATATT AGGACTTTGGTATATTATTAA BaAL7816 CATCAAACATCCATTCCTTCT 19398 FokI NNNNNNNNNNNNNCATCC CATCAAACATACATTCCTTCT baak34g01 TAGATGAAGATACATCCATTT 19406 BsmAI NNNNNGAGAC TAGATGAAGAGACATCCATTT baak12f01 AGAAGGTGGGATGATTATCAG 19407 FokI GGATGNNNNNNNNN AGAAGGTGGGGTGATTATCAG BaGS30I18 CAAATATTTCAATGAAAATAC 19408 TaqI TCGA CAAAT-TTTCGATGAAAATAC 19409 baak40o04 TTGGCGGATTCTTGACGCGGC 19424 HinfI GANTC TTGCCGGATTGTTGACGCGGC bags11i04 GATCATCAACGTACTCAAACT 19432 Afal GTAC GATCATCAACATACTCAAACT bah52o06 AGAATGGCACCGGAGACTAGA 19435 HapII CCGG AGAATGGCACGGGAGACTAGA BaAL25P17 TGGCGAAGTGACAACTGATCT 19441 MboI GATC TGGCGAAGTGGCAACTGATCT BaAL25P17 TGGGTGCTGACCAACGACACA 19442 MboI GATC TGGGTGCTGATCAACGACACA kr24E0709 TACCTTGAATTATCCATCATA 19454 MboI GATC TACCTTGAATGATCCATCATA BaAL13N01 TCTTGGTCGC-CCCGGGGCGA 19458 HhaI GCGC TCTTGGTCGCGCCCGGGGCGA 19459 BaGS31N06 AATGAGTGGTACACGGTCGTG 19460 ApaLI GTGCAC AATGAGTGGTGCACGGTCGTG BaH36L17 GGGTGAACTCAAAAATGCTAA 19490 TaqI TCGA GGGTGAACTCGAAAATGCTAA baak20g24 CCAGGCCATAGCGGGTGCTGT 19494 HapII CCGG CCAGGCCATACCGGGTGCTGT baet46C0206 TGTTGAAGTCTCGGGTGTTGT 19503 BcnI CCSGG TGTTGAAGTCCCGGGTGTTGT

TABLE 17-5   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence baal5i05 TGAGCTTGTCTCGCTTGGACC 19509 HhaI GCGC TGAGCTTGTCGCGCTTGGACC bah14a11 CTGCGTCTATAGCCCCGATGT 19510 HaeIII GGCC CTGCGTCTATGGCCCCGATGT bags31e24 AGGAAGGGCTTTCGACTCGGA 19513 BanII GRGCYC AGGAAGGGCTCTCGACTCGGA basd11a10 AGGCGTCCCCGGCCTGCGCGG 19526 HapII CCGG AGGCGTCCCCAGCCTGCGCGG bah50i12 CGGCGGCGGCACTGTTTGTTG 19527 HhaI GCGC CGGCGGCGGCGCTGTTTGTTG baak45p02 TTTATTACAGTGACTTTCCAA 19530 TspRI CASTGNN TTTATTACAGCGACTTTCCAA bags9l16 TGTTGCAGCAGTCGACGGCAT 19531 SalI GTCGAC TGTTGCAGCACTCGACGGCAT BaSD26O20 TTTCAGTGTCGAACTAAGGGC 19533 TaqI TOGA TTTCAGTGTCCAACTAAGGGC bags5d10 TACCCAGTGAAGCTATAACAA 19534 AluI AGCT TACCCAGTGACGCTATAACAA baal0e07 AGTTTCTTGGCCTGGCAGGGA 19535 MvaI CCWGG AGTTTCTTGGACTGGCAGGGA baak14e02 AAATAGTAAGCTCATGTCCAG 19536 AluI AGCT AAATAGTAAGTTCATGTCCAG bah33f19 GTGCAATTGGNTACAAATGCC 19537 AfaI GTAC GTGCAATTGGGTACAAATGCC baal40i22 GTCATCAGCGGCCCCCCGCAA 19543 HhaI GCGC GTCATCAGCGCCCCCCCGCAA kr69E0810 GATTGTTGATCTATGCAGCAA 19547 MboI GATC GATTGTTGATGTATGCAGCAA BaAK39A15 ACAGTGCCGGCCAGACTGTTC 19568 HaeIII GGCC ACAGTGCCGGACAGACTGTTC basd18o21 TGTCTCTCACTGGGAGCTCCC 19569 BcnI CCSGG TGTCTCTCACCGGGAGCTCCC bags23k14 ACATGTGGACTCCAGATATAA 19575 AvaII GGWCC ACATGTGGACCCCAGATATAA BaGS21H17 GATCACTGGATGACCAACCTC 19576 FokI GGATGNNNNNNNNN GATCACTGGAGGACCAACCTC bah41l03 TGCCGTGGCCGTGCACGATGA 19585 ApaLI GTGCAC TGCCGTGGCCATGCACGATGA kr23D0408 TGTACAGACTCAGCTGAGAGA 19592 HinfI GANTC TGAACAGACTGAGCTGAGAGA baak14i02 ATTACTCAGCTACACACCTAT 19617 AluI AGCT ATTACTCAGCCACACACCTAT bags28c17 TCAACTCCGCGAACGACACTT 19622 BstUI CGCG TCAACTCCGCANACGACACTT bags28c17 TCGATTCCTCGCGCATCTATA 19623 BstUI CGCG TCGATTCCTCCCGCATCTATA basd22g20 TCTTCCCCTCCGGGTACTTCA 19633 HapII CCGG TCTTCCCCTCTGGGTACTTCA

TABLE 17-6  Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaH62H20 CTCAAAAGTTTGAAGGATTGA 19634 TaqI TCGA CTCAAAAGTTCGAAGGATTGA baak23i12 AGCATGCTGAGTTTTGTTTAC 19639 DdeI CTNAG AGCATGCTGATTTTTGITTAC BaAL39F24 CCGTTCTCCGCCGCCATCTCC 19648 HapII CCGG CCGTTCTCCGGCGCCATCTCC BaGS4L04 CTAACAATCTTAAACCCAAAA 19649 MseI TTAA CTAACAATCTCAAACCCAAAA BaGS7G14 TTTATGTGCCTGTAGCGGTTG 19655 HapII CCGG TTTATGTGCCGGTAGCGCTTG basd24i11 CTCTGCACCGGCGGCCTGGCC 19656 AciI CCGC CTCTGCACCGCCGGCCTGGCC

TABLE 18-1  Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAL19O06 TACCAAGCGCGTGTCTAGTGA 19666 BstUI CGCG TACCAAGCGCATGTCTAGTGA baak42c15 TAAATCTCGAGGCCAGTATGA 19675 XhoI CTCGAG TAAATCTCGAAGCCAGTATGA baak40n12 GGAGATATGCTGAGATCCTGC 19676 DdeI CTNAG GGAGATATGCGGAGATCCTGC basd2j05 ACCAACACTCGCCACCATAAG 19677 HphI NNNNNNNTCACC ACCAACACTCACCACCATAAG baak24g04 AACAAGACGCGGCCGTCCTTG 19678 HaeIII GGCC AACAAGACGCTGCCGTCCTTG baal2i22 CGGAAGCAGCACAAAAAAAAA 19684 HhaI GCGC CGGAAGCAGCGCAAAAAAAA- 19685 bags20c22 TTAGTAAGCAATGATGAAGAG 19686 TspRI CASTGNN TTAGTAAGCAGTGATGAAGAG BaH50O22 ATAACTGGACTCAATTCCTTG 19687 HinfI GANTC ATAACTGGACACAATTCCTTG bags35i24 AGAATTTGCCAGTCTCTCCAT 19689 HapII CCGG AGAATTTGCCGGTCTCTCCAT baak37p11 GCTGCCAGCCGATCTGTGCGA 19699 MboI GATC GCTGCCAGCCAATCTG-GCGA 19700 BaH50N14 CTTCAAGGACTTTGCTGTTCC 19712 AvaII GGWCC CTTCAAGGACCTTGCTGTTCC kr70A0202 ACCTGCAGATCAGCCTCGCCG 19713 MboI GATC ACCTGCAGATTAGCCTCGCCG bags20h01 TTAAAATATAAATTCATTGAA 19716 HinfI GANTC TTAAAATATAGATTCATTGAA BaH39P15 GATCAACGGACGATATCGAGA 19717 FokI GGATGNNNNNNNNN GATCAACGGATGATATCGAGA BaAK46O20 TATACCTCATGCGCTTCTCTG 19718 HhaI GCGC TATACCTCATACGCTTCTCTG kr18G0913 GGGCTTAGTTTCGGCCAGGTT 19734 HapII CCGG GGGCTTAGTTCCGGCCAGGTT bagsl5e12 AGCTGACCCGCGCGCATCAGT 19737 AvaI CYCGRG AGCTGACCCGAGCGCATCAGT bags29i09 CGCGCGTGAGGCCCCCCGGCT 19738 HaeIII GGCC CGCGCGTGAGCCCCCCCGGCT baak19m23 GTGCTGAATTTATGTTTCAGC 19739 EcoRI GAATTC GTGCTGAATTCATGTTTCAGC bah11l02 GAATCGCTTCGACAAAAAGTT 19746 TaqI TCGA GAATCGCTTCCACAAAAAGTT bags10l12 GGCTGGATTTTACCTCTCTAA 19750 HphI NNNNNNNTCACC GGCTGGATTTCACCTCTCTAA bags31m13 GCACCCACCTTGGCAGGTCGA 19751 MvaI CCWGG GCACCCACCTGGGCAGGTCGA baal16l11 CATTTTGTAGATTCTTCTATA 19762 HinfI GANTC CATTTTGTAGCTTCTTCTATA BaSD17F09 TGTCAATCTTCATCCTCACCG 19765 FokI NNNNNNNNNNNNNCATCC ACTGATAACTGATCCT-ACAG 19766 BaSD14M08 CGTGCTTGGCGAGCTCGCCGG 19769 BanII GRGCYC CGTGCTTGGCAAGCTCGCCGG

TABLE 18-2   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAL17L08 GTTCAGTCAGTGTTTCTCCCA 19785 TspRI CASTGNN GTTCAGTCAGAGTTTCTCCCA bah61p18 TCAGCTCGGCGGAAGTCAGGT 19786 AciI GCGG TCAGCTCGGCAGAAGTCAGGT bah61p18 TTGTTACCACCGCTGTCTCCG 19787 AciI CCGC TTGTTACCACTGCTGTCTCCG bah63d12 GCGAGGGGCACACGCCGCGGC 19788 Bsp1286I GDGCHC GCGAGGGGCAGACGCCGCGGC BaAK26A03 AAAGAATGAACCAGTTTTTCC 19789 HinfI GANTC AAAGAATGAATCAGTTTTTCC BaAL4D19 TGTTTCTCGGAGCACACCTTG 19797 AvaI CYCGRG TGTTTCTCGGGGCACACCTTG basd13l12 GATATTCCATTGAAGTGAAGA 19804 TaqI TCGA GATATTCCATCGAAGTGAAGA bags11m01 ATAATTTCACTGATCAGAAGT 19805 TspRI CASTGNN ATAATTTCACAGATCAGAAGT basd3d13 GATCGAAGGGCGTATCATATA 19807 HinfI GANTC GATCGAAGGGAGTCTCATATA bags23f08 CTCGTCCATGGTCTGCATCGG 19809 NcoI CCATGG CTCGTCCATGCTCTGCATCGG BaGS15L23 CTGCAC-ACTCGATTGCTTAA 19810 TaqI TCGA CTGCACGACTTGACTGCTTGA 19811 BaGS15L23 TACAGTTGTCGAACAATTACA 19812 TaqI TCGA TACAGTTGTCAAACAATTACA baak2k13 ACCCACGAGTACCGTCTGGCA 19813 AfaI GTAC ACCCACGAGTGCCGTCTGGCA baak44g22 TTGTCAACTATGCGAGGATAT 19817 BstUI CGCG TTGTCAACTACGCGAGGATAT basd15p13 CTATGTCTAATTAATGTCTAA 19829 AseI ATTAAT CTATGTCTAACTAATGTCTAA bags21k10 CTAAATCAGTTGACAGCAAAA 19832 TaqI TCGA CTAAATCAGTCGACAGCAAAA bah56n18 TGATGTAGTCAACGATCTCGT 19833 TaqI TCGA TGATGTAGTCGACGATCTCGT baak33k19 GGACGTACGGTTAGACCTCGA 19856 XspI CTAG GGACGTACGGCTAGACCTCGA bah50g09 GCCGCCCGCCCCGATTTGTTT 19858 TaqI TOGA GCCGCCCGCCTCGATTTGTTT bah54l11 CAAAACGCAGGTCCTCAGCCC 19860 AvaII GGWCC CAAAACGCAGATCCTCAGCCC basd13i14 TCGGCGGGATCACTTGGCCAT 19861 MboI GATC TCGGCGGGATTACTTGGCCAT bah56g09 ATAGGAGAGTCCGGCCTGGAG 19862 HinfI GANTC ATAGGAGAGTTCGGCCTGGAG bah41b06 GCCCCTCCCTCGAGTTGACGA 19863 MvaI CCWGG GCCCCTCCCTGGAGTTGACGA bags14n08 TGAACGATCCGGATAAAGAAC 19880 HapII CCGG TGAACGATCCAGATAAAGAAC BaAK36I12 TTAAGTGGACGAGTGATTGCA 19882 XspI CTAG TTAAGTGGACTAGTGATTGCA

TABLE 18-3   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaGS7M13 ATGATCCTGAGGCTGAACATG 19908 DdeI CTNAG ATGATCCTGATGCTGAACATG BaAK32D20 TCCTTGGGTACTATATCAGGT 19914 AfaI GTAC TCCTTGGGTATTATATCAGGT baak46m13 ATGCCCGGAAACTGGGATGTT 19916 HinfI GANTC ATGCCCGGAATCTGGGATGTT bags35n16 GGAAAATTCTCGATTCATAAA 19917 TaqI TCGA GGAAAATTCTTGATTCATAAA baal33a06 CCGCGGCCTGAGCGGTTTGCG 19920 DdeI CTNAG CCGCGGCCTGTGCGGTTTGCG BaSD17F20 AGAGCAACACGATCATCTATT 19921 MboI GATC AGAGCAACACAATCATCTATT bags22i21 AGTTGTGCGCATTGAAACAAA 19922 BstUI CGCG AGTTGTGCGCGTTGAAACAAA BaAL37O23 GGACGAGCCGAGCATCGACTC 19925 BcnI CCSGG GGACGAGCCGGGCATCGACTC bags22p03 CTGCCTTGCGTATCAAGGGGA 19926 MboI GATC CTGCCTTGCGGATCAAGGGGA kr65H0816 CCATGATGGACCTATATACAA 19946 Cfr13I GGNCC CCATGATGGATCTATATACAA bags37j11 ATCGCCCTCCGGATTCCTTTT 19956 HapII CCGG ATCGCCCTCCTGATTCCTTTT bags37j11 TTTGGTCTACAGGGTTACCGC 19957 HapII CCGG TTTGGTCTACCGGGTTACCGC BaAK46L15 GATTATAGTACAGCGGCGACC 19958 AfaI GTAC GATTATAGTATAGCGGCGACC bags1l16 AGTGCACTGGCGCTAAGTATG 19967 HhaI GCGC AGTGCACTGGTGCTAAGTATG basd1d10 AGAGATTTCGGGACGTACCAG 19998 BstUI CGCG AGAGATTTCGCGACGTACCAG baak33j06 GGGCTTGAGCGCAAGTGACAG 20002 HhaI GCGC GGGCTTGAGCACAAGTGACAG bags23a11 CCCAGTAAAGCATGTTGGAAG 20003 FokI GGATGNNNNNNNNN CCCAGTAAAGGATGTTGGAAG bags34f02 TACGACTCGAGCATTGGGCCT 20004 XhoI CTCGAG TACGACTCGAACATTGGGCCT baal20a14 TAGCACAAATATTCAAGAATC 20009 SspI AATATT TAGCACAAATGTTCAAGAATC BaGS21B04 ACCCTGTATTAAGAAGAGTAA 20010 MseI TTAA ACCCTGTATTCAGAAGAGTAA basd11p10 ATTAGAATTCCAGTACACAAG 20013 XspI CTAG ATTAGAATTCTAGTACACAAG BaG333El7 CTGGGGCGCCCGAGCCGACGC 20014 AvaI CYCGRG CTGGGGCGCCGGAGCCGACGC BaGS17A15 AGTGGGCAGCCCTGATCTCAG 20029 AluI AGCT AGTGGGCAGCTCTGATCTCAG baal17m22 TCAGGTTGGAGCCATGGAGGT 20031 MboI GATC TCAGGTTGGATCCATGGAGGT baak42f04 AGTGGGCAGCCCTGATCTCAG 20032 AlUI AGCT AGTGGGCAGCTCTGATCTCAG

TABLE 18-4   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAK2I20 CCGGAGGGACGCCCCACAGCA 20045 HinfI GANTC CCGGAGGGACTCCCCACAGCA basd11l18 TCAGGACTCAAGACCACATAG 20050 AvaII GGWCC TCAGGACTCAGGACCATATAG BaSD13H20 CTCCGGGCAAGAATCTTCAGG 20057 HinfI GANTC CTCCGGGCAACAATCTTCAGG BaSD14O04 TTGGCGGCCTGGCCGGCGAGC 20058 MvaI CCWGG TTGGCGGCCTCGCCGGCGAGC BaGS9H13 ATACTCTGGTGGAACTGGAGG 20071 TaqI TCGA ATACTCTGGTCGAACTGGAGG baal33e04 GAAGACATGTCAACCACACCA 20072 MseI TTAA GAAGACATGTTAACCACACCA BaAL40L16 TATACTCCGGATCTTCATGCT 20073 MboI GATC TATACTCCGGTTCTTCATGCT bags9k13 AGGAGCAGGCACGAGAACGTT 20079 HhaI GCGC AGGAGCAGGCGCGAGAACGTT bags8a14 CTGCGAGCTCCGAAGATGTTG 20081 DdeI CTNAG CTGCGAGCTCAGAAGATGTTG BaH33B15 TGCACCCATTCGACACCATGT 20084 TaqI TCGA TGCACCCATTTGACACCATGT BaSD23P08 TCGTCATGTTGGCCCTCTGCA 20085 HaeIII GGCC TCGTCATGTTAGCCCTCTGCA bast79G0313 CGTTCTGCGGTGCCTCGGCCA 20088 HhaI GCGC CGTTCTGCGGCGCCTCGGCCA baal4o09 TGCCTCTGAATCTCTGCTTGC 20091 HinfI GANTC TGCCTCTGAACCTCTGCTTGC bast40C0206 CTCCACTCCCGCGCCTTCTTC 20117 BstUI CGCG CTCCACTCCCTCGCCTTCTTC baal40g05 GGAACGTGTCGAATTCCTCAT 20118 EcoRI GAATTC GGAACGTGTCAAATTCCTCAT BaAK12L24 TGAACCCACCAGGTTCGCAGC 20121 MvaI CCWGG TGAACCCACCGGGTTCGCAGC BaAL12F24 GAAATCCTCGATCGGTCCTGG 20122 MboI GATC GAAATCCTCGGTCGGTCCTGG BaGS32G16 ACTCGTCGCCAGCGAGGTACT 20138 HapII CCGG ACTCGTCGCCGGCGAGGTACT basd19p22 CTTTACCCACGACTCGAGCGT 20157 HinfI GANTC CTTTACCCACAACTCGAGCGT bags4e03 TTTCATCTACGTACTACCTGC 20158 AfaI GTAC TTTCATCTACTTACTACCTGC BaAK36A13 ATCCTCACGCGAGCTTGCAAG 20159 HindIII AAGCTT ATCCTCACGCAAGCTTGCAAG bags33i03 ATTTGAGTGCTCAGCCTATAT 20160 HhaI GCGC ATTTGAGTGCGCAGCCTATAT BaAK14F03 ATATATGAAACTGAGACGACC 20161 DdeI CTNAG ATATATGAAATTGAGACCACC bags39m22 CGGACGATATCAGCTAGCTAG 20180 EcoRV GATATC CGGACGATATGAGCTAGCTAG baak29a01 CCTCACCGGCCCTTGCAACGG 20183 Cfr13I GGNCC CCTCACCGGCGCTTGCAACGG

TABLE 18-5   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd1e04 GAAAACATGCCGGTGAACTCA 20184 HapII CCGG GAAAACATGCAGGTGAACTCA BaGS23K09 TGGCGGCCGCGTCGCCTTTCA 20185 BstUI CGCG TGGCGGCCGCATCGCCTTTCA BaH42C12 TCACAAAACATTAAACCCCCA 20187 MseI TTAA TCACAAAACAATAAACCCCCA baak15k23 GTCACCCAAATCATGGTCATT 20190 NcoI CCATGG GTCACCCAAACCATGGTCATT bags3h19 GCGGGCGCCGTGGGTCGAGCG 20196 SacII CCGCGG GCGGGCGCCGCGGGTCGAGCG bah17h20 GCACCTTGGGAGCCTTACCTT 20199 HaeIII GGCC GCACCTTGGGGGCCTTACCTT BaAL5L13 TTGAGATCGTGTACTCGAGAA 20200 AfaI GTAC TTGAGATCGTATACTCGAGAA bastl41C0806 GCGTGCGGGCGCCCTTCTGGC 20207 HhaI GCGC GCGTGCGGCCACCCTTCTGGC BaAL5E04 ATTCAGTTGGTGCAGTTCTTA 20216 HhaI GCGC ATTCACTTGGCGCAGTTCTTA baal39f20 ACCGGCACCATGGCTAAGATC 20217 NcoI CCATGG ACCGGCACCACGGCTAAGATC baak43d13 CAGTCTCCCCAGCAATTGCAG 20218 HapII CCGG CAGTCTCCCCGGCAATTGCAG baet23B0604 GCTCCCGCCGGCAGCAGGTTC 20221 HapII CCGG GCTCCCGCCGCCAGCAGGTTC BaAK28I08 ACAATGAGTTCGAAAACACGT 20222 BspT104I TTCGAA ACAATGAGTTTGAAAACACGT BaGS17E03 AAAGAACACAATGGTTGTTTT 20229 TspRI CASTGNN AAAGAACACACTGGTTGTTTT

TABLE 19-1   Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAL21J19 GTGTCGACGCAGAACTCGCCG 20232 BstUI CGCG GTGTCGACGCGGAACTCGCCG BaGS11K08 CCTTGAATCACTGAATTTCCT 20236 TspRI CASTGNN CCTTGAATCATTGAATTTCCT BaGS11K08 TACTCCTACAGTGCTGCCAAG 20237 TspRI CASTGNN TACTCCTACAATGCTGCCAAG BaGS39H16 CATTGTCCGCCGGGCGTTGCT 20238 HapIII CCGG CATTGTCCGCTGGGCGTTGCT BaAK28L16 AGAAGGTTTTTGCCACCGAAG 20246 HaeIII GGCC AGAAGGTTTTGGCCACCGAAG bags35o06 GAGGGAAGTCAAAAAATAGGA 20247 TaqI TCGA GAGGGAAGTCGAAAAATAGGA bags13d07 TTCTGTTTGTGCTCCTGCTGC 20248 Bsp1286I GDGCHC TTCTGTTTGTCCTCCTGCTGC BaAK18A05 TTTGCGCGCTTGAGGAGTCTC 20257 TaqI TCGA TTTGCGCGCTCGAGGAGTCTC BaH38D03 TTTGCGCGCTTGAGGAGTCTC 20258 XhoI CTCGAG TTTGCGCGCTCGAGGAGTCTC BaH47G19 TGGCGGCCTCGACCATGTTGG 20272 TaqI TCGA TGGCGGCCTCAACCATGTTGG BaAK38H10 TCTAATGAATGGCCGCGACGA 20273 HaeIII GGCC TCTAATGAATCGCCGCGACGA BaH31H16 GTGGTCTGTCAAGTCTCAAAT 20278 HinfI GANTC GTGGTCTGTCGAGTCTCAAAT bags16g24 GATGATGGACCCCCGGGCGGT 20293 AvaII GGWCC GATGATGGACACCCGGGCGGT bags35i06 TCTTGGGGCATTAAAATATAT 20298 MseI TTAA TCTTGGGGCAATAAAATATAT bah56c06 TCCTAGCCTCGAGGAGTGAAT 20303 XhoI CTCGAG TCCTAGCCTCAAGGAGTGAAT bags6j06 GTGGGTATGCGCTTCCCAGCT 20305 HhaI GCGC GTGGGTATGCACTTCCCAGCT baak30k04 AAGTGCTCCATCCTGGTCTTT 20307 FokI NNNNNNNNNNNNNCATCC AAGTGCTCCAGCCTGGTCTTT bags39m02 AGACGTCGCCGTCGATGAAGG 20309 ClaI ATCGAT AGACGTCGCCATCGATGAAGG baak36b16 ACACGTAGTAATGCACGTCAA 20315 ApaLI GTGCAC ACACGTAGTAGTGCACGTCAA bah28l03 CACGTACCAGTTCATTAGCAC 20392 AluI AGCT CACGTACCAGCTCATTAGCAC BaAK36B17 ACTTACAATCTGCGACATAAA 20393 BstUI CGCG ACTTACAATCGGCGACATAAA bags15i11 CGGTGGCCGCACTCCCTGTAC 20414 HhaI GCGC CGGTGGCCGCGCTCCCTGTAC BaSD20M22 TCACCACCTTTTGAAAATGCT 20415 DraIII CACNNNGTG TCACCACCTTGTGAAAATGCT bags7a20 TATGAACTGCACCATCTAGTG 20418 HhaI GCGC TATGAACTGCGCCATCTAGTG bah25l12 CTGCTGATTTCACCCAATCCC 20419 HphI NNNNNNNTCACC CTGCTGATTTGACCCAATCCC

TABLE 19-2  Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bags6k09 TAACCACCGTACCTGTGGGCC 20420 AfaI GTAC TAACCACCGTGCCTGTGGGCC BaGS29P21 AGCATATATTCGAAAGATGAC 20421 BspT104I TTCGAA AGCATATATTTGAAAGATGAC bah58f18 TGCACCGGCGCCACCGACAAC 20422 HhaI GCGC TGCACCGGCGGCACCGACAAC bags39i18 CGGGAATTTTCAATTTTATAA 20423 DraI TTTAAA CGGGAATTTTAAATTTTATAA kr07D0208 CCTTGTCATGGTCCTGAATTC 20430 MboI GATC CCTTGTCATGATCCTGAATTC BaGS38M11 CAAAGACATCTGGACGAACCA 20432 HapII CCGG CAAAGACATCCGGACGAACCA bags3k24 GGTGCCCGCGACAAATGCAAA 20433 SacII CCGCGG GGTGCCCGCGGCAAATGCAAA bah53e16 CACAAGCTGAGGCTACTAAAA 20434 DdeI CTNAG CACAAGCTGAAGCTACTAAAA bags21n10 CCCATGACCGATACGACTGGG 20435 HapII CCGG CCCATGACCGGTACGACTGGG bags21n10 TGGTGCTCGCCGGGCGGTAGT 20436 HapII CCGG TGGTGCTCGCNGGGCGGTAGT BaGS25E06 ACAGATGGGGACGCAATGCAA 20437 HhaI GCGC ACAGATGGGGGCGCAATGCAA bah38n03 CTCTGTAGGGGTGCTGTGGAG 20438 FokI GGATGNNNNNNNNN CTCTGTAGGGATGCTGTGGAG BaAK38G14 GGTGCCCGCGACAAATGCAAA 20439 SacII CCGCGG GGTGCCCGCGGCAAATGCAAA baa5a06 CAACAGGGAGCAGCTTTTGGC 20440 HindIII AAGCTT CAACAGGGAGAAGCTTTTGGC basd26d19 AAGAATACTGGACCTCGAGAC 20441 Cfr131 GGNCC AAGAATACTGCACCTCGAGAC BaH50G14 GCAACAAGATCGAGAAATTTG 20443 MboI GATC GCAACAAGATTGAGAAATTTG BaGS38J23 GAGCGCGCTCGATATGCTTAG 20455 TagI TCGA GAGCGCGCTCAATATGCTTAG BaH33A16 ATGCAACTCTAAACACAAAAT 20457 MseI TTAA ATGCAACTCTTAACACAAAAT bags10e03 ATAATCAGTCAACCAAAATTG 20458 TaqI TCGA ATAATCAGTCGACCAAAATTG bags14f17 GAAGCAGGCGCTTCGACAAAA 20459 HhaI GCGC GAAGCAGGCGTTTCGACAAAA baak1e17 CTGCCCCATCAACCAAGCACT 20485 TaqI TCGA CTGCCCCATCGACCAAGCACT baak35j18 CTTGGCACCAATGTGACCTGT 20495 TspRI CASTGNN CTTGGCACCAGTGTGACCTGT bags14h08 TCATCAACTAGCTAAGCTACG 20508 XspI CTAG TCATCAACTAACTAAGCTACG BaSD15J20 ATCAACCGTGATCTCCAGGTG 20532 MboI GATC ATCAACCGTGCTCTCCAGGTG bah39b06 AACATTCTCCAGGACAATCCT 20533 BcnI CCSGG AACATTCTCCGGGACAATCCT

TABLE 19-3 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bags19j08 AGGGAGGGCTCGGTAGCTGTG 20534 BanII GRGCYC AGGGAGGGCTTGGTAGCTGTG BaGS1009 ACCGCATGCGCGTCTACGTCT 20535 HhaI GCGC ACCGCATGCGTGTCTACGTCT bah63j06 GGTGCATAACATCCATCTCGA 20536 FokI NNNNNNNNNNNNNCATCC GGTGCATAACTTCCATCTCGA baa5j24 CATGAGTGCGCTGTGTCCTGA 20542 HhaI GCGC CATGAGTGCGTTGTGTCCTGA bah15h18 TCATCGCCACAAATCACATGA 20543 HinfI GANTC TCATCGCCACGAATCACATGA bast62E0610 CATGAGTGCGTTGTGTCCTGA 20544 HhaI GCGC CATGAGTGCGCTGTGTCCTGA bast62E0610 TCANGACACAACGCACCNNAG 20545 HhaI GCGC NCANGACACAGCGCANNGGGG baak21h06 TCCAGTTGCGGTTGAGGGTGG 20562 HhaI GCGC TCCAGTTGCGCTTGAGGGTGG bags3b05 AGAAAAGAGTCGACAGATTAT 20563 HinfI GANTC AGAAAAGAGTTGACAGATTAT baak16a11 CTCTGTGAACCGGGCTCCTAA 20569 HapII CCGG CTCTGTGAACTGGGCTCCTAA baak15n22 CGCCTTTGGCTCCCTTGAGAG 20570 HhaI GCGC CGCCTTTGGCGCCCTTGAGAG baak38d20 AGGCTCAAACACGAATTTTAA 20578 BstUI CGCG AGGCTCAAACGCGAATTTTAA BaSD12L21 GGCCGCACGGTGCCGAGAACC 20579 HhaI GCGC GGCCGCACGGCGCCGAGAACC BaAK28L22 TGTAATTGTACACAAGATTGG 20582 AfaI GTPC TGTAATTGTAGACAAGATTGG bags21105 CTTCACCGGGTCTCAGTGCAC 20590 Cfr13I GGNCC CTTCACCGGGCCTCAGTGCAC basd15e02 ATTTGGGTGTTGAAAGCTCAG 20594 TaqI TCGA ATTTGGGTGTCGAAAGCTCAG bah11m18 TGGCACTATTTATCTCTCAGG 20597 AseI ATTAAT TGGCACTATTAATCTCTCAGG BaAK30H10 CACAGATGAAGAATCGTTCTT 20608 HinfI GANTC CACAGATGAATAATCGTTCTT bags38b16 ATTCTCGGTGTGCCGAAGCTT 20609 HhaI GCGC ATTCTCGGTGCGCCGAAGCTT bags22f10 TACGACGACGGGCAGCCTTGG 20614 BstUI CGCG TACGACGACGCGCAGCCTTGG BaAK29B22 CCTACTCGGCTATGCAGGGGT 20628 HaeIII GGCC CCTACTCGGCCATGCAGGGGT BaAK27C16 CCATACGTGTAAATTCGAATG 20629 EcoRI GAATTC CCATACGTGTGAATTCGAATG BaGS22H13 CCTGGCTATACTACTCATCTT 20630 AfaI GTAC CCTGGCTATAGTACTCATCTT BaAK27E07 CTACATGGGCTAGCCAAGAAG 20638 Cfr13I GGNCC CTACATGGGCCAGCCAAGAAG bags28o18 GGCATCTTCGTGGGGGAGTTC 20640 BstUI CGCG GGCATCTTCGCGGGGGAGTTC

TABLE 19-4 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence baal25b05 CCGCAGAACTCCGGACGTAGA 20641 HapII CCGG CCGCAGAACTGCGGACGTAGA bags37d20 CAGGTGAGTACAGAAGCACCA 20645 AfaI GTAC CAGGTGAGTAAAGAAGCACCA basd2a02 CCTTGATGTCCACTCCGGTCG 20652 HinfI GANTC CCTTGATGTCGACTCCGGTCG BaGS31L06 TGCTTATGTCAAAGTACTTGG 20653 TaqI TCGA TGCTTATGTCGAAGTACTTCG BaAK19H14 TGATTGATTCAGCTGAAAGTA 20661 PvuII CAGCTG TGATTGATTCGGCTGAAAGTA BaH49L06 TTAGGATCGACGAGCACCGGG 20667 ClaI ATCGAT TTAGGATCGATGAGCACCGGG baak4j01 TGTTGGCTTGTTGCAGCATAG 20688 PstI CTGCAG TGTTGGCTTGCTGCAGCATAG BaAK27E01 ATACCATGTCTATTTGGAAGC 20689 TaqI TCGA ATACCATGTCGATTTGGAAGC bags19p05 TGTCACATTCATAGCTTGTAC 20691 AfaI GTAC TGTCACATTCGTAGCTTGTAC bags35i04 TCCCCATAAGCATCATCCTCC 20698 HinfI GANTC TCCCCATAAGAATCATCCTCC baal19m08 TCAAGTGGATGAATAACTGGG 20703 FokI GGATGNNNNNNNNN TCAAGTGGATAAATAACTGGG baak12d06 CACCAAATCGACGCAGCAAGG 20711 HhaI GCGC CACCAAATCGGCGCAGCAAGG basd14b04 CATAACCATTAGAAATGTTTC 20722 BspT104I TTCGAA CATAACCATTCGAAATGTTTC bags33j12 CCAGAGGGAGCTCCTATACCC 20745 BanII GRGCYC CCAGAGGGAGTTCCTATACCC BaGS30N07 TGAGCACGCCGCGGGTCTCCT 20746 BcnI CCSGG TGAGCACGCCCCGGGTCTCCT BaH37I18 TTCTGAATGGCCCTTTGACTA 20747 HaeIII GGCC TTCTGAATGGTCCTTTGACTA baak46p24 TTCTGAATGGCCCTTTGACTA 20748 HaeIII GGCC TTCTGAATGGTCCTTTGACTA BaGS20F10 GACGGTTAACTCAGCAAAGGA 20757 DdeI CTNAG GACGGTTAACCCAGCAAAGGA bah33p03 TTTATCCCAGCTGGTGCGCAT 20774 PvUII CAGCTG TTTATCCCAGTTGGTGCGCAT bags25h01 AATCCACATCAAGGATGCCTC 20789 XspI CTAG AATCCACATCTAGGATGCCTC baak33m08 TTCTTTTAAGTTAAAAGTGGG 20790 AluI AGCT TTCTTTTAAGCTAAAAGTGGG BaH47A11 GAACGGCCTCAAGAGCCTTGC 20793 XhoI CTCGAG GAACGGCCTCGAGAGCCTTGC bags39e22 CGAATGGCTTTAACGACCTTA 20810 MseI TTAA CGAATGGCTTCAACGACCTTA bags19i06 CCTAGTAGTACGCAAAAACAC 20815 AfaI GTAC CCTAGTAGTATGCAAAAACAC basd11k21 AACGGGGCGCAAGAGTGAAGT 20819 BstUI CGCG AACGGGGCGCGAGAGTGAAGT

TABLE 19-5 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd13j03 AATCCTGGAGTCGACTGATGT 20840 SaII GTCGAC AATCCTGGAGGCGACTGATGT BaSD18I03 GACAGATTTATAAGAAGTTTT 20842 DraI TTTAAA GACAGATTTAAAAGAAGTTTT bast34D0608 GTAACACAGAACGAGTACCCC 20844 MboI GATC GTAACACAGATCGAGTACCCC BaGS30F06 CATCGGTGTTTGACTTACCAT 20845 TaqI TCGA CATCGGTGTTCGACTTACCAT bags21k16 GAATTGTTTCAGCCTTCTTTT 20846 HaeIII GGCC GAATTGTTTCGGCCTTCTTTT BaH48GC1 CCAGGTTCGGCGCCGACCCGG 20847 HaeIII GGCC CCAGGTTCGGGGCCGACCCGG bags39a24 AGAGAACATCGGTGATGCTCT 20848 TspRI CASTGNN AGAGAACATCAGTGATGCTCT bagsl7p10 CATCAGGCATGTGCAGATAGT 20851 PstI CTGCAG CATCAGGCATCTGCAGATAGT BaH57L13 TCGGACGGTAATACTTGTTGG 20852 AfaI GTAC TCGGACGGTAGTACTTGTTGG bags4p07 CTTCTTTTGCGCTGCATCTCA 20853 HhaI GCGC CTTCTTTTGCACTGCATCTCA bah63a08 ACTTCAAGTGCAATCGTGCTA 20854 HinfI GANTC ACTTCAAGTGGAATCGTGCTA bah56j15 CGCCCTTGATGACACCAACAG 20855 MboI GATC CGCCCTTGATCACACCAACAG BaH50F21 TGTGGAAGTTTGAGACCACCA 20857 TaqI TCGA TGTGGAAGTTCGAGACCACCA bags34e15 TCCATCGGTCAAGCATTCCTG 20868 TaqI TCGA TCCATCGGTCGAGCATTCCTG bah13b13 CAGTTGGACTTGCGCAGAAAT 20869 HinfI GANTC CAGTTGGACTCGCGCAGAAAT bah26p09 TATAGATAGCTGAACAATTGC 20889 AluI AGCT TATAGATAGCCGAACAATTGC BaH41C21 CATTGTCTTCCGCAGCAGATT 20897 PstI CTGCAG CATTGTCTTCTGCAGCAGATT basd19g21 ATCCTGTTGCACCCGCCTTCT 20898 HhaI GCGC ATCCTGTTGCGCCCGCCTTCT BaGS36M20 AGGCATAAGATATCGCTCACA 20905 EcoRV GATATC AGGCATAAGAAATCGCTCACA bags21m10 ATTTGCTCGGCGCTCTTTTTG 20906 Bsp1286I GDGCHC ATTTGCTCGGTGCTCTTTTTG BaAK14G11 TACATTCGTTCAAAAATTCTT 20907 DraI TTTAAA TACATTCGTTTAAAAATTCTT BaGS30L20 ATACAAGACCCGAGATAAACC 20908 AvaI CYCGRG ATACAAGACCTGAGATAAACC bah53j08 AGCGTTTAGACCGGGTTGCCG 20923 BcnI CCSGG AGCGTTTAGATCGGGTTGCCG bags21d10 AACAAGAATAGATTCAACCTG 20924 HinfI GANTC AACAAGAATACATTCAACCTG bags9a01 CGAGCCCGGCGTACTGCACGA 20925 AfaI GTAC CGAGCCCGGCATACTGCACGA

TABLE 19-6 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bags34d17 AACTAATCAGCTTAACAATTG 20933 AluI AGCT AACTAATCAGATTAACAATTG baal40k24 AAGTTCCCATTTGAGTGTCTG 20934 DdeI CTNAG AAGTTCCCATCTGAGTGTCTG BaH33H02 ACTCAGCAAGGTATCAAATAA 20948 EcoRV GATATC ACTCAGCAAGATATCAAATAA bags10e02 CGTTGGTGCTCGCGTACGTCA 20976 BstUI CGCG CGTTGGTGCTTGCGTACGTCA bags35n03 TGAATATCAGTTGGCATTTCC 20980 AluI AGCT TGAATATCAGCTGGCATTTCC BaAL15N07 AGGCGACAAAGATCCACACCA 20982 MboI GATC AGGCGACAAAAATCCACACCA BaAL15N07 AGACTGGTGATCTGGAGCCGG 20983 MboI GATC AGACTGGTGACCTGGAGCCGG bags37g12 CAGAATAACCACGCATGTACC 20986 HhaI GCGC CAGAATAACCGCGCATGTACC bags22l12 GGCCCACGAAGCTTAGAGGAT 20987 AluI AGCT GGCCCACGAAACTTAGAGGAT BaAK44K01 GGTTTTGAGTACTTGTGTTTG 20988 AfaI GTAC GGTTTTGAGTGCTTGTGTTTG BaAK31G07 CATCAGAGATTGCAGGAACCT 20989 MboI GATC CATCAGAGATCGCAGGAACCT bah53n21 TTGAGTGATTTGATGATGCTT 20990 HinfI GANTC TTGAGTGATTCGATGATGCTT kr29d0107 TGCTCGAGCTCTAGGGTATTT 21005 XspI CTAG TGCTCGAGCTGTAGGCTATTT bah52iC3 CGTCGGAGAGTCCAAGGCCCC 21019 BanII GRGCYC CGTCGGAGAGCCCAAGGCCCC BaAK33K05 TGTTGCTCCCGGTCACAACAT 21020 BcnI CCSGG TGTTGCTCCCTGTCACAACAT bast12G0113 TTCAGCTGGTGCTAAGGGTAT 21028 AfaI GTAC TTCAGCTCGTACTAAGGGTAT baak13k16 AGCCACTCGAGAAGCAGGACT 21041 XhoI CTCGAG AGCCACTCGAAAAGCAGGACT bags1h15 CTGGATAGCGCGAGGGAGCTA 21047 HhaI GCGC CTGGATAGCGTGAGGGAGCTA kr26H0515 CCACAGGGCTGGAGCTCAACA 21048 XspI CTAG CCACAGGGCTAGAGCTCAACA BaH32G11 GTCAGATGTGGTCCACCACGT 21054 Cfr13I GGNCC GTCAGATCTGATCCACCACGT BaGS36A16 GGTGGTCCGCGGTGGCTGGCT 21065 SacII CCGCGG GGTGGTCCGCAGTGGCTGGCT baak1202 TAAGCATTCCGGTGCAAATCC 21073 HapII CCGG TAAGCATTCCAGTGCAAATCC bags1m19 ATATGTTCATAGATGTCAATC 21082 FokI GGATGNNNNNNNNN ATATGTTCATGGATGTCAATC

TABLE 20-1 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd14f03 ATCATTCAGGATGTAAAATGT 21083 FokI GGATGNNNNNNNNN ATCATTCAGGGTGTAAAATGT BaAL30P12 CCTCCTCCTCGGGTGTAAAGT 21093 AvaI CYCGRG CCTCCTCCTCAGGTGTAAAGT BaGS21H12 AGTAATTTGATCCACAAGTAT 21094 MboI GATC AGTAATTTGAACCACAAGTAT BaGS9G15 TAGGACCTGACCTTTCAAGCT 21095 MboI GATC TAGGACCTGATCTTTCAAGCT baak41j04 CCCGGCACCTGTCAGACACGC 21096 DdeI CTNAG CCCGGCACCTCTCAGACACGC bags38a05 TTCCTACTCAGATCTGCCAGG 21099 MboI GATC TTCCTACTCAAATCTGCCAGG BaH43I06 CGCGGCCGCGCGCCTGCCACA 21100 HhaI GCGC CGCGGCCGCGTGCCTGCCACA bags9j07 TTCACACTTAAAAGGCACACA 21101 DdeI CTNAG TTCACACTTAGAAGGCACACA baak31p11 TTGCGCCTTCGAACAATGAAT 21102 BspT104I TTCGAA TTGCGCCTTCAAACAATGAAT BaGS17P19 GATGGTCCTCAATTTCACTTG 21103 TaqI TCGA GATGGTCCTCGATTTCACTTG baal32j05 CGAGGCCTCTTGCGACGTAGA 21104 BstUI CGCG CGAGGCCTCTCGCGACGTAGA baal16l16 ACTGACTCGCGATTTATTTTA 21105 BstUI CGCG ACTGACTCGCAATTTATTTTA baal6a11 TCACCGTGGATCTCCCGCCCA 21106 MfII RGATCY TCACCGTGGACCTCCCGCCCA bah55p06 ACGGTCTCTCATCGATGCGCT 21107 ClaI ATCGAT ACGGTCTCTCGTCGATGCGCT bags8f03 TTCAGGGCCAAAGTCTCCACC 21108 HinfI GANTC TTCAGGGCCAGAGTCTCCACC BaAL4B09 CAACACCAATATAGAAATCAG 21117 XbaI TCTAGA CAACACCAATCTAGAAATCAG bags32f21 AAGTGAAGATCTCAGTAATGA 21118 MfII RGATCY AAGTGAAGATTTCAGTAATGA BaGS10G07 TGTTGGGGATGACGCCGCCGT 21121 FokI GGATGNNNNNNNNN TGTTGGGGATCACGCCGCCGT BaH63O04 GTAAGTCCGCAACACATGGCG 21125 BstUI CGCG GTAAGTCCGCGACACATGGCG BaG326D21 AGGTATTGAGTTCAAGTGAGA 21126 MboI GATC AGGTATTGAGATCAAGTGAGA baal4k16 GTAAATCCATTGATTCATGCA 21138 ClaI ATCGAT GTAAATCCATCGATTCATGCA bags32b14 GTCACGGCCGGCCTCTTCCGC 21139 HapII CCGG GTTACGGCCGCCCTCTTCCGC baal4d01 AGTTTAAGGCCCCGAAGGTTC 21140 HaeIII GGCC AGTTTAAGGCTCCGAAGGTTC baal4d01 TGCT--AAGGTCCTTGGATGA 21141 HaeIII GGCC TGCTATAAGGGCCTTGGATGA 21142 baak25d07 CATCGTGAGGGGCGGTGGAGT 21154 HhaI GCGC CATCGTGAGGCGCGGTGGAGT

TABLE 20-2 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAK29M05 AGACGCAGGATCTGTCGAAGT 21155 AvaII GGWCC AGACGCAGGACCTGTCGAAGT BaGS31I08 CTCAAGCATGCGCTTCTTTGC 21156 HhaI GCGC CTCAAGCATGTGCTTCTTTGC baal30o06 CCTTCTTCTCACCGGCGGCGC 21158 HphI NNNNNNNTCACC CCTTCTTCTCTCCGGCGGCGC baak13n14 GCGAGCACCGCCGGATAAACT 21159 HapII CCGG GCGAGCACCGTCGGATAAACT bags7a09 TAAAAGACAGCTTGCTAACTC 21160 AluI AGCT TAAAAGACAGTTTGCTAACTC bah31o16 GTGCTTCAACACGATTTAGCA 21166 BstUI CGCG GTGCTTCAACGCGATTTAGCA kr40D0707 GTCTTCCGGACGTTGAGGAAA 21170 FokI GCATGNNNNNNNNN GTCTTCCGGATGTTGAGGAAA BaAL30H03 AGAAACTGGGAAATTCTGGGT 21175 EcoRI GAATTC AGAAACTGGGGAATTCTGGGT basd3f22 TAGTCCAAGGGCACATCACAC 21192 Bsp1286I GDGCHC TAGTCCAAGGACACATCACAC BaAK41K07 GCTGGTCCGGNGGGATGCCTT 21196 HapII CCGG GCTGGTCCGGGGGGATGCCTT BaAK41K07 TGGTCTTGCCGGTGAGGGTCT 21197 HapII CCGG TGGTCTTGCCTGTGAGGGTCT BaH53I11 ATCATCTCCTTGAGCACCAGC 21198 MvaI CCWGG ATCATCTCCTGGAGCACCAGC BaAL18N03 TCGGAACTCCGGACGGGCTCG 21201 HapII CCGG TCGGAACTCCAGACGGGCTCG baak40c08 ATATATAACANGAAGAGAGCT 21202 AluI AGCT ATATATAACAAGAAGAGAGCT baak40c08 CTGCATTGCAGCTCTGTGTTA 21203 AluI AGCT CTGCATTGCAACTCTGTGTTA baal33h17 TAGACCGATAGATCCTACTTA 21207 MfII RGATCY TAGACCGATAAATCCTACTTA bags20d08 TCAGGACGGCCTTGTCAACCT 21208 HaeIII GGCC TCAGGACGGCTTTGTCAACCT BaGS4J01 TCGCGCCGTAAGCCTCGGTGC 21209 HaeIII GGCC TCGCGCCGTAGGCCTCGGTGC BaAK17101 GTGGGTCCACAATCTCACCGA 21215 MboI GATC GTGGGTCCACGATCTCACCGA BaAK30F08 TTGCTCAGAGCCTGGGCCGCG 21216 HinfI GANTC TTGCTCAGAGTCTGGGCCGCG bags19g17 TGACAGCAGCCGATGTCGTGC 21225 AluI AGCT TGACAGCAGCTGATGTCGTGC baak21l02 CGTCCCATTCGAGGGTATCGG 21226 TaqI TCGA CGTCCCATTCAAGGGTATCGG bags9l13 ATATGTCCGCAAAAGGCGTTC 21232 BstUI CGCG ATATGTCCGCGAAAGGCGTTC baak12i20 CAAGAGACGCGGAGGATGACA 21241 BstUI CGCG CAAGAGACGCAGAGGATGACA BaAK3M04 AGCAAGGTACATCCAATACTT 21242 BanI GGYRCC AGCAAGGTACCTCCAATACTT

TABLE 20-3 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bah15n23 GGCAGGGCAGATTTCCCACAA 21244 AluI AGCT GGCAGGGCAGCTTTCCCACAA baak45i02 CCGCAAGGGTGCGACCATCCT 21245 AvaII GGWCC CCGCAAGGGTCCGACCATCCT BaH45B01 AACCAAGCACAGTACCAGGAC 21253 KpnI GGTACC AACCAAGCACGGTACCAGGAC baal23o14 CCGCAAGGGTGCGACCATCCT 21255 AvaII GGWCC CCGCAAGGGTCCGACCATCCT baal4d14 AAGACTGAAAAAGCACCCCCT 21271 Bsp1286I GDGCHC AAGACTGAAAGAGCACCCCCT baak29j13 AGCAACCTTACAAACTGAAAG 21280 MseI TTAA AGCAACCTTAAAAACTGAAAG bags20p18 GTTTCGACTCGGATGCCGCTC 21281 FokI GGATGNNNNNNNNN GTTTCGACTCAGATGCCGCTC BaAK12J13 TGGTGACTCCGGTGATGCTGA 21282 HapII CCGG TGGTGACTCCCGTGATGCTGA BaH27N11 ACTAACCATGGAAAAGGCTTA 21286 NcoI CCATGG ACTAACCATGTAAAAGGCTTA BaGS34D11 CTATCTGCCAGATCCTGTCAC 21287 MflI RGATCY CTATCTGCCATATCCTGTCAC BaGS39G07 ATTTTGTCATTCAGATAATTT 21288 FokI NNNNNNNNNNNNNCATCC ATTTTGTCATCCAGATAATTT BaGS32G02 ATGCTGAAAGGACTCTTCTAG 21320 HinfI GANTC ATGCTGAAAGAACTCTTCTAG bast21C1105 TATATCCGTCAATATGTGGTA 21326 TaqI TCGA TATATCCGTCGATATGTGGTA bags29f03 CGCCATTAGGTGCTATGGAGG 21327 AluI AGCT CGCCATTAGGAGCTATGGAGG BaAK46B16 TCTGAACTGTCGATATGCCTA 21328 TaqI TCGA TCTGAACTGTGGATATGCCTA baal16h16 TTTCTTTCTGACGCAAGCAAA 21329 HhaI GCGC TTTCTTTCTGGCGCAAGCAAA BsAL13O24 CGCAGTTTTCCACTTGATTCT 21338 TaqI TCGA CGCAGTTTTCGACTTGATTCT BaAL20M22 GCGTCCTTGAACGCCGCTCTC 21347 HaeII RGCGCY GCGTCCTTGAGCGCCGCTCTC BaAL151B12 TCAAGAAGCATCCCTGAATAT 21353 FokI NNNNNNNNNNNNNCATCC TCAAGAAGCAGCCCTGAATAT bags39h18 TTTACCTGTTTGAATTTGCAA 21360 BspT104I TTCGAA TTTACCTGTTCGAATTTGCAA BaAL3L23 TCCACCTCACCAAGGAATTCA 21362 DdeI CTNPCT TCCACCTCACTAAGGAATTCA BaH50L11 ATCATAAAATCTTTTGAATCA 21380 SspI AATATT ATCATAAAATATTTTGAATCA bast50G0814 CTACAGCACAGTACTCTGCCG 21381 ScaI AGTACT CTACAGCACACTACTCTGCCG BaH46B06 AGACTCTGTCCCGGACTCCAG 21403 HapII CCGG AGACTCTGTCACGGACTCCAG baal5e24 TGAAGCGGATCGATCTAATGG 21404 ClaI ATCGAT TGAAGCGGATTGATCTAATGG

TABLE 20-4 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bags11a16 GTTGTCCTGCCTAGGGTCGCC 21405 EcoT141I CCWWGG GTTGTCCTGCTTAGGGTCGCC BaGS37K02 TCTTTGTCACGCGAACTGCCG 21413 BstUI CGCG TCTTTGTCACACGAACTGCCG bah28a10 ACTATCAGAGTCACACTATGA 21414 HinfI GANTC ACTATCAGAGCCACACTATGA bah27c03 GGCTGCCATCGGATGCAAACG 21415 FokI NNNNNNNNNNNNNCATCC GGCTGCCATCCGATGCAAACG bags37g15 GAGCTTTCGCGCTAAGTGACA 21418 BstUI CGCG GAGCTTTCGCACTAAGTGACA BaH54L03 CACACGGCGATCGGAGCTTCA 21426 MboI GATC CACACGGCGACCGGAGCTTCA BaAK21J17 GTGCATCGTAAATCATCAGGG 21427 AfaI GTAC GTGCATCGTACATCATCAGGG bags23g08 ATGATGCAGGCGCTCAGTGGA 21428 HhaI GCGC ATGATGCAGGGGCTCAGTGGA BaAL2M19 CCTAGTGGGCATCCTCCTCCT 21440 FokI NNNNNNNNNNNNNCATCC CCTAGTGGGCGTCCTCCTCCT BaH36F15 CGAGGCCCTGGGCGACGTACC 21442 MvaI CCWGG CGAGGCCCTGCGCGACGTACC bah58l07 GCTCAAACTGTGCTGACAGGC 21443 HhaI GCGC GCTCAAACTGCGCTGACAGGC baet29C0406 ACGGGAGCGACCGGCACCCAC 21444 HapII CCGG ACGGGAGCGAGCGGCACCCAC BaAK35804 ATCCCCTGCCCCGCCTTGAGG 21445 HhaI GCGC ATCCCCTGCCGCGCCTTGAGG bags34k13 ACAATTCTGACCGAACATAGC 21446 TaqI TCGA ACAATTCTGATCGAACATAGC baal29i08 CCTGTTGCTGCAGCTATATCT 21447 PstI CTGCAG CCTGTTGCTGTAGCTATATCT baak11pl0 AAACGAGATGTGCTGTCTAGT 21448 HhaI GCGC AAACGAGATGCGCTGTCTAGT bags4e12 GGAACTTGCGGAGTTTCAGGA 21449 HhaI GCGC GGAACTTGCGCAGTTTCAGGA basd24b08 TAAAGATTGGCCTGATTTGTT 21457 HaeIII GGCC TAAAGATTGGTCTGATTTGTT baalf3o10 GTCGATCTCCGGCTGCGCCAC 21463 AluI AGCT GTCGATCTCCAGCTGCGCCAC bags15d14 CTATTTTTTANAGGATGGGAC 21471 FokI GGATGNNNNNNNNN CTATTTTTTAAAGGATGGGAC bags15d14 CTTTGTCATCCGTATTTCTTA 21472 FokI NNNNNNNNNNNNNCATCC CTTTGTCATCTGTATTTCTTA BaGS37E09 GGGCAGAGTCTCGCCAGTAGC 21473 HhaI GCGC GGGCAGAGTCGCGCCAGTAGC BaGS19I11 ATGCTGTTAATGCTTGACGAT 21481 HindIII AAGCTT ATGCTGTTAAAGCTTGACGAT BaGS18P04 ATTCACTGGTACAGACGATGA 21491 AfaI GTAC ATTCACTGGTGCAGACGATGA bags19e06 GCGAGTAGATCATTAAGAATT 21492 MboI GATC GCGAGTAGATTATTAAGAATT

TABLE 20-5 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence baak35n07 TTCCGCTCTCACCAACCGAGG 21493 HphI NNNNNNNTCACC TTCCGCTCTCCCCAACCGAGG BaAK14C17 GTGCAGGCCGACGAACAATGG 21519 TaqI TCGA GTGCAGGCCGTCGAACAATGG BaAK1N06 CAATTGCATCCTAGTTAGCCG 21525 XspI CTAG CAATTGCATCTTAGTTAGCCG bags38d10 ATACCTCAGCTAATCTCTGGC 21526 AluI AGCT ATACCTCAGCCAATCTCTGGC BaAK19J15 GGAAGGTGGGTACCTCCACGA 21527 AfaI GTAC GGAAGGTGGGCACCTCCACGA BaGS37D24 TCGTTCAGGGACAAGGAGTTG 21544 HaeIII GGCC TCGTTCAGGGCCAAGGAGTTG BaGS9K15 GAAACTTCCACGGAAGGACTG 21545 MvaI CCWGG GAAACTTCCAGGGAAGGACTG BaGS33L03 ACACACGCCGTCGGAAGGCCC 21546 SacII CCGCGG ACACACGCCGCGGGAAGGCCC kr58F0511 GCTTCGTTGTGTACTGGGTCT 21548 AfaI GTAC GCTTCGTTGTTTACTGGGTCT baak39o18 AGCCAACATGATACTCTCTAT 21566 AfaI GTAC AGCCAACATGGTACTCTCTAT bags20p12 GCACCCGGGCTCCGACCACGT 21567 BanII GRGCYC GCACCCGGGCGCCGACCACGT bags38f23 TCAATGATAGCTACTTTCAAG 21568 AluI AGCT TCAATGATAGTTACTTTCAAG bags3k09 TGAACATACAATGAGAGAGCT 21575 TspRI CASTGNN TGAACATACAGTGAGAGAGCT baak34p14 CAATGTCCCGGAGGATACGGA 21580 BcnI CCSGG CAATGTCCCGAAGGATACGGA basd15m11 CAGCACAGGTCCATAGATGGA 21583 AvaII GGWCC CAGCACAGGTTCATAGATGGA bags21b06 AAGAACAGCTAAAACATATAT 21584 PvuII CAGCTG AAGAACAGCTGAAACATATAT BaH18F07 TGGGGGCGGCGCCCAGCACTC 21585 HhaI GCGC TGGGGGCGGCTCCCAGCACTC BaH44A23 CAGCATCTTCGAATGCTGAGA 21586 BspT104I TTTCGAA CAGCATCTTCAAATGCTGAGA bags3d07 AGACACCGACCGCAGTTGACG 21587 PstI CTGCAG AGACACCGACTGCAGTTGACG baak24e07 CGGTAGGCACCCAAGAGCTCC 21589 BanI GGYRCC CGGTAGGCACGCAAGAGCTCC

TABLE 21-1 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bah42i06 CTTGCTTGCTCACATGGTGAG 21600 DraIII CACNNNGTG CTTGCTTGCTGACATGGTGAG bagsl2l21 TCCTTGCATCTGTCTCACCAT 21629 FokI NNNNNNNNNNNNNCATCC TCCTTGCATCCGTCTCACCAT baak12j24 GTTCGGCCCGTGGAAAGGCAG 21630 SacII CCGCGG GTTCGGCCCGCGGAAAGGCAG bags14d22 TTAGGAAGAGGGCGAGATAGG 21631 BstUI CGCG TTAGGAAGAGCGCGAGATAGG baal6b02 AGTGTTCCGGGAGAAGCTCCG 21633 BcnI CCSGG AGTGTTCCGGCAGAAGCTCCG baak29f13 AGATCCACTAACATCTCCGTA 21634 XspI CTAG AGATCCACTAGCATCTCCGTA bags19e04 TTATAAGGCCGTAAATCTAAA 21643 Cfr13I GGNCC TTATAAGGCCCTAAATCTAAA BaH28L07 CCATGCTTCCCAGGACAGAGC 21644 MvaI CCWGG CCATGCTTCCGAGGACAGAGC baak21m18 TGATGATCCGCGGGGGAAGGT 21645 SacII CCGCGG TGATGATCCGTGGGGGAAGGT BaH59A20 AGTTCTGGGATGGCGGGTTGA 21657 FokI GGATGNNNNNNNNN AGTTCTGGGACGGCGGGTTGA BaAK31N06 AGATTTTGTTGACTGACCGAC 21662 TspRI CASTGNN AGATTTTGTTCACTGACCGAC bags29c18 ATACTACTGAAACACGATATC 21666 DdeI CTNAG ATACTACTGAGACACGATATC bags37n23 GCCGGCGTTAACAGAGCCAGC 21670 MseI TTAA GCCGGCGTTAGCAGAGCCAGG BaGS21E20 AAACAACACGACCGAGACGGT 21681 HaeIII GGCC AAACAACACGGCCGAGACCGT BaAK37P20 ATTGCTTCTCAGA-TCAAACC 21694 DdeI CTNAG ATTGCTTCTCGGAGTCAAACC 21695 bah34b22 TACCGTCGCTTAAATACCATC 21699 MseI TTAA TACCGTCGCTNAAATACCATC bah34b22 TCTTCACTTAAACAAATCCAG 21700 MseI TTAA TCTTCACTTACACAAATCCAG baak42a24 ATGACTCCGGAGCTTTACCAA 21708 HhaI GCGC ATGACTCCGGCGCTTTACCAA baak16f06 AAGCAGCACAGCGCCGTGAAC 21709 HhaI GCGC AAGCAGCACAACGCCGTGAAC baak16f06 TGGGGGTCTCCGCTGCGCCTT 21710 HhaI GCGC TGGGGGTCTCTGCTGCGCCTT bah11m12 GTGCTAGCTAGTAATATGCAG 21711 AsaI ATTAAT GTGCTAGCTATTAATATGCAG baak33d06 TTCAGACACTAATGCTCCGTA 21719 TspRI CASTGNN TTCAGACACTGATGCTCCGTA BaGS25G03 ACAGCTCTGGACTGTGGTGGC 21725 HaeIII GGCC ACAGCTCTGGCCTGTGGTGGC bast42C0406 AGTGCCTCCTCGCCAGCGACA 21728 XspI CTAG AGTGCCTCCTAGCCAGCGACA

TABLE 21-2 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bagsl7i04 TGCAACAGATGCGTCTAACTT 21758 MboI GATC TGCAACAGATCCGTCTAACTT baal40b06 AGACTCCGTCAACTTCCAGCA 21762 TaqI TCGA AGACTCCGTCGACTTCCAGCA baal40b06 TCCATCTGCAATTTCAGTAAA 21763 TaqI TCGA TCGATCTGCATTTTCAGTAAA baak3c01 CGGGCCGTAGCTGGCCTGGTT 21770 AluI AGCT CGGGCCGTAGGTGGCCTGGTT baak3c01 CTCCTTGAGCTTGAACGGTCC 21771 AluI AGCT CTCCTTGAGCCTGAACGGTCC BaH52L11 GACATGACTTGGAACTATCCT 21788 DdeI CTNAG AACATGACTTAGAACTATCCT BaH52L11 GTGTACCTGAGCATT-CAGAA 21789 DdeI CTNAG GTGTACCTGACCATTTCAGAA 21790 BaH52L11 TCAGCCTCTCTGAGTAGAGCT 21791 DdeI CTNAG TCAGCCTCTCCGAGTAGAGCT baalI8b16 TTGCTCCCCTCATCAGAGGTA 21792 MboI GATC TTGCTCCCCTGATCAGAGGAA bah60l22 CGCCAAACTAGTAGGACAAAA 21801 XspI CTAG CGCCAAACTAATAGGACAAAA bags21m22 TAAAAAGAAGCACAATATGAT 21802 AfaI GTAC TAAAAAGAAGTACAATATGAT BaH54E07 GGCAGTACACGAGCTTCCGAG 21803 HindIII AAGCTT GGCAGTACACAAGCTTCCGAG BaAK39A20 AGTCTTTTTAAAGATTCCACT 21804 DraI TTTAAA AGTCTTTTTAGAGATTCCACT baet32B1103 CAGTTCCTCTAGAACAGAGCT 21810 XbaI TCTAGA CAGTTCCTCTGGAACAGAGCT BaAK31O16 TATATGAAGCGCTCCAGCACG 21811 HhaI GCGC TATATGAAGCACTCCAGCACG bags39l20 TGTCCTGAGTTGCAGCCCTTG 21812 HinfI GANTC TGTCCTGAGTCGCAGCCCTTG BaGS15L12 ATCTCTTCCTCGGGAACCGCT 21813 AvaI CYCGRG ATCTCTTCCTTGGGAACCGCT bags11h08 CAATGCGGTACCCCTCGACGG 21831 AfaI GTAC CAATGCGGTATCCCTCGACGG BaAK13L03 CAAGAAAGTACTTTTGGAAAA 21851 AfaI GTAC CAAGAAAGTAATTTTGGAAAA basd20j22 ACAGCAGGTACTGTGATTAAG 21854 AfaI GTAC ACAGCAGGTATTGTGATTAAG BaGS38F01 TGAGACATCTTAAACCTTCAC 21861 MseI TTAA TGAGACATCTCAAACCTTCAC BaAK32I05 CCCCAAACTAATAGATGAAAA 21862 XspI CTAG CCCCAAACTAGTAGATGAAAA BaAK14F01 TTCAAACATCGGCGGAAGATT 21870 SacII CCGCGG TTCAAACATCCGCGGAAGATT bah57o01 TCTCGCCGGCATCCTCCTCCA 21871 FokI NNNNNNNNNNNNNCATCC TCTCGCCGGCGTCCTCCTCCA

TABLE 21-3 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence basd12f05 GCACCGGGAGCTCTTGGCTCC 21872 SacI GAGCTC GCACCGGGAGTTCTTGGCTCC bah58e21 TAAACTTGGTGCTCCTGTAAC 21873 Bsp1286I GDGCHC TAAACTTGGTTCTCCTGTAAC bags1d07 CAATGAGGATTCTATGTGGTG 21874 MboI GATC CAATGAGGATCCTATGTGGTG BaH23J15 TTCAAACATCGGCGGAAGATT 21875 SKII CCGCGG TTCAAACATCCGCGGAAGATT baal18g23 TCTCGCCGGCATCCTCCTCCA 21882 FokI NNNNNNNNNNNNNCATCC TCTCGCCGGCGTCCTCCTCCA BaH50P14 TCAGCGTGGACGTGGTGGAGT 21892 FokI GGATGNNNNNNNNN TCAGCGTGGATGTGGTGGAGT BaAK23L23 TCGGATATCCCGGGTAACCAA 21902 AvaI CYCGRG TCGGATATCCTGGGTAACCAA bags7a23 CCGGCTCATATTGCAGCATTT 21903 PstI CTGCAG CCGGCTCATACTGCAGCATTT bah56l04 AGCAACTCTAACCTACCTCGA 21904 XspI CTAG AGCAACTCTAGCCTACCTCGA BaAL6G08 ATCAAAAACGCGATGCCACCA 21923 BstUI CGCG ATCAAAAACGAGATGCCACCA bast10C0406 TGCTAGGTTCCATCATCCTGC 21931 TaqI TCGA TGCTAGGTTCGATCATCCTGC bah62n05 GAAAAAACGCATGAGTCTAAC 21945 BstUI CGCG GAAAAAACGCGTGAGTCTAAC baak20d11 GTGGCAGTGCGCCGCCAATGG 21958 HhaI GCGC GTGGCAGTGCTCCGCCAATGG BaAL6D19 GTAACTTGGCCTTTCATCGAC 21959 HaeIII GGCC GTAACTTGGCTTTTCATCGAC baak43e16 ACATGTTGTATAGGTCTTCGA 21960 AfaI GTAC ACATGTTGTACAGGTCTTCGA baak40b02 GCAATTTCAGTGCTAGATTGG 21961 AkAI AGCT GCAATTTCAGAGCTAGATTGG bah62m03 AGCTTCGCGGAGCTATGACCT 21962 HhaI GCGC AGCTTCGCGGCGCTATGACCT bah56g23 ACTGTTCCTG-ATGTCGAGAA 21977 MvaI CCWGG ACTGTTCCTGGATGTCGAGAA 21978 bah56g23 GAGAAACCTGGCGAAACCGCA 21979 MvaI CCWGG GAGAAACCTGACGAAACCGCA basd22f14 TTCATTCACAGGCCAGCTTGG 21980 HaeIII GGCC TTCATTCACACGCCAGCTTGG BaAK26G13 CAAGACCGAACTAATCTTGCT 21984 AseI ATTAAT CAAGACCGAATTAATCTTGCT BaAK44D07 CCTCCTCTGGGCCGTCGACGT 21985 HaeII GGCC CCTCCTCTGGTCCGTCGACGT bah34b20 AGATGGTCTCCGATCGGTGGG 21987 DdeI CTNAG AGATGGTCTCAGATCGGTGGG BaAK42B16 AATGCTTTCGAAATCAGTNNT 21992 TaqI TCGA AATGCTTTGGGAATCAGTTTA

TABLE 21-4 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence BaAK42816 GGAAATCTCGGTTTTGAATAC 21993 TaqI TCGA GGAAATCTCGATTTTGAATAC baal4d05 TGCCGGAATCACCAGGTTGGC 22022 HphI NNNNNNNTCACC TGCCGGAATCCCCACCTTCGC basd20l03 AGCGCCAGAGCCCCATCTTGA 22023 Cfr13I GGNCC AGCGCCAGAGGCCCATCTTGA baal31b03 ACTTTCTAGCGCGTGCTTCTG 22024 HhaI GCGC ACTTTCTAGCCCGTGCTTCTG bags27k21 TCCTGGCAGCTGTCAACTATG 22025 AluI AGCT TCCTGGCAGCCGTCAACTATG bah55n15 CAGCAAACTTTAAATGTTTTT 22026 DraI TTTAAA CAGCAAACTTCAAATGTTTTT BaAK20B23 ATCAATACTCAGAAGGCGTTA 22031 DdeI CTNAG ATCAATACTCGGAAGGCGTTA bags22c18 ATGACATTTCGAAATTTTGCT 22036 BspT104I TTCGAA ATGACATTTCAAAATTTTGCT bah29d20 ACCCAAAAGCTCTATCCTACG 22040 AluI AGCT ACCCAAAAGCCCTATCCTACG bah44f20 CCTACACCCGAGCGTAATGGA 22041 BstUI CGCG CCTACACCCGCGCGTAATGGA BaAL4A11 GCTGAGGTACACGCGCTGGAG 22049 KpnI GGTACC GCTGAGGTACCCGCGCTGGAG BaGS22I18 CCAATTTATACTCAGCCATTC 22050 DdeI CTNAG CCAATTTATAGTCACCCATTC BaAK40P18 CCCTTTGGTCAAGGCTCAGAC 22065 TaqI TCGA CCCTTTGGTCGAGGCTCAGAC BaH27K19 TCTAAGAAGGGGACTCGTGTC 22070 BsmAI NNNNNGAGAC TCTAAGAAGGAGACTCGTGTC bags15j07 CATTTTGCCACACATAGTGAG 22071 DraIII CACNNNGTG CATTTTGCCAGACATAGTGAG bahl9a07 GGTCTTGGTCGCGTCGTCGAG 22072 BstUI CGCG GGTCTTGGTCACGTCGTCGAG bah44n05 AGATGAAGCGCCATCGACATA 22103 HhaI GCGC AGATGAAGCGTCATCGACATA BaGS13H12 GACAAAGTTAATCAGTAATTG 22113 MseI TTAA GACAAACTTATTCACTAATTG BaAL19B06 ATATGTTCTCGACAGCCCGCA 22114 TaqI TCGA ATATGTTCTCCACAGCCCGCA BaSD15P23 GTGTCCCTCCGGTGCCTTCTC 22115 BanI GGYRCC GTGTCCCTCCTGTGCCTTCTC kr27H1216 CTGCGTCGTCAACTATCTGAA 22130 SaII GTCGAC CTGCGTCGTCGACTATCTGAA BaAK25O11 GGGCTACTGTCTCTGCATGCT 22131 BsmAI GTCTCN GGGCTACTGTATCTGCATGCT BaH36N15 CACTTCCGGCGCTGGATTTGC 22138 HaeIII GGCC CACTTCCGGCCCTGGATTTGC BaAK38E05 CTTGTATGTCGAGCGACAACG 22144 TaqI TCGA CTTGTATGTCAAGCGACAACG

TABLE 21-5 Restriction Clones Haruna Nijo H602 SEQ ID NO: enzyme Recognition sequence bast60C0105 CAGCCGCCTCATCCAGCGCCC 22152 FokI NNNNNNNNNNNNNCATCC CAGCCGCCTCGTCCAGCGCCC BaAL24O04 CCTGCAACGCGAAGTTCTGGC 22154 BstUI CGCG CCTGCAACGCAAAGTTCTGGC BaGS31G02 CCACAGAAGAGAGCTGGTGAT 22164 PvuII CAGCTG CCACAGAAGACAGCTGGTGAT basd18g14 TGGCATTGGACGTCTCGAACA 22167 FokI GGATGNNNNNNNNN TGGCATTGGATGTCTCGAACA bah11k13 GGCCATCCTCAAGCTGCTTGC 22178 XhoI CTCGAG GGCCATCCTCGAGCTGCTTGC bags32a01 GCGCTTTCCCAGATCCCTTAG 22184 HapII CCGG GCGCTTCCCCGGATCCCTTAG bags32a01 GAGTAACCGCTGCCATTGTTG 22185 HapII CCGG GAGTAACCGCCGGCATTGTTG baal37j12 CATAGCCTTCCACGCTAGCAT 22193 TaqI TCGA CATAGCCTTCGACGCTAGCAT BaAK40H10 TATTGAGCATTGAGCCCTCTC 22195 TaqI TCGA TATTGAGCATCGAGCCCTCTC BaAK40H10 TTAAGAACTTCGAATCATCAC 22196 TaqI TCGA TCAAGAACTTTGAATCATCAC bah56p08 GCTTATCATCCCGGTCGTATC 22203 HapII CCGG GCTTATCATCACGGTCGTATC bags1i05 ACAGAGACTACGGCATCACTG 22204 XspI CTAG ACAGACACTAGGGCATCACTG BaH29M21 CCAGCAGCTCTAGGCTTGCGC 22205 MvaI CCWGG CCAGCAGCTCCAGGCTTGCGC bags21c04 AGCATGAGCCTGCTTACATCC 22206 AluI AGCT ACCATGAGCCAGCTTACATCC baak32p09 CGCCTTCTGCAGGGTCTTCGA 22207 PstI CTGCAG CGCCTTCTGCGGGGTCTTCGA BaH22B15 ACACAAACTCCGGTGAAGCAA 22221 HapII CCGG ACACAAACTCTGCTCAAGCAA BaGS23J01 TGCCGATGTCGGGGTGCACCT 22239 HapII CCGG TGCCGATGTCCGGGTGCACCT baak46i07 GCCACCCTCGCGATGATGTCT 22242 BstUI CGCG GCCACCCTCGTGATGATGTCT BaAL6J13 GATCTCACTGTACCGCGAGCC 22243 AfaI GTAC GATCTCACTGCACCGCGAGCC BaH54J20 ATTTCAGCCAGGGTCATCATC 22254 NcoI CCATGG ATTTCAGCCATGGTCATCATC BaGS7F20 AGTCAATCACGATTCAAGGCG 22255 HinfI GANTC ACTCAATCACAATTCAAGGCG bags11p11 CCTTTGTTTCGACAACATGTC 22256 TaqI TCGA CCTTTGTTTCAACAACATGTC bags11p11 CTTCTCATCGTGCAGAACAAG 22257 TaqI TCGA CTTCTCATCGAGCAGAACAAG baak26h17 TTTCCTGGTCCGGATTGGACT 22277 HapII CCGG TTTCCTGGTCTGGATTGGACT

TABLE 21-6 Haruna Nijo SEQ ID Restriction Recognition Clones H602 NO: enzyme sequence BaGS4H02 CGGCGAGGTACCCTCCTCCAC 22295 KpnI GGTACC CGGCGAGGTATCCTCCTCCAC BaSD14J15 CAAACATGACTGGTCCTAATG 22302 Cfr13I GGNCC CAAACATGACAGGTCCTAATG BaSD14J15 GTTGACGGCCTCTCGTGAACA 22303 Cfr13I GGNCC GTTGACGGCCCCTCGTGAACA bags38n21 AGAGCCCGCTCGAGTTTACAC 22314 XhoI CTCGAG AGAGCCCGCTTGAGTTTACAC BaH49M02 CAGCACGTTCCAAACAATAGG 22318 BspT104I TTCGAA CAGCACGTTCGAAACAATAGG baak38f04 CCCCCTCGCCTGCTCTTCTCC 22319 HapII CCGG CCCCCTCGCCGGCTCTTCTCC basd15d20 TCATGGAGTCGTACCTCATGA 22324 AfaI GTAC TCATGGAGTCATACCTCATGA kr40H1115 AAGTAAATTAAATTCAGAGTA 22329 HinfI GANTC AAGTAAATTAGATTCAGAGTA

TABLE 22-1 Haruna Nijo SEQ ID Clones H602 NO: bags30b07 GCAACCACGACGTACATCGTT 17341 GCAACCACGAGGTACATCGTT  BaAK17D13 AGCTCGCGGCACCGTTCACCT 17343 AGCTCGCGGCGCCGTTCACCT basd26l20 CCTCCACGACCGCCCCGGGCT 17344 CCTCCACGACGGCCCCGGGCT bah61p17 ATAATGTATCCACATTCTCGC 17345 ATAATGTATCGACATTCTCGC bah61p17 TTTTCTGAAACGCAAACAATA 17346 TTTTCTGAAATGCAAACAATA bah61p17 CGCTTGCCCACGTGTGCAACA 17347 CGCTTGCCCAAGTGTGCAACA kr12h0216 TCCTCGGCGCAACCGCCTACA 17348 TCCTCGGCGCGACCGCCTACA kr12h0216 CACTTGTATGTAGTTCACCAA 17349 CACTTGTATGCAGTTCACCAA kr12h0216 ACCAGAGCAACTGACTCACTG 17350 ACCAGAGCAAATGACTCACTG kr12h0216 ACTTCACCCTTGTCCAGCACA 17351 ACTTCACCCTCGTCCAGCACA kr12h0216 GCGGCATCTTCCTATGGTACT 17352 GCGGCATCTTTCTATGGTACT kr26d0507 TGCTAACATGTTTGTACGACC 17357 TGCTAACATGCTTGTACGACC kr26d0507 TCCACTCCATACTCCAGTGGT 17358 TCCACTCCATGCTCCAGTGGT baak41n21 ACCAGACGGGAGACTACGAGG 17360 ACCAGACGGGCGACTACGAGG kr24b0903 ACGCGTAAACTCCAAGTGCAT 17362 ACGCGTAAACGCCAAGTGCAT kr24b0903 TATTCAAGAACAAAACACATG 17363 TATTCAAGAAGAAAACACATG kr24b0903 AGTGCATTGATAGACTCCCCA 17364 AGTGCATTGACAGAGTCCCGA kr24b0903 CTGGAACCTGACTGCTAAAAG 17365 CTGGAACCTGGCTGCTAAAAG kr24b0903 TGCTGGAAACGAAAGTGGGGC 17366 TGCTGGAAACTAAAGTGGGGC baal17o01 CAAACATTAGCCAGTTACTTC 17367 CAAACATTAGTCAGTTACTTC baak41a04 GCTCTCAAGCGTCTGTTCATC 17368 GCTCTCAAGCATCTGTTCATC baak41a04 AAATAAAACAGGAGGAAAGGA 17369 AAATAAAACAAGAGGAAAGGA baak41a04 ATATGTCAGACATGCGTGGCA 17370 ATATGTCAGAAATGCGTGGCA bags16g18 CTCGCGGACGTACCCACCATT 17371 CTCGCGGACGCACCCACCATT bags16g18 CAGGTCTGGGTTTGCCAGGAA 17372 CAGGTCTGGGATTGCCAGGAA

TABLE 22-2 Haruna Nijo SEQ ID  Clones H602 NO: BaSD3C22 AGCGTCCCTTGCCGGGATGGC 17373 AGCGTCCCTTACCGGGATGGC BaSD3C22 TGACGCAGTACCTTGATGCAA 17374 TGACGCAGTATCTTGATGCAA basd27b10 GGAGCGACGACCTTGATCTTC 17376 GGAGCGACGAACTTGATCTTC basd27b10 GGCGTATCCCAAGAGTACCCA 17377 GGCGTATCCCGAGAGTACCCA bah47d23 TGTCACCGGCATCAGCATAGT 17378 TGTCACCGGCGTCAGCATAGT bah47d23 GGTTTGGCGCGATGTCACCGG 17379 GGTTTGGCGCAATGTCACCGG bah47d23 GGGCCCTGTGCGCTTGGTTTG 17380 GGGCCCTGTGTGCTTGGTTTG bah47d23 CTGGTTCTTCGCACCACTGGT 17381 CTGGTTCTTCACACCACTGGT BaH17D02 CATGACCACCATCTCGTGCCG 17383 CATGACCACCGTCTCGTGCCG BaH17D02 TGCATCATCCCTGACTACTTC 17384 TGCATCATCCGTGACTACTTC BaAK12I12 TCCCATCATGGTATGTTCAAC 17389 TCCCATCATGTTATGTTCAAC BaAK12I12 GTTCTAAGGCACTTTAATAAT 17390 GTTCTAAGGCTCTTTAATAAT BaAK12I12 TGAACCGATTTGATACACTGC 17391 TGAACCGATTCGATACACTGC baal6n04 CCGTTGGAACGCACGGGCTCT 17392 CCGTTGGAACACACGGGCTCT baal6n04 CTTTGGAGTAGCCAAGCTCTC 17393 CTTTGGAGTATCCAAGCTCTC baal6n04 CCACGGTGGCAAACTTGTCGT 17394 CCACGGTGGCGAACTTGTCGT baal6n04 ACCTCCAGACGGCGGTTGTCT 17395 ACCTCCAGACAGCGGTTGTCT baal6n04 CCGTGTCCGAAGCTCGCCTCA 17396 CCGTGTCCGAGGCTCGCCTCA baal6n04 GGCACCCTGCAACTCATCTGT 17397 GGCACCCTGCGACTCATCTGT baal16l05 GAAGGGATTGGAGAGTATGTC 17398 GAAGGGATTGCAGAGTATGTC baal16l05 CTATCACTCTTATCAGTGAAG 17399 CTATCACTCTGATCAGTGAAG BaSD3J13 AGTGACGCTGCCTTCTGGGCC 17400 AGTGACGCTGTCTTCTGGGCC BaSD3J13 CGCCCAGACGAGGGTCCAGCA 17401 CGCCCAGACGGGGGTCCAGCA BaSD3J13 CCTTGGCGTCATGGAGCTGCT 17402 CCTTGGCGTCGTGGAGCTGCT BaAK16M07 GCTTCGCCCTCGCTGTAGACG 17406 GCTTCGCCCTTGCTGTAGACG

TABLE 22-3 Haruna Nijo SEQ ID Clones H602 NO: BaAK16M07 TCATTGCTTCCTGCACACCAT 17407 TCATTGCTTCATGCACACCAT BaH36M15 CTGACCATGCAACGAAGAATA  17408 CTGACCATGCGACGAAGAATA baal39c22 AGCACACAGCCGAGGCTCGCT 17409 AGCACACAGCAGAGGCTCGCT kr68b0303 CCTCGTCCTCCTCGCCGGAGA 17410 CCTCGTCCTCGTCGCCGGAGA baak24d09 GGCGAGGTACCGCACGACCTG  17411 GGCGAGGTACGGCACGACCTG baak24d09 GCACGACCTGGCTCCTCTTCC 17412 GCACGACCTGCCTCCTCTTCC baal19i12 GGCTTACAGTCGACAGTAATC 17418 GGCTTACAGTTGACAGTAATC baal19i12 GAATCTGGGTTGGATGATGGC 17419 GAATCTGGGTCGGATGATGGC baal19i12 ATGGCACACCTTTGTTCAATG 17420 ATGGCACACCCTTGTTCAATG baal19i12 GAAACATCTATTAGGCTTACA 17421 GAAACATCTACTAGGCTTACA kr18g0814 GTGAAATTGGCGGGTGGGGAT 17425 GTGAAATTGGTGGGTGGGGAT BaGS8B13 TGGTGCCAGCACCGTCGACGG 17427 TGGTGCCAGCGCCGTCGACGG BaGS8B13 TACCGATCCCAATAAGCGCCT 17428 TACCGATCCCGATAAGCGCCT BaGS8B13 AAGCGGACGAGCGCCTCTTCA 17429 AAGCGGACGAACGCCTCTTCA BaGS8B13 TTGGTGAGCAGATTCTTACCG 17430 TTGGTGAGCACATTCTTACCG BaGS8B13 GGTTGGTTTGGCGCAGCTTGC 17431 GGTTGGTTTGACGCAGCTTGC BaH25J08 AAGATGCACAGGAACATATTT 17432 AAGATGCACAAGAACATATTT BaH25J08 TGTAAGTAAAGAGATCGATAG 17433 TGTAAGTAAACAGATCGATAG baak3d11 ACATGCGAAACCACAAGAGAT 17434 ACATGCGAAATCACAAGAGAT baak3d11 ATACTATTAGAGTCTTCGGTA 17435 ATACTATTAGGGTCTTCGGTA baak3d11 CTTTGTAGCATTGCTGCCTAA 17436 CTTTGTAGCAATGCTGCCTAA baak3d11 ATGCAGATGAACAATTATAGT 17437 ATGCAGATGAGCAATTATAGT baak3d11 CTGCCTAACACATTAAAATTT 17438 CTGCCTAACAAATTAAAATTT baal12p08 AGTCTCAGAANGATGGGATGA 17439 AGTCTCAGAAGGATGGGATGA baal12p08 AGTGAGCTTTCATGTATCAGG  17440 AGTGAGCTTTNATGTATCAGG

TABLE 22-4 Haruna Nijo SEQ ID Clones H602 NO: bags39i20 GGTTGTCAGACTCGTGCACCT 17441 GGTTGTCAGATTCGTGCACCT bags39i20 TCTGGCACGGCCAAGGGGAAC 17442 TCTGGCACGGTCAAGGGGAAC bags39i20 AGGGGAACTCGAACTCTTGAT 17443 AGGGGAACTCTAACTCTTGAT kr15a0402 ATAGTAGAAATGCATGGTCAC 17444 ATAGTAGAAACGCATGGTCAC baal3c01 CATCAACACATTTCAGCAAGA 17445 CATCAACACAGTTCAGCAAGA baal3c01 CCACGGTGGGGACGCCGGGGT 17446 CCACGGTGGGAACGCCGGGGT baal3c01 CGTTGTTGTACGTGAACGCAC 17447 CGTTGTTGTAGGTGAACGCAC baak41i03 TCTCGGCACTTTTATGAGTTG 17448 TCTCGGCACTCTTATGAGTTG BaGS29M13 AGGAAGAAAAGGTAGAGATCT 17449 AGGAAGAAAAAGTAGAGATCT BaH24I06 TCATACTAATGCCCCACACGA 17452 TCATACTAATACCCCACACGA basd25c22 AGACTGCTTATTCATGCATTG 17454 AGACTGCTTACTCATGCATTG basd25c22 AATTCCGAACAGAGGGAGTAT 17455 AATTCCGAACGGAGGGAGTAT basd25c22 GGGTGGCTGCGTGTGACAACA 17456 GGGTGGCTGCATGTGACAACA basd25c22 AATGCCTCGAATTAGTTATGA 17457 AATGCCTCGACTTAGTTATGA basd25c22 TTTTTCCTTAACTATTATATC 17458 TTTTTCCTTATCTATTATATC basd25c22 TAACTATCTCCTCATCTGGGT 17459 TAACTATCTCATCATCTGGGT basd25c22 ATGACAGATGCAACCAAGCAT 17460 ATGACAGATGGAACCAAGCAT bah46p14 TATTCCAGGTAAAGCTACCCC 17463 TATTCCAGGTGAAGCTACCCC bags18d19 ATGGAACAGCACTGAGCATTT 17464 ATGGAACAGCGCTGAGCATTT bags18d19 ACAGAACTTTCTGCACAACAG 17465 ACAGAACTTTTTGCACAACAG bags18d19 GCAAAGAGATCAAATACAATA 17466 GCAAAGAGATAAAATACAATA bags18d19 TTACTTACAGCTCTCTTTCCA 17467 TTACTTACAGGTCTCTTTCCA bags18d19 GGACTTTATCGTTAACCGGGC 17468 GGACTTTATCATTAACCGGGC bags18d19 GATCTGAATATTAGCATACTC 17469 GATCTGAATACTAGCATACTC baak34j19 TTTTCTTCTTGTAGTGTGTTT 17470 TTTTCTTCTTTTAGTGTGTTT

TABLE 22-5 Haruna Nijo SEQ ID Clones H602 NO: baak34j19 TGAAGAAACATGAACAATATA 17471 TGAAGAAACAGGAACAATATA baak2m05 ACCTTTTTTAATGAAGCATAC 17475 ACCTTTTTTATTGAAGCATAC baak2m05 AGACACATAAAATGACGAGCT  17476 AGACACATAAGATGACGAGCT baak2m05 GAGTACATCGTAATAGACATA 17477 GAGTACATCGCAATAGACATA BaGS31B11 GCAAGGAATTGGAAACAGAAG  17479 GCAAGGAATTAGAAACAGAAG BaAL35M08 CAAGCCGCGCGCGAGCTAGCT 17480 CAAGCCGCGCACGAGCTAGCT BaAL35M08 CTGTATATGTCCGACCTGCAA 17481 CTGTATATGTGCGACCTGCAA BaH58J20 TGCCATGGTGTCTGAAATTCT 17483 TGCCATGGTGCCTGAAATTCT BaGS22B13 GTTCCTGCTTCTGACGAAGAG  17484 GTTCCTGCTTTTGACGAAGAG BaGS22B13 AACCCATATCCCACACACACC 17485 AACCCATATCTCACACACACC BaGS22B13 TAGGAGTAATTCACAACCCAT 17486 TAGGAGTAATCCACAACCCAT BaAK43M01 ATACACACTGAAAACCAGCGA  17487 GAACACACTGGAAACCAGCGA bah14i07 AGCCGGAAGATAATAACATGA 17488 AGCCGGAAGAAAATAACATGA bah14i07 TCAAGAATCGCCTTTGGGGGT 17489 TCAAGAATCGTCTTTGGGGGT bah14i07 GGTGTTTGGACCCAATACCAN  17490 GGTGTTTGGAACCAATACCAN baal9j16 CTGGCCAAAGAGTCTCCTCGG 17492 CTGGCCAAAGTGTCTCCTCGG baal9j16 CGGCGCGAAGGGATCGCGCTC 17493 CGGCGCGAAGCGATCGCGCTC baal9j16 TTGAACTATGCCCGGATGAGC  17494 TTGAACTATGACCGGATGAGC BaAK2O24 CCTTCCACTGCGAAGAAGTCA 17495 CCTTCCACTGTGAAGAAGTCA bags22j15 ATTGGCTTGCAGAATTCTCTC 17498 ATTGGCTTGCGGAATTCTCTC baak44a23 AAAATTCTATATTATGCTAGG 17499 AAAATTCTATGTTATGCTAGG baak32n04 GTGAAAAAGTGACCGCTTCTC  17500 GTGAAAAAGTCACCGCTTCTC basd21e22 CTCGTGTTTTTGCACTGCTCT 17501 CTCGTGTTTTGGCACTGCTCT basd21e22 CATGTACAACAAGAACGCGAC 17502 CATGTACAACGAGAACGCGAC baak42e22 GAAAATCTGCGGGCGTTCATC 17503 GAAAATCTGCAGGCGTTCATC

TABLE 22-6 Haruna Nijo SEQ ID Clones H602 NO: baake42e22 ACACCCACGTTTGAAAATCTG 17504 ACACCCACGTCTGAAAATCTG bast75e0610 TCGGAGAAGAGACGGAGGGCG 17505 TCGGAGAAGAAACGGAGGGCG baet16a1002 TCCGCTGAGCCGCGCTTCTAG 17506 TCCGCTGAGCGGCGCTTCTAG baak21i01 GGCCCTCCTCTGCCCTAGCCG 17508 GGCCCTCCTCCGCCCTAGCCG baak22o23 GAAAAGAGTCTGCACCTTATC  17510 GAAAAGAGTCCGCACCTTATC bah52h18 ATATATACGAACTCATGTAGC  17512 ATATATACGACCTCATGTAGC bah52h18 AGGCTTCTGCACCCCTTGCTG  17513 AGGCTTCTGCGCCCCTTGCTG bah52h18 CGTACAGAGAAGAAACAGACA  17514 CGTACAGAGAGGAAACAGACA baak33k20 ATTTTGACTACGGTAGCTTGG  17515 ATTTTGACTATGGTAGCTTGG baak33k20 TTTGAACTATCTGGGACTGGG 17516 TTTGAACTATGTGGGACTGGG baak33k20 AAAGCTTAGATAATAACTGGT 17517 AAAGCTTAGAGAATAACTGGT bags21f16 ATAGATGCTTCACCAGTGCAC 17518 ATAGATGCTTTACCAGTGCAC bags21f16 GTAGAACCTCATGAACATTTC  17519 GTAGAACCTCGTGAACATTTC bags34e05 CCGCGTCCACAGCGAAGTCCA 17520 CCGCGTCCACGGCGAAGTCCA bags34e05 GTCAACGCAGCTCTGGGCATC  17521 GTCAACGCAGTTCTGGGCATC bags19a02 GGCTTTTGCCACTATCAACTG 17523 GGCTTTTGCCGCTATCAACTG bags19a02 TCGGGTAGACGAAAAAACCCG 17524 TCGGGTAGACCAAAAAACCCG bags35k02 CCAACGGCACAATCATCCCTC  17525 CCAACGGCACGATCATCCCTC bah35a22 CTCTCCCTGCTGAAATGCCAT 17526 CTCTCCCTGCGGAAATGCCAT bah35a22 CAGCAACAGCTATAAAACAAT 17527 CAGCAACAGCCATAAAACAAT bags17h13 ACACTTGACCTTGGCTGCTGC 17528 ACACTTGACCGTGGCTGCTGC bah47n12 CGATGGGTACCGCAGCTTGAA 17529 CGATGGGTACGGCAGCTTGAA bah47n12 CTGCTTCTTCCGCTGGGGGCC 17530 CTGCTTCTTCTGCTGGGGGCC bah47n12 CTACACGTACACAAGCTGCAG  17531 CTACACGTACGCAAGCTGCAG baak12p11 AGAAGTAGTGAGTATGATTGT 17532 AGAAGTAGTGGGTATGATTGT

TABLE 22-7 Haruna Nijo SEQ ID Clones H602 NO: baet38B1004 ACCGAGACCCGACTCCACGGA 17533 ACCGAGACCCAACTCCACGGA baet38B1004 ATTTTCCCACACAGCCCTTTT 17534 ATTTTCCCACGCAGCCCTTTT BaGS13K12 TCGAGACGGCAGTCCACCAGC 17535 TCGAGACGGCGGTCCACCAGC baal25a05 GGTAGTTTATATGTGCAGCAT 17536 GGTAGTTTATGTGTGCAGCAT baal25a05 AGTGAACCAAATATCATAAAT 17537 AGTGAACCAAGTATCATAAAT baal25a05 TCATAAATGAGATGGTTTACG 17538 TCATAAATGAAATGGTTTACG baal25a05 TCGCCTTCTCAGTGTCCTTGT 17539 TCGCCTTCTCGGTGTCCTTGT BaAK24J12 TTCAGAGAATGAATGGATTAA 17540 TTCAGAGAATAAATGGATTAA BaAK24J12 CACCCACCTTTTATTTATTGT 17541 CACCCACCTTCTATTTATTGT basd1 4m22 GCAGTTATAAATTGCTACTGT 17542 GCAGTTATAATTTGCTACTGT basd1 4m22 AGCACTTCATAAGTGCTTCCA 17543 AGCACTTCATCAGTGCTTCCA bah17l24 TCCCCCACAGTCGTGTTTCTC 17544 TCCCCCACAGGCGTGTTTCTC bah17l24 AAAGGTAAGTAAACATAAACT 17545 AAAGGTAAGTGAACATAAACT baal29j09 AGCCCTATGTAAAAACATTTC 17546 AGCCCTATGTGAAAACATTTC baal29j09 TAGGGCACCAAACAAACTAGA 17547 TAGGGCACCAGACAAACTAGA baal29j09 TATGTACACATTTTTGCACAG 17548 TATGTACACAATTTTGCACAG baal29j09 TCATTCAGAGTCGTATATGTA 17549 TCATTCAGAGCCGTATATGTA baal29j09 AATGGTAATTACTCCAGAAGA 17550 AATGGTAATTGCTCCAGAAGA bah16d09 CATGTTGGATAGCTGCAACAC 17551 CATGTTGGATTGCTGCAACAC bah16d09 TTGCGCTCTCGGCAAGGCATA 17552 TTGCGCTCTCTGCAAGGCATA bah60d03 GGGCAACAAGATCGCCTGCAT 17553 GGGCAACAAGGTCGCCTGCAT bah60d03 ACAAATAATTTTGACAGTTCA 17554 ACAAATAATTCTGACAGTTCA bah60d03 TTATGGGCTCCGCGGCTTCTG 17555 TTATGGGCTCTGCGGCTTCTG bah45i13 GCAAAAGCAGCTTGAAGTATA 17556 GCAAAAGCAGTTTGAAGTATA bah11h03 GCGCCAAGATCAGGCGGCTGA 17557 GCGCCAAGATAAGGCGGCTGA

TABLE 22-8 Haruna Nijo SEQ ID Clones H602 NO: bah47h17 CATCCACCATTACAAGACAAG 17558 CATCCACCATAACAAGACAAG bah47h17 CACTCCCTCAGGAACTCAACA 17559 CACTCCCTCAAGAACTCAACA bags35d02 ATCAGTTTATATGCCTCGTGT 17560 ATCAGTTTATTTGCCTCGTGT bags35d02 AATAAAATTGCCTTATTTTTG  17561 AATAAAATTGTCTTATTTTTG bags35d02 GATGTAGAAGTTGGAGAGCAT 17562 GATGTAGAAGCTGGAGAGCAT baak14c12 TCAAGTTCTCGGTGTCGTAGA  17564 TCAAGTTCTCAGTGTCGTAGA baak34c01 GCATGTCGTCATTGTCCCCAT 17565 GCATGTCGTCGTTGTCCCCAT bah35a11 AGTTATATTAAGGTTGAGACA  17566 AGTTATATTATGGTTGAGACA bah35a11 ACCACACAAGGCTCAGATCTT  17567 ACCACACAAGCCTCAGATCTT BaAK30M16 GTTTACTGCCGAAATTAGCAG 17568 GTTTACTGCCAAAATTAGCAG BaAL2G20 GTAAATATGCAATAATGCTGT  17571 GTAAATATGCTATAATGCTGT BaAL2G20 GCTAATTCAGGTTCACTGTAG  17572 GCTAATTCAGATTCACTGTAG bah37f01 AAGCTGGCGCCTGTACTATGT 17576 AAGCTGGCGCATGTACTATGT bah37f01 GACGTGGCCGTATATTCTTCT 17577 GACGTGGCCGCATATTCTTCT bags29m05 CAAACGTCACACCCTTCTTGT 17578 CAAACGTCACGCCCTTCTTGT baet34A0501 TGGTAGATGCTCGCGAATCCT  17580 TGGTAGATGCCCGCGAATCCT BaH30E13 GCTGGCCTGGCCGTTTTGTCG 17584 GCTGGCCTGGTCGTTTTGTCG BaH30E13 CGAGCATATGTTTGATTTTTG 17585 CGAGCATATGGTTGATTTTTG bags4e23 GTCCAGTTTGTTGGGGGGAGA 17586 GTCCAGTTTGCTGGGGGGAGA baak45b03 TGGCCGTGTAGAAGGCGTCCA  17587 TGGCCGTGTACAAGGCGTCCA baak45b03 TATACGCGCGTGCGTACGTAC 17588 TATACGCGCGCGCGTACGTAC baak45b03 AGGCGTCCACGGTGGTGTCGA 17589 AGGCGTCCACTGTGGTGTCGA bah12o12 GATCATCAGTTGGTCTAACAC 17590 GATCATCAGTCGGTCTAACAC bah12o12 TAGAAATGAAGTCAAGTGCTC 17591 TAGAAATGAAATCAAGTGCTC bah12o12 GAATCGGAACCGTTATAATCA 17592 GAATCGGAACTGTTATAATCA

TABLE 22-9 Haruna Nijo SEQ ID Clones H602 NO: baal3e14 GTCGGAGCATTGCTCACATAT  17594 GTCGGAGCATCGCTCACATAT basd1 j14 CAGAATAAAACGGAGGAACAT 17598 CAGAATAAAATGGAGGAACAT basd1 j14 TTTGGCCATAAAGCATGGCAC  17599 TTTGGCCATAGAGCATGGCAC bags35b18 GAGGAGGTGCTGGCTTTCTTA  17600 GAGGAGGTGCCGGCTTTCTTA baal41i11 AACCAAATCAAATCGGTTACA 17601 AACCAAATCAGATCGGTTACA baal41i11 CATCTCTTGTGTACGTACCAT  17602 CATCTCTTGTTTACGTACCAT baal41i11 GCCAGGCTTACATATTTTGGA  17603 GCCAGGCTTAGATATTTTGGA baal41i11 ATGTGATTGGGGCTCAACTGA  17604 ATGTGATTGGTGCTCAACTGA bags13e23 AACATAATTTTCAATCTGTTA  17605 AACATAATTTCCAATCTGTTA bags13e23 GGCCTGCACATTGGGGAAAAT  17606 GGCCTGCACAGTGGGGAAAAT bah20d03 TCGCCCTTGGGGGAGACAGCG  17607 TCGCCCTTGGAGGAGACAGCG baet19f0212 AGCAGAAAGGAGCAGCTCAAC  17608 AGCAGAAAGGGGCAGCTCAAC basd12j05 AAGAAGAACAACACAGAAGTT  17609 AAGAAGAACAGCACAGAAGTT basd24d17 CCTAAGCAGTAGGTGCATTCT 17610 CCTAAGCAGTCGGTGCATTCT basd24d17 GACACAACAGAGGGCTCCTGG  17611 GACACAACAGGGGGCTCCTGG basd24d17 AGGCGTTGAGGACGGCTGATA  17612 AGGCGTTGAGAACGGCTGATA bags24k10 AAAAAAACACATGTACCTTAT 17613 AAAAAAACACGTGTACCTTAT baal9e05 GTAAAATGCCAGCGCGTCTAC  17614 GTAAAATGCCGGCGCGTCTAC baal9e05 TGCCCCACCACTTGCGCTTGG  17615 TGCCCCACCATTTGCGCTTGG baal9e05 TGCCACCGGCACTGCCGCCGC  17616 TGCCACCGGCCCTGCCGCCGC bags33m23 AATACAAGTAGGATCATCCTA 17617 AATACAAGTACGATCATCCTA bags33m23 TGTCCTCTCTCCTGACAAAAC  17618 TGTCCTCTCTTCTGACAAAAC bags33m23 AATAAATGAATGAAGACAACA  17619 AATAAATGAACGAAGACAACA bah46g14 AAATCAATGAGCAGGTAGAAT  17620 AAATCAATGAACAGGTAGAAT bah46g14 AGCTCAGATCGAAACCGGCTT 17621 AGCTCAGATCAAAACCGGCTT

TABLE 22-10 Haruna Nijo SEQ ID Clones H602 NO: bags33h05 GCTGGAAATTTTTCCACGGGG 17622 GCTGGAAATTGTTCCACGGGG bags33h05 TGGCAATGCTATGCCGGCTCA 17623 TGGCAATGCTTTGCCGGCTCA baak24h12 ATATGCTACCACTAAGCTGCA 17624 ATATGCTACCCCTAAGCTGCA baak24h12 CACACTAGATGAGATGGAGGG 17625 CACACTAGATAAGATGGAGGG bah50n19 ACGGCGCCGATAGGGCGGAGA 17626 ACGGCGCCGAGAGGGCGGAGA baak31o05 GATCGAGGTAGCCGACGGAAT 17627 GATCGAGGTATCCGACGGAAT baak3lo05 ACGCTGTAAATTGGCTACCGG 17628 ACGCTGTAAACTGGCTACCGG bags39l14 CCCCTGCGTGATCACCGTGTC 17629 CCCCTGCGTGGTCACCGTGTC bags39114 TCAATATTTGAAGGTGCTTGA 17630 TCAATATTTGCAGGTGCTTGA bags39114 TGGATCTACCATCAATATTTG 17631 TGGATCTACCTTCAATATTTG bags39114 AATAAGTTGAGAGACTGGATC 17632 AATAAGTTGAAAGACTGGATC bags39114 CGTGCATGTTCGCCTGCACAG 17633 CGTGCATGTTTGCCTGCACAG bags39114 GTTTGCAAACAAAAAGAAGCT 17634 GTTTGCAAACGAAAAGAAGCT BaGS27C22 GTTACAACTCGGTTCAATTCC 17635 GTTACAACTCAGTTCAATTCC bags20o24 TGTCAACTCATCTTAGTGTCG 17636 TGTCAACTCAACTTAGTGTCG bags20o24 TCTACATAGAATGTACTAGCT 17637 TCTACATAGACTGTACTAGCT bags20o24 CAATCGACAGTGGCTCCAGGG 17638 CAATCGACAGCGGCTCCAGGG bast42c0806 CCATATAAAAGGGTTCAGCTA 17651 CCATATAAAAAGGTTCAGCTA baak26c05 CAGTTCGATGCCGATTGCAAA 17652 CAGTTCGATGTCGATTGCAAA baak26c05 ATGCGTTCAACATTTAGCCTG 17653 ATGCGTTCAAAATTTAGCCTG bags19a16 GGTGTTGACATTACAACAAAG 17654 GGTGTTGACACTACAACAAAG bags19a16 TCAAAGCTACGCACGACTGCG 17655 TCAAAGCTACCCACGACTGCG bags19a16 GAGTGATTCACATGTGATTGC 17656 GAGTGATTCAGATGTGATTGC kr11f1212 CACACTCGTTCACACACGACA 17657 CACACTCGTTAACACACGACA baak35l20 GGATGCAGTCAGCGAACTTGC 17660 GGATGCAGTCGGCGAACTTGC

TABLE 22- 11 Haruna Nijo SEQ ID Clones H602 NO: bah57m07 CTCGTGAAGAATGACACAGGC  17661 CTCGTGAAGAGTGACACAGGC bah60k11 TCCATGAGGCCGAACCTAACT 17662 TCCATGAGGCTGAACCTAACT bah60k11 ATAAGAAATCGTCAGAAACAG  17663 ATAAGAAATCATCAGAAACAG bah60k11 AGAAACAGTGAGAAAACCTAG  17664 AGAAACAGTGGGAAAACCTAG basd20o09 ATATAAGTTCACTAATTTTGC  17665 ATATAAGTTCTCTAATTTTGC basd20o09 TCTAAACGATTATTACCACCA  17666 TCTAAACGATCATTACCACCA basd24i22 GCCTACACTTCACTGATGATT  17667 GCCTACACTTTACTGATGATT basd24i22 ATGACTCACAATGTGACAGCA  17668 ATGACTCACATTGTGACAGCA bags30g20 CGGACTGAAACGAGTGAACAA  17669 CGGACTGAAATGAGTGAACAA bags30g20 TAAGAAAGATGACATGAAAAT  17670 TAAGAAAGATCACATGAAAAT bags1 m11 GTTCATTCTCGGCCAAGGTCA  17671 GTTCATTCTCAGCCAAGGTCA bags23g20 GACAGACGCCGATGGACATGC 17672 GACAGACGCCAATGGACATGC bags23g20 GTATTTCCGGGAATTCTGCAG  17673 GTATTTCCGGAAATTCTGCAG bags15b05 GCACGCATGTGTCCTTGAATT  17674 GCACGCATGTATCCTTGAATT bags15b05 CCTCCGACTAGTCGATCTTAT  17675 CCTCCGACTACTCGATCTTAT bags15b05 GCTGGCGGCGGCGCCGTTGCC  17676 GCTGGCGGCGCCGCCGTTGCC BaGS24P05 TCACTCGTAATGACATGATCA  17677 TCACTCGTAACGACATGATCA bags10g06 ACGTCCTGTGATTTGCGTGCC  17678 ACGTCCTGTGCTTTGCGTGCC bah32o04 GCGACCGCCATCTCAGGCTCG  17679 GCGACCGCCACCTCAGGCTCG bah32o04 CGACGGGAGAGTGGCCAGCCA  17680 CGACGGGAGACTGGCCAGCCA bags32p08 CACCACTGCTCGATTGTTCAT  17681 CACCACTGCTTGATTGTTCAT BaGS37L06 TCTCGGCCTCGGTGAACTCCA  17685 TCTCGGCCTCAGTGAACTCCA BaGS37L06 AAAGCACAATGCCACATAAAT 17686 AAAGCACAATACCACATAAAT BaGS37L06 CATTCATGTTGCTCTCGGCCT  17687 CATTCATGTTACTCTCGGCCT bags3h12 AAGAAAAATGTGAGGAGAGAA  17689 AAGAAAAATGAGAGGAGAGAA

TABLE 22-12 Haruna Nijo SEQ ID Clones H602 NO: bags3h12 ACAAGTCAAGCAAGCCGTACG 17690 ACAAGTCAAGTAAGCCGTACG bags1e21 CACCATTGTTTGGCAATTTAC 17691 CACCATTGTTGGGCCATTTAC bags1e21 ATTGTTTGGCAATTTACAGCA 17692 ATTGTTGGGCCATTTACAGCA baak27m21 CCAACCAGCAGAAAACAGTTT 17693 CCAACCAGCACAAAACAGTTT bah35b05 GCACGGACCAACATCAAAGGT 17694 GCACGGACCAGCATCAAAGGT basd21h11 ATCATAAGAACGATTTGGAGA 17695 ATCATAAGAATGATTTGGAGA BaH32E20 TCGTGCCCCATCTCCCTCGTT 17696 TCGTGCCCCACCTCCCTCGTT BaH32E20 TGTCTGGGCAGGTGTGGAGCG 17697 TGTCTGGGCAAGTGTGGAGCG bags22a20 GAAGCTACTCGTCAGTTAGGA 17698 GAAGCTACTCATCAGTTAGGA bags22a20 AATCCGCATGATGCAGCGATG 17699 AATCCGCATGGTGCAGCGATG bags29l04 CACCTGATACAATGAAGATGT 17701 CACCTGATACGATGAAGATGT bags22g16 AAAACTCGTCGGCGCTGCTGT 17702 AAAACTCGTCAGCGCTGCTGT bags22g16 CCACACTTGTAATTAGCAACC 17703 CCACACTTGTGATTAGCAACC bah61h20 CGTTCATAACACATCACCATT 17704 CGTTCATAACGCATCACCATT bah61h20 CGTGCCTAACGACCTGAAACG 17705 CGTGCCTAACAACCTGAAACG bah61h20 TCTGAATGATGGTGTGAAAGA 17706 TCTGAATGATAGTGTGAAAGA bah15p01 CTGCATTTGGGTTGGITCCAG 17707 CTGCATTTGGATTGGTTCCAG baak41p03 TCTGGACTTGTGACGCCACGA 17708 TCTGGACTTGCGACGCCACGA BaSD12L06 ACACAGTTTAGTAATACTGTA  17709 ACACAGTTTAATAATACTGTA BaSD12L06 TTCTGTACTACAAGAGATGAC 17710 TTCTGTACTAAAAGAGATGAC BaSD23P07 CCCTGAAGTCACTGCCCTTGA 17711 CCCTGAAGTCGCTGCCCTTGA BaGS7J05 GGGATGAGTCCAGCGACTCGA 17712 GGGATGAGTCGAGCGACTCGA BaGS7J05 AGTCACACTTTCAGGGTCGAC 17713 AGTCACACTTCCAGGGTCGAC BaGS7J05 TTTGAGACGCTCGACTAGTCG 17714 TTTGAGACGCCCGACTAGTCG BaGS7J05 TGAAATTATCGTGAAACGGAA 17715 TGAAATTATCATGAAACGGAA

TABLE 22-13 Haruna Nijo SEQ ID Clones H602 NO: bah29b06 AGAGGAAGCCGTCCCTGCGAT 17716 AGAGGAAGCCATCCCTGCGAT bags31n17 TAAACACAGACGCAGCTTAGC 17717 TAAACACAGATGCAGCTTAGC bags31n17 CATGCTGATCCATACTGCTCT 17718 CATGCTGATCAATACTGCTCT bags31n17 TTGCCAGTAACTACAGCAGTT 17719 TTGCCAGTAATTACAGCAGTT bags31n17 TACTTTGAGATGCAGAAAGCA 17720 TACTTTGAGACGCAGAAAGCA bags31n17 ATGAACATACGAAAATCTAAC 17721 ATGAACATACTAAAATCTAAC bags31n17 ACAAGTAGAGCCAAAAGGTGA 17722 ACAAGTAGAGACAAAAGGTGA BaGS13F08 CAAAACAATTTAGCCACAAAA 17723 CAAAACAATTCAGCCACAAAA bags39d12 ATGTCCAAAAAGAAAGTCAGC 17727 ATGTCCAAAAGGAAAGTCAGC bags39d12 TCATGGTACAGAAATCTTACA 17728 TCATCGTACATAAATCTTACA bags39d12 TTACTTGTTTTGTTGATAGAC 17729 TTACTTGTTTCGTTGATAGAC BaAK20B19 ACATCTTGCCATCGAACTTGT 17731 ACATCTTGCCGTCGAACTTGT BaAK20B19 TCCGCACGGCGACCGCCCTGC 17732 TCCGCACGGCCACCGCCCTGC bags15j15 TCTTCCTCGTCGTCGTCGAGA 17733 TCTTCCTCGTTGTCGTCGAGA bags15j15 GAAGTACTTGTTCCTCTCGGC 17734 GAAGTACTTGCTCCTCTCGGC BaGS22A21 ACATATCGCACATATCTGAGC 17736 ACATATCGCATATATCTGAGC BaGS22A21 GGCAACCATCATTAGGCACAA 17737 GGCAACCATCGTTAGGCACAA BaGS22A21 CAAAGGCAGCGATCTTCTTCC 17738 CAAAGGCAGCAATCTTCTTCC BaGS22A21 TCTTCTTCCCATTCTTCACCA 17739 TCTTCTTCCCGTTCTTCACCA BaGS22A21 ACTTCAGGTTGTATAGGACAT 17740 ACTTCAGGTTATATAGGACAT BaGS22A21 CCTTAACAGTCCATTCCATCC 17741 CCTTAACAGTGCATTCCATCC baet45e0410 CAAGCGGTAAGCATAACTACA 17742 CAAGCGGTAATCATAACTACA bags22c14 AGGTTCATCAGTTCCCTTTCC 17743 AGGTTCATCAATTCCCTTTCC bags22c14 TAATCCACCGCCCCTCAATCT 17744 TAATCCACCGACCCTCAATCT bah23k12 GCTAGGACTGTAAAATAAACG 17745 GCTAGGACTGCAAAATAAACG

TABLE 22-14 Haruna Nijo SEQ ID Clones H602 NO: bah15o13 ATCCAAATTACAACCACTTCC 17746 ATCCAAATTAGAACCACTTCC kr24f0412 GAACAGAAGATTGCCATTAAC 17747 GAACAGAAGAATGCCATTAAC kr24f0412 TAGCTGGCGGGTAACAGTTTC 17748 TAGCTGGCGGATAACAGTTTC bags28o21 TCCTCTTCGAACTGTAGGGGA 17749 TCCTCTTCGAGCTGTAGGGGA bags28o21 CGCCCTTGCCCAGACCCTTGC 17750 CGCCCTTGCCGAGACCCTTGC bags28o21 AGATCTTGAGCACGCCGCGGG 17751 AGATCTTGAGGACGCCGCGGG baal15n19 CAACGGAGGCAGCGCACTGTC 17752 CAACGGAGGCGGCGCACTGTC baal15n19 CCGCGATGGCAAGGGCGGCGC 17753 CCGCGATGGCGAGGGCGGCGC bags32j03 CAAATTGATCGCAGACTCCAC 17755 CAAATTGATCACAGACTCCAC bags32j03 ACTACTAAGAGAGTCCAGCTG 17756 ACTACTAAGAAAGTCCAGCTG bags32j03 CAGACCAGACAACAGAGATGA 17757 CAGACCAGACCACAGAGATGA bags20e14 ACCTGTCCTGTTTCAGCGCGT 17758 ACCTGTCCTGCTTCAGCGCGT basd27n01 AACCCCGCCAGTTGTATAAGA 17760 AACCCCGCCACTTGTATAAGA basd27n01 TATCAGGGATCCAGGAGGGGA 17761 TATCAGGGATTCAGGAGGGGA basd27n01 TTCACGAGTGCACACTACAAT 17762 TTCACGAGTGTACACTACAAT basd27n01 CAGGAGGGGAGGAGCGATCGC 17763 CAGGAGGGGACGAGCGATCGC bags6m19 GAATAACGACCTACAAATCCA 17764 GAATAACGACGTACAAATCGA bags6m19 CCTACAAATCCAGTATACTAG 17765 CGTACAAATCGAGTATACTAG baak13e03 CATAGGGCTCCTCGTTATCAA 17766 CATAGGGCTCGTCGTTATCAA bah23i02 AGAATGGCGACATTAGAAATA 17774 AGAATGGCGAGATTAGAAATA bah23i02 CCTGGCCGCGCGACCTTTATA 17775 CCTGGCCGCGGGACCTTTATA baak15h22 ATTTTGATTCGCACCTGCACT 17776 ATTTTGATTCACACCTGCACT baak15h22 CAGAGTATACTAGACGTAGTA 17777 CAGAGTATACCAGACGTAGTA baak15h22 TATTGTCGACCCTCACACAGC 17778 TATTGTCGACACTCACACAGC bags32b03 AATACCAAGTCACCCGTTGTG 17779 AATACCAAGTAACCCGTTGTG

TABLE 22-15 Haruna Nijo SEQ Clones H602 ID NO: baak30c15 ATGAATGGCCGGGCACAATGC 17780 ATGAATGGCCAGGCAGAATGC baak30c15 TGGCCGGGCACAATGCATATT 17781 TGGCCAGGCAGAATGCATATT BaGS22A05 AGCTAACTAGGGCCAACGCCT 17782 AGCTAACTAGCGCCAACGCCT BaGS22A05 CAAGGCGCCCGTTCTTGACCT 17783 CAAGGCGCCCATTCTTGACCT BaGS22A05 CGAAGCTGCCGCCAGGGTAGA 17784 CGAAGCTGCCACCAGGGTAGA bah16m01 GTTTTGGCTCTCCTAGCAAGG 17785 GTTTTGGCTCGCCTAGCAAGG bags37d12 CTCGGACCCCCGGAGCAGCAG 17787 CTCGGACCCCTGGAGCAGCAG baal37n24 CTCCCTCCTCTGTTCCTGATT 17788 CTCCCTCCTCCGTTCCTGATT baal37n24 CCATGAGGCCGATGATCCACC 17789 CCATGAGGCCAATGATCCACC baal37n24 TTGCCGTGCTCTGTATCGGAC 17790 TTGCCGTGCTTTGTATCGGAC BaH28B09 CAACAAATACAAAAGTGAGTT 17791 CAACAAATACGAAAGTGAGTT BaH28B09 AATCGGCCGGGGACCATCAGA 17792 AATCGGCCGGAGACCATCAGA BaH28B09 ACGATGCAGCGGACACAAGCT 17793 ACGATGCAGCAGACACAAGCT BaH28B09 TTGTCTTTTATAATAAGCATT 17794 TTGTCTTTTAAAATAAGCATT BaH28B09 TTTTATTGACGGTGCAAAACA 17795 TTTTATTGACAGTGCAAAACA BaH28B09 AAACTCTGACGTGGGATAGAA 17796 AAACTCTGACATGGGATAGAA bah47h08 ATGTGTTGATCACAGCTGCTC 17797 ATGTGTTGATTACAGCTGCTC bah57n07 TGGTGCAGACAGGCGCGGTGA 17798 TGGTGCAGACGGGCGCGGTGA baak38o02 CCACGACGTAGGTACGTCGTT 17799 CCACGACGTACGTACGTCGTT baak20f16 AGTCATTTATTTGGAATGAAG 17800 AGTCATTTATCTGGAATGAAG baak20f16 ACTCAACTAACTTTTAGGTGG 17801 ACTCAACTAATTTTTAGGTGG baak28n19 CGAAGCTGCCGCCAGGGTAGA 17802 CGAAGCTGCCACCAGGGTAGA baak28n19 CGAGGCCACCCTCGCTGAAGA 17803 CGAGGCCACCGTCGCTGAAGA bah59j07 AGCTAGTTCAGACATCGTCCT 17804 AGCTAGTTCACACATCGTCCT bah59j07 GGAATCTTGGATAGTTGGAGC 17805 GGAATCTTGGGTAGTTGGAGC

TABLE 22-16 Haruna Nijo SEQ Clones H602 ID NO: bah59j07 TCCATGACGATTCCACACACC 17806 TCCATGACGAATCCACACACC bah59j07 GGTACTGGCAACATCCATGAC 17807 GGTACTGGCAGCATCCATGAC bah59j07 TCCTCTTGCTGTCTTACAGTC 17808 TCCTCTTGCTATCTTACAGTC bah59j07 AGACAAAGAAGATTGCAAGTC 17809 AGACAAAGAAAATTGCAAGTC bah59j07 CAGGTCGTTCGGGGGAAGCAG 17810 CAGGTCGTTCTGGGGAAGCAG bah59j07 CTGCTTGAAACATCACCATAA 17811 CTGCTTGAAATATCACCATAA bah59j07 CAAATGCCTTGTGTGCACTGC 17812 CAAATGCCTTATCTGCACTGC bah44j20 AAGGTCTTGCAACTGAAGGCG 17813 AAGGTCTTGCGACTGAAGGCG baet39b0303 CCATCTCCGCATTGGCCCAGT 17815 CCATCTCCGCCTTGGCCCAGT baet39b0303 GCTTTCTTGGCAGGGTAAGGA 17816 GCTTTCTTGGTAGGGTAAGGA baet39b0303 GCTCGCCGTCGAGCACGCTGC 17817 CCTCGCCGTCCAGCACGCTGC baak27d19 ACGAGTGGTCGTCCCAGCCAG 17818 ACGAGTGGTCTTCCCAGCCAG baak27d19 TTAACAGCCGGCGGCTGAACG 17819 TTAACAGCCGACGGCTGAACG basd25g01 TCCCTACCTTCCCTGGCCTCC 17820 TCCCTACCTTACCTGGCCTCC basd25g01 TCCCGGAGCTCGCGTTCGCGT 17821 TCCCGGAGCTAGCGTTCGCGT baal34k17 TCTTTCGGAGCTGCTACTCTA 17822 TCTTTCGGAGATGCTACTCTA baal34k17 AGTTTCGGGGTAAATTTATCC 17823 AGTTTCGGGGAAAATTTATCC basd21i17 CCTCCCCGCACCGCAGTGTGG 17824 CCTCCCCGCATCGCAGTGTGG basd21i17 GGCAGCATCTCACGAAACACC 17825 GGCAGCATCTGACGAAACACC bagsl5o12 GAAAAGAGTGAAAACATACAA 17826 GAAAAGAGTGGAAACATACAA baak44n10 GTGATGGCAATGAATTTTATG 17828 GTGATGGCAAGGAATTTTATG baak44n10 GTGACCTCGTCGAAACTTGGT 17829 GTGACCTCGTTGAAACTTGGT baak21e08 TGCGGTACACTGCGCTCAGCC 17830 TGCGGTACACCGCGCTCAGCC baak41i21 CGAGCCGACAGTGCACACCGC 17832 CGAGCCGACACTGCACACCGC BaGS19C07 CCGCGCCTCTCGGGTCACCGG 17833 CCGCGCCTCTTGGGTCACCGG

TABLE 22-17 Haruna Nijo SEQ Clones H602 ID NO: BaGS19C07 TGAAAAATGTCATATCATTCA 17834 TGAAAAATGTTATATCATTCA BaGS19C07 GACAGGATGAGCAACTTGCCT 17835 GACAGGATGAACAACTTGCCT BaGS19C07 CTCGATTGCCAATGTATATGA 17836 CTCGATTGCCGATGTATATGA BaGS19C07 CTTTCCTGATCATGGAGCAAT 17837 CTTTCCTGATAATGGAGCAAT BaGS19C07 CTGGTATTGGAGGCAATGGGA 17838 CTGGTATTGGCGGCAATGGGA BaGS15O08 CCCTGCCGGCAATCTCACCGA 17839 CCCTGCCGGCGATCTCACCGA BaGS15O08 CAGACATCTCATCTGTCCAGG 17840 CAGACATCTCGTCTGTCCAGG baak40p22 ATATGGCACCCATACAACCCT 17841 ATATGGCACCGATACAACCCT baal40n03 TAGTAATTGTAATGTAGTAGG 17843 TAGTAATTGTTATGTAGTAGG baal40n03 GGCCTCGGCCACCATAGAACC 17844 GGCCTCGGCCGCCATAGAACC bags23b08 CAGATCACTTGCAAATGTGGT 17845 CAGATCACTTACAAATGTGGT bags23b08 GTTTGTGTATGAGAAGAAGAG 17846 GTTTGTGTATCAGAAGAAGAG bags23b08 AGTCGTTGTCCATCTGGTCAT 17847 AGTCGTTGTCTATCTGGTCAT BaGS39L18 TGGCCTTCAGCTCAGGCTTCA 17848 TGGCCTTCAGTTCAGGCTTCA BaGS39L18 CCAGGGCATACTCCTTCCTAC 17849 CCAGGGCATAGTCCTTCCTAC BaGS39L18 CCTCCAAGTCAGAAGAGATGA 17850 CCTCCAAGTCGGAAGAGATGA bah18d15 AGAAGAGATCATTCGCCGCCA 17853 AGAAGAGATCGTTCGCCGCCA bastl04f0911 TCTTGCACGAGCAATGAGGTG 17854 TCTTGCACGACCAATGAGGTG BaGS31E03 CCTTGAAAACTGACGAGTAGT 17855 CCTTGAAAACCGACGAGTAGT BaGS19J21 ACTGCGGCACACTCCAGGTCT 17856 ACTGCGGCACGCTCCAGGTCT BaGS19J21 GAGTGCCAAGCTTCCATAGCA 17857 GAGTGCCAAGTTTCCATAGCA BaGS19J21 GGCTTGGAGTTGGAGGTACTG 17858 GGCTTGGAGTCGGAGGTACTG bags39p08 GAATCAGATGAACAAGACAGT 17859 GAATCAGATGCACAAGACAGT BaH56B06 TCAGTCCTCATCAAGTAGCAT 17860 TCAGTCCTCACCAAGTAGCAT baaL13m24 ACAACACAAGCAAATGAAGGG 17861 ACAACACAAGGAAATGAAGGG

TABLE 22-18 Haruna Nijo SEQ Clones H602 ID NO: baal13m24 GATCAGGATCGTCACTTTTCC 17862 GATCAGGATCATCACTTTTCC baal13m24 ATACGCTCTTTATATGCTACA 17863 ATACGCTCTTAATATGCTACA baal13m24 ATGAAGGGGGTAAAACAGGTT 17864 ATGAAGGGGGCAAAACAGGTT bags15h14 AGGAGGAGGCGTCGTCAGAAA 17865 AGGAGGAGGCATCGTCAGAAA baal34b14 ACTGAGAGTCAACCACTGTAT 17868 ACTGAGAGTCGACCACTGTAT baal34b14 TGTCGATGCACAAGTTGCGAC 17869 TGTCGATGCAGAAGTTGCGAC baal34b14 AATAGTCTGTCTCAGTCTCAC 17870 AATAGTCTGTTTCAGTCTCAC baal34b14 CAAACCAAGATAGCATCAGTC 17871 CAAACCAAGAAAGCATCAGTC bast58C1206 TTCTTCTTCTTGGGCAACGTA 17874 TTCTTCTTCTAGGGCAACGTA bast58C1206 TTTAGGTAAACTGAACCTCAA 17875 TTTAGGTAAATTGAACCTCAA bastl20B0404 AAAAAAAAAAAAAAAAAAAAA 17877 AAAAAAAAAAGAAAAAAAAAA bastl20B0404 AAGAGCTGACCGTGGAAACCA 17878 AAGAGCTGACTGTGGAAACCA bastl20B0404 AAAAAAAAAAGAAAAAAAAAA 17879 AAAAAAAAAAAAAAAAAAAAA BaH15P22 TGGGGGCACCGACCTTTCGGA 17881 TGGGGGCACCAACCTTTCGGA BaGS17I22 AAACAAGTGCAGACCATGGCC 17882 AAACAAGTGCCGACCATGGCC BaGS17I22 GCATGTACTCATTGGAGGAAC 17883 GCATGTACTCGTTGGAGGAAC BaGS17I22 CTCAACATCAAACATACTGCT 17884 CTCAACATCATACATACTGCT bagsl5h01 CAGGAGGAATGCTAAACTGCT 17885 CAGGAGGAATACTAAACTGCT bagsl5h01 GCTAGAATGTTCTAGATCAGT 17886 GCTAGAATGTACTAGATCAGT bags7p13 TGGTCACTCAGGCAGAAAGGA 17887 TGGTCACTCAAGCAGAAAGGA bags29h13 TGGGGGCACCAACCTTTCGGA 17888 TGGGGGCACCGACCTTTCGGA bags29h13 TGTGAGGGACAAGTCAATACT 17889 TGTGAGGGACGAGTCAATACT bah47b01 AGTAGATGCAAGGGTTCGAAG 17890 AGTAGATGCAGGGGTTCGAAG bah47b01 TGAGCACATATATGTAGTAGA 17891 TGAGCACATACATGTAGTAGA bags32m15 TATGATTGACACTACGACTTT 17893 TATGATTGACGCTACGACTTT

TABLE 22-19 Haruna Nijo SEQ Clones H602 ID NO: bags32m15 ATGACCATCCAGTGGGGTAGT 17894 ATGACCATCCGGTGGGGTAGT bags32m15 TCATGCTTGAAACGGTGCAGA 17895 TCATGCTTGAGACGGTGCAGA bags32m15 GACTTCAAGGGGGTTCCACAA 17896 GACTTCAAGGTGGTTCCACAA baal27m11 ACAGTATATCGCACGAGAAAA 17897 ACAGTATATCACACGAGAAAA baal27m11 GCTGGCCTGTGGCACCATTCG 17898 GCTGGCCTGTCGCACCATTCG baal27m11 TGTCTGCAGAGTGATTCTGCA 17899 TGTCTGCAGAATGATTCTCCA basdl2k01 GAGTGAACAGCCTTGGAAAGC 17900 GAGTGAAGAGTCTTGGAAAGG bags1b01 TCGCCAGCTCGCCGACACCAC 17901 TCGCCAGCTCACCGACACCAC bags21e21 GGTGCACACGGGACACATAAC 17904 GGTGCACACGAGACACATAAC baes21e21 TCTGTGGCTTCGCAGGATCTT 17905 TCTGTGGCTTGGCAGGATCTT bags21e21 TCATCATGTCAGCGCCAGATA 17906 TCATCATGTCCGCGCCAGATA bags21e21 ACATGTTTGCGGTGTTGAGGT 17907 ACATGTTTGCAGTGTTGAGGT baes21e21 TGAAGTGGTCTCGGGGGTGCA 17908 TGAAGTGGTCGCGGGGGTGCA baet02b0503 CCACCAGCACTCCGGCACCTG 17909 CCACCAGCACGCCGGCACCTG baet02b0503 GGCCGTATGGATCCATTGGCG 17910 GGCCGTATGGCTCCATTGGCG bah29l05 GATTTCTTGAGTTGTAATCCG 17911 GATTTCTTGAATTGTAATCCG bah29l05 GCAACATCCGCCTAGATTTCT 17912 GCAACATCCGACTAGATTTCT bah29l05 TGTAGTCCCCATGCAACTTCC 17913 TGTAGTCCCCGTGCAACTTCC bah29l05 TTTTATTAAAGAAATAGAAAT 17914 TTTTATTAAAAAAATAGAAAT baet43H1016 GACAGGGTCGGCAATGTGGTC 17915 GACAGGGTCGACAATGTGGTC baet43H1016 TAATATACATCGTTGGTGTTA 17916 TAATATACATAGTTGGTGTTA baak20b06 CACACTCATCGATCCATTCGC 17921 CACACTCATCAATCCATTCGC BaHl6104 CCACATCAAAAGAGCAGTCAG 17922 CCACATCAAA-GAGCAGTCAG 17923 bah22p07 GGTGGAATTCTTTGGTGTCCC 17924 GGTGGAATTCCTTGGTGTCCC bah22p07 CCAGATGCTTTCATCCTCTCA 17925 CCAGATGCTTCCATCCTCTCA

TABLE 22-20 Haruna Nijo SEQ Clones H602 ID NO: baak21j02 GCTCTTTGTCGGTGCCTTGCA 17926 GCTCTTTGTCCGTGCCTTGCA bags21o12 TGCCAAACGGAACGGTGCCGA 17936 TGCCAAACGGGACGGTGCCGA bag21o12 TGAGCGACCGCGTGTATATGT 17937 TGAGCGACCGTGTGTATATGT bags21o12 TCTTCTGCCCTGCCGGAACAT 17938 TCTTCTGCCCCGCCGGAACAT bags9b14 ATGTGCAGCAGATGAGATGAG 17939 ATGTGCAGCAAATGAGATGAG bags9b1  CTGCCCAGATATATGATTAGG 17940 CTGCCCAGATGTATGATTAGG bags9b14 GGAATGGAATATGCGAAATAG 17941 GGAATGGAATGTGCGAAATAG bags9b14 GATTTAAAAGGCTTCTTCCAG 17942 GATTTAAAAGACTTCTTCCAG bags9b14 AGATGAGAAACGACGCGACAC 17943 AGATGAGAAATGACGCGACAC bags9b14 ACTCACATACGCAGCTTTCAA 17944 ACTCACATACACAGCTTTCAA baak1a17 CAGGGGGCAGAGTCATAAGAT 17946 CAGGGGGCAGCGTCATAAGAT baak1a17 AGCACTATAATCGTCCGAAAC 17947 AGCACTATAACCGTCCGAAAC bags1p04 AAACTTACTTGGTGCACGACT 17951 AAACTTACTTAGTGCACGACT bags1p04 TAATAAATTTACAAACTCAAA 17952 TAATAAATTTCCAAACTCAAA BaAL17O03 TAAGGTAGGTAGGTTGGTTGG 17953 TAAGGTAGGTTGGTTGGTTGG baal8e17 CTACTGGCTCTGTCGCTGGAT 17954 CTACTGGCTCCGTCGCTGGAT baal839e17 TGGTGGTGGACAAACATATAT 17955 TGGTGGTGGATAAACATATAT BaH39L18 CAATGGGCGGCGCGGCGCAGC 17958 CAATGGGCGGGGCGGCGCAGC BaH39L18 CGCAGACAAGTCACTGGCAGC 17959 COCAGACAAGCCACTGGCAGC bags18e18 CCAGTTGCCCCCACGCTGCAG 17960 CCAGTTGCCCGCACGCTGCAG bags18e18 TTGAGCAGATGGTCAGTGTTT 17961 TTGAGCAGATTGTCAGTGTTT bags18e18 CTGTAGGCAATGTGTGGATGT 17962 CTGTAGGCAAAGTGTGGATGT

TABLE 23-1 Haruna Nijo SEQ Clones H602 ID NO: BaGS20L10 CGCGACAAACCGCGGAGCACA 17963 CGCGACAAACAGCGGAGCACA BaGS20L10 CTACCGATGACCAACCGAGTG 17964 CTACCGATGATCAACCGAGTG basd15p22 ACAGGTGCTCCATCGACTCAC 17965 ACAGGTGCTCGATCGACTCAC bags7b16 TCGTCACTGTTGCAAATCATA 17966 TCGTCACTGTCGCAAATCATA bags7b16 AGGACACTGTTACAGGTTAAG 17967 AGGACACTGTGACAGGTTAAG BaAK22H13 TTTCAGTTCTGTCGATGCAGT 17969 TCTCAGTTCTATCGATGCAGT BaAK22H13 TTATCTACCTTTCAGTTCTGT 17970 TTATCTACCTCTCAGTTCTAT BaH41L14 ATCAAGCTTACAAAACAGAAT 17973 ATCAAGCTTATAAAACAGAAT BaH41L14 TTGCAACCCCACCACCATCAA 17974 TTGCAACCCCGCCACCATCAA BaAK24H17 TGCATCAGAACGAATGTGCAT 17975 TGCATCAGAATGAATGTGCAT bast21A0602 ATATACCTTCTACCTTACATA 17976 ATATACCTTCCACCTTACATA BaAL27L20 ACCGCTGCAAGGGATCCTGCT 17977 ACCGCTGCAAAGGATCCTGCT BaAL27L20 CTCATTTCTGTGACGACCGCT 17978 CTCATTTCTGGGACGACCGCT BaAL27L20 ATGTCGGTCCAATAAATACTT 17979 ATGTCGGTCCGATAAATACTT BaAL27L20 GAGATTGCACAAAACTACATG 17980 GAGATTGCACGAAACTACATG bags34p10 TGCATCAGAACGAATGTGCAT 17981 TGCATCAGAATGAATGTGCAT baes34p10 ACCACCATGGGTTCTATGTCC 17982 ACCACCATGGATTCTATGTCC BaAL29B07 GGTTGTGTTGCCGCACGAACG 17986 GGTTGTGTTGTCGCACGAACG baak20o16 TAACAGCATTGTCGACAGTCA 17987 TAACAGCATTATCGACAGTCA baak20o16 GGATGTGCAGTGGCACCTACA 17988 GGATGTGCAGGGGCACCTACA BaH36B07 AGCAGCATGATGAGGTCCTTG 17991 AGCAGCATGACGAGGTCCTTG BaH36B07 AGCCGGAGGTTAGCAGGGCCG 17992 AGCCGGAGGTGAGCAGGGCCG BaH36B07 CGGCGTCCTCTTCCTCGGCAT 17993 CGGCGTCCTCCTCCTCGGCAT BaH36B07 GACCTCCTCCATCTTGCTCTC 17994 GACCTCCTCCGTCTTGCTCTC BaSD3C20 TCATACAGTAGCGGCGGCGGG 17997 TCATACAGTACCGGCGGCGGG

TABLE 23-2 Haruna Nijo SEQ Clones H602 ID NO: BaSD3C20 CCATGTATCAGATTGTCTCAT 17998 CCATGTATCAAATTGTCTCAT BaSD3C20 TAAAAATCCCTTGCGTTCACC 17999 TAAAAATCCCCTGCGTTCACC bastl17G0113 ATTATAGAAGGTTATCTTTCA 18000 ATTATAGAAGATTATCTTTCA baak11h14 CAATGGTGTGCAGACACAGGT 18001 CAATGGTGTGTAGACACAGGT baak11h14 CGGAGAAGAATGCTTCAAGGT 18002 CGGAGAAGAAGGCTTCAAGGT baal33a18 CATCCTTTGAGGTGCTGATAC 18004 CATCCTTTGAAGTCCTGATAC baal33a18 TCTCAAGAGGTTCAACTAACG 18005 TCTCAAGAGGCTCAACTAACG baal33a18 AGAATCAATCGTCAACTAGTA 18006 AGAATCAATCATCAACTAGTA baal33a18 AGAAGTGGACATTATCTTGCT 18007 AGAAGTGGACGTTATCTTGCT baal33a18 ACTTGACTCATCTATGTCCTA 18008 ACTTGACTCAACTATGTCCTA bah28a18 TGTTGAGTGCGTGTTCTTCCA 18009 TGTTGAGTGCATCTTCTTCCA bah28a18 GTTCACAGAAAAAAGTAGCTC 18010 GTTCACAGAAGAAAGTAGCTC bah28a18 CGCCGTCATACGCAGAGAGGA 18011 CGCCGTCATATGCAGAGAGGA bah28a18 CATCGTTTGCCTCATTGCTTC 18012 CATCGTTTGCTTCATTGCTTC bags39o04 CATTGTATCAGCATCTCGGAT 18013 CATTGTATCAACATCTCGGAT bags39o04 GGGGGTCCTCGACGACGTCCT 18014 GGGGGTCCTCAACGACGTCCT bags39o04 AATAAGGCTTCCGTAGACACC 18015 AATAAGGCTTTCGTAGACACC bags39o04 GTCAATTGACAGCGCCCCCTC 18016 GTCAATTGACGGCGCCCCCTC BaH48H04 CCCCTCCGATCAGCGGATCAT 18017 CCCCTCCGATTAGCGGATCAT bags4p16 CCGTTCCTTCGTGCGTATGCT 18018 CCTTTCCTTCCTGCGTATGCT bags4p16 TTGCTCCACCGTTCCTTCGTG 18019 TTGCTCCACCTTTCCTTCCTG basd24j22 TTGCCTGCTTTGTCAGGCGAG 18020 TTGCCTGCTTCGTCAGGCGAG basd16p15 ATACACCACAGGACGCCATTA 18021 ATACACCACATGACGCCATTA basd16p15 AGTACAGGTACAAGAAATGAT 18022 AGTACAGGTATAAGAAATGAT bags15k16 CTTTGTCCTCTAATGTCAGAT 18023 CTTTGTCCTCCAATGTCAGAT

TABLE 23-3 Haruna Nijo SEQ Clones H602 ID NO: bags15k16 GCCTTGCCATCGGTCTTCCTC 18024 GCCTTGCCATTGGTCTTCCTC bast23D1208 TGTGCTCGACGGTCTTCAGTT 18025 TGTGCTCGACAGTCTTCAGTT bast23D1208 AAACATAACCAATCGTGACGA 18026 AAACATAACCGATCGTGACGA baaG2b22 TTAGTCGTCCATATATCTTTT 18027 TTAGTCGTCCCTATATCTTTT baaB2b22 CATCATCTCTGTTGTATACTT 18028 CATCATCTCTATTGTATACTT baaB2b22 TGTATACTTGCCACACACCCG 18029 TGTATACTTGTCACACACCCG baaB2b22 ACGAAGTGCTGAGAGATAATA 18030 ACGAAGTGCTCAGAGATAATA baaB2b22 ACAAAATGACATGTTAATACA 18031 ACAAAATGACCTGTTAATACA baaB2b22 ACTTAGTTGATGGGTTAAAAG 18032 ACTTAGTTGACGGGTTAAAAG baaB2b22 CCACTGATATTATAGTACCTA 18033 CCACTGATATGATAGTACCTA baaB2b22 GTGACGGGTTACCCAAATATA 18034 GTGACGGGTTGCCCAAATATA baaB2b22 CACTCAATATGTATACTACTT 18035 CACTCAATATATATACTACTT BaH19L09 CACCCGACTCCATCAGGTTCT 18036 CACCCGACTCGATCAGGTTCT BaH19L09 ACACCGAGGCAGCGTTCTGGA 18037 ACACCGAGGCGGCGTTCTGGA BaH19L09 CGCATCTCGTTACCTTGGCGG 18038 CGCATCTCGTCACCTTGGCGG bags38a17 CAGATAAACTCTCAAGAATGA 18039 CAGATAAACTTTCAAGAATGA BaAK39I11 GCTTGCTGCCGTCGGTCGTGA 18040 GCTTGCTGCCATCGGTCGTGA BaSD3I24 CTCTAAGGAGGATAGAGCAAC 18041 CTCTAAGGAGAATAGAGCAAC BaSD3I24 TGACGACGACATCGTACATGT 18042 TGACGACGACGTCGTACATGT BaSD3I24 TCATCAGGAACACAAGGGTAT 18043 TCATCAGGAATACAAGGGTAT bah13l23 CCATATGGTAGACAGTATGCC 18046 CCATATGGTATACAGTATGCC bah13l23 ATAAAATTTCCGAGAACCCAA 18047 ATAAAATTTCTGAGAACCCAA bah13l23 TTTCATAAATACTCTATTCTT 18048 TTTCATAAATTCTCTATTCTT bah13l23 TTGTGCATCCATTTTTTATGT 18049 TTGTGCATCCGTTTTTTATGT bah13l23 TCGAGTTTGCGGATCTCCGTA 18050 TCGAGTTTGCAGATCTCCGTA

TABLE 23-4 Haruna Nijo SEQ Clones H602 ID NO: bah13l23 ACCCAAGTAATCCATATGGTA 18051 ACCCAAGTAACCCATATGGTA BaAL26H21 TGCATCGCTCATCAAACATGA 18052 TGCATCGCTCGTCAAACATGA BaAL26H21 CACACTGCACGGCAGAAAACT 18053 CACACTGCACCGCAGAAAACT BaAL26H21 CGCCGAAAAGCGTCGCGGCGG 18054 CGCCGAAAAGTGTCGCGGCGG BaAL26H21 CGAGATCTTCCGAAGGGTATA 18055 CGAGATCTTCTGAAGGGTATA BaAL26H21 AATCCAGACACCCTAAGACAC 18056 AATCCAGACATCCTAAGACAC baak32l16 GCAGGTTGTCAAAGCCGATGA 18060 GCAGGTTGTCGAAGCCGATGA baak32l16 ACTGCACCTGCCGCAGGTTGT 18061 ACTGCACCTGACGCAGGTTGT baak32l16 AGCCGATGATGCGGACGTAGG 18062 AGCCGATGATACGGACGTAGG baak32l16 GGTTACTTCAACTTGCACTTT 18063 GGTTACTTCATCTTGCACTTT baak32l16 TCAGGTAGTCGACCTGCTTCA 18064 TCAGGTAGTCAACCTGCTTCA baak32l16 TGTGTGGCGTGGGTGCACCCG 18065 TGTGTGGCGTCGGTGCACCCG baak32l16 AGTATCGGCCATCGTAGTACC 18066 AGTATCGGCCGTCGTAGTACC baak32l16 TGACCTCCTCGACCTCATTGA 18067 TGACCTCCTCGACCTCATTGA baak32l16 GGAGGGCCTCAGTGGTGAGGG 18068 GGAGGGCCTCCGTGGTGAGGG bags5c02 TCCCAGGCCACAACTCCTCTC 18073 TCCCAGGCCAAAACTCCTCTC bags35p07 GCTTCGGTGGGACTGGCTTCT 18074 GCTTCGGTGGCACGGGCTTCT bags35p07 TCGGTGGGACTGGCTTCTTGT 18075 TCGGTGGCACGGGCTTCTTGT BaAK24B09 CCTGTGGGAAGACCATGATGT 18076 CCTGTGGGAACACCATGATGT BaAK24B09 CGGCGGACGGCAGGTTGTTGG 18077 CGGCGGACGGGAGGTTGTTGG BaAK24B09 AGAGCTTGATTAGCCGCTGCC 18078 AGAGCTTGATCAGCCGCTGCC bags33a11 TTTCGTCATCATCAAAGCGAG 18079 TTTCGTCATCGTCAAAGCGAG bags33a11 TCCGAGTAAGTCCGTGCAAGT 18080 TCCGAGTAAGGCCGTGCAAGT bah56a24 ACTTGGGCGCTGCCTCCAGCT 18082 ACTTGGGCGCTGCCTCCAGCT bah56a24 GCTCCTTCTTTTCCACGCTCT 18083 GCTCCTTCTTCTCCACGCTCT

TABLE 23-5 Haruna Nijo SEQ Clones H602 ID NO: BaSD21D14 GACTCCATTCGGTTCCAGCAA 18084 GACTCCATTCCGTTCCAGCAA BaSD21D14 ACATTGCTTCTCCCACATGGT 18085 ACATTGCTTCCCCCACATGGT BaSD21D14 ACAAAGAAGGCCGTTCATGAT 18086 ACAAAGAAGGTCGTTCATGAT BaSD21D14 TGATGATCCAGCAAACAAGGG 18087 TGATGATCCAACAAACAAGGG basd1a17 TCTGTTGTACTTTATGACAAT 18088 TCTGTTGTACCTTATGACAAT basd1a17 TCATGTCAATGCAAATCTTTA 18089 TCATGTCAATACAAATCTTTA basd1al7 TGGTTCTATTTTAGTTTTATT 18090 TGGTTCTATTGTAGTTTTATT kr70g0113 CTTGTTATTCCGAATTGGAAA 18092 CTTGTTATTC-GAATTGGAAA 18093 basd15f08 ATAATATAAGTGTGTTTTTGT 18094 ATAATATAAGAGTGTTTTTGT basd15f08 GCTTCTTGACGTTGGTTCGGT 18095 GCTTCTTGACATTGGTTCGGT baal35h05 GGATCTTGTGATATAGCACTT 18101 GGATCTTGTGGTATAGCACTT baal35h05 AGCTTCCTCATCCCAAGCGTC 18102 AGCTTCCTCAGCCCAAGCGTC baal35h05 AAGATAGATACTTGCAGCAAC 18103 AAGATAGATAGTTGCAGCAAC baal35h05 ACCAGCTAGACCACCAGCTTC 18104 ACCAGCTAGATCACCAGCTTC baal35h05 CTGATTCAATTGACTCCTAAC 18105 CTGATTCAATCGACTCCTAAC baal35h05 ACGGGATTCAAAGAGGGATCT 18106 ACGGGATTCAGAGAGGGATCT baak14a24 CGTGGTACTTGTCGCCGTTGA 18108 CGTGGTACTTATCGCCGTTGA baak30d07 AAAAACATGTCGCATACCACA 18109 AAAAACATGTTGCATACCACA baak30d07 CGTGCACTCAGCAAGTTCCTC 18110 CGTGCACTCACCAAGTTCCTC kr14c0305 GAGACCGGGCCGGATCCGGCC 18112 GAGACCGGGCGGGATCCGGCC bast74c0705 CTAGCAGCAGCAGGAGGACTA 18115 CTAGCAGCAGGAGGAGGACTA baal4g17 TCGCAAGGCATCACACGCAAA 18116 TCGCAAGGCAACACACGCAAA baal4g17 CTAGCAGCAGCAGGAGGACTA 18117  CTAGCAGCAGGAGGAGGACTA bastl43H0515 AAGCCGATCATACACACACAC 18118 AAGCCGATCACACACACACAC BaH58M22 GCGTACGTGCACGAGCCATGC 18119 GCGTACGTGCGCGAGCCATGC

TABLE 23-6 Haruna Nijo SEQ Clones H602 ID NO: BaH58M22 CGTGGTACTTATCGCCGTTGA 18120 CGTGGTACTTGTCGCCGTTGA bags10i21 CGAACGCGTCACTGTAAGGAA 18121 CGAACGCGTCGCTGTAAGGAA bags10i21 ACAAAATGGCAACTTCAGGGG 18122 ACAAAATGGCGACTTCAGGGG bah20h16 CGTCGCAAACATTATTGCCAA 18123 CGTCGCAAACGTTATTGCCAA bah20h16 ATGGGTCAGGTATCGAAGCCG 18124 ATGGGTCAGGAATCGAAGCCG bah20h16 ATCCTACGGTTAACGGTGAAG 18125 ATCCTACGGTCAACGGTGAAG BaH17P13 GCTGAACATACCCCTTTTCAC 18126 GCTGAACATATCCCTTTTCAC baak16e24 ACTCGCATGCCTTCAACAGCA 18129 ACTCGCATGCTTTCAACAGCA baak16e24 CTGAGCTGACACACACAAGAT 18130 CTGAGCTGACCCACACAAGAT baak16e24 ATGCTCACAT-TTTCTACACA 18131 ATGCTCACATATTTCTACACA 18132 baak16e24 TATTCGAACC-CTGCTATGCT 18133 TATTCGAACCACTGCTATGCT 18134 BaSD18F09 CCGCGCTGGATGCGAAGTTCC 18135 CCGCGCTGGACGCGAAGTTCC BaSD18F09 CGAGGTTCGCCAGGTTGCCGT 18136 CGAGGTTCGCTAGGTTGCCGT BaSD18F09 GAAGGCGCTGGTGAAGGCCGC 18137 GAAGGCGCTGCTGAAGGCCGC BaSD18F09 GCGCGATGTTGCCCATCTTGA 18138 GCGCGATGTTTCCCATCTTGA baet42A0501 AGCTGATCCTCACCTGGCCCT 18139 AGCTGATCCTGACCTGGCCCT baet42A0501 GCCGTTGATCACCTTGTTAAT 18140 GCCGTTGATCGCCTTGTTAAT baet42A0501 AATTCACCTTGGAGCAGCTGA 18141 AATTCACCTTCGAGCAGCTGA baet42A0501 GGCCCTGCGAGCCGGTCAACG 18142 GGCCCTGCGAACCGGTCAACG baet42A0501 CACCATGGCCGAGGCGAAGGC 18143 CACCATGGCCAAGGCGAAGGC baak20l07 GCTTGCGAGCCCTGCCGACTA 18144 GCTTGCGAGCGCTGCCGACTA baak16e20 TTCAAAAGTGAGAAAATACCA 18145 TTCAAAAGTGGGAAAATACCA baak16e20 AATTCTGCTTGGGCTGTTCCG 18146 AATTCTGCTTCGGCTGTTCCG basd13m14 GGACATCAAAGCTACTTGTAT 18147 GGACATCAAATCTACTTGTAT basd13m14 GGTAACCTGAATTTATTATAG 18148 GGTAACCTGATTTTATTATAG

TABLE 23-7 Haruna Nijo SEQ Clones H602 ID NO: basd13m14 TACCTACAGATGAGGCAAGTT 18149 TACCTACAGACGAGGCAAGTT basd13m14 TTTCACAGCCCGTGAGGGACA 18150 TTTCACAGCCGGTGAGGGACA bags20g23 TAAATAAATATAATTCTGAAG 18151 TAAATAAATACAATTCTGAAG bags20g23 TTCTGAAGGTTGTTACTCCCT 18152 TTCTGAAGGTCGTTACTCCCT basd1l17 CGATGGCGAATGACTTCAAGA 18153 CGATGGCGAACGACTTCAAGA basd1l17 AACGGACCCTGCGGCGGCTCA 18154 AACGGACCCTTCGGCGGCTCA basd1l17 AGGAGTGGCCGATGATGTACG 18155 AGGAGTGGCCAATGATGTACG basd1l17 GAAGGAACGAATAGGCGTAGT 18156 GAAGGAACGAGTAGGCGTAGT basd1l17 GCGTCACCGACGGGCCCCTGC 18157 GCGTCACCGATGGGCCCCTGC basd26l01 TGCCATAGGTTTATCGTTCCG 18158 TGCCATAGGTCTATCGTTCCG basd26l01 TATCGTTCCGTTTACCTGTTT 18159 TATCGTTCCGATTACCTGTTT basd26l01 CGTTCCAAGGGCGCGCTCTTG 18160 CGTTCCAAGGTCGCGCTCTTG basd26l01 TCGCCTGTAGTGACGAGGACG 18161 TCGCCTGTAGCGACGAGGACG baal37j18 GGGTGGTCCAGATGACTAAGT 18162 GGGTGGTCCAAATGACTAAGT baal37j18 GGAGGGCAGATCTGATCTCTT 18163 GGAGGGCAGACCTGATCTCTT baal37j18 GACAGAAAGAGGACACACGGA 18164 GACAGAAAGAAGACACACGGA baal37j18 CAAGCCAGTTCATATACATAT 18165 CAAGCCAGTTGATATACATAT bags7i05 AAAAGGGATGATGGAATACAA 18166 AAAAGGGATGGTGGAATACAA bags7i05 CGGGTTCAGGCTTGGGCTCTG 18167 CGGGTTCAGGTTTGGGCTCTG bags7i05 ATCAATTGTCCGTCATCGGCG 18168 ATCAATTGTCAGTCATCGGCG bags7i05 CAAGGAATGCAAAAAAGATGC 18169 CAAGGAATGCTAAAAAGATGC bah60m17 AAATCTGTTCAGTTGTGTTGC 18170 AAATCTGTTCGGTTGTGTTGC bah60m17 GAGAATGTTATAACTAGGAGC 18171 GAGAATGTTAGAACTAGGAGC bah60m17 TTGAGGTGCACGGAAGGTGTG 18172 TTGAGCTGCAAGGAAGGTCTG bah60m17 TTGGATCTTCACCGCTGGCAT 18173 TTGGATCTTCGCCGCTGGCAT

TABLE 23-8 Haruna Nijo SEQ Clones H602 ID NO: bah60m17 GTTGCCGTCACAATCATCGAT 18174 GTTGCCGTCAGAATCATCGAT bah60m17 GAGGCACCTACTAAAATGGTA 18175 GAGGCACCTAGTAAAATGGTA bah60m17 AAAACCTGCACCAGTTTGGAT 18176 AAAACCTGCAGCAGTTTGGAT bags21l01 GCCTCCGCGGTAGGTTCTTCC 18177 GCCTCCGCGGGAGGTTCTTCC bags21l01 ACTCGTCAGCATGCTTCTTCT 18178 ACTCGTCAGCGTGCTTCTTCT baes21l01 TTCCTCTTGGTGCTTCCTCTT 18179 TTCCTCTTGGGGCTTCCTCTT baes21l01 TTGGACTTCTCTTTCTCCTGC 18180 TTGGACTTCTGTTTCTCCTGC bags21l01 GCTAGCATCAGCATCAGCTGC 18181 GCTAGCATCAACATCAGCTGC baet31e0109 CAATATTTCTGACCCGTCGTG 18182 CAATATTTCTAACCCGTCGTG baet31e0109 CATATGCGCGATCGTGCACAT 18183 CATATGCGCGGTCGTGCACAT bags34m12 GTACGTCTTCGTACCGGATTC 18184 GTACGTCTTCATACCGGATTC bags34m12 CCTAGTTTTTATAGTTAGCTA 18185 CCTAGTTTTTGTAGTTAGCTA bags34m12 TTTATTCTGGGATCCTTATTT 18186 TTTATTCTGGAATCCTTATTT bags34m12 TTCGCTGGATTCTAGGAGTCC 18187 TTCGCTGGATACTAGGACTCC bags34m12 ATACCACTCAGAATCTAGGTC 18188 ATACCACTCAAAATCTAGGTC bah50n02 CTGCTCCCACTGAGGCGCCGA 18189 CTGCTCCCACCGAGGCGCCGA BaH50M11 TGCTTCATCACCTATCACTAT 18190 TGCTTCATCATCTATCACTAT BaH50M11 AATTTTGGGGTGGAGGGAGTA 18191 AATTTTGGGGCGGAGGGAGTA bast27e1010 TACCTTTCGCAAGGCGGAGCG 18192 TACCTTTCGCGAGGCGGAGCG bast27e1010 TACTGGTCATGAGGCGGGAGC 18193 TACTGGTCATCAGGCGGGAGC bags23c12 TCTCGGTTCCAGTTTCAGCCT 18194 TCTCGGTTCCCGTTTCAGCCT bah51m11 ATTTATCCGTCGAGACCGAGA 18195 ATTTATCCGTGGAGACCGAGA baak20d17 GCGCAGGGTGCAGTTCTCCTG 18196 GCGCAGGGTGTAGTTCTCCTG bags23l21 GCCTCAATTAAAAGATTGGAA 18197 GCCTCAATTATAAGATTGGAA bags23l21 CAGGGCATCCCATGTGACAAC 18198 CAGGGCATCCGATGTGACAAC

TABLE 23-9 Haruna Nijo SEQ Clones H602 ID NO: bags23l21 CGTGCCATATCAGGTTCAATC 18199 CGTGCCATATGAGGTTCAATC bags23l21 ATGCTCGTCTCGCCTCGAGAA 18200 ATGCTCGTCTTGCCTCGAGAA bags23l21 CTAGCTATCTAAAGGATGTCT 18201 CTAGCTATCTGAAGCATGTCT bags23d01 TTACTCTGAANAGAAATGAGT 18204 TTACTCTGAAGAGAAATGAGT bags23d01 TTTGAACACCACGGAGAGCCT 18205 TTTGAACACCGCGGACAGCCT baal29m02 ATACTCGAGGAGAAGAAGGGA 18207 ATACTCGACGGGAAGAAGGGA baal29m02 TATTGTTGCCTATACTCACGC 18208 TATTGTTGCCTATACTCACGC baal29m02 GGGTAGGGCTCCACATATTGT 18209 GGGTAGGGGTTGAGATATTGT bah17e21 CATGCGTAGAAAACCTTGGTA 18210 GGAGCTGAGAGAAGGTTGGTA bah56j18 GAGGCAAAAAGGAGAAGGATA 18211 GAGGGAAAAAAGAGAAGGATA bah56j18 GGTTAATTTTGGTGGTGGGAC 18212 GGTTAATTTTCCTCCTGGGAC BaAL34O13 AATAAGGGCGGTCGGTTAATT 18215 AATAAGGGGGATGGGTTAATT BaAL34O13 TTAGTAAGTGAATGTGTGGTA 18216 TTAGTAAGTGGATGTGTGGTA bags4n05 GCACCAACGCGCTCCAGACTA 18217 GCACCAACGCCCTCCAGACTA bags4n05 GCCACCTGTGGGCAATTATGA 18218 GCCACCTGTGCGCAATTATGA BaSD15P20 GCAAGCCATCATCATCCTCCT 18219 GCAAGCCATCGTCATCCTCCT bah27g02 TTAAAATGGCGAGATTGCCCA 18221 TTAAAATGGCCAGATTGCCCA bah27e02 TCATAGTAGAGACTACTACTC 18222 TCATAGTAGAAACTACTACTC bah27g02 AATCTACACTGAACAACATGC 18223 AATCTACACTAAACAACATGC bah27g02 GCAAGCGGGCGGCGTTGCAAT 18224 GCAAGCGGGCAGCGTTGCAAT bah27g02 CCTTCTAGACTAGCACACAGA 18225 CCTTCTAGACAAGCACACAGA bah27g02 CCCAGGAAAACCTCTACGCAC 18226 CCCAGGAAAAACTCTACGCAC bah27g02 TCTCAAAATATAAACAACCCA 18227 TCTCAAAATAGAAACAACCCA basd27m10 TTGCTTCCTCATAAACCCAGT 18228 TTGCTTCCTCGTAAACCCAGT basd23f16 TTGCCGAGTCGGAGCAAAAGC 18229 TTGCCGAGTCCGAGCAAAAGC

TABLE 23-10 Haruna Nijo SEQ. Clones H602 ID NO: basd23f16 TAATTTGCCGCGTTAGCTTGT 18230 TAATTTGCCGTGTTAGCTTGT basd23f16 TGGCCTCCATATTGCCGCCAC 18231 TGGCCTCCATTTTGCCGCCAC basd23f16 AGCACCTTGGAGCTGCTGAAC 18232 AGCACCTTGGTGCTGCTGAAC basd23f16 CATTGCATGTTTAGCACAACC 18233 CATTGCATGTATAGCACAACC basd23f16 TCGTATTCAGGATTCATTGCA 18234 TCGTATTCAGAATTCATTGCA bah16i19 ATACATTATCTTGAGTGACTA 18235 ATACATTATCGTGAGTGACTA bah16i19 GATTGTAGCAGTTAGATACAT 18236 GATTGTAGCAATTAGATACAT bah16i19 ATGATGCACAATTGCATCTCT 18237 ATGATGCACATTTGCATCTCT bah16i19 GTCTTTCTCTGGATAGAATAA 18238 GTCTTTCTCTCGATAGAATAA BaH50I20 GGCAAGTAGCAACATATGTCG 18239 GGCAAGTAGCGACATATGTCG bah28b24 CTGGTTGTCATCGGTAGCTCC 18241 CTGGTTGTCACCGGTAGCTCC basd14h21 CAAGTCATTTTACTTACTGAC 18252 CAAGTCATTTAACTTACTGAC basd14h21 GGGACGACAGATGACATCTTA 18253 GGGACGACAGGTGACATCTTA basd14h21 AATTTCACACATGTCATGGGA 18254 AATTTCACACGTGTCATGGGA basd14h21 GGGACTCTATGTCTCCCATCA 18255 GGGACTCTATATCTCCCATCA basd14h21 CAGAGCATTCTGAAAGCATCT 18256 CAGAGCATTCGGAAAGCATCT basd14h21 CTAACAATCCAAAGCTAAAAG 18257 CTAACAATCCGAAGCTAAAAG basd14h21 AGGCAATTTAAACACCACACG 18258 AGGCAATTTATACACCACACG bags13g18 CTCTAACCAGAGTCAGGATAT 18262 CTCTAACCAGCGTCAGGATAT BaH15D23 ACATTGCTTAAACACTATAGA 18263 ACATTGCTTATACACTATAGA BaH15D23 TTTCAGAATGCTGGAGGAATA 18264 TTTCAGAATGTTGGAGGAATA BaGS33I07 GAGTGTGGTAACGGTCACCCA 18266 GAGTGTGGTAGCGGTCACCCA BaGS33I07 GAGACTGTGATGCATTCAGCC 18267 GAGACTGTGACGCATTCAGCC BaGS33I07 ACCGCTTGCAGATGCCAACTA 18268 ACCGCTTGCAAATGCCAACTA BaGS33I07 AGTACCAATACCTCATAAGCA 18269 AGTACCAATAGCTCATAAGCA

TABLE 23-11 Haruna Nijo SEQ Clones H602 ID NO: basd27b02 TTGGCTTCAGGGCGGCATTCG 18270 TTGGCTTCAGAGCGGCATTCG bags30n12 GTACACACCCTGGGCTCTCAC 18271 GTACACACCCGGGGCTCTCAC bags30n12 CTTTGTCACCAAATCCAAGTT 18272 CTTTGTCACCGAATCCAAGTT basd3h13 TAAAGGCATCATTTAGCTAAT 18273 TAAAGGCATCCTTTAGCTAAT BaGS37L19 AGTAAAATGAATGAATTTACG 18274 AGTAAAATGAGTGAATTTACG BaGS37L19 GGGAAGTGATTTCTAGGCGCC 18275 GGGAAGTGATGTCTAGGCGCC BaGS37L19 GCTAGTTCTTAAAGAAAATCA 18276 GCTAGTTCTTTAAGAAAATCA BaGS37L19 TCAAGCATTGCGATGCATGTT 18277 TCAAGCATTGTGATGCATGTT bags20i15 TTATTGTTTGCGCTGCTGCAT 18279 TTATTGTTTGTGCTGCTGCAT bags20i15 TCAACGTTACCAAACACAAGT 18280 TCAACGTTACAAAACACAAGT bags20i15 TACAGCCAATACAACTAGGAT 18281 TACAGCCAATGCAACTAGGAT bags20i15 GCTGCATTGTTCACCGACCGC 18282 GCTGCATTGTCCACCGACCGC bags20i15 GCATCACCAAAATTGAAGTAC 18283 GCATCACCAACATTGAAGTAC bah56k07 GCATACTTCACCGTTGCAGCG 18285 GCATACTTCATCGTTGCAGCG bah56k07 GTCGCAGTCTCGTTCATCATC 18286 GTCGCAGTCTTGTTCATCATC bah56k07 CCAAACTTCACGGCTTGCTGA 18287 CCAAACTTCAGGGCTTGCTGA bags37d02 CATTCAGGTTCTTTTTGTTCT 18288 CATTCAGGTTTTTTTTGTTCT bags37d02 TATTTTCCACGGTAAATGGGA 18289 TATTTTCCACAGTAAATGGGA bags37d02 GAAATGAGTCGAGCGATGGTA 18290 GAAATGAGTCCAGCGATGGTA bags37d02 ACAAAGATCAGCGGGGAGGAC 18291 ACAAAGATCATCGGGGAGGAC bags37d02 ATAAGCCACAGCAAAGACAGA 18292 ATAAGCCACATCAAAGACAGA bags22l23 ATGGTTTGGTGTTTAGGGCTG 18295 ATGGTTTGGTTTTTAGGGCTG kr33a0901 CGCTGCCCAAGGTCGAGCTAC 18296 CGCTGCCCAATGTCGAGCTAC kr33a0901 ATGGCTTCAACAAACGCCGCA 18297 ATGGCTTCAAAAAACGCCGCA bastl38C0606 AGAGAAACGCTTCCTTTGGCC 18298 AGAGAAACGCGTCCTTTGGCC

TABLE 23-12 Haruna Nijo SEQ Clones H6102 ID NO: bastl38C0606 AGATGTTGCATCTGTCGTACC 18299 AGATGTTGCACCTGTCGTACC baak11J13 GATTGGCTGACTCTTGCAAGG 18300 GATTGGCTGATTCTTGCAAGG baak11j13 TTCCCTTCCTGTCACCGGTTC 18301 TTCCCTTCCTATCACCGGTTC bah58e19 TATTTTTCAATAACCAAGGAC 18302 TATTTTTCAACAACCAAGGAC BaGS31G22 AGAAAGAGACATGACAAATCA 18303 AGAAAGAGACGTGACAAATCA BaGS31G22 ACGCTGATGGTGAATCCGGAC 18304 ACGCTGATGGCGAATCCGGAC bah37h01 CAGATAGCATGTGTAGCTTCT 18305 CAGATAGCATTTGTAGCTTCT bah37h01 CCACAGGTGGGTCACTATCCA 18306 CCACAGGTGGTTCACTATCCA bah37h01 TCAGGCCAAAGACGATCATCT 18307 TCAGGCCAAAAACGATCATCT bah37h01 TAGCCTAATAGAATTTTTAGT 18308 TAGCCTAATATAATTTTTAGT baal29p13 AGTTTTTTAGGTTTCACTTAG 18309 AGTTTTTTAGATTTCACTTAG bags39a22 CTCTTTTTCCCAGCCCCATAA 18310 CTCTTTTTCCAAGCCCCATAA bahl9g10 GTATGCAGAGTGCTCCTGCCC 18311 GTATGCAGAGCGCTCCTGCCC baal31a14 TCGCTATGGCTGCAAAGAAGA 18312 TCGCTATGGCGGCAAAGAAGA bah50g15 AAGCTGAGGCAGCTCGCTCTT 18313 AAGCTGAGGCTGCTCGCTCTT bah50g15 TCCTTGTCCCGGTATTTTCTT 18314 TCCTTGTCCCAGTATTTTCTT bah51m12 TTGAATGAAGGTTTAAAAACT 18315 TTGAATGAAGATTTAAAAACT bags18k22 ACGATCTAACGCTGTACGAAA 18316 ACGATCTAACACTGTACGAAA bagsl8k22 CCTTCAGCGGTGCATCAGGTA 18317 CCTTCAGCGGAGCATCAGGTA bags20n21 CCTTTCCATCAGAGAATATGT 18318 CCTTTCCATCGGAGAATATGT basd17o21 CCCGATCTGCATAACCAAGAA 18319 CCCGATCTGCGTAACCAAGAA basd17o21 TCAAAAGTGACGGAAAGAAAA 18320 TCAAAAGTGATGGAAAGAAAA bah37g17 AAAATTGGACAAGGTTCTCTA 18321 AAAATTGGACGAGGTTCTCTA bags30l22 AGTAAGAAACATCAGCTAATA 18322 AGTAAGAAACGTCAGCTAATA bags30l22 GCTTCTTCTATGGTTGCATAT 18323 GCTTCTTCTACGGTTGCATAT

TABLE 23-13 Haruna Nijo SEQ Clones H602 ID NO: bags30l22 ATCTAGGATTCATCCCTTCCT 18324 ATCTAGGATTTATCCCTTCCT bast72G0113 CTTCCCATACGGACAGCACAA 18327 CTTCCCGTACAGACAGCACAA bast72G0113 TTAGCTTCCCATACGGACAGC 18328 TTAGCTTCCCGTACAGACAGC BaH30B05 CATGCTTCCGCTCAGATAATG 18329 CATGCTTCCGTTCAGATAATG BaH60B14 ACCCCTCCCTACCCAACCTGC 18330 ACCCCTCCCTCCCCAACCTGC BaH60B14 TTGCAATATGGGACCTTACAT 18331 TTGCAATATGTGACCTTACAT BaH60B14 CTGCACCTGATCTGCATGTCT 18332 CTGCACCTGACCTGCATGTCT BaH17B16 CAAAAAGATACGAGTGTTTGT 18333 CAAAAAGATATGAGTGTTTGT basd12n23 AGTTCGGCAGCTTTGTTGCCA 18334 AGTTCGGCAGTTTTGTTGCCA basd12n23 AAACGATGGAGATTAATCGTG 18335 AAACGATGGAAATTAATCGTG basd12n23 GATGTAGCAGATGATCTTACA 18336 GATGTAGCAGTTGATCTTACA basd12n23 GATGACATTTCACATGTCATT 18337 GATGACATTTTACATGTCATT basd12n23 CCTTGAGTTTCTTGCAAATAA 18338 CCTTGAGTTTATTGCAAATAA basd12n23 GATTCACTGGTCCATTCGGAT 18339 GATTCACTGGCCCATTCGGAT basd12n23 CAACAGCTTGTTCAGTGGTGC 18340 CAACAGCTTGATCAGTGGTGC basd12n23 TTGTGCGGACTTCAACAGCTT 18341 TTGTGCGGACATCAACAGCTT bah61a21 GCTATTTTATATCAATGATGA 18342 GCTATTTTATCTCAATGATGA baak19h17 TTCTAATCAGTGGTATAATAG 18343 TTCTAATCAGGGGTATAATAG baak19h17 GTCGTGCAGAGCGCCGTTTGT 18344 GTCGTGCAGAGCGCCGTTTGT bah47H2 AAGAGTTAGGAAAGAAGAGGC 18345 AAGAGTTAGGCAAGAAGAGGC bah47l12 AGCAGCGAGAAGACAGCTGGA 18346 AGCAGCGAGAAGACAGCTGGA baak18p11 AGCTGGCGTTTGATCTGTTGA 18347 AGCTGGCGTTGGATCTGTTGA baak18p11 ACAACAGAGAGATTATCACAA 18348 ACAACAGAGAAATTATCACAA baak18p11 AAAAGTTCTTCCTTTTAATCC 18349 AAAAGTTCTTTCTTTTAATCC baak18p11 TGGATCTGCTAGTGCTGGCGC 18350 TGGATCTGCTGGTGCTGGCGC

TABLE 23-14 Haruna Nijo SEQ Clones H602 ID NO: baet29h0715 CCTCCTTGGCCGCCGCGGATA 18351 CCTCCTTGGCTGCCGCGG-TA 18352 BaH13D11 GAAATCCTTCAGCATAGTTTG 18353 GAAATCCTTCGGCATAGTTTG BaH13D11 GTCTCAGACAAGGTACTTCCA 18354 GTCTCAGACACGGTACTTCCA BaH13D11 ACAGTACACACCGACTGAATA 18355 ACAGTACACAACGACTGAATA BaH13D11 ATAGAAGCACAATTGTTGAGA 18356 ATAGAAGCACGATTGTTGAGA baet39D1107 GACCGCCAACATTAGCGCGCC 18357 GACCGCCAACGTTAGCGCGCC BaH53E15 TATTAAGCTATGAAAAATATT 18358 TATTAAGCTATGAAAAATATT bags32o15 TTACTGCGACGCACGCACAGA 18359 TTACTGCGACCCACGCACAGA bags32o15 TAGAGTCGCGGCATCTTTTGT 18360 TAGAGTCGCGCCATCTTTTGT bags32o15 GTCTTACACCAGATACTTGGG 18361 GTCTTACACCCGATACTTGGG bast48a0701 AAGCTAGCAGCTCTCAAGGAC 18362 AAGCTAGCAGGTCTCAAGGAC bast62d0907 GTACTAGGGACACCTCCCACA 18363 GTACTAGGGAGACCTCCCACA bast62d0907 ACAGCGCACAGCGAGACGACG 18364 ACAGCGCACACCGAGACGACG bah45p22 TCTGTGCTCACATGGAATGGG 18365 TCTGTGCTCATATGGAATGGG bah45p22 AATATGAAAATGAGAAGTAAC 18366 AATATGAAAACGAGAAGTAAC bags32H6 CAAAAAACTACCACGAGCAGA 18367 CAAAAAACTAACACGAGCAGA baak30j05 AACTAAAATACACTTTAACGG 18368 AACTAAAATATACTTTAACGG baak30j05 GTCTCTGACGAGCATTCATGA 18369 GTCTCTGACGGGCATTCATGA bah52a14 TTTCTCATCGATATCTCCCGA 18370 TTTCTCATCGGTATCTCCCGA bah52a14 TGTAACTTGGAACCACTCTTG 18371 TGTAACTTGGCACCACTCTTG bah58i22 AGGTGTGTATGAAATACCACT 18372 AGGTGTGTATCAAATACCACT BaH56L16 GTTTAGACTGAACAGCAAAGC 18373 GTTTAGACTGGACAGCAAAGC BaH56L16 TTTCTTTTTCCAATCCAGAAA 18374 TTTCTTTTTCAAATCCAGAAA bah52a21 CTGAAATTATTTTACTAACAT 18375 CTGAAATTATATTACTAACAT bah52a21 CCATAGATTTAGGTGACTGGA 18376 CCATAGATTTGGGTGACTGGA

TABLE 23-15 Haruna Nijo SEQ Clones H602 ID NO: bah52a21 ATAATATTAACACCTTGATAT 18377 ATAATATTAAAACCTTGATAT basd14g11 AACAACAGTTCCTGTTGACAG 18378 AACAACAGTTGCTGTTGACAG bags6a03 ACAAGAGGCAGATAAATTGAG 18379 ACAAGAGGCACATAAATTGAG bast72e0109 TTTTTTTTTTATTTTTTTTTT 18380 TTTTTTTTTTTTTTTTTTTTT bags21a21 CCTGGATCATTGCATAGATGT 18381 CCTGGATCATAGCATAGATGT bags21a21 AGTTGGGGTGCAACTCTGATA 18382 AGTTGGGGTGTAACTCTGATA bags21a21 CTACTTGGGTGTAAAGGTGTT 18383 CTACTTGGGTATAAAGGTGTT bah58p13 CTACATAGAAGCATGCAGGTG 18384 CTACATAGAAACATGCAGGTG bah58p13 TCGGAGGGAGAATTATAATTT 18385 TCGGAGGGAGTATTATAATTT bah58p13 TGTATGCCAACCGTACCTTCT 18386 TGTATGCCAATCGTACCTTCT bah27n22 GTACAAAATATCATACATATA 18387 GTACAAAATACCATACATATA bags20c13 GGTTATTGCACATAAAAGTGA 18388 GGTTATTGCAAATAAAAGTGA bags20c13 AACAAATGCAGGAGTTGCAGA 18389 AACAAATGCATGAGTTGCAGA bags20c13 GATATGTTCCCATGCATTTTT 18390 GATATGTTCCGATGCATTTTT bags20c13 TTTATCTCGTTATATGACGCT 18391 TTTATCTCGTCATATGACGCT bags30i14 TCGCACGCCGACTCAATTTTA 18392 TCGCACGCCGTCTCAATTTTA bass30i14 GATACGGAATCTACCTCTCAG 18393 GATACGGAATTTACCTCTCAG bags30i14 GCTCGTCCGGATGGATACGGA 18394 GCTCGTCCGGTTGGATACGGA BaAK17E11 GCGTAAGTAGGTCATCAAAAC 18395 GCGTAAGTAGATCATCAAAAC BaAK17E11 GAAATCCTTCAGCATAGTTTG 18396 GAAATCCTTCGGCATAGTTTG baal10l01 CACCACTAAG-GACAGACCAC 18399 CACCACTAAGAGACAGACCAC 18400 baal10l01 TGCGAAATCTCTGGTTCCTGA 18401 TGCGAAATCTTTGGTTCCTGA baak26e17 AAAGCACAATGGACTATCTCG 18402 AAAGCACAATAGACTATCTCG baak26e17 TATCTCCAGATGAGGTGATAC 18403 TATCTCCAGACGAGGTGATAC bags32d21 CTTCCTTTTCACCTTATAACG 18407 CTTCCTTTTCGCCTTATAACG

TABLE 23-16 Haruna Nijo SEQ Clones H602 ID NO: bags32d21 CTCTTTCCAGCAGTTTGGTCC 18408 CTCTTTCCAGAAGTTTGGTCC bags32d21 GTTAAGCAGGTGCTGGATCCT 18409 GTTAAGCAGGGGCTGGATCCT bah54j22 TAACTGACTTCTACAAGAAAA 18410 TAACTGACTTTTACAAGAAAA bah54j22 AGATTATTGAACGGACCACAT 18411 AGATTATTGATCGGACCACAT bah54j22 GCCAAAACTAAGAGAGGACGA 18412 GCCAAAACTATGAGAGGACGA bah54j22 GTTGGTGTCATAGAAGATTAT 18413 GTTGGTGTCACAGAAGATTAT bah54j22 TTTTGGAGCAATGACACGAGC 18414 TTTTGGAGCACTGACACGAGC bah13f11 GCTTATTGGTAAACTGCATCA 18417 GCTTATTGGTGAACTGCATCA baal4f01 AAACGCACTCGTGGCCCACAC 18421 AAACGCACTC-TGGCCCACAC 18422 baal4f01 AAACTCTAAGTTGGGAGACCA 18423 AAACTCTAAGATGGGAGACCA baal4f01 CTCGATTGGCATGGGATCAAC 18424 CTCGATTGGCNTGGGATCAAC baal4l21 TCTTCGCTTTTTGATTGATGG 18426 TCTTCGCTTTCTGATTGATGG BaAK31O14 CAGCTCATCCGCATCGCTGGA 18428 CAGCTCATCCACATCGCTGGA baafl3d17 TCCACACGATTTCAGCAACTT 18430 TCCACACGATCTCAGCAACTT baal13d17 TGAGCCCCTGGGCGAGCGTGC 18431 TGAGCCCCTGCGCGAGCGTGC baal13d17 AGGCTTCAGAATGGCCGTTTG 18432 AGGCTTCAGAGTGGCCGTTTG baal13d17 GCTTATTTCCCCACGGGTTCT 18433 GCTTATTTCGAGACCCGTTCT bags22j12 CCCACGCACAATTCGGAAGCA 18434 CCCACGCACAGTTCGGAAGCA bags33p05 AAGACGCCATGTCAGAATAAG 18435 AAGACGCCATATCAGAATAAG bags33p05 CAAAATGACCGAACACATGAA 18436 CAAAATGACCAAACACATGAA bags33p05 TTCGATCACTTCTCAGATCAT 18437 TTCGATCACTCCTCAGATCAT bags33j07 TCGGGTTCAGACATGCAATGA 18438 TCGGGTTCAGGCATGCAATGA bagsl5d19 TGCCACGGCTGCCGTAAGGGT 18441 TGCCACGGCTACGGTAAGGGT bah57o03 AAAAGAACTTTATGTTGTCGC 18447 AAAAGAACTTCATGTTCTCGC bah57o03 GGATTGGGCAAAGATGATATT 18448 GGATTGGGCACAGATGATATT

TABLE 23-17 Haruna Nijo SEQ Clones H602 ID NO: BaAK12F04 CTTGAAAACTCTGGCAGTATC 18454 CTTGAAAACTATGGCAGTATC BaAK12F04 TATGGTACAACAGAAACAATT 18455 TATGGTACAATAGAAACAATT BaAK12F04 TAAACCGACCCTTGATGTAGC 18456 TAAACCGACCATTGATGTAGC bah53j16 GCTCGAGTCTCAATCGTCTCC 18457 GCTCGAGTCTAAATCGTCTCC bah53j16 TTCATTCATCTGTTCTAACAC 18458 TTCATTCATCGGTTCTAACAC bah53j16 CTCCTAGGGACTCAAGACCCT 18459 CTCCTAGGGATTCAAGACCCT BaSD2E24 TCTCTCTTTTTCAGCATGAAG 18460 TCTCTCTTTTCCAGCATGAAG BaSD2E24 CGTAATCTTAAGATTCATATA 18461 CGTAATCTTAGGATTCATATA bags39h08 AGGATCTAGCGATACACCGTT 18462 AGGATCTAGCAATACACCGTT basd1n02 TGGCGGACGCGCCGTCGCGCA 18463 TGGCGGACGCACCGTCGCGCA bags20m21 CGCACACGGGTTGCAGTTCAC 18464 CGCACACGGGGTGCAGTTCAC baat10h19 GGATACCAAGAGTCCTGGAGA 18465 GGATACCAAGGGTCCTGGAGA basd12m11 CTTGCATACCTCTTACCGATT 18466 CTTGCATACCGCTTACCGATT basd12m11 ATTAGCATTTTTCTTAACATC 18467 ATTAGCATTTCTGTTAACATG bah29d24 CCTCCCTGCTTCTTTGCTGGC 18468 CCTCCCTGCTGCTTTGCTGGC bah29d24 TTACATACCTACCCCCCTCCT 18469 TTACATACCTTCCCCCCTCCT BaGS39P09 CAGATATTCTAAGCTAGATTA 18470 CAGATATTCTGAGCTAGATTA BaGS39P09 ATCCCTGTCGACATATCTTGG 18471 ATCCCTGTCGCCATATCTTGG BaGS31F17 CATGGGTGCCAATGAGCCCAA 18472 CATGGGTGCCGATGAGCCCAA BaGS23D08 ATTGAGTGATTGGTCTATGAC 18473 ATTGAGTGATGGGTCTATGAC BaGS23D08 CGTCACCAAATTTGTCAGAAA 18474 CGTCACCAAACTTGTCAGAAA bags39d15 ATGGTTGGAGCGCGACGCTGG 18475 ATGGTTGGAGTGCGACGCTGG BaGS34I17 ACCACAAAAATTCTGGCTTTC 18476 ACCACAAAAAATCTGGCTTTC BaGS34I17 ATGCATGATCCGTGTTGCCTG 18477 ATCCATGATCTGTGTTGCCTG BaH32N02 GCTGCCTGGAGGCAATTTTCC 18478 GCTGCCTGGAAGCAATTTTCC

TABLE 23-18 Haruna Nijo SEQ Clones H602 ID NO: BaSD18H19 CACGATCTATACATGTGTTCC 18479 CACGATCTATGCATGTGTTCC bast63A0101 GGCCCGAGTGGTTGCGATGCA 18480 GGCCCGAGTGATTGCGATGCA BaH50N04 GAGCGGCGCGCGGTACGGTGG 18481 GAGCGGCGCGTGGTACGGTGG BaH50N04 GTACGTAGCTTGCTTGGTCGC 18482 GTACGTAGCTCGCTTGGTCGC baal4a13 TATCGTATTGAGTGCTTTTCA 18483 TATCGTATTGTGTGCTTTTCA baal4a13 TTTATCAGCTGTGCATAAGTT 18484 TTTATCAGCTTTGCATAAGTT baal4a13 GAATGGATGACAACCACGATG 18485 GAATGGATGAGAACCACGATG baal4a13 TACCAGAACAGATGTCATTTT 18486 TACCAGAACAAATGTCATTTT baal41l18 GCCATCTAATAGTATTAGCAT 18487 GCCATCTAATGGTATTAGCAT baal41l18 TCCTCTTTCTTCCTTCTCCTT 18488 TCCTCTTTCTCCCTTCTCCTT baal41l18 ATATCTATTCGCAGGTAAGAC 18489 ATATCTATTCACAGGTAAGAC baal41l18 TTAAACAGGAAAGATAGAGAG 18490 TTAAACAGGAGAGATAGAGAG baal41l18 GTGTTACTGGTTACTTATCAT 18491 GTGTTACTGGGTACTTATCAT baal41l18 GTACTAHCAGTTAGCCGCCAT 18492 GTACTACCAGGTAGCCGCCAT baal41l18 ATAGAGAGAGTTTTATTTATA 18493 ATAGAGAGAGGTTTATTTATA baal41l18 TTGACAACTAGTTATATCTAT 18494 TTGACAACTAATTATATCTAT baal27e20 AAATGCATGTAAAGCACCAGC 18497 AAATGnATGTGAAGCACCAGC baal27e20 TCAGCTGGCAAGCTTGAAGGC 18498 TCAGCTGGCATGCTTGAAGGC baal27e20 GTTGCGTTTTCTCACCATCTT 18499 GTTGCGTTTTTTCACCATCTT baal27e20 GCCTACTTACCGTGATGAAAA 18500 GCCTACTTACTGTGATGAAAA bags37l16 CAGCACCAGGAGATGATGCAT 18501 CAGCACCAGGGGATGATGCAT baak34o06 TCGGAAGAGTAAATGTATCTT 18502 TCGGAAGAGTGAATGTATCTT baak34o06 TGATCACAGCAGTGAGGATTC 18503 TGATCACAGCCGTGAGGATTC baak34o06 CATCTTTGGAGCATGCTACAG 18504 CATCTTTGGAACATGCTACAG baak34o06 CGACAACTAACTCCGGACGGA 18505 CGACAACTAATTCCGGACGGA

TABLE 23-19 Haruna Nijo SEQ Clones H602 ID NO: baak34o06 ATCGGCGATACGATATTACAT 18506 ATCGGCGATATGATATTAGAT baal30i11 TATTGGTCAACTTTATACTAC 18507 TATTGGTCAAATTTATACTAC BaH62C15 GAATTAACTCCTGCCCCTCCT 18508 GAATTAACTCTTGCCCCTCCT baak16l19 TGCGCCACCGTCTCCGGGTTG 18512 TGCGCCACCGCCTCCGGGTTG kr41h0315 GTCGCTGTTCCGTCTGTGGAA 18514 GTCGCTGTTCAGTCTGTGGAA bah62i23 TTCCACAGACGGAACAGCGAC 18515 TTCCACAGACTGAACAGCGAC bah52i24 CGTCTGGTGATTCTGCCTTTG 18516 CGTCTGGTGACTCTGCCTTTG bastl33a0301 CGTCTGGTGACTCTGCCTTTG 18517 CGTCTGGTGATTCTGCCTTTG bastl56A0301 TGCCACTACAAGTATATGCAG 18519 TGCCACTACAGGTATATGCAG baal39a03 ACTCAAGCTTCCATTTTTTCT 18520 ACTCAAGCTTGCATTTTTTCT baal39a03 CTTTCTCTGACGAAGAAACAA 18521 CTTTCTCTGAAGAAGAAACAA baal39a03 GTGGTATATTTTCCAAACTCA 18522 GTGGTATATTCTCCAAACTCA baal39a03 TCTCACAATCCCTGATATGTT 18523 TCTCACAATCGCTGATATGTT baal39a03 ATGCACATTTTCCGTGCGCGC  18524 ATGCACATTTACCGTGCGCGC baal39a03 ATTGTCGGGGAAAAATGTATG 18525 ATTGTCGGGGGAAAATGTATG baak34l03 TTCTTAGACATGCATTATGTG 18527 TTCTTAGACACGCATTATGTG baak34l03 TGCACATGAAGGAAATAGATG 18528 TGCACATGAAAGAAATAGATG baak34l03 ATGTCTGTTGATGTACTTGTG 18529 ATGTCTGTTGGTGTACTTGTG baak34l03 CATGGAAGTTCTCATAGGGAT 18530 CATGGAAGTTTTCATAGGGAT baak34l03 TACATCTATACAATGTCATGG 18531 TACATCTATATAATGTCATGG baak34l03 GTGATAAATTTTCTGTGTCAT 18532 GTGATAAATTCTCTGTGTCAT baak34l03 TAAACAAAGAAAAGCTTGATG 18533 TAAACAAAGATAAGCTTGATG BaSD20J15 ACTATGTATGCACAGACGCGC 18534 ACTATGTATGTACAGACGCGC bastl30f0711 CACAAACAAACGGGACCCCCA 18535 CACAAACAAAGGGGACCCCCA bastl30f0711 GTTTGCTTATATACTATACAC 18536 GTTTGCTTATTTACTATACAC

TABLE 23-20 Haruna Nijo SEQ Clones H602 ID NO: bast56G0713 ACCAGAAGCTCTCTACTACCA 18539 ACCAGAAGCTTTCTACTACCA bast56G0713 CAGCCGAACCCCTGGCTGGCT 18540 CAGCCGAACCGCTGGCTGGCT bastl02H0715 GATTTGGCATCAATGTTACAA 18543 GATTTGGCATTAATGTTACAA bastl02H0715 GTGTCAAAGGAAACTCCAAAA 18544 GTGTCAAAGGGAACTCCAAAA bags8i05 CACCGCACCGACTTTCTCGTT 18545 CACCGCACCGGCTTTCTCGTT basd18m04 GGCGACGCCGGAACCAACGAA 18547 GGCGACGCCGAAACCAACGAA bah28g01 CGTAGTCTTCCGGTGTTAGTG 18550 CGTAGTCTTCAGGTGTTAGTG basd1m13 ACGGCAAACCGAGACGAGAAC 18551 ACGGCAAACCCAGACGAGAAC bags37h19 AAGAAGGATATCTATGAGACG 18552 AAGAAGGATAACTATGAGACG bags37h19 TGTGTGTGTGATGATCGGTTT 18553 TGTGTGTGTGGTGATCGGTTT bags37h19 CTACGTCACCAACGACAGAAA 18554 CTACGTCACCGACGACAGAAA bah21a10 GATGCCTCCTGGCCCTCGGCG 18556 GATGCCTCCTCGCCCTCGGCG bah21a10 CTCAGTCTCAGACCACACCTA 18557 CTCAGTCTCAAACCACACCTA bags23d06 AAAATCAATACTAAATACTCC 18558 AAAATCAATATTAAATACTCC BaGS19G10 TTTGCTTCACACCGGGGTTCT 18560 TTTGCTTCACGCCGGGGTTCT bah11b24 TCAAAAAGCAGAAGTGGGCTC 18561 TCAAAAAGCAAAAGTGGGCTC bah11b24 TAAGAAAGCTATGTATCAGCA 18562 TAAGAAACCTCTGTATCAGCA bah11b24 TACAAACAGTGAATAAGCCTG 18563 TACAAACAGTAAATAAGCCTG bah11b24 CAGGAAGTTCGTGCAGATTGC 18564 CAGGAAGTTGTTGGAGATTGC bah11b24 ATAATAATAGTTCAAACAGGG 18565 ATAATAATAGCTCAAACAGGG bah11b24 GTGGGCTCCGATGTATGTAAC 18566 GTGGGCTCCGGTGTATGTAAC bah11b24 TTAACACTTGATTACTGGTGA 18567 TTAACACTTGGTTACTGGTGA bah11b24 ATAAGCCTGTAATCTAGATAT 18568 ATAAGCCTGTGATCTAGATAT bast39d0408 TGAGTTTGTATTGCTGCAAAT 18569 TGAGTTTGTACTGCTGCAAAT bast39d0408 TGCTACAATACAAATGGAATG 18570 TGCTACAATATAAATGGAATG

TABLE 23-21 Haruna Nijo SEQ Clones H602 ID NO: bast39d0408 GAATGATCGCATAGCGAGAAC 18571 GAATGATCGCGTAGCGAGAAC bast39d0408 CAATAATCGCGAGTTGGTGCA 18572 CAATAATCGCAAGTTGGTGCA baak33e08 TGAGAAACACAGCAAACGATG 18573 TGAGAAACACGGCAAACGATG BaAL33B08 ACTCAAATTGAACGGTCAGTA 18575 ACTCAAATTGCACGGTCAGTA BaAL33B08 CGTTCACTGTATACGGGCCAA 18576 CGTTCACTGTGTACGGGCCAA BaAL33B08 CCTCGGTCTCTCCTTGTCTCT 18577 CCTCGGTCTCCCCTTGTCTCT BaAL33B08 CGGTCAGTAACTGTGTCTCCG 18578 GGGTCAGTAATTGTGTCTCGG bags39e12 TGAGTACACTTATTAACCTGT 18579 TGAGTACACTCATTAACCTGT bags39e12 ACAGAGCTTCAGATAATTGGT 18580 ACAGAGCTTCTGATAATTGGT bags39e12 TGTCAATCAATGACTTGTACT 18581 TGTCAATCAAGGACTTGTACT bags39e12 TTCAGCAAGTCATTGAAGTGT 18582 TTCAGCAAGTAATTGAAGTGT bags39e12 ATCACGTGCCCGCAACCGCCA 18583 ATCACGTGCCTGCAACCGCCA bags33n09 ACCTTCCAATAAAATTTAGTT 18586 ACCTTCCAATGAAATTTAGTT baal32h09 ACTTGGCCAGTGGTCACAGCA 18587 ACTTGGCCAGNGGTCACAGCA baal32h09 CACCACCTGATCATCAGATTG 18588 CACCACCTGACCATCAGATTG baal32h09 CCGAGTCCGGTTGATTCTGGC 18589 CCGAGTCCGGNTGATTCTGGC baal32h09 GATTCTGGCGGGCTCCTGCTC 18590 GATTCTGGCGAGCTCCTGCTC BaAK20O17 ACTGCCCTCCGTCCTTTTACG 18591 ACTGCCCTCCCTCCTTTTACG BaAK20O17 GGAGTAGTTTGCAACACTGCC 18592 GGAGTAGTTTACAACACTGCC BaAK20O17 ATTTCTAAAATGTCTTATATT 18593 ATTTCTAAAAGGTCTTATATT bags32g10 CCGCATTCTCCCCTTCAAACA 18594 CCGCATTCTCTCCTTCAAACA baet33E1210 GAGCTCGTGCCGCTGCCGTCA 18595 GAGCTCGTGCGGCTGCCGTCA baet33E1210 GCCCGTGTGGGCACTGGGCAC 18596 GCCCGTGTGGACACTGGGCAC bah50o08 TTGATTCCTGCAGCCTTTTCC 18597 TTGATTCCTGTACCCTTTTCC bah50o08 CCGGCATCTCCGGTACTGCTT 18598 CCGGCATCTCTGGTACTGTTT

TABLE 23-22 Haruna Nijo SEQ Clones H602 ID NO: bah50o08 GATTCCTGCAGCCTTTTCCTG 18599 GATTCCTGTACCCTTTTCCTG bast46F0711 ATAACCCTACGACTCAGAAGG 18600 ATAACCCTACAACTCAGAAGG bast46F0711 GCCGACCCCGATACTAGCACG 18601 GCCGACCCCGTTACTAGCACG bast46F0711 GAGAGCAGGAAATATCTATCA 18602 GAGAGCAGGACATATCTATCA bast46F0711 ATCTATCATTTCAGGCCAAAT 18603 ATCTATCATTCCAGGCCAAAT bah16f05 TATCTCCTGGCACAGCGTTTT 18604 TATCTCCTGGGACAGCGTTTT bah16f05 GGAGCGTCAGGAACTGATTCA 18605 GGAGCGTCACAAACTGATTCA bah13o11 GCACCGTAGCGGAGGACATCC 18607 GCACCGTAGCAGAGGACATCC basd27i13 AAACTGTTACTCTACCGAACA 18609 AAACTGTTACGCTACCGAACA basd27i13 CTAGGATCTCGGTCAGCAGCT 18610 CTAGGATCTCAGTCAGCAGCT bags26e20 AAATGCTTCAAAAAGATATCA 18613 AAATGCTTCACAAAGATATCA bags26e20 GCGTGCATGTCACCAGTATAG 18614 GCGTGCATGTTACCAGTATAG bags26e20 CATCAAGAACGGTATGAACCA 18615 CATCAAGAACAGTATGAACCA baet45g1214 ACATAACATATTCCGTATACG 18616 ACATAACATAGTCCGTATACG baet45g1214 ATTACACATGCCACATAACAT 18617 ATTACACATGTCACATAACAT basd13j22 CCCTGCGGCAGTTGTTCCTCA 18619 CCCTGCGGCAATTGTTCCTCA baak46e10 GGGCGGCCCAGGTTCCAGGGG 18620 GGGCGGCCCATGTTCCAGGGG baak46e10 CCGACGGAACCAAACAATCAG 18621 CCGACGGAACAAAACAATCAG baak46e10 TAATACACGAGGACACGTGTG 18622 TAATACACGACGACACGTGTG bastE5f0812 AAAAGGGTTATGAAATTACCC 18623 AAAAGGGTTAGGAAATTACCC baak44i12 CGAGCGATCCGAAGGTGTTCT 18632 CGAGCGATCCAAAGGTGTTCT bah21j03 AACGTGTCTCATTCCAGCTTC 18633 AACGTGTCTCGTTCCAGCTTC bah21j03 TCAAGTCCCGAAGCAGAGGAA 18634 TCAAGTCCCGTAGCAGAGGAA bah21j03 TGAGTCTTCAAGACAGAGTGT 18635 TCACTCTTCAGGACAGAGTGT bah21j03 ACCTCTCAGCTTCAAGCATCG 18636 ACCTCTCAGCCTCAAGCATCG

TABLE 23-23 Haruna Nijo SEQ Clones H602 ID NO: kr71b0103 HGCGCGTCCTTGCCGGCAAGG 18637 CGCGCGTCCTCGCCGGCAAGG baak21p23 CTACTTTATCCACATGCTGCG 18639 CTACTTTATCAACATGCTGCG baak21p23 AATACATCAGACCCTGAGCTA 18640 AATACATCAGCCCCTGAGCTA bags6g09 AAAAGGAAAATCAACACATGA 18641 AAAAGGAAAAGCAACACATGA BaSD15M02 GGCCGATAGCAGGGATCCCAG 18642 GGCCGATAGCGGGGATCCCAG basd13f02 ATGCCTGTACATGGACACCAG 18643 ATGCCTGTACTTGGACACCAG basd13f02 AAGCTCATATGAAGTTAATCA 18644 AAGCTCATATAAAGTTAATCA basd13f02 GGTCAAACCATCAAAAAGATT 18645 GGTCAAACCACCAAAAAGATT basd13f02 TTACTCCTTCACAGTGATGCA 18646 TTACTCCTTCGCAGTGATGCA basd13f02 TAACAAGTAACGGTAAAAACT 18647 TAACAAGTAATGGTAAAAACT bah26j10 TCACCATGACTGGTTTAGACT 18650 TCACCATGACAGGTTTAGACT bah26j10 CAGATTCCCAGATGGCTCGTC 18651 CAGATTCCCAAATGGCTCGTC bast65g0113 TTCGTGCCGCGTGATGCAGCC 18652 TTCGTGCCGCCTGATGCAGCC baak4k13 GCTCACTTGCTTTTCAGTGTG 18653 GCTCACTTGCGTTTCAGTGTG baak4k13 GGAGGTCGCCGAACGTCTTGG 18654 GGAGGTCGCCAAACGTCTTGG bags34h11 CATCCCGAAACTTGTCACAGA 18655 CATCCCGAAATTTGTCACAGA bags37j03 CTAACTTATTATTGTTACTAA 18656 CTAACTTATTTTTGTTACTAA bags37j03 CATCCTGACAAAAGAGAGCAA 18657 CATCCTGACAGAAGAGAGCAA bags37j03 TCAAATAGAGCGTGCAGCTAC 18658 TCAAATAGAGTGTGCAGCTAC bags37j03 AGCAAAATCAAGACAGGCAGT 18659 AGCAAAATCAGGACAGGCAGT bah22o08 TGATCTTCTGCCTGTAGAATA 18661 TGATCTTCTGTCTGTAGAATA bah22o08 CTGATAGGTAGGCTGATTCAT 18662 CTGATAGGTAAGCTGATTCAT bah22o08 TCCATAACTTTCCCTTGCACA 18663 TCCATAACTTCCCCTTGCACA bah22o08 TACAGGGTGTGCGAATTCAGA 18664 TACAGGGTGTCCGAATTCAGA bah22o08 GCAACATTTGCGTCACCATCC 18665 GCAACATTTGTGTCACCATCC

TABLE 23-24 Haruna Nijo SEQ Clones H602 ID NO: bags6k13 TGACTGTTACAAGAAATACTG 18666 TGACTGTTACGAGAAATACTG bags6k13 AAATCAGCCAGACACTGACTG 18667 AAATCAGCCACACACTGACTG bags6k13 CCCTCTCTCTCTCTATCGACA 18668 CCCTCTCTCTGTCTATCGACA bags6k13 TTCAGTATGCGAGAATTAACC 18669 TTCAGTATGCAAGAATTAACC bags6k13 GCACCGGCAGTAGCACCTGCA 18670 GCACCGGCAGCAGCACCTGCA bags6k13 ATAGTAACCAGCAGCAGCACC 18671 ATAGTAACCACCAGCAGCACC bastl43C0705 TTGCTGAAACAATTAAAAGTA 18672 TTGCTGAAACGATTAAAAGTA bastl43C0705 ATATGTTGCACATACAAGTGC 18673 ATATGTTGCAGATACAAGTGC bastl43C0705 TACAAGTGCCCACAAATGCTG 18674 TACAAGTGCCGACAAATGCTG bags23h03 TGCTTCTGAACATTTGAGCCC 18676 TGCTTCTGAATATTTGAGCCC bast63B0703 TCAAGTCCATGTGCCTCTGAT 18678 TCAAGTCCATATGCCTCTGAT BaSD13E02 AGAGACTCAGAAAACTAGAAG 18679 AGAGACTCAGGAAACTAGAAG bah13a17 AATCATCAAGTTCGATCAAGG 18681 AATCATCAAGGTCGATCAAGG bah50l23 CTGGCCTCTTTGCTGGCCTGT 18682 CTGGCCTCTTGGCTGGCCTGT bah50l23 TTGCATACGATACTGAAACAT 18683 TTGCATACGAAACTGAAACAT bah50l23 TTTCGACTTTGGCTGACTCTG 18684 TTTCGACTTTTGCTGACTCTG bast73e0210 GGCATTGCGCGGCGGCCAACA 18686 GGCATTGCGCCGCGGCCAACA BaH50O21 TTCCATTTCAGATCTGATGCT 18687 TTCCATTTCAAATCTGATGCT BaH50O21 TTCACTACACTTATTTGAGCA 18688 TTCACTACACATATTTGAGCA BaH50O21 TGGAGTACTAGACTTGGATCT 18689 TGGAGTACTACACTTGGATCT BaH50O21 TTGAGCATGAAGCACTATATT 18690 TTGAGCATGAGGCACTATATT BaH50O21 GCACCCTCCTCTTCCCCGCCA 18691 GCACCCTCCTTTTCCCCGCCA basd21g05 GTTGGCAACATCATAAACTAG 18692 GTTGGCAACAGCATAAACTAG baal5i19 TATAACCCGGGGAGAGATAGA 18694 TATAACCCGGAGAGAGATAGA baal5i19 CACGCACATGGCCCTGTTCAA 18695 CACGCACATGTCCCTGTTCAA

TABLE 23-25 Haruna Nijo Clones H602 SEQ ID NO: baal5i19 ATTATACCTGAAATTCAAGGA 18696 ATTATACCTGTAATTCAAGGA baal5i19 TCTTTGTTGGGATTACACCCA 18697 TCTTTGTTGGAATTACACCCA bah16e04 TTATACCACATGCGGCCCGGG 18698 TTATACCACAGGCGGCCCGGG bah16e04 ACCTGTACCATACAGAGCAGT 18699 ACCTGTACCAAACAGAGCAGT bah16e04 GCACCGTCAGCACAGAAGAAC 18700 GCACCGTCAGTACAGAAGAAC bah16e04 TAGTAGTCATCTCTGGAAATA 18701 TAGTAGTCATGTCTGGAAATA bah16e04 GAAGAACAGAAACTGAATCAG 18702 GAAGAACAGATACTGAATCAG baak32p24 GCACCTTTCTCCGCTGCCCTG 18703 GCACCTTTCTTCGCTGCCCTG baak13d11 ATATACATAGCCAAGAGGTTT 18709 ATATACATAGGCAAGAGGTTT baak13d11 GGGTTGTCATAATTTTATTTG 18710 GGGTTGTCATGATTTTATTTG bah49p10 TTCACCACGGAGCCGTCGACC 18711 TTCACCACGGTGCCGTCGACC bah49p10 ACTGGAGCTGTAGCTCGACCT  18712 ACTGGAGCTGGAGCTCGACCT bah49p10 GCCTGACGCGCCCTTGGAGCC 18713 GCCTGACGCGGCCTTGGAGCC bah49p10 TCCACCAGCGACCCGATGTCC 18714 TCCACCAGCGGCCCGATGTCC baal32n15 TCTATGTCTGATCCAACCTGC 18715 TCTATGTCTGTTCCAACCTGC baal32n15 TCTCGTTCTCATTAGCCAGTT 18716 TCTCGTTCTCGTTAGCCAGTT BaAK22H04 TCCCGGTGCCGTAGGGAGCTT 18718 TCCCGGTGCCATAGGGAGCTT bags15j13 AGGTGGCGCCCGGGCGGGTGT 18719 AGGTGGCGCCGGGGCGGGTGT bags15j13 CGACCTGCGCTTTCGGATTCC 18720 CGACCTGCGCCTTCGGATTCC kr66G0414 AGGACATGTTTCGTATGGTCC  18721 AGGACATGTTCCGTATGGTCC basd22c07 CGCTCTGAAACTGACAGACAG 18722 CGCTCTGAAAATGACAGACAG kr71c1105 TCTAAAACCTTAATCTTATTG 18723 TCTAAAACCTCAATCTTATTG bags34i11 CGGCAAAACCACTCTAAGGTG 18724 CGGCAAAACCGCTCTAAGGTG bags34i11 TCCAAGATTATGACACGGAGT 18725 TCCAAGATTACGACACGGAGT bags34i11 CACATTTATTCTGTACTAGAA 18726 CACATTTATTTTGTACTAGAA

TABLE 23-26 Haruna Nijo Clones H602 SEQ ID NO: bah23k17 CTCATCATCTAGTTCTCTACA 18727 CTCATCATCTGGTTCTCTACA bah23k17 TTTGTTTTCAATCACATTCCA 18728 TTTGTTTTCATTCACATTCCA bah23k17 TGTATCATTGGTACATGGATA 18729 TGTATCATTGATACATGGATA bah23k17 ACAATGTGTCCCTCTGTATCA 18730 ACAATGTGTCTCTCTGTATCA bah23k17 GAAAGTACATAATTTGAGTGT 18731 GAAAGTACATGATTTGAGTGT bah23k17 AAACACTGAAAATAGGGATGA 18732 AAACACTGAAGATAGGGATGA bast39D0107  GGATGCATCAGAGGAGACTCA 18733 GGATGCATCATAGGAGACTCA bast39D0107  TCCTACCAGAAAAGGGACGAT 18734 TCCTACCAGAGAAGGGACGAT bast39D0107 TCTTGGTACAAGGATGCATCA 18735 TCTTGGTACAGGGATGCATCA bast39D0107  ATGTTACTCAAGAGTTGAGAT 18736 ATGTTACTCACGAGTTGAGAT bast39D0107 CCTAGGAGAACTCTTCCTACC 18737 CCTAGGAGAATTCTTCCTACC bast39D0107 AGTATTAGAAAACTGAGAGCT 18738 AGTATTAGAATACTGAGAGCT  BaH44K24 CTGCCTGAGGTGGTGGAATAT 18739 CTGCCTGAGGCGGTGGAATAT BaH44K24 TGCTGGGAATATAGAGGCCAG 18740 TGCTGGGAATGTAGAGGCCAG BaH44K24 TAGAAAATGGTGCCAGAATGC 18741 TAGAAAATGGCGCCAGAATGC baak32k15 TTATAAAGAACATACTTGAGA 18743 TTATAAAGAAGATACTTGAGA baak32k15  TGACATCATAACCACATTAAT 18744 TGACATCATACCCACATTAAT baak32k15 CATGAAGCAATAAAACCAGTT 18745 CATGAAGCAACAAAACCAGTT bah61a13 CCTCGCATAATTATCAACATG 18746 CCTCGCATAACTATCAACATG bah61a13 CTTTGTGCGTCCCGAAACTTT 18747 CTTTGTGCGTACCGAAACTTT bags35n11 TCAGATGTAAATAGGTGTTTT 18748 TCAGATGTAATTAGGTGTTTT bags35n11 CCATACCATCATCAGTTATCA 18749 CCATACCATCGTCAGTTATCA bags35n11 CATCGTCTAATTTGGGGCGGA 18750 CATCGTCTAACTTGGGGCGGA bags35n11 CAGCCTGCAGATGTGTCCATA 18751 CAGCCTGCAGGTGTGTCCATA baak24i03 CAGCCTGCAGATGTGTCCATA 18752 CAGCCTGCAGGTGTGTCCATA

TABLE 23-27 Haruna Nijo Clones H602 SEQ ID NO: baak24i03 CATCGTCTAATTTGGGGCGGA 18753 CATCGTCTAACTTGGGGCGGA baak24i03 CCATACCATCATCAGTTATCA 18754 CCATACCATCGTCAGTTATCA bah28n23 CGTTCTTATGGATTTTACTCC 18755 CGTTCTTATGAATTTTACTCC bah28n23 TTCTTTGCATACTCGGCATCA 18756 TTCTTTGCATGCTCGGCATCA bags10e13 ATGTAACTCAATCCGCATCAT 18757 ATGTAACTCAGTCCGCATCAT bags10e13 TTTGTTTTCAATCACATTCCA 18758 TTTGTTTTCATTCACATTCCA bags10e13 CTCATCATCTAGTTCTCTACA 18759 CTCATCATCTGGTTCTCTACA bags10e13 GAAAGTACATAATTTGAGTGT 18760 GAAAGTACATGATTTGAGTGT bags10e13 GGATGCCTCACGCTTGTCGAT 18761 GGATGCCTCAGGCTTGTCGAT bags10e13 CCATTTTGCATACAGAAACAT 18762 CCATTTTGCAGACAGAAACAT bags19d13 CCTCACCCTCGCCTATTTGTT 18763 CCTCACCCTCACCTATTTGTT bags19d13 GGACGTCGAAGGGCTTCACTT 18764 GGACGTCGAATGGCTTCACTT baak19d04 GCATGAAGTCGTGCCGTGTTA 18765 GCATGAAGTCATGCCGTGTTA baak19d04 CATGATTATAAACAACTTGCC 18766 CATGATTATAGACAACTTGCC BaAL34O19 CGATCTTGACGATCTTCTCCG 18767 CGATCTTGACAATCTTCTCCG BaAK1P04 CGTTCTTATGAATTTTACTCC 18769 CGTTCTTATGGATTTTACTCC BaAK1P04 TTCTTTGCATGCTCGGCATCA 18770 TTCTTTGCATACTCGGCATCA baak35m13 TGAACTCCTCAGCTATCGAGA 18771 TGAACTCCTCGGCTATCGAGA baak35m13 TCGACTGAATTTACTCTGCCA 18772 TCGACTGAATGTACTCTGCCA baak35m13 TGTTAAGACTCCAGAATGACT 18773 TGTTAAGACTTCAGAATGACT baak35m13 CGATAAGTAACTTGCCCGTGA 18774 CGATAAGTAATTTGCCCGTGA bah41n09 TGGCATAGGAGAACACATATA 18776 TGGCATAGGAAAACACATATA bah41n09 CGAAATACCACCAGTGAAGTG 18777 CGAAATACCAACAGTGAAGTG bah41n09 ACGCCTTGTTTGGGAGTCGGC 18778 ACGCCTTGTTGGGGAGTCGGC bah41n09 CACCAGCTCATGACGGACCAA 18779 CACCAGCTCAGGACGGACCAA

TABLE 23-28 Haruna Nijo Clones H602 SEQ ID NO: bah41n09 TTCACTATGAGAGTCATGCAG 18780 TTCACTATGAAAGTCATGCAG BaGS23I12 GGAACCTTACTGACATTACCC 18781 GGAACCTTACCGACATTACCC BaGS23I12 CTCCACCGOCGGCGGGTACCT 18782 CTCCACCGGCAGCGGGTACCT bah16p20 CGACGGTGCCGACCACCGTCA 18783 CGACGGTGCCCACCACCGTCA bah16p20 CCTCCTTGGCAGGGCCGACGG 18784 CCTCCTTGGCCGGGCCGACGG BaH45O16 ACAGGCAGGATAGGACAGCGA 18788 ACAGGCAGGACAGGACAGCGA BaH45O16 CTATCGATGCAGTGCAACTGG 18789 CTATCGATGCGGTGCAACTGG bah21h09 TTGTCTGGTGGCTAGGTAGAT 18790 TTGTCTGGTGTCTAGGTAGAT bah21h09 ATAAAACTGAATTGGAAAAGT 18791 ATAAAACTGATTTGGAAAAGT bah21h09 CAGTTCATCTAATTTTGGTTT 18792 CAGTTCATCTGATTTTGGTTT bah58p22 ACCCGAAATATAGGTGATCCA 18793 ACCCGAAATAGAGGTGATCCA bah58p22 CAGCAACATCAAAGCTAACAT 18794 CAGCAACATCTAAGCTAACAT bags20l19 ACAGGCTACAGCATTTACAGG 18795 ACAGGCTACATCATTTACAGG bah13i10 AGGCTTTCAAAGGCTTGAAGT 18796 AGGCTTTCAAGGGCTTGAAGT bah13i10 TCGATTACTACCTGAGACTGC 18797 TCGATTACTATCTGAGACTGC bah13i10 CTCGGCCTGTCGAATAACCAT 18798 CTCGGCCTGTTGAATAACCAT baal7c15 GGGTCGCTACGAAGGCGAGGT 18801 GGGTCGCTACTAAGGCGAGGT baal7c15 GATCCGGGCATGGGACGATCC 18802 GATCCGGGCACGGGACGATCC bah63f05 GTATGCTATGTTGTGCAACAA 18803 GTATGCTATGCTGTGCAACAA bah63f05 GTTTCCCCTACGACATTATAT 18804 GTTTCCCCTAGGACATTATAT bah63f05 TTCCCCTCGACACCGTATGGA 18805 TTCCCCTCGAAACCGTATGGA bags15j16 GAGCGGAACCGGATATAAGCT 18806 GAGCGGAACCCGATATAAGCT bags15j16 GGCAGATCGATGCGACCGGCT 18807 GGCAGATCGACGCGACCGGCT bags15j16 GTCACGCCGTAAACGACGCGT 18808 GTCACGCCGTCAACGACGCGT bah41e10 GGATGGGCTGCCCGAAGTGGA 18810 GGATGGGCTGGCCGAAGTGGA

TABLE 23-29 Haruna Nijo Clones H602 SEQ ID NO: bah54d08 GTACAGTGCTCAACGGTGTGC 18811 GTACAGTGCTAAACGGTGTGC bah54d08 CCACGTTCAGATTCACCAAGG 18812 CCACGTTCAGCTTCACCAAGG baak18c01 ACTGCGAAGAGTATGAAATTT 18813 ACTGCGAAGAATATGAAATTT baak18c01 AGGAGCCGAGCGAGTTGGGGT 18814 AGGAGCCGAGGGAGTTGGGGT baak18c01 CGTGCACAGATCCAGAGTGGT 18815 CGTGCACAGACCCAGAGTGGT baak18c01 AGTTGGGGTCGGTTCCCCACC 18816 AGTTGGGGTCAGTTCCCCACC baak18c01 ATCTAAAGCTTTACCGGCTCG 18817 ATCTAAAGCTATACCGGCTCG baak18c01 GACATTAGCGATTAGAACCTG 18818 GACATTAGCGGTTAGAACCTG basd21o07 ATGCTGCAGCGAAAATAAACA 18819 ATGCTGCAGCAAAAATAAACA baak43c03 CACACATGTCAGTGGTTATTT 18821 CACACATGTCGGTGGTTATTT baal13f18 GCCGGAAGGTGCTCTTGAATC 18824 GCCGGAAGGTACTCTTGAATC baal13f18 CAACAAGCTCGGCAGCTTCGA 18825 CAACAAGCTCAGCAGCTTCGA baal13f18 ATGTGAGGCACAGGTACATGT 18826 ATGTGAGGCAAAGGTACATGT bags18i22 CTATTACTCACGAGCTAGCGT 18827 CTATTACTCATGAGCTAGCGT bags18i22 AAGATTGAAGATGTCTTGAGA 18828 AAGATTGAAGGTGTCTTGAGA bags9b02 ATGGCATACAGTCTGCGCAAC 18829 ATGGCATACAATCTGCGCAAC bags9b02 TATTTTTCCACAAATAAATAT 18830 TATTTTTCCAAAAATAAATAT bah11e22 CCTGGTTTACCCTGTCATCAT 18831 CCTGGTTTACTCTGTCATCAT bah11e22 GAATGCCTACCTCGATCGTCT 18832 GAATGCCTACATCGATCGTCT bah11e22 GCGCTTACATACGGCGCGGCT 18833 GCGCTTACATGCGGCGCGGCT bah58h09 GACGCCACTGGTGATCTCTTG 18834 GACGCCACTGCTGATCTCTTT baak4c12 GGACGACGACAGCATCGCCGG 18836 GGACGACGACGGCATCGCCGG bags37a05 TTCGGTGTGGCCGATGACAAG 18837 TTCGGTGTGGGCGATGACAAG baak36a20 TTGCTTGAGGAAGAGACGTCT 18840 TTGCTTGAGGCAGAGACGTCT baak36a20 TTTATTATTATAACCAATCCC 18841 TTTATTATTACAACCAATCCC

TABLE 23-30 Haruna Nijo Clones H602 SEQ ID NO: baak36a2O TTACTGAGGCAATGTGTACTT 18842 TTACTGAGGCGATGTGTACTT baak36a2O ACCAATCCCATTTCTTCCTGC 18843 ACCAATCCCAGTTCTTCCTGC baal13mO4 ACCTCCTCCTCCTTGTGCCTG 18847 ACCTCCTCCTGCTTGTGCCTG basl13mO4 TTTCCGTTAATGCCGTCTAAT 18848 TTTCCGTTAACGCCGTCTAAT bags9o24 ACTCAGTCACGCTACACTTTT 18849 ACTCAGTCACACTACACTTTT bah12h16 TGATGCTGCCGCTGCTCCGGT 18851 TGATGCTGCCACTGCTCCGGT bah12h16 GGGGGCCGCAAAGAAGAAGCT 18852 GGGGGCCGCAGAGAAGAAGCT bah12h16 ATGCCTGTTCCTAAGCTGATC 18853 ATGCCTGTTCTTAAGCTGATC baak33n16 GAAACATAATACAGCTACAAA 18854 GAAACATAATTCAGCTACAAA baak33n16 AACGCTGCCGGGGACGCTACC 18855 AACGCTGCCGTGGACGCTACC baak33n16 CACCGAGGGCACACTCGGATA 18856 CACCGAGGGCTCACTCGGATA baak33n16 AGCGGGTTCTTGAGGCCCACA 18857 AGCGGGTTCTCGAGGCCCACA baak33n16 TGAACAGGATCGCCCAGTATC 18858 TGAACAGGATAGCCCAGTATC bah26nO1 CACTCTCAGCGTTCCTTGTGA 18860 CACTCTCAGCATTCCTTGTGA BaGS39EO7 ATGCACGCAGCGAAGAAGAGC 18861 ATGCACGCAGTGAAGAAGAGC BaH33AO9 ATACTATCTAGGGTGCAATCT 18863 ATACTATCTATGGTGCAATCT BaH38AO9 AGAGGCTATCACTTCCGTCGG 18864 AGAGGCTATCGCTTCCGTCGG BaH33AO9 TCTTGTCTTAGGATTGTCTAG 18865 TCTTGTCTTAAGATTGTCTAG

TABLE 24-1 Haruna Nijo Clones H602 SEQ ID NO: BaAL15P01 TGCAAGGCGCATGCCAAAAAG 18866 TGCAAGGCGCCTGCCAAAAAG bags1d06 TATTATATTAGGACTGCTCAT 18867 TATTATATTATGACTGCTCAT bags1d06 AGCATGGCAGGAAGTAGCAGA 18868 AGCATGGCAGCAAGTAGCAGA bast18A0602 CATACTAGTAATGCTTACGGC 18869 CATACTAGTAGTGCTTACGGC bah63h24 TAGAGCGTGTGGCATGCCATC 18872 TAGAGCGTGTAGCATGCCATC BaAK13C16 CTCATGTACAATTAGTACACA 18874 CTCATGTACAGTTAGTACACA BaGS32C19 TGCAGGTCGGAGCACACTGCT 18875 TGCAGGTCGGGGCACACTGCT baak36f08 TTACCGCCGGCGGCAACGTCC 18876 TTACCGCCGGTGGCAACGTCC baak36f08 CCATGGACGGTCCGCAGGACT 18877 CCATGGACGGCCCGCAGGACT baak36f08 GCATGCGGTAACTAGTTACCG 18878 GCATGCGGTACCTAGTTACCG baak36f08 GGGCGTGCATCGGGGCGGGAC 18879 GGGCGTGCATAGGGGCGGGAC baak36f08 CGACCTGGACAGCAGGCAGGT 18880 CGACCTGGACGGCAGGCAGGT baak36f08 TGCAGGTTTTGGGGCACTCGA 18881 TGCAGGTTTTCGGGCACTCGA baak36f08 CGACGTACTGATCCTGGCAGA 18882 CGACGTACTGGTCCTGGCAGA bastl42D1107 TCTCCTATGCCTCGATGTGCA 18884 TCTCCTATGCTTCGATGTGCA bastl42D1107 GCTCTATAAAATTAAATTAGA 10885 GCTCTATAAATTTAAATTAGA baak20g06 CAGAGAAACAAGCAAAAACAG 18889 CAGAAAAACAGGCAAAAACAG baak46o05 GCCGCAACGAATCACAGTCCG 18891 GCCGCAACGATTCACAGTCCG baak46o05 CATCCAATACGTATATATGTT 18892 CATCCAATACTTATATATGTT baak46o05 GAATGCTGCTACAAGCATGTG 18893 GAATGCTGCTCCAAGCATGTG baak46o05 TTTCACATGAATGGTCATACC 18894 TTTCACATGAGTGGTCATACC basd15h22 AGAGAGCGCCCTCCACGATCT 18895 AGAGAGCGCCGTCCACGATCT basd15h22 TCGCTCATCAACTTGTACATG 18896 TCGCTCATCAGCTTGTACATG basd15h22 GCAGTCTAATTAATGAACATC 18897 GCAGTCTAATGAATGAACATC basd15h22 CCGGATCGCCGCACCGGATGT 18898 CCGGATCGCCACACCGGATGT

TABLE 24-2 Haruna Nijo Clones H602 SEQ ID NO: basd15h22 CATCCCAGCTAACGCTCTTGG 18899 CATCCCAGCTCACGCTCTTGG basd19c07 CAACAATCAAACAAACTTTAT 18900 CAACAATCAAGCAAACTTTAT basd19c07 TCAAGGCACCGGCCTTTGGGT 18901 TCAAGGCACCTGCCTTTGGGT baak39a14 TCTCTGATTCTTTCAAATACT 18903 TCTCTGATTCCTTCAAATACT bah31e12 GAGACAGGTCCAGCGATTCTA 18904 GAGACAGGTCGAGCGATTCTA BaSD15L22 ACGCCTTCTTCCTCATGCTCT 18905 ACGCCTTCTTTCTCATGCTCT basd21j11 AACACAGCAGCTCTTTGATTC 18908 AACACAGCAGTTCTTTGATTC BaH48C10 TTCTCTGGAAAGAGGCATATG 18909 TTCTCTGGAACGAGGCATATG BaH48C10 AAATAGAAATTTGGGAGAAAT 18910 AAATAGAAATGTGGGAGAAAT BaH48C10 AGTTATTGCACAATATTAATT 18911 AGTTATTGCATAATATTAATT BaH54J07 TGCCCAATCATACCATCCAAT 18912 TGCCCAATCACACCATCCAAT BaH54J07 ACTCCAGATGCATGCTTGATT 18913 ACTCCAGATGAATGCTTGATT baet46b0903 CCGTGTGCCATGCATTGCCTC 18924 CCGTGTGCCACGCATTGCCTC baet46b0903 TAAGGCGAAGTCACCTAGGGC 18925 TAAGGCGAAGGCACCTAGGGC BaGS19F16 AAAATCATACAGATCCATTCT 18926 AAAATCATACGGATCCATTCT BaGS19F16 AGGCCCACACAGTAACGTCGG 18927 AGGCCCACACCGTAACGTCGG BaGS19F16 AACATTTCAAAAATTCTAAAT 18928 AACATTTCAAGAATTCTAAAT BaGS19F16 ATTCTAAATTCTGTGGTAGAA 18929 ATTCTAAATTTTGTGGTAGAA BaGS19F16 GAGATATCTGTGGAACAACAA 18930 GAGATATCTGCGGAACAACAA baak13g18 CTTGCTTGTCGGCGGTGCTCT 18931 CTTGCTTGTCAGCGGTGCTCT baak13g18 GCCTTGCCAGTGTTTAACCCA 18932 GCCTTGCCAGCGTTTAACCCA bast74h0216 CTTAATTTCACCTATCTGAGC 18934 CTTAATTTCAACTATCTGAGC BaAK45C14 TCTCTTTCATTCTTGTTCTTT 18935 TCTCTTTCATCCTTGTTCTTT kr63f0111 GCATAAATATGAATTACATGC 18937 GCATAAATATTAATTACATGC baak13h18 TGGTGCTAGGGTGCTTGCAGA 18938 TGGTGCTAGGATGCTTGCAGA

TABLE 24-3 Haruna Nijo Clones H602 SEQ ID NO: BaH49B13 GATAAGCATGTACATACACAT 18942 GATAAGCATGCACATACACAT BaH49B13 CAAGCATATAGAACATCAGCA 18943 CAAGCATATACAACATCAGCA BaH49B13 CAAGCTATGGCTTGTGTTCTT 18944 CAAGCTATGGTTTGTGTTCTT BaH49B13 TTACCCAGGACATTGAAATGG 18945 TTACCCAGGAAATTGAAATGG BaGS35A09 TGGTGGCAACAAGCTCCATCA 18946 TGGTGGCAACGAGCTCCATCA bast16a0802 AATTCAAGTGGTTGGAATTAC 18948 AATTCAAGTGATTGGAATTAC basd18k01 GCCGCCACGGTAAGGAAGACG 18949 GCCGCCACGGCAAGGAAGACG BaH58D17 CTGTAGTGCTTCTTCGTCAGG 18950 CTGTAGTGCTCCTTCGTCAGG bags26d01 GGATTGCTTGGACATACCGAG 18953 GGATTGCTTGCACATACCGAG bags26d01 TCTGCTTTGTCGGTGTCATCT 18954 TCTGCTTTGTTGGTGTCATCT bags26d01 AGACCAGTGACGTTCAGCAAT 18955 AGACCAGTGATGTTCAGCAAT bags26d01 CAGAAAGTCGACCATGAGGGA 18956 CAGAAAGTCGTCCATGAGGGA bags26d01 CCTACGCCTTTCCCTTCTTTC 18957 CCTACGCCTTCCCCTTCTTTC bags26d01 GCGGCTCGCTTTGAAGGTGTC 18958 GCGGCTCGCTCTGAAGGTGTC bags26d01 CTGGCTGACTCAGAAAAAGGA 18959 CTGGCTGACTGAGAAAAAGGA bags26d01 CTGAAACCCTAGATGCGGCTC 18960 CTGAAACCCTGGATGCGGCTC basd14k04 ATGCTGCCTAGAACCACTTTG 18961 ATGCTGCCTAAAACCACTTTG basd14k04 TGGCCTAGAAGCATGCTGCCT 18962 TGGCCTAGAATCATGCTGCCT basd14k04 GGCCTTCGACGCTAAGCTTTG 18963 GGCCTTCGACACTAAGCTTTG BaSD24D11 ATATTTGCATGATATCAAGGT 18964 ATATTTGCATCATATCAAGGT BaSD24D11 CGGTCAAGGCTATGAGATGCC 18965 CGGTCAAGGCAATGAGATGCC BaSD24D11 CGAGGTGTTATACTTCATAGC 18966 CGAGGTGTTACACTTCATAGC BaSD24D11 CTTTTCTGACAAAGCGCATGT 18967 CTTTTCTGACCAAGCGCATGT BaSD24D11 GCACGCTTCAAAAGATTAACG 18968 GCACGCTTCACAAGATTAACG BaSD24D11 GTGGTGTTGCCGCAGACTGCG 18969 GTGGTGTTGCGGCAGACTGCG

TABLE 24-4 Haruna Nijo Clones H602 SEQ ID NO: BaSD24D11 GGCAGGAAACGGAACATTGAG 18970 GGCAGGAAACAGAACATTGAG BaH53L10 GCGATCCTACCGTAATGTACT 18971 GCGATCCTACTGTAATGTACT BaH53L10 GACCTTTTGCGCTTCTTTCCA 18972 GACCTTTTGCCCTTCTTTCCA bah57m03 TGGAACAAGTCCAGTAGAAAT 18973 TGGAACAAGTTCAGTAGAAAT bah57m03 GATGAAATAGAGGCGTCGTTA 18974 GATGAAATAGTGGCGTCGTTA bah57m03 ACTTGCGCGATGCGATCGCAT 18975 ACTTGCGCGACGCGATCGCAT bah57m03 CGGATTTCTATCAGACAAGAA 18976 CGGATTTCTACCAGACAAGAA bah57m03 ATTTTTAGAATTAGGTGCAGA 18977 ATTTTTAGAAATAGGTGCAGA bah57m03 TAAGATGAGCATGCCATTTTT 18978 TAAGATGAGCGTGCCATTTTT baal30b10 AAGATTTGGGTTTCTACTTAT 18979 AAGATTTGGGCTTCTACTTAT bah62n16 ACATATATTATTGGCTGCTTT 18980 ACATATATTACTGGCTGCTTT bah62n16 TATGGATCTACGGCGCAGCTT 18981 TATGGATCTATGGCGCAGCTT bah62n16 ATCAGCGTGGCTGCTGATGAC 18982 ATCAGCGTGGTTGCTGATGAC BaGS22F15 CTCCGTACTTTGCAACAAGTT 18983 CTCCGTACTTCGCAACAAGTT BaGS22F15 AGAGGAGTTCACACTGGTAAT 18984 AGAGGAGTTCGCACTGGTAAT BaGS22F15 ACTTTGAGCCGGAAGATTTCG 18985 ACTTTGAGCCAGAAGATTTCG BaGS22F15 TGAATACGAAGGGTGAGATCC 18986 TGAATACGAATGGTGAGATCC BaGS22F15 CACATAGACAGTCTCTTAGAA 18987 CACATAGACATTCTCTTAGAA BaH50J14 CAAGGCTTTGTTAGGTACTAC 18988 CAAGGCTTTGCTAGGTACTAC BaH50J14 CCAAGATGCATGGTCTACTTC 18989 CCAAGATGCACGGTCTACTTC BaH50J14 TATCATTTCATAACCCAAGAT 18990 TATCATTTCACAACCCAAGAT BaH50J14 AGCTCATGGTTGAAGCCGCGA 18991 AGCTCATGGTCGAAGCCGCGA bah19d23 CCACATCTGGAAGGTGTTCAG 18992 CCACATCTGGCAGGTGTTCAG bah19d23 GCCTTCAAGCTTTCATACGCA 18993 GCCTTCAAGCCTTCATACGCA bah19d23 CTGCTGTAGCCTAGCTGAGAC 18994 CTGCTGTAGCTTAGCTGAGAC

TABLE 24-5 Haruna Nijo Clones H602 SEQ ID NO: bags38c06 TGCTGCAAGCAAATTTCTTTT 18995 TGCTGCAAGCCAATTTCTTTT bags38c06 CCCCCTCCTATTTCTTTGTTT 18996 CCCCCTCCTACTTCTTTGTTT bags38c06 GGGAAGCTCAACTGTGGTGGT 18997 GGGAAGCTCAGCTGTGGTGGT BaGS25N05 CGAGCTTGACGACCTGCTTGC 18998 CGAGCTTGACAACCTGCTTGC bastl29B0503 AGGTTCAAGGGCCGGGAGATT  18999 AGGTTCAAGG-CCGGGAGATT 19000 baak43n21 CCCAAAGCTGAATCCACCACA 19002 CCCAAAGCTGGATCCACCACA baak43n21 TTCACCTATTCAGGGAACTAA 19003 TTCACCTATTGAGGGAACTAA baal4a06 ATTTTCTTTCGGCTCCTTATG 19004 ATTTTCTTTCAGCTCCTTATG baal4a06 CGATTTCGATTGCCTTGGGTT 19005 CGATTTCGATCGCCTTGGGTT baal4a06 CGAGGGCACCAAATCTGCAGT 19006 CGAGGGCACCGAATCTGCAGT baal4a06 TCTGTTCCACGTCCATGTCGC 19007 TCTGTTCCACCTCCATGTCGC baal4a06 TTATGATCGCGTTGCCTCGGG 19008 TTATGATCGCCTTGCCTCGGG baal4a06 ATAGCCTGCTCGTTCTGCCTA 19009 ATAGCCTGCTTGTTCTGCCTA baal4a06 CTCCTTCTGGTGATTTTCATT 19010 CTCCTTCTGGAGATTTTCATT baal4a06 CCATGACAGTATCCCTTGTCT 19011 CCATGACAGTCTCCCTTGTCT bast17D1008 CAAACACAAGCAAACTCTTGG 19012 CAAACACAAGTAAACTCTTGG bast17D1008 TACGGCCGGGCACGTTTACTC 19013 TACGGCCGGGTACGTTTACTC BaGS16B17 TCTTGTCGAGGCCTGATGTGT 19014 TCTTGTCGAGACCTGATGTGT BaGS16B17 CTTGATGATGATATAAGGGCC 19015 CTTGATGATGTTATAAGGGCC BaAK16B19 ATCCGCGCTAGCTTTTAGCTA 19018 ATCCGCGCTACCTTTTAGCTA baal40p07 CCATCGAGTAAGGTGAATCGA 19019 CCATCGAGTAGGGTGAATCGA baal40p07 GGCAAAAGAATTGAAGAAACT 19020 GGCAAAAGAACTGAAGAAACT baal40p07 ACCCTGCATGCAAGTCACCGG 19021 ACCCTGCATGTAAGTCACCGG baal40p07 CCATCTTACCGCCTGCATCGA 19022 CCATCTTACCACCTGCATCGA bah49c19 GAACGGTGGTTTATGGTGTTG 19023 GAACGGTGGTGTATGGTGTTG

TABLE 24-6 Haruna Nijo Clones H602 SEQ ID NO: bah49c19 CGAGCTGTTAGAAAATAGCTG 19024 CGAGCTGTTAAAAAATAGCTG bast58C0406 AGACTCGTCAGAAGACGACCA 19025 AGACTCGTCAAAAGACGACCA baak35m24 GCCCGTAAACATCTTGCTGGC 19026 GCCCGTAAACGTCTTGCTGGC bags19h13 TGGGAGGATTGAAAACTTACT 19027 TGGGAGGATTTAAAACTTACT bags19h13 GATTCGTCTCGGATTTGCAGG 19028 GATTCGTCTCAGATTTGCAGG bah57c21 CGACCTTCGCGCCGCCCTTGC 19029 CGACCTTCGCACCGCCCTTGC BaH53P15 ATCTGACAACCTCAAAATCGT 19030 ATCTGACAACTTCAAAATCGT bags20a10 GGAAGAGAAAGCANAACANAG 19031 GGAAGAGAAAACAAAACAAAG bast02d0808 TCACTTTCCGGACAGAAAACA 19032 TCACTTTCCGAACAGAAAACA bags31c04 AACCTTTGTAAACTTGGCACC 19033 AACCTTTGTACACTTGGCACC bah60o22 CCTTCCGACAGATTCAACACC 19035 CCTTCCGACAAATTCAACACC bah60o22 ATTCGCCGTTCACTGCGTCAT 19036 ATTCGCCGTTTACTGCGTCAT bah60o22 AGCATATCTCCTCTTCGGGTC 19037 AGCATATCTCTTCTTCGGGTC BaH50C16 TCTCTGCTCACAAGCTTCATA 19038 TCTCTGCTCATAAGCTTCATA BaH50C16 CGGTACGCCCGAATTTCCTGG 19039 CGGTACGCCCTAATTTCCTGG baak43e04 AAGCTCCTGACGGCGACGCAG 19041 AAGCTCCTGATGGCGACGCAG kr17g1113 TTGTAGCTACCGCCTGCGTGT 19042 TTGTAGCTACGGCCTGCGTGT bags19k19 TTCCATTGTCAGAACATAAGC 19043 TTCCATTGTCGGAACATAAGC baak38b13 GCAGCTTCAGCAGCTCCTCTC 19044 GCAGCTTCAGGAGCTCCTCTC baak38b13 TCAAGAAGGATTATACGTTTA 19045 TCAAGAAGGAGTATACGTTTA baak1d12 ATTATCAAACGGAATAGTCCC 19046 ATTATCAAACAGAATAGTCCC baak1d12 ATTTGGCATGTCTCAGAAATT 19047 ATTTGGCATGCCTCAGAAATT baak1d12 TGCATTCTCTCGTTCGACAGG 19048 TGCATTCTCTTGTTCGACAGG bah28m14 AGTCCCACTCGCTGAGCAGAT 19049 AGTCCCACTCACTGAGCAGAT bah48i15 TAATAATATAGTTAAGCGCAA 19050 TAATAATATAATTAAGCGCAA

TABLE 24-7 Haruna Nijo Clones H602 SEQ ID NO: basd27h23 AGCTCACTCCTTTTGAGCCAC 19051 AGCTCACTCCGTTTGAGCCAC baak21a11 AGCAATAAAAGACGTATTGGC 19052 AGCAATAAAAAACGTATTGGC bast04b0804 TTGCTATGTCCGGCCGATGCT 19053 TTGCTATGTCGGGCCGATGCT bah45f13 TGCGATTTTATATAACGAAAT 19054 TGCGATTTTACATAACGAAAT BaAL8J18 TAATAATATAATTAAGCGCAA 19055 TAATAATATAGTTAAGCGCAA BaGS9D01 CATGTAAAAAGTGACCTCCTG 19056 CATGTAAAAACTGACCTCCTG baal4i06 CTTGCTCTTTTCTTCTTATCT 19058 CTTGCTCTTTCCTTCTTATCT baal4i06 TGAGCTACGAAGGATATTATA 19059 TGAGCTACGAGGGATATTATA baal4i06 CCATCTGCGTGTTCGTGCATC 19060 CCATCTGCGTTTTCGTGCATC bah26i01 CTGTAAAAGAAGATCAAACAG 19061 CTGTAAAAGAGGATCAAACAG bah26i01 CATCATCCTTCGCTCGAGGAA 19062 CATCATCCTTTGCTCGAGGAA bah26i01 CCTCTTCATTCGACAATACAT 19063 CCTCTTCATTTGACAATACAT bah26i01 TACGTTGGGCGGATGACAAGT 19064 TACGTTGGGCAGATGACAAGT bags15c17 GCTGCAACACAAATGGAAAAG 19065 GCTGCAACACCAATGGAAAAG baak1k08 CCGTGGCTTCGCTGTCAAACG 19066 CCGTGGCTTCACTGTCAAACG bags9b03 ACTCCTCAAGCTCGACGATTA 19067 ACTCCTCAAGTTCGACGATTA bags9b03 TAATTATGACCAGAGAGACAA 19068 TAATTATGACGAGAGAGACAA baet42G1214 AGCACCTGCGGGGAGTAAGTG 19069 AGCACCTGCGAGGAGTAAGTG bags22b22 GCATTCATGCCGCTCATCTTT 19071 GCATTCATGCGGCTCATCTTT bags22b22 GACAAACTAGTCATCAAATGC 19072 GACAAACTAGCCATCAAATGC bags22b22 GATGGACACTCGGTAGCAAAA 19073 GATGGACACTTGGTAGCAAAA baal36g05 ACCACCCAGATTATATAATCC 19074 ACCACCCAGAATATATAATCC baal36g05 ACTAGCACTAGCACTAGCACT 19075 ACTAGCACTATCACTAGCACT baal36g05 GATGTATCTACACACGTTTTA 19076 GATGTATCTAGACACGTTTTA baal36g05 TCATGTACGCGAAGAAAACAG 19077 TCATGTACGCAAAGAAAACAG

TABLE 24-8 Haruna Nijo Clones H602 SEQ ID NO: bags33j15 AAAGCAATGTCCGGTTAAGTT 19078 AAAGCAATGTGTGGTTAAGTT bags33j15 CTTGTGGATTCAAGTACATGA 19079 CTTGTGGATTGAAGTACATGA bags33j15 GGGTTCTGGTTGACATACAAA 19080 GGGTTCTGGTGGACATACAAA bast61d0707 TCGAACGCGGCAGGCAACGGC 19082 TCGAACGCGGAAGGCAACGGC basd23a04 GATGATAACTTCATTTGCGCA 19087 GATGATAACTCCATTTGCGCA basd14c15 CACTTACAAAAGACTTCATCA 19088 CACTTACAAAGGACTTCATCA basd14c15 TGTTAACACTCACGACCAGGA 19089 TGTTAACACTTACGACCAGGA bast63c0105 ACTCTACGGAACGGTCGACCT 19090 ACTCTACGGAGCGGTCGACCT bast23c1105 ATGTTTATAACATGATACAGG 19091 ATGTTTATAATATGATACAGG bast23c1105 ACGAGTGTTCTAACGAGTGGT 19092 ACGAGTGTTCCAACGAGTGGT BaAL19H10 TCTGGTACAAATCCTTCAACC 19101 TCTGGTACAAGTCCTTCAACC BaAL19H10 GTTAAATAAAACCTCAAAATT 19102 GTTAAATAAAGCCTCAAAATT BaSD24B15 ACATACAAGAAGCAATAAAAA 19104 ACATACAAGAGGCAATAAAAA BaSD24B15 GTAATCAACTAATTCCTTTGG 19105 GTAATCAACTGATTCCTTTGG bah48n17 GAAAACAGGAACCAAAGGACG 19106 GAAAACAGGAGCCAAAGGACG bah48n17 CAACATTCCGTTTCGAAAGAA 19107 CAACATTCCGCTTCGAAAGAA bags9i05 GCCAACTTGTCGTCGATGTAG 19108 GCCAACTTGTTGTCGATGTAG bags9i05 TCTCTTTTCTGAGCTTCAGAT 19109 TCTCTTTTCTAAGCTTCAGAT bags1l22 GTGTAGCACAAATACAGTTAG 19110 GTGTAGCACATATACAGTTAG bags1l22 TACTGGGCTCACACTGATGAG 19111 TACTGGGCTCCCACTGATGAG bags1l22 AGAAAGTGCTTTACTGGGCTC 19112 AGAAAGTGCTGTACTGGGCTC bags1l22 ACTGCATGGCAAATAATGCAG 19113 ACTGCATGGCGAATAATGCAG bags1l22 TACTGCCGTAAAGTATATTTA 19114 TACTGCCGTACAGTATATTTA bah62p18 CCGATAGGCCACACTTGTCAA 19115 CCGATAGGCCTCACTTGTCAA bah62p18 CAGAATTCTGCTCCTTAGTGC 19116 CAGAATTCTGTTCCTTAGTGC

TABLE 24-9 Haruna Nijo Clones H602 SEQ ID NO: bah62p18 CAGGAGAATACGGAAGTCAGA 19117 CAGGAGAATATGGAAGTCAGA bah55n21 TCGAGGTTTCCACTTTCTAGA 19118 TCGAGGTTTCGACTTTCTAGA bah55n21 ATCATGTACAAATATGTCAGG 19119 ATCATGTACAGATATGTCAGG bah44a05 GTGGACTGTGTAACCCAGTTA 19120 GTGGACTGTGCAACCCAGTTA bah19c13 AATGGTAGTGGTTTGGGAAAA 19121 AATGGTAGTGCTTTGGGAAAA bah19c13 TGTGCTTCAACTTCCTCGTCA 19122 TGTGCTTCAATTTCCTCGTCA bah19c13 GATCATCATAAGCAACAGGCA 19123 GATCATCATACGCAACAGGCA kr28B0703 ATATTGCAGCAGTCATTTACT 19125 ATATTGCAGCGGTCATTTACT kr28B0703 GACCTACCAAGGTTTGCTAAA 19126 GACCTACCAACGTTTGCTAAA kr28B0703 CTTGTATATAGTGACCTACCA 19127 CTTGTATATAATGACCTACCA kr28B0703 TACTCCCTTCCAGTTCAGTTT 19128 TACTCCCTTCTAGTTCAGTTT bastl50C0606 CTTGCAATTAGTAAATGAATG 19129 CTTGCAATTAATAAATGAATG bastl50C0606 AATTTCTGAACGATTCACCTG 19130 AATTTCTGAATGATTCACCTG bah19a05 CAAGAGAAAAGGATCATGTGG 19132 CAAGAGAAAAAGATCATGTGG baal10c06 ATCCGTAGCC-CAAGGATCCT 19133 ATCCGTAGCCACAAGGATCCT 19134 bags19p04 GCATCCCACAGCCGAAGAAGT 19135 GCATCCCACAACCGAAGAAGT baak21a17 TTTTTAACACAATTTTTAACA 19136 TTTTTAACACGATTTTTAACA bah20j14 GATTTAAACACCTCATTTCGT 19137 GATTTAAACAGCTCATTTCGT bah20j14 CATAGCACTCCGATTTCCTTC 19138 CATAGCACTCGGATTTCCTTC baak19a03 AGCTTTAGGGTAAATACATAT 19139 AGCTTTAGGGAAAATACATAT baak19a03 ATTTCCCGTGCTGTCTGAAAC 19140 ATTTCCCGTGATGTCTGAAAC baak19a03 TAACATACAACACCAGAGAAA 19141 TAACATACAAAACCAGAGAAA bags30e19 CGATTTCTGTATACCTTCTTC 19142 CGATTTCTGTGTACCTTCTTC bags11o14 GAACCAGATACCATGGTGTAT 19143 GAACCAGATATCATGGTGTAT bags13p22 TACAGGCAAGTGAACCACCTC 19144 TACAGGCAAGCGAACCACCTC

TABLE 24-10 Haruna Nijo Clones H602 SEQ ID NO: bags13p22 ATCAGCTAGATTTGGCATACA 19145 ATCAGCTAGAGTTGGCATACA kr27A1101 ACGCCTACTCACCGCGCCCCG 19146 ACGCCTACTCCCCGCGCCCCG kr27A1101 ACCCCTCCGCCTCCCGATCTC 19147 ACCCCTCCGCGTCCCGATCTC kr27A1101 CCTAGAGGAGGGAAGGCAAGA 19148 CCTAGAGGAGAGAAGGCAAGA kr27A1101 CCCTCGCCTATAACCCCTCCG 19149 CCCTCGCCTAGAACCCCTCCG bast58f0412 CGGGGCCGCAGATATACATTG 19152 CGGGGCCGCAAATATACATTG bast58f0412 AATGAACCCATAAAGGAGATT 19153 AATGAACCCAGAAAGGAGATT bast58f0412 ACAGGGAACGACTATAAAAGC 19154 ACAGGGAACGGCTATAAAAGC bast58f0412 CCTAACAGTATAAATGCTATG 19155 CCTAACAGTAGAAATGCTATG bast58f0412 TACTACTAACGCGTTACTGGG 19156 TACTACTAACACGTTACTGGG baal32p23 CTCCCTCTGTAAAACTTTCAG 19159 CTCCCTCTGTGAAACTTTCAG baal32p23 GACATAACCACTATTAGTTGC 19160 GACATAACCATTATTAGTTGC baal32p23 CAGAGCTCCTCTGAAGTTTCG 19161 CAGAGCTCCTATGAAGTTTCG basd23m17 CTGCATTAAAAAATTGAGTGT 19162 CTGCATTAAACAATTGAGTGT basd23m17 CATTCGGAACATACCAACCAC 19163 CATTCGGAACGTACCAACCAC bastl30E0509 ACCTGGAATATAAAAACATCG 19164 ACCTGGAATACAAAAACATCG bastl30E0509 TCGCTTCAGAAAGCATCATTA 19165 TCGCTTCAGACAGCATCATTA bah44b08 AGGCCGGGCCGGGCCGGAGAT 19166 AGGCCGGGCCAGGCCGGAGAT bah44b08 CAAAAATCACGGAACAGCAAG 19167 CAAAAATCACAGAACAGCAAG BaGS14N10 GCCTGGGCCAGCGGACGATGA 19168 GCCTGGGCCAACGGACGATGA BaGS14N10 CAGCAGGTGACTTCGGGGACT 19169 CAGCAGGTGATTTCGGGGACT BaGS14N10 GGCCACATTACTCTTGGTGGC 19170 GGCCACATTATTCTTGGTGGC BaGS32B13 ATTTTGCTCCATATGTAGACT 19171 ATTTTGCTCCGTATGTAGACT BaGS32B13 ATAAGTCATTTGCGTAGTTTT 19172 ATAAGTCATTCGCGTAGTTTT BaGS32B13 AGAGAAAATACGGGACGGCAG 19173 AGAGAAAATATGGGACGGCAG

TABLE 24-11 Haruna Nijo Clones H602 SEQ ID NO: BaH30B03 TCAAGCCTGAAAATACGACAA 19174 TCAAGCCTGACAATACGACAA BaH30B03 TTCACATGTTTGCTTTCCTCT 19175 TTCACATGTTCGCTTTCCTCT BaH30B03 GGCCATAAGATATTTCACACC 19176 GGCCATAAGAAATTTCACACC basd11o06 GTAGCACTGACATGGAAGTTC 19178 GTAGCACTGAAATGGAAGTTC basd11o06 TCTGCATACTTTCGATTTGGT 19179 TCTGCATACTGTCGATTTGGT basd11o06 ATAAAGAGGAAATGGTTAGGC 19180 ATAAAGAGGAGATGGTTAGGC basd11o06 GAACGCTCACGAGGACTGAAC 19181 GAACGCTCACAAGGACTGAAC basd11o06 CTCTAGGTGAAAACGTGACTA 19182 CTCTAGGTGAGAACGTGACTA bah17f24 GATCTGTTACATCTACAGCAT 19183 GATCTGTTACGTCTACAGCAT bah17f24 TAACAATCCTCATATTGTGGA 19184 TAACAATCCTTATATTGTGGA bah17f24 AAATACAACAGCATATGAAAA 19185 AAATACAACAACATATGAAAA bah17f24 AAGCCACGCACATAGATCTGT  19186 AAGCCACGCAAATAGATCTGT bah17f24 CTTACAAAATGTAGACAAGCA 19187 CTTACAAAATTTAGACAAGCA BaAK26L17 TCGATCCGGTTCCAAAGAGCT 19188 TCGATCCGGTCCCAAAGAGCT BaAK26L17 GACATGACACTAGGTCCAGCA 19189 GACATGACACCAGGTCCAGCA BaAK26L17 CTCCATCAGCGAGGAACTTCA 19190 CTCCATCAGCAAGGAACTTCA bags23f03 CAGCTATAGTCATGGAAATAA 19191 CAGCTATAGTTATGGAAATAA bags20o06 TTTTCTCCTGTACTCTAGCTG 19192 TTTTCTCCTGCACTCTAGCTG bags20o06 GTGCCGGACATCGCTGTTGTT 19193 GTGCCGGACACCGCTGTTGTT bags19n12 GAAGGCTAGCGTGAAGTTGCA 19194 GAAGGCTAGCATGAAGTTGCA bast14f0612 ATATAGTTTCCCAGCATACTA  19195 ATATAGTTTCACAGCATACTA bags16k13 CTTTCACAGTTTTGATTTGAG 19197 CTTTCACAGTCTTGATTTGAG bags16k13 TGAATCGATGAGTAGTGACTA 19198 TGAATCGATGGGTAGTGACTA baak22c16 GCTCCCTGCAGTCCATATTAA 19199 GCTCCCTGCAATCCATATTAA baak22c16 CATTGCAATCATAGCAAAAAG 19200 CATTGCAATCGTAGCAAAAAG

TABLE 24-12 Haruna Nijo Clones H602 SEQ ID NO: baak22c16 TGAAGACATAATAATCAGCAG 19201 TGAAGACATAGTAATCAGCAG baak22c16 CAGGGCTTGTTAATAGTTTTA 19202 CAGGGCTTGTGAATAGTTTTA baak22c16 ATCTCACTGAAAACAGAGCAT 19203 ATCTCACTGAGAACAGAGCAT baak22c16 GGGAACATGTTCATGCTTCGG 19204 GGGAACATGTACATGCTTCGG baak22c16 TCCGCAGGGCCTGTCTCCCAG 19205 TCCGCAGGGC-TGTCTCCCAG 19206 baak22c16 TGCATCACAGCACGATCAACA 19207 TGCATCACAG-ACGATCAACA 19208 baal40m06 GAATCGTCCTCTTTTGCTGCC 19209 GAATCGTCCTATTTTGCTGCC baal40m06 CCTTTCCTGTCGCCTCCCTTG 19210 CCTTTCCTGTTGCCTCCCTTG bah57a11 ACATCAAAGGATCACTATAAT 19213 ACATCAAAGGGTCACTATAAT bah57a11 TGAAACTGGTGAGGAGATATA 19214 TGAAACTGGTAAGGAGATATA bah41e23 AAACTACCAAGGTTGTATCCA 19215 AAACTACCAAAGTTGTATCCA bah41e23 TGATACAAAAAATGTTTGCAT 19216 TGATACAAAAGATGTTTGCAT bah41e23 CCTGGTATTTTGCTATCAGAA 19217 CCTGGTATTTCGCTATCAGAA BaSD14L18 ACATATGAGTTAGTCCGATGA 19218 ACATATGAGTAAGTCCGATGA BaSD14L18 TTGTCACTCATGAAATACTCA 19219 TTGTCACTCAAGAAATACTCA BaSD14L18 CTCAGCTTCAGTATTTCTGAA 19220 CTCAGCTTCACTATTTCTGAA BaSD14L18 TTTTACGTTTTCCTCAGCTTC 19221 TTTTACGTTTCCCTCAGCTTC BaSD14L18 GCCCAGCAGTGTTCTCCTGAA 19222 GCCCAGCAGTATTCTCCTGAA bah14d17 CCACCCCTCTTCCATCTCCTT 19223 CCACCCCTCTACCATCTCCTT bah14d17 CCATGTCGACGTCGGCCGGCT 19224 CCATGTCGACATCGGCCGGCT bah14d17 CGGCAGCTCGCGGTACCGCTC 19225 CGGCAGCTCGTGGTACCGCTC BaGS1N17 ACACACACACGAAGAGAGAGA 19226 ACACACACACAAAGAGAGAGA BaGS1N17 CTTTGTCAGATGCAGCCACAG 19227 CTTTGTCAGACGCAGCCACAG BaGS1N17 AGAGCTCACATATCCATTTTC 19228 AGAGCTCACAAATCCATTTTC bags27h17 CAGCTCTGAA-GAACCTCGTN 19229 CAGCTCTGAAAGAACCTCGTN 19230

TABLE 24-13 Haruna Nijo Clones H602 SEQ ID NO: bah47p22 GTATCGAATA-GTTAATTAAT 19231 GAATCGAATAAGTTAATTAAT 19232 bah47p22 CCTGTATGTG-AAAATGAAAA 19233 CCTGTATGTGTAAAATGAGAA 19234 bah50a16 GTTGTTTGGGCGTGCAGAGAA 19235 GTTGTTTGGGGGTGCAGAGAA bags7b20 TTAGAACTTAGGCATGCAATG 19236 TTAGAACTTACGCATGCAATG bags7b20 CCTGGTATTTCGCTATCAGAA 19237 CCTGGTATTTTGCTATCAGAA bags7b20 TTGTTTCAGAAAGACTTAGTA 19238 TTGTTTCAGACAGACTTAGTA bags22a02 TGTCTTAGTTGTACATTAATC 19239 TGTCTTAGTTATACATTAATC bags22a02 TATACAGAGAGGGTTTTACTA 19240 TATACAGAGATGGTTTTACTA bags22a02 ATCACACAAATCGTACATGAA 19241 ATCACACAAACCGTACATGAA basd12g02 GGATCAGCAGAGGCAAACAGA 19242 GGATCAGCAGTGGCAAACAGA basd12g02 CAATTAAGTGAAACTGCACCT 19243 CAATTAAGTGGAACTGCACCT basd12g02 GTGCCGAGTATGCCACTTGTA 19244 GTGCCGAGTACGCCACTTGTA basd15o18 AAACGCTTGTGGGCAACCGCC 19245 AAACGCTTGTAGGCAACCGCC basd15o18 TAGCGGAGCCACCGGAATCGT 19246 TAGCGGAGCCGCCGGAATCGT baal25d19 GTTCCTCGTTTGGTTTTGTCG 19247 GTTCCTCGTTCGGTTTTGTCG bast52g0414 AATCGTAATGCAGCACGGATG 19248 AATCGTAATGAAGCACGGATG bags37k06 CACATAGAAACTATCACGCCA 19250 CACATAGAAATTATCACGCCA bags6e22 ACCTGGAGACAACATAGATTA 19251 ACCTGGAGACTACATAGATTA bags6e22 AGATATCTAAAACCTGGAGAC 19252 AGATATCTAACACCTGGAGAC bags6e22 TTTCTCATTATCTGCAAAAAA 19253 TTTCTCATTAGCTGCAAAAAA bags7b06 ATATTTCTGGTTCATGGATTT 19255 ATATTTCTGGGTCATGGATTT bags7b06 GGTCGGGGCCTGGCTGGCTAT 19256 GGTCGGGGCCGGGCTGGCTAT bags7b06 TGGCTATCGAGCTCCGGCGAC 19257 TGGCTATCGAACTCCGGCGAC bags7b06 AGCATGTGCTAGAAATGCCGG 19258 AGCATGTGCTTGAAATGCCGG bast46c0406 CAGACGCCATAACCCATCTGA 19261 CAGACGCCATGACCCATCTGA

TABLE 24-14 Haruna Nijo Clones H602 SEQ ID NO: bast46c0406 TCAAGGTCAATAAGTTTCACA 19262 TCAAGGTCAACAAGTTTCACA bast46c0406 GAGGAATTGGTCAAACGCCAA 19263 GAGGAATTGGACAAACGCCAA bastl45e0509 ACATAGAGGAGAGGATACACC 19264 ACATAGAGGATAGGATACACC bastl45e0509 GGATTTACACGACCGATTTTG 19265 GGATTTACACAACCGATTTTG BaH41G07 CTTCTTCCCCCACATCTGCAT 19267 CTTCTTCCCCTACATCTGCAT bast46H1016 AATTCCACCCTAGAAAATCCA 19268 AATTCCACCCCAGAAAATCCA bah60d12 GAGCCCTCTGTCCTTGGTCCT 19269 GAGCCCTCTGCCCTTGGTCCT bah60d12 CCCTGAGTGCCTTGCCGCCCT 19270 CCCTGAGTGCATTGCCGCCCT bah60d12 TGCCGCCCTCAGACGCCATGA 19271 TGCCGCCCTCGGACGCCATGA bah60d12 CGGCAGCTGCAACCTTCGCCG 19272 CGGCAGCTGCGACCTTCGCCG bah60d12 CCGCGTACAGAGGCCAGCATA 19273 CCGCGTACAGCGGCCAGCATA bah60d12 CTTCATTGTATCCTCTCATCT 19274 CTTCATTGTAGCCTCTCATCT bah60d12 CCCTTAACCACCTCTTCATTG 19275 CCCTTAACCAGCTCTTCATTG bah60d12 TTCCACCTCCTCGCCCTTAAC 19276 TTCCACCTCCACGCCCTTAAC bah60d12 CCTTGAGCGCGTCGGCAGCTG 19277 CCTTGAGCGCCTCGGCAGCTG baal39a19 GTAGGGTCTCCTCTTGGGCAG 19278 GTAGGGTCTCTTCTTGGGCAG baal39a19 TGACGGGGATCGCGGGAGGCC 19279 TGACGGGGATGGCGGGAGGCC baal39a19 GACACTCTGACTCTCAACCGC 19280 GACACTCTGAGTCTCAACCGC baal39a19 TGCCGCTCCGAGCCATCATCA 19281 TGCCGCTCCGCGCCATCATCA baal39a19 TCATCAGGTTCGCGCAGGAGC 19282 TCATCAGGTTTGCGCAGGAGC baal39a19 ACCTCACCTCGGACATGCACG 19283 ACCTCACCTCCGACATGCACG baal39a19 GGGCAGGTCCGCCAGGCACCT 19284 GGGCAGGTCCTCCAGGCACCT baal39a19 TGCACGCGAACGACTGGGACT 19285 TGCACGCGAAGGACTGGGACT bast14e0909 GACACACAAAAATAATGGTAC 19287 GACACACAAAGATAATGGTAC baet13g0713 CGTCGCCGGCCGGCTTGATGC 19288 CGTCGCCGGCTGGCTTGATGC

TABLE 24-15 Haruna Nijo Clones H602 SEQ ID NO: baak13c05 GGAGGACGTGCAGATCATCCC 19289 GGAGGACGTGGAGATCATCCC bastl41g0513 ACTTGCTCAGGGAGGAGTGGT 19290 ACTTGCTCAGCGAGGAGTGGT baal19b12 TAGTGCATCTTTTGCAATGGC 19292 TAGTGCATCTCTTGCAATGGC baal19b12 TTACATGATAGGGTCATTACC 19293 TTACATGATAAGGTCATTACC baal19b12 TAGAGTATCAGATGGAAATAT 19294 TAGAGTATCAAATGGAAATAT baal19b12 TTCATGAGTTTACAATTATTT 19295 TTCATGAGTTCACAATTATTT baal19b12 ATAGAATCCAGAGTGTCAACT 19296 ATAGAATCCAAAGTGTCAACT baal19b12 TGGAAATATTGTAAATGACGA 19297 TGGAAATATTATAAATGACGA baal19b12 TGACGAAATAGAGTACACAGC 19298 TGACGAAATAAAGTACACAGC baal39b05 CCTGGTCACCTCCACCCTGCG 19299 CCTGGTCACCGCCACCCTGCG baal1j03 CAACCTACGCTGTGGCTATCA 19300 CAACCTACGCCGTGGCTATCA BaGS33N15 TAAAGAAGGGAAAAATTAACG 19301 TAAAGAAGGGGAAAATTAACG basd11k09 CCTGGTCACCTCCACCCTGCG 19302 CCTGGTCACCGCCACCCTGCG basd11k09 GACGGCCTTAACTTCTTCACC 19303 GACGGCCTTACCTTCTTCACC baet43H0416 GAACTAGCCACAGGTTGCACT 19304 GAACTAGCCATAGGTTGCACT bah11m06 CAGATAGTACCTCGATCCTCT 19305 CAGATAGTACTTCGATCCTCT bah11m06 GAATAAGGAACGTTCTTAGAA 19306 GAATAAGGAATGTTCTTAGAA bags27h20 ATTAGCATCGTTTTAGACAAG 19310 ATTAGCATCGGTTTAGACAAG basd27c09 GAACTAGCCACAGGTTGCACT 19311 GAACTAGCCATAGGTTGCACT basd27c09 TCAACACTTTTGTATAGAAGG 19312 TCAACACTTTGGTATAGAAGG basd27c09 TCTCCAGGTGGTTCTGCATGA 19313 TCTCCAGGTGTTTCTGCATGA baal25o01 TTAACATAATTAGAGGATTGA 19315 TTAACATAATCAGAGGATTGA baal25o01 AAACAACTAGGTAGTGAAAAC 19316 AAACAACTAGATAGTGAAAAC baal25o01 TTATCACTGCCCCAGATCCTT 19317 TTATCACTGCACCAGATCCTT BaAK19J09 GGGCTTTGTTCTCCACTCAGG 19319 GGGCTTTGTTTTCCACTCAGG

TABLE 24-16 Haruna Nijo Clones H602 SEQ ID NO: BaAK19J09 CCATTTTGACGTATGTTTGTG 19320 CCATTTTGACATATGTTTGTG bags20n14 CCGTCATGACGGATATACAGG 19321 CCGTCATGACAGATATACAGG baak31k16 AGAATTGAGCAGCGACTCTAA 19322 AGAATTGAGCGGCGACTCTAA baak31k16 CCATCCCCACCGTTTCTTTCA 19323 CCATCCCCACTGTTTCTTTCA baak31k16 CGTAGTAGGCGTGCGTCCGGT 19324 CGTAGTAGGCNTGCGTCCGGT bah42o12 TAGCGTGAGTTATGAAGTTAT 19325 TAGCGTGAGTGATGAAGTTAT bags4p14 TGATAGTTCATATACAAAACA 19326 TGATAGTTCAGATACAAAACA bags4p14 CCTGTGTGCAACCCAAAAACA 19327 CCTGTGTGCAGCCCAAAAACA bags4p14 ATAAAATTTGAACTAACGTAG 19328 ATAAAATTTGTACTAACGTAG bags4p14 GCTAGAGAAGCCAACAAGTAG 19329 GCTAGAGAAGTCAACAAGTAG BaH62B09 AATCCCTTGCCCAAGGCTTGA 19331 AATCCCTTGCTCAAGGCTTGA BaH62B09 ATATGTAGAAAGAGCATGACA 19332 ATATGTAGAAGGAGCATGACA BaH62B09 GAAATCCAAAGCCCTAGCCCA 19333 GAAATCCAAAACCCTAGCCCA bags19j10 CCTTCCTGCTGGGGTCTTCAT 19334 CCTTCCTGCTCGGGTCTTCAT bags15k18 CAAACCAGCAGTGGGAAGCGA 19336 CAAACCAGCAATGGGAAGCGA bags15k18 GTTGACAAACAGTATAGCGTG 19337 GTTGACAAACGGTATAGCGTG bags15k18 AACACACTGTGCGCTGAATAC 19338 AACACACTGTACGCTGAATAC bags15k18 TCCCTATAATCAGTATGAAAC 19339 TCCCTATAATGAGTATGAAAC basd26c09 ACTCCAAGAGCGTCCGCCTTC 19342 ACTCCAAGAGTGTCCGCCTTC basd26c09 ATGGAAAAGAGCAATGTCGAC 19343 ATGGAAAAGAACAATGTCGAC basd26c09 CCATGTGGCTCACCATGGAAA 19344 CCATGTGGCTTACCATGGAAA basd20b11 TCCGGACGGAAGGAGTACTAC 19345 TCCGGACGGAGGGAGTACTAC basd20b11 CCTAGAAATGAATGTATCTAG 19346 CCTAGAAATGGATGTATCTAG BaH46F11 CATTTTCTATCTGAAATCTTG 19347 CATTTTCTATTTGAAATCTTG BaH46F11 AAACACACACACGCAACCCAA 19348 AAACACACACCCGCAACCCAA

TABLE 24-17 Haruna Nijo Clones H602 SEQ ID NO: BaH46F11 ACTTCAGGAATACCGAGTTCT 19349 ACTTCAGGAACACCGAGTTCT bah30f03 GGTTGTAAACGTCATGTGTCT 19351 GGTTGTAAACATCATGTGTCT baak33i12 ATAAAGTTCTGGAAACAATGG 19354 ATAAAGTTCTTGAAACAATGG bags37a16 TTCCTGGCCGCCGGCGCCCTC 19356 TTCCTGGCCGGCGGCGCCCTC bags37a16 TGGCGATGCCGCTGCCGCGCT 19357 TGGCGATGCCCCTGCCGCGCT bags39p06 TCGGCCCATTCCCGCAGGGCC 19358 TCGGCCCATTTCCGCAGGGCC baal12n06 TCAGCAGTTTCCATCCCTCAC 19359 TCAGCAGTTTTCATCCCTCAC bags9a03 GGCAATCTCAAGGACGAGAGG 19360 GGCAATCTCAGGGACGAGAGG bags9a03 AGGGTCGTCCCGGTTAGGGTC 19361 AGGGTCGTCCGGGTTAGGGTC bags9a03 GCAGCGAGCACATTGAAAGCA 19362 GCAGCGAGCAGATTGAAAGCA bags9a03 CATACTTCTGGGTCCAGCTGC 19363 CATACTTCTGCGTCCAGCTGC bags9a03 TGCGGGCTGTTGTCTCGTACT 19364 TGCGGGCTGTCGTCTCGTACT baet24F1212  GTTTCCACAATGCAAGGCTAC 19365 GTTTCCACAACGCAAGGCTAC baak29d10 AGCATTGTCAGTTCTTGGACG 19366 AGCATTGTCAATTCTTGGACG bags13i12 CCGTGGAAGCTTGTCGGGTCC 19367 CCGTGGAAGCCTGTCGGGTCC baal16a23 CCTGGAAGCCGTGGAGGTTCA 19368 CCTGGAAGCCATGGAGGTTCA baal16a23 CAGGCTGAAGCGTGTGCGAGG 19369 CAGGCTGAAGTGTGTGCGAGG bastl29F0711 GGCCCAGAGAGGAAGATTTCA 19370 GGCCCAGAGAAGAAGATTTCA bastl29F0711 GCTTGGCCGCCTCTCCCCTGC 19371 GCTTGGCCGCGTCTCCCCTGC bastl29F0711 CTCTTCAGCAGGCTCTGCAGC 19372 CTCTTCAGCAAGCTCTGCAGC bastl29F0711 CGTACAGTAGCCATACTGTGA 19373 CGTACAGTAGTCATACTGTGA bastl16b1204 TTTTGGACGACCAGATTTGTG 19374 TTTTGGACGAGCAGATTTGTG baal15e10 GATTCATGACGAAATTCCTTC 19376 GATTCATGACAAAATTCCTTC baal33m18 CACCATCAGACGTGCTGCCTG 19378 CACCATCAGATGTGCTGCCTG baak24e23 TAATGTCAGAAATCATGAGAA 19379 TAATGTCAGACATCATGAGAA

TABLE 24-18 Haruna Nijo Clones H602 SEQ ID NO: baak24e23 CCACACTGCTCAAGGGACTCC 19380 CCACACTGCTAAAGGGACTCC baak24e23 AAAAATGTTCGCAAGGATGTT 19381 AAAAATGTTCTCAAGGATGTT baak24e23 CAGAAACAAGTCATTATGGTC 19382 CAGAAACAAGACATTATGGTC baak24e23 ATTTGCAATAGTTAGTATATC 19383 ATTTGCAATAATTAGTATATC baak24e23 ACACGCTACTCATAGTCGACA 19384 ACACGCTACTTATAGTCGACA baak24e23 AACTCCCACAGGTATAAATGA 19385 AACTCCCACATGTATAAATGA baak24e23 TGATGGTCAGTGGATAATAAT 19386 TGATGGTCAGCGGATAATAAT baak46j13 TCATACACATAGTCGCACAAA 19387 TCATACACATGGTCGCACAAA baak46j13 CGAGGAGGGTAAAGAGGATGA 19388 CGAGGAGGGTGAAGAGGATGA bah49o05 GTTCATTGATAGTGTAAAATT 19389 GTTCATTGATCGTGTAAAATT bah49o05 CAAGGTGCGGTGGACATCAGC 19390 CAAGGTGCGGAGGACATCAGC bah49o05 CTGTCTGCCGCCGGACATGAA 19391 CTGTCTGCCGTCGGACATGAA bah49o05 GACCATCAGAAATGGAGTGTT 19392 GACCATCAGATATGGAGTGTT baal15e05 TGTTATCGTCGCCCTCGTATG 19393 TGTTATCGTCCCCCTCGTATG BaGS30P02 CCTCGCTCTCAAACTGCACGA 19394 CCTCGCTCTCGAACTGCACGA BaGS30P02 GATCCCTCGAGCTTGCTCGCA 19395 GATCCCTCGATCTTGCTCGCA baak1o08 CCTAACCACAGAAATAAATAA 19396 CCTAACCACACAAATAAATAA BaSD15D05 TTTTTGACCCTGCACTCTCCT 19397 TTTTTGACCCCGCACTCTCCT bags38n23 ACGCAAATGCTATCGCCAATT 19399 ACGCAAATGCCATCGCCAATT bags38n23 ACTGATGCACCTGACCCTTAA 19400 ACTGATGCACTTGACCCTTAA bags38n23 AATCTCTCGACCAGTGACCTG 19401 AATCTCTCGAGCAGTGACCTG bags38n23 GCCTTGGATGGGATTTATCTC 19402 GCCTTGGATGAGATTTATCTC bags38n23 AGGAACTCCTCCAGGTAAGCT 19403 AGGAACTCCTTCAGGTAAGCT baak36a14 CTATGAATCACGCTCATAACA 19404 CTATGAATCATGCTCATAACA baak36a14 TCCTAATAGCGGCAGCAACAA 19405 TCCTAATAGCAGCAGCAACAA

TABLE 24-19 Haruna Nijo Clones H602 SEQ ID NO: bags35n24 GGTCGAGCGTGATGCGATGCA 19410 GGTCGAGCGTCATGCGATGCA bags35n24 GCCATGAGAAGGAGCTCGAGG 19411 GCCATGAGAAAGAGCTCGAGG bah37j21 AGTACTTGTCTTCACTCGCCT 19412 AGTACTTGTCCTCACTCGCCT bah31e13 ATCATCAAATCGTTTACTATA 19413 ATCATCAAATTGTTTACTATA bah31e13 TGAAGCACACGATGTGGCAGT 19414 TGAAGCACACAATGTGGCAGT bah31e13 TCCGACTTATACAGCGGAAAG 19415 TCCGACTTATGCAGCGGAAAG bah31e13 CTACACCGCCTTTTATTCTCT 19416 CTACACCGCCATTTATTCTCT baal30l05 AATACCTATCCACTTGTTGTA  19417 AATACCTATCAACTTGTTGTA baal30l05 TGTATAATGACACAATGCATG 19418 TGTATAATGAGACAATGCATG baal30l05 TAGACCCAAGTGAGCCAAAAG 19419 TAGACCCAAGCGAGCCAAAAG baal30l05 GATCGTATATAAAATAAAACA 19420 GATCGTATATCAAATAAAACA bags38o10 CCAGTGCAGATGTCTCAAGGA 19421 CCAGTGCAGAGGTCTCAAGGA bags38o10 CTTTCTCAGAAAATGCTTGGC 19422 CTTTCTCAGAGAATGCTTGGC bags38o10 CGTTAATAGTGCTTTCCTTGC 19423 CGTTAATAGTCCTTTCCTTGC bastl30A0701  CTCCTCCCCTCTTTACACCTA 19425 CTCCTCCCCTTTTTACACCTA bah49l21 AGCACTTCACCTTCTCCCGCG 19426 AGCACTTCACTTTCTCCCGCG bah49l21 TGCACTAACTGTTCCTGTGAA 19427 TGCACTAACTATTCCTGTGAA bags19l03 GACAACATAGTAAAATAGTAT 19428 GACAACATAGCAAAATAGTAT bags19l03 AACAAAAAAGGAAAATGAACT 19429 AACAAAAAAGAAAAATGAACT bags19l03 TAACCAAGTCATCTAGAACAA 19430 TAACCAAGTCGTCTAGAACAA baak44p03 AGGAGGAGGAGGAAGAAGAAG 19431 AGGAGGAGGAAGAAGAAGAAG kr10h0216 CCATAATCCTGCAAGAATTCC 19433 CCATAATCCTCCAAGAATTCC kr10h0216 CGTCCCGGCGCCCCAGATCCA 19434 CGTCCCGGCGTCCCAGATCCA bah11i21 ATGTACATATGGTTCGTGTAT 19436 ATGTACATATAGTTCGTGTAT bah11i21 TTTTAGCCGTGAGAAAAGAAT 19437 TTTTAGCCGTAAGAAAAGAAT

TABLE 24-20 Haruna Nijo Clones H602 SEQ ID NO: bah11i21 AATTTCATGTCTTCTTTCCCG 19438 AATTTCATGTTTTCTTTCCCG baak20k23 GGAATTTATGCGGCTGCGCTA 19439 GGAATTTATGTGGCTGCGCTA baak20k23 CATCCTGCAAGCAGTGTTGAC 19440 CATCCTGCAAACAGTGTTGAC basd14n22 ACGACTGAGATCTTGTGTACC 19443 ACGACTGAGACCTTGTGTACC basd14n22 ATTTGCAGCACACAGAGAGCA 19444 ATTTGCAGCATACAGAGAGCA basd14n22 ACAGAGTTCAGGGCCGATATC 19445 ACAGAGTTCACGGCCGATATC basd14n22 TATTAGATGTGCGGTTTGATG 19446 TATTAGATGTTCGGTTTGATG basd14n22 CGCGGCAGAATGGGCACGACT 19447 CGCGGCAGAAAGGGCACGACT basd14n22 TGTCGGGGACTATGAGGGGCA 19448 TGTCGGGGACGATGAGGGGCA basd14n22 TAGTAGACCAACAGGAGGTTG 19449 TAGTAGACCAGCAGGAGGTTG basd14n22 AAAGCCAGAGAAACAAATAAT 19450 AAAGCCAGAGCAACAAATAAT baet44D0707 CGCTTGCTGGTGGCAGCTTGC 19451 CGCTTGCTGGCGGCAGCTTGC bah16p10 CATTCATCAGGGAATGCACAG 19452 CATTCATCAGCGAATGCACAG bah16p10 TGTGTCACCCGCAACCTCCAG 19453 TGTGTCACCCTCAACCTCCAG baak42j01 CCAGCTGGACCCAGTGCTTAG 19455 CCAGCTGGACACAGTGCTTAG baak42j01 ACAAATAGACACATGATTTAG 19456 ACAAATAGACCCATGATTTAG BaAK38O08 TGGTGCTAAAGAAGTGTTTGC 19457 TGGTGCTAAAAAAGTGTTTGC baal15f24 ATAACTTTTAGCTGCTTTCAG 19461 ATAACTTTTATCTGCTTTCAG baal15f24 TCTCATATCTCTAGTCTCGAG 19462 TCTCATATCTGTAGTCTCGAG baal15f24 AACACCTGAAACCACTTTTCT 19463 AACACCTGAAGCCACTTTTCT baal15f24 AGGTGCTGATGGTGTTTTTTG 19464 AGGTGCTGATAGTGTTTTTTG baal15f24 CTCTTTTGCTTGAGCAATTAG 19465 CTCTTTTGCTAGAGCAATTAG baal15f24 TCTAACAACAAATAGATACGT 19466 TCTAACAACATATAGATACGT baal15f24 GATGAATTTAGATAAATGACA 19467 GATGAATTTACATAAATGACA baal15f24 GCAATTAGCTCTGCTTGCATC 19468 GCAATTAGCTGTGCTTGCATC

TABLE 24-21 Haruna Nijo Clones H602 SEQ. ID NO: basd18b21 TGGCATAGCATGTCAGAGGTC 19469 TGGCATAGCAAGTCAGAGGTC basd18b21 GGCGGATCATCGGTATGCATC 19470 GGCGGATCATTGGTATGCATC basd18b21 TCAGAGGTCAACCATAACACA 19471 TCAGAGGTCATCCATAACACA basd17g23 GCAGCTCATTGAGCTCCGTCT 19472 GCAGCTCATTTAGCTCCGTCT basd17g23  TGGTCTTCTTCCGCAGCTCAT 19473 TGGTCTTCTTTCGCAGCTCAT basd17g23 GCCTGGCGACGCAGACATGTC 19474 GCCTGGCGACACAGACATGTC baak41k22 TCATGTTAAACTCACGGTTCA 19475 TCATGTTAAATTCACGGTTCA baak41k22 GTTCAGGAAAAAGCACAAAGT 19476 GTTCAGGAAATAGCACAAAGT baak41k22 ATACACCAATTTGAACCTACT 19477 ATACACCAATGTGAACCTACT BaSD16B18 GGTGGAAACAGTTCACGGTTA 19478 GGTGGAAACAATTCACGGTTA BaSD16B18 GCTGATTTTACCATACATGAT 19479 GCTGATTTTATCATACATGAT BaSD16B18 TACATGATATCTGTGAAGTAC 19480 TACATGATATGTGTGAAGTAC BaSD16B18 CCATCTGATCTGCAGCACCCG 19481 CCATCTGATCAGCAGCACCCG BaSD16B18 TGCAGACTGGGCAGCTAAACT 19482 TGCAGACTGGACAGCTAAACT BaSD16B18 TGTGGCCACAGATGGTTGATG 19483 TGTGGCCACATATGGTTGATG baak21o03 CTTCAGTACTGAAGTTAAACA 19484 CTTCAGTACTTAAGTTAAACA baak21o03 GCATGCTGGACTACTACTACT 19485 GCATGCTGGAATACTACTACT bast79C1105 AGTCTGTTTTTATTCAGGTAG 19486 AGTCTGTTTTCATTCAGGTAG BaAL37H08 CGGAAATTACTTCCTGGATAC 19487 CGGAAATTACCTCCTGGATAC baak4j17 AGACCCCCATCGGGGCCGCCA 19488 AGACCCCCATTGGGGCCGCCA baak4j17 GCGACGCCGACGCGGAGTAGC 19489 GCGACGCCGAGGCGGAGTAGC bags11i15 TCACCAGACTCGCAAGCCTGT 19491 TCACCAGACTTGCAAGCCTGT bags11i15 GAGGGGTCGCCACATGGACAT 19492 GAGGGGTCGCGACATGGACAT bags11i15 ATGAGGGGTCACAACGTGGAC 19493 ATGAGGGGTCGCAACGTGGAC baak35b18 TATGCAAGTTTTTGGCTGAAA 19495 TATGCAAGTTATTGGCTGAAA

TABLE 24-22 Haruna Nijo Clones H602 SEQ ID NO: baak35b18 GCTGCTACCAAATCAGCTACT 19496 GCTGCTACCAGATCAGCTACT baak35b18 TGGTACTTAACGCCCCTGAGG 19497 TGGTACTTAAAGCCCCTGAGG baak35b18 GCTTTTACTGCCCTTCAGGCC 19498 GCTTTTACTGTCCTTCAGGCC baak35b18 CAGAGAAGTGAAAAAAAAAAG 19499 CAGAGAAGTGGAAAAAAAAAG baak35b18 ACTACAAAGCTTATGGTACTT 19500 ACTACAAAGCATATGGTACTT baak41o03 GCAGGTGACAAAACTGGCAGT 19501 GCAGGTGACAGAACTGACAGT baak41o03 GACAAAACTGGCAGTAACAAC 19502 GACAGAACTGACAGTAACAAC baal1b16 ATGCAGTGCTCAATTCCAAGC 19504 ATGCAGTGCTAAATTCCAAGC baalb16 AGATGCAGGTAAACTTCATGC 19505 AGATGCAGGTCAACTTCATGC baalb16 AGTACAAGACGGAAAACGGAA 19506 AGTACAAGACAGAAAACGGAA baalb16 TTTCATCTATATTACCTTCCA 19507 TTTCATCTATGTTACCTTCCA baalb16 AGCTTCGAGCAAAGAACACAT 19508 AGCTTCGAGCTAAGAACACAT bags30n17 GGGCATCCTCAAGCCTTGCCC 19511 GGGCATCCTCGAGCCTTGCCC bags30n17 TTGCCCTGGAATCCTCAAGCT 19512 TTGCCCTGGAGTCCTCAAGCT bags5b16 GCTTCGACAAAGACACAACAT 19514 GCTTCGACAACGACACAACAT baal5f06 ACTTGCCATTCTCTTGGACAT 19515 ACTTGCCATTTTCTTGGACAT bah42g19 TGGTAAAACAACTGAGACACA 19516 TGGTAAAACATCTGAGACACA bah42g19 GGTCGTTAGCTATGCAGGAAC 19517 GGTCGTTAGCCATGCAGGAAC bah42g19 TCGACCAAGTTACCTTCACTG 19518 TCGACCAAGTCACCTTCACTG BaH47O14 CGTACCGTGCCTGATACCCCG 19519 CGTACCGTGCTTGATACCCCG BaH47O14 TGTGGATGGATAATAACACGA 19520 TGTGGATGGAGAATAACACGA BaH47O14 TGCCATCCTGCATTGTTGAGC 19521 TGCCATCCTGTATTGTTGAGC BaH47O14 TGTTGAATTACTGACCTTATT 19522 TGTTGAATTATTGACCTTATT BaH47O14 AACACGAGGAATTTTTGTTGT 19523 AACACGAGGATTTTTTGTTGT BaH12L06 TTGGTATGGACGTCAGGATGC 19524 TTGGTATGGATGTCAGGATGC

TABLE 24-23 Haruna Nijo Clones H602 SEQ ID NO: BaH12L06 ATGGGATGGCATCGGCGAAGG 19525 ATGGGATGGCGTCGGCGAAGG bah51a21 GTGCATCCCAATCGGTAGTGT 19528 GTGCATCCCAGTCGGTAGTGT bah51a21 CTTCCCTTTGGTTTAGCAATA 19529 CTTCCCTTTGTTTTAGCAATA basd12p12 TACATAACTTATTTACATGGG 19532 TACATAACTTCTTTACATGGG bags19l10 TTGCCTCAATCGACGGGAACA 19538 TTGCCTCAATTGACGGGAACA BaH56J21 GCGAGCCGCGGGTATGCCTTG 19539 GCGAGCCGCGAGTATGCCTTG BaH56J21 AAACAACCTTTGATAAAGTAT 19540 AAACAACCTTCGATAAAGTAT BaH56J21 GCAGGAACCAGGTGATGAACG 19541 GCAGGAACCACGTGATGAACG BaH56J21 GTGACACTACGATCATCGACA 19542 GTGACACTACAATCATCGACA bags16i19 GCAGGCATGTGTTACGGCACT 19544 GCAGGCATGTTTTACGGCACT bags16i19 CGCTGCTGCGGCCAGAGCATC 19545 CGCTGCTGCGCCCAGAGCATC bags16i19 GCATCCTGAGCGAGGCAGGCC 19546 GCATCCTGAGAGAGGCAGGCC bah54h01 AGCTGGATGCTTATGTGCACC 19548 AGCTGGATGCATATGTGCACC bah54h01 TAATAATTCAAAGCAAATCAA 19549 TAATAATTCAGAGCAAATCAA bags20e19 CCATTCCGGCGACACAAGCAT 19550 CCATTCCGGCAACACAAGCAT bags20e19 TACTCAACAAAGTCGATGGCC 19551 TACTCAACAAGGTCGATGGCC bags20e19 GAACCTTGTCCCTTCAGCCAG 19552 GAACCTTGTCACTTCAGCCAG bags20e19 CCTTTCTCGAGGTCCGGCAGC 19553 CCTTTCTCGACGTCCGGCAGC bast74c0206 TGAACAGCCCAAAGTTCTTCT 19554 TGAACAGCCCGAAGTTCTTCT bast74c0206 TTATTCGACCACGAAATATCC 19555 TTATTCGACCGCGAAATATCC bast74c0206 GCGTGCCATTGCCGACGTGGT 19556 GCGTGCCATTCCCGACGTGGT bags20i01 GTGTGCTCGAAGGCTGTATCT 19557 GTGTGCTCGAGGGCTGTATCT bags20i01 GAAAAGAGCAATCTATCAAGG 19558 GAAAAGAGCAGTCTATCAAGG baak13l10 TGGACAGACCATTATCGCTTC 19559 TGGACAGACCGTTATCGCTTC baak42g21 CAGTAGAGGC-ATCACTTATG 19560 CAGTAGAGGCCATCACTTATG 19561

TABLE 24-24 Haruna Nijo Clones H602 SEQ ID NO: baak42g21 CTAGATTTGCGAATATAGCGC 19562 CTAGATTTGCAAATATAGCGC basd19n14 TAAAGGATAGTTGCAGCTGCA 19563 TAAAGGATAGCTGCAGCTGCA basd19n14 TACAAGCATATCTTTTGTTTA 19564 TACAAGCATACCTTTTGTTTA basd19n14 GACCCACAAATTTGACAGACA 19565 GACCCACAAACTTGACAGACA basd19n14 TGTAGTGGTACCATCACAACT 19566 TGTAGTGGTAGCATCACAACT basd19n14 ATGCTTCATCACACGGTAATG 19567 ATGCTTCATCGCACGGTAATG BaAK22K17 TTGCTACGTACGTACGCGTAT 19570 TTGCTACGTATGTACGCGTAT baal6o24 GAGTTTGCTCATAGGGACGTA 19571 GAGTTTGCTCGTAGGGACGTA baal6o24 TCATAAGCCAAAACCAGTTGT 19572 TCATAAGCCAGAACCAGTTGT baal6o24 TTTGCATCGCGTCACATGAAA 19573 TTTGCATCGCATCACATGAAA BaH22C09 CAAAGGCCAATAATGAAATGA 19574 CAAAGGCCAACAATGAAATGA baal24n12 GCGCGCGACTTATAACTTGTT 19577 GCGCGCGACTAATAACTTGTT baal24n12 TGATACATTACGCAAAGAAAT 19578 TGATACATTATGCAAAGAAAT BaSD16I09 TATGATATACATGTGCGAGAG 19579 TATGATATACGTGTGCGAGAG BaSD16I09 CTGGCTCCTCGGTGATGGTAA 19580 CTGGCTCCTCAGTGATGGTAA BaSD16I09 TACATCCACCGCAGGACCAAA 19581 TACATCCACCCCAGGACCAAA BaAL13B22 CATCAGGTTGGAATATGCCGG 19582 CATCAGGTTGAACTATGCCGG BaAL13B22 TTGACATGGTCGGTCGATCGT 19583 TTGACATGGTTGGTCGATCGT BaAL13B22 TTGAACTAATGCAGAAATATA 19504 TTGAACTAATACAGAAATATA bastl30f0111 GCATGCCGGGGCCTTAACACC 19586 GCATGCCGGGACCTTAACACC baak40c12 ACAGCAGAGTGGCAATTACAT 19587 ACAGCAGAGTCGCAATTACAT baak40c12 AAGATAGCCCTTTGAAAAAAG 19588 AAGATAGCCCCTTGAAAAAAG baak40c12 CGTCTAGCTTTGTCAGAATGA 19589 CGTCTAGCTTCGTCAGAATGA baak40c12 CCCCTTCGCCTACACCAACAA 19590 CCCCTTCGCCGACACCAACAA bah15l04 ATGTGCAATTGCGGTGCATCG 19591 ATGTGCAATTCCGGTGCATCG

TABLE 24-25 Haruna Nijo SEQ ID Clones H602 NO: baak31e03 TTGTCCGACTACATAAGGAAA 19593 TTGTCCGACTGCATAAGGAAA baak31e03 CTCCCTCGCAACCGCTCATCC 19594 CTCCCTCGCACCCGCTCATCC baak31e03 ACAGAACATTATCCTCCCTCG 19595 ACAGAACATTTTCCTCCCTCG baak31e03 TGATTAAGATTCATCTAAATT 19596 TGATTAAGATCCATCTAAATT bah57d15 AATTCTGCTGCGTTCCAGTTA 19597 AATTCTGCTGTGTTCCAGTTA baal13o01 CTACTGATATAATTAAACTAA 19598 CTACTGATATGATTAAACTAA baal13o01 TGAGGCCACAGGGTTATTATT 19599 TGAGGCCACATGGTTATTATT baal13o01 ACCAGAACAGAGCTTAGTTTA 19600 ACCAGAACAGGGCTTAGTTTA bags20f18 TCTAGGCTAGCATACAACAAT 19601 CTTATTCTAG-ATACAACAAT 19602 bags20f18 AACAATTGGCTACAAATAACG 19603 AACAATTGGC-ACAAATAACA 19604 basd26p04 AGAACTAGCCATGCAAGTTTC 19605 AGAACTAGCCGTGCAAGTTTT basd26p04 TTGTCAAAACCAAAAACAGAT 19606 TTGTCAAAACTAAAAACACAT basd26p04 ACCAAAAACAGATGATGAATG 19607 ACTAAAAACACATGATGAATG basd26p04 CGCATCATGAAAATTTTGCTA 19608 CGCATCATGACAAGTTTGCTA basd26p04 ATCATGAAAATTTTGCTAAGA 19609 ATCATGACAAGTTTGCTAAGA basd26p04 TGATAGCTGAATAACTGTCAG 19610 TGATAGCTGAGTTACTGTCAG basd26p04 TATCTTATTCTAACCTCATAA 19611 TATGTTATTCAAACCTCATAA basd26p04 CTGCAGATATCTTATTCTAAC 19612 CTGCAGATATGTTATTCAAAC basd26p04 TAAATCAGTGAAATGGGAATA 19613 TAAATCGGTGGAATGGGAATA BaH42J22 ATTTGGCCAGACGAACAACCG 19614 ATTTGGCCAGGCGAACAACCG BaH42J22 CGCCCCTCTCGACTGCATCCC 19615 CGCCCCTCTCAACTGCATCCC BaH42J22 TCATGTGTTTTCCTATGATCC 19616 TCATGTGTTTGCCTATGATCC kr30b1103 TCCCACCAGGCATCGCTCCCT 19618 TCCCACCAGGTATCGCTCCCT kr30b1103 TTCCCAACTTCTAATTTGCAT 19619 TTCCCAACTTATAATTTGCAT kr30b1103 CTGCAATGTCTTTGCACCACC 19620 CTGCAATGTCGTTGCACCACC

TABLE 24-26 Haruna Nijo SEQ ID Clones H602 NO: kr30b1103 AGGTCTATGTAATCTTTGTTT  19621 AGGTCTATGTTATCTTTGTTT baal4e21 CTCCATTTTTTTTAATCAACT 19624 CTCCATTTTTCTTAATCAACT baal4e21 TCTACCACAATCCAGAATATC 19625 TCTACCACAACCCAGAATATC BaGS29D05 GAAGATAACACCATCTTAGAA  19626 GAAGATAACAACATCTTAGAA BaGS29D05 TTAGAAAACGCTGTGACGTCA 19627 TTAGAAAACGATGTGACGTCA BaGS29D05 GTGACGTCATCTAGGGTCAGT 19628 GTGACGTCATTTAGGGTCAGT bah54d24 TGCATCAAGACTCATCCAATT 19629 TGCATCAAGAATCATCCAATT bah54d24 GGCTCTGTGATTCTGCATCAA 19630 GGCTCTGTGACTCTGCATCAA bah54d24 TCCGGGGCGATGTTGGCCGCG 19631 TCCGGGGCGACGTTGGCCGCG bah54d24 GGGGGCATCCAGGGGCTCCGG 19632 GGGGGCATCCTGGGGCTCCGG bags38h17 AACCGGTTTTCCGAGGCTTCA 19635 AACCGGTTTTGCGAGGCTTCA baak30b23 CGTCCTCCGATGGCAGGAAGT 19636 CGTCCTCCGACGGCAGGAAGT baak30b23 TGGCCTCGTATGCGTCCTCCG 19637 TGGCCTCGTAGGCGTCCTCCG baak30b23 GGTAGATAACGCTCTTGACAC 19638 GGTAGATAACTCTCTTGACAC baak24p09 CTCCCTCTATTAACTAATATA 19640 CTCCCTCTATAAACTAATATA baak24p09 GCAGCTAAACTGGCTAGGCAT 19641 GCAGCTAAACAGGCTAGGCAT baak24p09 TATGCTTGCAGACCCTCTGCA 19642 TATGCTTGCACACCCTCTGCA BaGS22I06 TGTAAATAAATTTTGTAATAA 19643 TGTAAATAAACTTTGTAATAA BaGS22I06 CACTACAAAATTATGATGCTA 19644 CACTACAAAACTATGATGCTA BaAL34P18 AAATCCCCACGAAAAACTTAT 19645 AAATCCCCACTAAAAACTTAT BaAL34P18 TCTCGCCGACGTAGTACTCGT 19646 TCTCGCCGACATAGTACTCGT BaAL34P18 AACTTATTCATCTCTTGAACC 19647 AACTTATTCAGCTCTTGAACC bah12e02 GGTTTGCTCCTACTGCTACTT 19650 GGTTTGCTCCCACTGCTACTT BaAL15F23 GGATGGTCAGCATTTCTGTGC 19651 GGATGGTCAGTATTTCTGTGC BaAL15F23 TCATGTCATTCCACTGATGAC 19652 TCATGTCATTACACTGATGAC

TABLE 24-27 Haruna Nijo Clones H602 SEQ ID NO: BaGS25O15 TGCAATAGCTGAAATTGTGAC 19653 TGCAATAGCTAAAATTGTGAC BaGS25O15 ATGGGTTGGTCGGGATGCTCG 19654 ATGGGTTGGTTGGGATGCTCG bags33m02 GCATATAATCAACACTCAACA 19657 GCATATAATCGACACTCAACA bags33m02 GAGTAGTTTCGATGCGAGAGT 19658 GAGTAGTTTCAATGCGAGAGT bags27p13 GCTATTTTGCCGTTCTCAACA 19659 GCTATTTTGCTGTTCTCAACA bags27p13 TCAACAGAGTATAAACGATAA 19660 TCAACAGACTGTAAACGATAA bags27p13 AAACCCATCTTCATACATCAG 19661 AAACCCATCTCCATACATCAG bah48g21 TGTAGTTGACTAAGCAAAGAT 19662 TGTAGTTGACGAAGCAAAGAT bah48g21 AAGGCGGCGGAACGATCATGG 19663 AAGGCGGCGGGACGATCATGG

TABLE 25-1 Haruna Nijo SEQ ID Clones H602 NO: bah14a22 CTCGGACAGCATCCACGGCTT 19664 CTCGGACAGCGTCCACGGCTT bah14a22 CTTCAAGGGGTTCGACGCCGT 19665 CTTCAAGGGGCTCGACGCCGT BaGS32P17 TGGAGTGGATCGACACCATCC 19667 TGGAGTGGATAGACACCATCC BaGS32P17 GAGAACACAGGCATAGAACAC 19668 GAGAACACAGCCATAGAACAC BaGS32P17 CCCTCTCCATAAGGTCCTTGA 19669 CCCTCTCCATCAGGTCCTTGA BaGS32P17 ACACCATCCTGATGAGCCCCC 19670 ACACCATCCTAATGAGCCCCC baak35b06 TCAATTTTGCGAAACCCGCCT 19671 TCAATTTTGCAAAACCCGCCT basd21f17 AGGAGGAGGATTGAAGAGGAG 19672 AGGAGGAGGACTGAAGAGGAG basd21f17 CCCTTTCCCTCTCCCTCTCCC 19673 CCCTTTCCCTTTCCCTCTCCC basd21f17 AGAATCCGACAATGGATGCTG 19674 AGAATCCGACGATGGATGCTG baal22h02 TCTTCTTCTTCTCCTTCTTCT 19679 TCTTCTTCTTGTCCTTCTTCT bah56g24 ATCTCTCCTCGCATACTTGCT 19680 ATCTCTCCTCACATACTTGCT bah56g24 CATTGGCCGGCGGCGGGTTCA 19681 CATTGGCCGGTGGCGGGTTCA bah56g24 CGCGCACGTCGGCAATCAGCG 19682 CGCGCACGTCAGCAATCAGCG bah56g24 CTTCCGCCTCTGGGGAGACCT 19683 CTTCCGCCTCCGGGGAGACCT baak32f06 CGAACGACGGTTTCACGCGAG 19688 CGAACGACGGCTTCACGCGAG basd21d13 CATCATCACACGGCGCACTCA 19690 CATCATCACAAGGCGCACTCA basd21d13 TACAGCTAAAGTAAAATAGTT 19691 TACAGCTAAAATAAAATAGTT bast46d0408 CGAAAAGGATGGATCGATAAC 19692 CGAAAAGGATAGATCGATAAC bast55B1204 TCTACTCCTACTTTGTTTTTA 19693 TCTACTCCTAGTTTGTTTTTA bast55B1204 ACCTTGGCAATGATGTCCTCC 19694 ACCTTGGCAACGATGTCCTCC bast55B1204 GATAACAATGTCAGTTCAGTT 19695 GATAACAATGCCAGTTCAGTT bast55B1204 GTGAATTCTGTCCAAAGATGC 19696 GTGAATTCTGCCCAAAGATGC bags39b15 CTTAATGACAGTAAGGGTTAT 19697 CTTAATGACACTAAGGGTTAT bags39b15 GAAAACCTAAAAATTGCATGC 19698 GAAAACCTAACAATTGCATGC

TABLE 25-2 Haruna Nijo SEQ ID Clones H602 NO: bah29o22 GTAGATATCCTCTTCCCCGCC 19701 GTAGATATCCCCTTCCCCGCC bah29o22 TGCAGCAGAGGCGACGGCTCA 19702 TGCAGCAGAGCCGACGGCTCA baet31e1010 TCTATTGCATGGTTGTGCAAA 19703 TCTATTGCATCGTTGTGCAAA baal39h14 AGATCAAAACTGCTTTATTTA 19704 AGATCAAAACCGCTTTATTTA baal39h14 TACATGAAGAAACACAGTGAT 19705 TACATGAAGACACACAGTGAT baal18m18 CTGCAAAGACGAAACAGTCTC 19706 CTGCAAAGACAAAACAGTCTC baal18m18 TATCAAAATTTTCTTTTGAGA 19707 TATCAAAATTGTCTTTTGAGA baal18m18 CGATCATCAGGCTGTCAAGAA 19708 CGATCATCAGACTGTCAAGAA basd24e02 AGGCTTGCTAGAATCCGCCGG 19709 AGGCTTGCTACAATCCGCCGG basd24e02 AAAGTAAAGGTGTCAGCGAGT 19710 AAAGTAAAGGCGTCAGCGAGT basd24e02 AGAGGATGACGACGAGCTTCA 19711 AGAGGATGACCACGAGCTTCA baak11d04 AGTCAAGGCTTGCCGAGGATT 19714 AGTCAAGGCT-GCCGAGGATT 19715 baal33m06 TTAAGGTGAANGGCAATAGAT 19719 TTAAGGTGAAAGGCAATAGAT baal33m06 TTGTACCTGGCTCGTGAGCAA 19720 TTGTACCTGGTTCGTGAGCAA bastl50E0309 GCTCCGGGTCGTGGTTCATGC 19721 GCTCCGGGTCATGGTTCATGC bastl50E0309 CGTGGCGAACACCCTTTATTC 19722 CGTGGCGAACGCCCTTTATTC bags20i17 GAATCTTGACATAGGGCAACA 19723 GAATCTTGACGTAGGGCAACA bags20i17 GCCACCATGCGTGTCCCTGCG 19724 GCCACCATGCATGTCCCTGCG BaGS22A07 ATATACACACCCTGATGCAAA 19725 ATATACACACACTGATGCAAA BaGS22A07 GCACAAAAAACGTGGATGGAA 19726 GCACAAAAAATGTGGATGGAA bah41b09 CTCCCAGCCTGTGCCCCCGGC 19727 CTCCCAGCCTCTGCCCCCGGC bah41b09 CCCTTCTTGGGCTTCTTGCTA 19728 CCCTTCTTGGCCTTCTTGCTA bah41b09 TGCCGAACCCGAGCTCGTCGG 19729 TGCCGAACCCAAGCTCGTCGG bah41b09 CCCCCCTCGGGCTTGCCCTTC 19730 CCCCCCTCGGCCTTGCCCTTC bah41b09 GTCGATCTCGATGCTCTCCTT 19731 GTCGATCTCGTTGCTCTCCTT

TABLE 25-3 Haruna Nijo SEQ ID Clones H602 NO: bah41b09 TGTCGGACTTGGTGGCCTCGA 19732 TGTCGGACTTAGTGGCCTCGA bah41b09 CCCCCGGCCTGCTTCTTGCTC 19733 CCCCCGGCCTCCTTCTTGCTC BaGS19O23 CGACCGTGCCGTCTTTCGAGC 19735 TGACCGTGCCATCTTTCGAGC BaGS19O23 GCTTGGGTGCCGCCAGAAGCG 19736 GCTTGGGTGCTGCCAGAAGTG bags14h12 CGACGGGGAATCATTTGAAGG 19740 CGACGGGGAAACATTTGAAGG bags14h12 CTTCCTTTGGTTTGGTTCAAG 19741 CTTCCTTTGGATTGGTTCAAG bags14h12 ACAAGAGTCAAAACTGCATCC 19742 ACAAGAGTCAGAACTGCATCC bah32e15 GGGCACGAGACGTCCTTTTGG 19743 GGGCACGAGATGTCCTTTTGG bah32e15 TGCACTGCTGTTGCACCTGCC 19744 TGCACTGCTGCTGCACCTGCC bah32e15 CAGCCTCGCAAAGTGCTCGGC 19745 CAGCCTCGCATAGTGCTCGGC bags39e16 TGTATAGCTTTATGAGGATAG 19747 TGTATAGCTTCATGAGGATAG bags39e16 TTTTGAGTGGTTGGCATAGTT 19748 TTTTGAGTGGCTGGCATAGTT bags39e16 CAGGAAATAGTTTTTGAGGGG 19749 CAGGAAATAGGTTTTGAGGGG baal32m10 ATCAGTGGATCTTATCCCTGA 19752 ATCAGTGGATGTTATCCCTGA baal32m10 CCTTGTTTAATTATCTTTTAG 19753 CCTTGTTTAAGTATCTTTTAG baal32m10 CTTTTAGGGAGTGACAATTTG 19754 CTTTTAGGGATTGACAATTTG baal32m10 AAAAAATTAGCTCAGTATTGC 19755 AAAAAATTAGTTCAGTATTGC basd12p12 ATGCACATAGTGCATGTATTA 19756 ATGCACATAGGGCATGTATTA basd12p12 CGGCAAGATCGGCATCTATTT 19757 CGGCAAGATCAGCATCTATTT basd12p12 CTATTTGCAACTTACAAATCA 19758 CTATTTGCAAGTTACAAATCA basd12p12 CAGATGTATATCAGCAGCAAT 19759 CAGATGTATAGCAGCAGCAAT baal39e15 GTGCGTGTGCATGCGCCGGAG 19760 GTGCGTGTGCGTGCGCCGGAG baal39e15 CCAAACCGTAAAGTGGTAACC 19761 CCAAACCGTAGAGTGGTAACC BaGS31B01 AGCACGATGCCGTAATGTTTA 19763 AGCACGATGCTGTAATGTTTA BaGS31B01 ACTCAGCCTTCGCAATCCACA 19764 ACTCAGCCTTTGCAATCCACA

TABLE 25-4 Haruna Nijo SEQ ID Clones H602 NO: BaH48L11 CTGCTTTCAAGTTTCGCTGCT 19767 CTGCTTTCAAATTTCGCTGCT BaH48L11 CAAATTTATACTCCCAGCCAA 19768 CAAATTTATATTCCCAGCCAA bah56c09 TCAGTGAAGCCCATGAACTTC 19770 TCAGTGAAGCTCATGAACTTC bags20k09 GAAGTGCGGGGCGGCATCAGC 19771 GAAGTGCGGGACGGCATCAGC bags20k09 GGCCCTTTCAAGGGGAAGTGC 19772 GGCCCTTTCAGGGGGAAGTGC bags20k09 TGTGGTCGTCGGGCGCCAGGC 19773 TGTGGTCGTCAGGCGCCAGGC bags20k09 GAGTTACATTGCAACACAGCT 19774 GAGTTACATTTCAACACAGCT basd25b08 TCGCCGCCTGAGACGACGCTG 19775 TCGCCGCCTGGGACGACGCTG basd25b08 TGGGGCACCAGTCGACGAACT 19776 TGGGGCACCAATCGACGAACT basd25b08 GCACACGGCCATCTGGACCTT 19777 GCACACGGCCCTCTGGACCTT basd25b08 TGCCCTCGCCGACGTACCAGT 19778 TGCCCTCGCCCACGTACCAGT basd25b08 CGTCGCCCTCGTCACCGTCCT 19779 CGTCGCCCTCATCACCGTCCT basd25b08 TGAGACAGCAGGCCATGTACT 19780 TGAGACAGCATGCCATGTACT bast27e0309 CAAGCTGCTTGGGCCAACCAG 19781 CAAGCTGCTTCGGCCAACCAG baet20D0107 AAATTTACAATACACTACAGG 19782 AAATTTACAACACACTACAGG baet20D0107 GCAGAGAAAAACAATAATGAG 19783 GCAGAGAAAACCAATAATGAG baet20D0107 AACGAGCAGAATTGCATTCTA 19784 AACGAGCAGAGTTGCATTCTA baak26n10 CTTCTTCTTTCGGATAAGAAA 19790 CTTCTTCTTTGGGATAAGAAA baak26n10 GTGTGCCTTTCAGTGTGTAGT 19791 GTGTGCCTTTNAGTGTGTAGT baak26n10 TATATTTTAG-CCTTCATCAT 19792 TATATTTTAGGCCTTCATCAT 19793 bast22b0204 CTTCGTTGCCGGCCGGCCAGC 19794 CTTCGTTGCCAGCCGGCCAGC baal3c05 TTTTAGGACCGCAACATCTCG 19795 TTTTAGGACCACAACATCTCG baal3c05 GTGACGCTGAAGATTCGCTCT 19796 GTGACGCTGACGATTCGCTCT BaAK20B09 CCATCTGTTGTTCCCTCCATT 19798 CCATCTGTTGCTCCCTCCATT BaAK37H01 AAAATGAAAAATTGAAAGCTC 19799 AAAATGAAAATTTGAAAGCTC

TABLE 25-5 Haruna Nijo SEQ ID Clones H602 NO: BaAK37H01 CGGCCTGGTCATGGATTCGGG 19800 CGGCCTGGTCTTGGATTCGGG BaAK37H01 GAAAGCTCTCACTGGAATTGT 19801 GAAAGCTCTCGCTGGAATTGT BaGS33P13 GTAGCCCAACTCATGCTACTA 19802 GTAGCCCAACGCATGCTACTA BaGS33P13 TGCCCCTTATGATCTTGAAGT 19803 TGCCCCTTATAATCTTGAAGT bags18h09 GGGATGGACATCAAACAATAA 19806 GGGATGGACACCAAACAATAA baal9m23 CCTGTTTCCTCGACAGGAAAT 19808 CCTGTTTCCTTGACAGGAAAT baak17d18 TCTTTTGCTTCGGGTTGGGAC 19814 TCTTTTGCTTTGGGTTGGGAC bags13a12 CAAGTGTTGGAGATATATGGT 19815 CAAGTGTTGGTGATATATGGT bags13a12 TTCCAGCCTCAAAGTACATGT 19816 TTCCAGCCTCGAAGTACATGT kr34f0212 AATTACGGCCCATCCATGTGG 19818 AATTACGGCCTATCCATGTGG kr34f0212 GTGAAATTGTATTACTTTACT 19819 GTGAAATTGTCTTACTTTACT kr34f0212 AACGGAGGGACTATTTAATAT 19820 AACGGAGGGAGTATTTAATAT kr34f0212 CACGACAAGTATTTTGGAACG 19821 CACGACAAGTGTTTTGGAACG kr34f0212 AATTCATTTAAAAGAAGAAGA 19822 AATTCATTTACAAGAAGAAGA baal16d11 GCTTGCTGTTGCTAACTAGCA 19823 GCTTGCTGTTCCTAACTAGCA baal16d11 CGAGAGCAACAGAAGGTATGT 19824 CGAGAGCAACCGAAGGTATGT baal16d11 ATCCATCATCGAGAAATTTAC 19825 ATCCATCATCAAGAAATTTAC bah22d04 CATAAGGTTCGTCGAAGTATT 19826 CATAAGGTTCATCGAAGTATT bah22d04 GCGAGGCGACGAATGCGAGTG 19827 GCGAGGCGACAAATGCGAGTG bah22d04 CCTGCCACGCTCTGAGGAACA 19828 CCTGCCACGCCCTGAGGAACA BaGS6O07 ATGGCCATAGCAGCAGCAGCA 19830 ATGGCCATAGTAGCAGCAGCA BaH37O10 GACATTGAACGCGAGGTTTGT 19831 GACATTGAACACGAGGTTTGT baal9i11 GGCACGGGCTTGTAATTCTGG 19834 GGCACGGGCTCGTAATTCTGG BaAL39NO6 CCCGAGTAACCATCCCTTGAC 19835 CCCGAGTAACTATCCCTTGAC BaAL19I19 CTATCTGTACGTGATTTTCTT 19836 CTATCTGTACATGATTTTCTT

TABLE 25-6 Haruna Nijo SEQ ID Clones H602 NO: BaAL19I19 TGCCAGGTGCATTATACCACA 19837 TGCCAGGTGCTTTATACCACA basd12I11 GCAGTTACTATGAAGCTTAGG 19838 GCAGTTACTACGAAGCTTAGG bastl46D1008 AAACAACTAGTGTAAAGGAGA 19839 AAACAACTAGCGTAAAGGAGA BaH50H16 TTGTCAGGCAAAGTAATATTA 19840 TTGTCAGGCAGAGTAATATTA bags9n05 GCTTCAGGTCCTCTTGCTCTG 19841 GCTTCAGGTCATCTTGCTCTG bags9n05 ATGAGAGACCAAATATTTGCT 19842 ATGAGAGACCGAATATTTGCT kr61b1103 AACCTTTATTTATATTATGTG 19843 AACCTTTATT-ATATTATGTG 19844 kr27b0103 CTGCACTGCA-TCGCGCAATG 19845 CTGCACTGCAGTCGCGCAATG 19846 kr33h1115 CCCAAATGGCGGCGTCGGCGG 19847 CCCAAATGGCCGCGTCGGCGG baak30l02 GAGCAGCAGAGATTGTGCATG 19848 GAGCAGCAGACATTGTGCATG baak30l02 CACTCAGATGCTGTCCTCGTC 19849 CACTCAGATGTTGTCCTCGTC bah55a12 GTTCCAGAAGGACACAATGAT 19850 GTTCCAGAAGTACACAATGAT bah55a12 CACGGGGCCGACATATTCACC 19851 CACGGGGCCGCCATATTCACC bah55a12 AAGTAAATGTCATCTTTTTCT 19852 AAGTAAATGTTATCTTTTTCT bah26k14 TAAAACCTGAAAACCACTAAT 19853 TAAAACCTGAGAACCACTAAT bah26k14 ATGTCCATGTACATCAATAGG 19854 ATGTCCATGTGCATCAATAGG bah26k14 ATTTATCTAGAAATGGATATA 19855 ATTTATCTAGCAATGGATATA basd18m17 AAGACTGGGTATTATCCATAT 19857 AAGACTGGGTGTTATCCATAT BaH53B03 GAAGGCTGAGACGAAGCTGTC 19859 GAAGGCTGAGGCGAAGCTGTC bags14d19 ATGAGCACGTATTAGGTGGTA 19864 ATGAGCACGTCTTAGGTGGTA bags14d19 TGCTGAGCTATATGTTGCACA 19865 TGCTGAGCTACATGTTGCACA baal20f05 TACCTCCTTCCGCTCCTTTCT 19866 -ACCTCCTTCGGCTCCTTTCT 19867 baal20f05 ACTGATGATCCTTGGG-AAAG 19868 ACTGATGATCTTTGGGGAAAG 19869 BaH18H12 TTGGAGTTATGAGCCAGAGGA 19870 TTGGAGTTATAAGCCAGAGGA baal9o21 GTGTGTATCCACCGTCAGTTC 19871 GTGTGTATCCTCCGTCAGTTC

TABLE 25-7 Haruna Nijo SEQ ID Clones H602 NO: baal9o21 GCGTGCCACCGACGTTGAGGC 19872 GCGTGCCACCTACGTTGAGGC baal9o21 TTGAGGCTGGTGAATCCATCT 19873 TTGAGGCTGGCGAATCCATCT bags21a02 ACTTCATCAGGGCTACGTCGA 19874 ACTTCATCAGCGCTACGTCGA BaAL3G19 GAAATTCTGCTACAGACCACA 19875 GAAATTCTGCCACAGACCACA baal7a09 CACTTGGACAATTCTATGCAA 19876 CACTTGGACACTTCTATGCAA baal7a09 ATCTAGTATAGGTTTGGATGC 19877 ATCTAGTATAAGTTTGGATGC baal7a09 CTGTCTTCGCAGCAGATGCGA 19878 CTGTCTTCGCGGCAGATGCGA bast70b0804 GACCGGTGATCCGTCCGCCTC 19879 GACCGGTGATGCGTCCGCCTC bastl14f0412 CCAACGACTACGCAAATTATA 19881 CCAACGACTAGGCAAATTATA baal3f19 TGCCTCCTTTCCCCGATAGGC 19883 TGCCTCCTTTTCCCGATAGGC baal3f19 TCGTATCAGCGATAACTATGC 19884 TCGTATCAGCAATAACTATGC baal3f19 GTATGTGGATCTTCGTATCAG 19885 GTATGTGGATATTCGTATCAG baal3f19 ACAGGTCATCTAGACGAAGCA 19886 ACAGGTCATCAAGACGAAGCA baal3f19 AATCCCTGGCAACCTTGATGT 19887 AATCCCTGGCTACCTTGATGT baal1e10 TCTTCTGAGTCGGGAATTTCA 19888 TCTTCTGAGTGGGGAATTTCA baal1e10 GACTTGGATCGACAGCAGCTT 19889 GACTTGGATCAACAGCAGCTT bags6k02 CAGAAGACCCCATGTGGGTTC 19890 CAGAAGACCCGATGTGGGTTC kr42h0315 ATCAATAGTCTTAATATTGTA 19891 ATCAATAGTCCTAATATTGTA kr42h0315 GTGAGTAACCTCTCTTCTCTC 19892 GTGAGTAACCGCTCTTCTCTC bah39p02 AAGGTCCAGACCATGCCCTGT 19893 AAGGTCCAGAGCATGCCCTGT bah39p02 AAGCAGTGAATTCTCTCTCAT 19894 AAGCAGTGAAATCTCTCTCAT BaGS9L14 ATGATGTCTCCGCAATCTCAT 19895 ATGATGTCTCTGCAATCTCAT baal35l03 TGGACTAGATTCGAAAAGGCA 19896 TGGACTAGATCCGAAAAGGCA baal35l03 GCACAAACATTATCGTTTTGT 19897 GCACAAACATCATCGTTTTGT basd12m15 CAGCCGAACTTCTAAAATATA 19898 CAGCCGAACTGCTAAAATATA

TABLE 25-8 Haruna Nijo SEQ ID Clones H602 NO: baak35p01 GTTGATCTTGGACAGCGACTC 19899 GTTGATCTTGCACAGCGACTC baak35p01 GCTAGGTACATTCGTGACATC 19900 GCTAGGTACACTCGTGACATC basd11d18 AGCTACAAAAGCAATGATGGA 19901 AGCTACAAAAACAATGATGGA BaH38E07 GTTACATCCCATCTGCTCAAA 19902 GTTACATCCCGTCTGCTCAAA bags18h13 ACAACAGATAAGTGCAATGCA 19903 ACAACAGATATGTGCAATGCA bags18h13 GTTTGTAAAACAGCAAACGTA 19904 GTTTGTAAAAAAGCAAACGTA bags18g16 GTGAAGTTGGCCCACTTGAAG 19905 GTGAAGTTGGTCCACTTGAAG bags27f21 CTGTCACCGCACCTTCAGCTG 19906 CTGTCACCGCGCCTTCAGCTG bags27f21 TTTTCGCTCGTTTCACTCTTC 19907 TTTTCGCTCGATTCACTCTTC baal39c19 TCACCCGGTGCGACGTGCCAA 19909 TCACCCGGTGTGACGTGCCAA baak46j05 ATTCTGAAGCCTACAGAAACA 19910 ATTCTGAAGCTTACAGAAACA baak46j05 GTATGCAAATCACATTAGAAA 19911 GTATGCAAATTACATTAGAAA baak46j05 TTATATTGTGGTTTAACTCAT 19912 TTATATTGTGTTTTAACTCAT baak46j05 TAACTCATGGAAATACATGGG 19913 TAACTCATGGGAATACATGGG BaGS18M02 CATCCATTGATAGGCACTGCA 19915 CATCCATTGACAGGCACTGCA bags39g08 TTCTTCGGACACATAATTTTT 19918 TTCTTCGGACGCATAATTTTT bags39g08 GCTGCATTACAGATCGCAACA 19919 GCTGCATTACGGATCGCAACA bah32g18 CTTCTCTGCCAAGCTGCGGCT 19923 CTTCTCTGCCGAGCTGCGGCT bah32g18 GGATCTGAGGCAGTGCACTTG 19924 GGATCTGAGGAAGTGCACTTG bags38l18 CATCTAAAGAAAAATGATGCT 19927 CATCTAAAGACAAATGATGCT bags38l18 TGATGATGACGGTGATGACGG 19928 TGATGATGACAGTGATGACGG bags35c13 AACAAAAGAAACATGTATTGT 19929 AACAAAAGAATCATGTATTGT bags35c13 AATGTCTTTCGAACATTGCTA 19930 AATGTCTTTCAAACATTGCTA bags35c13 GCCCACCTCAGGCATCTTGAT 19931 GCCCACCTCACGCATCTTGAT bags35c13 TGAATCAGTAAAAGAACATTC 19932 TGAATCAGTAGAAGAACATTC

TABLE 25-9 Haruna Nijo SEQ ID Clones H602 NO: bags35c13 AATATCTGGCAAATATGCTTG 19933 AATATCTGGCGAATATGCTTG bah47h04 TCCTGTGTATGTGGCGCCACT 19934 TCCTGTGTATCTGGCGCCACT bah47h04 CTTTGCAAGAGATCGGTGAAT 19935 CTTTGCAAGAAATCGGTGAAT bah47h04 GATAAAATGGAAACAGTATTA 19936 GATAAAATGGGAACAGTATTA bags30m11 AGCATTTGTGCCGATGTCTCC 19937 AGCATTTGTGGCGATGTCTCC bah22l15 CGGCCGAGCCGATCTCTTAAA 19938 CGGCCGAGCCAATCTCTTAAA bah22l15 CTGCGTTTGGTAATGGTGGGA 19939 CTGCGTTTGGCAATGGTGGGA bah22l15 GGGAGCACAATTTTGGATTCG 19940 GGGAGCACAAATTTGGATTCG bags39e15 ATGTACCTTTTTCTGGGTTAG 19941 ATGTACCTTTATCTGGGTTAG bags39e15 CTTGATGACCATTACGTTCAT 19942 CTTGATGACCGTTACGTTCAT bags39e15 GTTGGTTAAGATTATGATGAA 19943 GTTGGTTAAGGTTATGATGAA bags39e15 TTAGGAGGATTTGTTAGTGGA 19944 TTAGGAGGATCTGTTAGTGGA bags39e15 AAATCATCGACCCAGCAGTCA 19945 AAATCATCGAACCAGCAGTCA basd11h11 TCACAAACACCAAGAGAGTTG 19947 TCACAAACACAAAGAGAGTTG baak2b06 TCAATTTGATCGATCATTTCC 19948 TCAATTTGATTGATCATTTCC baak2b06 ACACTAGACCAAGGTAAAGTC 19949 ACACTAGACCCAGGTAAAGTC baak2b06 GAATGAGGACAACGCGATGGG 19950 GAATGAGGACCACGCGATGGG baak2b06 AACAAAAAGAAGCACCATCTA 19951 AACAAAAAGACGCACCATCTA baak2b06 ATCATTTCCTAAAAAAAATTG 19952 ATCATTTCCTGAAAAAAATTG baak2b06 ACTGGAAGAGAAGTACCTCTC 19953 ACTGGAAGAGGAGTACCTCTC baak2b06 TCTGGATTGAAAAAGAATGAG 19954 TCTGGATTGAGAAAGAATGAG baak2b06 ACGCGATGGGGGAGAAGGAAC 19955 ACGCGATGGGAGAGAAGGAAC basd14a23 GCGCACGGCGGCTGCCACGTC 19959 GCGCACGGCGTCTGCCACGTC basd14a23 ACAAGCATCTCATAAGCTAAT 19960 ACAAGCATCTTATAAGCTAAT basd14a23 CAAGCATCAGATCAGCTCTAC 19961 CAAGCATCAGTTCAGCTCTAC

TABLE 25-10 Haruna Nijo SEQ ID Clones H602 NO: bast25c0705 TTCCACGCTCACTAATTACTA 19962 TTCCACGCTCGCTAATTACTA bast25c0705 TGCTAAACAGTAACTTTACCG 19963 TGCTAAACAGCAACTTTACCG bast25c0705 ACCGCATATCGCCTTCTTGCA 19964 ACCGCATATCACCTTCTTGCA bast21b1204 CACTACTAAGGTAGATATAGC 19965 CACTACTAAGATAGATATAGC bast21b1204 ACCGAACAAGATGCATGCACA 19966 ACCGAACAAGGTGCATGCACA BaAK21G02 ATTCAAACTCTTCCAGCACGC 19968 ATTCAAACTCCTCCAGCACGC bastl26e1109 TCTAGAGCAAGCACTGCTGCT 19969 TCTAGAGCAACCACTGCTGCT basd13k24 CAACAGATGTGGCAGCACGTA 19970 CAACAGATGTCGCAGCACGTA baal13f02 CTAATTGGTCCGGTGTAGAGT 19971 CTAATTGGTCAGGTGTAGAGT baal13f02 ATGCAATGCATAGTCGCTTTA 19972 ATGCAATGCACAGTCGCTTTA baal13f02 CATAGCTGCCTTCAAATAAAA 19973 CATAGCTGCCGTCAAATAAAA baal13f02 CGCCAACATTTATGACACCTA 19974 CGCCAACATTGATGACACCTA bags13c10 GCATGCATGA-AGATACAGGC 19975 GCATGCATGACAGATACAGGC 19976 bags13c10 CGTATCTGCA-GCGATACATG 19977 CGTATCTGCAAGCGATACATG 19978 bags13c10 TTCACAAATC-ATCATGGCCT 19979 TTCACAAATCCATCATGGCCT 19980 bags11o11 GTCCTTTGCCGTCCTGCTCTT 19981 GTCCTTTGCCATCCTGCTCTT bags11o11 CCCAGTGTCACCGTTAAATTT 19982 CCCAGTGTCATCGTTAAATTT bags11o11 CTAGATCTTGAGTTGGTTGGT 19983 CTAGATCTTGCGTTGGTTGGT baak36p01 CCTGCTGTTCTTCTTGGCATG 19984 CCTGCTGTTCCTCTTGGCATG bah52d09 GGCTGTAATTAGGATCTGCTC 19985 GGCTGTAATTTGGATCTGCTC bah52d09 CAGACGATCTCTGAGAGTGTC 19986 CAGACGATCTTTGAGAGTGTC bah52d09 ACAGGGATTTTACAGACGATC 19987 ACAGGGATTTCACAGACGATC bah52d09 GGATCTGCTCTTTGTCTCTTG 19988 GGATCTGCTCCTTGTCTCTTG bah52d09 TCTGGGGCACTTGGACTAGCT 19989 TCTGGGGCACGTGGACTAGCT bah52d09 AGTTCAGGTGCAATTTTGGGT 19990 AGTTCAGGTGTAATTTTGGGT

TABLE 25-11 Haruna Nijo SEQ ID Clones H602 NO: BaGS26D18 TCGTCAATTGTTTTCAGACAG 19991 TCGTCAATTGCTTTCAGACAG BaGS26D18 TGCTGAAATCATGCATTCCCC 19992 TGCTGAAATCGTGCATTCCCC BaGS26D18 CAGCGCCGCGATGTTCACTCG 19993 CAGCGCCGCGGTGTTCACTCG BaGS26D18 CAGACAGAGATCGCAGAATGA 19994 CAGACAGAGACCGCAGAATGA baak33c22 ATGAATAAGCGAAGTACGACA 19995 ATGAATAAGCAAGTACGACA baak33c22 CATGGTTAAATACTCTGGTAC 19996 CATGGTTAAACACTCTGGTAC baak33c22 ATCTCAAAAGCGTCGTGTACC 19997 ATCTCAAAAGGGTCGTGTACC baak11c22 CAAGATAGTCCAAGATGCCAA 19999 CAAGATAGTCAAAGATGCCAA baak11c22 GCAGGAATCTATGGTGGCCTG 20000 GCAGGAATCTGTGGTGGCCTG bags34l06 TAAATCCAGTGGCAGAGATGG 20001 TAAATCCAGTAGCAGAGATGG bags20p21 AGCGATATATGTATGTCTTCA 20005 AGCGATATATATATGTCTTCA bah52m01 GTTCATATCATTTCCCTGTAC 20006 GTTCATATCACTTCCCTGTAC basd15d07 TATCTCTGGCGGAAGAGGTCT 20007 TATCTCTGGCAGAAGAGGTCT basd15d07 CCACTGGAGGAACATACTGAT 20008 CCACTGGAGGGACATACTGAT BaAL36B07 CAGATGCTGGGTTGTGCCAAC 20011 CAGATGCTGGATTGTGCCAAC BaAL36B07 AGACGACCCAGAAATTAACAA 20012 AGACGACCCATAAATTAACAA kr67C0206 AAGTTACTGAAAGTTAGTGTA 20015 AAGTTACTGATAGTTAGTGTA kr67C0206 GGCCACAATATGTTGGTCATT 20016 GGCCACAATACGTTGGTCATT kr67C0206 ACTTGTATCTAAAGCACTACG 20017 ACTTGTATCTTAAGCACTACG kr67C0206 GCCCGGCATAGTTAATTTTTT 20018 GCCCGGCATAATTAATTTTTT bags15j20 TGGCACCTGTAGGGAAAAGGA 20019 TGGCACCTGTTGGGAAAAGGA bags15j20 GTACAAAACAAGAATCTCCCT 20020 GTACAAAACAGGAATCTCCCT BaH15E05 CACAAGTAGTAGAAGTATTCA 20021 CACAAGTAGTGGAAGTATTCA bah58k02 TCAAATGCTCTGAGAGACTGA 20022 TCAAATGCTCCGAGAGACTGA bah58k02 TTTCACTTTCGTCTTCGCTTT 20023 TTTCACTTTCCTCTTCGCTTT

TABLE 25-12 Haruna Nijo SEQ ID Clones H602 NO: basd13d17 ACCGTTTCTAGTTGCACGGGA 20024 ACCGTTTCTATTTGCACGGGA basd13d17 ATTAGGTTCTGACCGTAGGCT 20025 ATTAGGTTCTCACCGTAGGCT basd13d17 CAGAGATTTCAAGACAATAGA 20026 CAGAGATTTCGAGACAATAGA basd13d17 TTAACAAGCGTTGTGTCACCT 20027 TTAACAAGCGCTGTGTCACCT basd13d17 TAAAAACCTAAAAAGAGACAG 20028 TAAAAACCTACAAAGAGACAG BaAK30F13 GTTCATTTGTCGAGTAGACAT 20030 GTTCATTTGTTGAGTAGACAT baal2n22 CAGCCACAGTCCTTTGGATTA 20033 CAGCCACAGTTCTTTGCATTA baal2n22 AAAGACTGAATTATTGAGTTT 20034 AAAGACTGAACTATTGAGTTT baal2n22 AACTTAAACTATTACAAAGAC 20035 AACTTAAACTTTTACAAAGAC baal2n22 CTGTAGGCAACAAAACTGTAA 20036 CTGTAGGCAAGAAAACTGTAA kr30c0705 TGGGGTTATATGGCTTAGCGG 20037 TGGGGTTATACGGCTTAGCGG BaSD19J21 AACTTAAACTTTTACAAAGAC 20038 AACTTAAACTATTACAAAGAC BaSD19J21 AAAGACTGAACTATTGAGTTT 20039 AAAGACTGAATTATTGAGTTT BaSD19J21 CATGGTTTGTACCTATATTAC 20040 CATGGTTTGTGCCTATATTAC BaSD19J21 CAGCCACAGTTCTTTGGATTA 20041 CAGCCACAGTCCTTTGGATTA BaSD19J21 CTGTAGGCAAGAAAACTGTAA 20042 CTGTAGGCAACAAAACTGTAA BaH34N22 ACCGGAACTCGCTGCCGAATC 20043 ACCGGAACTCACTGCCGAATC BaH34N22 CTTCAGCTGCTGCTGCAGCGG 20044 CTTCAGCTGCCGCTGCAGCGG BaGS1E22 GTTTATCCAGCTGATGCGGAG 20046 GTTTATCCAGTTGATGCGGAG baak34b17 CTACAAGGGAGGCAGCCCAGG 20047 CTACAAGGGAAGCAGCCCAGG baak34b17 AAAATACAGTGCCGACCAAGA 20048 AAAATACAGTCCCGACCAAGA baak34b17 GAGCTAGCGCGGGCAAGACCA 20049 GAGCTAGCGCTGGCAAGACCA kr32a0202 CAAATGCTATGGCCTATGGCT 20051 CAAATGCTATTGCCTATGGCT bags27h21 TCCGACGAGGATGAGTTCAAC 20052 TCCGACGAGGGTGAGTTCAAC bah62d17 TATAGTTACCGTACTAATACT 20053 TATAGTTACCATACTAATACT

TABLE 25-13 Haruna Nijo SEQ Clones H602 ID NO: bah62d17 TTGCTTGGAAGTCCTTCATGA 20054 TTGCTTGGAAATCCTTCATGA bah43e22 CCAAGATCTGATGTTGATGCA 20055 CCAAGATCTGGTGTTGATGCA BaAL30I23 CGGGTACGGCAGCCCGGCGAC 20056 CGGGTACGGCGGCCCGGCGAC baak34p06 TCGGAGGTCATAATGATGTTC 20059 TCGGAGGTCAGAATGATGTTC baak34p06 CGCATTGCCTACACAGCATAG 20060 CGCATTGCCTGCACAGCATAG baak34p06 AGATGGTACAAGACACAGCAG 20061 AGATGGTACAGGACACAGCAG bah63b08 CGGGTTACAACAAAAGTGGCA 20062 CGGGTTACAACAAAAGTGGCA bastl03f0812 CGGGTTACAACAAAAGTGGCA 20063 CGGGTTACAAGAAAAGTGGCA basd14m17 CAGAGCCAGAAGCAGCAGCCG 20064 CAGAGCCAGACGCAGCAGCCG basd14m17 ATCCATATCCAACAATCAGAA 20065 ATCCATATCCCACAATCAGAA basd14m17 AAAGAAGGCGCGATTGCGGAA 20066 AAAGAAGGCGAGATTGCGGAA basd14m17 TAGGCGACGGTGATGTCGAAC 20067 TAGGCGACGGGGATGTCGAAC basd2j03 TCGGCTCTGAACACCAAAGGT 20068 TCGGCTCTGACCACCAAAGGT basd2j03 AGCAGCAAACGTATAATTACC 20069 AGCAGCAAACATATAATTACC baal32b23 TGTCTCTCTCTGACTCCATCT 20070 TGTCTCTCTCCGACTCCATCT bah44n03 GCTTGTCACGAAGGTGCGGCT 20074 GCTTGTCACGGAGGTGCGGCT bah44n03 GCATCTTGGCGTCGTTGCAGA 20075 GCATCTTGGCATCGTTGCAGA bags20h05 GGGGGTTAGAGTAGAAGTAAT 20076 GGGGGTTAGAATAGAAGTAAT bags20l15 GTACAACCGTTTCCACCGTAC 20077 GTACAACCGTCTCCACCGTAC BaGS7E03 GGTGGTGGTGTGATGTCCTTC 20078 GGTGGTGGTGCGATGTCCTTC bast55e0709 TCCTTATTTGCTTCTGTCCCC 20080 TCCTTATTTGGTTCTGTCCCC baak38k04 CTTTTGTGTTTACCTCGATCT 20082 CTTTTGTGTTGACCTCGATCT baak38k04 TGCCAATCATGGGGTATTGAT 20083 TGCCAATCATAGGGTATTGAT bah42l12 TCGGCGACCGTTGCATAGGTC 20086 TCGGCGACCGCTGCATAGGTC bah42l12 GCAGGCCAGAAAAGTGTAAAC 20087 GCAGGCCAGAGAAGTGTAAAC

TABLE 25-14 Haruna Nijo SEQ Clones H602 ID NO: baet46c0905 CTGCGCGAACACGTGAAGGAA 20089 CTGCGCGAACGCGTGAAGGAA baet46c0905 GTGGGAAAGGGAACTAGTTGC 20090 GTGGGAAAGGAAACTAGTTGC bah26e10 GCTTTCTGTCACCTGATGAAT 20092 GCTTTCTGTCGCCTGATGAAT bah26e10 GAGGTTCCCGGCATTTTTCAG 20093 GAGGTTCCCGACATTTTTCAG bah32j04 GCTCTCCATGTTGAGAACTCT 20094 GCTCTCCATGCTGAGAACTCT bah32j04 AGAGAGAGCCTCTTCGATTAC 20095 AGAGAGAGCCGCTTCGATTAC bah32j04 ACATGTACAACAGATTTACAT 20096 ACATGTACAAGAGATTTACAT bah32j04 TGAAGGAGGCACAAAATGTAC 20097 TGAAGGAGGCCCAAAATGTAC bah32j04 TATGGACATGGCTACAGAATT 20098 TATGGACATGACTACAGAATT bah32j04 TTATCTCTCTCTGCTCTATTG 20099 TTATCTCTCTATGCTCTATTG bags20l07 AACCAACATATCGAAAACCTT 20100 AACCAACATAGCGAAAACCTT bags20l07 GTGTTAAGACCGTCAGCAGCT 20101 GTGTTAAGACTGTCAGCAGCT bags20l07 GAGCATGTTATACCTCTTTAT 20102 GAGCATGTTAGACCTCTTTAT bags20l07 GCTGCAGCCTGTTCTTGAAGC 20103 GCTGCAGCCTCTTCTTGAAGC bags20l07 GCTCTCCATGTTGAGAACTCT 20104 GCTCTCCATGCTGAGAACTCT bags20l07 GATCAAACCGCGAACTCAATG 20105 GATCAAACCGTGAACTCAATG bags20l07 CTCAATGACTGGAGCATGTTA 20106 CTCAATGACTTGAGCATGTTA bags20l07 AGGAGGCCCATTGCAGAAGAT 20107 AGGAGGCCCAATGCAGAAGAT kr18c0505 ATTTGGCAGAGACGAAATGCG 20108 ATTTGGCAGACACGAAATGCG kr18c0505 TGGACCATACGTGATATCCAT 20109 TGGACCATACTTGATATCCAT kr18c0505 GCTTTTGGGGGGGTCATCTAG 20110 GCTTTTGGGGCGGTCATCTAG kr18c0505 ACAAAAATCATCAGATAATGT 20111 ACAAAAATCAGCAGATAATGT kr18c0505 AAACACTATCGTTACGCCTAT 20112 AAACACTATCATTACGCCTAT kr18c0505 TGATGATTGATCAGGGCCTGA 20113 TGATGATTGAACAGGGCCTGA basd11m24 TGCAGAAACTGTGTTGCTAAA 20114 TGCAGAAACTATGTTGCTAAA

TABLE 25-15 Haruna Nijo SEQ Clones H602 ID NO: basd1i15 TTGTCTAGTGTTTGGGCTTGA 20115 TTGTCTAGTGCTTGGGCTTGA basd1i15 ACAAATCTACTTTCGCGAAGG 20116 ACAAATCTACATTCGCGAAGG bah13i21 TTGCAGGAAATACTGCAGAAC 20119 TTGCAGGAAACACTGCAGAAC basd27o20 CCTGTATGAACTTCGAAATGA 20120 CCTGTATGAATTTCGAAATGA baak45l08 TTATTCACTACCAACTTTGTA 20123 TTATTCACTAGCAACTTTGTA baak45l08 GGATGCCCGTTCCATGATCAA 20124 GGATGCCCGTCCCATGATCAA baak45l08 AGAATATCCAAGGACAAAGGT 20125 AGAATATCCATGGACAAAGGT baak45l08 TTGGCACAAGGGCAGCAGTAA 20126 TTGGCACAAGAGCAGCAGTAA baak45l08 AGGGCATTCAGTTTCGTTCCA 20127 AGGGCATTCAATTTCGTTCCA baak45l08 CTGCCAGCCCCCCGACGGTGA 20128 CTGCCAGCCCTCCGACGGTGA baak45l08 GCCAGCTCTCCACGCCGGACC 20129 GCCAGCTCTCAACGCCGGACC baak45l08 CCTCCCACAATTAAGCAGTGC 20130 CCTCCCACAAATAAGCAGTGC BaAL36N04 GCCAGAGAAATTTTTCCTCGA 20131 GCCAGAGAAAATTTTCCTCGA baak41d17 ACATGCATGGTCAATTTTACG 20132 ACATGCATGGACAATTTTACG baak41d17 GTGGACTTCTCCTTGGCGGCG 20133 GTGGACTTCTTCTTGGCGGCG baak41d17 TGGCCTCGCCGCCCATCTTTT 20134 TGGCCTCGCCACCCATCTTTT baak41d17 TCGGTGGCCGTCTTCTTCACG 20135 TCGGTGGCCGGCTTCTTCACG baak41d17 TTCACGGCCTCCGTCTTCTCG 20136 TTCACGGCCTACGTCTTCTCG baak41dl7 TCTGCAGCGGTGTCCTGGACA 20137 TCTGCAGCGGCGTCCTGGACA BaGS25M06 CTAAACCAGGGCGCCTGAACA 20139 CTAAACCAGGACGCCTGAACA BaGS25M06 CGATGTAGACGTGGACGTTGA 20140 CGATGTAGACATGGACGTTGA bah36f21 ATGTCTCCGCTGGCGGTGCGT 20141 ATGTCTCCGCCGGCGGTGCGT bah36f21 CTTGCCATCCACGTCGCCGTC 20142 CTTGCCATCCCCGTCGCCGTC baak13b12 ATGCGGTCCAAACTCCAACCA 20143 ATGCGGTCCACACTCCAACCA baak13b12 TGTTCCTTCCGCTTGTTTGTT 20144 TGTTCCTTCCTCTTGTTTGTT

TABLE 25-16 Haruna Nijo SEQ Clones H602 ID NO: bast63B0604 GAGGAGCCAGCAGCAGCATCT 20145 GAGGAGCCAGAAGCAGCATCT baak11n06 AAACATGATCTTGATCCATGC 20146 AAACATGATCCTGATCCATGC kr13f1012 GTGTCTCGCTCGGTTCGTTCT 20147 GTGTCTCGCTGGGTTCGTTCT kr13f1012 TCCCTTCTTCCTTTTTTTTGT 20148 TCCCTTCTTCGTTTTTTTTGT bags20k06 GTCCTGGGATCTGAAGGAATC 20149 GTCCTGGGATTTGAAGGAATC bags20k06 CTTCCGTCGAGCAACCCAAAC 20150 CTTCCGTCGAACAACCCAAAC bags20k06 CAGCTTCATCAAAAGAATCGA 20151 CAGCTTCATCGAAAGAATCGA baak15p20 CTGATGAACATCTAAGATTTC 20152 CTGATGAACACCTAAGATTTC BaH23 J08 ACGGATTCTAGGCATGATCAA 20153 ACGGATTCTAAGCATGATCAA BaH23 J08 ATAGCTTACCACAATGGTCTC 20154 ATAGCTTACCGCAATGGTCTC baet30b1004 TGGACGACTAGGAATTATTGG 20155 TGGACGACTAAGAATTATTGG bastl33h0816 CCGATTAACAGTGGCCCACAC 20156 CCGATTAACACTGGCCCACAC baak42k19 CTTTGGCGTTCCCTTTTCCGC 20162 CTTTGGCGTTGCCTTTTCCGC bast60a1101 GAACTTGCCTCCTGTCCTTTA 20163 GAACTTGCCTACTGTCCTTTA bast60a1101 TATGGCACCCATCACACCACA 20164 TATGGCACCCCTCACACCACA bah52l06 TCGGGCCCCACTCCCTCATGT 20165 TCGGGCCCCAATCCCTCATGT bah52l06 CTAAGTACCGTGTCCACCTCT 20166 CTAAGTACGGCGTCCACCTCT bah23h10 GAAGTGTAGCGTCAGGGTTGT 20167 GAAGTGTAGCATCAGGGTTGT bah23h10 GCAAAGAATAAGGGCCGCCTT 20168 GCAAAGAATACGGGCCGCCTT bagsl5o23 GATTTTAATTCAGATGATGAT 20169 GATTTTAATTAAGATGATGAT bagsl5o23 CCTTATCATCTTCCTGTACCA 20170 CCTTATCATCATCCTGTACCA bagsl5o23 TGATGAACCACTTGTTGATTT 20171 TGATGAACCAGTTGTTGATTT bagsl5o23 AGAGGTTGCACTCTTAACCGA 20172 AGAGGTTGCAGTCTTAACCGA baak20e08 CTATCCAAGAAGTTGAACATC 20173 CTATCCAAGAGGTTGAACATC baak20e08 CGCGGAGGCCAATGGTGTAGT 20174 CGCGGAGGCCGATGGTGTAGT

TABLE 25-17 Haruna Nijo SEQ Clones H602 ID NO: baak20e08 CCACCTCGTAGGGGATCTCCG 20175 CCACCTCGTATGGGATCTCCG baak20e08 AATCAACCCAAGGTGAAATTG 20176 AATCAACCCAGGGTGAAATTG kr61a1202 ATCTGAAATTGTGAGCATGAA 20177 ATCTGAAATTATGAGCATGAA kr61a1202 GTGTCCAAAACCGTGATGTTC 20178 GTGTCCAAAAACGTGATGTTC bast03f0212 CGGACGATATCAGCTAGCTAG 20179 CGGACGATATGAGCTAGCTAG baak30o17 TTAATCCTTCGTAGGCTCATT 20181 TTAATCCTTCATAGGCTCATT baak30o17 TTTTTCCTCCAATCCAGATGC 20182 TTTTTCCTCCCATCCAGATGC baal2n04 AGAGGACACCGCCGATAGCAA 20186 AGAGGACACCACCGATAGCAA bags30n15 CACCATCTTGCAGTAAATACA 20188 CACCATCTTGAAGTAAATACA baal34d18 TGTAGAAGGTTCTCCTGTCGT 20189 TGTAGAAGGTGCTCCTGTCGT bags37i06 TTCCCTGATGCAGTTCTTCAA 20191 TTCCCTGATGTAGTTCTTCAA BaGS19N09 ACTGGTAGACGAGGTCCATGC 20192 ACTGGTAGACAAGGTCCATGC BaGS19N09 ACCCGTCCACCAGCAGGGAGT 20193 ACCCGTCCACGAGCAGGGAGT BaSD13H09 CTCCGGATCCGCCGCGACGAA 20194 CTCCGGATCCACCGCGACGAA BaSD13H09 AACTCAAGCTCGCCTCTAACA 20195 AACTCAAGCTTGCCTCTAACA baak28o08 CAGGATAACTATCTGAACCCT 20197 CAGGATAAGTGTCTGAACCCT BaH15K08 CCGAGATTCGATGCCCAGGTA 20198 CCGAGATTCGGTGCCCAGGTA bah28a11 TCATACAAAACAAGAAAAAAC 20201 TCATACAAAAGAAGAAAAAAC bah28a11 ACACTTTTTCATCCTTGACTG 20202 ACACTTTTTCGTCCTTGACTG basd25e01 AGGTATTAAGATTAACTGCTG 20203 AGGTATTAAGCTTAACTGCTG baet23F0111 AACTCAATCAGGCCTTGTGCT 20204 AACTCAATCAAGCCTTGTGCT baet23F0111 AAATTGGATTCATGTACTAAC 20205 AAATTGGATTTATGTACTAAC baet23F0111 AATGCACCCTTTGTCCTGCAA 20206 AATGCACCCTGTGTCCTGCAA basd11c04 TCTAACGAGATCACAACAAGA 20208 TCTAACGAGAGCACAACAAGA basd11c04 TGTCGTTGCCAGCCACTTCTC 20209 TGTCGTTGCCGGCCACTTCTC

TABLE 25-18 Haruna Nijo SEQ Clones H602 ID NO: bags22Kl7 AAACACAGCTACTAAAATAAG 20210 AAACACAGCTGCTAAAATAAG bags22kl7 TCTAATAATTGATTCGCTTCA 20211 TCTAATAATTAATTCGCTTCA bags22a16 AAAATATGCTCAAGAGTTCAA 20212 AAAATATGCTTAAGAGTTCAA bags22a16 CAAAAGAGCAACAAAAATTCA 20213 CAAAAGAGCAGCAAAAATTCA bags22a16 GAACATGATA-GAGGTATGTT 20214 GAACATGATAAGAGGTATGTT 20215 baak41l23 ACCTAACTAATTCACACCAGC 20219 ACCTAACTAAATCACACCAGC baak41l23 GTAATCAACTGATTCCTTTGG 20220 GTAATCAACTAATTCCTTTGG bastl26c1206 ATGATGCCTTAATTGAGCTCA 20223 ATGATGCCTATATTGAGCTCA bah27p20 TGGTTTCTGACGCTTTCAGAA 20224 TGGTTTCTGATGCTTTCAGAA bah27p20 CGATCATAACGTGGACGGCGG 20225 CGATCATAACATGGACGGCGG baal12d24 TTCAAGTCCTTTCTCTTATAC 20226 TTCAAGTCCTCTCTCTTATAC bastl41f0511 TCGACCGTGGAAATCCCAACA 20227 TCGACCGTGGGAATCCCAACA bastl41f0511 CTAGGAGTAAGTCCCCACGTC 20228 CTAGGAGTAACTCCCCACGTC

TABLE 26-1 Haruna Nijo SEQ Clones H602 ID NO: baal10j04 AGGCAAACTCAGTCAGGGAAA 20230 AGGCAAACTCGGTCAGGGAAA BaAK33J17 CAACCCAACTTGATGATAATG 20231 CAACCCAACTCGATGATAATG bast17h0515 CCTACAAGTACTACAACTCAC 20233 CCTACAAGTAGTACAACTCAC baak35a06 TCACATGCTTTCGGTACGATT 20234 TCACATGCTTCCGGTACGATT baak35a06 ATACACAGTCCCCTGTAATAT 20235 ATACACAGTCACCTGTAATAT baak18m22 GGAAGTGTGCTTGGGCATTCG 20239 GGAAGTGTGCCTGGGCATTCG baak18m22 AAATCATGCACAAGAGGCGAT 20240 AAATCATGCAGAAGAGGCGAT baak18m22 CCCCATGCTCATGCAGGCATG 20241 CCCCATGCTCTTGCAGGCATG baak18m22 CGGAAGATGCAATGCCGACGC 20242 CGGAAGATGCGATGCCGACGC bast26g0614 TCACCAAGTTACCGTCTTCAG 20243 TCACCAAGTTGCCGTCTTCAG basd22f13 AATGCGGTATCGTCCGACCTC 20244 AATGCGGTATTGTCCGACCTC basd22f13 GTAGTGCAGAGGACAGTGATG 20245 GTAGTGCAGATGACAGTGATG bah63l18 ATTCTGTTTGACAGGATCTCA 20249 ATTCTGTTTGTCAGGATCTCA bah63l18 TCTCGGCCTCATCAAAGAAGA 20250 TCTCGGCCTCGTCAAAGAAGA BaGS9H22 TCAAGTCTGGTACCACTCTAT 20251 TCAAGTCTGGAACCACTCTAT BaGS9H22 ATGCAGCACAGACAACACGTT 20252 ATGCAGCACAAACAACACGTT BaGS9H22 GTACAACTCACGAGCTCTTGT 20253 GTACAACTCAAGAGCTCTTGT BaGS9H22 GAGCTCTTGTAGACGGTTTTC 20254 GAGCTCTTGTCGACGGTTTTC BaGS9H22 CGTTCCAAATGCGTGGTCCCA 20255 CGTTCCAAATACGTGGTCCCA BaGS9H22 ATATCTGGCACTGCAAGGTGG 20256 ATATCTGGCATTGCAAGGTGG BaH17N17 CCGGCACAAGTTCACATATCC 20259 CCGGCACAAGCTCACATATCC BaH17N17 CCGGATTTTCACGTCCGATTC 20260 CCGGATTTTCGCGTCCGATTC bags34a05 ACACGAGTCCTTTACAGAATA 20261 ACACGAGTCCCTTACAGAATA bags34a05 TCATACCAGTGATGCTTTTAT 20262 TCATACCAGTCATGCTTTTAT bags1m23 AAAAAGAGACGACGTGCACTT 20263 AAAAAGAGACAACGTGCACTT

TABLE 26-2 Haruna Nijo SEQ Clones H602 ID NO: bags1m23 TTTTGCCTTGACTATCTGGCT 20264 TTTTGCCTTGGCTATCTGGCT BaH50B05 ACATTTCAGAATTGTGTCACT 20265 ACATTTCAGAGTTGTGTCACT BaH50B05 TGACTTCCTATCTTGTGAAAT 20266 TGACTTCCTACCTTGTGAAAT BaH50B05 CCAAGTTCTCCTACTCTTCTC 20267 CCAAGTTCTCTTACTCTTCTC BaH50B05 AGGTGCAGTTTTACCTAACAT 20268 AGGTGCAGTTCTACCTAACAT BaH50B05 TATTCGCTGCACTCGTTGGTC 20269 TATTCGCTGCCCTCGTTGGTC bags1h11 TGGCGGCCTCAACCATGTTGG 20270 TGGCGGCCTCGACCATGTTGG bags1h11 GACCGCAGTCAGGGTTCACCC 20271 GACCGCAGTCGGGGTTCACCC bah47e01 TGCAGGGGTGCTTCACAGTGC 20274 TGCAGGGGTGGTTCACAGTGC bast52E0109 TGACGCTTGATAGCTCATCAT 20275 TGACGCTTGACAGCTCATCAT bags1o08 AGGACCCATGGGTGATGCATT 20276 AGGACCCATGTGTGATGCATT bags1o08 TGAAGACTAGACTGACAGCAG 20277 TGAAGACTAGGCTGACAGCAG baak32l14 CTAGCACTCAAGTTCCGAGGA 20279 CTAGCACTCAGGTTCCGAGGA baak32l14 CCTGCTACCGTGAAAGGGCCG 20280 CCTGCTACCGCGAAAGGGCCG bah50o06 CGCCCAGCCGATATGCAATCT 20281 CGCCCAGCCGTTATGCAATCT bah50o06 ATATGATCCACTTTTCATGAT 20282 ATATGATCCATTTTTCATGAT bah50o06 TCATGTATACCGGGAAGGAAG 20283 TCATGTATACAGGGAAGGAAG bah50o06 ACATGCATACATGTTATAAAT 20284 ACATGCATACTTGTTATAAAT bah50o06 GTATGTCGCAATTGATCTAAA 20285 GTATGTCGCATTTGATCTAAA bah50o06 CTCGTTATATTTGTACAGGTC 20286 CTCGTTATATCTGTACAGGTC BaSD26I01 TATTCCAGTAAAGTGATATTA 20287 TATTCCAGTACAGTGATATTA BaSD26I01 AGATTGCAGACGATTTCAAAT 20288 AGATTGCAGATGATTTCAAAT BaSD26I01 AATGTGACTACCATGTCAGAC 20289 AATGTGACTAACATGTCAGAC bags1h24 TGCTCAGCCAGCGCCAAGATC 20290 TGCTCAGCCAACGCCAAGATC basd27g16 GGCTGCTCCTGGTCTTAAGAA 20291 GGCTGCTCCTAGTCTTAAGAA

TABLE 26-3 Haruna Nijo SEQ Clones H602 ID NO: basd27g16 AAATGAAATAATTGGCTGCTC 20292 AAATGAAATAGTTGGCTGCTC bags34c19 TCGTAAGTGGCCGCAGAACGT 20294 TCGTAAGTGGTCGCAGAACGT bags34c19 ACAAGTGTTCGTGTTCTGGCT 20295 ACAAGTGTTCATGTTCTGGCT bags34c19 TACAGTGTGAAGCCTGCAGTC 20296 TACAGTGTGAGGCCTGCAGTC bah20k17 ACAAATTCAAGGTCTCCAGAA 20297 ACAAATTCAACGTCTCCAGAA baak29c12 TGAGATGAAATAACACACTTT 20299 TGAGATGAAAGAACACACTTT baak29c12 CTTTGAAGGAGGTTAGTTAAA 20300 CTTTGAAGGAAGTTAGTTAAA baak29c12 GCGATCTGCAGGAGCACCCTG 20301 GCGATCTGCAAGAGCACCCTG baak29c12 ACTGACCTGCCACGGCTCCAA 20302 ACTGACCTGCGACGGCTCCAA baal6a09 GAATAAGAAAACATTTCTTCC 20304 GAATAAGAAAGCATTTCTTCC bah30p15 GCCCTTCATCGGACCACACTT 20306 GCCCTTCATCTGACCACACTT basd25d22 CACAGCAGGGGTCCGGCCAAG 20308 CACAGCAGGGTTCCGGCCAAG baak13n10 AGAAAGGTGCGTACATAATTG 20310 AGAAAGGTGCATACATAATTG bags18j21 CCTTGTGACCGTCATACATCG 20311 CCTTGTGACCTTCATACATCG bags18j21 AGGTCACAATAGGTTGATCAA 20312 AGGTCACAATGGGTTGATCAA bags33j02 GATAAGATACATGACGACTGT 20313 GATAAGATACGTGACGACTGT bags33j02 AAAACAAACGTTCGGATGAAT 20314 AAAACAAACGGTCGGATGAAT bags37f05 GAGATGTCATCGACCGTGCAT 20316 GAGATGTCATTGACCGTGCAT bags37f05 AACACCCCCGCGCCTTTTCGA 20317 AACACCCCCGTGCCTTTTCGA bags37f05 ATTGCGATGCGTAACGGGATA 20318 ATTGCGATGCATAACGGGATA BaAL17L15 CAGTCCGAGCCGCCCCCGCCG 20319 CAGTCCGAGCTGCCCCCGCCG bags20h12 TGCTAGAATCATGTATATGGC 20320 TGCTAGAATCCTGTATATGGC bags20h12 CACTGTAAATGTTCAGCCTAT 20321 CACTGTAAATTTTCAGCCTAT bags20h12 ATCTGAAAAAAGGTGATGATT 20322 ATCTGAAAAAGGGTGATGATT kr65a0802 TGTTCTTGTACTCGGCAATGG 20323 TGTTCTTGTAATCGGCAATGG

TABLE 26-4 Haruna Nijo SEQ Clones H602 ID NO: kr65a0802 CGTCGTGGTCCTTCTACCGCG 20324 CGTCGTGGTCGTTCTACCGCG basd22j16 CTATAAGCATCGTCAAGTGAA 20325 CTATAAGCATTGTCAAGTGAA bah39n18 GCAGACACTGTGGCACCAGAC 20326 GCAGACACTGGGGCACCAGAC bah39n18 GAACGTGTCGGGAAACCTCGA 20327 GAACGTGTCGTGAAACCTCGA bags20d19 CTATAATGTGAAGGTACATCC 20328 CTATAATGTGGAGGTACATCC bags20d19 GCATGGTATCGCCCGTATGCC 20329 GCATGGTATCACCCGTATGCC bags20d19 ACTTGCGGAGAACTGCCGATC 20330 ACTTGCGGAGGACTGCCGATC bags20d19 TCGATTTTTAGATTGCTATAA 20331 TCGATTTTTACATTGCTATAA bags20d19 GATAGGTGAAGCACTGCATGG 20332 GATAGGTGAAACACTGCATGG bags20d19 TCACTGCTCTCCAGATGAAAC 20333 TCACTGCTCTGCAGATGAAAC bags20d19 GAAACTGCCAACACAAAAGAT 20334 GAAACTGCCAGCACAAAAGAT bags20d19 CAAAAGATTGTTTGCTTATTT 20335 CAAAAGATTGATTGCTTATTT bags34h19 AAGTCCTAACCAACCAGCAAT 20336 AAGTCCTAACAAACCAGCAAT bags34h19 CATACCAAATCAAATGTTACA 20337 CATACCAAATTAAATGTTACA bags34h19 TTCATATCCACGACCCCATTA 20338 TTCATATCCATGACCCCATTA baak42n10 ATTACCAGTAAATAAATTTCT 20339 ATTACCAGTAGATAAATTTCT baak42n10 GAAAGCACTTTCCATCTAACT 20340 CAAAGCACTTCCCATCTAACT basd24f03 TCACCATGGGGTTTGATTCCA 20341 TCACCATGGGATTTGATTCCA BaGS23N21 GAAATATATATAGAACAGAGG 20342 GAAATATATAAAGAACAGAGG BaGS23N21 TCCAAACAGTCCGATGCCAAA 20343 TCCAAACAGTTCGATGCCAAA BaGS23N21 CTTCAGAGCATCAATGCCTCT 20344 CTTCAGAGCAGCAATGCCTCT BaGS23N21 GCTCCCAACAATTCTAAATTC 20345 GCTCCCAACAGTTCTAAATTC BaGS23N21 TATAAGTCTTTTAAGAGATTC 20346 TATAAGTCTTCTAAGAGATTC B9GS23N21 TCTCTTAGTAGAATCTCTAGA 20347 TCTCTTAGTAAAATCTCTAGA BaAK45E04 CATTCAACGAGAGCGAAGCCA 20348 CATTCAACGAAAGCGAAGCCA

TABLE 26-5 Haruna Nijo SEQ Clones H602 ID NO: bags5n23 CCTTGGGCACCGACGCCGTCA 20349 CCTTGGGCACAGACGCCGTCA bast52h1216 CAACAGTTGTGACAAAAGAGA 20350 CAACAGTTGTAACAAAAGAGA bags22f23 TGTGTCCTTCACAAAGGAGAT 20351 TGTGTCCTTCCCAAAGGAGAT bags22f23 TCCTGTGGTTTCATACAGGCA 20352 TCCTGTGGTTCCATACAGGCA bags22f23 TATGCTAGTTCTCTCGATTGC 20353 TATGCTAGTTTTCTCGATTGC BaH38E02 ACAGAGCCGGCGGACAGAAAC 20354 ACAGAGCCGGGGGACAGAAAC BaH38E02 GATGTTCTTGTTACTCCGACG 20355 GATGTTCTTGCTACTCCGACG BaH50O07 TTCTTAAGTTTATATACATCT 20356 TTCTTAAGTTAATATACATCT BaH50O07 AAGAGGTGTCTGTTGGCAATG 20357 AAGAGGTGTCCGTTGGCAATG BaH50O07 CGAGATTGAATGAAAGTAATG 20358 CGAGATTGAACGAAAGTAATG BaH50O07 GATCAGTCTTTATTTCTATTG 20359 GATCAGTCTTCATTTCTATTG kr07c1006 GTTCATCCTTGCTTACATTAC 20360 GTTCATCCTTACTTACATTAC baak26a02 GGCAAGTTATCATAAATAATA 20361 GGCAAGTTATGATAAATAATA baak2j22 CGATGGAAACAAGCTCGCCAT 20362 CGATGGAAACCAGCTCGCCAT baak2j22 AGAACAAGGAGGCTGAGCGCT 20363 AGAACAAGGAAGCTGAGCGCT bah45h23 AAGGTTTACGTTATGCTATGC 20364 AAGGTTTACGGTATGCTATGC bah45h23 ATTGACCTTGTTCTTCTTCAG 20365 ATTGACCTTGGTCTTCTTCAG bah45h23 CTGTGCAGATAATGACTTGGC 20366 CTGTGCAGATTATGACTTGGC baak46m16 AACTATGCACACAACATATAA 20367 AACTATGCACCCAACATATAA baak46m16 TTTATTCTATAAGGCTAGATG 20368 TTTATTCTATCAGGCTAGATG baak46m16 AACAGCAGCACAACTAATCAC 20369 AACAGCAGCAAAACTAATCAC bah55m23 TCTTCTTCTTTGCCTCTCCTC 20370 TCTTCTTCTTCGCCTCTCCTC bah59c05 ACAGGTCGCTTGCGGCCCCGT 20371 ACAGGTCGCTCGCGGCCCCGA basd11l17 TGAACTTGGCATAGATGTCAG 20372 TGAACTTGGCGTAGATGTCAG bahl9e08 GTCGCCGGAGTGGGAAATGCA 20373 GTCGCCGGAGAGGGAAATGCA

TABLE 26-6 Haruna Nijo SEQ Clones H602 ID NO: bahl9e08 CGCTGGAGCCAAAAACGGGCT 20374 CGCTGGAGCCGAAAACGGGCT BaAK21N24 CACATAGTGAACTGAACTCTC 20375 CACATAGTGAGCTGAACTCTC BaAK21N24 AGGTGGTGGTGTCCTAAATTC 20376 AGGTGGTGGTATCCTAAATTC BaAK21N24 GAAATAGAGCATACATGCAGA 20377 GAAATAGAGCTTACATGCAGA bah50e09 AATTATTTATCTACGAGAGAT 20378 AATTATTTATTTACGAGAGAT baak17p18 TCTCCATTTCCCATCACAACC 20379 TCTCCATTTCTCATCACAACC baak17p18 AAGCAATCATTACCATGATAA 20380 AAGCAATCATGACCATGATAA baal7a04 TCTGTCACGCAGCCAAGGCAC 20381 TCTGTCACGCGGCCAAGGCAC bast47b0303 GATGACCATTCCAAACTTCTC 20382 GATGACCATTGCAAACTTCTC bah28o17 TCTGAAGCTCGATCTTCCCCG 20383 TCTGAAGCTCAATCTTCCCCG bah37k03 ATCTCTCTCAGTTTTCCTCCA 20384 ATCTCTCTCAATTTTCCTCCA bah37k03 TGTTTGCCGGTTCAGCTTTGT 20385 TGTTTGCCGGGTCAGCTTTGT bastl38A0501 CAGCCAAAGCACAGCTCACCA 20386 CAGCCAAAGCGCAGCTCACCA bags23c03 GTATACAACTATAAGCACGCA 20387 GTATACAACTTTAAGCACGCA basd13j01 GTTTTCTGACGGTAGTTGATG 20388 GTTTTCTGACAGTAGTTGATG basd13j01 CATGGTTATCTCTGTAGTCGC 20389 CATGGTTATCCCTGTAGTCGC bags21c02 GTATTGAGCAGAGAGAGGTGG 20390 GTATTGAGCACAGAGAGGTGG bags21c02 GCATCATGGCAACCATAGAAA 20391 GCATCATGGCGACCATAGAAA bags6k10 ATATAGATGTGTATGCATGTA 20394 ATATAGATGTATATGCATGTA bags9k18 TCATATGGCAGCTCTGTGAGG 20395 TCATATGGCAACTCTGTGAGG bags9k18 TTTCCTCATCACACACGACGT 20396 TTTCCTCATCGCACACGACGT bags9k18 TGCATACCGAAGGTGGAAGCT 20397 TGCATAGCGACGGTGGAAGCT baal18c19 AAAAGGGTAC-GTTTGATGTA 20398 AAAAGGGTACCGTTTGATGTA 20399 baal18c19 AGGATCTTGGNTATAACCTCT 20400 AGGATCTTGGTTATAACCTCT baal18c19 GTGCAATGCA-TTTCTACAAA 20401 GTGCAATGCAATTTCTACAAA 20402

TABLE 26-7 Haruna Nijo SEQ Clones H602 ID NO: baak27p14 AATATATATATGTTATTAGTC 20403 AATATATATACGTTATTAGTC baak27p14 AGCATATATTCGAAAGATGAC 20404 AGCATATATTTGAAAGATGAC baak27p14 CTTGCTTAGGNGGCATCTATG 20405 CTTGCTTAGGTGGCATCTATG baak27p14 GATAGTCTAAAAGATGCATGA 20406 GATAGTCTAANAGATGCATGA bags30n21 ACTGGAAATCGACAATGATAT 20407 ACTGGAAATCCACAATGATAT bah55p23 TGTTTACATCATAGATTCAGT 20408 TGTTTACATCGTAGATTCAGT bah55p23 AGGCTAGCGCAGCTGGAGGCA 20409 AGGCTAGCGCGGCTGGAGGCA bah54p22 ACCCAGAACCGAACCAAAACT 20410 ACCCAGAACCAAACCAAAACT bah54p22 GTCCAGTACCGTACTCTTCAA 20411 GTCCAGTACCATACTCTTCAA bags34f05 AGTGATGCCTCCTGAGACGCA 20412 AGTGATGCCTACTGAGACGCA bags34f05 CGCCTACGTCAGGCAGGCCTA 20413 CGCCTACGTCGGGCAGGCCTA bah14a24 ACGAAATGAGCCGCCACAGAA 20416 ACGAAATGAGACGCCACAGAA bah50j11 ATATTTTCCACTATGTGTTAT 20417 ATATTTTCCAATATGTGTTAT basd22l21 GAGGCGGGATTTTGATGTCAA 20424 GAGGCGGGATGTTGATGTCAA basd22l21 TATTTCCAAGCGTCCTTAGTT 20425 TATTTCCAAGTGTCCTTAGTT kr27e0909 TAACTTGTGAGTCGAGCCATT 20426 TAACTAGTGACTCGAGCCATT bastl41H0216 TGGCTTTATCAACTTTCTCGC 20427 TGGCTTTATCGACTTTCTCGC baak36o17 TGCTCTTGCAGAAGTGTGGGG 20428 TGCTCTTGCANAAGTGTGGGG BaH31P15 AAACAGAATTAAAGGACACAC 20429 AAACAGAATTGAAGGACACAC bags9h03 ACAAAGTCCGTCGCAGAGCTT 20431 ACAAAGTCCGCCGCAGAGCTT basd26d07 TCAGTGAGCTTTTTATGCCTT 20442 TCAGTGAGCTGTTTATGCCTT bah21h17 TAACTGGGATCCGTTTAGTTT 20444 TAACTGGGATTCGTTTAGTTT bags5d21 GCTGATGAAAAATAGACAGTA 20445 GCTGATGAAAGATAGACAGTA baak34d14 GATTCGTTCTGCTGAACTCGT 20446 GATTCGTTCTCCTCAACTCGT baak18a16 GTCTGCACACAACTTACAAAG 20447 GTCTGCACACGACTTACAAAG

TABLE 26-8 Haruna Nijo SEQ Clones H602 ID NO: baak18a16 TTCACCTTGAGTTCTTCGAAC 20448 TTCACCTTGAATTCTTCGAAC bags4b01 CATTTCTACGCCGCCTTGTTA 20449 CATTTCTACGTCGCCTTGTTA bags4b01 TGTTATGTGGCGAAATAGATG 20450 TGTTATGTGGTGAAATAGATG bags38c19 AGTTAAATTTCTGTGCACAGG 20451 AGTTAAATTTTTGTGCACAGG bags38c19 CGCTGTGGGATCAGTTAAATT 20452 CGCTGTGGGAGCAGTTAAATT bags38c19 GCCAAGAGTGACACTCGACAA 20453 GCCAAGAGTGGCACTCGACAA bags38c19 GCTATATTGGTGCTGACTATC 20454 GCTATATTGGGGCTGACTATC kr42d0208 CAAAGTAAGCGGCTGAATATT 20456 CAAAGTAAGCCGCTGAATATT BaAK35N11 CCCATGACCGATACGACTGGG 20460 CCCATGACCGGTACGACTGGG bags26n10 CCACCTATGAGTCGAATCTTC 20461 CCACCTATGAATCGAATCTTC bags37n02 GCCATAACCTTTATTATGGAC 20462 GCCATAACCTCTATTATGGAC bags37n02 CAGAAGAAGCGGTGGTTTCTC 20463 CAGAAGAAGCAGTGGTTTCTC bags37n02 CTATAGCATATTAGCCAGACC 20464 CTATAGCATAGTAGCCAGACC baak26l11 TATACCTGTGCTAGATGTGTG 20465 TATACCTGTGGTAGATGTGTG BaH57C19 CAACTACCTTCCTGTCAAGCC 20466 CAACTACCTTTCTGTCAAGCC bags7a01 GGCGCCTCCCGCTGCACCTCG 20467 GGCGCCTCCCACTGCACCTCG bah19l15 CACATGAAGCTGTGGGCACGT 20468 CACATGAAGCCGTGGGCACGT baal4o02 TATTCTTTCAACCTTACATCT 20469 TATTCTTTCAGCCTTACATCT baal4o02 AGCACCGCACACCAATGTTAC 20470 AGCACCGCACGCCAATGTTAC baal4o02 CAAGGTAGCTCTCAGTGCTGG 20471 CAAGGTAGCTTTCAGTGCTGG baal4o02 ATTAAGCTGATAGGTCTGACT 20472 ATTAAGCTGAAAGGTCTGACT bah58a12 TGGTAGAGACGAATCACATCG 20473 TGGTAGAGACAAATCACATCG bah58a12 TGGTAAATTCGTCAAGATTCA 20474 TGGTAAATTCATCAAGATTCA bah58a12 TCTGAGAAAGTCCGTCATCTT 20475 TCTGAGAAAGCCCGTCATCTT bah58a12 AAGTGTCGAAGCTGTCTTCCC 20476 AAGTGTCGAATCTGTCTTCCC

TABLE 26-9 Haruna Nijo SEQ Clones H602 ID NO: bahl1b08 CCGTTTGTACCTGTGGTGCAA 20477 CCGTTTGTACTTGTGGTGCAA bahl1b08 TCTTGTATAAGCAATGGGCTA 20478 TCTTGTATAAACAATGGGCTA bahl1b08 GTCTCGCCATTATGTGCAGTT 20479 GTCTCGCCATGATGTGCAGTT bahl1b08 ATCCTCATGATTTCTTCATAG 20480 ATCCTCATGAATTCTTCATAG bags14m15 ACTGCATGCAAGTACATCATG 20481 ACTGCATGCATGTACATCATG bags14m15 CGACGTTACGTTGTAGAACTG 20482 CGACGTTACGGTGTAGAACTG bags14m15 CAACCTCTGAAATGGGTCCTG 20483 CAACCTCTGACATGGGTCCTG bags14m15 TTCAAACCGGGAACCTTGAAA 20484 TTCAAACCGGAAACCTTGAAA bastl04b1103 GCGTTCCCAGTCACGAGTTTC 20486 GCGTTCCCAGCCACGAGTTTC bags13g08 CCAAATGCTTGATATGAGCTT 20487 CCAAATGCTTCATATGAGCTT bags13g08 ATGCCGATCCAAAAGGGACAC 20488 ATGCCGATCCGAAAGGGACAC bags13g08 AAAGGGACACCAAGCAGGCAA 20489 AAAGGGACACGAAGCAGGCAA basd27p03 GTGGAGGAACTTACAACCTGG 20490 GTGGAGGAACCTACAACCTGG basd27p03 AGAAATACCCATTTCTGGCAA 20491 AGAAATACCCGTTTCTGGCAA basd27p03 CTGCACGCTAGGTGGAGGAAC 20492 CTGCACGCTAAGTGGAGGAAC bast38D0707 TTTAGGGGATCGGTTAGAGTT 20493 TTTAGGGGATTGGTTAGAGTT bast38D0707 CACCGTATATGCTGGTATTTC 20494 CACCGTATATCCTGGTATTTC baal19k05 TGAATCAACTGCAAATCTGAG 20496 TGAATGAACTCCAAATCTGAG baal19k05 TGTTGCAGGCAAAGTAAACCT 20497 TGTTGCAGGCGAAGTAAACCT baal19k05 CATGGTGCCACAAACAAGTAT 20498 CATGGTGCCATAAACAAGTAT baal19k05 TTACACGAAGTATAGCTTCAT 20499 TTACACGAAGAATAGCTTCAT baal19k05 TGAAGCAAGTATGAGATTGTC 20500 TGAAGCAAGTTTGAGATTGTC BaH19C21 CACAATGATGTGATCTGCTAA 20501 CACAATGATGCGATCTGCTAA bah49g10 GTCTAGAGTGGAGAAATACCA 20502 GTGTAGAGTGAAGAAATACCA bah49g10 CCGTAAGCCATACTTGCAAAT 20503 CCGTAAGCCAAACTTGCAAAT

TABLE 26-10 Haruna Nijo SEQ ID Clones H602  NO: bah49g10 GCTTGAATTTGAGGAGAGCAG 20504 GCTTGAATTTAAGGAGAGCAG bah49g10 AAGCAAGCCGTTGAACACAAT  20505 AAGCAAGCCGGTGAACACAAT bah49g10 TCGTGAGATCGCTGGATCTCT 20506 TCGTGAGATCCCTGGATCTCT bah49g10 CATCTTGCAATGTGCGTGCCC 20507 CATCTTGCAACGTGCGTGCCC bags23g18 AGAGCCTTGCGCAGTGCCTCC  20509 AGAGCCTTGCACAGTGCCTCC bags23g18 TTGTAATGATTTACAATTTTG 20510 TTGTAATGATGTACAATTTTG bags23g18 TTTTGCACATCTGATTGCATC 20511 TTTTGCACATTTGATTGCATC bags22o22 TGCACTATACAGTAAAAGCCA 20512 TGCACTATACGGTAAAAGCCA bags22o22 GTGTACAACTACTCCCATCAT 20513 GTGTACAACTGCTCCCATCAT bags22o22 AACACACATTGTTTTATTCGA  20514 AACACACATTTTTTTATTCGA bags22o22 ACTTCTTAGAGGGAATGTCTT  20515 ACTTCTTAGAAGGAATGTCTT baal20a03 CTGGTCGCAAGCGGCGCTATA 20516 CTGGTCGCAAACGGCGCTATA baal20a03 GTGTTGGCACAGGCAGCGGCA 20517 GTGTTGGCACCGGCAGCGGCA bags5e24 GAAATGTTTTTCGTCACTATT 20518 GAAATGTTTTCCGTCACTATT bags5e24 GTAGGCCAGCAGCTGCTTCTG 20519 GTAGGCCAGCGGCTGCTTCTG bags5e24 CCTGTTTGTAAGATAACTAGG  20520 CCTGTTTGTAGGATAACTAGG bags5f11 TACCTGGCTTAATGATCTCAG 20521 TACCTGGCTTCATGATCTCAG bags5f11 TGCCCTCGTCGGCGTTAATCA 20522 TGCCCTCGTCAGCGTTAATCA bags5f11 TGCTGTCCGTCACCCCCAAA 20523 TGCTGTCCGCCACCCCCAAA BaAK33B10 GAAAAAAAGAATATGGCAGCC 20524 GAAAAAAAGAGTATGGCAGCC BaAK33B10 GCAATCTTACAAACAAAATCA 20525 GCAATCTTACTAACAAAATCA BaAK33B10 AGAAATAGGTACTTCGATTAT  20526 AGAAATAGGTCCTTCGATTAT BaAK33B10 GATGTATGAAGAACCTTGGTA 20527 GATGTATGAAAAACCTTGGTA BaAK33B10 GTACGGACTATAACGTTGCGC 20528 GTACGGACTAAAACGTTGCGC BaAK33B10 GCTTCTAGTTCTGTTGGTGAG  20529 GCTTCTAGTTTTGTTGGTGAG

TABLE 26-11 Haruna Nijo SEQ ID Clones H602 NO: BaAK33B10 GTCAATATGATAGAAATAGGT  20530 GTCAATATGAGAGCAATAGGT BaAK33B10 AGGCATGCCACACTGGAGTAT 20531 AGGCATGCCAAACTGGAGTAT baal39j02 AGAGAGCATGGACAGGCATCG  20537 AGAGAGCATGAACAGGCATCG BaSD27H14 ATGTCCGCGGTGTCGTCCAGG 20538 ATGTCCGCGGGGTCGTCCAGG BaSD27H14 CGAAGACCTTTGCCTCAGGGT 20539 CGAAGACCTTCGCCTCAGGGT bah50m01 GGATGGACTACCTCAACCCGG 20540 GGATGGACTATCTCAACCCGG BaSD24E13 GGGGATCTTTCACCACAAACT  20541 GGGGATCTTTTACCACAAACT bah28k24 CATAGACGATTCAATATTAGA  20546 CATAGACGATACAATATTAGA bah28k24 ACACAACACCCGAGTGCACAC 20547 ACACAACACCTGAGTGCACAC bah47c11 GACAGTTGTTTCATTAGAATT 20548 GACAGTTGTTCCATTAGAATT bah47c11 TTAGGGCAGACAGTATGGTTG  20549 TTAGGGCAGAAAGTATGGTTG basd26m15 GGCATGCCACGATCCACATAT 20550 GGCATGCCACAATCCACATAT basd26m15 CTTAATAATAAGGCATGCATC  20551 CTTAATAATACGGCATGCATC bah60o02 CAAAAATGTTTGAGCTCCGTC 20552 CAAAAATGTTGGAGCTCCGTC bah60o02 CGTCAGCGATTAGGATTTCAT 20553 CGTCAGCGATAAGGATTTCAT bah60o02 GTACTGTATATTGTCACCGTA  20554 GTACTGTATACTGTCACCGTA bah50g06 GGGGGGATTTGTGGICCTGATG 20555 GGGGGGATTTCTGGCCTGATG bast15g0913 AGTACATCAGCAGCTAAAGCA  20556 AGTACATCAGTAGCTAAAGCA baall0n23 GACAGAAATGGTCGAACCTGT  20557 GACAGAAATGATCGAACCTGT baall0n23 TGACAAAGCATACAGCACTCC 20558 TGACAAAGCAAACAGCACTCC baall0n23 TATCACAGCCGAAATGAGCCC  20559 TATCACAGCCAAAATGAGCCC bags20c12 TATCAGACTTCTAATTTCTAC  20560   TATCAGACTTTTAATTTCTAC basd16f13 CCCGGGCGTCGGGGACTATCT  20561 CCCGGGCGTCCGGGACTATCT bags22p08 TGGCATTAGATGAGAAAAAGA 20564 TGGCATTAGACGAGAAAAAGA BaH23N06 AGATGGAGGCCAGCAGCACGG 20565 AGATGGAGGCTAGCAGCACGG

TABLE 26-12 Haruna Nijo SEQ ID Clones H602  NO: bags29c08 AATGAAGCCGACTCAAGCACT  20566 AATGAAGCCGGCTCAAGCACT bags29c08 ATCAGCGCCCCTTCTTCTACC 20567 ATCAGCGCCCTTTCTTCTACC bags11o23 GGTCAACTAGCTTCGTGTTCC  20568 GGTCAACTAGGTTCGTGTTCC baal18j13 ACATTGCTTCTTTACCCATGA 20571 ACATTGCTTCCTTACCCATGA baal18j13 ACCTCTCTCAGGCCCAAGCTC  20572 ACCTCTCTCAAGCCCAAGCTC baal18j13 TAAGTACTCCCTCCATCCCAA 20573 TAAGTACTCCATCCATCCCAA baal18j13 TAAAGGGTTATTTCCATTGAC 20574 TAAAGGGTTAATTCCATTGAC baal18ji3   ATGAAGGCCAGCAACGGGCAA 20575 ATGAAGGCCAACAACGGGCAA baal18j13 TTAAGTGACTCAACTTTGTAC  20576 TTAAGTGACTGAACTTTGTAC bah15m02 ACTTCTTCACAATGCAGTTCC  20577 ACTTCTTCACGATGCAGTTCC kr68b1103 CCCTTTCGTTTTGACTTCCCT 20580 CCCTTTCGTTATGACTTCCCT kr68b1103 GAGCAAGGTATGTGTTTCCAC  20581 GAGCAAGGTACGTGTTTCCAC bags15b10 ACACTATACTCTGCAATCTGA  20583 ACACTATACTTTGCAATCTGA bags20h21 GAGCAATTCAGAGACCACAGA  20584 GAGCAATTCAAAGACCACAGA BaAK29K06 GCTGGGTGCAGGGAGTGATGA  20585 GCTGGGTGCATGGAGTGATGA BaAK29K06 TGTAGATAGCATGGACATGAG  20586 TGTAGATAGCCTGGACATGAG BaAK29K06 TTGTGGTTATGGAGATGGTGG  20587 TTGTGGTTATAGAGATGGTGG BaAK29K06 ATGACATGAAGAGGAGCAATT  20588 ATGACATGAATAGGAGCAATT BaAK29KO6 CTAGGAAAATAAATOGGGAAC  20589 CTAGGAAAATGAATGGGGAAC bah56j14 CTTCATGCCTGTGACATCTTT  20591 CTTCATGCCTATGACATCTTT bah56j14 TTGCCATCCAGCCTGCAGGGT  20592 TTGCCATCCATCCTGCAGGGT bah56j14 CTGCAGCTGTCTTGTCCTGTT  20593 CTGCAGCTGTTTTGTCCTGTT bags3j24 CGTGGGGGTCAGCCGGCATGA  20595 CGTGGGGGTCGGCCGGCATGA bags38m08 AGACTTTACTTTGGTAGTAAT 20596 AGACTTTACTCTGGTAGTAAT bags35g06 GTCTCTCCATCAACCTCAATG  20598 GTCTCTCCATTAACCTCAATG

TABLE 26-13 Haruna Nijo SEQ ID Clones H602  NO: bags35g06 GATCCCAGGCATGGAAGTTGT 20599 GATCCCAGGCTTGGAAGTTGT bags37b01 GAACTGCCTCTGAGCTCTTTA 20600 GAACTGCCTCAGAGCTCTTTA bags37b01 GGTCGCCGTCATCAGGTGTTG 20601 GGTCGCCGTCGTCAGGTGTTG BaAL19F02 CCAAGCAACAGTTATCTCCAC 20602 CCAAGCAACATTTATCTCCAC BaAL19F02 TCAAAACCTTCCTGACTTATG 20603 TCAAAACCTTTCTGACTTATG BaAL19F02 TGCTGAATTTGCTTGGAAAGA 20604 TGCTGAATTTACTTGGAAAGA BaAL19F02 TTTACAGATAGAACTTGCCTC 20605 TTTACAGATACAACTTGCCTC BaAL19F02 CTAGCCTTCCTCCTGATTTTA 20606 CTAGCCTTCCACCTGATTTTA baal4d09 ATTGTACCAAATTGGGTAAGT 20607 ATTGTACCAAGTTGGGTAAGT bags27f15 CCAGCAGTTACATATGACATG 20610 CCAGCAGTTATATATGACATG bags27f15 ATCATGCTAGCCATTTTGGGC 20611 ATCATGCTAGTCATTTTGGGC bags10k14 ACCACAAAATGGATTTATGTT 20612 ACCACAAAATTGATTTATGTT bags10k14 AGTTTGATAGTTCACTGGCTA 20613 AGTTTGATAGATCACTGGCTA baak38n21 CGATAGTGGACGGCCGGAAGA 20615 CGATAGTGGATGGCCGGAAGA baak38n21 ACATTGTCAATCTCCAGGCCT 20616 ACATTGTCAACCTCCAGGCCT baak38n21 TTCAAGTTGCAAAGCAAGCCG 20617 TTCAAGTTGCGAAGCAAGCCG baak38n21 TCCAGCCAGGAAAACACCGTT 20618 TCCAGCCAGGGAAACACCGTT baale38n21 ACTTATTCATTGTGGATACGT 20619 ACTTATTCATCGTGGATACGT baak30e05 GGTCTGAATTGTGGGCAGGCA 20620 GGTCTGAATT-TGGGCAGGCA 20621 bastl04a0101 TACGACGACGGGCAGCCTTGG 20622 TACGACGACGCGCAGCCTTGG baak28p18 ATAGGCTTTTCTCCTGCCCAA 20623 ATAGGCTTTTGTCCTGCCCAA BaGS34E01 AAGACGGGCAAAACAAGAGGT 20624 AAGACGGGCAGAACAAGAGGT BaH54H04 AGTCTTGATATGATGAAACTT 20625 AGTCTTGATACGATGAAACTT bah13o19 TTGGTATTAGGGTTTCAAAAA 20626 TTGGTATTAGAGTTTCAAAAA baht3o19 AAATTCCCCAGTGGGAAAACA 20627 AAATTCCCCAATGGGAAAACA

TABLE 26-14 Haruna Nijo SEQ ID Clones H602  NO: BaSD27A15 AGCAACTCCAGCATAGCGTTT 20631 AGCAACTCCAACATAGCGTTT BaSD27A15 CTAATATAATCTGGTGCGCAG 20632 CTAATATAATTTGGTGCGCAG BaSD27A15 AATGCCCTCGCCTTCCAGCCC 20633 AATGCCCTCGGCTTCCAGCCC BaSD27A15 TGCTGTCCAGGAGCGTCTCCT 20634 TGCTGTCCAGTAGCGTCTCCT BaSD27A15 CCATCCTGCAAAATTCCTGTC 20635 CCATCCTGCAGAATTCCTGTC BaSD27A15 CGGTAATAATTGATGCTGTCC 20636 CGGTAATAATCGATGCTGTCC BaSD27A15 GCGTGGTAGGCGAGTTCTGAT 20637 GCGTGGTAGGAGAGTTCTGAT bags9n02 AGCACATACAGACCCCTCGGA 20639 AGCACATACATACCCCTCGGA bastl30A0202 CTGAGTGCAATAAACATAACA 20642 CTGAGTGCAAGAAACATAACA bastl30A0202 TGGAAGAAAATACAACAAAAT 20643 TGGAAGAAAAAACAACAAAAT bastl30A0202 TGGACGAACGTAACTGCGGCG 20644 TGGACGAACGCAACTGCGGCG bags22k12 CACTTTGGACCCTAAATGCCC 20646 CACTTTGGACACTAAATGCCC bags22k12 ACATTAATACGATGATCAGCT 20647 ACATTAATACTATGATCAGCT bags22k12 CGAAATCACAGGATAGATGAA 20640 CGAAATCACATGATAGATGAA baak13g12 GGACGGGTACATCTTCCTCAG 20649 GGACGGGTACTTCTTCCTCAG baak13g12 CGGCGGCTCCGAACACGGAGC 20650 CGGCGGCTCCAAACACGGAGC baak13g12 CGACGCGGCCGCGTCGATGGT 20651 CGACGCGGCCACGTCGATGGT bags17j17 AGGTCTTTGCATCACGCCTGG 20654 AGGTCTTTGC-TCACGCCTGG 20655 bags17j17 CGTCTTTGTAGAAGGCACAGC 20656 CGTCTTTGTA-AAGGCACAGC  20657 baal12j15 TACTAACTTCTTACATGGCAA 20658 TACTAACTTCCTACATGGCAA baal12j15 ATGGCAATCTCCAAGCAGTGT 20659 ATGGCAATCTTCAAGCAGTGT baal12j15 AAAGGGGGCGGCGGGTTGACA  20660 AAAGGGGGCGACGGGTTGACA bah34j11 CTTCTCTCCGAAGGGCGTCAA 20662 CTTCTCTCCGGAGGGCGTCAA bah34j11 CTGATGATGTCTTGGCAAGGA 20663 CTGATGATGTTTTGGCAAGGA BaGS30I11 GGAGTGCCTCTGGTACCTCGT  20664 GGAGTGCCTCCGGTACCTCGT

TABLE 26-15 Haruna SEQ ID Clones Nijo H602 NO: BaGS30I11 CCATCCTCCTCTTCGTGGGCT 20665 CCATCCTCCTATTCGTGGGCT BaGS30I11 TCTACCGAGAGGAATGGATCG 20666 TCTACCGAGACGAATGGATCG baal18m24 CTGCAGCCCCCTGCCTNCAGA 20668 CTGCAGCCCC-TGCCTNCAGA 20669 baal18m24 TGCTCCAGCGGCTTGACCACC 20670 TGCTCCAGCGNCTTGACCACC baal18m24 TTGAGCTTGGCCACCTCGATG 20671 TTGAGCTTGGGCACCTCGATG baak15l06 TGCGGGAGGCACGCGTGAGGA 20672 TGCGGGAGGCGCGCGTGAGGA baak15l06 CATTATTCTTATCATCAAATC 20673 CATTATTCTTTTCATCAAATC bald18o14 GATGCTTCTCTCCTGGCTGTA 20674 GATGCTTCTCACCTGGCTGTA bald18014 ATGGGTTGGCAAGCAATCTTT 20675 ATGGGTTGGCGAGCAATCTTT bags24p22 GCATTTGGTGGCATTGAGCTG 20676 GCATTTGGTGACATTGAGCTG bags24p22 GCACTCCTATGACAGCACACA 20677 GCACTCCTATCACAGCACACA bags24p22 GGAGAAGAACAACAAAAAGCA 20678 GGAGAAGAACGACAAAAAGCA baal37a09 TGTTGCTGTTAGTTTCAGAGC 20679 TGTTGCTGTTGGTTTCAGAGC bags5l01 TTTAGGTCAACAATCATCTAA 20680 TTTAGGTCAATAATCATCTAA bags5l01 TCTGCTTTTCGAAGAGATGGT 20681 TCTGCTTTTCAAAGAGATGGT BaAK12P03 GGCATGCGACGTCACTCCCGT 20682 GGCATGCGACATCACTCCCGT BaAK12P03 CCACCAGCTGCTTTCCGACGT 20683 CCACCAGCTGTTTTCCGACGT baak21 f10 CGGAAAATGCAGGANCNAGGA 20684 CGGAAAATGCNGGANCNAGGA bastl30e0410 ATGGAAATTCTCCCACATCTT 20685 ATGGAAATTCGCCCACATCTT kr25c0206 CAATGGCGAAGACTCTGCCAT 20686 CAATGGCGAAAACTCTGCCAT kr25c0206 AAAAAGCTGATAAAGCGCCAG 20687 AAAAAGCTGACAAAGCGCCAG bah54n06 TTTCCCAAAAGAAACGCCAAA 20690 TTTCCCAAAACAAACGCCAAA bast22F0311 ATGAAATGACCTGACCAGCTA 20692 ATGAAATGACGTGACCAGCTA bast22F0311 ACTGCAATCGATAGTACTACA 20693 ACTGCAATCGGTAGTACTACA bast22F0311 TCGGAGGCTCATCAGATCAGA 20694 TCGGAGGCTCGTCAGATCAGA

TABLE 26-16 Haruna SEQ ID Clones Nijo H602 NO: basd1 m04 CGCATCTTCCACACGGCGGAA 20695 CGCATCTTCCGCACGGCGGAA basd1 m04 AGTAGAGTGCAAAGTCCTCGC 20696 AGTAGAGTGCCAAGTCCTCGC basd1 m04 TTTCTTACTCCTATGTCGGTG 20697 TTTCTTACTCATATGTCGGTG baak41 d01 GATAGTTACT-TCTGTTATAG 20699 GATAGTTACTGTCTGTTATAG 20700 baak41 d01 TCTTANGAGCAAATATCTTCA 20701 TCTTANGAGCNAATATCTTCA baak41 d01 TTTGGCTATGGTCCACTCCAT 20702 TTTGGCTATGATCCACTCCAT baak13j10 TGTTATACACGCGACAAAGAA 20704 TGTTATACACACGACAAAGAA baak44h11 AACTGTAATCGGAGAGATGCA 20705 AACTGTAATCAGAGAGATGCA baal19j09 GAAAGTCAGANGAGACCATCA 20706 GAAAGTCAGAGGAGACCATCA baal19j09 GGTTCTTGGCAGACTCCGGAC 20707 GGTTCTTGGC-GACTCCGGAC 20708 baet37C1105 ATGCTTGTTATTGAAGCTAGA 20709 ATGCTTGTTACTGAAGCTAGA baet37C1105 CCTCCCTGTCGACCGGCATGT 20710 CCTCCCTGTCTACCGGCATGT basd23f10 AAAGGAACTGCTACGGCGTCA 20712 AAAGGAACTGTTACGGCGTCA basd23f10 TCCAGTATGGTCGGTACTTGG 20713 TCCAGTATGGGCGGTACTTGG bah41 p07 TGAGAACCTCGACGATCTCGC 20714 TGAGAACCTCAACGATCTCGC bah4l p07 CCACAGAGGCTACCTCGGTCT 20715 CCACAGAGGCCACCTCGGTCT baak1g13 TACACTCTAAAGATGTCTACT 20716 TACACTCTAAGGATGTCTACT baak1g13 CCGCAATTGAGATAGGGCTCC 20717 CCGCAATTGAAATAGGGCTCC baak1g13 ATAAATGTGCACTAGGAAATT 20718 ATAAATGTGCGCTAGGAAATT baak1g13 TGCGACTCGGTTCCTATGGTG 20719 TGCGACTCGGCTCCTATGGTG baak1g13 AGTTATGTGACAAGAAAAGGA 20720 AGTTATGTGAGAAGAAAAGGA baak1g13 AGTGCTCACAACAACTCTCCG 20721 AGTGCTCACATCAACTCTCCG bags22l14 GCTCACTCTCACGCCGAAGCT 20723 GCTCACTCTCGCGCCGAAGCT bags22l14 TCAAGAAGCAAAGCGGAAGTA 20724 TCAAGAAGCAGAGCGGAAGTA bags22l14 TCTTCTTCCCGGGGACGTGGA 20725 TCTTCTTCCCAGGGACGTGGA

TABLE 26-17 Haruna SEQ ID Clones Nijo H602 NO: bags22l14 ATGAACAGCATACTCATCACA 20726 ATGAACAGCAAACTCATCACA bast26E1210  AGCTCCTGCCACATTATTGCC 20727 AGCTCCTGCCGCATTATTGCC baet25f0911 ACTTGCCGGGGACGAAGTTGG 20728 ACTTGCCGGGAACGAAGTTGG baet25f0911 TGGTGGCGAAGGCCCAGGCGT 20729 TGGTGGCGAAAGCCCAGGCGT BaAK32N13 TCACATGCAACATCTATCAGT 20730 TCACATGCAAAATCTATCAGT BaAK32N13 ACTTGCCGGGGACGAAGTTGG 20731 ACTTGCCGGGAACGAAGTTGG BaAK32N13 TGGTGGCGAAGGCCCAGGCGT 20732 TGGTGGCGAAAGCCCAGGCGT BaAK32N13 CGAAGAAGCCAAACATGGAGA 20733 CGAAGAAGCCGAACATGGAGA bags31p01 AGCCTCCGAAGCCGTAGAGGG 20734 AGCCTCCGAATCCGTAGAGGG bags31p01 TGAGCACGCCCCGGGTCTCCT 20735 TGAGCACGCCGCGGGTCTCCT bags31p01 CGTCCATGGCGGTGACGGTCT 20736 CGTCCATGGCTGTGACGGTCT bags31p01 GGCGCTTGGCGCCGCCCTTGC 20737 GGCGCTTGGCACCGCCCTTGC baak30m11 CTACGCCTACTAATGTTCCAT 20738 CTACGCCTACCAATGTTCCAT baak30m11 TGTTCCATCCATTCATTCTTG 20739 TGTTCCATCCGTTCATTCTTG baak30m11 GTACAATTGTTTGGATCAGTG 20740 GTACAATTGTGTGGATCAGTG bastl46f1012 TGCAGCCCAATGTTTGGCATC 20741 TGCAGCCCAAGGTTTGGCATC bastl46f1012 ACACTCATCACCCTACCGACA 20742 ACACTCATCAACCTACCGACA bags3e06 TCCCAAAGAAGAAGAGGGCCT 20743 TCCCAAAGAAAAAGAGGGCCT bags3e06 ATATGATACATTAGGCATCAC 20744 ATATGATACACTAGGCATCAC baak46o19 CAATGCTCACTTTATCTATGT 20749 CAATGCTCACCTTATCTATGT baak46o19 GCAGCTGCAGCAAGCCATCTT 20750 GCAGCTGCAG-AAGCCATCTT 20751 baak46o19 GTACCCTTTTNCATATGTTGG 20752 GTACCCTTTTCCATATGTTGG basd3c19 ACGACAATGGCAACCAACTTT 20753 ACGACAATGGGAACCAACTTT basd3c19 GAATCAATGGGAACCAACTTT 20754 GAATCAATGGTAACCAACTTT bags30f01 CATCACCATCGTCACTCCCTG 20755 CATCACCATCATCACTCCCTG

TABLE 26-18  Haruna SEQ ID Clones Nijo H602 NO: bags30f01 CCTCAGGGTCGTCGTCCTCAT 20756 CCTCAGGGTCATCGTCCTCAT bags32d08 CAACAGGGGCCTGATCCCCAC 20758 CAACAGGGGCATGATCCCCAC bags32d08 ATCATCGGTTTGTCATAAACT 20759 ATCATCGGTTCGTCATAAACT BaAK23M23 GCACGCCACACAGTCCATATG 20760 GCACGCCACAGAGTCCATATG BaAK23M23 GGCTCATCACACACAGGATAC 20761 GGCTCATCACGCACAGGATAC bah21a16 GGTGCTCCTCTGACCACCACG 20762 GGTGCTCCTCCGACCACCACG baal3m08 GGCTCATCACACACAGGATAC 20763 GGCTCATCACGCACAGGATAC BaAK21I09 TATTTGAATCGCAAACAAACA 20764 TATTTGAATCACAAACAAACA baet33a0301 GCCGGCATGAGAAGCTTCCCC 20765 GCCGGCATGAAAAGCTTCCCC baet33a0301 TATTGAAATTCTAAAAATGTG 20766 TATTGAAATTTTAAAAATGTG baet33a0301 CCGCTTATTTATTTTTTCTCC 20767 CCGCTTATTTTTTTTTTCTCC baet33a0301 ACACACAAACGAGTGCCGGTT 20768 ACACACAAACAAGTGCCGGTT baet33a0301 TGGCCATGACGATGTTATCGG 20769 TGGCCATGACAATGTTATCGG baet33a0301 TCAGTCTTCAGAGCGCGGCTT 20770 TCAGTCTTCAAAGCGCGGCTT baet33a0301 TCTCCTCCACGAACTTGTCCA 20771 TCTCCTCCACAAACTTGTCCA baak24k02 TCAAATGATTTCTAAACTAAC 20772 TCAAATGATTCCTAAACTAAC baak24k02 TGCCATGCTCGCTGAACAGCT 20773 TGCCATGCTCACTGAACAGCT bast75d1208 GTGTGGCAGCAGCACTGGAGG 20775 GTGTGGCAGCGGCACTGGAGG bag34f06 ACATTATGGTGTGCCGGCAAC 20776 ACATTATGGTATGCCGGCAAC bags34f06 GTGTGGCAGCGGCACTGGAGG 20777 GTGTGGCAGCAGCACTGGAGG bags6f09 CACCGTGCCTCATTGTACACT 20778 CACCGTGCCTGATTGTACACT bags6f09 AGGGCCTCTATTATGGCATCT 20779 AGGGCCTCTACTATGGCATCT bags6f09 ACAGAAAAGACAATCAGCCTT 20780 ACAGAAAAGATAATCAGCCTT bags6f09 TCTTCTCTCAAAGAACTGCCA 20781 TCTTCTCTCAGAGAACTGCCA bags6f09 TCTGATAGCTTAAAAAATGTA 20782 TCTGATAGCTGAAAAAATGTA

TABLE 26-19 Haruna Nijo SEQ Clones H602  ID NO: bags6f09 TCAAAGACATCGAGCCTTGTT 20783 TCAAAGACATAGAGCCTTGTT bags7b10 GAGATTCTTGTGATCCCCTCT 20784 GAGATTCTTGCGATCCCCTCT bags7b10 TGATTAAACAAAATATCAGTA 20785 TGATTAAACAGAATATCAGTA bags7b10 AAAGTTTCCATAATAACAACA 20786 AAAGTTTCCACAATAACAACA bags7b10 TCTACAAAGTTCACTATCCAT 20787 TCTACAAAGTCCACTATCCAT bags7b10 GTGGCTAATCCCTAATCCTGC 20788 GTGGCTAATCACTAATCCTGC bags37e01 AGCTCTGGGGTGCAGATTTCC 20791 AGCTCTGGGGCGCAGATTTCC bags37e01 GTCATTCATCTTCTCTTTTGA 20792 GTCATTCATCCTCTCTTTTGA BaGS24M06 GTTTGCCGACCATACCTCTTG 20794 GTTTGCCGACAATACCTCTTG BaGS24M06 CGTCATCCTCATCATCGTCGG 20795 CGTCATCCTCGTCATCGTCGG BaGS24M06 ACCTTCTTGTGAACTGCTTCC 20796 ACCTTCTTGTAAACTGCTTCC BaGS24M06 GCTAACAACAGGGTTGAAACA 20797 GCTAACAACAAGGTTGAAACA bags22m23 AATGCCTAGCAATCAGAATCA 20798 AATGCCTAGCCATCAGAATCA bags22m23 TCTGCAAGAATTGTATTTCTA 20799 TCTGCAAGAACTGTATTTCTA bags22m23 TTAAGTTCCGTAAAAATGTAC 20800 TTAAGTTCCGCAAAAATGTAC bags22m23 TGGCTGGCGTTTGACATATCC 20801 TGGCTGGCGTCTGACATATCC bags22 m23 CCCATGAAAGCAGCTCCAGTC 20802 CCCATGAAAGAAGCTCCAGTC basd18d18 AGTAAATGCAGACTTCTGATA 20803 AGTAAATGCAAACTTCTGATA basd18d18 GCATTAAGCACACCTCTCCCG 20804 GCATTAAGCAAACCTCTCCCG basd18d18 GGATTAGCAACCGAACACGAT 20805 GGATTAGCAAGCGAACACGAT bastl06F0212 CCAGATCATCGACTTCAGCGG 20806 CCAGATCATCAACTTCAGCGG BaH49O16 TACTAGTTTTGTATATAAAAC 20807 TACTAGTTTTTTATATAAAAC BaH49O16 ATATAAAACGTTCACGTAGCA 20808 ATATAAAACGGTCACGTAGCA BaH49O16 AGGTCATTGCGCGGCCCTTCG 20809 AGGTCATTGCACGGCCCTTCG BaH38 N06 TTGAGACGGAGGAAGTATTAC 20811 TTGAGACGGATGAAGTATTAC

TABLE 26-20 Haruna Nijo SEQ Clones H602  ID NO: BaH38N06 ACTTATATAGCACTCCCTCCG 20812 ACTTATATAGTACTCCCTCCG bah56p16 AACTGAAAACTCCAGCCTTCC 20813 AACTGAAAACCCCAGCCTTCC basd13o13 ACTGTGATGGCTCAGCAGCCT 20814 ACTGTGATGGTTCAGCAGCCT bah34f11 TCATACGACCCGACACCTAAA 20816 TCATACGACCTGACACCTAAA bags37g04 TTGCTCTTACAATCGTATTTA 20817 TTGCTCTTACTATCGTATTTA bags37g04 CGAGCTGCTTCGTCCAGTCGA 20818 CGAGCTGCTTAGTCCAGTCGA baak43o03 CGGGGATGATGTAGTCTTTAT 20820 CGGGGATGATATAGTCTTTAT BaAL4J21 GGCAATGCTGTTCTTTGTAGT 20821 GGCAATGCTGCTCTTTGTAGT BaH51J22 GCCCAGACGTCCCCCAACACG 20822 GCCCAGACGTTCCCCAACACG BaH51J22 TTTTATCTGGGGAAGGATAAA 20823 TTTTATCTGGAGAAGGATAAA BaH51J22 CTGAGCTGAAAGTGCAGCAGC 20824 CTGAGCTGAAGGTGCAGCAGC BaH51J22 GTTCCTTGGTAGTCAATCGTC 20825 GTTCCTTGGTCGTCAATCGTC bah58103 TAGCACCAACGCGGCCATTCG 20826 TAGCACCAACACGGCCATTCG BaGS21M18 TCAGGTTGTCACCGAACGTCT 20827 TCAGGTTGTCGCCGAACGTCT bags31k06 AAAGATCTGGAGGCGAGGCTC 20828 AAAGATCTGGGGGCGAGGCTC bags3lk06 TAGTATAGCCACACTACTGAC 20829 TAGTATAGCCCCACTACTGAC bags3lk06 TGTCCCTTGCAAAGCAGGCTG 20830 TGTCCCTTGCGAAGCAGGCTG bags3lk06 GGACACCACACAGAGTTAGAT 20831 GGACACCACAGAGAGTTAGAT baal4o01*VRN  CTCTCGCCTGCCGCCGCTGGG 20832 2(sh2) CTCTCGCCTGTCGCCGCTGGG baal4o01*VRN  ACACCAATTCAAAAGATGGTT 20833 2(sh2) ACACCAATTCGAAAGATGGTT baal4o01*VRN  CCATCCACAGTGGAAGCGCCG 20834 2(sh2) CCATCCACAGGGGAAGCGCCG baal4o01*VRN  ACTACTTTAGTAATCTAAACG 20835 2(sh2) ACTACTTTAGCAATCTAAACG baal4o01*VRN  CTGCGACAGGCGGAGCATCCC 20836 2(sh2) CTGCGACAGGGGGAGCATCCC bah39o04 CCGTGTAGATACCGTCGCGCT 20837 CCGTGTAGATGCCGTCGCGCT bah39o04 CCACGATCTGCCCCTCGGAGA 20838 CCACGATCTGGCCCTCGGAGA

TABLE 26-21 Haruna Nijo SEQ Clones H602  ID NO: bah39o04 GTAGATCACATAAATTTTGTT 20839 GTAGATCACACAAATTTTGTT bast14h0416  CATCATGATACATGTCTTGGT 20841 CATCATGATATATGTCTTGGT bastl44H115 GCCGGCCCGACGGAGCTCGTC 20843 GCCGGCCCGATGGAGCTCGTC bags23f02 CGCAGAAACGGCTCTAACAAC 20849 CGCAGAAACGACTCTAACAAC bastl40e1010 GGTTCAGAACGGTGGAGGATT 20850 GGTTCAGAACCGTGGAGGATT bah15e16 CATGGTCAAACCTTTGTGCCC 20856 CATGGTCAAAACTTTGTGCCC basd27o16 TCGCTCACCCTGCGGAACATC 20858 TCGCTCACCCGGCGGAACATC basd27o16 TCCATCTCGTACATGCCCTCG 20859 TCCATCTCGTCCATGCCCTCG basd27o16 ACTCGGCGACAAGGTCGTTCA 20860 ACTCGGCGACGAGGTCGTTCA bald 27016 GAGCOGAGCGAATCCGTTGCC 20061 GAGCGGAGCGCATCCGTTGCC basd27o16 CATATAGCTGAACGGAGCGGA 20862 CATATAGCTGCACGGAGCGGA kr66g0713 TTTATGATCATGAATTTACAA 20863 TTTATGATCACGAATTTACAA kr66g0713 TATCTTGAAGGGTCCCAAATT 20864 TATCTTGAAGTGTCCCAAATT baak46c17 GAAGCCATGAGTTCATTCATT 20865 GAAGCCATGATTTCATTCATT baak46c17 AATACCTTGTCTACAGAGTAC 20866 AATACCTTGTTTACAGAGTAC baet19C1206 TACACAAACAGACACAAGGCT 20867 TACACAAACAAACACAAGGCT BaGS26G20 GCAATGAACAATGCCAGTGCC 20870 GCAATGAACACTGCCAGTGCC BaGS26G20 AGAGGATCTTTTCAGGCACAC 20871 AGAGGATCTTCTCAGGCACAC bags28c14 GGCCATCACACGATGAATGCC 20872 GGCCATCACAGGATGAATGCC bags28c14 GAATAAAAAGTCTCAGTCCAA 20873 GAATAAAAAGGCTCAGTCCAA bags28cl4 TGCTCTCTCACTCGGGGTGAT 20874 TGCTCTCTCAGTCGGGGTGAT kr61g1214 AAGATTTCGTCGAATAGCCTG 20875 AAGATTTCGTTGGATAGCCTG kr61g1214 GATTTCGTCGAATAGCCTGAA 20876 GATTTCGTTGGATAGCCTGAA bags9g08 CAAATATATGTGATTGATGCA 20877 CAAATATATGGGATTGATGCA bags9g08 TGAAGATGCACATATTACTAA 20878 TGAAGATGCAAATATTACTAA

TABLE 26-22 Haruna Nijo SEQ ID Clones H602 NO: bags9g08 GGGAAAGAGCGGCCAAAAGTT 20879 GGGAAAGAGCAGCCAAAAGTT bags5p01 CTGGAGATCAACATGAATGAT 20880 CTGGAGATCAGCATGAATGAT bags5p01 AGATATCTGTGTTCTTGACAT 20881 AGATATCTGTATTCTTGACAT bags5p01 AGTTTTTTCAAGAGCTGCGAC 20882 AGTTTTTTCAGGAGCTGCGAC bags5p01 TGGAAGTTCACATCAAGGACA 20883 TGGAAGTTCAAATCAAGGACA baal4d04 CGGGGAAAACAACCGTGAAGT 20884 CGGGGAAAACCACCGTGAAGT baal4d04 CACTGGTCCAGGCTCAGCGAG 20885 CACTGGTCCAAGCTCAGCGAG baal4d04 GCTCCATGTCCGTGTACTGCG 20886 GCTCCATGTCTGTGTACTGCG baal4d04 CAATGTCTATGTCGTAGGCCA 20887 CAATGTCTATATCGTAGGCCA baal4d04 GCTGCTGCTGCTGCCTCCGCA 20888 GCTGCTGCTGTTGCCTCCGCA bah11e02 CGGGGAAAACAACCGTGAAGT CGGGGAAAACCACCGTGAAGT bah11e02 AGAGGTCGTCGGCCTTCCGCT 20891 AGAGGTCGTCAGCCTTCCGCT bah11e02 CACTGGTCCAGGCTCAGCGAG 20892 CACTGGTCCAAGCTCAGCGAG bah11e02 TCAGTTTGAATATATACCAAA 20893 TCAGTTTGAACATATACCAAA bast41f0311 TGCTGTTGCTCTTGTTGGTGT 20894 TGTTGTTGCTTTTGTTGGTGT bast41f0311 GCTGTTGTTGCTGTTGCTCTT 20895 GCTGTTGTTGTTGTTGCTCTT bastl54d0307 CAGACTAGCTCAAATGGTGAA 20896 CAGACTAGCTGAAATGGTGAA kr33h0816 GGTAAACTAAACGGGGCATAT 20899 GGTAAACTAAGCGGGGCATAT kr33h0816 TTGCCTTGCCGTGCTGTTAGA 20900 TTGCCTTGCCTTGCTGTTAGA BaH38F16 GCTCTCATGCTGNGACCTCAA 20901 GCTCTCATGCGNNGACCTCAA BaGS21P09 TAAATCAAGCAGACGAGGCTC 20902 TAAATCAAGCGGACGAGGCTC BaGS21P09 CGGCAGCCCAGATGTACTCAT 20903 CGGCAGCCCACATGTACTCAT BaGS21P09 ATCAGATTCAGGAACAGGTTG 20904 ATCAGATTCATGAACAGGTTG basd21g02 GGCGACGGTGACGGCGGTGAT 20909 GGCGACGGTGGCGGCGGTGAT bah22m17 TTGTTCATTACACATACTAAC 20910 TTGTTCATTATACATACTAAC

TABLE 26-23 Haruna Nijo SEQ ID Clones H602 NO: bah22m17 TCAAGAACCCAACCTGGAGAA 20911 TCAAGAACCCGACCTGGAGAA bah22m17 ATCATGCTGCGACGGTCAGCT 20912 ATCATGCTGCAACGGTCAGCT bah22m17 TGTTTCCGGTATCTCTGCTGC 20913 TGTTTCCGGTGTCTCTGCTGC BaGS9H19 ATTATCACTTTAGTGGATAGT 20914 ATTATCACTTAAGTGGATAGT BaGS9H19 ATAGTGAATGAATGCGCAAAG 20915 ATAGTGAATGTATGCGCAAAG BaGS9H19 TGAGTGAGTTACTGAGATAAA 20916 TGAGTGAGTTGCTGAGATAAA bah26m24 ATTTTGAATTGTTTAAAATAT 20917 ATTTTGAATTATTTAAAATAT BaH56D01 AGCCATATCCGTCTGCAGTCA 20918 AGCCATATCCATCTGCAGTCA BaH56D01 GCGTACCATACAGGCGAGGCG 20919 GCGTACCATATAGGCGAGGCG BaH56D01 GATAATAGCCACCAACAGGAG 20920 GATAATAGCCGCCAACAGGAG BaH56D01 GGAGGTACACATGCAAAACCT 20921 GGAGGTACACGTGCAAAACCT BaH56D01 CTACACGCTTCCGTGTCAGCG 20922 CTACACGCTTTCGTGTCAGCG baal9m13 GTACACCTATGCTACAAGGAC 20926 GTACACCTATACTACAAGGAC baak21k16 CCTCNACGGCGCCCATGAGCA 20927 CCTCNACGGCACCCATGAGCA baak21k16 CNGGGTCGTCNGCAAGNCCGA 20928 CNGGGTCGTCGGCAAGNCCGA baak21k16 CTGGGTATAGNGGGTCAACAA 20929 CTOGGTATAGCGGGTCAACAA baak21k16 GAGCTCAGCAGAATGCCTCNG 20930 GAGCTCAGCA-AATGCCTCNG 20931 baak21k16 GTGGTCCTCCGGCAATGCGGT 20932 GTGGTCCTCCAGCAATGCGGT bags19o14 ACGGCATGCGGTCAGTGTCTC 20935 ACGGCATGCGTTCAGTGTCTC bags19o14 CCTTTGAGTTTGCCATACACT 20936 CCTTTGAGTTGGCCATACACT baak35m03 CTACGTATCTATCCTCGTACC 20937 CTACGTATCTTTCCTCGTACC baak35m03 TCAGCAGACAATGCGTGTACG 20938 TCAGCAGACAGTGCGTGTACG baak35m03 TGCCTCCTCTGGCGAGAGATC 20939 TGCCTCCTCTTGCGAGAGATC baak35m03 GTGCATTGTCACCCCGCCTTG 20940 GTGCATTGTCACCCCGCCTTG baak35m03 GCTACACTTTTTTGTTACAAA 20941 GCTACACTTTGTTGTTACAAA

TABLE 26-24 Haruna Nijo SEQ ID Clones H602 NO: baak35m03 TCCTTACTTAAACATAATGAA 20942 TCCTTACTTAGACATAATGAA baak33 o23 GATCATGGACGCAACATAAAC 20943 GATCATGGACACAACATAAAC kr57F1012 TACAACATGGTATCGTATCCT 20944 TACAACATGGCATCGTATCCT bags22l16 TTTAGCAGACCAAGTGAAGAG 20945 TTTAGCAGACAAAGTGAAGAG bags22l16 AAACATCGGCAGTCATGAACT 20946 AAACATCGGCGGTCATGAACT baet43C0505 TGTACGATGCATGTTAGAACG 20947 TGTACGATGCGTGTTAGAACG bastl22E1010 CCTTGGTCTTATTCGAATATA 20949 CCTTGGTCTTCTTCGAATATA BaH53N24 TACAGCAACCAAAGCTGGGCC 20950 TACAGCAACCGAAGCTGGGCC baal8e24 GGGGCTCTCATGGATGTCGGT 20951 GGGGCTCTCACGGATGTCGGT baal8e24 TCAAGTTTCCACTAGAGGCCC 20952 TCAAGTTTCCCCTAGAGGCCC BaH62H21 AGCTAGGAAGTAAATATGTGT 20953 AGCTAGGAAGCAAATATGTGT BaH62H21 GCCGGACCGGATGTGGAAGCC 20954 GCCGGACCGGCTGTGGAAGCC bah42k01 TGAAACTTAATTGAGGTAGAC 20955 TGAAACTTAACTGAGGTAGAC bah42k01 AGTAAATAAAACAGAATGCCT 20956 AGTAAATAAATCAGAATGCCT bah42k01 CATACTGCAGAGCCTATTTTA 20957 CATACTGCAGGGCCTATTTTA basd26p09 CCAGTCATCAACTATGTCTAT 20958 CCAGTCATCA-CTATGTCTAT 20959 basd26p09 ATCTATCCACCACCCAGTCAT 20960 ATCTATCCAC-ACCCAGTCAT 20961 basd26p09 GGTCCCGAGAATAGTGCGGAT 20962 GGTCCCGAGA-TAGTGCGGAT 20963 bags4p18 CAGGGTGGCTGTGTCCAGCTT 20964 CAGGGTGGCTATGTCCAGCTT bags4p18 AATGAAGATCACTCACAAGAA 20965 AATGAAGATCGCTCACAAGAA bags4p18 AACTGAGAAGTATGTTGGTTT 20966 AACTGAGAAGCATGTTGGTTT bast70d1107 ACCGGAGAAGCAGCAGCTGCG 20967 ACCGGAGAAGAAGCAGCTGCG bast70d1107 CCACTTCACCATTCTAATTAA 20968 CCACTTCACCCTTCTAATTAA bah26i23 CCTCGTAATCACGGCTTTCTG 20969 CCTCGTAATCGCGGCTTTCTG baal39g02 TCCTTGCTCAGCCTGCCCTTG 20970 TCCTTGCTCATCCTGCCCTTG

TABLE 26-25 Haruna Nijo Clones H602 SEQ ID NO: baal39g02 TTCTGGCCGGTGGTCTTGTCC 20971 TTCTGGCCGGAGGTCTTGTCC baal39g02 TTGTCGTTGGTGATGGTGATC 20972 TTGTCGTTGGAGATGGTGATC baal39g02 ACTTCTCTGCGTCCTGGACCA 20973 ACTTCTCTGCATCCTGGACCA baal39g02 TCATGTTGTAGGCGTAGTTCT 20974 TCATGTTGTAAGCGTAGTTCT baal39g02 GAGACGTTCAGGATACCGTTG 20975 GAGACGTTCAAGATACCGTTG bags22c13 TCCGTAGCTCGGGCAGGCCAA 20977 TCCGTAGCTCAGGCAGGCCAA bags22c13 TCTTATGGCAGAATAAAAATA 20978 TCTTATGGCAAAATAAAAATA bags22c13 GGCCAACCAATCTTTGAAGAT 20979 GGCCAACCAACCTTTGAAGAT baal16h03 GATCCTCTTCCGAGTTCTGCC 20981 GATCCTCTTCTGAGTTCTGCC bags18o20 AGATCTGGCCAAAGCCACCAC 20984 AGATCTGGCCGAAGCCACCAC bags18o20 CAACTCGTGATTCTGTCAGAA 20985 CAACTCGTGACTCTGTCAGAA bags19c02 TTATGCCACACCACACGAATA 20991 TTATGCCACATCACACGAATA bah52e20 AGNACCCTAAGCTGATCTTGA 20992 AGAACCCTAACCTGATCTTGA baal4n05 TGTCCTCAAA-TCAGATAGAG 20993 TGTCCTCAAAATCAGATAGAG 20994 bags7p06 GTCCTATCCAGCCAGATAAAA 20995 GTCCTATCCAACCAGATAAAA bast70c0905  AGATAGATTGCGGGGTTACAG 20996 AGATAGATTGGGGGGTTACAG basd15j01 TGTACAAAGTAGGGGAGATGA 20997 TGTACAAAGTGGGGGAGATGA basd15j01 TGACCGCCACGATGTACTTGG 20998 TGACCGCCACAATGTACTTGG BaH56H18 AACAGAATCAGAAGTTCAGAG 20999 AACAGAATCAAAAGTTCAGAG BaGS4N04 GTTTGCAGCCATAGTCCATTC 21000 GTTTGCAGCCGTAGTCCATTC bags31o13 TTGTGCAAAGGAAGGACAACT 21001 TTGTGCAAAGCAAGGACAACT bags31o13 ACTAGCGGGTTGCTACACCGG 21002 ACTAGCGGGTCGCTACACCGG bags31o13 GCTAACTATATCATACAAGAG 21003 GCTAACTATAGCATACAAGAG bags31o13 CCGACGTCGTCGGCGGGAGGA 21004 CCGACGTCGTTGGCGGGAGGA bags7h14 TTGTTCATTACTTAATTGTTA 21006 TTGTTCATTAGTTAATTGTTA

TABLE 26-26 Haruna Nijo Clones H602 SEQ ID NO: bags7h14 CCACGGCCGGAAGTGGCCGAT 21007 CCACGGCCGGCAGTGGCCGAT bags7h14 ACTGTGGGACGATGCCCTCCT 21008 ACTGTGGGACAATGCCCTCCT bags7h14 ATTGAGGGGGAAAACGATCCG 21009 ATTGAGGGGGGAAACGATCCG bags7h14 GTATGGCCGCGCCACTCGACG 21010 GTATGGCCGCCCCACTCGACG bags5n14 CACACTGGGGAAAGGCCTGGC 21011 CACACTGGGGGAAGGCCTGGC bags5n14 CCGTCACAAGCGTCGCAGACT 21012 CCGTCACAAGAGTCGCAGACT bastl32h0816 CGCACCCAGACGCGAGATGCC 21013 CGCACCCAGATGCGAGATGCC bastl32h0816 GTGCTGCCACTCAAGGCTAGC 21014 GTGCTGCCACCCAAGGCTAGC bastl32h0816 GCCAGCAAAGGTTAGATACAT 21015 GCCAGCAAAGATTAGATACAT baak31p06 AAACAGAGGCTGATTTTTTGT 21016 AAACAGAGGC-GATTTTTTGT 21017 baak31p06 TTGGTAAGACGTGCTGCTTAT 21018 TTGGTAAGACATGCTGCTTAT baak44j08 TGATGGTATTGTTGCTCCCCG 21021 TGATGGTATTATTGCTCCCCG baak44j08 TGTGGAAGCTTCCGGATACAT 21022 TGTGGAAGCTCCCGGATACAT baak18g16 CTGATATCCGGCTCAGCTCGT 21023 CTGATATCCGACTCAGCTCGT baak18g16 TGAACATCGGGGATGCCTGCT 21024 TGAACATCGGTGATGCCTGCT baak18g16 CGCGCCGCTAGACGCGAAGCA 21025 CGCGCCGCTACACGCGAAGCA baak18g16 CCTTGGAGCACCTTTGAAGCC 21026 CCTTGGAGCATCTTTGAAGCC baak11a11 GCCCACCAAGTAATTGTGAGC 21027 GCCCACCAAGCAATTGTGAGC baal9n02 ACTCCAGAGGGGCTCGCCCTT 21029 ACTCCAGAGGTGCTCGCCCTT baal9n02 GCGAGAAAGAAAAGGTGTTTC 21030 GCGAGAAAGACAAGGTGTTTC baal9n02 GCGGGCTCAGGGAACGCGTCC 21031 GCGGGCTCAGTGAACGCGTCC baal9n02 ATGAGCTACGTGCGGGCTGGC 21032 ATGAGCTACGGGCGGGCTGGC baal9n02 ACTCCGGGGAGACATGGAATA 21033 ACTCCGGGGAAACATGGAATA bags20f08 AGGAAACAGCGAGCTCAGGCT 21034 AGGAAACAGCAAGCTCAGGCT bags20f08 AATACTAAAACGACACAAAGA 21035 AATACTAAAATGACACAAAGA

TABLE 26-27 Haruna Nijo Clones H602 SEQ ID NO: bags20f08 GGAATATGCAATAACATATAC 21036 GGAATATGCAGTAACATATAC bags9c24 ACAGCAGTTGCATCAGTGGAT 21037 ACAGCAGTTGGATCAGTGGAT BaGS22O02 TGTCCTTCTCCGCCAGCACAG 21038 TGTCCTTCTCTGCCAGCACAG BaGS22O02 CCAGCACGCCGAAGAAGATGG 21039 CCAGCACGCCAAAGAAGATGG BaGS22O02 CGCCGGGACCTTCCTCTCTCA 21040 CGCCGGGACCATCCTCTCTCA bah41b17 AGGTCATGAATGTAATCACAG 21042 AGGTCATGAACGTAATCACAG bah41b17 TAGTCTCTGCAGGCCTGATGT 21043 TAGTCTCTGCGGGCCTGATGT bah63m11 AAATAGACGGCTGAAATTAAT 21044 AAATAGACGGGTGAAATTAAT bags28p07 TTTTTCATGCTCCATGCCCTT 21045 TTTTTCATGCACCATGCCCTT bags28p07 CTTTCAATCATACGACTGACT 21046 CTTTCAATCACACGACTGACT bast03d0408  AAGCAGATTCCTTCATTAGCC 21049 AAGCAGATTCTTTCATTAGCC baal17g06 CAAACTGGTTTCCGAAGAGCC 21050 CAAACTGGTTCCCGAAGAGCC baal17g06 AGACCCTTGCCACGCCGCCGG 21051 AGACCCTTGCGACGCCGCCGG baal17g06 ACAGTACAACTTATTATCCAC 21052 ACAGTACAACGTATTATCCAC baal17g06 ACACAAATACCTACTAGTACA 21053 ACACAAATACATACTAGTACA bags4p17 GCATCCGTTGTTGGAGTCGCA 21055 GCATCCGTTGCTGGAGTCGCA bags4p17 GGCGGCCGCCCCCGTACCAGC 21056 GGCGGCCGCCTCCGTACCAGC bags4p17 ATCATCTTCCAGCACCGGCGG 21057 ATCATCTTCCCGCACCGGCGG bags4p17 CTGCTCGCTGCTGATGCGGAT 21058 CTGCTCGCTGGTGATGCGGAT bags4p17 GTGTTGAGCCCGAGAGCCTTC 21059 GTGTTGAGCCTGAGAGCCTTC bags4p17 CGGGGACCTCGCCGATGTCGG 21060 CGGGGACCTCCCCGATGTCGG bags4p17 GTCCACGATGTTGTCCTTGCA 21061 GTCCACGATGCTGTCCTTGCA baal31o21 AGGGGTAGGAGAAAATGATGC 21062 AGGGGTAGGAAAAAATGATGC baal31o21 CCATGCCACAGGAGATAGACA 21063 CCATGCCACAAGAGATAGACA baal31o21 GGTGGTCCGCAGTGGCTGGCT 21064 GGTGGTCCGCGGTGGCTGGCT

TABLE 26-28 Haruna Nijo Clones H602 SEQ ID NO: bags4d11 TCTTCTTCCCAAATTTCAGCA 21068 TCTTCTTCCCGAATTTCAGCA basd13n18 AGATAATCAATAAACAAAGTG 21067 AGATAATCAACAAACAAAGTG basd0a08 AGACCCTTGCGACGCCGCCGG 21068 AGACCCTTGCCACGCCGCCGG basd0a08 ACAGTACAACGTATTATCCAC 21069 ACAGTACAACTTATTATCCAC basd0a08 CAAACTGGTTCCCGAAGAGCC 21070 CAAACTGGTTTCCGAAGAGCC basd0a08 ACACAAATACGTACTAGTACA 21071 ACACAAATACCTACTAGTACA bags32p24 CTTAAACATACGCATCATATG 21072 CTTAAACATATGCATCATATG kr06h0315 ACCANACTCTCCTGAGCAGAC 21074 ACCAGACTCTGCTGAGCAGAC baal26b09 GACCGAGACCGTGTCCGTGTC 21075 GACCGAGACCATGTCCGTGTC kr32c1105 TATACACATACGAGTAGGATT 21076 TATACACATAGGAGTAGGATT kr32c1105 TTGTGGTTGTCGTTGTAGGTA 21077 TTGTGGTTGTGGTTGTAGGTA kr32c1105 ACTTTCTTTCTTTCTTTCTTT 21078 ACTTTCTTTCCTTCTTTCTTT bah53b15 ATTGCCAGAGGCAGAAATGCA 21079 ATTGCCAGAGACAGAAATGCA bags13h04 CAAGCCCTGCGTCAACGACAA 21080 CAAGCCCTGCATCAACGACAA baal15i11 AACCACCCTGAAAGAAAACTC 21081 AACCACCCTGGAAGAAAACTO

TABLE 27-1 Haruna Nijo Clones H602 SEQ ID NO: BaH30E11 TTCTTGGCCTCGGATTCGGAC 21084 TTCTTGGCCTTGGATTCGGAC BaH30E11 GGAATAATCCCCCTCATAACA 21085 GGAATAATCCACCTCATAACA BaH59J05 ATTACAGTTTTTTCGTCTACG 21086 ATTACAGTTTCTTCGTCTACG BaH59J05 AAAACATGGGAACTACATATA 21087 AAAACATGGGCACTACATATA BaAL39O22 AGTTGCGGCAGCGGCAATATG 21088 AGTTGCGGCATCGGCAATATG BaAL39O22 AACAGGCGTTTTCTGGTTAGT 21089 AACAGGCGTTGTCTGGTTAGT BaAL39O22  ATTCCAGACAAACATTTTTAT 21090 ATTCCAGACACACATTTTTAT BaAL39O22 GCCGGGGAATACAACCATCAG 21091 GCCGGGGAATGCAACCATCAG baal16i24 ATCCATCATGGCAGCTCATAC 21092 ATCCATCATGCCAGCTCATAC baak43j24 GCCAGTTGATCCCTGCCAACA 21097 GCCAGTTGATGCCTGCCAACA baak43j24 CCACACTGAAATTCATACCTC 21098 CCACACTGAATTTCATACCTC bags3m02 GCGCAGCACCACCGGACCCGA 21109 GCGCAGCACCGCCGGACCCGA bags3m02 CTTCTCTCTCTTCCCTTCCTT 21110 CTTCTCTCTCCTCCCTTCCTT baal36l08 ACCTACCACCAATCGCAAAGC 21111 ACCTACCACCCATCGCAAAGC baal36l08 CAGCTCTTCGCCATCAAGCAC 21112 CAGCTCTTCGGCATCAAGCAC baet19e0309 TTACAGTGACCCGGGCGCCGG 21113 TTACAGTGACACGGGCGCCGG bast48d0307 CCATGTAGGCGCCCGGTCTCC 21114 CCATGTAGGCACCCGGTCTCC kr55D0707 GGTTGACCGGCGCTGGTGGAA 21115 GGTTGACCGGTGCTGGTGGAA kr55D0707 CTGCCAGCGCCGACGCCACGG 21116 CTGCCAGCGCAGACGCCACGG BaGS24F04 TAACTAACCACAAGACTGCTC 21119 TAACTAACCAAAAGACTGCTC BaGS24F04 GGCGACTTGGTCTCCTTCTCG 21120 GGCGACTTGGCCTCCTTCTCG bags26p07 TCTCCCGGGAGGTGATGGTGG 21122 TCTCCCGGGACGTGATGGTGG bags26p07 CGTGCTTGGCGAGCTCGCCGG 21123 CGTGCTTGGCCAGCTCGCCGG bags12f11 TGGCCTTCCCGTCCGAGCGGT 21124 TGGCCTTCCCATCCGAGCGGT bags20o14 CTAAAGAACAGAATTATTCAC 21127 CTAAAGAACAAAATTATTCAC

TABLE 27-2 Haruna Nijo Clones H602 SEQ ID NO: bags20o14 CATGGAAAGAGATATGCATAT 21128 CATGGAAAGAAATATGCATAT bags20o14 AAATGGGGTTGTTAATGACGG 21129 AAATGGGGTTCTTAATGACGG bags20o14 AAAACTATCATGGGCACTGAC 21130 AAAACTATCAGGGGCACTGAC bags20o14 GCACTGACCAGCTTGCTTGAC 21131 GCACTGACCATCTTGCTTGAC bags20o14 TCTGTGGAAGCTGGTCTTCAC 21132 TCTGTGGAAGTTGGTCTTCAC baak36d08 GGAAGACGGCAGGGTGGAATG 21133 GGAAGACGGCGGGGTGGAATG baak36d08 TGGCGGTTGGAGGCAACCAGT 21134 TGGCGGTTGGGGGCAACCAGT bast75h0616 ATATATTTTCCAAGCAAGAGG  21135 ATATATTTTCAAAGCAAGAGG bast75h0616 TGGCGGTTGGGGGCAACCAGT 21136 TGGCGGTTGGAGGCAACCAGT basd16g17 CTAGCCGCCTTGCAGCCCCAC 21137 CTAGCCGCCTCGCAGCCCCAC BaH21K13 AACAAATTTCCGTACACAACA 21143 AACAAATTTCTGTACACAACA BaH21K13 AAGAGAGGAGACAACAAATTT 21144 AAGAGAGGAGGCAACAAATTT baak14j02 ACAGCCTTACATCGGGGAACT 21145 ACAGCCTTACGTCGGGGAACT baak14j02 TGAAGCCGACGGTTCTGTCCA 21146 TGAAGCCGACCGTTCTGTCCA bah22c08 CCACTCCCATGGTTAAATGTA 21147 CCACTCCCATAGTTAAATGTA bah22c08 TTCTGTGTTAAAGCTTCTTAC 21148 TTCTGTGTTACAGCTTCTTAC bah22c08 AAATGTAATACGAAATAATGA 21149 AAATGTAATATGAAATAATGA bah22c08 CCACATACCCTATCCAGAACA 21150 CCACATACCCGATCCAGAACA bah22c08 CTTATAAAAGGAAAACTCATA 21151 CTTATAAAAGAAAAACTCATA bah22c08 TCACCTTGATCTCAAGAATGT 21152 TCACCTTGATTTCAAGAATGT bah22c08 ATATCATTCGTGGGCACAAAA 21153 ATATCATTCGCGGGCACAAAA bah12n12 TGGGCGTCTTGAAGTACAAAT 21157 TGGGCGTCTTAAAGTACAAAT baak32l04 ATTCATAACAGGAGCATCCTT 21161 ATTCATAACAAGAGCATCCTT baak32l04 CATGCTGGGC-GGGAAGGCCC 21162 CATGCTGGGCAGGGAAGGCCC 21163 baak32l04 CCCTCCGCGTCGCCACCGGGG 21164 CCCTCCGCGTTGCCACCGGGG

TABLE 27-3 Haruna Nijo Clones H602 SEQ ID NO: baak32l04 TCTGCCCTTCGCCGTAGCCAA 21165 TCTGCCCTTCTCCGTAGCCAA BaAL7C19 GTACCTTGGATATGGTGAGGG 21167 GTACCTTGGAGATGGTGAGGG BaAL7C19 GGAGTAGTAGATAGACAAGAT 21168 GGAGTAGTAGGTAGACAAGAT BaAK23F04 CTCCATCGCTTGCTTCGAGCC 21169 CTCCATCGCTCGCTTCGAGCC basd0g08 ATATATCTCCGAGCACCCCTT 21171 ATATATCTCCAAGCACCCCTT basd0g08 GAACTGTACAGTACTCCTGGT 21172 GAACTGTACATTACTCCTGGT basd0g08 GTTGCCCGTCCCAGGAGAAGC 21173 GTTGCCCGTCTCAGGAGAAGC basd0g08 TCAATATGGATTTGCCCTGTG 21174 TCAATATGGACTTGCCCTGTG baal21b21 CGACGACGACTACGACGGGCG 21176 CGACGACGACGACGACGGGCG baal21b21 GACCGACGACGCGACAGGGGA 21177 GACCGACGACACGACAGGGGA baal21b21 CTTGGAGCAGGTGCCGCTGCG 21178 CTTGGAGCAGATGCCGCTGCG bags30e05 ACTGTGTACTACATTGTTTCT 21179 ACTGTGTACTGCATTGTTTCT bags30e05  CTTGCACACTGCAAAGAACTG 21180 CTTGCACACTACAAAGAACTG bags30e05 AGCCATTACTGTAAACTCACC 21181 AGCCATTACTTTAAACTCACC bags30e05 GTCGAACAAGACTGGGCCGTC 21182 GTCGAACAAGGCTGGGCCGTC bags30e05 ACTTCCCTCCGGTAAGATAAA 21183 ACTTCCCTCCCGTAAGATAAA bags30e05 CATGGATGAGAACTTGCACAC 21184 CATGGATGAGGACTTGCACAC bals30e05 GGCAAATGTGTCTGACAGCCA 21185 GGCAAATGTGCCTGACAGCCA baak13g04 CACTGACACCGTCCGATCCAG 21186 CACTGACACCCTCCGATCCAG bags9a04 GCCCTTCCTTGTGGCCCATCC 21187 GCCCTTCCTTATGGCCCATCC bags9a04 AGTTCCTGTTCGTCGTTGATA 21188 AGTTCCTGTTTGTCGTTGATA bags9a04 TCCCCAATCATAAGGATGTGG 21189 TCCCCAATCAGAAGGATGTGG bags9a04 TCGCTCCTCTGAACTAGCAAC 21190 TCGCTCCTCTAAACTAGCAAC bags9a04 CTGCTAAGCATCAACAAATAA 21191 CTGCTAAGCAGCAACAAATAA baak23m11 GGCGAGCACTCGTCCTCTTCA 21193 GGCGAGCACTAGTCCTCTTCA

TABLE 27-4 Haruna Nijo Clones H602 SEQ ID NO: baak41p21 TGGAGGAGACCGTGTCAGCAA 21194 TGGAGGAGACGGTGTCAGCAA baak41p21 TGTAGATCTCATCAATAAGAG 21195 TGTAGATCTCGTCAATAAGAG baak26j14 GGAGTCCCCGACGCAATCTTT 21199 GGAGTCCCCGCCGCAATCTTT baak26j14 GTCACCTGTCGGTCACTCTGT 21200 GTCACCTGTCAGTCACTCTGT BaAK37E04 CGAAGAATGAAGGGGGGAAAA 21204 CGAAGAATGATGGGGGGAAAA BaAK37E04 GTGTTCATCTTAAAACAACAC 21205 GTGTTCATCTAAAAACAACAC BaAK37E04 CCTTCCTATGACTTCTCGGAT 21206 CCTTCCTATGGCTTCTCGGAT bastl31A0901 TAGAAACCAACTGGCGAGTTT 21210 TAGAAACCAATTGGCGAGTTT bah48o03 CGCAGCAGTCTGCTATCGCGC 21211 CGCAGCAGTCGGCTATCGCGC bah48o03 TCGCGCCGTAAGCCTCGGTGC 21212 TCGCGCCGTAGGCCTCGGTGC bah48o03 GATCAGTAAGCAGGAAGAAGC 21213 GATCAGTAAGTAGGAAGAAGC bah20k22 TATAGGGAACACATTTTCCGT 21214 TATAGGGAACGCATTTTCCGT bah47a17 TGCCTCACCATGTGACCAGCC 21217 TGCCTCACCAGGTGACCAGCC BaH56C11 TGTCATCCTTTTCATCCTCCT 21218 TGTCATCCTTCTCATCCTCCT basd15e10 TGAGCACAACCTGGCATGCCC 21219 TGAGCACAACTTGGCATGCCC BaH18C17 GAAGTGTTGTCAACTTCAAAG 21220 GAAGTGTTGTAAACTTCAAAG BaH18C17 TTACACCTTTTACCGTCTGAG 21221 TTACACCTTTCACCGTCTGAG BaH18C17 CAGCATCTCCTGATGGACAGA 21222 CAGCATCTCCAGATGGACAGA BaGS20G24 AAGAATCAGTATATTAGGTGG 21223 AAGAATCAGTCTATTAGGTGG BaGS20G24 ATAAAGACCTGGGAAACAAAG 21224 ATAAAGACCTAGGAAACAAAG bah52h21 AGCCTCGGGCGTTGCACATGC 21227 AGCCTCGGGCATTGCACATGC bah52h21 GGAGGGCCTACCCCGGCCGTG 21228 GGAGGGCCTATCCCGGCCGTG bah52h21 AACCACGCTGGCGCTGGAGGG 21229 AACCACGCTGACGCTGGAGGG bah52h21 GACGCCACACTATACTGTGTG 21230 GACGCCACACGATACTGTGTG bah52h21 GCGGGGGCGGATAGCTGGACC 21231 GCGGGGGCGGGTAGCTGGACC

TABLE 27-5 Haruna Nijo Clones H602 SEQ ID NO: basd18c12 ACATCTGGGGGAGCCAAACAG 21233 ACATCTGGGGCAGCCAAACAG basd18c12 TCGGCGTTTCATTCGCGCTAG 21234 TCGGCGTTTCGTTCGCGCTAG baak23k07 CAGCTTGACGGCATCAGGCAG 21235 CAGCTTGACGACATCAGGCAG baak23k07 AAAAATACACCAGCAGATCTG 21236 AAAAATACACAAGCAGATCTG BaSD15O17 ACATCTGACGAGAACAACACA 21237 ACATCTGACGGGAACAACACA bags16j16 TAGTACTCTATAGGATTTGAG 21238 TAGTACTCTACAGGATTTGAG bags16j16 ATTAGCAGGGTTATCAAATTG 21239 ATTAGCAGGGCTATCAAATTG bags16j16 ATAAAGAGAAATTCCTCCAGA 21240 ATAAAGAGAATTTCCTCCAGA BaGS4N23 TCGGCAGGTCGTAGTTGATGA 21243 TCGGCAGGTCATAGTTGATGA basd13i08 ACTCACTACTGCTGCTCTGCG 21246 ACTCACTACTGCTGCTCTGCG basd17p01 ACTCACTACTGCTGCTCTGCG 21247 ACTCACTACTACTGCTCTGCG baak13d19 TTACAAGTCAGAGAGAAAATG 21248 TTACAAGTCAAAGAGAAAATG baak13d19 ATAGTGTTAAGCATTAACACA 21249 ATAGTGTTAAACATTAACACA baal3h14 CACACAAAAGGGGAACCTAGG 21250 CACACAAAAGAGGAACCTAGG baal3h14 ATGCAACTAAGATATTGTCAG 21251 ATGCAACTAAAATATTGTCAG bags33j18 GCGGTTGCAATGCTCCACGTG 21252 GCGGTTGCAACGCTCCACGTG baak21n07 AGCACCTACACTGATCAGTGT 21254 AGCACCTACATTGATCAGTGT bah53f05 CATGTATCCAGTTCCATGCTG 21256 CATGTATCCACTTCCATGCTG bah53f05 CAAACTATTAGCAAACCTGCA 21257 CAAACTATTACCAAACCTGCA baak12d02 CATCGTTCGAGGCAATGACAC 21258 CATCGTTCGACGCAATGACAC baet01F1212 CGAACCCGAGCGGGTTGAAGA 21259 CGAACCCGAGTGGGTTGAAGA bags11h12 TATGATAGCAAATTGCTTGTA 21260 TATGATAGCATATTGCTTGTA bags11h12 GCAACTAGTCTTCTTTATGAT 21261 GCAACTAGTCCTCTTTATGAT bags11h12 AATGCTCAGAATTTCCGTAAG 21262 AATGCTCAGATTTTCCGTAAG bags11h12 GGCTAGGCCTTGAAGCGCTTG 21263 GGCTAGGCCTCGAAGCGCTTG

TABLE 27-6 Haruna Nijo Clones H602 SEQ ID NO: baak17l11 AATGCCACGGTTACTCATTGA 21264 AATGCCACGGATACTCATTGA baak17l11 GCCTGGAAAACGCAGTTTTCC 21265 GCCTGGAAAAGGCAGTTTTCC basd20e17 GGAGCCAGCGACCGGGAGAGC 21266 GGAGCCAGCGGCCGGGAGAGC bags30o05 TTGGAGGAACCTGCGGGTCGA 21267 TTGGAGGAACTTGCGGGTCGA bags30o05 AAAGATCGCACGCCACACCAT 21268 AAAGATCGCATGCCACACCAT bah29p24 TCTCATACCCTGAACCGTCTT 21269 TCTCATACCCCGAACCGTCTT kr68d0208 ATCACTAGTAATGTACATGTT 21270 ATCACTAGTAGTGTACATGTT bags37l11 CAGCCATCAACGCAACAAGTG 21272 CAGCCATCAATGCAACAAGTG bags37l11 TCATACAGTAGTGTTTTCAAG  21273 TCATACAGTAATGTTTTCAAG bags37l11 AACTTCATATGATCGCAAGGT 21274 AACTTCATATAATCGCAAGGT BaH58I23 AAAGCAAAGCATTCTTTGAAG  21275 AAAGCAAAGCGTTCTTTGAAG bags39l04 GGTCATCCTCGGGGTCTGTCC 21276 GGTCATCCTCAGGGTCTGTCC bags39l04 TTCCTCACAATCAGCGCTATG 21277 TTCCTCACAAACAGCGCTATG bags39l04 CTTTCCGCTGCAAGGCGGACA 21278 CTTTCCGCTGAAAGGCGGACA bags39l04 ATTGAACAGATCGAGTAGAGT 21279 ATTGAACAGACCGAGTAGAGT bah60p09 TACACACCAGTGGTCTGAAGA 21283 TACACACCAGCGGTCTGAAGA bah60p09 GTTGCCAGAAGATCACAATAA 21284 GTTGCCAGAAAATCACAATAA bah60p09 TACCTAGGGGCAGCTTGTTGA 21285 TACCTAGGGGTAGCTTGTTGA bah22o14 AGAAGTCTGCGGCAAACCTCA 21289 AGAAGTCTGCAGCAAACCTCA bah22o14 GATATTCTTGCGGTTATCTTC 21290 GATATTCTTGTGGTTATCTTC bah22o14 TCACGCTGTTTAGGGTAGGCT 21291 TCACGCTGTTCAGGGTAGGCT bah14l20 TATATAGTACNCCCTCTGTTC  21292 TATATAGTACTCCCTCTGTTC bah42p22 TCCGAAGTGGTTGGCCTTTCA 21293 TCCGAAGTGGCTGGCCTTTCA bah42p22 CTTATGCCCAGATTAAAATAC 21294 CTTATGCCCACATTAAAATAC baak21g03 CACGTACCCCGTCCTCTTGCT 21295 CACGTACCCCATCCTCTTGCT

TABLE 27-7 Haruna Nijo Clones H602 SEQ ID NO: baak21g03 GAATTTTTCAGGTTATATATG 21296 GAATTTTTCAAGTTATATATG baak21g03 GCGTGCAGATCATTGAATGTG 21297 GCGTGCAGATAATTGAATGTG baak21g03 ATTTAGCACACGATGAAGGGT 21298 ATTTAGCACATGATGAAGGGT baal35d24 CTCTCCCGCTGGATAGCGCGC 21299 CTCTCCCGCTAGATAGCGCGC baal35d24 AAGAGAGCTGTGGTCATCACG 21300 AAGAGAGCTGCGGTCATCACG baak45h14 GGGGACAAGTGGGGTGAGTTT 21301 GGGGACAAGTCGGGTGAGTTT baak45h14 GGACCCCTGGGTGGGGCTTTG 21302 GGACCCCTGGCTGGGGCTTTG baak45h14 GCTTTGGGGGCTCCCCTTTCC 21303 GCTTTGGGGGGTCCCCTTTCC baak45h14 TTTTTTTTGTCCAAAGGATTT 21304 TTTTTTTTGTACAAAGGATTT BaAK31P07 AAACATGACACACCATATATG 21305 AAACATGACATACCATATATG BaAK31P07 AAAAATATACTATCACAAATC 21306 AAAAATATACAATCACAAATC BaAK31P07 AATCCACAGGTTTTATCTTCG 21307 AATCCACAGGGTTTATCTTCG bags28o05 CTTTGTGCACGAGGGCATTAA 21308 CTTTGTGCACAAGGGCATTAA bah36c06 CAAGACGCCCGGTCCGGCCAG 21309 CAAGACGCCCAGTCCGGCCAG bags37h24 AACTGTCATCGTCAGTCTCAT 21310 AACTGTCATCATCAGTCTCAT bast65b0303 AGCAGCAGCAGCAACAACAAC 21311 AGCAGCAGCAACAACAACAAC BaGS39K23 ATCTGCGCGCCCGGCCTTTTG 21312 ATCTGCGCGCGCGGCCTTTTG BaGS39K23 AAGAACTGACAGAGGACACGA 21313 AAGAACTGACGGAGGACACGA bah51a22 TACCCAGTTGCTTCGATGTTT 21314 TACCCAGTTGGTTCGATGTTT BaH57F12 CTGCAGGTTGACGGTGCTGGT 21315 CTGCAGGTTGTCGGTGCTGGT BaH57F12 TCATCTGAAGGTTCAGTTCCG 21316 TCATCTGAAGCTTCAGTTCCG BaH57F12 ATTGTGTGGCGAAACCATCCG 21317 ATTGTGTGGCAAAACCATCCG basd14o12 TTATCTACATACCTAGATTTG 21318 TTATCTACATCCCTAGATTTG basd14o12 CACCTTTGCATTGGCTCACCA 21319 CACCTTTGCAGTGGCTCACCA BaAL40N06 ATCTCCAAACGAACAGCCTCT 21321 ATCTCCAAACAAACAGCCTCT

TABLE 27-8 Haruna Nijo Clones H602 SEQ ID NO: BaAL40N06 ACTTGTTTGACAGATTAACTA 21322 ACTTGTTTGATAGATTAACTA bah11b14 TTAAAACTAGATAACTGAAAT 21323 TTAAAACTAGGTAACTGAAAT bah15j14 AGCACTCCTG-ACTAAAAAGT 21324 AGCACTCCTGCACTAAAAAGT 21325 basd21l07 CAGCAGCTGGCTGTACACCTC 21330 CAGCAGCTGGTTGTACACCTC bah42d07 AGAGGTGAAGGACACCGGACG 21331 AGAGGTGAAGAACACCGGACG bah42d07 AGCTACCACAGAAGTCCTTTC 21332 AGCTACCACAAAAGTCCTTTC bah42d07 CCCATGTCTGGACAACAAAAG 21333 CCCATGTCTGCACAACAAAAG BaAK24L01 CTTGCTTAGAGGGACCGATGT 21334 CTTGCTTAGATGGACCGATGT BaAK24L01 ATCGAGCTGTGGGCGACGAGG 21335 ATCGAGCTGTAGGCGACGAGG bags23k24 TCTTGATGTCGGTCAGTGAAA 21336 TCTTGATGTCAGTCAGTGAAA BaAL11H22 TGATGACATCATGCTCCTCTC 21337 TGATGACATCGTGCTCCTCTC baal6m22 CAGATGTACACATGGAATGGA 21339 CAGATGTACATATGGAATGGA basd20p03 CCCATTTACGAGGGAAGTTCA 21340 CCCATTTACGGGGGAAGTTCA kr59H0416 CACATGACATAAAATCGCTAA 21341 CACATGACATGAAATCGCTAA bah19b13 TACAACATCCGAAGCAGCCAC 21342 TACAACATCCAAGGCAGCCAC bah19b13 CAACATCCGAAGCAGCCACAT 21343 CAACATCCAAGGCAGCCACAT BaGS14A02 AATGTAAACCGCAGGCTGCTT 21344 AATGTAAACCACAGGCTGCTT bah61o16 TATAAGTCCCGTGACACTGCA 21345 TATAAGTCCCATGACACTGCA bah34p05 TAATGCATCAGCTACACACTT 21346 TAATGCATCAACTACACACTT bah24n07 CCACGAGCACGAAAAGTAGCG 21348 CCACGAGCACAAAAAGTAGCG bah54b04 TGCGGCTTCTGCGCCTCCTCA 21349 TGCGGCTTCTACGCCTCCTCA bah54b04 GCTTGCTATTCCGAAGTCACT 21350 GCTTGCTATTACGAAGTCACT bah54b04 AATAATGACTGCAGACAAAAT 21351 AATAATGACTGCAGACAAAAT baal11h20 ATGACTCTGGTCCATATTTGT 21352 ATGACTCTGGCCCATATTTGT basd20m23 CGTATGTAGATATCTAGTGAA 21354 CGTATGTAGACATCTAGTGAA

TABLE 27-9 Haruna Nijo Clones H602 SEQ ID NO: basd20m23 AGTATAGATTTATTCATTTTG 21355 AGTATAGATTCATTCATTTTG basd20m23 ATATAAGTCTCTCTAGAGGTT 21356 ATATAAGTCTTTCTAGAGGTT basd20m23 AGGACCTGCTCTTGTTGAACG 21357 AGGACCTGCTTTTGTTGAACG baal15d09 GGTTGATCTCCGCTTGGCTTC 21358 GGTTGATCTCTGCTTGGCTTC baak39n20 TGACATCCTCGAACCCTGCCT 21359 TGACATCCTCAAACCCTGCCT bah43n16 AACTCCTCTTCCTCGGATACG 21361 AACTCCTCTTTCTCGGATACG basd22c03 TGTATGTAAGCAGCTTGTTCC 21363 TGTATGTAAGTAGCTTGTTCC kr47F0511 CATAACATCAATGATAAACGA 21364 CATAACATCAGTGATAAACGA kr47F0511 AGCTTCTTGTCCCCTCCTCCT 21365 AGCTTCTTGTTCCCTCCTCCT bast77a0402 ATCATCTAACCCATATAACCA 21366 ATCATCTAACGCATATAACCA bah58f04 CACTCTCCTCGAGATTAAATA 21367 CACTCTCCTCAAGATTAAATA kr39G1113 GTGTTTTTGGATATGATCTGA 21368 GTGTTTTTGGGTATGATCTGA baak30l09 TGCCCATCATGATTCATCAGA 21369 TGCCCATCATCATTCATCAGA baak1c16 TCCGCAAAAAAACACACTATA 21370 TCCGCAAAAACACACACTATA BaAK12F18  AATTCTTAGCCATGAATACAA 21371 AATTCTTAGCAATGAATACAA BaAK12F18 CCTTGGGGTCAATGCCGTCGA 21372 CCTTGGGGTCCATGCCGTCGA BaAK12F18 GGTGCAGCCAAGCTGACGCAG 21373 GGTGCAGCCACGCTGACGCAG BaAK12F18 TCTTAAGCGGAGATCTCAACC 21374 TCTTAAGCGGGGATCTCAACC BaAK12F18 TATAAGTCACACCATCCCTAA 21375 TATAAGTCACGCCATCCCTAA BaAK12F18 GGGCTAGCATGAGCATACAAT 21376 GGGCTAGCATAAGCATACAAT basd20e05 ATTCAGAGAGGGAGATGCAGT 21377 ATTCAGAGAGAGAGATGCAGT basd20e05 AGCAGAAATATCATCAACTGG 21378 AGCAGAAATACCATCAACTGG basd20e05 TTAGCGTCATCGTGTTCCCTT 21379 TTAGCGTCATAGTGTTCCCTT baal17d05 TATCACAACTCGATATTCCAT 21382 TATCACAACTGGATATTCCAT baal17d05 CATGTATACTCTCTGCATGCA 21383 CATGTATACTTTCTGCATGCA

TABLE 27-10 Haruna Nijo Clones H602 SEQ ID NO: baal17d05 AAGACTGACTCACTTAGAAAA 21384 AAGACTGACTGACTTAGAAAA bast70d0107 TAATAATGTTCAACTTCAATA 21385 TAATAATGTTGAACTTCAATA baak46g03 TGGCAAACACAGATTTATAAC 21386 TGGCAAACACGGATTTATAAC bah63m17 TGAGCAGTTACACTAAAGAGA 21387 TGAGCAGTTATACTAAAGAGA bah63m17 TCTCACGTGGACTGAGATAAC 21388 TCTCACGTGGGCTGAGATAAC bah15l15 AGACAACCACGTGAGAGCAGC 21389 AGACAACCACATGAGAGCAGC bah15l15 TGATCCCGTTCGCGTCGAGGT 21390 TGATCCCGTTGGCGTCGAGGT bags19n01 GCAAAATTCACGATTTTAAGA 21391 GCAAAATTCAGGATTTTAAGA bags19n01 AAGAGAAAAACATTACAAGCT  21392 AAGAGAAAAAGATTACAAGCT bags19n01 CGTAGTACAAAAGTATCAAGA 21393 CGTAGTACAACAGTATCAAGA baak32p07 AACAATGACAGCTTTGGCTGG 21394 AACAATGACACCTTTGGCTGG baak32p07 CAGAAAATGGCTTTATTGATT 21395 CAGAAAATGGATTTATTGATT bags14l13 AAATGAGTAGCGACATGCCAG 21396 AAATGAGTAGGGACATGCCAG bags14l13 TAAGAAGGCAAATAACTGGTA 21397 TAAGAAGGCAGATAACTGGTA bags14l13 TACCCGCGGTTGCAAGCTCCT 21398 TACCCGCGGTCGCAAGCTCCT bags14l13 GACAGAAAGATGTTCATGGTT 21399 GACAGAAAGACGTTCATGGTT bags37o24 ACACAAAACAGAGCAAATGTG 21400 ACACAAAACAAAGCAAATGTG bags37o24 TTAAGTTCTAGCTGATTGAAA  21401 TTAAGTTCTATCTGATTGAAA bags37o24 CGGACATGTAGCTGTATCAAC  21402 CGGACATGTAACTGTATCAAC baal27m03 AAACAGGGGANAGGGACAACC 21406 AAACAGGGGAGAGGGACAACC baal27m03 CTTTCACCGTCTGAGGGCATG 21407 CTTTCACCGTTTGAGGGCATG baal27m03 GCTCAGCTGCNAGGAAAATAA 21408 GCTCAGCTGCAAGGAAAATAA baal27m03 GGGCATCAGCNGATCGAGCTA 21409 GGGCATCAGCCGATCGAGCTA baal27m03 TCATATCACTGATTGGGGAAC 21410 TCATATCACTTATTGGGGAAC BaAK1C18 ACTATGCACTGGTATATAACT  21411 ACTATGCACTAGTATATAACT

TABLE 27-11 Haruna Nijo Clones H602 SEQ ID NO: BaAK1C18 GCGACCCTTGTTGGGGGCAAC 21412 GCGACCCTTGCTGGGGGCAAC bah52b11 TCTATGGAAA-TACATGGAAG 21416 TCTATGGAAAATACATGGAAG 21417 bah27f05 CGAGATGTACGCTCTGCCGAC 21419 CGAGATGTACCCTCTGCCGAC bah59c10 ACGAGAAAACTAAATGAAATC 21420 ACGAGAAAACCAAATGAAATC basd14e01 CCTCGCCGTAGGCCGCGATGT 21421 CCTCGCCGTAAGCCGCGATGT basd14e01 TGATGTCGGCCGTCTCGTGCT 21422 TGATGTCGGCTGTCTCGTGCT basd14e01 CACACGGCGATCGGAGCTTCA 21423 CACACGGCGACCGGAGCTTCA bags38j06 GTTTCCTCTACTGTAACGGAT 21424 GTTTCCTCTAGTGTAACGGAT bags38j06 AATAGATTGTGTGAATTTGTG 21425 AATAGATTGTTTGAATTTGTG basd18e06 CCTCTTTATTCTCATCTCCTC 21429 CCTCTTTATTTTCATCTCCTC basd18e06 TGGTGTTGACAGCGAGCTTGA 21430 TGGTGTTGACGGCGAGCTTGA basd18e06 AGTAGAGCCGTCGGAACTCAA 21431 AGTAGAGCCGGCGGAACTCAA baak16e08 AAGGGGAAGGTTCACGTGCAG 21432 AAGGGGAAGGCTCACGTGCAG basd2o16 TCAAAACAAACAAGCTCCGTC 21433 TCAAAACAAAGAAGCTCCGTC basd2o16 GGACAGGGTAGAAGGGTAGAG 21434 GGACAGGGTACAAGGGTAGAG basd2o16 TTGAAGGGGATCTGCTTCTTG 21435 TTGAAGGGGAACTGCTTCTTG basd2o16 CTTGAAACGGATCCGGAACAG 21436 CTTGAAACGGTTCCGGAACAG bags18h01 GAAACAGATTGTTAAAAGATT 21437 GAAACAGATTTTTAAAAGATT bags18h01 CGATTTAGTATTTCCCCTGGT 21438 CGATTTAGTAATTCCCCTGGT bags18h01 ACACACAAAACAGAAAACAAC 21439 ACACACAAAAAAGAAAACAAC bags4l20 GGAAATTTTACCTGCCTCGAT 21441 GGAAATTTTATCTGCCTCGAT bah30j08 TTATTGTCTGTTTGTCACATG 21450 TTATTGTCTGCTTGTCACATG bah30j08 GGATTTCTGTCCTCATGAAAG 21451 GGATTTCTGTGCTCATGAAAG bah30j08 AGGGAACATTGGAATCAGCCA 21452 AGGGAACATTAGAATCAGCCA bah30j08 TGAATTAACCATACGGCTAAT 21453 TGAATTAACCGTACGGCTAAT

TABLE 27-12 Haruna Niio SEQ Clones H602 ID NO.: bah30j08 GCATTCTCGGTCACCGCCTTG 21454 GCATTCTCGGGCACCGCCTTG bah30j08 GCTTCGCTGCGTCTGAATTAA 21455 GCTTCGCTGCCTCTGAATTAA bast22G0313 TTCTAGTTACAGGTCTTCAGC 21456 TTCTAGTTACGGGTCTTCAGC bags33n21 CACCTGCGCTCTAACATCCTT 21458 CACCTGCGCTGTAACATCCTT bags33n21 AAACAAGTAATTGATACAGTC 21459 AAACAAGTAACTGATACAGTC bags33n21 CAAACTTTCCAGGGGGGCAAA 21460 CAAACTTTCCCGGGGGGCAAA baak33c11 ATCTATTCCTTCAAATTTTGA 21461 ATCTATTCCTCCAAATTTTGA baak33c11 TCCATCACTCGGAGCATATTC 21462 TCCATCACTCCGAGCATATTC BaH36P06 CATCTCCGGCAGTCTCAACAG 21464 CATCTCCGGCGGTCTCAACAG BaH36P06 GATATGAATCGGGAGTTGTTG 21465 GATATGAATCAGGAGTTGTTG BaH36P06 TGTTTCTTTTCGATTAAATCA 21466 TGTTTCTTTTTGATTAAATCA BaH36P06 GTACCATCCGGGCAAGAATCG 21467 GTACCATCCGAGCAAGAATCG baet25H0115 TCTCAGCGGCTCCATGGTGTG 21468 TCTCAGCGGCCCCATGGTGTG bald12l01 CGCTTCACAGACGCAAAACAC 21469 CGCTTCACAGGCGCAAAACAC basd12l0l TGCCCGACGCTTCCAAGATCT 21470 TGCCCGACGCATCCAAGATCT BaAK29I15 CCGTGCCGACGTGTGCTTGCT 21474 CCGTGCCGACATGTGCTTGCT BaAK29I15 TCCGGTGTGTCCGTGCTGCCC 21475 TCCGGTGTGTACGTGCTGCCC bast62B0404 GGCGGGTACAGACAAGCAACC 21476 GGCGGGTACATACAAGCAACC bast62B0404 ATCTCCATCTTATTATTCTGC 21477 ATCTCCATCTCATTATTCTGC bast62B0404 AAGTTTCAAGTAGCTGCCGCT 21478 AAGTTTCAAGCAGCTGCCGCT baak18i01 AAGAAATGATACCCAAATTGA 21479 AAGAAATGATCCCCAAATTGA baak18i01 AATGATTAGGCGGCTATCAGG 21480 AATGATTAGGGGGCTATCAGG bah25l03 CTGCGAGCTCAACCGATGATT 21482 CTGCGAGCTCGACCGATGATT bah25l03 TGTGAGCCTCGCTGCCTTTAC 21483 TGTGAGCCTCACTGCCTTTAC bah25l03 AAACCAAAAGAAGCGGCACAC 21484 AAACCAAAAGCAGCGGCACAC

TABLE 27-13 Haruna Niio SEQ Clones H602 ID NO: bah25l03 TTGATCAATAGTAGAAACTCA 21485 TTGATCAATACTAGAAACTCA bah25l03 AACATGTATATGAAAAGATCA 21486 AACATGTATACGAAAAGATCA bah25l03 ATGATTACAAGAAGATCGGAG 21487 ATGATTACAAAAAGATCGGAG bah25l03 TCGCATGATCGTTAAGAATGA 21488 TCGCATGATCATTAAGAATGA baal1n11 GCAAGCCCTTGCAATACCGTA 21489 GCAAGCCCTTTCAATACCGTA baak16l07 TTCCTTATGGGCAGTGAGCAG 21490 TACGTTATGGACAGTGAGCAG baal13c18 TTACTTTGCATCGTTGATGAC 21494 TTACTTTGCACCGTTGATGAC baal13c18 GAGCGCTGGTGTTGTAGACGA 21495 GAGCGCTGGTATTGTAGACGA baal13c18 GAACAAATGACAGAAATTTTC 21496 GAACAAATGAAAGAAATTTTC baal13c18 CATGCACATTGTAGCTACGTA 21497 CATGCACATTATAGCTACGTA baal13c18 TGACGGCCCATATAAGAAGCC 21498 TGACGGCCCAGATAAGAAGCC baal13c18 TGAGTCCACTGTCAGTGAAAA 21499 TGAGTCCACTTTCAGTGAAAA baak29k23 ACCTCCTTTTGAAATTCATAT 21500 ACCTCCTTTTCAAATTCATAT baak29k23 GAGCGCTGGTGTTGTAGACGA 21501 GAGCGCTGGTATTGTAGACGA baak29k23 CATGCACATTGTAGCTACGTA 21502 CATGCACATTATAGCTACGTA bags7d17 CATGGTGTCAGCTTTAATATA 21503 CATGGTGTCAGCTTTAATATA bags7d17 GCAAGCTTGTTAGGTTAAACT 21504 GCAAGCTTGTCAGGTTAAACT bags7d17 ACTTGTACAAAGGCAAGCTTG 21505 ACTTGTACAAGGGCAAGCTTG bags7d17 CTTTAATATACGCTCGAAGCA 21506 CTTTAATATATGCTCGAAGCA bags7d17 CTATCATCTCGGAAATGTTTG 21507 CTATCATCTCAGAAATGTTTG bags7d17 TTGCCTGTGAAGTCTATCTTG 21508 TTGCCTGTGAAGTCTATCTTG bags7d17 CTAATCTGCAGCTCTGCTTTG 21509 CTAATCTGCAGCTCTGCTTTG bags7d17 GAAATGTTTGCTGGCAGCATG 21510 GAAATGTTTGTTGGCAGCATG baal9c20 CACACAAAATCTCACGATGTC 21511 CACACAAAATTTCACGATGTC baal9c20 GTATGGGGCAAGGAGGGAAGA 21512 GTATGGGGCATGGAGGGAAGA

TABLE 27-14 Haruna Niio SEQ Clones H602 ID NO: baal9c20 TGTTACCGTGATTTCTCATCT 21513 TGTTACCGTGGTTTCTCATCT bags20j08 TGTTTGTATCATTACTTGTAG 21514 TGTTTGTATCCTTACTTGTAG bags20l05 TAACGAGTCATCCACCGACGG 21515 TAACGAGTCAGCCACCGACGG bags20l05 TTGTAAGTTCGACAGGTTACT 21516 TTGTAAGTTCCACAGGTTACT bags20l05 CCAGTACAAACGGGAAGTTAG 21517 CCAGTACAAAAGGGAAGTTAG bags20l05 CACCTGATGCGACAGGATGCT 21518 CACCTGATGCAACAGGATGCT baal15j23 AAGTCATCAGTTGCTTACCAG 21520 AAGTCATCAGATGCTTACCAG baal15j23 CATAACATTTGATTTGAACAA 21521 CATAACATTTTATTTGAACAA baal15j23 TGTGTGTGTGTGCGCGCTTGG 21522 TGTGTGTGTGCGCGCGCTTGG baal15j23 CGTATTGGATTTGGTCAGAGT 21523 CGTATTGGATGTGGTCAGAGT baal15j23 TAACATGAGAAAGGATATATT 21524 TAACATGAGAGAGGATATATT BaGS37E11 GACGGTCACCAAGGGGAAACT 21528 GACGGTCACCGAGGGGAAACT bah50l14 GCTCCTGGCCCGTTCGCTGCA 21529 GCTCCTGGCCTGTTCGCTGCA BaSD27E20 GCTCCATGGCAGTGTTCACGT 21530 GCTCCATGGCCGTGTTCACGT BaSD27E20 CGGCGTTGTCGAGGGTATTGC 21531 CGGCGTTGTCAAGGGTATTGC basd17f18 TATATATACCGTAGTTTGTAA 21532 TATATATACCATAGTTTGTAA basd17f18 ATCCCTAAAAAGGGATAATTT 21533 ATCCCTAAAATGGGATAATTT basd17f18 CACCGCCGCTGTTCCCCGCAG 21534 CACCGCCGCTGTTCCCCGCAG basd17f18 GAAATACTCAGAAATAAACCA 21535 GAAATACTCAAAAATAAACCA basd17f18 GAGATTGTGTTGACAGAAATA 21536 GAGATTGTGTCGACAGAAATA basd17f18 AACAGGTGACTAATGAGATTG 21537 AACAGGTGACCAATGAGATTG BaSD12L12 CCGACCATGTTGATAATGANN 21538 CCGACCATGTGNATAATGANN baal32m04 TGTTACCGTAGATGTTACCTC 21539 TGTTACCGTAAATGTTACCTC baal32m04 TTCACTTGGGAAAGACATCTC 21540 TTCACTTGGGGAAGACATCTC baal32m04 AGGAGTTGATCATCTGTCAGG 21541 AGGAGTTGATGATCTGTCAGG

TABLE 27-15 Haruna Niio SEQ Clones H602 ID NO: baal32m04 CTGCGTGCCAGATCCTGCTCT 21542 CTGCGTGCCAGATCCTGCTCT baal32m04 CTGCTCTAGTAATAACAGGGT 21543 CTGCTCTAGTCATAACAGGGT BaAK24G10 ATGCTTAACACAAGTCATATC 21547 ATGCTTAACATAAGTCATATC bah50f16 ATCTCACTGTCATATATCTTT 21549 ATCTCACTGTTATATATCTTT bah50f16 CAACAAAGCAAATTGCCTGTG 21550 CAACAAAGCATATTGCCTGTG bah50f16 CCTTCGGCCTACTAGGTATAT 21551 CCTTCGGCCTGCTAGGTATAT bah50f16 AGCTTGGTGCGCCAAAATCCC 21552 AGCTTGGTGCACCAAAATCCC basd17d11 ACCACCTCAAGAGAACGCGCA 21553 ACCACCTCAATAGAACGCGCA basd17d11 ATGCTTGAGAATTGTAACCGT 21554 ATGCTTGAGACTTGTAACCGT bah37p24 GCATTGGTAGTCAAATGTCAT 21555 GCATTGGTAGCCAAATGTCAT bah37p24 AGCAGAGCAGCTTACGGAAGA 21556 AGCAGAGCAGATTACGGAAGA baet45C1105 CAGGATGGACGGTGAAACATA 21557 CAGGATGGACTGTGAAACATA baet45C1105 AGAAAAAGAAGAACTTTCAGA 21558 AGAAAAAGAAAAACTTTCAGA baet45C1105 ATTATCTGTACGTACATAACG 21559 ATTATCTGTATGTACATAACG baet45C1105 GAAACCGAAGGGCGGCTAGTC 21560 GAAACCGAAGTGCGGCTAGTC baet45C1105 ATTTGTCCTCAGGGTTCATCT 21561 ATTIGTCCTCGGGGTTCATCT bah47l21 TTGCCTTGAAGGCAAACCTTG 21562 TTGCCTTGAATGCAAACCTTG bah47l21 CATTCTCTTCGTGCGAAACTA 21563 CATTCTCTTCATGCGAAACTA bah47l21 TCCACCCGTCGGTCCTTTGCT 21564 TCCACCCGTCTGTCCTTTGCT bah47l21 ACACCTCGCCATCGTCGCCGC 21565 ACACCTCGCCGTCGTCGCCGC baak14b08 TGTGACATGATAGCTCACCTC 21569 TGTGACATGAGAGCTCACCTC baak14b08 CACAATCTGCGTCATACAGAA 21570 CACAATCTGCATCATACAGAA baakl4b08 AGCCTTTTGTATTTCATAGAA 21571 AGCCTTTTGTGTTTCATAGAA baakl4b08 GAGAGTAGAAGAGAGAGAGCA 21572 GAGAGTAGAAAAGAGAGAGCA bah54n10 CCGTGGCGTAACGGCACCTTT 21573 CCGTGGCGTAGCGGCACCTTT

TABLE 27-16 Haruna Niio SEQ Clones H602 ID NO: bah54n10 CAGTTTGGTTACGAAAGGTAA 21574 CAGTTTGGTTTCGAAAGGTAA bags34p08 CGCGGTTGCCATGCAACATGT 21576 CGCGGTTGCCGTGCAACATGT bags34p08 ACCGGCCAGAAAAAAAATCCC 21577 ACCGGCCAGGGAAAAAATCCC baal1e06 ATCATCACCTCGTAGCAGGAT 21578 ATCATCACCTTGTAGCAGGAT baal1e06 GAGCACAGGCCAGANAATTAC 21579 GAGCACAGGCNAGANAATTAC baak14k12 GCATCCGCTGAGGCTGGTGCA 21581 GCATCCGCTGGGGCTGGTGCA baak14k12 GCACCCTTGTTATCGTCCAAC 21582 GCACCCTTGTCATCGTCCAAC baal6a21 ACCCGATCGGAAGCGGCGCCA 21588 ACCCGATCGGCAGCGGCGCCA baal29l09 CCACTGCCAAATTTCAAGAAG 21593 CCACTGCCAAGTTTCAAGAAG baal29l09 TAAGAAGACTTCATCCACAGT 21591 TAAGAAGACTGCATCCACAGT bastl47C0606 ATTTCTGAATTCCTCTTCCTC 21592 ATTTCTGAATGCCTCTTCCTC kr66d1107 TAATTTCAATGTCACATCCAG 21593 TAATTTCAATTTCACATCCAG bah50m03 TGGGCGTGGTGGGCAACCCGT 21594 TGGGCGTGGTCGGCAACCCGT basd27k17 GTGAGCGATAGGCATGAGCAT 21595 GTGAGCGATATGCATGAGCAT basd27k17 AGGATCACCCGGGCTTTCCTC 21596 AGGATCACCCCGGCTTTCCTC basd27k17 ACCTCCATGTCTGACCTGACA 21597 ACCTCCATGTGTGACCTGACA

TABLE 28-1 Haruna Niio SEQ Clones H602 ID NO: basd2p19 ATAGCAGCAGGATCACTTCAC 21598 ATAGCAGCAG-ATCACTTCAC 21599 bags6f02 CTGAGACTGCGGGCGACACAC 21601 CTGAGACTGCAGGCGACACAC bags6f02 CGACACACGCGACAAGAACAG 21602 CGACACACGCAACAAGAACAG bags6f02 GGGTCCGCCGCCGCAGCCCCC 21603 GGGTCCGCCGTCGCAGCCCCC bags6f02 CACGCCCACCGGGCTCTGCAG 21604 CACGCCCACCAGGCTCTGCAG baak39j07 CGTCCATGGATACAGAACATG 21605 CGTCCATGGACACAGAACATG BaAK1L23 AANATGTGATTCCTATANNNN 21606 AANATGTGAT-CCTATANNNN 21607 BaAK1L23 CCACTCTTGANCACATCTTCA 21608 CCACTCTTGAGCACATCTTCA BaAK1L23 TCTTGATGACAATCATGTGTT 21609 TCTTGATGACGATCATGTGTT baak17e10 CCAGCATGATGTTGATGGAGG 21610 CCAGCATGATATTGATGGAGG baak17e10 TTTGGTGTAAGAAGGAAGAAA 21611 TTTGGTGTAACAAGGAAGAAA baak17e10 TGCAGGTGCCGCCCAGCATGA 21612 TGCAGGTGCCTCCCAGCATGA bah50h08 TGCTGGTTCCCGGGATCGAGC 21613 TGCTGGTTCCGGGGATCGAGC bah50h08 CTCCGTGCGGCGGCTGGTCAG 21614 CTCCGTGCGGTGGCTGGTCAG baall7e10 TCATGCTCGTTTACGAGTATC 21615 TCATGCTCGTCTACGAGTATC basd17c05 ATTTCATTTACAAAAACATAC 21616 ATTTCATTTATAAAAACATAC basd17c05 ACCTCTGTACCATAATATAAG 21617 ACCTCTGTACCATAATATAAG basd17c05 AAAGAACACAGACAAAAAACC 21618 AAAGAACACATACAAAAAACC basd17c05 GAAGCATTGATTCAAAGCCTG 21619 GAAGCATTGAATCAAAGCCTG basd17c05 AGGCGTGTCACAGAGAAGCAT 21620 AGGCGTGTCATAGAGAAGCAT bags14p02 CCCCCGCCGCGGCATAGATGG 21621 CCCCCGCCGCTGCATAGATGG bags14p02 TTGTTGAACAGGTAGACCCCC 21622 TTGTTGAACAAGTAGACCCCC bastl42H0115 TTGTTCATCCCATCACATCCC 21623 TTGTTCATCCAATCACATCCC bastl42H0115 CCGACATCATATTATTTATCT 21624 CCGACATCATGTTATTTATCT baak19c08 TTGTTGTAAGTGATCAGTCTA 21625 TTGTTGTAAGCGATCAGTCTA

TABLE 28-2 Haruna Niio SEQ Clones H602 ID NO: baak19c08 CGCCCATCACGAAGCTCTGCA 21626 CGCCCATCACAAAGCTCTGCA bastl40h1115 GCTCACCGCCGTGACGAAAAC 21627 GCTCACCGCCATGACGAAAAC bastl40h1115 CTTGAAATGCGTAGATTTGTG 21628 CTTGAAATGCATAGATTTGTG BaAK21P12 GGATCGCCCCGCCCTCTGGAA 21632 GGATCGCCCCACCCTCTGGAA bast79C0406 AATCAAACTCCAGAGAAGGAA 21635 AATCAAACTCAAGAGAAGGAA bah45m08 TAGGCGGCGATGCTGCAACAT 21636 TAGGCGGCGACGCTGCAACAT kr28a0301 CTATTCAGTCCCCTCCTACAA 21637 CTATTCAGTCACCTCCTACAA kr28a0301 TTTGGTTCTCGCCTTTGTTTA 21638 TTTGGTTCTCACCTTTGTTTA kr28a0301 CCAGTTTTAGCTTCTCATGTG 21639 CCACTTTTAGGTTCTCATGTG kr28a0301 CTCCCCTGTCGGTGGATTATA 21640 CTCCCCTGTCAGTGGATTATA kr28a0301 TTGTGTTGTTATGTTAGGATT 21641 TTGTGTTGTTGTGTTAGGATT kr28a0301 GGCATGTTTTGAATGTGAAAA 21642 GGCATGTTTTCAATGTGAAAA baak21l22 AACAGCTAAGCGTATTTGGGC 21646 AACAGCTAAGGGTATTTGGGC baak21l22 TTTCAGAAGCGGATTGAAACT 21647 TTTCAGAAGCAGATTGAAACT baak21l22 CTTTAGAAGAGCTACCTCACC 21648 CTTTAGAAGAACTACCTCACC baak21l22 TTCAGGCACAGACATCAGGTA 21649 TTCAGGCACAAACATCAGGTA basd15h01 GGTTGATGGCGACCCACAGCA 21650 GGTTGATGGCAACCCACAGCA basd15h01 GGCCCTTGGCGAACGGGTACA 21651 GGCCCTTGGCCAACGGGTACA basd15h01 AGTTCTGGGATGGCGGGTTGA 21652 AGTTCTGGGACGGCGGGTTGA basd15h01 CGGCGAACTCATCGTTTTCGT 21653 CGGCGAACTCGTCGTTTTCGT bah19o11 CAGCAGACAAGATGGGGGAAG 21654 CAGCAGACAATATGGGGGAAG bah19o11 TGACGAGGGGAGCGGGCTCAG 21655 TGACGAGGGGCGCGGGCTCAG bah19o11 ATCATCATCACCATCAATCTC 21656 ATCATCATCATCATCAATCTC bags39l05 ACTGCGAGGATCTTAGCAGTA 21658 ACTGCGAGGAGCTTAGCAGTA bags39l05 CTGATAGATAGGGAGTTTACC 21659 CTGATAGATATGGAGTTTACC

TABLE 28-3 Haruna Niio SEQ Clones H602 ID NO: bags39l05 CAAGCAAACAGCCAATGGTCA 21660 CAAGCAAACAACCAATGGTCA bags39l05 AGCAGAAAAACAATGTTTAAT 21661 AGCAGAAAAATAATGTTTAAT BaH58P03 TTGTTGTTGTCCACGGAAGAA 21663 TTGTTGTTGTACACGGAAGAA BaH58P03 ATCAAGATTGTTAGCAGGACG 21664 ATCAAGATTGGTAGCAGGACG BaH58P03 ACCACTTGAAATATCTTACTT 21665 ACCACTTGAAGTATCTTACTT BaAL4O04 TTATTGTGGTTGGGGTGGTGG 21667 TTATTGTGGTCGGGGTGGTGG BaAL4O04 GATGGATGGCGGAAGGGCGGG 21668 GATGGATGGCAGAAGGGCGGG BaAL4O04 CACAACACAAACGATGAAAGA 21669 CACAACACAAGCGATGAAAGA baak14c23 AGTAGGCCATACTGGCTGGCG 21671 AGTAGGCCATGCTGGCTGGCG baak14c23 TCCAGTTTGCGAAGGACGGCT 21672 TCCAGTTTGCCAAGGACGGCT kr61A1101 CTATAAACACAAACAAGACTC 21673 CTATAAACACGAACAAGACTC kr61A1101 TCGCAAATATAAGAAAGAAGC 21674 TCGCAAATATTAGAAAGAAGC BaH42J17 GATGGATGGCGGAAGGGCGGG 21675 GATGGATGGCAGAAGGGCGGG BaH42J17 TTATTGTGGTTGGGGTGGTGG 21676 TTATTGTGGTGGGGGTGGTGG BaH42J17 CGAGCTTCCTGTGGAGGAGAG 21677 CGAGCTTCCTTTGGAGGAGAG bah58h08 TGTTGGACAGGAAATCGTACG 21678 TGTTGGACAGCAAATCGTACG bah58h08 GTACGTGACAGCTGACAAGAC 21679 GTACGTGACAACTGACAAGAC bast78a0202 GCGCTCTCCCGGAGCTCCAGC 21680 GCGCTCTCCCAGAGCTCCAGC BaSD18O16 TGCTTCTGCATATATGTCTTA 21682 TGCTTCTGCACATATGTCTTA baak22j11 GCTCACATCAGTGACCGCCGC 21683 GCTCACATCAATGACCGCCGC baak22j11 GTGGAGGCGGTGCTCCATGGC 21684 GTGGAGGCGGGGCTCCATGGC baak22j11 CCGCCTCCGTCTTCTCGGCCA 21685 CCGCCTCCGTTTTCTCGGCCA baak22j11 CCCGCTCGTGCGCCAGCTCAC 21686 CCCGCTCGTGAGCCAGCTCAC baak20k04 GGCTCGCAATGCCCAGGTACC 21687 GGCTCCCAATTCCCAGGTACC baak20k04 ATCAAGCCGGACAGCAGCCAG 21688 ATCAAGCCGGCCAGCAGCCAG

TABLE 28-4 Haruna Niio SEQ Clones H602 ID NO: bags29l11 CCTCCTCTTCATCATCATCAA 21689 CCTCCTCTTCATCATCATCAA bags29l11 TAAACAAAAATCAAAAGGGTA 21690 TAAACAAAAAACAAAAGGGTA baak14o13 ACAGATCAGGGTAAGGCATTT 21691 ACAGATCAGGTTAAGGCATTT baak14o13 CAATTTACAACCTTCCAAAGA 21692 CAATTTACAATCTTCCAAAGA baak14o13 GGGGCATGACCAATATGAACA 21693 GGGGCATGACTAATATGAACA bags28k04 CAGCGTGCGGTATGCCACATC 21696 CAGCGTGCGGGATGCCACATC bags28k04 CATCCTTTTATGTTGATTCAT 21697 CATCCTTTTACGTTGATTCAT bags28k04 TGCTTGCCCAGCAGCAGCGCC 21698 TGCTTGCCCAACAGCAGCGCC BaGS37M03 AAATAGCTCACACCAGTGATT 21701 AAATAGCTCATACCAGTGATT bags18c14 CATGGTTTGCCATGTTAGATA 21702 CATGGTTTGCTATGTTAGATA bast23G0214 TAATTTGCAAATCCCAAGTGA 21703 TAATTTGCAAGTCCCAAGTGA bast23G0214 GGGTGACGACGCGTGAAAAAC 21704 GGGTGACGACTCGTGAAAAAC bast23G0214 ACACATGCATGCGCATCCTGG 21705 ACACATGCATACGCATCCTGG bah47p02 TCATCTGGGCGCCTCTATGCC 21706 TCATCTGGGCACCTCTATGCC bah47p02 CAACTTCTACTTACATACTTG 21707 CAACTTCTACGTACATACTTG kr66h0216 AAAAAAAAAAAAAAAAAAAAA 21712 AAAAAAAAAACAAAAAAAAAA kr66h0216 TTTTACAACTCGCAACACAAT 21713 TTTTACAACTGGCAACACAAT BaG323D15 ACCCACAAAATCCTCCAATTA 21714 ACCCACAAAAGCCTCCAATTA BaGS23D15 GCGTTTAGAGTATTTACACCA 21715 GCGTTTAGAGCATTTACACCA BaG323D15 ACAAATGTAAGCAAAAACCAG 21716 ACAAATGTAATCAAAAACCAG baal22c17 ACATTGTTTAITGTGACAAAT 21717 ACATTGTTTAATGTGACAAAT baal22c17 ACCCACAAAAGCCTCCAATTA 21718 ACCCACAAAATCCTCCAATTA bags34l17 ATGTAACTCTACATGGCAAAG 21720 ATGTAACTCTGCATGGCAAAG bags34l17 GGTTGACTAATAGAACTCCAG 21721 GGTTGACTAACAGAACTCCAG bags34l17 CTAATGCTACCTTAAGTCCCA 21722 CTAATGCTACATTAAGTCCCA

TABLE 28-5 Haruna Niio SEQ Clones H602 ID NO: bags34l17 CCAGAGATTACCAGCCAAATT 21723 CCAGAGATTAGCAGCCAAATT bags34l17 TTAAGTCCCATGTAAGCCCTT 21724 TTAAGTCCCAGGTAAGCCCTT bah14j16 GCAGAGAGCAGAAATAGGTGC 21726 GCAGAGAGCAAAAATAGGTGC BaAK21B17 TCCTTTTGATACCGAGGTCGG 21727 TCCTTTTGATGCCGAGGTCGG baak21m13 TGGCTCTATGAAGGAATCTTC 21729 TGGCTCTATGTAGGAATCTTC baak21m13 ATTTAGATGAGTCGACTATAC 21730 ATTTAGATGAATCGACTATAC baak21m13 CATTCCTCATGTACCATTTAG 21731 CATTCCTCATATACCATTTAG baak21m13 AAAATAATTCTGTACTTCTGG 21732 AAAATAATTCAGTACTTCTGG baak21m13 GTTTTACCTGGTGCGGCCCTT 21733 GTTTTACCTGTTGCGGCCCTT baak21m13 ATGAGTTTAGGGCGTCGAGCT 21734 ATGAGTTTAGTGCGTCGAGCT baak21m13 TAAGGAAAAAATTCGCACTTG 21735 TAAGGAAAAATTTCGCACTTG BaH38E01 TGGTCACCTTGCACATGTCTG 21736 TGGTCACCTTACACATGTCTG bast03G0313 GATCGCAAGACGCGAACTGCT 21737 GATCGCAAGATGCGAACTGCT bast03G0313 GCGCTACAGAGACAGCCGGCG 21738 GCGCTACAGATACAGCCGGCG basd18g06 TCGCTCTAGGACTAGGACTAG 21739 TCGCTCTAGGTCTAGGACTAG basd18g06 ACTAGGACAGGATGGCCGGGT 21740 ACTAGGACAGAATGGCCGGGT baak40i22 AGGTTGGTACAAAATTTTGAA 21741 AGGTTGGTACGAAATTTTGAA baak40i22 CTGTGTTGGGGGATATACATG 21742 CTGTGTTGGGTGATATACATG baal2n12 CGACGACGACGATGAGGTTTT 21743 CGACGACGACAATGAGGTTTT baal2n12 ATGCATGCACGGACTGAGGTG 21744 ATGCATGCACAGACTGAGGTG baal2n12 ATAATAATAACATAATGATAA 21745 ATAATAATAATATAATGATAA BaSD3C24 CGCGATGTCACAATCTCGTCA 21746 CGCGATGTCATAATCTCGTCA baak46m07 ACAAAACTGGAATTAGAATGT 21747 ACAAAACTGGGATTAGAATGT baak46m07 CTAGACCGCTGATCTCAGTTT 21748 CTAGACCGCTCATCTCAGTTT baak46m07 TCTTCTGTTCCATGACCGCCT 21749 TCTTCTGTTCAATGACCGCCT

TABLE 28-6 Haruna Niio SEQ Clones H602 ID NO: baak46m07 TGGAGAAGCCTGTTCACTATG 21750 TGGAGAAGCCAGTTCACTATG BaGS17N04 TTCTTAAGAAGGTAGTCGGTG 21751 TTCTTAAGAATGTAGTCGGTG BaGS17N04 CTCTACCAATGCCTAGGGGCC 21752 CTCTACCAATACCTAGGGGCC BaGS17N04 GACATTCCGATGGAACTAGCC 21753 GACATTCCGAGGGAACTAGCC bags12d09 TAGGTACATCTATACGCAACT 21754 TAGGTACATCGATACGCAACT basd20c08 GGAACCGCCCGTCCGAGGAGT 21755 GGAACCGCCCATCCGAGGAGT basd20c08 GACACCGTCCAGGTGCCACGC 21756 GACACCGTCCGGGTGCCACGC basd20c08 GTCCCACACCTGGCAGGTCTT 21757 GTCCCACACCGGGCAGGTCTT baak15p03 GACGAGGTGAGGGCTACTGGT 21759 GACGAGGTGATGGCTACTGGT baak15p03 TACTGGTCATGGTCTGAACTC 21760 TACTGGTCATCGTCTGAACTC baal18g11 GTCCATTGGACTTCGATTGAA 21761 GTCCATTGGATTTCGATTGAA baak40g02 GTTTCAGGAAATTCTACAGTT 21764 GTTTCAGGAAGTTCTACAGTT BaH26B16 CGAAGATGATTGCCGCACATC 21765 CGAAGATGATCGCCGCACATC baal5n08 TCGGGACATAGCTGGTGGCGG 21766 TCGGGACATATCTGGTGGCGG baal5n08 TTTTTACAGGGGACTTGCTTC 21767 TTTTTACAGGTGACTTGCTTC bags9c08 CATCTGCAAGTATAAAGACAA 21768 CATCTGCAAGCATAAAGACAA bags9c08 ATAAAGACAACGGTCAGTCAA 21769 ATAAAGACAATGGTCAGTCAA basd1j22 ATGCACACTGGATGAAGGATG 21772 ATGCACACTGCATGAAGGATG basd1j22 AGGTAAATAGGGTGTGCGAGT 21773 AGGTAAATAGAGTGTGCGAGT basd1j22 TTGAAACCAGTGAGTGTGTTT 21774 TTGAAACCAGGGAGTGTGTTT basd1j22 AGCTAAGGGCAGTCTACTTCT 21775 AGCTAAGGGCGGTCTACTTCT BaH23N03 ACTTTTATTACGGAATTGAGA 21776 ACTTTTATTATGGAATTGAGA bast46d0808 ATGCTCAGATGATGAGCATTT 21777 ATGCTCAGATCATGAGCATTT bast22a0802 TCTTGTGCCTTCCACCTCGCC 21778 TCTTGTGCCTGCCACCTCGCC bast34d0808 TCTACACACACAAAAAATACT 21779 TCTACACACAAAAAAAATACT

TABLE 28-7 Haruna Niio SEQ Clones H602 ID NO: bast48e0509 ATGACACAGACGGATTCAACA 21780 ATGACACAGATGGATTCAACA bast48e0509 AGTTGGATAATGTGTTCATGA 21781 AGTTGGATAACGTGTTCATGA bast26f1012 AGAAAGAATGGACACATGTAT 21782 AGAAAGAATGGACACATGTAT baal30e07 TCTCCATTACGAGCTTCATGT 21783 TCTCCATTACCAGCTTCATGT baal30e07 CCTCTTCACTAGGATCTCCAC 21784 CCTCTTCACTGGGATCTCCAC baal30e07 TAGTCTTCATGGCCTCTGACT 21785 TAGTCTTCATAGCCTCTGACT baal30e07 TATTTAAGCACTCATGTGATC 21786 TATTTAAGCAGTCATGTGATC bags18i05 TGGTTTGCTCATGCAATTCTA 21787 TGGTTTGCTCGTGCAATTCTA bags8o06 CGATCACTCGTCCTTTGGTTA 21793 CGATCACTCGCCCTTTGGTTA baak46e14 ACCTGCACGGAAGATGCGCCG 21794 ACCTGCACGGCAGATGCGCCG baak46e14 TAGCGGCGTCGTCACGTGATC 21795 TAGCGGCGTCATCACGTGATC baak46e14 GTGTAAGCAACTTACTGTTAT 21796 GTGTAAGCAAGTTACTGTTAT baak46e14 CAGGACATAGTCCCTCATGAG 21797 CAGGACATAGGCCCTCATGAG BaGS38N08 TCTGTAAAACCAACATCAGGT 21798 TCTGTAAAACTAACATCAGGT BaH49P17 TAGGATCCTGCGTGTAGAAGG 21799 TAGGATCCTGTGTGTAGAAGG BaH49P17 GGGGTATCTGCCTGCGGCCAT 21800 GGGGTATCTGTCTGCGGCCAT bags21d11 GTAAGAAAGAAGATTGTCACT 21805 GTAAGAAAGAGGATTGTCACT bags21d11 TTTTATTTCCTGCTTTGTAAG 21806 TTTTATTTCCCGCTTTGTAAG kr39H0816 TCGTGCAGGCCTGAACAGGTG 21807 TCGTGCAGGCTTGAACAGGTG kr39H0816 GTCTCGTCTCCCCCATCCTGC 21808 GTCTCGTCTCCCCCATCCTGC kr39H0816 CCCTGGACTAGTGAGGAAGAC 21809 CCCTGGACTACTGAGGAAGAC kr67d0208 CAAATTCAGCATAAGGCGGTA 21814 CAAATTCAGCGTAAGGCGGTA baak1h16 TTCGTGTAGTAGCGAGATGTG 21815 TTCGTGTAGTGGCGAGATGTG BaH26F10 GACGCTTAGCCTCTCTCTCCG 21816 GACGCTTAGCTTCTCTCTCCG bags14n02 AGTCAACCGAGACGCACACAA 21817 AGTCAACCGAAACGCACACAA

TABLE 28-8 Haruna Niio SEQ Clones H602 ID NO: bah36f01 CAGTTCCTCTAGAACAGAGCT 21818 CAGTTCCTCTGGAACAGAGCT bah36f01 CGGGTGTAGTTGCACCAGCCG 21819 CGGGTGTAGTGGCACCAGCCG bast79d1107 ATGTTCATGCGTCTCTCTATA 21820 ATGTTCATGCATCTCTCTATA bast79d1107 TCTATATACAGACTAAATGTC 21821 TCTATATACAAACTAAATGTC bast79d1107 ACTTGAAAAAGAAAATGTGAT 21822 ACTTGAAAAAAAAAATGTGAT bast79d1107 CTTACCTCCAGACCGCAGTCA 21823 CTTACCTCCATACCGCAGTCA bast79d1107 GTACAAAGTCTGAATGTTCAT 21824 GTACAAAGTCCGAATGTTCAT BaGS26O20 CAACAGATCAAGCTTTCGTAG 21825 CAACAGATCATGCTTTCGTAG BaGS26O20 GCGCAGCTGAGAAATGACATT 21826 GCGCAGCTGACAAATGACATT BaGS26O20 AAGCTCAACCATAGCCTTCTT 21827 AAGCTCAACCCTAGCCTTCTT BaGS26O20 GGGCTTCATACCATCAAGCTC 21828 GGGCTTCATAACATCAAGCTC BaGS26O20 CCAACATCAAAGTGTGGTTCG 21829 CCAACATCAAGGTGTGGTTCG BaGS26O20 CATCGAGGCAAACTTCTTAAA 21830 CATCGAGGCAGACTTCTTAAA bags22h11 TATTTCAACATGCAGTGCAAC 21832 TATTTCAACACGCAGTGCAAC bags22h11 GAAATATTACCAATAGCACAC 21833 GAAATATTACGAATAGCACAC baet19D0608 CGGCTAGGCCGAGTGGGTCAA 21834 CGGCTAGGCCAAGTGGGTCAA bags23a14 TCGCTCCCACATCTGAAGTTC 21835 TCGCTCCCACGTCTGAAGTTC bags23a14 CAGGTGCTGGTTCAGTGCTCA 21836 CAGGTGCTGGGTCAGTGCTCA bags37c09 CATCGTCAAAGTTATTAAACA 21837 CATCGTCAAAATTATTAAACA bags37c09 AAAAGTGAGTGACAAGATTTC 21838 AAAAGTGAGTTACAAGATTTC bags20a01 TGATTGGGCGTTTCAAGTGGG 21839 TGATTGGGCGCTTCAAGTGGG bags20a01 CATCAAATATGGTCTGATCAG 21840 CATCAAATATTGTCTGATCAG bags20a01 GCCGGTAGGCAATGTGCTGGA 21841 GCCGGTAGGCGATGTGCTGGA bags20a01 GGCATACCGCGGACGACCGGC 21842 GGCATACCGCTGACGACCGGC bags20a01 AGAAAAGGACGCGGCATACCG 21843 AGAAAAGGACGCGGCATACCG

TABLE 28-9 Haruna Niio SEQ Clones H602 ID NO: bah53m11 GCGCGTCGCAGACGAACTCCC 21844 GCGCGTCGCATACGAACTCCC bah53m11 CGAAGAAGTTGGTCTTGCCCT 21845 CGAAGAAGTTTGTCTTGCCCT bah53m11 ATCACTCCGTCTAACTGTCAC 21846 ATCACTCCGTGTAACTGTCAC bags26l01 GACTCAAGACGCAGATAAATT 21847 GACTCAAGACACAGATAAATT bags26l01 TTATTGACGGGCAAGCGAAGC 21848 TTATTGACGGCCAAGCGAAGC bags21p23 AACACACCTTGCACATTCATT 21849 AACACACCTTCCACATTCATT baak24b01 ACTTACAGCGCCTACAGAATG 21850 ACTTACAGCGGCTACAGAATG bastl56C0105 TTAATCTGGAGTTTTTGAAGG 21852 TTAATCTGGATTTTTTGAAGG bastl56C0105 CTGATTACAACGCACCAATTA 21853 CTGATTACAAGGCACCAATTA bags28d11 TCGGGCATCACCAGTCACAGC 21855 TCGGGCATCAACAGTCACAGC bags28d11 AAACCTGATATCCAAATGAGG 21856 AAACCTGATAGCCAAATGAGG bags28d11 AGAAGAAATCGAGTCGTCTTG 21857 AGAAGAAATCAAGTCGTCTTG baak11d12 AGAGTTTTACGAGATACCCAT 21858 AGAGTTTTACAAGATACCCAT BaH42A07 CAACTCTACAGTTTTTTTGTA 21859 CAACTCTACATTTTTTTTGTA BaH42A07 ACCTAACAGTTGGCGCCAACG 21860 ACCTAACAGTGGGCGCCAACG bah60f18 CTACCATCCATCCAACAATAT 21863 CTACCATCCATCCAACAATAT bah60f18 TCATTGATCAACTCCTTCACC 21864 TCATTGATCAGCTCCTTCACC bah60f18 TAGCACAATCAATTACCACAT 21865 TAGCACAATCTATTACCACAT bah60f18 AATATTTTCCGCACTTTTAAG 21866 AATATTTTCCACACTTTTAAG baal2n10 GTCAATAGAT-CACGGCGTGG 21867 GTCAATAGATACACGGCGTGG 21868 baal2n10 TGTGGGAGGATGCGTCTTCCT 21869 TGTGGGAGGACGCGTCTTCCT baak32c23 CTGGACCATCGATAGACCATA 21876 CTGGACCATCAATAGACCATA baak32c23 GAATTTCTCACAGCATACGGT 21877 GAATTTCTCAAAGCATACGGT baak32c23 ATGCAGCGTCCGAGGGGTTGG 21878 ATGCAGCGTCGGAGGGGTTGG baak32c23 AATTGTAGTCTCAGAGATGTG 21879 AATTGTAGTCCCAGAGATGTG

TABLE 28-10 Haruna Niio SEQ Clones H602 ID NO: baal9e06 ACCATCTATAGAGTTTCCTTA 21880 ACCATCTATATAGTTTCCTTA baal9e06 TACTTATGCGCGCGTAGATTA 21881 TACTTATGCGAGCGTAGATTA bastl47E0309 CCCAAAGCCGTCGCTCTGGCA 21883 CCCAAAGCCGCCGCTCTGGCA bastl47E0309 CAACCGAAGCAGACACCCTAC 21884 CAACCGAAGCTGACACCCTAC bastl47E0309 ATCTCCGAAGCCGTCACTTCG 21885 ATCTCCGAAGTCGTCACTTCG bastl47E0309 CCCTGCTGTCTAAGCAACCGA 21886 CCCTGCTGTCCAAGCAACCGA baak30o08 GGGTCAGAAGAAGCATTCATA 21887 GGGTCAGAAGGAGCATTCATA baet44C0606 TGACCATGACGTCCTGCTTCA 21888 TGACCATGACATCCTGCTTCA baet44C0606 CCTTGAGGAGGAACGGCTTGA 21889 CCTTGAGGAGAAACGGCTTGA baet44C0606 TGTCCTTGAGCGTCGTCTCCT 21890 TGTCCTTGAGAGTCGTCTCCT bags39g18 GCTGATCGCATGTTTTGCGGT 21891 GCTGATCGCACGTTTTGCGGT bags38m06 AGCATGTATCGATGCAGCACA 21893 AGCATGTATCAATGCAGCACA bags38m06 CATGGTGGTTTACACTTAGAT 21894 CATGGTGGTTCACACTTAGAT bags38m06 TATGAAACATAGGATATACAT 21895 TATGAAACATGGGATATACAT bags38m06 AGAGAATGTCTTAGTGACCAG 21896 AGAGAATGTCATAGTGACCAG bags3i04 TCGAATCTGCCATCCGTAATC 21897 TCGAATCTGCAATCCGTAATC bags3i04 TTCAAAAAATTATGTCCACGT 21898 TTCAAAAAATCATGTCCACGT bags3i04 ACATGGAATGATACCCTCCAT 21899 ACATGGAATGTTACCCTCCAT baal1a11 TTTCAACATCGCTTACCTCCC 21900 TTTCAACATCACTTACCTCCC basd2m23 CTCACAAACAGTTTGCACAAA 21901 CTCACAAACAATTTGCACAAA basd12k23 ACATGAACCTTGCATTGCACA 21905 ACATGAACCTCGCATTGCACA basd12k23 GTCTGGAGCTACCGGTTGCTG 21906 GTCTGGAGCTCCCGGTTGCTG basd12k23 ATTCATAGCAGCTTGAAGATT 21907 ATTCATAGCAACTTGAAGATT bast22c0105 AAAAAACTTCGCTTCCAAAAG 21908 AAAAAACTTCCCTTCCAAAAG bast22c0105 ACCTCCTTAGTGCCACCACGA 21909 ACCTCCTTAGGGCCACCACGA

TABLE 28-11 Haruna Niio SEQ Clones H602 ID NO: BaGS38H20 AGTGCAACTGCACATAAAAAT 21910 AGTGCAACTGTACATAAAAAT BaGS38H20 TATACATTCCTTACAATGTGT 21911 TATACATTCCATACAATGTGT bah61p21 GCTATGCTTGCAACACAACGC 21912 GCTATGCTTGTAACACAACGC basd11h13 AGGGTGAAAATAAAGGTCAAG 21913 AGGGTGAAAACAAAGGTCAAG basd11h13 ACTGAAAATGCTGCAGGGTGA 21914 ACTGAAAATGTTGCAGGGTGA basd11h13 ATATTTGCCGGTCCTTCGCTC 21915 ATATTTGCCGCTCCTTCGCTC basd11h13 GTTGCCATCACCCTGACTGTG 21916 GTTGCCATCAGCCTGACTGTG basd11h13 GTCATAGATGCTGAATGTCAA 21917 GTCATAGATGTTGAATGTCAA baal0f04 GGGCAGCAGCAATGCCACCTT 21918 GGGCAGCAGCGATGCCACCTT baal0f04 ATGTACAGCACAAACGTGAAT 21919 ATGTACAGCAAAAACGTGAAT baal0f04 TGGAAAAATCACAACATCGCA 21920 TGGAAAAATCGCAACATCGCA baal0f04 CACCTTCTTTCGTAGCAACTG 21921 CACCTTCTTTTGTAGCAACTG kr18e0810 CATTTTTTGCGATAAATTGCA 21922 CATTTTTTGCAATAAATTGCA bags24f02 TTATGAATTAATAATGAATAG 21924 TTATGAATTAGTAATGAATAG bags24f02 CCTAGAAATCTTCATGTTTTC 21925 CCTAGAAATCGTCATGTTTTC bags24f02 GTGTGGTCTACAATAGAAAAT 21926 GTGTGGTCTAGAATAGAAAAT baal19m12 CAAGGTACGTNACATGAGAAT 21927 CAAGGTACGTGACATGAGAAT baal19m12 GAATGATGGCNATGAATGACG 21928 GAATGATGGCAATGAATGACG baak2e06 AACTTTTTTCTTAATCAATTA 21929 AACTTTTTTCGTAATCAATTA baak2e06 ATTTCTGTAAGTAACTAATCC 21930 ATTTCTGTAAATAACTAATCC baak12l16 AATCATAATCATTGCTATAAC 21932 AATCATAATCGTTGCTATAAC baak29d01 TGTCACAGACATGCGGATCTT 21933 TGTCACAGACGTGCGGATCTT baak19n06 TAGTGATATCAGTGTGCATAT 21934 TAGTGATATCTGTGTGCATAT baak19n06 CATCCCTCCTCCCAATCATAT 21935 CATCCCTCCTCCCAATCATAT baak4i02 CCACCACCTCCATCAGCTTAC 21936 CCACCACCTCAATCAGCTTAC

TABLE 28-12 Huruna Niio SEQ Clones H602 ID NO: bah49k10 CACCAAATCAGTATCATTCAC 21937 CACCAAATCACTATCATTCAC bah49k10 AAAGACCCAAAGGGCAGATCA 21938 AAAGACCCAAGGGGCAGATCA baal2o03 TCCGCGTCCAATGGCTCAACC 21939 TCCGCGTCCAGTGGCTCAACC baak28g14 CATTCTCAGTGAATGCCCAGC 21940 CATTCTCAGTAAATGCCCAGC baak28g14 ACTTGACGCATAGATAAAACA 21941 ACTTGACGCAAAGATAAAACA baak28g14 AGAAAGATGAGCAGCCACTGG 21942 AGAAAGATGAACAGCCACTGG baak28g14 ACTGGTTCATAAAAAGGCTAC 21943 ACTGGTTCATTAAAAGGCTAC baak28g14 AATCGAGGATGTGAATCACAA 21944 AATCGAGGATGTGAATCACAA bags16e11 TCTGGAGAGAAGAAAGCGGGG 21946 TTGGAGAGATGAAAGCGGGG bagsl6e11 ACTGCGCACTAAATCGAAATA 21947 ACTGCGCACTTAATCGAAATA bags16e11 CCGTAGGGCTAAAATAAACCT 21948 CCGTAGGGCTGAAATAAACCT bags23g13 CCGCCGTGAGGGACAGCTTCT 21949 CCGCCGTGAGAGACAGCTTCT bags23g13 TATCCTGCAATGTCGCGTGCA 21950 TATCCTGCAACGTCGCGTGCA bags23g13 GTATGTGACATTAGTTCTCGA 21951 GTATGTGACAGTAGTTCTCGA bags23g13 TTTCGGGCGGACACGATGACT 21952 TTTCGGGCGGTCACGATGACT basd23c05 GCACAGACTCAAGACCTCTGA 21953 GCACAGACTCCAGACCTCTGA basd23c05 TGACCCCATCCAGAAAATGGG 21954 TGACCCCATCTAGAAAATGGG basd23c05 GCAGAATTTGTTGAATATGTT 21955 GCAGAATTTGCTGAATATGTT bah19f17 GAGGAGAATTGAACAGAGAAT 21956 GAGGAGAATTAAACAGAGAAT bah19f17 TGCTGTAAATTATCTACTCCA 21957 TGCTGTAAATCATCTACTCCA bags26g17 GGTCAAATTTTCCCACCTACG 21963 GGTCAAATTTGCCCACCTACG bags26g17 TGTATTTTGTGAGGGGGTTTC 21964 TGTATTTTGTAAGGGGGTTTC baal6j16 GCTGAGAAAGGAAACAGTTTC 21965 GCTGAGAAAGAAAACAGTTTC bags29b09 TATCATAATTGGGGCTTGCAT 21966 TATCATAATTTGGGCTTGCAT bags29b09 TAGTGTCGCAGTAGATGCTGC 21967 TAGTGTCGCACTAGATGCTGC

TABLE 28-13 Haruna Niio SEQ Clones H602 ID NO: bags29b09 ATCCAAGTATAAGAGCCACCA 21968 ATCCAAGTATGAGAGCCACCA baal20e03 GGGATATCAGACTCTGAATCA 21969 GGGATATCAGTCTCTGAATCA bags9l23 CAAATATACTGATACACATAG 21970 CAAATATACTTATACACATAG bags9l23 ATAGTCAAGAAAAACTTTCAT 21971 ATAGTCAAGAGAAACTTTCAT bags9l23 CAGAATAACACATTAGTGAAA 21972 CAGAATAACAAATTAGTGAAA bah32e22 GGATGGTTACCTCTCATCACT 21973 GGATGGTTACGTCTCATCGCT bah32e22 ACCTCTCATCACTCCCAATTT 21974 ACGTCTCATCGCTCCCAATTT baak17o14 CGACTGTTCAATGTTACTAGT 21975 CGACTGTTCAGTGTTACTAGT baak17o14 GATACCTGACGTCAGCCTTGG 21976 GATACCTGACATCAGCCTTGG BaH42O04 GAACAGGCTCGCCTCCTTTCC 21981 GAACAGGCTCACCTCCTTTCC bast74F0111 CAAGGAAGGAAACCCTAGATA 21982 CAAGGAAGGAGACCCTAGATA BaSD21F13 GTTCAGGGCCGGCCCAGCAGA 21983 GTTCAGGGCCAGCCCAGCAGA baal29l08 CTTTGGTCCGTGAGTTTGCTC 21986 CTTTGGTCCGCGAGTTTGCTC baak21a04 TTTGAGCATAGCTAACAGTGA 21988 TTTGAGCATATCTAACAGTGA baak21a04 GGGGAGGTCCGGCGCACCCTC 21989 GGGGAGGTCCAGCGCACCCTC bags37f18 TATACTGTTACTGACACTGAG 21990 TATACTGTTAATGACACTGAG baal12h12 ATCATGGAGATACTGCAAGGT 21991 ATCATGGAGAAACTGCAAGGT BaAK26F12 TGAGACCTCGTGCAGCGCCCT 21994 TGAGACCTCGGGCAGCGCCCT bags12b05 CGGAGAGGTCATGGTCAGGCT 21995 CGGAGAGGTCGTGGTCAGGCT bags12b05 TCAGCTGAGAAAAAACCCTGC 21996 TCAGCTGAGAGAAAACCCTGC baal12h24 TGATTGACACACATCTGATCA 21997 TGATTGACACGCATCTGATCA baal12h24 TAGGTGATATATTCCTTTTGG 21998 TAGGTGATATCTTCCTTTTGG baal35j16 TGTCGGCTGCGCGGCAGCCCG 21999 TGTCGGCTGCACGGCAGCCCG bags37c07 CGAAGTTGTAATTGCTCTTGG 22000 CGAAGTTGTAGTTGCTCTTGG bags37c07 AGACTGAGATTTCTGGAGAGC 22001 AGACTGAGATATCTGGAGAGC

TABLE 28-14 Haruna Nilo SEQ Clones H602 ID NO: baak45d23 GTGATGGACCCGAGGGCGACA 22002 GTGATGGACCAGAGGGCGACA bah42g09 GGGGGGGGGGGGGGGGGGGGG 22003 GGGGGGGGGGAGGGGGGGGGG bah42g09 GGCAAGTTCTTAGGAGCAAAG 22004 GGCAAGTTCTCAGGAGCAAAG bah42g09 ACATCGAGTCGTCAGGAATCC 22005 ACATCGAGTCATCAGGAATCC baal40p01 TTCCGGGCTGACGTCTGGACG 22006 TTCCGGGCTGTCGTCTGGACG baal40p01 ATGCCTTGAGAGATATATATA 22007 ATGCCTTGAGGGATATATATA baal40p01 ACAGTGAATAGCCCGATCGTG 22008 ACAGTGAATATCCCGATCGTG baal40p01 ATTAATCAGAACATCGAGTGG 22009 ATTAATCAGACCATCGAGTGG baal40p01 TAAGCAATCAGTGAAATAAAG 22010 TAAGCAATCAATGAAATAAAG baal40p01 TTTAACCATACTGATAATTTA 22011 TTTAACCATATTGATAATTTA baal40p01 CACTTGAAATGATTCAATGAT 22012 CACTTGAAATAATTCAATGAT baal40p01 ATAAAATAGCTGGTGGTTAAT 22013 ATAAAATAGCAGGTGGTTAAT baal40p01 GCTCCTCTCCCAACACGCCAT 22014 GCTCCTCTCCAAACACGCCAT baal40p01 CCATTTCCCACAGGAATAGAT 22015 CCATTTCCCATAGGAATAGAT baal40p01 TGGCCCAGACCATAGCGAGGG 22016 TGGCCCAGACAATAGCGAGGG baal40p01 TCTCACATGGATGCTGTCTTT 22017 TCTCACATGGCTGCTGICTIT kr61a0901 GTCTATAATCGTTTTTGATGT 22018 GTCTATAATCTTTTTTGATGT kr61a0901 TTTATTTTACCAAGTAACAAT 22019 TTTATTTTACGAAGTAACAAT kr61a0901 TATTAATATAGACCTCTATAA 22020 TATTAATATAAACCTCTATAA kr61a0901 CAGTTAGTAATCACGCCTGCA 22021 CAGTTAGTAACCACGCCTGCA bah44b17 AAGACAGTGCGTCACTCACTG 22027 AAGACAGTGC-TCACTCACTG 22028 basd2k11 ATATTGAGTCTGAGGTAACAA 22029 GAATTGAGTC-GAGGTAACAA 22030 bags23e20 TGAAATCCTCCTCTTGCATTT 22032 TGAAATCCTCGTCTTGCATTT bags33i10 AACCAAGAAACATTATTAGTG 22033 AACCAAGAAATATTATTAGTG bags35k06 GAGAAGGAGGTTTGCTGATGG 22034 GAGAAGGAGGGTTGCTGATGG

TABLE 28-15 Haruna Niio SEQ Clones H602 ID NO: bags35k06 CTAAGGAAAAACATACCTAGT 22035 CTAAGGAAAAGCATACCTAGT BaGS22M17 AACCACACAA-CGAAGGGCTA 22037 TACCACACAAGCGAAGGGCTA 22038 baak21j24 GAGAACAAACGAGCAGACAGG 22039 GAGAACAAACAAGCAGACAGG baal16o08 TCAGTTCATCGCTAGCATAGA 22042 TCAGTTCATCACTAGCATAGA baal16o08 CCTTTGTCACAGCAACCGGGT 22043 CCTTTGTCACGGCAACCGGGT baal27n7 TGGTGTTCACGGGCGCGCTGG 22044 TGGTGTTCACCGGCGCGCTGG baal27n7 CGATCTCCTCTAGCTTGGAGA 22045 CGATCTCCTCCAGCTTGGAGA baal27n7 TAACCTGCCCGGCGATCTCCT 22046 TAACCTGCCCCGCGATCTCCT baet31B1103 GCCTCCTGCAGTTGAGCCTGA 22047 GCCTCCTGCAATTGAGCCTGA baet31B1103 CTGACCAGCGCGTCCTGCGAC 22048 CTGACCAGCGAGTCCTGCGAC BaGS24K08 CAATAGGAAAACAGTAGGAAG 22051 CAATAGGAAAGCAGTAGGAAG BaGS24K08 TCACTGGAGGTTGGAGTGGAT 22052 TCACTGGAGGATGGAGTGGAT bah16c17 TCGTGCTTATCACATCAACCT 22053 TCGTGCTTATTACATCAACCT bah16c17 ACATCAACCTAGTATCGTGTG 22054 ACATCAACCTCGTATCGTGTG bah16c17 ACGGGAGATGATGGGATTCAC 22055 ACGGGAGATGCTGGGATTCAC bags28l21 CTACTCCTACACCAGTGCCTC 22056 CTACTCCTACGCCAGTGCCTC bags28l21 GCCTGTTAGCATTAGGCATGA 22057 GCCTGTTAGCGTTAGGCATGA bags28l21 CAAGTTGGGCAGCGGAGAGGC 22058 CAAGTTGGGCTGCGGAGAGGC bags28121 AGATTTACTTGGGACGCTTGC 22059 AGATTTACTTTGGACGCTTGC bah31m22 TGGATCATCAATTTCATCACA 22060 TGGATCATCAGTTTCATCACA bah31m22 CGGGGTCTTGTTCGGGGATGT 22061 CGGGGTCTTGCTCGGGGATGT bah31m22 GTAGAGCTCTTGCTGGAAGGA 22062 GTAGAGCTCTAGCTGGAAGGA bah31m22 GTCTACATGCAAAGAACCCTG 22063 GTCTACATGCGAAGAACCCTG baal30c17 GTTAACTGTGGCACGCGTCTG 22064 GTTAACTGTGACACGCGTCTG baak37n01 GTCGATCTCCTGCATACGGTT 22066 GTCGATCTCCCGCATACGGTT

TABLE 28-16 Haruna Niio SEQ Clones H602 ID NO: BaGS24O11 GAAAGTATGCAACAGGAGGAT 22067 GAAAGTATGCCACAGGAGGAT BaGS24O11 TGCCAGCTAAGAAAAATAACT 22068 TGCCAGCTAATAAAAATAACT BaGS24O11 AGGCAGAAAATGTGAATTGTA 22069 AGGCAGAAAACGTGAATTGTA bah50i23 ATTCCAAGTTCGCACCACACA 22073 ATTCCAAGTTTGCACCACACA bah50i23 AGGTAGCAGAAAAGAGGTGAT 22074 AGGTAGCAGATAAGAGGTGAT bah50i23 GCTTGCATGCTAATGCCATCG 22075 GCTTGCATGCCAATGCCATCG bah50i23 TTTGGAGAACCATGAGATTAA 22076 TTTGGAGAACTATGAGATTAA BaH56D06 TTTCCACTTGGGCACTTCTCG 22077 TTTCCACTTGAGCACTTCTCG BaAK39M05 GCTGTGAATGAGTTTCCAGCT 22078 GCTGTGAATGTGTTTCCAGCT BaAK39M05 TCCATCTGACCATGAGTTCCC 22079 TCCATCTGACCATGAGTTCCC bah11m16 TATATATACCGTGCTTGCTAT 22080 TATATATACCATGCTTGCTAT bah49d03 ACCCGAAGCATCGGTCGCATA 22081 ACCCGAAGCAACGGTCGCATA bah49d03 CCACCTCAAAGATGAATGACT 22082 CCACCTCAAAAATGAATGACT bah49d03 TTCCCCTGATTTCTGCGATCG 22083 TTCCCCTGATGTCTGCGATCG bah49d03 ATGAATGACTGGGTGGGATCA 22084 ATGAATGACTTGGTGGGATCA bah49d03 ACGTAGAACAGCGAATGACCA 22085 ACGTAGAACATCGAATGACCA bah49d03 GCAACCCGGTGCTTCAAGTCC 22086 GCAACCCGGTACTTCAAGTCC bah62l23 ATTTGCCTATGCAAGAAAGAT 22087 ATTTGCCTATACAAGAAAGAT bah62l23 CCACAATTGCGAATTTGCCTA 22088 CCACAATTGCGAATTTGCCTA BaAK38J13 TACTGACTGCGATGTACCGTC 22089 TACTGACTGCGATGTACCGTC BaAK38J13 AACAGGTTACGTTCAAGTTAC 22090 AACAGGTTACATTCAAGTTAC BaAK38J13 GCCAGATGCTTTGTAGGATTC 22091 GCCAGATGCTGTGTAGGATTC BaH37N04 GAGATAATGTAAACGCTAGAA 22092 GAGATAATGTGAACGCTAGAA BaH37N04 TGTGACCCAGTAAGAAGGGGC 22093 TGTGACCCAGAAAGAAGGGGC BaH37N04 ATTGTTGCATGAGTAAAAAGA 22094 ATTGTTGCATTAGTAAAAAGA

TABLE 28-17 Haruna Niio SEQ Clones: H602 ID NO: BaH37N04 GTACAACAGCGAAAAGTTAAC 22095 GTACAACAGCAAAAAGTTAAC basd27c06 GCCGAGGCGCCTGTGCCGTGC 22096 GCCGAGGCGCGTGTGCCGTGC bah39g10 TGGAGAATGTTCAAGACACCG 22097 TGGAGAATGTACAAGACACCG bah39g10 GATACGAAAAAGACGAGAATG 22098 GATACGAAAAGGACGAGAATG bags20a13 TGTCTCCAATTGTCTTCTCTA 22099 TGTCTCCGATGGTCTTCTCTA bags20a13 GATAAACGTACTTGCATTTAT 22100 GATAAACATAGTTGCATTTAT bags20a13 CACTGTCTCCAATTGTCTTCT 22101 CACTGTCTCCGATGGTCTTCT bags20a13 AAAGATAAACGTACTTGCATT 22102 AAAGATAAACATAGTTGCATT BaH27L15 CCGTTTCCTCGGAGATCGCAT 22104 CCGTTTCCTCAGAGATCGCAT bah59f07 CCAGACTCAGCGTCCCTACTG 22105 CCAGACTCAGTGTCCCTACTG bah59f07 TGGACAGCAACCCCAGACTCA 22106 TGGACAGCAAGCCCAGACTCA bags9l05 ATACAGAGAAACAAATGATAT 22107 ATACAGAGAAGCAAATGATAT bags9l05 GCCTCAGCAGGAGCTCCACCT 22108 GCCTCAGCAGCAGCTCCACCT bags9l05 CACCAGCAGCAGCACCACCAC 22109 CACCAGCAGCGGCACCACCAC bags9l05 CACCACCACCAATCGGACCAG 22110 CACCACCACCGATCGGACCAG bags9l05 CCACCTGCATTAAGTTTCAGA 22111 CCACCTGCATCAAGTTTCAGA bags9l05 GTAATGGTCCATTAGTTCAGC 22112 GTAATGGTCCCTTAGTTCAGC bah53j21 TTTCAAGCACCCTCTTGTTCA 22116 TTTCAAGCACGCTCTTGTTCA bah53j21 AATGTATGCCGAAATTTGATT 22117 AATGTATGCCAAAATTTGATT bah53j21 CCGCAGCAGAAATGATGAGCT 22118 CCGCAGCAGAGATGATGAGCT bah21f16 GATTCATAGGAATAGGAAAAA 22119 GATTCATAGGTATAGGAAAAA bah21f16 TAAAAACATAAGAACAAGAAA 22120 TAAAAACATAGGAACAAGAAA baet39E0309 TGCTTCAGGCTGATCGGTCGG 22121 TGCTTCAGGCAGATCGGTCGG baet39E0309 TTCGTTCTCCAACGGCGGATG 22122 TTCGTTCTCCAACGGCGGATG bags5l04 TCACATACCCGGCACCGGGAA 22123 TCACATACCCAGCACCGGGAA

TABLE 28-18 Haruna Niio SEQ Clones H602 ID NO: bags5l04 TCCGCCATTTTGTTCGAATGC 22124 TCCGCCATTTGGTTCGAATGC bags5l04 AGCACCCGCATCATTTAGCAG 22125 AGCACCCGCAGCATTTAGCAG bags7g10 TGTAGGTTTCCGCCTCATCAG 22126 TGTAGGTTTCTGCCTCATCAG bags7g10 AACAATACACAACCCTATTIG 22127 AACAATACACGACCCTATTTG bags9d22 ATATGTTCTCGACAGCCCGC- 22128 ATATGTTCTCGACAGCCCGCA 22129 bah18j14 GGAAAAAATTGCTTGTCAAAT 22132 GGAAAAAATTTCTTGTCAAAT bah18j14 TTCCCAACATAATTGCAATGT 22133 TTCCCAACATGATTGCAATGT bags37k14 GACCGAATGATAGATCTCATA 22134 GACCGAATGAGAGATCTCATA bags37k14 GCATTGATTTAATCTATGTTA 22135 GCATTGATTTGATCTATGTTA bah42k03 CATAGCCCGATGCCACGACAT 22136 CATAGCCCGACGCCACGACAT bah42k03 TAAAGGGCAGGGGAAAATATC 22137 TAAAGGGCAGGGGAAAATATC bags12i02 CGACTCTATGGGCAGCCACCG 22139 CGACTCTATGAGCAGCCACCG bags12i02 CCACGTAAGGCAAATCTCCTG 22140 CCACGTAAGGGAAATCTCCIG bags12i02 CAGACAGCAGCTCTTAGTCGA 22141 CAGACAGCAGTTCTTAGTCGA bags12i02 CACTATACAGATTGTAAAACC 22142 CACTATACAGTTTGTAAAACC bags12i02 ATGACGCCATAATATAAATCA 22143 ATGACGCCATTATATAAATCA baet25b0604 GCGCTTGCTCGATGGAGGATG 22145 GCGCTTGCTCAATGGAGGATG baet25b0604 TGCAGTAGTTATAGATCATGT 22146 TGCAGTAGTTGTAGATCATGT baak46e21 ACTAATCTACGAATATTTGCC 22147 ACTAATCTACGAATATTTGCC baak46e21 GCATTCTGTGGCCTAACTAAA 22148 GCATTCTGTGCCCTAACTAAA bags37j12 ATGGAGGGTTTGTTGGAGGCT 22149 ATGGAGGGTTCGTTGGAGGCT bags37j12 CATACACACTGCTTCATGCAT 22150 CATACACACTTCTTCATGCAT bags37j12 ATACGGCATGCGATTAAACAG 22151 ATACGGCATGTGATTAAACAG baak30b08 AAACAGTGTGCCCATCAGGCA 22153 AAACAGTGTGTCCATCAGGCA baak20o12 ATGCATCTAGGTTCTAGATAC 22155 ATGCATCTAGATTCTAGATAC _

TABLE 28-19 Haruna Niio SEQ Clones H602 ID NO: baak20o12 GCCTGATCGTCCTGGAGCGTC 22156 GCCTGATCGTGCTGGAGCGTC baak20o12 CCACGCTCCCGATGAAGAAGA 22157 CCACGCTCCCCATGAAGAAGA baak20o12 TAGATGCTAGCCATGCATCTA 22158 TAGATGCTAGTCATGCATCTA basd14k23 CTGCTCATGCTGAGAAGGCTG 22159 CTGCTCATGCCGAGAAGGCTG BaAK23L05 GCTTACCAGCGAAGATCAGGC 22160 GCTTACCAGCAAAGATCAGGC BaAK23L05 CCACCTCCAAGGTGATGGTCT 22161 CCACCTCCAAGGTGATGGTCT BaAK23L05 CACCACGAAGGCGGAGGACAA 22162 CACCACGAAGACGGAGGACAA BaAK23L05 TCGCCTTCACGTTGTCAATGG 22163 TCGCCTTCACATTGTCAATGG bags33o01 TGTGCTTGGATTTCAAGTTCA 22165 TGTGCTTGGACTTCAAGTTCA bags33o01 GGTATATATGCGAGTGTGCAA 22166 GGTATATATGTGAGTGTGCAA bah24d24 ACCCAATGGCGGGCAGTTTGG 22168 ACCCAATGGCAGGCAGTTTGG bah24d24 TGCAGGGAATTCTCAGCAAGG 22169 TGCAGGGAATCCTCAGCAAGG bags12f09 CCACTCGTAGTAGCGTGTGTT 22170 CCACTCGTAGCAGCGTGTGTT baak33h23 ACGATCATATGGCACTTATAT 22171 ACGATCATATTGCACTTATAT baak33h23 TTTTACAAGCAACATAAGGAA 22172 TTTTACAAGCGACATAAGGAA baak33h23 CTTGTTCACAGGAAGTCTACT 22173 CTTGTTCACACGAAGTCTACT bah47p03 TTTTTAGGACACTCATAGCCT 22174 TTTTTAGGACGCTCATAGCCT bah47p03 AAAATCAGAAGGAGAATTTGA 22175 AAAATCAGAAAGAGAATTTGA bah47p03 CTGACTAATCTTCCGGGGTAG 22176 CTGACTAATCGTCCGGGGTAG basd14f09 GCTTACCAGCAAAGATCAGGC 22177 GCTTACCAGCGAAGATCAGGC BaSD22F10 GCAAAGATTAGGCGCTGCTGG 22179 GCAAAGATTAAGCGCTGCTGG BaSD22F10 TCAACCTCCAGTGTGATGGTC 22180 TCAACCTCCAATGTGATGGTC BaSD22F10 GCTGGTCCGGGGGGATGCCCT 22181 GCTGGTCCGGAGGGATGCCCT BaSD22F10 TGGTCTTGCCGGTGAGAGTCT 22182 TGGTCTTGCCGGTGAGAGTCT BaSD22F10 GCTTACCAGCAAAGATCAGGC 22183 GCTTACCAGCGAAGATCAGGC_

TABLE 28-20 Haruna Niio SEQ Clones H602 ID NO: bags27o20 GTTGATCTAAAGGCAGATCCA 22186 GTTGATCTAAGGGCAGATCCA bags27o20 GGTCGTCTGACGGAGGACTGT 22187 GGTCGTCTGATGGAGGACTGT bags27o20 CTCTTTCGGGTTCATCGCCAG 22188 CTCTTTCGGGGTCATCGCCAG baak31o10 AATCAGCACTCAACTGACCTT 22189 AATCAGCACTGAACTGACCTT baak31o10 GAAATCGTGATGCCTAACGCC 22190 GAAATCGTGAGGCCTAACGCC bags10f16 CAAAGGACTCAATGACGGGGA 22191 CAAAGGACTCGATGACGGGGA bags10f16 TGCTTGAGAATCCAAAGGACT 22192 TGCTTGAGAACCCAAAGGACT BaGS9H02 GTCGGCAACCGCATTTCCCTT 22194 GTCGGCAACCACATTTCCCTT kr66C0705 GTGTGCACATCGTCTGATCAG 22197 GTGTGCACATTGTCTGATCAG kr66C0705 CTTTTGGGAATGTTAACTGAT 22198 CTTTTGGGAACGTTAACTGAT kr66C0705 GGTGATCCGAACGTGTGAATA 22199 GGTGATCCGAGCGTGTGAATA kr66C0705 CTTTGATCTGGGCAGTAGTCG 22200 CTTTGATCTGTGCAGTAGTCG BaGS33M05 CCATGCCCTCCCCTGTGTACC 22201 CCATGCCCTCGCCTGTGTACC BaGS33M05 CTATCACCACGTGACAGCACC 22202 CTATCACCACCTCACAGCACC baal24b02 CCTAGAGCATAATATGCGGGT 22208 CCTAGAGCATGATATGCGGGT baal24b02 CTCCATGCCTAAACCAGCAAC 22209 CTCCATGCCTGAACCAGCAAC bags34f08 CAGATATATCTTTGCCAAATC 22210 CAGATATATCATTGCCAAATC bags34f08 CAGTGTTTCAAACTATTGTTA 22211 CAGTGTTTCAGACTATTGTTA bags34f08 GGCTTATTTTGATCTGCCACA 22212 GGCTTATTTTCATCTGCCACA bast36D1208 GCCGTCGCTACCCAAGCTCCC 22213 GCCGTCGCTATCCAAGCTCCC kr25b1103 ACTGCCCACCGGTCGTCCCCA 22214 ACTGCCCACCAGTCGTCCCCA kr25b1103 AGGGCTGCGGCCCCTACTGCC 22215 AGGGCTGCGGTCCCTACTGCC kr25b1103 GATCAACACGCTGAAGCTGGG 22216 GATCAACACGTTGAAGCTGGG baal19g05 ACACCAGCTGGCTGACTGACT 22217 ACACCAGCTGACTGACTGACT baal19g05 TCTACATACAGTTCGCTAACC 22218 TCTACATACAATTCGCTAACC

TABLE 28-21 Haruna Niio SEQ Clones H602 ID NO: baal19g05 GATGCCCGTCGGAGTCAGGCG 22219 GATGCCCGTCAGAGTCAGGCG baal19g05 TTCGCTAACCGCAAGAACAAG 22220 TTCGCTAACCACAAGAACAAG bags19c10 ACGCAAAAAAAAGAGTAATAA 22222 ACGCAAAAAAGAGAGTAATAA bags19c10 TACAACAGTGTATAAGAAGGC 22223 TACAACAGTGCATAAGAAGGC bags19c10 TTCTTCTTAGGGGCTAAATGA 22224 TTCTTCTTAGCGGCTAAATGA bags19c10 ACTTTAAGCATATGTGCTTAG 22225 ACTTTAAGCAGATGTGCTTAG bags19c10 TGCAAGCCATCATAGATCTAT 22226 TGCAAGCCATTATAGATCTAT bags29k01 CTCATAAAGTTGACATTCAGG 22227 CTCATAAAGTGGACATTCAGG bags29k01 AAGCTTAAGAAAGACAAACTC 22228 AAGCTTAAGACAGACAAACTC bags29k01 CTCCTCATCAATCCCAATGGC 22229 CTCCTCATCAGTCCCAATGGC bags29k01 CAGGAGATGTTACAAACAAAT 22230 CAGGAGATGTGACAAACAAAT bags29k01 TCATCGTCCCGAGAATCGATC 22231 TCATCGTCCCAAGAATCGATC bags29k01 ACTTGCCGGCAGAGTATCAGG 22232 ACTTGCCGGCGGAGTATCAGG bags29k01 TGAGTGCTTCTTTCTCAGGCT 22233 TGAGTGCTTCCTTCTCAGGCT bags29k01 GGCTAACATCGACACTGAAAG 22234 GGCTAACATCTACACTGAAAG bags29k01 TGAAAGGCCCAACAAGTGTGA 22235 TGAAAGGCCCGACAAGTGTGA bags29k01 AAACAAATCAACCTACATTTC 22236 AAACAAATCATCCTACATTTC bastl54G0414 TATCTAGAAGACCAGAACATA 22237 TATCTAGAAGGCCAGAACATA bastl5400414 CTACTCCTTCGGACATTCCCC 22238 CTACTCCTTCCGACATTCCCC bags34b15 AGCCCCTTGT-CAGACCGCTG 22240 AGCCCCTTGTACAGACCGCTG 22241 bah31l17 AAAATAATAAGAGGAACCCAC 22244 AAAATAATAAAAGGAACCCAC bah31l17 TTGATCAAATAAGGCCAAAAT 22245 TTGATCAAATGAGGCCAAAAT bah31l17 AGTCAAACTGATTGCACATTT 22246 AGTCAAACTGGTTGCACATTT bah31l17 ATCAAAAACAGTATAGAACTT 22247 ATCAAAAACATTATAGAACTT bah31l17 AGGATGCCACAGCCTTTTGTA 22248 AGGATGCCACGGCCTTTTGTA _

TABLE 28-22 Haruna Niio SEQ Clones H602 ID NO: baak32a05 GACCATGCACAATCTCTTCCA 22249 GACCATGCACTATCTCTTCCA baak32a05 TCCCCTCCTTTCCCACAGTCA 22250 TCCCCTCCTTGCCCACAGTCA bags30n22 CACCTAATGTAGATGGAGCCA 22251 CACCTAATGTTGATGGAGCCA bags30n22 CACTGGCATTCTTGCCATGGT 22252 CACTGGCATTITTGCCATGGT bags30n22 ACTCTTATCGTCAGTAGCTGA 22253 ACTCTTATCGGCAGTAGCTGA BaH27L17 AGCCCTCGACGCCGATGATGT 22258 AGCCCTCGACTCCGATGATGT BaH27L17 TCGAGCAATCGAGCGCGATGA 22259 TCGAGCAATCAAGCGCGATGA BaH27L17 TTCTGTTTGCGGGTGTGTTGA 22260 TTCTGTTTGCTGGTGTGTTGA BaH27L17 GCAACTCTATGCCCCAACGAA 22261 GCAACTCTATTCCCCAACGAA basd17e11 ATATGGTCTCGAAGAGTTCCT 22262 ATATGGTCTCAAAGAGTTCCT basd17e11 TGTATTAAGAAGTGCATGCTA 22263 TGTATTAAGAGGTGCATGCTA basdl7e11 GGTACTCCTGGCCACTGTTTA 22264 GGTACTCCTGTCCACTGTTTA basd17e11 ACTTCGGTCGGTGICCTGCAA 22265 ACTTCGGTCGCTGTCCTGCAA basd17e11 TGTCCTGCAACCCCAAAGGAC 22266 TGTCCTGCAAACCCAAAGGAC bags15i06 TCGAACTCCTCTGGTCGCTAG 22267 TCGAACTCCTITGGTCGCTAG bags15i06 ACTGCAGGACAAGCTGCTTCA 22268 ACTGCAGGACGAGCTGCTTCA bags15i06 CTGCTCCTGGTACTGCAGGAC 22269 CTGCTCCTGGGACTGCAGGAC bags15i06 AGTAGATTTTTCAGTTCAGAT 22270 AGTAGATTTTGCAGTTCAGAT bags15i06 CAGCGCGTAGTTGTTGATCTG 22271 CAGCGCGTAGGTGTTGATCTG bags15i06 CGCCCTATCCCACGGCCAACA 22272 CGCCCTATCCGACGGCCAACA bags15i06 ACTCTCAATCACCAAGGGCCA 22273 ACTCTCAATCTCCAAGGGCCA baak36f09 CGATGGCGGCTCCGTAGTGCG 22274 CGATGGCGGCGCCGTAGTGCG baak36f09 CGGGCACGCGTGTGAGGTAGA 22275 CGGGCACGCGGGTGAGGTAGA bast57a0101 TTCTCCATTACAAACCTATTA 22276 TTCTCCATTAIAAACCTATTA basd13b15 GGTGAGGCTCGCGTCTTCGCT 22278 GGTGAGGCTCACGTCTTCGCT

TABLE 28-23 Haruna Niio SEQ Clones H602 ID NO: basd13b15 TCAGCTGCGCGTTGTCGATGA 22279 TCAGCTGCGCATTGTCGATGA bags9d11 ACAATCATCAGATATGAGTGG 22280 ACAATCATCATATATGAGTGG bags9d11 GGGCCCATAGTTAACCCAACT 22281 GGGCCCATAGGTAACCCAACT bags9d11 CTAAGTTGTTGTATAGGCATC 22282 CTAAGTTGTTGTATAGGCATC bags9d11 TAACCCAACTTCTTGGGGGAA 22283 TAACCCAACTCCTTGGGGGAA bags9d11 TGTTCCATATTTGATCGATGA 22284 TGTTCCATATCTGATCGATGA bags9d11 GTTTTGCCGCGCGATATAGTG 22285 GTTTTGCCGCACGATATAGTG bags9d11 GTAATAAATTGAATGCTCTTC 22286 GTAATAAATTAAATGCTCTTC bags9d11 TGTCTTAATTGTTAGTCTTCA 22287 TGTCTTAATTATTAGTCTTCA bags9d11 AAACTAAAACTTACCTGTGGG 22288 AAACTAAAACATACCTGTGGG bags9d11 ATTTCAGCCTGTTAAAAAACC 22289 ATTTCAGCCTATTAAAAAACC kr30f1212 TTCGACAAGAGAGCCACAAAC 22290 TTCGACAAGAAAGCCACAAAC bastl45b0903 CAGTCCAACACTTCTTTCTAA 22291 CAGTCCAACATTTCTTTCTAA BaGS32I12 CCTTGGCCGCCGCAGATGCGT 22292 CCTTGGCCGCTGCAGATGCGT BaGS32I12 CACAGCCATCGTATGGCATGT 22293 CACAGCCATCITATGGCATGT BaGS32I12 CCGAGCAAACTACATTTTGCT 22294 CCGAGCAAACCACATTTTGCT baak27p06 AGTGACAATGCCATGCTTTTG 22296 AGTGACAATGTCATGCTTTTG baak27p06 CAAGAAAAATTTCCTAAAAGG 22297 CAAGAAAAATGTCCTAAAAGG baak27p06 CAAGTACCTCAGTAAAATCAT 22298 CAAGTACCTCGGTAAAATCAT baak27p06 CATCACCAAGCAGGGGGTTCC 22299 CATCACCAAGTAGGGGATTCC baak27p06 CTGGGTCTCAAAAGTGACAAT 22300 CTGGGTCTCAGAAGTGACAAT bags21m21 TCCCCTGGAAAACCTCGCCGC 22301 TCCCCTGGAAGACCTCGCCGC basd16e11 TAGGTTCATACATTCACGTAT 22304 TAGGTTCATACATTCACGTAT basd16e11 TGCTCGCGCACCTCTCCACCA 22305 TGCTCGCGCATCTCTCCACCA basd16e11 TCAAAGAACACAGGAAGGATG 22306 TCAAAGAACAGAGGAAGGATG _

TABLE 28-24 Haruna Niio SEQ Clones H602 ID NO: basd16e11 CAGTCTCTATTCTTTGATCCA 22307 CAGTCTCTATGCTTTGATCCA baak21n09 ATCCATAACTTTTGGCCACCC 22308 ATCCATAACTGTTGGCCACCC baak21n09 CCACTTCCTCATCCGCATTTG 22309 CCACTTCCTCGTCCGCATTTG baak21n09 CTTGCAAGCTATCGAACTGGC 22310 CTTGCAAGCTGICGAACTGGC baak1g17 ACATGAAACAAACTCAGACCT 22311 ACATGAAACAGACTCAGACCT baak1g17 AACAGAGCACTGTTCGTTTCA 22312 AACAGAGCACCGTTCGTTTCA baak1g17 GTGGTTTTTTCCGCTACTTCA 22313 GTGGTTTTTTGCGCTACTTCA baal35f12 AGCTTATTATGCTCATGTTCA 22315 AGCTTATTATTCTCATGTTCA baal35f12 AGTTGGATACNAGAGACACTA 22316 AGTTGGATACAAGAGACAGTA baal35f12 GACCACCTCTGCATGATAGAG 22317 GACCACCTCTACATGATAGAG bah29b02 ACTTCTTGCCATCAGTGCTTG 22320 ACTTCTTGCCGTCAGTGCTTG bah29b02 TTAAGCCAAGTTCAGTACTGG 22321 TTAAGCCAAGGTCAGTACTGG bah29b02 ATCTGCTGACTGATTCACCAA 22322 ATCTGCTGACCGATTCACCAA bah29b02 AGCATACCAAAATTTTTAGGT 22323 AGCATACCAAGATTTTTAGGT kr61C0305 CCGGGCAAAAGCATGTTGTTT 22325 CCGGGCAAAAACATGTTGTTT kr61C0305 ATAATATGCAAGAACACTTGT 22326 ATAATATGCAGGAACACTTGT kr61C0305 CCTTTGATATATCTGCATGCA 22327 CCTTTGATATGTCTGCATGCA BaH23B08 ACAATCCAAGGCACCTTCATA 22328 ACAATCCAAGACACCTTCATA bah51g17 CAAATCAATTACACAAATAGT 22330 CAAATCAATTACACAAATAGT bah51g17 TAGAGAGCTGCAACGCCACAT 22331 TAGAGAGCTGAAACGCCACAT bah51g17 CATGTTAAAATTTGTAGGGAA 22332 CATGTTAAAACTTGTAGGGAA basd22e19 TGCCGGGCGCTTAGCAGCAGC 22333 TGCCGGGCGCATAGCAGCAGC basd22e19 CTTGCATATTGTGTCGGAGCC 22334 CTTGCATATTATGTCGGAGCC basd22e19 TATTAGTCCATAAGACAATAC 22335 TATTAGTCCACAAGACAATAC basd22e19 TAATATGATTAAAGTTTTGAG 22336 TAATATGATTGAAGTTTTGAG

TABLE 28-25 Haruna Niio SEQ Clones H602 ID NO: basd22e19 GAGAATATAAAATGTATGTTT 22337 GAGAATATAAGATGTATGTTT basd22e19 CCATTTCTTCAATCTTAGCAA 22338 CCATTTCTTCCATCTTAGCAA basd22e19 TAGCAGTGCAGGCATATCATT 22339 TAGCAGTGCAGGCATATCATT basd22e19 ATTGTAGGTACACATTAACAT 22340 ATTGTAGGTAAACATTAACAT basd22e19 CATTCAACAACTTATTGTAGG 22341 CATTCAACAATTTATTGTAGG basd22e19 TTTTGCATGGAAAGAGAATAT 22342 TTTTGCATGGGAAGAGAATAT

As described above, a gene polymorphism detection instrument according to the present invention is realized with a support immobilized thereon polynucleotides that comprise part of the base sequences of amplified DNA fragments that have polymorphism between different varieties. The amplified DNA fragments with polymorphism are not limited to those shown in Table 8-1 through Table 14-9, as long as they are DNA fragments that have polymorphism between different varieties and that have been amplified, using the genomic DNA of Triticeae species as a template, with a primer set that comprises a combination of two primers arbitrarily selected from (i) primers that have been designed based on the base sequence of SEQ ID NO: n (where n is an odd number), and (ii) primers that have been designed based on the base sequence of SEQ ID NO: (n+1), from among the base sequences of SEQ ID NO: 1 through 5780. In the case of SNP, part of amplified DNA fragments is a polynucleotide that comprises the base sequence of a region including a SNP base. In fragment length polymorphism, part of amplified DNA fragments is a polynucleotide that includes, or does not include, the base sequence of a region, if specified, causing a fragment length difference (a base sequence portion that is present in one of the varieties but not in the other). A polynucleotide that includes such base sequence portion, and a polynucleotide that does not include such base sequence portion may be used together.

In the case where a gene polymorphism detection instrument according to the present invention is used for detection of single nucleotide polymorphism (SNP), the polynucleotide immobilized on the support is preferably a synthetic oligonucleotide. Many SNP detecting arrays currently available use synthetic oligonucleotides, and these techniques can be used for the present invention. The polynucleotide immobilized on the support may have any number of bases as long as it can detect gene expression. For example, in the case where only one synthetic oligonucleotide is immobilized in each region, an oligonucleotide with at least 50 bases is considered to be sufficient for detection of gene expression. When more than one synthetic nucleotide is immobilized in each region as in the Affymetrix system, an oligonucleotide with about 25 bases is sufficient.

[Gene Polymorphism Detection Instrument with the Polynucleotides Immobilized in Regions that are Arranged in the Chromosomal Order]

As described above, the chromosomal order of polynucleotides on barley chromosomes (1H, 2H, 3H, 4H, 5H, 6H, or 7H) (distance from the short arm end of each chromosome) has been specified in clones that have the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1 through SEQ ID NO: 5780. Thus, if the polynucleotides comprising part of the DNA fragments amplified with the primers that have been designed based on the respective base sequences were placed, for example, according to the order of the polynucleotides, as represented in Table 1-1 through Table 7-9, having the reference base sequences of the primers, it would be possible to place the polynucleotides according to the order in which these polynucleotides are arranged from the short arm end of 1H chromosome to the long arm end of 7H chromosome, i.e., chromosomal order. For clones that have the same order from the short arm end, the order by which these clones are placed is not particularly limited. Once the precise order of these clones were specified by a future study, these clones will be able to be placed accordingly. Note that, the reference point (origin) on the chromosome is not just limited to the short arm end of 1H chromosome. Any position on a chromosome can be used as a reference point. If the polynucleotides immobilized on the support have overlapping portions, one closest to the 5′ end is placed first. When there is more than one polynucleotide having the same 5′ end position, these polynucleotides are placed contiguously in any order relative to one another.

With the polynucleotides immobilized on a support in the order they are arranged on the chromosomes, the value of a gene polymorphism detection instrument according to the present invention can be greatly improved in crossbreeding of Triticeae species. More specifically, by using a gene polymorphism detection instrument according to the present invention for comprehensive investigation of gene polymorphism of Triticeae species, the genotype of a target gene of breeding can be determined. Further, the location and extent of recombination on chromosomes can be checked to see if unnecessary recombination has occurred. That is, the genotypes of the parents are directly inherited as the haplotype in portions of the chromosomes where no recombination has occurred. As a result, the genotype of a trait conferred to the selected individuals can be easily determined, with the result that the efficiency of breeding is improved.

[Gene Polymorphism Detection Instrument with the Polynucleotides Immobilized in Regions appended with Chromosomal Order Information]

The regions in which the polynucleotides are immobilized (spots) may be appended with information indicative of the order in which the polynucleotides are arranged on barley chromosomes. The spots may be arranged in any way as long as they are appended with the order information. With the order information added to the spots, the data obtained from the spots can be rearranged in the chromosomal order even when the spots are randomly placed on a support. In this way, a gene detection instrument according to the present invention can improve the efficiency of breeding. Note that, the order information added to the spots can follow the foregoing criteria used to place the polynucleotides.

Adding order information enables the spots to be arranged in an arbitrary order in a gene polymorphism detection instrument of an array type, in which more than one polynucleotide is immobilized on a support such as a membrane or a glass slide. Further, the chromosomal order information of individual polynucleotides can also be added in a gene polymorphism detection instrument that employs a collection of beads (bead array) in which the polynucleotide is immobilized on each bead serving as a support.

[Examples of a Gene Polymorphism Detection Instrument according to the Present Invention]

Various conventional techniques that are designed for detection of gene polymorphism, particularly single nucleotide polymorphism (SNP) can be suitably used for a gene polymorphism detection instrument according to the present invention.

A representative example of such conventional techniques is GeneChip array for DNA analysis (Affymetrix). In this array, total genomic DNA is excised with restriction enzyme, and adapters that recognize cohesive ends of 4 bases are ligated. The adapters can be ligated to all fragments of any size generated by the restriction enzyme treatment. From these fragments, fragments of 250 to 1000 bp are selectively amplified by PCR and hybridized with an array that has been designed to cause a match or mismatch with SNP portions. Many other conventional SNP detecting arrays have been commercially marketed, which are also applicable to a gene polymorphism detection instrument according to the present invention. The present invention can also employ the techniques disclosed in Non-Patent Publications 1 and 2.

Alternatively, an array system can be realized with use of Gene Silicon of TOYO KOHAN. Gene Silicon is a multi-purpose chip made of a DLC (diamond like carbon)-coated semiconductor silicon substrate with an activated ester-bearing carboxyl group introduced on the surface. With the activated ester-bearing carboxyl group, Gene Silicon can immobilize DNA or proteins on the substrate.

For example, a plurality of primer sets is designed that serves as genetic markers for distinguishing barley variety A and barley variety B, and that can cause amplification in only one of the varieties when PCR is performed with the genomic DNA of these two varieties as templates. Specifically, the primers are preferably designed based on polymorphic sites of the both varieties.

One of the primers from each primer set is placed and immobilized on Gene Silicon in the chromosomal order. The array is then hybridized with genomic DNA prepared from the hybrid of a cross between variety A and variety B, and PCR reaction is performed in a reaction solution containing Cydye-dNTP.

The presence or absence of amplification in each spot can easily be confirmed by observing fluorescence emitted by the incorporation of Cydye-dNTP. Specifically, whether the genomic DNA that resides in portions of chromosomes where the genetic markers (amplified fragments) are mapped originates in which of the parents (variety A or variety B) is confirmed according to the presence or absence of amplification. In this way, locations of recombination can be found easily.

(3) Polypeptide-Interacting Substance Detection Instrument According to the Present Invention

A polypeptide-interacting substance detection instrument according to the present invention is an instrument for detecting substances that interact with proteins encoded by genes that reside in the genomes of Triticeae species, or substances that interact with polypeptides that constitutes part of the proteins. The organisms to which a polypeptide-interacting substance detection instrument of the invention is applicable may be any Triticeae species, among which barley, wheat, and rye are preferable. As will be described later, a polypeptide-interacting substance detection instrument according to the present invention includes a support on which polypeptides encoded by polynucleotides that constitute part of DNA of barley chromosomes (1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes) are immobilized. The polypeptides immobilized on the support may solely be polypeptides encoded by polynucleotides constituting part of barley chromosomal DNA, or other polypeptides may additionally be immobilized on the support. Such additional polypeptides are not particularly limited. For example, the additional polypeptides may be those encoded by polynucleotides with the base sequences originating in non-barley organisms, or those with arbitrary amino acid sequences that have been artificially synthesized.

In the case where the polypeptides are immobilized in more than one region of the support, the polypeptides immobilized in these regions may have non-overlapping amino acid sequences or partially overlapping amino acid sequences. Alternatively, polypeptides of the same amino acid sequence may be immobilized in these different regions of the support. In the case where the polypeptides have overlapping base sequences, the polypeptides may have partially overlapping amino acid sequences, or the amino acid sequence of one of the polypeptides may be a partial sequence of the other polypeptide.

Further, the polypeptide immobilized in each region is not necessarily required to be of the same kind. More than one kind of polypeptide may be immobilized in each region.

The support is not particularly limited as long as it can immobilize polypeptides, and it may have any shape and may be made of any material. Examples of a support material generally include: inorganic materials such as glass and silicon wafer; natural polymers such as paper; synthetic polymers such as nitrocellulose and nylon; and gels using synthetic polymers or natural polymers. The shape of the support is not particularly limited as long as it provides enough area to support the polypeptides. Generally, those with a two-dimensional plane, for example, such as a substrate with little or no flexibility, a flexible membrane, or a flexible substrate with intermediate flexibility can be preferably used. The thickness of the substrate or membrane is not particularly limited either, and it can be suitably set according to the material or use of the substrate or membrane. Various types of beads may be used as supports.

[Polypeptides Immobilized on a Support of the Polypeptide-Interacting Substance Detection Instrument]

In a polypeptide-interacting substance detection instrument according to the present invention, at least one polypeptide encoded by the following polynucleotides (a) or (b) is immobilized on a support.

(a) Polynucleotides with base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

(b) Polynucleotides with a combination of base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

As used herein, a polynucleotide with a base sequence constituting part of barley chromosomal DNA is not particularly limited as long as it is a polynucleotide with a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes, and including a protein-coding region. Further, a polynucleotide with a combination of base sequences constituting part of barley chromosomal DNA refers to a polynucleotide in which a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes is joined to non-continuous base sequences constituting other parts of these chromosomes, and in which a protein-coding region is contained. For example, base sequences from two different parts of the chromosomes may constitute the polynucleotide, or three or more base sequences may join together to form the polynucleotide. Specifically, for example, cDNA with a plurality of exons from a protein-coding gene on barley chromosomal DNA can be regarded as a polynucleotide with a combination of base sequences constituting part of barley chromosomal DNA. However, the polynucleotide is not just limited to this specific example.

A variant with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotide with a base sequence, or a combination of base sequences, constituting part of barley chromosomal DNA may be a polynucleotide that has been mutated on purpose, or a polynucleotide that exists in nature. For example, think of a base sequence of chromosomal DNA in a specific variety of barley. Comparing this base sequence with those of other varieties, no sequence is completely identical. Rather, these sequences are variants with the substitution, deletion, insertion, and/or addition of one or more bases. The polypeptide encoded by a polynucleotide with such variant base sequence may have the same amino acid sequence as the polypeptide encoded by a polynucleotide with a non-variant base sequence. Further, the polypeptides encoded by the polynucleotides with variant and non-variant base sequences may differ from each other with the substitution of some of the amino acids, or most of or all of the amino acid sequences may be different between these polypeptides.

Polynucleotides that encode polypeptides immobilized on a support of a polypeptide-interacting substance detection instrument according to the present invention are preferably polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, or variants with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotides with the base sequences of SEQ ID NO: 1 through 5780. (Such polynucleotides and variants will be referred to as polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780.)

The base sequences of SEQ ID NO: 1 through 5780 are base sequences of the barley EST (expressed sequence tag) independently developed by the inventors. The inventors have previously confirmed that a polynucleotide with the base sequences of SEQ ID NO: 1 through 770, a polynucleotide with the base sequences of SEQ ID NO: 771 through 1754, a polynucleotide with the base sequences of SEQ ID NO: 1755 through 2642, a polynucleotide with the base sequences of SEQ ID NO: 2643 through 3324, a polynucleotide with the base sequences of SEQ ID NO: 3325 through 4320, a polynucleotide with the base sequences of SEQ ID NO: 4321 through 4962, and a polynucleotide with the base sequences of SEQ ID NO: 4963 through 5780 are mapped on 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes, respectively.

It follows from this that the base sequences of SEQ ID NO: 1 through 5780 are partial sequences of barley cDNA. Thus, if polynucleotides with the base sequences of SEQ ID NO: 1 through 5780 contained protein-coding regions, polypeptides encoded by these polynucleotides can be immobilized on the support. Further, variants with the substitution, deletion, insertion, and/or addition of one or more bases in the base sequences of SEQ ID NO: 1 through 5780 constitute variant base sequences of barley cDNA. Thus, if these polynucleotides with variant base sequences contained protein-coding regions, polypeptides encoded by these polynucleotides can be immobilized on the support. The polynucleotides with variant base sequences may be polynucleotides that have been mutated on purpose, or polynucleotides that exist in nature.

Polynucleotides that encode polypeptides immobilized on a support of a polypeptide-interacting substance detection instrument according to the present invention may be a part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780. Since the base sequence of such partial polynucleotide is a partial base sequence of barley cDNA, a polypeptide encoded by such partial polynucleotide can be immobilized on the support if the partial polynucleotide contains a protein-coding region.

Further, polynucleotides that encode polypeptides immobilized on a support of a polypeptide-interacting substance detection instrument according to the present invention may be polynucleotides whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. The remaining base sequences of the polynucleotides are not limited. For example, since the base sequences of SEQ ID NO: 1 through 5780 are partial sequences of barley cDNA, these base sequences do not have the sequences on either end as originally found in the full length cDNA. Thus, a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780, and which additionally includes the cDNA sequences on the both ends or one end as originally found in the full length cDNA can be regarded as a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. Further, vectors such as plasmids and BACs (bacterial artificial chromosomes) that have incorporated all of or part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780, and polynucleotides in which the partial sequence is ligated to arbitrary base sequences can also be regarded as polynucleotides whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. Such polynucleotides at least include all of or part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780, i.e., part of barley cDNA. Therefore, polypeptides encoded by such polynucleotides can be immobilized on the support if the polynucleotides contain a protein-encoding region.

Further, polynucleotides that encode polypeptides immobilized on a support of a polypeptide-interacting substance detection instrument according to the present invention may be polynucleotides or the like whose partial sequences comprise all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n (where n is an odd number), and all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780. As described above, the base sequences of SEQ ID NO: 1 through 5780 are EST sequences of barley, and comprise sequences that can be read by sequencing the cloned cDNA from the both ends only once. In other words, the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 among the base sequences of SEQ ID NO: 1 through 5780 are base sequences that are read from the both ends of the cDNA of the same clone. As such, these base sequences can realize a full length cDNA base sequence, which corresponds to all of or part of the full length cDNA. Thus, polynucleotides or the like whose partial sequences comprise all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n (where n is an odd number), and all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780 can be regarded as polynucleotides with full length cDNA that comprises the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1, or polynucleotides that comprise part of the full length cDNA. Further, the polynucleotides may be polynucleotides in which vector sequences or arbitrary base sequences for example are ligated to the both ends or one end of the full length cDNA or a polynucleotide that comprises part of the full length cDNA. Further, the polynucleotides may be variants that have a base substitution or other mutations in sequences other than the base sequences of SEQ ID NO: 1 through 5780, i.e., a middle section of the total cDNA unspecified by SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1. Such polynucleotides include the full length or part of barley cDNA, enabling polypeptides encoded by the polynucleotides to be immobilized on the support. In particular, when the polynucleotides are full length cDNA or include full length cDNA, the entire proteins encoded by the genes located on the barley chromosomes can be immobilized on the support.

[Peptide-Interacting Substance Detection Instrument with the Polypeptides Immobilized in Regions that are Arranged in the Chromosomal Order]

As described above, the chromosomal order of polynucleotides on barley chromosomes (1H, 2H, 3H, 4H, 5H, 6H, or 7H) (distance from the short arm end of chromosome) has been specified in clones that have the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1 through SEQ ID NO: 5780. Thus, if the polypeptides encoded by the polynucleotides with the base sequences of SEQ ID NO: 1 through SEQ ID NO: 5780 were placed according to the order, as represented in Table 1-1 through Table 7-9, of the polynucleotides encoding the polypeptides, it would be possible to place the polypeptides according to the order from the short arm end of 1H chromosome to the long arm end of 7H chromosome, i.e., chromosomal order. For clones that have the same order from the short arm end, the order by which these clones are placed is not particularly limited. Once the precise order of these clones were specified by a future study, these clones will be able to be placed accordingly. Note that, the reference point (origin) on the chromosome is not just limited to the short arm end of 1H chromosome. Any position on a chromosome can be used as a reference point.

With the polypeptides immobilized on a support in the order they are arranged on the chromosomes, the location and extent of recombination on chromosomes can be checked for genes from which the expressed proteins originate. That is, the genotypes of the parents are directly inherited as the haplotype in portions of the chromosomes where no recombination has occurred. As a result, the genotype of a trait conferred to the selected individuals can be easily determined, with the result that the efficiency of breeding is improved.

[Polypeptide-Interacting Substance Detection Instrument with the Polypeptides Immobilized in Regions Appended with Chromosomal Order Information]

The regions in which the polypeptides are immobilized (spots) may be appended with information indicative of the order in which the polynucleotides encoding the polypeptides are arranged on barley chromosomes. The spots may be arranged in any way as long as they are appended with the order information. With the order information added to the spots, the data obtained from the spots can be rearranged in the chromosomal order even when the spots are randomly placed on a support. Note that, the order information added to the spots can follow the foregoing criteria used to place the polypeptides.

Adding order information enables the spots to be arranged in an arbitrary order in a polypeptide-interacting substance detection instrument of an array type, in which more than one polypeptide is immobilized on a support such as a membrane or a glass slide. Further, the chromosomal order information of individual polypeptides can also be added in a polypeptide-interacting substance detection instrument that employs a collection of beads (bead array) in which the polypeptide is immobilized on each bead serving as a support.

[Examples of a Polypeptide-Interacting Substance Detection Instrument According to the Present Invention]

Various conventional techniques that are designed for detection of protein (polypeptide)-interacting substances can suitably be used in a polypeptide-interacting substance detecting instrument according to the present invention.

For example, a biological chip, one kind of protein chip, has been commercially available from Ciphergen Biosystems, Inc. This biological chip has an activated group such as carbonyl diimidazole or epoxy on a surface of a substrate (support), allowing a user to freely immobilize target proteins or antibodies. This technique is therefore applicable to a polypeptide-interacting substance according to the present invention. Since the activated group such as carbonyl diimidazole or epoxy can immobilize antibodies or polynucleotides, the biological chip can be used for the fabrication of other detection instruments of the present invention.

(4) Polypeptide Detection Instrument according to the Present Invention

A polypeptide detection instrument according to the present invention is an instrument for detecting proteins encoded by genes that reside in the genomes of Triticeae species, or polypeptides that constitutes part of the proteins. The organisms to which a polypeptide detection instrument of the invention is applicable may be any Triticeae species, among which barley, wheat, and rye are preferable. As will be described later, a polypeptide detection instrument according to the present invention includes a support on which polypeptides encoded by polynucleotides that constitute part of DNA of barley chromosomes (1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes) are immobilized. The polypeptides immobilized on the support may solely be antibodies against the polypeptides encoded by polynucleotides constituting part of barley chromosomal DNA, or other antibodies may additionally be immobilized on the support. Such additional antibodies are not particularly limited. For example, antibodies encoded by polynucleotides with the base sequences originating in non-barley organisms may be immobilized.

As used herein, the term “antibodies” refers to immunoglobulins that can recognize and bind to specific antigens. However, the term does not necessarily mean antibody molecules as a whole, but also refers to part of antibody molecules including antigen binding sites.

It is preferable that the support include a plurality of immobilizing regions, and that different kinds of antibodies be immobilized in these regions. Further, the antibodies immobilized in each region are not necessarily required to be of the same kind. More than one kind of antibodies may be immobilized in each region.

The support is not particularly limited as long as it can immobilize antibodies, i.e., proteins, and it may have any shape and may be made of any material. Examples of a support material generally include: inorganic materials such as glass and silicon wafer; natural polymers such as paper; synthetic polymers such as nitrocellulose and nylon; and gels using synthetic polymers or natural polymers. The shape of the support is not particularly limited as long as it provides enough area to support the polynucleotides. Generally, those with a two-dimensional plane, for example, such as a substrate with little or no flexibility, a flexible membrane, or a flexible substrate with intermediate flexibility can be preferably used. The thickness of the substrate or membrane is not particularly limited either, and it can be suitably set according to the material or use of the substrate or membrane. Various types of beads may be used as supports.

[Antibodies Immobilized on a Support of the Polypeptide Detection Instrument]

In a polypeptide detection instrument according to the present invention, at least one antibody against a polypeptide encoded by a polynucleotide selected from the following polynucleotides (a) or (b) is immobilized on a support.

(a) Polynucleotides with base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

(b) Polynucleotides with a combination of base sequences constituting part of barley chromosomal DNA, or variants thereof with the substitution, deletion, insertion, and/or addition of one or more bases.

As used herein, a polynucleotide with a base sequence constituting part of barley chromosomal DNA is not particularly limited as long as it is a polynucleotide that has a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes, and that includes a protein-coding region. Further, a polynucleotide with a combination of base sequences constituting part of barley chromosomal DNA refers to a polynucleotide in which a base sequence constituting part of the entire base sequences of chromosomal DNA of barley 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes is joined to non-continuous base sequences constituting other parts of these chromosomes, and in which a protein-coding region is contained. For example, base sequences from two different parts of the chromosomes may constitute the polynucleotide, or three or more base sequences may join together to form the polynucleotide. Specifically, for example, cDNA with a plurality of exons from a protein-coding gene on barley chromosomal DNA can be regarded as a polynucleotide with a combination of base sequences constituting part of barley chromosomal DNA. However, the polynucleotide is not just limited to this specific example.

A variant with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotide with a base sequence, or a combination of base sequences, constituting part of barley chromosomal DNA may be a polynucleotide that has been mutated on purpose, or a polynucleotide that exists in nature. For example, think of a base sequence of chromosomal DNA in a specific variety of barley. Comparing this base sequence with those of other varieties, no sequence is completely identical. Rather, these sequences are variants with the substitution, deletion, insertion, and/or addition of one or more bases. The polypeptide encoded by a polynucleotide with such variant base sequence may have the same amino acid sequence as the polypeptide encoded by a polynucleotide with a non-variant base sequence. Further, the polypeptides encoded by the polynucleotides with variant and non-variant base sequences may differ from each other with the substitution of some of the amino acids, or most of or all of the amino acid sequences may be different between these polypeptides.

Polynucleotides that encode polypeptides used for production of antibodies immobilized on a support of a polypeptide detection instrument according to the present invention are preferably polynucleotides with the base sequences of SEQ ID NO: 1 through 5780, or variants with the substitution, deletion, insertion, and/or addition of one or more bases in the polynucleotides with the base sequences of SEQ ID NO: 1 through 5780. (Such polynucleotides and variants will be referred to as polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780.)

The base sequences of SEQ ID NO: 1 through 5780 are base sequences of the barley EST (expressed sequence tag) independently developed by the inventors. The inventors have previously confirmed that a polynucleotide with the base sequences of SEQ ID NO: 1 through 770, a polynucleotide with the base sequences of SEQ ID NO: 771 through 1754, a polynucleotide with the base sequences of SEQ ID NO: 1755 through 2642, a polynucleotide with the base sequences of SEQ ID NO: 2643 through 3324, a polynucleotide with the base sequences of SEQ ID NO: 3325 through 4320, a polynucleotide with the base sequences of SEQ ID NO: 4321 through 4962, and a polynucleotide with the base sequences of SEQ ID NO: 4963 through 5780 are mapped on 1H, 2H, 3H, 4H, 5H, 6H, and 7H chromosomes, respectively.

It follows from this that the base sequences of SEQ ID NO: 1 through 5780 are partial sequences of barley cDNA. Thus, if polynucleotides with the base sequences of SEQ ID NO: 1 through 5780 contained protein-coding regions, antibodies against polypeptides encoded by these polynucleotides can be immobilized on the support. Further, variants with the substitution, deletion, insertion, and/or addition of one or more bases in the base sequences of SEQ ID NO: 1 through 5780 constitute variant base sequences of barley cDNA. Thus, if these polynucleotides with variant base sequences contained protein-coding regions, antibodies against polypeptides encoded by these polynucleotides can be immobilized on the support. The polynucleotides with variant base sequences may be polynucleotides that have been mutated on purpose, or polynucleotides that exist in nature.

Polynucleotides that encode polypeptides used for production of antibodies immobilized on a support of a polypeptide detection instrument according to the present invention may be part of the polynucleotides or the like with the base sequences of SEQ ID NO: 1 through 5780. Since the base sequence of such partial polynucleotide is a partial base sequence of barley cDNA, antibodies against polypeptides encoded by such partial polynucleotides can be immobilized on the support if the partial polynucleotides contained a protein-coding region.

Further, a polynucleotide that encodes a polypeptide used for production of an antibody immobilized on a support of a polypeptide detection instrument according to the present invention may be a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. The remaining base sequences of the polynucleotide are not limited. For example, since the base sequences of SEQ ID NO: 1 through 5780 are partial sequences of barley cDNA, these base sequences do not have the sequences on either end as originally found in the full length cDNA. Thus, a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780, and which additionally includes the cDNA sequences on the both ends or one end as originally found in the full length cDNA can be regarded as a polynucleotide whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. Further, vectors such as plasmids and BACs (bacterial artificial chromosomes) that have incorporated all of or part of the polynucleotide or the like with the base sequences of SEQ ID NO: 1 through 5780, and polynucleotides in which the partial sequence is ligated to arbitrary base sequences can also be regarded as polynucleotides whose partial sequence comprises all of or part of the base sequences of SEQ ID NO: 1 through 5780. Such polynucleotides at least include all of or part of the polynucleotide or the like with the base sequences of SEQ ID NO: 1 through 5780, i.e., part of barley cDNA. Therefore, antibodies against polypeptides encoded by such polynucleotides can be immobilized on the support if the polynucleotides contained a protein-encoding region.

Further, polynucleotides that encode polypeptides used for production of antibodies immobilized on a support of a polypeptide detection instrument according to the present invention may be polynucleotides or the like whose partial sequences comprise all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n (where n is an odd number), and all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780. As described above, the base sequences of SEQ ID NO: 1 through 5780 are EST sequences of barley, and comprise sequences that can be read by sequencing the cloned cDNA from the both ends only once. In other words, the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 among the base sequences of SEQ ID NO: 1 through 5780 are base sequences that are read from the both ends of the cDNA of the same clone. As such, these base sequences can realize a full length cDNA base sequence, which corresponds to all of or part of the full length cDNA. Thus, polynucleotides or the like whose partial sequences comprise all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n (where n is an odd number), and all of or part of the base sequence, or a variant thereof, of SEQ ID NO: n+1, from among the base sequences of SEQ ID NO: 1 through 5780 can be regarded as polynucleotides with full length cDNA that comprises the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1, or polynucleotides that comprise part of the full length cDNA. Further, the polynucleotides may be polynucleotides in which vector sequences or arbitrary base sequences for example are ligated to the both ends or one end of the full length cDNA or polynucleotides that comprise part of the full length cDNA. Further, the polynucleotides may be variants that have a base substitution or other mutations in sequences other than the base sequences of SEQ ID NO: 1 through 5780, i.e., a middle section of the total cDNA unspecified by SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1. Such polynucleotides include the full length or part of barley cDNA, and therefore antibodies against polypeptides encoded by the polynucleotides can be produced. In particular, when the polynucleotides are full length cDNA or include full length cDNA, antibodies against the entire proteins encoded by the genes located on the barley chromosomes can be produced.

[Peptide Detection Instrument with the Antibodies Immobilized in Regions that are Arranged in the Chromosomal Order of the Polynucleotides Encoding Polypeptides Used for Production of the Antibodies]

As described above, the chromosomal order of polynucleotides on barley chromosomes (1H, 2H, 3H, 4H, 5H, 6H, or 7H) (distance from the short arm end of chromosome) has been specified in clones that have the base sequences of SEQ ID NO: n (where n is an odd number) and SEQ ID NO: n+1 from among the base sequences of SEQ ID NO: 1 through SEQ ID NO: 5780. Thus, if antibodies against the polypeptides encoded by the polynucleotides with the base sequences of SEQ ID NO: 1 through SEQ ID NO: 5780 were placed according to the order, as represented in Table 1-1 through Table 7-9, of the polynucleotides encoding the polypeptides, it would be possible to place the polypeptides according to the order from the short arm end of 1H chromosome to the long arm end of 7H chromosome, i.e., chromosomal order. For clones that have the same order from the short arm end, the order by which these clones are placed is not particularly limited. Once the precise order of these clones were specified by a future study, these clones will be able to be placed accordingly. Note that, the reference point (origin) on the chromosome is not just limited to the short arm end of 1H chromosome. Any position on a chromosome can be used as a reference point.

With the antibodies immobilized on a support in the order the polynucleotides encoding the polypeptides used for production of the antibodies are arranged on the chromosomes, the location and extent of recombination on chromosomes can be checked for genes from which the expressed proteins originate. That is, the genotypes of the parents are directly inherited as the haplotype in portions of the chromosomes where no recombination has occurred. As a result, the genotype of a trait conferred to the selected individuals can be easily determined, with the result that the efficiency of breeding is improved.

[Polypeptide Detection Instrument with the Antibodies Immobilized in Regions appended with Chromosomal Order Information of the Polynucleotides Encoding Polypeptides Used for Production of the Antibodies]

The regions in which the antibodies are immobilized (spots) may be appended with information indicative of the order in which the polynucleotides encoding the polypeptides used for production of the antibodies are arranged on barley chromosomes. The spots may be arranged in any way as long as they are appended with the order information. With the order information added to the spots, the data obtained from the spots can be rearranged in the chromosomal order even when the spots are randomly placed on a support. Note that, the order information added to the spots can follow the foregoing criteria used to place the antibodies.

Adding order information enables the spots to be arranged in an arbitrary order in a polypeptide detection instrument of an array type, in which more than one antibody is immobilized on a support such as a membrane or a glass slide. Further, the chromosomal order information of individual antibodies can also be added in a polypeptide detection instrument that employs a collection of beads (bead array) in which the antibody is immobilized on each bead serving as a support.

[Examples of a Polypeptide Detection Instrument according to the Present Invention]

Various conventional techniques that are designed for detection of protein (polypeptide) using antibodies can suitably be used in a polypeptide detecting instrument according to the present invention.

As an example of a peptide detection instrument according to the present invention, the following describes an antibody array of a bead type. The substance immobilized on the beads serving as supports is not limited to antibody. Other polypeptides or polynucleotides may be immobilized as well. As such, a gene detection instrument, a gene polymorphism detection instrument, and a polypeptide-interacting substance detection instrument according to the present invention can also be realized as bead arrays.

In an antibody array of a bead type, it is preferable that one kind of antibody be immobilized on each bead, and that each bead be appended with barley chromosomal order information of polynucleotides encoding polypeptides used for production of the antibodies. For example, by using each well of a micro titer plate as a small vessel, a plurality of beads with identification code (information of the immobilized antibodies, position information on the chromosomes, etc.) is placed in each well. By reading the identification code, information regarding identity of the immobilized antibodies can be specified. With use of a two-wavelength laser beam, 100 kinds of beads can be quantified. Further, since the technique allows for detection in a liquid phase, it is particularly effective in efficiently quantifying proteins. A representative example is Luminex, which is a fluorescent micro beads array system manufactured by HitachiSoft.

(5) Use of a Detection Instrument according to the Present Invention

Use of a detection instrument according to the present invention is not particularly limited. For example, the detection instrument can be suitably used to identify chromosome fragments including a target trait (identification of genotype), from hybrids obtained by crossing Triticeae species. Further, the detection instrument can be suitably used to screen for a variety with a target trait, from hybrids obtained by crossing Triticeae species for variety improvement. For these purposes, it is preferable that the polynucleotides or other substances immobilized on the support such as an array be arranged in the chromosomal order.

In conventional arrays, the polynucleotides or other substances immobilized on the support are randomly arranged. This enables the expression level or other profiles of the immobilized polynucleotides etc. to be individually analyzed. In hybrids, individual genes are inherited in units of blocks, from a point of cross over to the next point of cross over, on the chromosomes. Therefore, for the genotype identification or selection in variety improvement, etc., it is necessary to determine the location and extent of recombination and the presence or absence of unnecessary recombination, in addition to finding individual traits. Thus, conventional arrays with randomly arranged DNA fragments cannot be used efficiently for the screening in variety improvement, etc.

On the other hand, in a detection instrument of the present invention, the polynucleotides or other substances immobilized on the support are arranged in the chromosomal order. Thus, with a detection instrument of the present invention, the locations of recombination on the chromosomes can be found, if any, with a single round of testing. This allows for accurate selection of individuals with desirable traits from a segregating population of hybrid individuals. Further, with a detection instrument according to the present invention, chromosomal recombinations in the hybrid generation can easily be estimated. This allows a group of genes to be introduced in units of blocks, or genes in the blocks to be modified.

Further, with a detection instrument according to the present invention, the recombination patterns, i.e., the location and type of recombination on the chromosomes can be accurately grasped. Thus, by identifying conserved regions of chromosomes where recombination frequency is small in the population of hybrids or natural population, recombination can be efficiently promoted only in these regions of the chromosomes.

Referring to FIG. 1 and FIG. 2, the following briefly describes an example of a method by which target traits are screened for using a detection instrument according to the present invention. For example, a DNA micro array was used in which polynucleotides with partial base sequences of chromosomal DNA of barley were placed and immobilized on a support in the chromosomal order. In the DNA micro array, solid spots X in FIG. 1( a) indicate that genes that confer brewing characteristics are expressed, and hatched spots Y in FIG. 1( b) indicates expression of genes that confer disease-resistance.

In the DNA micro array, the spots are arranged in a chromosomal order, and therefore the positions of spots X and Y are fixed. For example, in FIG. 1( a), the spots X are fixed at the first, second, fifth, and sixth positions of the first row, and at the ninth and tenth positions of the bottom row. The spots Y are fixed at the third and fourth positions of the first row, as shown in FIG. 1( b).

It is assumed here that segregating populations as represented by four micro arrays in the bottom of FIG. 2 were obtained from a cross between a variety expressing the brewing genes as indicated by spots X (corresponding to the upper left DNA micro array in FIG. 2) and a variety expressing the disease-resistant genes as indicated by spots Y (corresponding to the upper right DNA micro array in FIG. 2), for example. From the result of analysis using these DNA micro arrays, varieties expressing both the brewing genes and disease-resistant genes can be screened for from the segregating populations (variety corresponding to the upper left DNA micro array circled by a dotted line in the lower portion of FIG. 2).

Further, whether the chromosome fragments have derived from which parent can easily be determined also for other regions of the genome. Thus, a backcross, for example, between a hybrid and the variety shown in FIG. 1( a) easily allows for selection and development of varieties having all of the expressed spots as illustrated in FIG. 1( a), i.e., the first, second, fifth, and sixth spots of the first row, and the ninth and tenth spots of the bottom row, as well as the third and fourth spots of the first row as shown in FIG. 1( b).

In using a detection instrument according to the present invention for genotyping (whether chromosome sites originated from which parent) or detection of traits (quantitative and qualitative) linked to the genetic markers, the accuracy of data can be improved by increasing the density of the genetic markers with increased numbers of polynucleotides, polypeptides, or antibodies immobilized on a support of a detection instrument according to the present invention.

The density of polynucleotides, polypeptides, or antibodies (a distance between adjacent polynucleotides etc.) immobilized on the support is preferably no greater than 30 cM, more preferably no greater than 15 cM, or particularly preferably no greater than 10 cM, in order to confirm linkage between two adjacent markers.

For the entire barley genome, the number of polynucleotides, polypeptides, or antibodies immobilized on the support is preferably no less than 50, more preferably no less than 100, and particularly preferably no less than 150. When performing genotyping for a Mendelian segregating population of barley with a resolution as high as 1 cM, the number of polynucleotides, polypeptides, or antibodies immobilized on the support is preferably no less than 1500.

Example

With the polynucleotides immobilized on a support of a detection instrument according to the present invention, assessment was made as to whether the genotype of each parent occurs in which part of 1H chromosome in 5 lines of hybrids (DHHS1, DHHS2, DHHS3, DHHS4, and DHHS5) obtained from a cross between malting barley (Haruna Nijo), and wild type barley (H602). That is, locations of recombination were determined.

[Genetic Markers]

Nine genetic markers mapped on 1H chromosome of barley were used. Clones: bah47d23, baa119i12, bah45i3, bags30g20, BaGS13F08, baet45e0410, bah16m01, BaH56B06, and BaH39L18.

[Primers]

Table. 29 below shows the primers used in this experiment.

TABLE 29 Forward Primer Haruna Nijo SEQ ID SEQ ID Clones Forward Primer H602 NO: Reverse Primer NO: bah47d23 AGATGGAGGGGCCCTGTGCAT 22343 CTGTGGGAAAGCCTACATCC 5880 TAGATGGAGGGGCCCTGTGTAT 22344 baal19i12 TCAGAGAGGTGAATCTGGGTCAA 22345 GAACTGAGGCGTGCACATAA 6012 TTCAGAGAGGTGAATCTGGGTTAA 22346 bah45i13 CCATGACCAGCAAAAGCAGTCC 22347 GCAAATCAGTTGCTGGAACA 6340 CCATGACCAGCAAAAGCAGCCC 22348 bags30g20 GGACTACGTACGGACTGAAATAG 22349 GGTTCCATTCCTGCAGATGT 6632 GGACTACGTACGGACTGAAACAG 22350 BaGS13F08 TCACAAGGTAACCAAAACAATTCGA 22351 CTCAGGCAATGCATCAAATG 6760 ATCACAAGGTAACCAAACAATTTGA 22352 baet45E0410 CCGGACTTGACAAGCGGTAATTG 22353 CGGCTCTCCATAGACTGCTC 6800 CGGACTTGACAAGCGGTAAGTG 22354 bah16m01 CATGGGGGAGGTTTTGGCTCTTT 22355 AAGACCTCACTCCAAAGCGA 6904 ATGGGGGAGGTTTTGGCTCGTT 22356 BaH56B06 CTTTTTGGTCCTCAGTCCTCATTG 22357 AGATCCGCTACTGCTTGGAA 7064 TTTTTGGTCCTCAGTCCTCACTG 22358 BaH39L18 GCTTCTAGACGCAGACAAGCTG 22359 GTATGCTTGCAGGAAGGCTC 7316 AGCTTCTAGACGCAGACAAGTTG 22360

As the reverse primers, the primers used to find polymorphism in 1H chromosomes, shown in Tables 8-1 through 8-8, were used. Specifically, bah47d23, baa119i12, bah45i13, bags30g20, BaGS13F08, baet45e0410, bah16m01, BaH56B06, and BaH39L18 are primers with the base sequences of SEQ ID NO: 5880, SEQ ID NO: 6012, SEQ ID NO: 6340, SEQ ID NO: 6632, SEQ ID NO: 6760, SEQ ID NO: 6800, SEQ ID NO: 6904, SEQ ID NO: 7064, and SEQ ID NO: 7316, respectively.

The forward primers were designed based on SNP-containing portions of the base sequences. Specifically, the third base from the 3′ end of the primer sequence was designated as a base characterizing “Haruna Nijo” or “H602”. For the first and second bases on the 3′ end, the same kind of base but different from one found in the original base sequence was used. Specifically, when the base in the original sequence was A, a purine, G, was selected (and vice versa), and when the base in the original sequence was C, a pyrimidine, T, was selected (and vice versa).

For the designing of forward primers, the base sequences in the vicinity of the SNPs of the SNP markers, shown in Tables 22-1 through 22-20, mapped on 1H chromosome were used. Specifically, the forward primers (SEQ ID NO: 22343 (Haruna Nijo) and SEQ ID NO: 22344 (H602)) for bah47d23 had the base sequence of SEQ ID NO: 17380. The forward primers (SEQ ID NO: 22345 (Haruna Nijo) and SEQ ID NO: 22346 (H602)) for baa119i12 had the base sequence of SEQ ID NO: 17419. The forward primers (SEQ ID NO: 22347 (Haruna Nijo) and SEQ ID NO: 22348 (H602)) for bah45i13 had the base sequence of SEQ ID NO: 17556. The forward primers (SEQ ID NO: 22349 (Haruna Nijo) and SEQ ID NO: 22350 (H602)) for bags30g20 had the base sequence of SEQ ID NO: 17669. The forward primers (SEQ ID NO: 22351 (Haruna Nijo) and SEQ ID NO: 22352 (H602)) for BaGS13F08 had the base sequence of SEQ ID NO: 17723. The forward primers (SEQ ID NO: 22353 (Haruna Nijo) and SEQ ID NO: 22354 (H602)) for baet45e0410 had the base sequence of SEQ ID NO: 17742. The forward primers (SEQ ID NO: 22355 (Haruna Nijo) and SEQ ID NO: 22356 (H602)) for bah16m01 had the base sequence of SEQ ID NO: 17785. The forward primers (SEQ ID NO: 22357 (Haruna Nijo) and SEQ ID NO: 22358 (H602)) for BaH56B06 had the base sequence of SEQ ID NO: 17860. The forward primers (SEQ ID NO: 22359 (Haruna Nijo) and SEQ ID NO: 22360 (H602)) for BaH39L18 had the base sequence of SEQ ID NO: 17958.

[PCR Reaction]

From each of the 5 lines of barley hybrids, genomic DNA was prepared and used as a template. Table 30 shows the composition of the PCR reaction solution. The reaction was performed under the following conditions.

94° C. for 2 minutes

Start of 5 cycles consisting of:

94° C. for 30 seconds;

65° C. for 30 seconds (−1° C./cycle); and

72° C. for 1 minute

Start of 30 cycles consisting of:

94° C. for 30 seconds;

60° C. for 30 seconds; and

72° C. for 1 minute

72° C. for 7 minutes

End of Reaction

TABLE 30 Composition of PCR reaction solution (×1) mQH₂O 6.86 10X Blend Taq buffer 1.00 dNTPs (2.0 mM) 1.00 Primer fwd. (50 μM) 0.02 Primer rev. (50 μM) 0.02 Blend Taq (2.5 U/μl) 0.10 DNA (10 ng/μl) 1.00 Total (μl) 10.00

[Result]

FIG. 3 shows the result. In FIG. 3, regions indicated by “H” (black) are regions originating in Haruna Nijo, and regions indicated by “S” (blank) are regions originating in H602. As can be seen in FIG. 3, in DHHS1, bah47d23 through BaGS13F08 were amplified by the forward primers based on the base sequences of Haruna Nijo, and baet45e0410 through BaH39L18 were amplified by the forward primers based on the base sequence of H602. In other words, in DHHS1, there were recombinations between the genetic markers based on BaGS13F08 and the genetic markers based on baet45e0410.

Similarly, in DHHS2, two recombinations were observed: one between bah47d23 and baa119i12, and one between BaGS13F08 and baet45e0410. In DHHS3, two recombinations were observed: one between bah47d23 and baa119i12, and one between bags30g20 and BaGS13F08. In DHHS4, two recombinations were observed: one between bah47d23 and baa119i12, and one between BaH56B06 and BaH39L18. In DHHS5, all clones had the genotype of H602, suggesting that the entire 1H chromosome of these individuals is very likely to be of the H602 origin.

The polynucleotides used in this Example were not immobilized on a support. However, the forward primers and reverse primers used in this Example may be immobilized, for example, on the Gene Silicon in the chromosomal order, so as to fabricate a detection instrument according to the present invention. It should be apparent to a person ordinary skill in the art that the result obtained in this Example can also be obtained with such a detection instrument.

The embodiments and concrete examples of implementation discussed in the foregoing detailed explanation serve solely to illustrate the technical details of the present invention, which should not be narrowly interpreted within the limits of such embodiments and concrete examples, but rather may be applied in many variations within the spirit of the present invention, provided such variations do not exceed the scope of the patent claims set forth below.

INDUSTRIAL APPLICABILITY

A detection instrument according to the present invention uses polynucleotides that can be used as genetic markers mapped on barley chromosomes. The detection instrument can be used to quickly and comprehensively detect gene expression, gene polymorphism, proteins (polypeptides), and protein (polypeptide)-interacting substances in Triticeae species. This greatly improves the efficiency of screening and breeding of Triticeae species. If the detection instrument were able to grow useful Triticeae species of various characteristics in a short time period, it would be possible to offer a solution to food problems.

Taken together, a detection instrument according to the present invention can be suitably used for breeding of Triticeae species, and is applicable to a wide range of agricultural fields. A detection instrument of the present invention can also be used for the basic research in agriculture and other fields of biology. 

1. A gene detection instrument for detecting expression or polymorphism of genes existing in a genome of Triticeae species, said gene detection instrument comprising a support on which are immobilized at least 50 polynucleotides selected from the group consisting of: (a) polynucleotides with partial base sequences of chromosomal DNA of barley and (b) polynucleotides in which a base sequence constituting part of entire base sequences of chromosomal DNA of barley is joined to non-continuous base sequences constituting other parts of the entire base sequences of chromosomal DNA of barley wherein the polynucleotide immobilized on the support comprises; at least one kind of polynucleotide is selected from the group consisting of: (1) polynucleotides with the base sequences of SEQ ID NO: 1 through 5780; (2) a polynucleotide whose partial sequence comprises all of any one of the polynucleotides set forth in (1); and (3) a polynucleotide whose partial sequence comprises: all of a base sequence of SEQ ID NO: n (where n is an odd number), or in the polynucleotides set forth in (1), and all of a base sequence of SEQ ID NO: n+1, or in the polynucleotides set forth in (1); the polynucleotides being mapped on barley chromosomes for an entire genome of barley, and the polynucleotides being mapped on the support at an average density of no greater than 30 cM; regions on the support in which said at least 50 polynucleotides are respectively immobilized being arranged in the same order as a chromosomal order of said at least 50 polynucleotides immobilized on the support, or information indicative of a chromosomal order of said at least 50 polynucleotides immobilized on the support being appended to regions on the support in which said at least 50 polynucleotides are respectively immobilized.
 2. A gene detection instrument as set forth in claim 1, wherein the polynucleotide immobilized on the support comprises cDNA. 